IVF0017688 (gene) Melon (IVF77) v1

Overview
NameIVF0017688
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr02: 8139747 .. 8141223 (+)
RNA-Seq ExpressionIVF0017688
SyntenyIVF0017688
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAAAATCATCTCTCTTTCTTCTTCTTCTTCTTCTTCTTTAATTGTTTCTCTCTCTTTTTCAATGGAAGAAGTTGCTGTTCCTCCATATTTTATTTGCCCAATTTCGTTACAAATCATGAAAGATCCAGTTACAGCCACCACTGGAATTACTTATGATCGTGAAAGTATTGAGAATTGGTTTTTGACCTCTAAAGATGCAATCTTTTGCCCTCTCACCAAGCAATCTTTGACCAAAGCTTCCGATTTGACACCAAACCACACGCTTCGTCGTCTCATTAAGTCTTGGATGGTTGAGAATGCTTCCCCCGGTGGTGGAGATGATGAATTTCTAACTCCTAGCCCACTTTTAGACAAGACCCGTCTTCGAAAGCTCTTGCGGGAGGTTGCTGTCGCTTCCGATGAGCGCCTTCGCATTGACGCCGTGAAGAAGCTTCATGCTTTGGCTATTGAAAGTGACACGGCAAGTCGAGGCCGTATGGAGGAAGTCGGCGTGGCGAAGGGGATGGTCTTGTTTGTTATAAGACGTTTTAGGGAAGGGAGAATTGGTGGACTTGAGGAAGCTTTGAAGATTCTTAGTCTACTTTCACATTATTCAATTTCTGAAATTAGGTTTCTTGAGTTTTTTGAATCATTGACTTGGGTTTTGGGTTTGGAGATGGAAAATCACACAATAATCAAAAGCTACGCCATTGAAGTGTTGAAGAAAGCAACCGATGTGGCTCCCTCCACCATTCTCACCCAAATAAACCTTGAGTTCTTCAAAAACATCACAAATCTCTTCAGAGAAAAGATCTCAAATTCATCTCTCAAAACAACACTCACAATGCTAATAAACATATGCCCCTGGGGACGAAACCGAGTCAAGCTTGTTGAAGTCGGGGCAGTTTTTGACCTAATCGAACTCGAGCTCGAGAAGCCAGAGAAGAACATAACTGAGCTCATTTTCAACCTCTTGGCCCACCTATGCTCCACGGCAGATGGGAGAGCAGAGCTCATCAGCCATGCGGGGGGCATCGCGGTGGTTTCGAAGAGAATACTTAGAGTTTCGATTGCAACAACCGATCGAGCTATTCAAATTCTATCGTTGATATCGAAACACTCCGCTAGGAAGGATGTTCTTTTGGAGATGTTGAGGGTTGGGGCAGTGTCAAAGCTTTGCATGATAATGCAATCTAATTGTGAGGGATTCTTGAAGGAGAAAGCTAGAGAGATTTTGAGAATGCATTCGAATGTGTGGAGTAATTCTCCATGTATTGGGGTTTATGTGATGACTAGAGACCCTAGATAGTAGATAGCTTCAAAACGTATGATCATTTATTTTAAGTATAATATCAATGTAATATTTTTGGAGGCTTTCCAATTTTTTATGAACAACTGTGTGAAATTTTTGCAGTTGGGCATAAAAAGTTTCCAAAGTTTTAATCCAATTATATGAAATTTTGTCGCAAACTTCTTTGTGTGTGATATTGATTGGGA

mRNA sequence

TTAAAATCATCTCTCTTTCTTCTTCTTCTTCTTCTTCTTTAATTGTTTCTCTCTCTTTTTCAATGGAAGAAGTTGCTGTTCCTCCATATTTTATTTGCCCAATTTCGTTACAAATCATGAAAGATCCAGTTACAGCCACCACTGGAATTACTTATGATCGTGAAAGTATTGAGAATTGGTTTTTGACCTCTAAAGATGCAATCTTTTGCCCTCTCACCAAGCAATCTTTGACCAAAGCTTCCGATTTGACACCAAACCACACGCTTCGTCGTCTCATTAAGTCTTGGATGGTTGAGAATGCTTCCCCCGGTGGTGGAGATGATGAATTTCTAACTCCTAGCCCACTTTTAGACAAGACCCGTCTTCGAAAGCTCTTGCGGGAGGTTGCTGTCGCTTCCGATGAGCGCCTTCGCATTGACGCCGTGAAGAAGCTTCATGCTTTGGCTATTGAAAGTGACACGGCAAGTCGAGGCCGTATGGAGGAAGTCGGCGTGGCGAAGGGGATGGTCTTGTTTGTTATAAGACGTTTTAGGGAAGGGAGAATTGGTGGACTTGAGGAAGCTTTGAAGATTCTTAGTCTACTTTCACATTATTCAATTTCTGAAATTAGGTTTCTTGAGTTTTTTGAATCATTGACTTGGGTTTTGGGTTTGGAGATGGAAAATCACACAATAATCAAAAGCTACGCCATTGAAGTGTTGAAGAAAGCAACCGATGTGGCTCCCTCCACCATTCTCACCCAAATAAACCTTGAGTTCTTCAAAAACATCACAAATCTCTTCAGAGAAAAGATCTCAAATTCATCTCTCAAAACAACACTCACAATGCTAATAAACATATGCCCCTGGGGACGAAACCGAGTCAAGCTTGTTGAAGTCGGGGCAGTTTTTGACCTAATCGAACTCGAGCTCGAGAAGCCAGAGAAGAACATAACTGAGCTCATTTTCAACCTCTTGGCCCACCTATGCTCCACGGCAGATGGGAGAGCAGAGCTCATCAGCCATGCGGGGGGCATCGCGGTGGTTTCGAAGAGAATACTTAGAGTTTCGATTGCAACAACCGATCGAGCTATTCAAATTCTATCGTTGATATCGAAACACTCCGCTAGGAAGGATGTTCTTTTGGAGATGTTGAGGGTTGGGGCAGTGTCAAAGCTTTGCATGATAATGCAATCTAATTGTGAGGGATTCTTGAAGGAGAAAGCTAGAGAGATTTTGAGAATGCATTCGAATGTGTGGAGTAATTCTCCATGTATTGGGGTTTATGTGATGACTAGAGACCCTAGATAGTAGATAGCTTCAAAACGTATGATCATTTATTTTAAGTATAATATCAATGTAATATTTTTGGAGGCTTTCCAATTTTTTATGAACAACTGTGTGAAATTTTTGCAGTTGGGCATAAAAAGTTTCCAAAGTTTTAATCCAATTATATGAAATTTTGTCGCAAACTTCTTTGTGTGTGATATTGATTGGGA

Coding sequence (CDS)

ATGGAAGAAGTTGCTGTTCCTCCATATTTTATTTGCCCAATTTCGTTACAAATCATGAAAGATCCAGTTACAGCCACCACTGGAATTACTTATGATCGTGAAAGTATTGAGAATTGGTTTTTGACCTCTAAAGATGCAATCTTTTGCCCTCTCACCAAGCAATCTTTGACCAAAGCTTCCGATTTGACACCAAACCACACGCTTCGTCGTCTCATTAAGTCTTGGATGGTTGAGAATGCTTCCCCCGGTGGTGGAGATGATGAATTTCTAACTCCTAGCCCACTTTTAGACAAGACCCGTCTTCGAAAGCTCTTGCGGGAGGTTGCTGTCGCTTCCGATGAGCGCCTTCGCATTGACGCCGTGAAGAAGCTTCATGCTTTGGCTATTGAAAGTGACACGGCAAGTCGAGGCCGTATGGAGGAAGTCGGCGTGGCGAAGGGGATGGTCTTGTTTGTTATAAGACGTTTTAGGGAAGGGAGAATTGGTGGACTTGAGGAAGCTTTGAAGATTCTTAGTCTACTTTCACATTATTCAATTTCTGAAATTAGGTTTCTTGAGTTTTTTGAATCATTGACTTGGGTTTTGGGTTTGGAGATGGAAAATCACACAATAATCAAAAGCTACGCCATTGAAGTGTTGAAGAAAGCAACCGATGTGGCTCCCTCCACCATTCTCACCCAAATAAACCTTGAGTTCTTCAAAAACATCACAAATCTCTTCAGAGAAAAGATCTCAAATTCATCTCTCAAAACAACACTCACAATGCTAATAAACATATGCCCCTGGGGACGAAACCGAGTCAAGCTTGTTGAAGTCGGGGCAGTTTTTGACCTAATCGAACTCGAGCTCGAGAAGCCAGAGAAGAACATAACTGAGCTCATTTTCAACCTCTTGGCCCACCTATGCTCCACGGCAGATGGGAGAGCAGAGCTCATCAGCCATGCGGGGGGCATCGCGGTGGTTTCGAAGAGAATACTTAGAGTTTCGATTGCAACAACCGATCGAGCTATTCAAATTCTATCGTTGATATCGAAACACTCCGCTAGGAAGGATGTTCTTTTGGAGATGTTGAGGGTTGGGGCAGTGTCAAAGCTTTGCATGATAATGCAATCTAATTGTGAGGGATTCTTGAAGGAGAAAGCTAGAGAGATTTTGAGAATGCATTCGAATGTGTGGAGTAATTCTCCATGTATTGGGGTTTATGTGATGACTAGAGACCCTAGATAG

Protein sequence

MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASDLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDAVKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSISEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Homology
BLAST of IVF0017688 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.6e-71
Identity = 180/450 (40.00%), Postives = 251/450 (55.78%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           EE+ +P YFICPISL+IMKDPVT  +GITYDR++I  W    +    CP+TKQ L   SD
Sbjct: 6   EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 65

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERL-RIDA 121
           LTPNH LRRLI+ W VEN +   G     TP     K  + + ++ +     E L R + 
Sbjct: 66  LTPNHMLRRLIQHWCVENET--RGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 125

Query: 122 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREG-----RIGGLEEALKILSLLS 181
           ++KL  LA++ +  +R  M E GV K ++LFV++   E      RI GL+E+L++L L+ 
Sbjct: 126 LQKLEVLAMDGN--NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 185

Query: 182 HYSISEIRFL----EFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLE 241
             S      L       ESLTWVL  E     + K+Y I +L+  T+   S I+ ++N E
Sbjct: 186 IPSNDAKTILMENDRVMESLTWVLHQE---DFLSKAYTIVLLRNLTEYTSSHIVERLNPE 245

Query: 242 FFKNITNLF--------------REKISNSS---------------------LKTTLTML 301
            FK I                  RE + +SS                     +   L +L
Sbjct: 246 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 305

Query: 302 INICPWGRNRVKLVEVGAVFDLIELELE-KPEKNITELIFNLLAHLCSTADGRAELISHA 361
           +    W RNR  LV++GAV +LIELE+    EK ITEL+  +L+ LC  A+GRAE+++H 
Sbjct: 306 LETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 365

Query: 362 GGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVGAVSKLCMIMQSNCEG 406
           GGIAVV+KR+LRVS A  DRAI IL+ +SK S    V+ EM+ VG V KLC ++  +C  
Sbjct: 366 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGL 425

BLAST of IVF0017688 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 3.7e-70
Identity = 162/409 (39.61%), Postives = 239/409 (58.44%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           EE+ +PP+F+CPISL+IMKDPV  +TGITYDR+SIE W    K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-SCPVTKQDITDA-D 67

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDAV 121
           LTPNHTLRRLI+SW   NAS   G +   TP P + K+ + KL+R+   AS    ++  +
Sbjct: 68  LTPNHTLRRLIQSWCTLNAS--YGVERIPTPRPPICKSEIEKLIRD--SASSHENQVKCL 127

Query: 122 KKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSISE 181
           K+L  +  E+ T  R  +E  GV + +   V      G +   +EA   L+LL H   SE
Sbjct: 128 KRLRQIVSENATNKR-CLEAAGVPEFLANIVSNDSENGSL--TDEA---LNLLYHLETSE 187

Query: 182 I---------RFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEF 241
                     +     +SLT ++   M    +   YA  +LK   +VA       +  E 
Sbjct: 188 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRV---YATLLLKNILEVADPMQSMTLKPEV 247

Query: 242 FKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEK---PEKN 301
           F  +  +  ++IS  + K  + +L+NICPWGRNR K VE G +  +IEL +++    E+ 
Sbjct: 248 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 307

Query: 302 ITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSAR 361
             E+   +L  LC  A+GRAE ++H   IAVV K+ILRVS   +DRA+++L  + +  A 
Sbjct: 308 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 367

Query: 362 KDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCI 399
             +L EML++G V+KLC+++Q +C G  KEKA+E+L++H+ VW +SPC+
Sbjct: 368 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of IVF0017688 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.5e-63
Identity = 154/430 (35.81%), Postives = 237/430 (55.12%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           +E+ +P +F+CPISL IMKDPV  +TGITYDRESIE W  + K    CP+TKQ +T+ +D
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-SCPVTKQVITE-TD 62

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDAV 121
           LTPNHTLRRLI+SW   NAS   G +   TP P + K+ + KL++E   +S    ++  +
Sbjct: 63  LTPNHTLRRLIQSWCTLNAS--YGIERIPTPKPPICKSEIEKLIKE--SSSSHLNQVKCL 122

Query: 122 KKLHALAIESDTASRGRMEEVGVAKGMVLFVIR-----------------------RFRE 181
           K+L  +  E+ T  R  +E   V + +   V                            E
Sbjct: 123 KRLRQIVSENTTNKR-CLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLE 182

Query: 182 GRIGGLEEAL-KILSLLSHYSISEIRFLEFFE-----SLTWVLGLEMENHTI-IKSYAIE 241
            R       + + LS+L H   SE             +L   L   M+      ++YA  
Sbjct: 183 NRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAAL 242

Query: 242 VLKKATDVAPSTILTQINLEFFKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVE 301
           +LKK  +VA    +  +  E F  +  +  ++IS+ + ++ + +L+  CPWGRNR K VE
Sbjct: 243 LLKKLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVE 302

Query: 302 VGAVFDLIELELE---KPEKNITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRV 361
            G +  +IEL ++     E+  +E+   +L  LC  A+GRAE ++H   IAVVSK+ILRV
Sbjct: 303 GGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRV 362

Query: 362 SIATTDRAIQILSLISKHSARKDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMH 399
           S  T++RA+++L  + +  A   +L EML++G V+KLC+++Q +C    KEKA+E+L++H
Sbjct: 363 SQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLH 422

BLAST of IVF0017688 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.6e-27
Identity = 116/401 (28.93%), Postives = 191/401 (47.63%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG TYDR SIE+W     +   CP+T+  L+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTT-CPVTRAPLSDFT-LI 71

Query: 64  PNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVAS----DERLRID 123
           PNHTLRRLI+ W V N S   G +   TP    D T +R LL + +  +      R R  
Sbjct: 72  PNHTLRRLIQEWCVANRS--NGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 131

Query: 124 AVKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLL----- 183
           A+++L   A +SD  +R  +      + ++  +        +  + E+L +L +L     
Sbjct: 132 ALRRLRGFARDSD-KNRVLIAAHNATEILIKILFSETTSSEL--VSESLALLVMLPITEP 191

Query: 184 -SHYSI-SEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEF 243
               SI S+   +EF   L +   +E     +  +  IE++   T  A        +   
Sbjct: 192 NQFVSISSDPGRVEFLTRLLFDSSIETR---VNAAALIEIVSTGTKSADLKGSISNSESV 251

Query: 244 FKNITNLFREKISN-SSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNIT 303
           F+ + +L R  IS+  +LK  +  L  +C     R   +  GA   LI+      ++  T
Sbjct: 252 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 311

Query: 304 ELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKD 363
           E     +  LC T +G A    HA  + ++ K ILRVS   T+ A    +L++  +A + 
Sbjct: 312 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG--ALLALCTAEER 371

Query: 364 VLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVW 393
              E    G V +L +++QS C    K+KA+++L++  + W
Sbjct: 372 WREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of IVF0017688 vs. ExPASy Swiss-Prot
Match: Q5PNY6 (U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.1e-24
Identity = 109/393 (27.74%), Postives = 190/393 (48.35%), Query Frame = 0

Query: 3   EVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASDL 62
           E+ +PP F CPIS+ +MKDPV  +TGITYDR SIE W  +      CP+T   LT   D 
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKT--CPVTNTVLT-TFDQ 87

Query: 63  TPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVAS---DERLRID 122
            PNHT+R++I+ W VE  SP        TP   L    + ++ R+++ A+   D      
Sbjct: 88  IPNHTIRKMIQGWCVEKGSP--LIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGV 147

Query: 123 AVKKLHALAIESDTASRGRMEEVGVAKGMVL---FVIRRFREGRIGGLEEALKILSLLSH 182
            ++K+  L  ES+  +R  + E  V  G VL   F      E     L E L +L+ +  
Sbjct: 148 IIEKIKKLGDESE-KNRKCVNENSV--GWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFP 207

Query: 183 YSISEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILT-QINLEFFKN 242
             +  I  L    S   V GL       ++  A  ++K+   +  + + +  +     + 
Sbjct: 208 IGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEA 267

Query: 243 ITNLFREKISNSSLKTTLTMLIN-ICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELI 302
           +  L R+ +S+SS K++L  +   +        + +E+G V   +E+ ++  E ++ E  
Sbjct: 268 LVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVD-AENSVCEKA 327

Query: 303 FNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLL 362
             +L  +C T  GR E+  +A  + ++ K+I +VS   T  ++ ++  + K +     + 
Sbjct: 328 LAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK-TGNTVAVE 387

Query: 363 EMLRVGAVSKLCMIMQSNCEGFLKEKAREILRM 388
           + +R+GA  K+ +++Q       KEKA E+L+M
Sbjct: 388 DAVRLGAFQKVLLVLQVGYGEETKEKATELLKM 410

BLAST of IVF0017688 vs. ExPASy TrEMBL
Match: A0A5A7TDP8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1933G00240 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.6e-225
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240
           EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300
           REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360
           LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

Query: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 409
           AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. ExPASy TrEMBL
Match: A0A1S3BP96 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492061 PE=4 SV=1)

HSP 1 Score: 790.0 bits (2039), Expect = 4.6e-225
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240
           EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300
           REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360
           LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

Query: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 409
           AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. ExPASy TrEMBL
Match: A0A0A0M309 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G633420 PE=4 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.3e-206
Identity = 380/409 (92.91%), Postives = 391/409 (95.60%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSK-DAIFCPLTKQSLTKA 60
           MEE AVPPYFICPISLQIMKDPVTATTGITYDRESI+NWFLTSK DAIFCPLTKQSLTKA
Sbjct: 1   MEEAAVPPYFICPISLQIMKDPVTATTGITYDRESIQNWFLTSKDDAIFCPLTKQSLTKA 60

Query: 61  SDLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRID 120
           SDLTPNHTLRRLIKSWMVENAS  GGDD+ LTP PLLDKT LRK+LREVA ASDERLRID
Sbjct: 61  SDLTPNHTLRRLIKSWMVENASSAGGDDQILTPRPLLDKTCLRKILREVA-ASDERLRID 120

Query: 121 AVKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSI 180
           +VKKLHALAIESDTA+RGRMEEVGVAKGMVLFVIRRFREGR+GGLEEALKILSLLSHYSI
Sbjct: 121 SVKKLHALAIESDTANRGRMEEVGVAKGMVLFVIRRFREGRVGGLEEALKILSLLSHYSI 180

Query: 181 SEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNL 240
           SE R L+FFESLTWVLGLEMENH IIKSYAIEVLKKATDVAPSTILTQIN+EFFKNITNL
Sbjct: 181 SETRVLDFFESLTWVLGLEMENHIIIKSYAIEVLKKATDVAPSTILTQINIEFFKNITNL 240

Query: 241 FREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLA 300
            REKISNSSLKTTLT+L NICP GRNRVKLVEVGAVFDLI LELEKPEK  TELIFNLLA
Sbjct: 241 LREKISNSSLKTTLTILTNICPCGRNRVKLVEVGAVFDLIALELEKPEKKTTELIFNLLA 300

Query: 301 HLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360
           HLCSTADGRAELI HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV
Sbjct: 301 HLCSTADGRAELIRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 409
           GAVSKLCM+MQSNCEG+LKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 GAVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. ExPASy TrEMBL
Match: A0A6J1HAU5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461696 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 6.1e-185
Identity = 336/416 (80.77%), Postives = 371/416 (89.18%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTL RLIKSW+VENA+PG G D  L+P PLLDKT LRKLLR++  ASD+ LR DA
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE LKILS+L  YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQI--------NLEF 240
           E R L+  +SL WVLGLEMENH IIKSYAIEVLKKA D APST+++          +L+F
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQF 240

Query: 241 FKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITE 300
           FKN+TNL REKISNS+LKTTLT+L  +C WGRNR+KLVEVGAVFDLI LELEKPEKNITE
Sbjct: 241 FKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITE 300

Query: 301 LIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360
           LIFNLLAHLC+ ADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV
Sbjct: 301 LIFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360

Query: 361 LLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 409
           L+EMLRVGAVSKLCM+MQS+CEG+LKEKAREILRMHSNVWSNSPCIGVY+MTRDPR
Sbjct: 361 LVEMLRVGAVSKLCMVMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYLMTRDPR 415

BLAST of IVF0017688 vs. ExPASy TrEMBL
Match: A0A6J1JGZ2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485014 PE=4 SV=1)

HSP 1 Score: 654.4 bits (1687), Expect = 3.0e-184
Identity = 337/423 (79.67%), Postives = 372/423 (87.94%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQ LTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTL RLIKSW+VENA+PG G D  L+P PLLDKT LRKLLR++  ASD+ LR DA
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDL-TASDDGLRHDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE LKILS+L  YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQI------------ 240
           E R L+  +SL WVLGLEMENH IIKSYAIEVLKKA D APST+++              
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYELRYHEFESRYQ 240

Query: 241 ---NLEFFKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEK 300
              +L+FFKN+TNL REKISNS+LKTTLT+L  +C WGRNR+KLVEVGAVF+LI LELEK
Sbjct: 241 GFESLQFFKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFNLIALELEK 300

Query: 301 PEKNITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISK 360
           PEKNITELIFNLLAHLC+TADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISK
Sbjct: 301 PEKNITELIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISK 360

Query: 361 HSARKDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTR 409
           HSARKDVL+EMLRVGAVSKLCM+MQS+CEG+LKEKAREILRMHSNVWSNSPCIGVY+MTR
Sbjct: 361 HSARKDVLVEMLRVGAVSKLCMMMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYLMTR 420

BLAST of IVF0017688 vs. NCBI nr
Match: XP_008450468.1 (PREDICTED: E3 ubiquitin-protein ligase PUB24-like [Cucumis melo] >KAA0040216.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa] >TYK07878.1 E3 ubiquitin-protein ligase PUB24-like [Cucumis melo var. makuwa])

HSP 1 Score: 786 bits (2029), Expect = 3.31e-286
Identity = 408/408 (100.00%), Postives = 408/408 (100.00%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA
Sbjct: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS
Sbjct: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240
           EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF
Sbjct: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240

Query: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300
           REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300

Query: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360
           LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG
Sbjct: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360

Query: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408
           AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. NCBI nr
Match: XP_004139143.1 (E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66591.1 hypothetical protein Csa_007018 [Cucumis sativus])

HSP 1 Score: 724 bits (1870), Expect = 5.61e-262
Identity = 380/409 (92.91%), Postives = 391/409 (95.60%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKD-AIFCPLTKQSLTKA 60
           MEE AVPPYFICPISLQIMKDPVTATTGITYDRESI+NWFLTSKD AIFCPLTKQSLTKA
Sbjct: 1   MEEAAVPPYFICPISLQIMKDPVTATTGITYDRESIQNWFLTSKDDAIFCPLTKQSLTKA 60

Query: 61  SDLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRID 120
           SDLTPNHTLRRLIKSWMVENAS  GGDD+ LTP PLLDKT LRK+LREVA ASDERLRID
Sbjct: 61  SDLTPNHTLRRLIKSWMVENASSAGGDDQILTPRPLLDKTCLRKILREVA-ASDERLRID 120

Query: 121 AVKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSI 180
           +VKKLHALAIESDTA+RGRMEEVGVAKGMVLFVIRRFREGR+GGLEEALKILSLLSHYSI
Sbjct: 121 SVKKLHALAIESDTANRGRMEEVGVAKGMVLFVIRRFREGRVGGLEEALKILSLLSHYSI 180

Query: 181 SEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNL 240
           SE R L+FFESLTWVLGLEMENH IIKSYAIEVLKKATDVAPSTILTQIN+EFFKNITNL
Sbjct: 181 SETRVLDFFESLTWVLGLEMENHIIIKSYAIEVLKKATDVAPSTILTQINIEFFKNITNL 240

Query: 241 FREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLA 300
            REKISNSSLKTTLT+L NICP GRNRVKLVEVGAVFDLI LELEKPEK  TELIFNLLA
Sbjct: 241 LREKISNSSLKTTLTILTNICPCGRNRVKLVEVGAVFDLIALELEKPEKKTTELIFNLLA 300

Query: 301 HLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360
           HLCSTADGRAELI HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV
Sbjct: 301 HLCSTADGRAELIRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRV 360

Query: 361 GAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408
           GAVSKLCM+MQSNCEG+LKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 GAVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. NCBI nr
Match: XP_038879919.1 (E3 ubiquitin-protein ligase PUB24-like [Benincasa hispida])

HSP 1 Score: 710 bits (1832), Expect = 3.45e-256
Identity = 364/408 (89.22%), Postives = 386/408 (94.61%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS
Sbjct: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTLRRLIKSW+VENA PGGG D+ L P P LDKTRLRKLL++++VASD RLRI+A
Sbjct: 61  DLTPNHTLRRLIKSWLVENAGPGGGVDQILNPKPPLDKTRLRKLLQDLSVASDNRLRIEA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           VKKL ALAIESDTA+RG MEEVGVAKGMVLFVIR FREGR+GGLEEAL ILSLLS+YSIS
Sbjct: 121 VKKLEALAIESDTANRGSMEEVGVAKGMVLFVIRCFREGRVGGLEEALNILSLLSNYSIS 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEFFKNITNLF 240
           E R ++FFESLTWVLGL+MENH IIKSYA+EVLKKATDVAPSTILTQINLE FKNITNL 
Sbjct: 181 ETRVVDFFESLTWVLGLKMENHIIIKSYAVEVLKKATDVAPSTILTQINLELFKNITNLL 240

Query: 241 REKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELIFNLLAH 300
           REKISNSSLKTTLT+L N+CPWG+NR+KLV+VG VFDLI LELEKP KNITELIFNLLAH
Sbjct: 241 REKISNSSLKTTLTILTNVCPWGQNRIKLVKVGTVFDLIALELEKPAKNITELIFNLLAH 300

Query: 301 LCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVG 360
           LCSTADGRAEL+ HAGGIAVVSKRILRVS ATTDRAIQILSLISKHSARKDVLLEMLRVG
Sbjct: 301 LCSTADGRAELLRHAGGIAVVSKRILRVSPATTDRAIQILSLISKHSARKDVLLEMLRVG 360

Query: 361 AVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408
           AVSKLCM+MQSNCEG+LKEKAREILRMHSNVWSNSPCIGVYVMTRDPR
Sbjct: 361 AVSKLCMMMQSNCEGYLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408

BLAST of IVF0017688 vs. NCBI nr
Match: XP_022961078.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita moschata] >KAG7023800.1 E3 ubiquitin-protein ligase PUB24, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652 bits (1683), Expect = 2.20e-233
Identity = 336/416 (80.77%), Postives = 371/416 (89.18%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTL RLIKSW+VENA+PG G D  L+P PLLDKT LRKLLR++  ASD+ LR DA
Sbjct: 61  DLTPNHTLHRLIKSWLVENATPGSGFDRILSPRPLLDKTHLRKLLRDLT-ASDDGLRHDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE LKILS+L  YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQI--------NLEF 240
           E R L+  +SL WVLGLEMENH IIKSYAIEVLKKA D APST+++          +L+F
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDAAPSTVISGYEPRYQGFESLQF 240

Query: 241 FKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITE 300
           FKN+TNL REKISNS+LKTTLT+L  +C WGRNR+KLVEVGAVFDLI LELEKPEKNITE
Sbjct: 241 FKNVTNLLREKISNSALKTTLTILTKLCHWGRNRIKLVEVGAVFDLIALELEKPEKNITE 300

Query: 301 LIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360
           LIFNLLAHLC+ ADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV
Sbjct: 301 LIFNLLAHLCTMADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360

Query: 361 LLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408
           L+EMLRVGAVSKLCM+MQS+CEG+LKEKAREILRMHSNVWSNSPCIGVY+MTRDPR
Sbjct: 361 LVEMLRVGAVSKLCMVMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYLMTRDPR 415

BLAST of IVF0017688 vs. NCBI nr
Match: XP_023516826.1 (E3 ubiquitin-protein ligase PUB24-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652 bits (1682), Expect = 3.12e-233
Identity = 335/416 (80.53%), Postives = 372/416 (89.42%), Query Frame = 0

Query: 1   MEEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKAS 60
           M+E+AVPPYFICPISLQIMKDPVTATTGITYDRESIENWF+TSKDAIFCPLTKQ LTKAS
Sbjct: 1   MDELAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFMTSKDAIFCPLTKQPLTKAS 60

Query: 61  DLTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDA 120
           DLTPNHTL RLIK W+VENA+PG G D  L+P PLLDKT LRKLLR++A ASD+ LR DA
Sbjct: 61  DLTPNHTLHRLIKCWLVENATPGSGFDGILSPRPLLDKTHLRKLLRDLA-ASDDGLRHDA 120

Query: 121 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSIS 180
           V+KL+ALAIE DTA RGRM EVGVAKGMVLFV+R F+EG++GGLEE LKILS+L  YSI 
Sbjct: 121 VRKLYALAIEGDTACRGRMVEVGVAKGMVLFVMRCFKEGKVGGLEEGLKILSVLESYSIC 180

Query: 181 EIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQI--------NLEF 240
           E R L+  +SL WVLGLEMENH IIKSYAIEVLKKA DVAPST+++          +L+F
Sbjct: 181 ETRVLDVIDSLNWVLGLEMENHIIIKSYAIEVLKKAIDVAPSTVISGYEPLCRGFESLQF 240

Query: 241 FKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNITE 300
           FKN+TN  R+KISNS+LKTTLT+L  +C WGRNR+KLVEVGA+FDLI LELEKPEKNITE
Sbjct: 241 FKNVTNFLRQKISNSALKTTLTILTKLCHWGRNRIKLVEVGAIFDLIALELEKPEKNITE 300

Query: 301 LIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360
           LIFNLLAHLC+TADGRAEL+ HAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV
Sbjct: 301 LIFNLLAHLCTTADGRAELVRHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDV 360

Query: 361 LLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCIGVYVMTRDPR 408
           L+EMLRVGAVSKLCM+MQS+CEG+LKEKAREILRMHSNVWSNSPCIGVY+MTRDPR
Sbjct: 361 LVEMLRVGAVSKLCMVMQSDCEGYLKEKAREILRMHSNVWSNSPCIGVYLMTRDPR 415

BLAST of IVF0017688 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 270.8 bits (691), Expect = 1.8e-72
Identity = 180/450 (40.00%), Postives = 251/450 (55.78%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           EE+ +P YFICPISL+IMKDPVT  +GITYDR++I  W    +    CP+TKQ L   SD
Sbjct: 20  EEIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWL---EKVPSCPVTKQPLPLDSD 79

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERL-RIDA 121
           LTPNH LRRLI+ W VEN +   G     TP     K  + + ++ +     E L R + 
Sbjct: 80  LTPNHMLRRLIQHWCVENET--RGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREET 139

Query: 122 VKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREG-----RIGGLEEALKILSLLS 181
           ++KL  LA++ +  +R  M E GV K ++LFV++   E      RI GL+E+L++L L+ 
Sbjct: 140 LQKLEVLAMDGN--NRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIG 199

Query: 182 HYSISEIRFL----EFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLE 241
             S      L       ESLTWVL  E     + K+Y I +L+  T+   S I+ ++N E
Sbjct: 200 IPSNDAKTILMENDRVMESLTWVLHQE---DFLSKAYTIVLLRNLTEYTSSHIVERLNPE 259

Query: 242 FFKNITNLF--------------REKISNSS---------------------LKTTLTML 301
            FK I                  RE + +SS                     +   L +L
Sbjct: 260 IFKGIIGFLKDVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMIL 319

Query: 302 INICPWGRNRVKLVEVGAVFDLIELELE-KPEKNITELIFNLLAHLCSTADGRAELISHA 361
           +    W RNR  LV++GAV +LIELE+    EK ITEL+  +L+ LC  A+GRAE+++H 
Sbjct: 320 LETSSWSRNRSLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHR 379

Query: 362 GGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLLEMLRVGAVSKLCMIMQSNCEG 406
           GGIAVV+KR+LRVS A  DRAI IL+ +SK S    V+ EM+ VG V KLC ++  +C  
Sbjct: 380 GGIAVVTKRLLRVSPAADDRAISILTTVSKFSPENMVVEEMVNVGTVEKLCSVLGMDCGL 439

BLAST of IVF0017688 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 266.9 bits (681), Expect = 2.6e-71
Identity = 162/409 (39.61%), Postives = 239/409 (58.44%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           EE+ +PP+F+CPISL+IMKDPV  +TGITYDR+SIE W    K    CP+TKQ +T A D
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKN-SCPVTKQDITDA-D 67

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDAV 121
           LTPNHTLRRLI+SW   NAS   G +   TP P + K+ + KL+R+   AS    ++  +
Sbjct: 68  LTPNHTLRRLIQSWCTLNAS--YGVERIPTPRPPICKSEIEKLIRD--SASSHENQVKCL 127

Query: 122 KKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLLSHYSISE 181
           K+L  +  E+ T  R  +E  GV + +   V      G +   +EA   L+LL H   SE
Sbjct: 128 KRLRQIVSENATNKR-CLEAAGVPEFLANIVSNDSENGSL--TDEA---LNLLYHLETSE 187

Query: 182 I---------RFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEF 241
                     +     +SLT ++   M    +   YA  +LK   +VA       +  E 
Sbjct: 188 TVLKNLLNNKKDNNIVKSLTKIMQRGMYESRV---YATLLLKNILEVADPMQSMTLKPEV 247

Query: 242 FKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEK---PEKN 301
           F  +  +  ++IS  + K  + +L+NICPWGRNR K VE G +  +IEL +++    E+ 
Sbjct: 248 FTEVVQILDDRISQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERR 307

Query: 302 ITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSAR 361
             E+   +L  LC  A+GRAE ++H   IAVV K+ILRVS   +DRA+++L  + +  A 
Sbjct: 308 GPEMAMVVLDLLCQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCAT 367

Query: 362 KDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVWSNSPCI 399
             +L EML++G V+KLC+++Q +C G  KEKA+E+L++H+ VW +SPC+
Sbjct: 368 PALLHEMLQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

BLAST of IVF0017688 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 245.0 bits (624), Expect = 1.1e-64
Identity = 154/430 (35.81%), Postives = 237/430 (55.12%), Query Frame = 0

Query: 2   EEVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASD 61
           +E+ +P +F+CPISL IMKDPV  +TGITYDRESIE W  + K    CP+TKQ +T+ +D
Sbjct: 3   QEIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKN-SCPVTKQVITE-TD 62

Query: 62  LTPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVASDERLRIDAV 121
           LTPNHTLRRLI+SW   NAS   G +   TP P + K+ + KL++E   +S    ++  +
Sbjct: 63  LTPNHTLRRLIQSWCTLNAS--YGIERIPTPKPPICKSEIEKLIKE--SSSSHLNQVKCL 122

Query: 122 KKLHALAIESDTASRGRMEEVGVAKGMVLFVIR-----------------------RFRE 181
           K+L  +  E+ T  R  +E   V + +   V                            E
Sbjct: 123 KRLRQIVSENTTNKR-CLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLE 182

Query: 182 GRIGGLEEAL-KILSLLSHYSISEIRFLEFFE-----SLTWVLGLEMENHTI-IKSYAIE 241
            R       + + LS+L H   SE             +L   L   M+      ++YA  
Sbjct: 183 NRFDSSRSLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQRGIYESRAYAAL 242

Query: 242 VLKKATDVAPSTILTQINLEFFKNITNLFREKISNSSLKTTLTMLINICPWGRNRVKLVE 301
           +LKK  +VA    +  +  E F  +  +  ++IS+ + ++ + +L+  CPWGRNR K VE
Sbjct: 243 LLKKLLEVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVE 302

Query: 302 VGAVFDLIELELE---KPEKNITELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRV 361
            G +  +IEL ++     E+  +E+   +L  LC  A+GRAE ++H   IAVVSK+ILRV
Sbjct: 303 GGTISMIIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRV 362

Query: 362 SIATTDRAIQILSLISKHSARKDVLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMH 399
           S  T++RA+++L  + +  A   +L EML++G V+KLC+++Q +C    KEKA+E+L++H
Sbjct: 363 SQITSERAVRVLLSVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLH 422

BLAST of IVF0017688 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 123.2 bits (308), Expect = 4.7e-28
Identity = 116/401 (28.93%), Postives = 191/401 (47.63%), Query Frame = 0

Query: 4   VAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASDLT 63
           + +P +F CPISL++M+DPVT  TG TYDR SIE+W     +   CP+T+  L+  + L 
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTT-CPVTRAPLSDFT-LI 71

Query: 64  PNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVAS----DERLRID 123
           PNHTLRRLI+ W V N S   G +   TP    D T +R LL + +  +      R R  
Sbjct: 72  PNHTLRRLIQEWCVANRS--NGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAA 131

Query: 124 AVKKLHALAIESDTASRGRMEEVGVAKGMVLFVIRRFREGRIGGLEEALKILSLL----- 183
           A+++L   A +SD  +R  +      + ++  +        +  + E+L +L +L     
Sbjct: 132 ALRRLRGFARDSD-KNRVLIAAHNATEILIKILFSETTSSEL--VSESLALLVMLPITEP 191

Query: 184 -SHYSI-SEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILTQINLEF 243
               SI S+   +EF   L +   +E     +  +  IE++   T  A        +   
Sbjct: 192 NQFVSISSDPGRVEFLTRLLFDSSIETR---VNAAALIEIVSTGTKSADLKGSISNSESV 251

Query: 244 FKNITNLFREKISN-SSLKTTLTMLINICPWGRNRVKLVEVGAVFDLIELELEKPEKNIT 303
           F+ + +L R  IS+  +LK  +  L  +C     R   +  GA   LI+      ++  T
Sbjct: 252 FEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDT 311

Query: 304 ELIFNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKD 363
           E     +  LC T +G A    HA  + ++ K ILRVS   T+ A    +L++  +A + 
Sbjct: 312 ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAG--ALLALCTAEER 371

Query: 364 VLLEMLRVGAVSKLCMIMQSNCEGFLKEKAREILRMHSNVW 393
              E    G V +L +++QS C    K+KA+++L++  + W
Sbjct: 372 WREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSW 400

BLAST of IVF0017688 vs. TAIR 10
Match: AT5G37490.1 (ARM repeat superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 2.2e-25
Identity = 109/393 (27.74%), Postives = 190/393 (48.35%), Query Frame = 0

Query: 3   EVAVPPYFICPISLQIMKDPVTATTGITYDRESIENWFLTSKDAIFCPLTKQSLTKASDL 62
           E+ +PP F CPIS+ +MKDPV  +TGITYDR SIE W  +      CP+T   LT   D 
Sbjct: 28  EITIPPEFQCPISIDLMKDPVIISTGITYDRVSIETWINSGNKT--CPVTNTVLT-TFDQ 87

Query: 63  TPNHTLRRLIKSWMVENASPGGGDDEFLTPSPLLDKTRLRKLLREVAVAS---DERLRID 122
            PNHT+R++I+ W VE  SP        TP   L    + ++ R+++ A+   D      
Sbjct: 88  IPNHTIRKMIQGWCVEKGSP--LIQRIPTPRVPLMPCEVYEISRKLSSATRRGDYEKCGV 147

Query: 123 AVKKLHALAIESDTASRGRMEEVGVAKGMVL---FVIRRFREGRIGGLEEALKILSLLSH 182
            ++K+  L  ES+  +R  + E  V  G VL   F      E     L E L +L+ +  
Sbjct: 148 IIEKIKKLGDESE-KNRKCVNENSV--GWVLCDCFDKFSGDEKLTFMLNEILSLLTWMFP 207

Query: 183 YSISEIRFLEFFESLTWVLGLEMENHTIIKSYAIEVLKKATDVAPSTILT-QINLEFFKN 242
             +  I  L    S   V GL       ++  A  ++K+   +  + + +  +     + 
Sbjct: 208 IGLEGISKLASATSFRCVAGLLKSTDDSVRQNAAFIMKEILSLDETRVHSFAVENGVAEA 267

Query: 243 ITNLFREKISNSSLKTTLTMLIN-ICPWGRNRVKLVEVGAVFDLIELELEKPEKNITELI 302
           +  L R+ +S+SS K++L  +   +        + +E+G V   +E+ ++  E ++ E  
Sbjct: 268 LVKLIRDSVSSSSTKSSLIAIYQMVLQKPEIASEFLEIGLVSITVEMIVD-AENSVCEKA 327

Query: 303 FNLLAHLCSTADGRAELISHAGGIAVVSKRILRVSIATTDRAIQILSLISKHSARKDVLL 362
             +L  +C T  GR E+  +A  + ++ K+I +VS   T  ++ ++  + K +     + 
Sbjct: 328 LAVLDAICETEHGREEVRKNALVMPLLVKKIAKVSELATRSSMSMILKLWK-TGNTVAVE 387

Query: 363 EMLRVGAVSKLCMIMQSNCEGFLKEKAREILRM 388
           + +R+GA  K+ +++Q       KEKA E+L+M
Sbjct: 388 DAVRLGAFQKVLLVLQVGYGEETKEKATELLKM 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SF152.6e-7140.00E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ... [more]
Q84TG33.7e-7039.61E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ... [more]
Q9SVC61.5e-6335.81E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ... [more]
Q9LT796.6e-2728.93U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q5PNY63.1e-2427.74U-box domain-containing protein 21 OS=Arabidopsis thaliana OX=3702 GN=PUB21 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7TDP84.6e-225100.00RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BP964.6e-225100.00RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492061 PE=4 ... [more]
A0A0A0M3091.3e-20692.91RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G633420 PE... [more]
A0A6J1HAU56.1e-18580.77RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461696... [more]
A0A6J1JGZ23.0e-18479.67RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485014 P... [more]
Match NameE-valueIdentityDescription
XP_008450468.13.31e-286100.00PREDICTED: E3 ubiquitin-protein ligase PUB24-like [Cucumis melo] >KAA0040216.1 E... [more]
XP_004139143.15.61e-26292.91E3 ubiquitin-protein ligase PUB23 [Cucumis sativus] >KGN66591.1 hypothetical pro... [more]
XP_038879919.13.45e-25689.22E3 ubiquitin-protein ligase PUB24-like [Benincasa hispida][more]
XP_022961078.12.20e-23380.77E3 ubiquitin-protein ligase PUB24-like [Cucurbita moschata] >KAG7023800.1 E3 ubi... [more]
XP_023516826.13.12e-23380.53E3 ubiquitin-protein ligase PUB24-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G11840.11.8e-7240.00plant U-box 24 [more]
AT2G35930.12.6e-7139.61plant U-box 23 [more]
AT3G52450.11.1e-6435.81plant U-box 22 [more]
AT3G19380.14.7e-2828.93plant U-box 25 [more]
AT5G37490.12.2e-2527.74ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 9..75
e-value: 8.8E-28
score: 108.3
IPR003613U box domainPFAMPF04564U-boxcoord: 6..79
e-value: 1.3E-18
score: 67.0
IPR003613U box domainPROSITEPS51698U_BOXcoord: 5..82
score: 32.262032
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 99..387
e-value: 2.5E-9
score: 38.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 1..83
e-value: 3.4E-26
score: 93.1
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 4..404
NoneNo IPR availablePANTHERPTHR22849:SF24E3 UBIQUITIN-PROTEIN LIGASE PUB24coord: 4..404
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 10..54
e-value: 3.0364E-18
score: 75.7331
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 4..80
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 111..392

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0017688.2IVF0017688.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity