IVF0018049 (gene) Melon (IVF77) v1

Overview
NameIVF0018049
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description3-epi-6-deoxocathasterone 23-monooxygenase
Locationchr08: 5687088 .. 5692383 (+)
RNA-Seq ExpressionIVF0018049
SyntenyIVF0018049
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAATTCCCACAAAGGACAAAGCTATATACCTAATTTCCTAAATAGACACTCTCCTATACTCCTATTTATATTCCTCTTTTCTCATTTCTCTCTCTTTTTTTCTCTTTGAGAGACACATAAAAACTGGGTCTTTGGCTGCATGAATTGATTTGATAGCCAAGTAAAAGTCTTCTCTCTCTCTCTCTCTCTCTTTTTTTTTTTTTTTTGCCTGGTTGACTCAAGAAAAAGAAAAAGTCGCAATCTTTTTTTGTTTAATAGGGTGTTTGTAGGGTTTAGAGAGAATTGAAAAAAATGGGATTTTGGGTATGGTTTGTGTGTGTTATTTTAGGTTGGTTTTTGTTCAAGAAGAAGAAGAAGATTATTGAGAACAAAGATGGAATTCCTAAAGGGAATTTGGGTTGGCCTTTCTTTGGTGAAACTCTTCAATTCATTTCTTCTGGCTATTCTTCTCGTCCTGTCACCTTCATGGACAAACGCAAATCTCTGTAAGTCAATCTCCCTTTTCTTTTTTCTTTTTTTTCTTTTTCTTTTTTTGTTCTGTCTTCTTAAAAATGAAAAATAATATTAAAAAAGTTTTTTTTTAATTAGTTTTTCAATTATTTCTGATATTTTTCCTTTGGTAGGAAACACCCAGTTACCTATCAAACATATTCATAACCTTACCAAGCATTACATACCCAGCTTTTTTCCTTTTCTTTTTTTCCTTCTCATTCTCTAAGAAAATATTCTTCTTTTTTTCCCTCCCTTTTATTGTTACCATAAATAGAGGATCATATATTTATTTATTTTCTTTCTACATTACTGCATTACAAGAAAGTACACAAAACCCAGCACAAAAATGATGAGTGCCACCATCATTCATTAGCTGGACGTGTTACCTAACTATTTTCACCATAATTAAAGTTGTGGTGGTAATTATGTTTTTTTTCAATTTTTTTATGGTTGTTGTTATGATTAATCCAAGTTAGCAATTAGTTGGATTGATTTTCTATTCAATTGTTTTGGAGAGGCCAGAAGAGAATAAGGAAATGATGATGAGGATTGATAATTGATAATTGATAATTTAATTTAATTTCAAAAAAAAAAAAAAAAAAAAACAACACTGAAGGTCACACATGCAAAGAGAGAAAAAAAGTTTGAATTTTAAACCATAGGAAATATTATTGTTTATTTGTGTTTTTCTTCTCCTGTTTGTTGTAATGAAATGAGGCAATTCTCAATAAGGGATCAGAATTGAAAGCCTAAAAGAACCAAGCATGTACTTTTGAAGAAATTTCCATGTGGGTGAAAATCTTTGGTTGCAATTTTGATCCAAAAAAGTACTAAAAGTGCAGACGTCTGACTCTAGCTGCAGTAACCATAGTCTACTTACTTAGATTGATGAAAAAAATATATATATATAATTAAAGGATTTAATTTTTGTTTGTTATTTATTTTGCAGGTATGGGAATGTGTTTAAAACCAACATTTTGGGGACACCCATAATAGTATCAACTGATTCAGAGGTGAACAAGGTTATTTTACAAAACCATGGGAATATATTTACCCCAGCTTACCCAAAATCAATAACAGAATTACTTGGTACATATTCAATTCTAAGAATGAAAGGGAACGTTCAAAGAAGACTTCATACAATCATTGGAGCCTTCCTTAGGTCTCCTCAACTCAAAGCCCAAATCACTAAAGATATTCAAAATACTGTTCAACTTAGGCTTGCTAATTGGAACAATAACTCTCCATTGCATTTTCAAACTGAAGCCAAACAGGTCTCAATAATCTCCCCTTTTTTTCTTCTTACTTTAAAATATTGTATTATATATATAATATGGGTCATGCATAAAAATTTATATAATTTTATATTTTTTTCAGATCACATTTGAAGTATTGGTGAAGGTGTTGATGAGTGTGGGACCTGGTAAAGAATTGGAGACTTTAAAAAGGGAATTTGAAGAGTTTATTAAAGCTTTGATTTGTATACCTATTAAATTACCTGGAACTAGGCTATATAAATCTTTGAAGGTTTACCTTTTCTTTATTATTATTTGATCAATAAATTAATAGTTTATATTTAATCAATTAACTTGATCTTGTTGTTGGGGTTATGTATACTACAGGCCAGAGAGAAGTTATTGAAAATTGTGGGGAAGATTGTGGAAGAAAGGAAATCGAGGGTGGGGGAGAAGGGTGCACCGAGGGATGCGGTGGACGTGCTCTTACAGGATCACAGTGAGAACCAAGGGCTACCCCTCGATTTCATCAGCAGTCACATCATAGAGCTGATGATTCCCGGGGAGGAGACGGTACCGACGGCCATGACTCTCGCCGTTAAATTTCTCGGTGATTGTCCCCGCGCTCTTGCCCAATTAATGGTACGTTTTCTTTTCTCAGTTTTTTTTATTTCCTTTTTGTGATCCAATGGTTAGGATTTGAATCATGAGGTGGGATGGTGGGTGGAGCCCGTGGGCAAAAACCGTCGGTTTCAAGAGTGTGCTTTCCGATGACCACCATGTTCCAATTAAATAATTATCATGTTACTCACTTAAATATATATTCTCCTTCAAAACATTATACATTTAAATATTGACACTCACATGTCAAACCTAAATAACAATTATAATGATAATAGGTATATCTATGTTTATAAAATGCCAATTTTATTTATAGTGTCTCTTCTAAAATATTAAGGTCAATTAGGGGCAAGCAGTAGAGTTGTTTTGGTTTAGAGAATTGGTGAATCTAGGAAATTATTATGGCTTGGGATGGAATTAATAATCTTTTAGACCTCTTTGGCCTAACATTAATTAATACCAGCTCACTTCGACATTTTCTTTCTAAACAGTAGAATTATTAGGCAATCTTGTATCTAAGATTTACCCTTGTTTGAGTGCCCAACTTCAAATGGGGTGTCTAGTTCTTCTCCATGTTTGGGGTTTCAAAGTATAATAGGTGGTACTTTTCATGTTTGGTTTTATATTATTATTTTAAATTTCCCTTTCTGTTATCGTGTTTGGTATTTTTTTTTTTTAAATTCTAAAATAGTTTGTATTGTATTGAAGTATTTTACACCTCAAAGGCAAATTGTTGTAACTTAATAGACTATTATAACACATGGATCAACTATAATTATAATCGAGTAAACACTGCAAACACTCTATTATTAATCTTTAAAATTGGGGTATAATGGATGTAAAAGTTGTCGAATTAAGTATAATATGTGAGATCACGCAGGATATAGCATCATTGAAGTTTAATAATAGGTTGGTTAGACTGGAAATAAAAAGTGAAAAAAATGTATTTAATTAGATATAAATTTGAGAAGGTAAATATTGATTAGAGACAATCATGATTTGAAAGTTGATTGATTGAATTTGAAGAAGGGAAAAAAAGGGGGGATGGTGTGGGGTTAGAATTAGAAGCAGTATTGACTTTTGTAAAAAGTCGTTATCAGAAGAGTATAGTGAGGGAAGGGATATTATAATTATTATCCGTATGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAAGAAGAGTATAAGTGGAGGTAGACGCACGAGCGGGGGAGCGCACGTGTGAGTGAGTGAGAGAGACAGAGAAAATGAGGCGATGATGTGGAGGTTTCGTGGCCGTACGATATTAAGATTGAGGCGTGTTTGTCAGATAAAAGGGACCACCAATTAACTCCTTGGCTGCGCGTGTAGAGACCATTACCTGTCTCATTGTCCCCTTTTCATTTTCCTTTTCTATTCTTAAAATTATCTCTCTTTTTCTTTTTCAACTCTCACTTCTTTCTTTTTCTTCTTCTTCTTCTAATTCCCCTTTTTCTTTAATTCATAACTTATAACATTATATATCCATTTAAATTCCATCTTGTCATTAAAATCCTTTAATTAGGATTCACATCTTTGGATTGGATCGGAAATACCGTAACATTACGAACTTCTTTGTATATTATAATTATTTGAATTTGGTGGGGTTTCTCAAATTCTCTCCTTTTGTTTGGTTGATGAAATTAGGAAGAGAATATGAAACTGAAGAAGGAGAAGGATGGATCTGGGGAGGAGTATACGTGGACAGATTACATGTCTTTGCAATTTACTCAAAATGTAAGCATAATTAACACTTCAATTCTCACACCAAAAAGTACTGAAAATTTTCTTCTTTATGCTGATTGATTTTGTTTACTCTTCCATTTTCATTTAGGTGATTAGTGAAACATTAAGAATGGCTAATATCGTCAATGGGGTTTGGAGAAAAGCTCAAAAAGATGTGAAAATCAAAGGTATGGTAAGGGAATCTATACCTTTTTTTATTACAACAATTACAAGGATAATTGACTTTTTAAGTGATATTGGTGTCTTATTCATCTAGTTATGCTCTAACAAGTGTGTTTCTCATTTTTTTTATTGGGGTTTCATCGTTGTATATGATGGCAGACTATTTAATACCGCAAGGATGGTGCGTCCTGGCTTCCTTTATTTCAGTTCATATGGATGAAAAGAACTATGCAAACCCACATGAGTTTGATCCATGGAGATGGGAGGTACGATAAAAGAGTGGAAAATGTATTTGCAAGTTTGTGACTTTATTAATCATATCTTGTTTCGTTTTAATCTTTATGAATGATTGAAGTATGGCTGCAATTGTAAAAAATATAAACAGGAAAATTGTCTGCTACAAACAACCATAACTTTACACCTTTTGGAGGGGGACAGAGGCTATGTCCTGGAGTCGAACTCACCAGGCTCGAAATCTCAATTTTCCTTCATCATCTTGTCACGACCTACAGGTACTCTCTCTGTCTTGCATTTAAGAAAAAAATATCTACAAAGAGAGACTGGTTTTAATTGAAACCAATCAGAAGTTGTGGGGGAAAATGTCATTATGATAATAGTAACATTACATAACTGCAAATGAAAGCAGATGGGAAGCTGAAAAGGATGAAATCGTCCATTTTCCGACAGTCAAGATGAGAAGGAAGCTGCCAATCACAGTCACAACCTTAAGTTCTTGACTGGATTCAATCATCTCAACAACTTTGATACCAAGCTGTCGAGGAAATAAGGCGAAAAAAATTTGCCACACACAGATGTAAATGTATTAAATGACCGTAACTGTACTAATACAGGAATTCTAGTTTTACTTTACATGGAGCTCTACTCTTACCATTCTGAATTATGATTCTTGTTCGTGCGTCGCCATCTTTTTGACAGTTCAAAGGGGTCCTTTTTTGTCCATTTCCTCCATTGAATAGCATTCATTGTACCCAACTCCATGTTTAAAGTTTATTTGTTT

mRNA sequence

CTAATTCCCACAAAGGACAAAGCTATATACCTAATTTCCTAAATAGACACTCTCCTATACTCCTATTTATATTCCTCTTTTCTCATTTCTCTCTCTTTTTTTCTCTTTGAGAGACACATAAAAACTGGGTCTTTGGCTGCATGAATTGATTTGATAGCCAAGTAAAAGTCTTCTCTCTCTCTCTCTCTCTCTTTTTTTTTTTTTTTTGCCTGGTTGACTCAAGAAAAAGAAAAAGTCGCAATCTTTTTTTGTTTAATAGGGTGTTTGTAGGGTTTAGAGAGAATTGAAAAAAATGGGATTTTGGGTATGGTTTGTGTGTGTTATTTTAGGTTGGTTTTTGTTCAAGAAGAAGAAGAAGATTATTGAGAACAAAGATGGAATTCCTAAAGGGAATTTGGGTTGGCCTTTCTTTGGTGAAACTCTTCAATTCATTTCTTCTGGCTATTCTTCTCGTCCTGTCACCTTCATGGACAAACGCAAATCTCTGTATGGGAATGTGTTTAAAACCAACATTTTGGGGACACCCATAATAGTATCAACTGATTCAGAGGTGAACAAGGTTATTTTACAAAACCATGGGAATATATTTACCCCAGCTTACCCAAAATCAATAACAGAATTACTTGGTACATATTCAATTCTAAGAATGAAAGGGAACGTTCAAAGAAGACTTCATACAATCATTGGAGCCTTCCTTAGGTCTCCTCAACTCAAAGCCCAAATCACTAAAGATATTCAAAATACTGTTCAACTTAGGCTTGCTAATTGGAACAATAACTCTCCATTGCATTTTCAAACTGAAGCCAAACAGATCACATTTGAAGTATTGGTGAAGGTGTTGATGAGTGTGGGACCTGGTAAAGAATTGGAGACTTTAAAAAGGGAATTTGAAGAGTTTATTAAAGCTTTGATTTGTATACCTATTAAATTACCTGGAACTAGGCTATATAAATCTTTGAAGGCCAGAGAGAAGTTATTGAAAATTGTGGGGAAGATTGTGGAAGAAAGGAAATCGAGGGTGGGGGAGAAGGGTGCACCGAGGGATGCGGTGGACGTGCTCTTACAGGATCACAGTGAGAACCAAGGGCTACCCCTCGATTTCATCAGCAGTCACATCATAGAGCTGATGATTCCCGGGGAGGAGACGGTACCGACGGCCATGACTCTCGCCGTTAAATTTCTCGGTGATTGTCCCCGCGCTCTTGCCCAATTAATGGAAGAGAATATGAAACTGAAGAAGGAGAAGGATGGATCTGGGGAGGAGTATACGTGGACAGATTACATGTCTTTGCAATTTACTCAAAATGTGATTAGTGAAACATTAAGAATGGCTAATATCGTCAATGGGGTTTGGAGAAAAGCTCAAAAAGATGTGAAAATCAAAGACTATTTAATACCGCAAGGATGGTGCGTCCTGGCTTCCTTTATTTCAGTTCATATGGATGAAAAGAACTATGCAAACCCACATGAGTTTGATCCATGGAGATGGGAGGAAAATTGTCTGCTACAAACAACCATAACTTTACACCTTTTGGAGGGGGACAGAGGCTATGTCCTGGAGTCGAACTCACCAGGCTCGAAATCTCAATTTTCCTTCATCATCTTGTCACGACCTACAGGTACTCTCTCTGTCTTGCATTTAAGAAAAAAATATCTACAAAGAGAGACTGGTTTTAATTGAAACCAATCAGAAGTTGTGGGGGAAAATGTCATTATGATAATAGTAACATTACATAACTGCAAATGAAAGCAGATGGGAAGCTGAAAAGGATGAAATCGTCCATTTTCCGACAGTCAAGATGAGAAGGAAGCTGCCAATCACAGTCACAACCTTAAGTTCTTGACTGGATTCAATCATCTCAACAACTTTGATACCAAGCTGTCGAGGAAATAAGGCGAAAAAAATTTGCCACACACAGATGTAAATGTATTAAATGACCGTAACTGTACTAATACAGGAATTCTAGTTTTACTTTACATGGAGCTCTACTCTTACCATTCTGAATTATGATTCTTGTTCGTGCGTCGCCATCTTTTTGACAGTTCAAAGGGGTCCTTTTTTGTCCATTTCCTCCATTGAATAGCATTCATTGTACCCAACTCCATGTTTAAAGTTTATTTGTTT

Coding sequence (CDS)

ATGGGATTTTGGGTATGGTTTGTGTGTGTTATTTTAGGTTGGTTTTTGTTCAAGAAGAAGAAGAAGATTATTGAGAACAAAGATGGAATTCCTAAAGGGAATTTGGGTTGGCCTTTCTTTGGTGAAACTCTTCAATTCATTTCTTCTGGCTATTCTTCTCGTCCTGTCACCTTCATGGACAAACGCAAATCTCTGTATGGGAATGTGTTTAAAACCAACATTTTGGGGACACCCATAATAGTATCAACTGATTCAGAGGTGAACAAGGTTATTTTACAAAACCATGGGAATATATTTACCCCAGCTTACCCAAAATCAATAACAGAATTACTTGGTACATATTCAATTCTAAGAATGAAAGGGAACGTTCAAAGAAGACTTCATACAATCATTGGAGCCTTCCTTAGGTCTCCTCAACTCAAAGCCCAAATCACTAAAGATATTCAAAATACTGTTCAACTTAGGCTTGCTAATTGGAACAATAACTCTCCATTGCATTTTCAAACTGAAGCCAAACAGATCACATTTGAAGTATTGGTGAAGGTGTTGATGAGTGTGGGACCTGGTAAAGAATTGGAGACTTTAAAAAGGGAATTTGAAGAGTTTATTAAAGCTTTGATTTGTATACCTATTAAATTACCTGGAACTAGGCTATATAAATCTTTGAAGGCCAGAGAGAAGTTATTGAAAATTGTGGGGAAGATTGTGGAAGAAAGGAAATCGAGGGTGGGGGAGAAGGGTGCACCGAGGGATGCGGTGGACGTGCTCTTACAGGATCACAGTGAGAACCAAGGGCTACCCCTCGATTTCATCAGCAGTCACATCATAGAGCTGATGATTCCCGGGGAGGAGACGGTACCGACGGCCATGACTCTCGCCGTTAAATTTCTCGGTGATTGTCCCCGCGCTCTTGCCCAATTAATGGAAGAGAATATGAAACTGAAGAAGGAGAAGGATGGATCTGGGGAGGAGTATACGTGGACAGATTACATGTCTTTGCAATTTACTCAAAATGTGATTAGTGAAACATTAAGAATGGCTAATATCGTCAATGGGGTTTGGAGAAAAGCTCAAAAAGATGTGAAAATCAAAGACTATTTAATACCGCAAGGATGGTGCGTCCTGGCTTCCTTTATTTCAGTTCATATGGATGAAAAGAACTATGCAAACCCACATGAGTTTGATCCATGGAGATGGGAGGAAAATTGTCTGCTACAAACAACCATAACTTTACACCTTTTGGAGGGGGACAGAGGCTATGTCCTGGAGTCGAACTCACCAGGCTCGAAATCTCAATTTTCCTTCATCATCTTGTCACGACCTACAGGTACTCTCTCTGTCTTGCATTTAAGAAAAAAATATCTACAAAGAGAGACTGGTTTTAATTGA

Protein sequence

MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEENCLLQTTITLHLLEGDRGYVLESNSPGSKSQFSFIILSRPTGTLSVLHLRKKYLQRETGFN
Homology
BLAST of IVF0018049 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 533.5 bits (1373), Expect = 2.4e-150
Identity = 263/379 (69.39%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 25  ENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVST 84
           E K G IP G+LGWP  GETL FI+ GYSSRPVTFMDKRKSLYG VFKTNI+GTPII+ST
Sbjct: 63  EKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIST 122

Query: 85  DSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQ 144
           D+EVNKV+LQNHGN F PAYPKSITELLG  SIL + G  Q+RLHT+IGAFLRSP LK +
Sbjct: 123 DAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDR 182

Query: 145 ITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFI 204
           IT+DI+ +V L LA+W     +H Q E K++TFE+LVKVLMS  PG+++  LK EFEEFI
Sbjct: 183 ITRDIEASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFI 242

Query: 205 KALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQD--HS 264
           K LICIPIK PGTRLYKSLKA+E+L+K+V K+VEER+  +       D VDVLL+D   S
Sbjct: 243 KGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDS 302

Query: 265 ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGS 324
           E Q  P DF+S  I+E+MIPGEET+PTAMTLAVKFL D P ALA+L+EENM++K+ K   
Sbjct: 303 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 362

Query: 325 GEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISV 384
           GEEY WTDYMSL FTQNVI+ETLRMANI+NGVWRKA KDV+IK YLIP+GWCVLASFISV
Sbjct: 363 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 422

Query: 385 HMDEKNYANPHEFDPWRWE 401
           HMDE  Y NP++FDPWRW+
Sbjct: 423 HMDEDIYDNPYQFDPWRWD 441

BLAST of IVF0018049 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 392.1 bits (1006), Expect = 8.6e-108
Identity = 181/382 (47.38%), Postives = 278/382 (72.77%), Query Frame = 0

Query: 30  IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGN-VFKTNILGTPIIVSTDSEVN 89
           +P G+ GWP  GETL+F+S  YS RP  F+DKR+ L+G+ VF++++ G+  +V+ D+EV+
Sbjct: 49  LPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAEVS 108

Query: 90  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 149
           + +LQ+    F P YP+S+TEL+G  SIL + G +QRR+H ++GAF +S  LK+Q+T D+
Sbjct: 109 RFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTADM 168

Query: 150 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 209
           +  +   L+++ ++S LH Q  AK + FE+LV+ L+ +  G+E++ LK++F+EFI  L+ 
Sbjct: 169 RRRLSPALSSFPDSSLLHVQHLAKSVVFEILVRGLIGLEAGEEMQQLKQQFQEFIVGLMS 228

Query: 210 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 269
           +PIKLPGTRLY+SL+A++K+ +++ +I+ E+++R      PRDA+DVL+ D S+   L  
Sbjct: 229 LPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASPPRDAIDVLIGDGSDE--LTD 288

Query: 270 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 329
           + IS ++I+LMIP E++VP  +TLAVKFL +CP AL QL EEN++LK+ K   GE   WT
Sbjct: 289 ELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQWT 348

Query: 330 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 389
           DYMSL FTQ+VI+ETLR+ NI+ G+ RKA +DV++K +LIP+GWCV   F SVH+D+  Y
Sbjct: 349 DYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDTLY 408

Query: 390 ANPHEFDPWRWEENCLLQTTIT 411
             P++F+PWRW+E  +   + T
Sbjct: 409 DEPYKFNPWRWKEKDMSNGSFT 428

BLAST of IVF0018049 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 391.0 bits (1003), Expect = 1.9e-107
Identity = 188/375 (50.13%), Postives = 269/375 (71.73%), Query Frame = 0

Query: 31  PKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV 90
           P G+LGWP  GET++F+SS YS RP +FMDKR+ +YG VFK++I GT  IVSTD+EVN+ 
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 91  ILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQN 150
           +LQ+    F P YPK++ EL+G  SIL + G++ RR H ++G+FL+SP LKAQI +D+  
Sbjct: 112 VLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHK 171

Query: 151 TVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIP 210
            +   +  W+ + P+  Q  +K + F+VL K L+SV  G++LE LKREFE FI  L+ +P
Sbjct: 172 FLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLP 231

Query: 211 IKLPGTRLYKSLKAREKLLKIVGKIVE----ERKSRVGEKGAPRDAVDVLLQDHSENQGL 270
           I  PGT+L++SL+A++ ++K V +I+E    + K++  +    +D VDVLL+D SE+  L
Sbjct: 232 INFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH--L 291

Query: 271 PLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYT 330
             + I++++I++MIPG ++VP  +TLAVKFL D P AL  L EENMKLK  K+ +GE   
Sbjct: 292 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 351

Query: 331 WTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEK 390
           W DY+SL FTQ VI+ETLRM N++ GV RKA KDV+IK Y+IP+GWC LA   SVH+D+ 
Sbjct: 352 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 411

Query: 391 NYANPHEFDPWRWEE 402
            Y +P++F+PWRW+E
Sbjct: 412 YYESPYKFNPWRWQE 424

BLAST of IVF0018049 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.6e-77
Identity = 154/374 (41.18%), Postives = 233/374 (62.30%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWEEN 403
            +   F+PWRW+ N
Sbjct: 390 KDARTFNPWRWQSN 398

BLAST of IVF0018049 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 280.8 bits (717), Expect = 2.8e-74
Identity = 148/409 (36.19%), Postives = 233/409 (56.97%), Query Frame = 0

Query: 15  FLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNI 74
           FL   K++  + +  +P G  GWPF GET+ ++    ++    FM +  S YG ++++N+
Sbjct: 24  FLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNL 83

Query: 75  LGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAF 134
            G P IVS D+ +N+ ILQN G +F  +YP+SI  +LG +S+L + G++ R + +I   F
Sbjct: 84  FGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNF 143

Query: 135 LRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPG-KELE 194
           L   +L+  + KD++      L +W  NS    Q EAK+ TF ++ K +MS+ PG +E E
Sbjct: 144 LSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETE 203

Query: 195 TLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKG------ 254
            LK+E+  F+K ++  P+ LPGT  +K+L++R  +LK + + +EERK  + E+       
Sbjct: 204 QLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEV 263

Query: 255 APRDAVDVLLQDHSENQ--------------GLPLDFISSHIIELMIPGEETVPTAMTLA 314
              D  ++   DH   Q               L  + I   I+ L+  G ET   A+ LA
Sbjct: 264 KTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALA 323

Query: 315 VKFLGDCPRALAQLMEENMKLKKEKDGSGE-EYTWTDYMSLQFTQNVISETLRMANIVNG 374
           + FL  CP+A+ +L EE++++ + K   GE E  W DY  + FTQ VI+ETLR+ N+V  
Sbjct: 324 IFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRF 383

Query: 375 VWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEE 402
           + RKA KDV+ K Y IP GW VL    +VH+D   Y  P+ F+PWRW++
Sbjct: 384 LHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQ 432

BLAST of IVF0018049 vs. ExPASy TrEMBL
Match: A0A5A7SKB2 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold253G002030 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 403
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402

BLAST of IVF0018049 vs. ExPASy TrEMBL
Match: A0A1S3B2I5 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103485087 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.1e-233
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 403
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402

BLAST of IVF0018049 vs. ExPASy TrEMBL
Match: A0A0A0KH50 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504590 PE=3 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 4.3e-211
Identity = 375/402 (93.28%), Postives = 378/402 (94.03%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW NNS LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANW-NNSQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL               +EYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQL---------------KEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 403
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 385

BLAST of IVF0018049 vs. ExPASy TrEMBL
Match: A0A6J1BUA0 (3-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005423 PE=3 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 1.2e-184
Identity = 322/403 (79.90%), Postives = 362/403 (89.83%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFM 60
           +GFWV F+  +LG + +  K + I+NKDG IPKGNLGWPF GETLQFI+SGYSSRPVTFM
Sbjct: 7   LGFWVCFLSAVLGSWFWMNKMRNIDNKDGRIPKGNLGWPFIGETLQFIASGYSSRPVTFM 66

Query: 61  DKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRM 120
           DKRKSLYG VFKTNILGTPIIVSTDSEVNKV+LQNHGNIF PAYPKSITELLG +SIL+M
Sbjct: 67  DKRKSLYGEVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSITELLGPFSILQM 126

Query: 121 KGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVL 180
            GNVQR++H+IIGAFLRSP LK++ITKDI NTV+L LANWN +  ++ QT+ KQITFEVL
Sbjct: 127 NGNVQRKVHSIIGAFLRSPLLKSRITKDIHNTVRLSLANWNPSHLVYVQTQTKQITFEVL 186

Query: 181 VKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEER 240
           VKVLMSVGPG++L+ LKREFEEFIK LICIP+KLPGTRLYKSLKAREKLLKIV KIVEE+
Sbjct: 187 VKVLMSVGPGEDLKILKREFEEFIKGLICIPLKLPGTRLYKSLKAREKLLKIVRKIVEEK 246

Query: 241 KSRVGEKGAP-RDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 300
           K  + EK AP +DAV+VLL+DH  +QGLPLDFISSH+IELMIPGEETVP AMTLAVKFL 
Sbjct: 247 KWAMAEKAAPAKDAVEVLLEDHEGSQGLPLDFISSHVIELMIPGEETVPMAMTLAVKFLS 306

Query: 301 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 360
           D P ALAQ MEEN +LKK+KD SGEEYTWTD+MSLQFTQNVISETLRMANI+NGVWRKAQ
Sbjct: 307 DSPVALAQTMEENKELKKQKDSSGEEYTWTDFMSLQFTQNVISETLRMANIINGVWRKAQ 366

Query: 361 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEE 402
           KDV+IK YLIPQGWCVLASFISVHMDE+NYANPH+F+PWRWEE
Sbjct: 367 KDVEIKGYLIPQGWCVLASFISVHMDEENYANPHQFNPWRWEE 409

BLAST of IVF0018049 vs. ExPASy TrEMBL
Match: A0A6J1KKY8 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111496682 PE=3 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.8e-183
Identity = 323/410 (78.78%), Postives = 359/410 (87.56%), Query Frame = 0

Query: 1   MGFWVWFVCVI-LGWFLFK-------KKKKIIENKDGIPKGNLGWPFFGETLQFISSGYS 60
           +GFWVWF+ V+ L   LFK         K  I+NK GIPKG+LGWPF GETL FI+SGYS
Sbjct: 5   LGFWVWFLSVVALACLLFKNHHHNNNNNKIHIQNKPGIPKGSLGWPFLGETLAFIASGYS 64

Query: 61  SRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLG 120
           SRPVTFM+ RKSLYG+VFKTNILGTPIIVSTDS+VNKV+LQNHGN F PAYPKSITELLG
Sbjct: 65  SRPVTFMNNRKSLYGSVFKTNILGTPIIVSTDSDVNKVVLQNHGNTFVPAYPKSITELLG 124

Query: 121 TYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAK 180
           ++SIL+M GN Q+R+H+IIGAFL+SP LKA+IT DIQNTV+LRL+NWN +  ++ QTE K
Sbjct: 125 SFSILQMNGNFQKRIHSIIGAFLKSPGLKARITTDIQNTVKLRLSNWNTSKLVYVQTETK 184

Query: 181 QITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIV 240
           QITFEVLVKVLMSVGPG++L+ LKREFEEFIKALICIP+KLPGTRLYKSLKAREKLLKIV
Sbjct: 185 QITFEVLVKVLMSVGPGEDLKILKREFEEFIKALICIPVKLPGTRLYKSLKAREKLLKIV 244

Query: 241 GKIVEERKSRVG--EKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAM 300
            KIVEER+S     EK +PRDAVDVLLQD+   QGLPLDFISSHIIELMIPGEETVP AM
Sbjct: 245 SKIVEERRSTAAEEEKASPRDAVDVLLQDNDVVQGLPLDFISSHIIELMIPGEETVPMAM 304

Query: 301 TLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIV 360
           TLAVKFL DCP ALAQ+MEENM+LKK+KD SGEEYTWTDYMSL FTQNVISETLRMANI+
Sbjct: 305 TLAVKFLSDCPVALAQMMEENMELKKQKDDSGEEYTWTDYMSLLFTQNVISETLRMANII 364

Query: 361 NGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE 401
           NGVWRKAQKDV+IK YLIPQGWCVLASFISVHMDE NY N H+FDPWRWE
Sbjct: 365 NGVWRKAQKDVEIKGYLIPQGWCVLASFISVHMDENNYTNAHQFDPWRWE 414

BLAST of IVF0018049 vs. NCBI nr
Match: XP_008440770.1 (PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo])

HSP 1 Score: 815 bits (2104), Expect = 1.20e-295
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402

BLAST of IVF0018049 vs. NCBI nr
Match: KAA0025709.1 (3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 815 bits (2104), Expect = 1.35e-295
Identity = 402/402 (100.00%), Postives = 402/402 (100.00%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC
Sbjct: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402
           VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402

BLAST of IVF0018049 vs. NCBI nr
Match: XP_004149982.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Cucumis sativus])

HSP 1 Score: 778 bits (2008), Expect = 4.67e-281
Identity = 390/402 (97.01%), Postives = 392/402 (97.51%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNN S LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNN-SQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL EENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLKEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 400

BLAST of IVF0018049 vs. NCBI nr
Match: KAE8647598.1 (hypothetical protein Csa_003166 [Cucumis sativus])

HSP 1 Score: 773 bits (1997), Expect = 1.59e-280
Identity = 388/400 (97.00%), Postives = 390/400 (97.50%), Query Frame = 0

Query: 1   MGFWVWFVCVILGWFLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60
           MG WV FVCVILGWFL KKKKKI+ENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD
Sbjct: 1   MGVWV-FVCVILGWFLLKKKKKIVENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMD 60

Query: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120
           KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK
Sbjct: 61  KRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMK 120

Query: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLV 180
           GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNN S LH QTEAKQITFEVLV
Sbjct: 121 GNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNN-SQLHLQTEAKQITFEVLV 180

Query: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240
           KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK
Sbjct: 181 KVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERK 240

Query: 241 SRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDC 300
           S VGEKGAPRDAVDVLLQDH+ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFL DC
Sbjct: 241 SMVGEKGAPRDAVDVLLQDHNENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLSDC 300

Query: 301 PRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360
           PRALAQL EENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD
Sbjct: 301 PRALAQLKEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKD 360

Query: 361 VKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE 400
           VKIK YLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE
Sbjct: 361 VKIKGYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWE 398

BLAST of IVF0018049 vs. NCBI nr
Match: XP_038881879.1 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Benincasa hispida])

HSP 1 Score: 720 bits (1859), Expect = 3.04e-258
Identity = 357/404 (88.37%), Postives = 380/404 (94.06%), Query Frame = 0

Query: 1   MGFWVWFVCV-ILGWFLFKKKKKI-IENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTF 60
           +GFWVWFV V ILGWF  K+KKK  I N DGIP+GNLGWPFFGETLQFISSGYSSRPVTF
Sbjct: 5   LGFWVWFVSVVILGWFWLKRKKKNHIGNNDGIPRGNLGWPFFGETLQFISSGYSSRPVTF 64

Query: 61  MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILR 120
           MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV+LQNHGNIF PAYPKSITELLGT+SILR
Sbjct: 65  MDKRKSLYGNVFKTNILGTPIIVSTDSEVNKVVLQNHGNIFIPAYPKSITELLGTFSILR 124

Query: 121 MKGNVQRRLHTIIGAFLRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEV 180
           M GNVQ+R+HT+IGAFLRSPQLKAQIT+DIQNTVQLRL+NWN++  ++ QTE KQITFEV
Sbjct: 125 MNGNVQKRVHTLIGAFLRSPQLKAQITQDIQNTVQLRLSNWNSSQLVYLQTETKQITFEV 184

Query: 181 LVKVLMSVGPGKELETLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEE 240
           LVKVLMSVGPG+EL+ LKREFEEFIKALICIPIKLPGTRLYKSLKAR+KLLKIV KIVE+
Sbjct: 185 LVKVLMSVGPGEELKILKREFEEFIKALICIPIKLPGTRLYKSLKARKKLLKIVSKIVED 244

Query: 241 RKSRVGEKGAPRDAVDVLLQDHSENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLG 300
           RKSRV EK APRDAVDVLLQDH E QGLPLDFISSHIIELMIPGEETVP AMTLAVKFL 
Sbjct: 245 RKSRVEEKVAPRDAVDVLLQDHDEIQGLPLDFISSHIIELMIPGEETVPMAMTLAVKFLS 304

Query: 301 DCPRALAQLMEENMKLKKEKDGSGEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQ 360
           DCPRALAQLMEENMKLKK+K+GSGEEY WTDYMSL FTQNVISETLRMANI+NGVWRKAQ
Sbjct: 305 DCPRALAQLMEENMKLKKQKNGSGEEYRWTDYMSLLFTQNVISETLRMANIINGVWRKAQ 364

Query: 361 KDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEEN 402
           KDV+IKDYLIPQGWCVLASFISVHMDEKNYANPH+FDPWRWEEN
Sbjct: 365 KDVRIKDYLIPQGWCVLASFISVHMDEKNYANPHQFDPWRWEEN 408

BLAST of IVF0018049 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 533.5 bits (1373), Expect = 1.7e-151
Identity = 263/379 (69.39%), Postives = 308/379 (81.27%), Query Frame = 0

Query: 25  ENKDG-IPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVST 84
           E K G IP G+LGWP  GETL FI+ GYSSRPVTFMDKRKSLYG VFKTNI+GTPII+ST
Sbjct: 63  EKKKGMIPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIIST 122

Query: 85  DSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQ 144
           D+EVNKV+LQNHGN F PAYPKSITELLG  SIL + G  Q+RLHT+IGAFLRSP LK +
Sbjct: 123 DAEVNKVVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDR 182

Query: 145 ITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFI 204
           IT+DI+ +V L LA+W     +H Q E K++TFE+LVKVLMS  PG+++  LK EFEEFI
Sbjct: 183 ITRDIEASVVLTLASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFI 242

Query: 205 KALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQD--HS 264
           K LICIPIK PGTRLYKSLKA+E+L+K+V K+VEER+  +       D VDVLL+D   S
Sbjct: 243 KGLICIPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDS 302

Query: 265 ENQGLPLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGS 324
           E Q  P DF+S  I+E+MIPGEET+PTAMTLAVKFL D P ALA+L+EENM++K+ K   
Sbjct: 303 EKQSQPSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLEL 362

Query: 325 GEEYTWTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISV 384
           GEEY WTDYMSL FTQNVI+ETLRMANI+NGVWRKA KDV+IK YLIP+GWCVLASFISV
Sbjct: 363 GEEYKWTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISV 422

Query: 385 HMDEKNYANPHEFDPWRWE 401
           HMDE  Y NP++FDPWRW+
Sbjct: 423 HMDEDIYDNPYQFDPWRWD 441

BLAST of IVF0018049 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 391.0 bits (1003), Expect = 1.4e-108
Identity = 188/375 (50.13%), Postives = 269/375 (71.73%), Query Frame = 0

Query: 31  PKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVNKV 90
           P G+LGWP  GET++F+SS YS RP +FMDKR+ +YG VFK++I GT  IVSTD+EVN+ 
Sbjct: 52  PHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVNRA 111

Query: 91  ILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDIQN 150
           +LQ+    F P YPK++ EL+G  SIL + G++ RR H ++G+FL+SP LKAQI +D+  
Sbjct: 112 VLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDMHK 171

Query: 151 TVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALICIP 210
            +   +  W+ + P+  Q  +K + F+VL K L+SV  G++LE LKREFE FI  L+ +P
Sbjct: 172 FLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMSLP 231

Query: 211 IKLPGTRLYKSLKAREKLLKIVGKIVE----ERKSRVGEKGAPRDAVDVLLQDHSENQGL 270
           I  PGT+L++SL+A++ ++K V +I+E    + K++  +    +D VDVLL+D SE+  L
Sbjct: 232 INFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEH--L 291

Query: 271 PLDFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYT 330
             + I++++I++MIPG ++VP  +TLAVKFL D P AL  L EENMKLK  K+ +GE   
Sbjct: 292 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 351

Query: 331 WTDYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEK 390
           W DY+SL FTQ VI+ETLRM N++ GV RKA KDV+IK Y+IP+GWC LA   SVH+D+ 
Sbjct: 352 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 411

Query: 391 NYANPHEFDPWRWEE 402
            Y +P++F+PWRW+E
Sbjct: 412 YYESPYKFNPWRWQE 424

BLAST of IVF0018049 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 291.6 bits (745), Expect = 1.1e-78
Identity = 154/374 (41.18%), Postives = 233/374 (62.30%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWEEN 403
            +   F+PWRW+ N
Sbjct: 390 KDARTFNPWRWQSN 398

BLAST of IVF0018049 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 3.3e-78
Identity = 153/373 (41.02%), Postives = 233/373 (62.47%), Query Frame = 0

Query: 29  GIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNILGTPIIVSTDSEVN 88
           G+P G+LG P  GET Q I +  +  P  F+D+R + YG+VF T++ G P I S D E N
Sbjct: 30  GLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETN 89

Query: 89  KVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAFLRSPQLKAQITKDI 148
           + +LQN G +F  +YP SI  LLG +S+L MKG++ +R+H++  +F  S  +K  +  DI
Sbjct: 90  RFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDI 149

Query: 149 QNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPGKELETLKREFEEFIKALIC 208
              V+  L +W  +S +    EAK+ITFE+ VK LMS  PG+  E+L++E+   I+    
Sbjct: 150 DRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 209

Query: 209 IPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKGAPRDAVDVLLQDHSENQGLPL 268
           +P+ L  T   K+++AR K+ + +  +V +R+    E+GA R   D+L    + + G   
Sbjct: 210 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREE-EEEGAERKK-DMLAALLAADDGFSD 269

Query: 269 DFISSHIIELMIPGEETVPTAMTLAVKFLGDCPRALAQLMEENMKLKKEKDGSGEEYTWT 328
           + I   ++ L++ G ET  T MTLAVKFL + P ALAQL EE+ K++  K  S     W+
Sbjct: 270 EEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSDS-YSLEWS 329

Query: 329 DYMSLQFTQNVISETLRMANIVNGVWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNY 388
           DY S+ FTQ V++ETLR+ANI+ GV+R+A  DV+IK Y IP+GW V +SF +VH+D  ++
Sbjct: 330 DYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHF 389

Query: 389 ANPHEFDPWRWEE 402
            +   F+PWRW++
Sbjct: 390 KDARTFNPWRWQQ 397

BLAST of IVF0018049 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 280.8 bits (717), Expect = 2.0e-75
Identity = 148/409 (36.19%), Postives = 233/409 (56.97%), Query Frame = 0

Query: 15  FLFKKKKKIIENKDGIPKGNLGWPFFGETLQFISSGYSSRPVTFMDKRKSLYGNVFKTNI 74
           FL   K++  + +  +P G  GWPF GET+ ++    ++    FM +  S YG ++++N+
Sbjct: 24  FLILLKRRNRKTRFNLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNL 83

Query: 75  LGTPIIVSTDSEVNKVILQNHGNIFTPAYPKSITELLGTYSILRMKGNVQRRLHTIIGAF 134
            G P IVS D+ +N+ ILQN G +F  +YP+SI  +LG +S+L + G++ R + +I   F
Sbjct: 84  FGEPTIVSADAGLNRFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNF 143

Query: 135 LRSPQLKAQITKDIQNTVQLRLANWNNNSPLHFQTEAKQITFEVLVKVLMSVGPG-KELE 194
           L   +L+  + KD++      L +W  NS    Q EAK+ TF ++ K +MS+ PG +E E
Sbjct: 144 LSHARLRTILLKDVERHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETE 203

Query: 195 TLKREFEEFIKALICIPIKLPGTRLYKSLKAREKLLKIVGKIVEERKSRVGEKG------ 254
            LK+E+  F+K ++  P+ LPGT  +K+L++R  +LK + + +EERK  + E+       
Sbjct: 204 QLKKEYVTFMKGVVSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEV 263

Query: 255 APRDAVDVLLQDHSENQ--------------GLPLDFISSHIIELMIPGEETVPTAMTLA 314
              D  ++   DH   Q               L  + I   I+ L+  G ET   A+ LA
Sbjct: 264 KTEDEAEMSKSDHVRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALA 323

Query: 315 VKFLGDCPRALAQLMEENMKLKKEKDGSGE-EYTWTDYMSLQFTQNVISETLRMANIVNG 374
           + FL  CP+A+ +L EE++++ + K   GE E  W DY  + FTQ VI+ETLR+ N+V  
Sbjct: 324 IFFLQACPKAVEELREEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRF 383

Query: 375 VWRKAQKDVKIKDYLIPQGWCVLASFISVHMDEKNYANPHEFDPWRWEE 402
           + RKA KDV+ K Y IP GW VL    +VH+D   Y  P+ F+PWRW++
Sbjct: 384 LHRKALKDVRYKGYDIPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQ 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M0662.4e-15069.393-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW58.6e-10847.38Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q94IA61.9e-10750.133-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q425691.6e-7741.18Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
O649892.8e-7436.19Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A5A7SKB21.1e-233100.003-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3B2I51.1e-233100.003-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1034850... [more]
A0A0A0KH504.3e-21193.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G504590 PE=3 SV=1[more]
A0A6J1BUA01.2e-18479.903-epi-6-deoxocathasterone 23-monooxygenase isoform X1 OS=Momordica charantia OX=... [more]
A0A6J1KKY83.8e-18378.783-epi-6-deoxocathasterone 23-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
Match NameE-valueIdentityDescription
XP_008440770.11.20e-295100.00PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo][more]
KAA0025709.11.35e-295100.003-epi-6-deoxocathasterone 23-monooxygenase [Cucumis melo var. makuwa][more]
XP_004149982.14.67e-28197.013-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Cucumis sativus][more]
KAE8647598.11.59e-28097.00hypothetical protein Csa_003166 [Cucumis sativus][more]
XP_038881879.13.04e-25888.373-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G36380.11.7e-15169.39Cytochrome P450 superfamily protein [more]
AT3G13730.11.4e-10850.13cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT5G05690.11.1e-7841.18Cytochrome P450 superfamily protein [more]
AT5G05690.23.3e-7841.02Cytochrome P450 superfamily protein [more]
AT3G50660.12.0e-7536.19Cytochrome P450 superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 304..324
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 8..405
NoneNo IPR availablePANTHERPTHR24286:SF2543-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90C1coord: 8..405
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 382..400
score: 30.36
coord: 57..80
score: 23.88
coord: 333..349
score: 32.35
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 20..409
e-value: 1.3E-82
score: 279.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 30..402
IPR001128Cytochrome P450PFAMPF00067p450coord: 31..402
e-value: 1.7E-45
score: 155.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0018049.3IVF0018049.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0009965 leaf morphogenesis
biological_process GO:0042814 monopolar cell growth
biological_process GO:0048441 petal development
biological_process GO:0048443 stamen development
biological_process GO:0016125 sterol metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen