IVF0017706 (gene) Melon (IVF77) v1

Overview


Name	IVF0017706
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	EF-hand domain-containing protein
Location	chr10: 2758759 .. 2759468 (-)
RNA-Seq Expression	IVF0017706
Synteny	IVF0017706

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAACATATTTTGCTCAAGCTTGCTGATTTGATGAATAGCGCCAGGAATCTATCTAACATCCACGCTGATACTGCTCCCTACCCCCTCCTAACTCTGCGCCGGTTATTCACCGTCTTCTCTCTCTCGCCAAAGGATCCTTCCCTCTCTCAACTCCACGTCGGGTCCTTCTCCATGTTCTCTCCCTCGCCGGTCTTTTCTTTCTACGAAAGTTTGAAACCATCCGTCTCACTCTCCTACTCCCTTCTTCGTTTTGCTTCATCGAATCCTTCCGGAGGTTTGTTCTTATTTTAATATTATTTGAATTAATTTCCTTTTTTTTTTTTAGATTCGGTTGAATATGTGTATTTTCTTTCCCGAAAAGATGATACTAGCTGGTTTCAGTAATCAATCAGTAAATCCAGAAATTGGGTTATGGGGAATTTAGGGATTGATTTTAGCGAAGGGTCTCCTTTGTTCCTCTGTTTGGGTCAGCGGAAGAAGTGGCGGAGATGTTCGATCAAACGGCGGCTAGTGCGGCGGAGGTAAAACAAGCTTTTGATGTGTTTGATGTTAATGGCGATGGGTTTATCGACGTTGAAGAATAGCAGAGAGTGATGTGTGTTTTGAGATTCAAAGAAGTAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTGTAACAAAGACGGGAGAATTGATTTGAAGAATTTGTTAAATTGA

mRNA sequence

ATGAAACATATTTTGCTCAAGCTTGCTGATTTGATGAATAGCGCCAGGAATCTATCTAACATCCACGCTGATACTGCTCCCTACCCCCTCCTAACTCTGCGCCGGTTATTCACCGTCTTCTCTCTCTCGCCAAAGGATCCTTCCCTCTCTCAACTCCACGTCGGGTCCTTCTCCATGTTCTCTCCCTCGCCGGTCTTTTCTTTCTACGAAAGTTTGAAACCATCCGTCTCACTCTCCTACTCCCTTCTTCGTTTTGCTTCATCGAATCCTTCCGGAGCGGAAGAAGTGGCGGAGATGTTCGATCAAACGGCGGCTAGTGCGGCGGAGGTAAAACAAGCTTTTGATGTGTTTGATCAGAGAGTGATGTGTGTTTTGAGATTCAAAGAAGTAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTGTAACAAAGACGGGAGAATTGATTTGAAGAATTTGTTAAATTGA

Coding sequence (CDS)

ATGAAACATATTTTGCTCAAGCTTGCTGATTTGATGAATAGCGCCAGGAATCTATCTAACATCCACGCTGATACTGCTCCCTACCCCCTCCTAACTCTGCGCCGGTTATTCACCGTCTTCTCTCTCTCGCCAAAGGATCCTTCCCTCTCTCAACTCCACGTCGGGTCCTTCTCCATGTTCTCTCCCTCGCCGGTCTTTTCTTTCTACGAAAGTTTGAAACCATCCGTCTCACTCTCCTACTCCCTTCTTCGTTTTGCTTCATCGAATCCTTCCGGAGCGGAAGAAGTGGCGGAGATGTTCGATCAAACGGCGGCTAGTGCGGCGGAGGTAAAACAAGCTTTTGATGTGTTTGATCAGAGAGTGATGTGTGTTTTGAGATTCAAAGAAGTAGAAGGGATTGAGAATTGCGAGAAGATGATTCGTAAGTTTGATTGTAACAAAGACGGGAGAATTGATTTGAAGAATTTGTTAAATTGA

Protein sequence

MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMFSPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQTAASAAEVKQAFDVFDQRVMCVLRFKEVEGIENCEKMIRKFDCNKDGRIDLKNLLN

Homology

[Close]

BLAST of IVF0017706 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 9.1e-09
Identity = 33/72 (45.83%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           ++EV+ +F++   S  EVKQAFDVFD            QRV+ +L  K+   +ENC +MI
Sbjct: 120 SKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMI 179

[Close]

BLAST of IVF0017706 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-08
Identity = 34/77 (44.16%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           A+EV+ +F++  AS  EVKQAFDVFD            QRV+ +L FK+   ++NC  MI
Sbjct: 110 AKEVSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMI 169

Query: 153 RKFDCNKDGRIDLKNLL 158
           R  D NKDG+ID    +
Sbjct: 170 RSLDGNKDGKIDFNEFV 186

[Close]

BLAST of IVF0017706 vs. ExPASy TrEMBL
Match: A0A5D3CVK3 (EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G00470 PE=4 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.9e-65
Identity = 140/153 (91.50%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 1   MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF 60
           MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF
Sbjct: 1   MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF 60

Query: 61  SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQTAASAAEVKQAFDVFD-- 120
           SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQT ASAAEVKQAFDVFD  
Sbjct: 61  SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQTGASAAEVKQAFDVFDVN 120

Query: 121 ----------QRVMCVLRFKEVEGIENCEKMIR 142
                     QRVMCVLRFKEVEGIENCEKMIR
Sbjct: 121 GDGFIDVEELQRVMCVLRFKEVEGIENCEKMIR 153

[Close]

BLAST of IVF0017706 vs. ExPASy TrEMBL
Match: A0A5A7T9C9 (Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002800 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.0e-18
Identity = 56/77 (72.73%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAASAAEVKQAFDVFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 89  AEEVAEMFDQTAASAAEVKQAFDVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMI 148

Query: 153 RKFDCNKDGRIDLKNLL 158
            KFD NKDGRID +  +
Sbjct: 149 SKFDYNKDGRIDFEEFV 165

[Close]

BLAST of IVF0017706 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 5.0e-18
Identity = 56/77 (72.73%), Postives = 58/77 (75.32%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAASAAEVKQAFDVFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 98  AEEVAEMFDQTAASAAEVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMI 157

Query: 153 RKFDCNKDGRIDLKNLL 158
            KFD NKDGRID +  +
Sbjct: 158 SKFDYNKDGRIDFEEFV 174

[Close]

BLAST of IVF0017706 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.5e-17
Identity = 55/77 (71.43%), Postives = 57/77 (74.03%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAAS AEVKQAF VFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 97  AEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMI 156

Query: 153 RKFDCNKDGRIDLKNLL 158
           RKFD NKDGRID +  +
Sbjct: 157 RKFDSNKDGRIDFEEFV 173

[Close]

BLAST of IVF0017706 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.8e-12
Identity = 44/76 (57.89%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 94  EEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMIR 153
           EE+ ++FDQTAASAAEVK+AFDVFD            QRV+CVL FKE E I+NC +MI 
Sbjct: 92  EEMCKIFDQTAASAAEVKEAFDVFDVNSDGFIDVEELQRVLCVLGFKEGEAIQNCRRMIN 151

Query: 154 KFDCNKDGRIDLKNLL 158
           KFD N DGRID +  +
Sbjct: 152 KFDENNDGRIDFQEFV 167

[Close]

BLAST of IVF0017706 vs. NCBI nr
Match: TYK15555.1 (uncharacterized protein E5676_scaffold35G00470 [Cucumis melo var. makuwa])

HSP 1 Score: 257 bits (657), Expect = 1.03e-82
Identity = 140/153 (91.50%), Postives = 140/153 (91.50%), Query Frame = 0


Query: 1   MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF 60
           MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF
Sbjct: 1   MKHILLKLADLMNSARNLSNIHADTAPYPLLTLRRLFTVFSLSPKDPSLSQLHVGSFSMF 60

Query: 61  SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQTAASAAEVKQAFDVFD-- 120
           SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQT ASAAEVKQAFDVFD  
Sbjct: 61  SPSPVFSFYESLKPSVSLSYSLLRFASSNPSGAEEVAEMFDQTGASAAEVKQAFDVFDVN 120

Query: 121 ----------QRVMCVLRFKEVEGIENCEKMIR 141
                     QRVMCVLRFKEVEGIENCEKMIR
Sbjct: 121 GDGFIDVEELQRVMCVLRFKEVEGIENCEKMIR 153

[Close]

BLAST of IVF0017706 vs. NCBI nr
Match: KAA0039468.1 (putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 putative calcium-binding protein CML30 [Cucumis melo var. makuwa])

HSP 1 Score: 102 bits (253), Expect = 6.10e-24
Identity = 56/74 (75.68%), Postives = 57/74 (77.03%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAASAAEVKQAFDVFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 89  AEEVAEMFDQTAASAAEVKQAFDVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMI 148

Query: 153 RKFDCNKDGRIDLK 154
            KFD NKDGRID +
Sbjct: 149 SKFDYNKDGRIDFE 162

[Close]

BLAST of IVF0017706 vs. NCBI nr
Match: XP_008459700.2 (PREDICTED: probable calcium-binding protein CML30 [Cucumis melo])

HSP 1 Score: 102 bits (253), Expect = 7.68e-24
Identity = 56/74 (75.68%), Postives = 57/74 (77.03%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAASAAEVKQAFDVFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 98  AEEVAEMFDQTAASAAEVKQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMI 157

Query: 153 RKFDCNKDGRIDLK 154
            KFD NKDGRID +
Sbjct: 158 SKFDYNKDGRIDFE 171

[Close]

BLAST of IVF0017706 vs. NCBI nr
Match: XP_031741770.1 (probable calcium-binding protein CML47 [Cucumis sativus])

HSP 1 Score: 100 bits (249), Expect = 4.36e-22
Identity = 55/74 (74.32%), Postives = 56/74 (75.68%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           AEEVAEMFDQTAAS AEVKQAF VFD            QRVMCVL FKE EGIENCEKMI
Sbjct: 227 AEEVAEMFDQTAASVAEVKQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMI 286

Query: 153 RKFDCNKDGRIDLK 154
           RKFD NKDGRID +
Sbjct: 287 RKFDSNKDGRIDFE 300

[Close]

BLAST of IVF0017706 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 87.8 bits (216), Expect = 2.39e-18
Identity = 48/76 (63.16%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 94  EEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMIR 153
           EEVAEMFD+ AAS  EVK+AFDVFD            QRVMCVL FKE E I+NCE+MIR
Sbjct: 96  EEVAEMFDERAASTVEVKKAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIR 155

Query: 154 KFDCNKDGRIDLKNLL 157
           KFD N DGRID +  L
Sbjct: 156 KFDHNGDGRIDFEEFL 171

[Close]

BLAST of IVF0017706 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 61.6 bits (148), Expect = 6.5e-10
Identity = 33/72 (45.83%), Postives = 45/72 (62.50%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           ++EV+ +F++   S  EVKQAFDVFD            QRV+ +L  K+   +ENC +MI
Sbjct: 120 SKEVSNLFEEKEPSLEEVKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMI 179

[Close]

BLAST of IVF0017706 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 60.8 bits (146), Expect = 1.1e-09
Identity = 34/77 (44.16%), Postives = 46/77 (59.74%), Query Frame = 0


Query: 93  AEEVAEMFDQTAASAAEVKQAFDVFD------------QRVMCVLRFKEVEGIENCEKMI 152
           A+EV+ +F++  AS  EVKQAFDVFD            QRV+ +L FK+   ++NC  MI
Sbjct: 110 AKEVSSLFEEKEASLEEVKQAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMI 169

Query: 153 RKFDCNKDGRIDLKNLL 158
           R  D NKDG+ID    +
Sbjct: 170 RSLDGNKDGKIDFNEFV 186

The following BLAST results are available for this feature:

BLAST of IVF0017706 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	9.1e-09	45.83	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	1.6e-08	44.16	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]

BLAST of IVF0017706 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CVK3	1.9e-65	91.50	EF-hand domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A5A7T9C9	5.0e-18	72.73	Putative calcium-binding protein CML30 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CAB3	5.0e-18	72.73	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A0A0KS94	1.5e-17	71.43	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]
A0A6J1JZ70	1.8e-12	57.89	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]

BLAST of IVF0017706 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK15555.1	1.03e-82	91.50	uncharacterized protein E5676_scaffold35G00470 [Cucumis melo var. makuwa]	[more]
KAA0039468.1	6.10e-24	75.68	putative calcium-binding protein CML30 [Cucumis melo var. makuwa] >TYK00658.1 pu...	[more]
XP_008459700.2	7.68e-24	75.68	PREDICTED: probable calcium-binding protein CML30 [Cucumis melo]	[more]
XP_031741770.1	4.36e-22	74.32	probable calcium-binding protein CML47 [Cucumis sativus]	[more]
XP_038889669.1	2.39e-18	63.16	probable calcium-binding protein CML46 [Benincasa hispida]	[more]

BLAST of IVF0017706 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	6.5e-10	45.83	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	1.1e-09	44.16	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 131..158 score: 11.110167
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 95..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0017706.2	IVF0017706.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding