IVF0017521 (gene) Melon (IVF77) v1

Overview


Name	IVF0017521
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	kunitz trypsin inhibitor 2
Location	chr12: 24122191 .. 24122829 (+)
RNA-Seq Expression	IVF0017521
Synteny	IVF0017521

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGA

mRNA sequence

ATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGA

Coding sequence (CDS)

ATGAAGAAATTTGCATTACTCAGTTTTCTTTTCATTGCCATTGTCATTGCCTCATCTGAGCTACGCTTCTGCAGAGCTGATGCCTCACCGGATGCCGTCCTCGACACCGATGGAAAGAAGCTCAGAGTCAGCAACAAGTACTACATCCTCCCAGCATTTGAAGGCAGCGGTGGTGGCGGTTTAGCCATCGGCAATATCCGAAAAGAATACGATAGATGTGGTATCAACGTCGTCCAGGAGCGTTATGAACAATCCGATGGTGATCCCACGACATTTTTACCAATAAACCCTAAAAAGGGTGTGGTTCGAGTCTCCACCGATTTGAACATCGAATTTGATGCGACTACGAGGTGTAGGAAATCGACGGTGTGGAAATTAGGAACATTTGATCGATATTTGAGGCAATATTTCGTGACGATTGGCGGAACGAAAGGGAATCCGGGGCGAGAGACGACAGAAAATTGGTTCAAAATAGAGAAATATGGGAAGGGTAATTACAAGTTGGTGTATTGTCCAAGAGTGTGTAAGTATTGCAAAGTAATGTGCAAAGATATTGGTATATTTGAGAACAATGGGATAAGGGGTCTTGTTTTGAGCGATACGCCATTTCCCGTTATCTTCAAGAAAGTTTATGAATGA

Protein sequence

MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE

Homology

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BLAST of IVF0017521 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 189.9 bits (481), Expect = 3.0e-47
Identity = 103/208 (49.52%), Postives = 123/208 (59.13%), Query Frame = 0


Query: 7   LSFLFI-AIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 66
           LSF F+ A++ A++      AD++P+ VLD DG+KLR    YYI+P     GGG      
Sbjct: 9   LSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 68

Query: 67  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR--KSTV 126
                  C   VVQ R E     P  F P NPK+ VVRVSTDLNI F A   CR   STV
Sbjct: 69  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 128

Query: 127 WKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKY-GKGNYKLVYCPRVCKYCKVMC 186
           W+L  +D    QYFVTIGG KGNPG ET  +WFKIE++ G G YKLV+CP VC  CKV C
Sbjct: 129 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTVCGSCKVKC 188

Query: 187 KDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
            D+GI+ +  G R L LSD PF   F K
Sbjct: 189 GDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of IVF0017521 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 7.1e-41
Identity = 85/206 (41.26%), Postives = 128/206 (62.14%), Query Frame = 0


Query: 6   LLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 65
           L  FL +A+ I+    R    +A+ + V D +GK L     YYILP   G  GGGL + N
Sbjct: 5   LYIFLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSN 64

Query: 66  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWK 125
           ++ E   C  +V+Q+++E S G P  F P + K   + VSTD+NI+F  T      ++W+
Sbjct: 65  LKTE--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPT------SIWE 124

Query: 126 LGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDI 185
           L  FD   +Q+F++  G +GNPG++T +NWFKI+K+ K +YK+ +CP VC +CKV+C+D+
Sbjct: 125 LANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK-DYKIRFCPTVCNFCKVICRDV 184

Query: 186 GIFENNGIRGLVLSDTPFPVIFKKVY 212
           G+F  +G R L LSD P  V+FK+ Y
Sbjct: 185 GVFVQDGKRRLALSDVPLKVMFKRAY 196

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BLAST of IVF0017521 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.4e-35
Identity = 84/203 (41.38%), Postives = 112/203 (55.17%), Query Frame = 0


Query: 10  LFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKE 69
           + +     S    F  A+A+   VLDTDG +L+   +YY+L +  G+GGGGLA+G  R  
Sbjct: 8   VLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALG--RAT 67

Query: 70  YDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEF--DATTRCRKSTVWKLG 129
              C   VVQ R +  +G P  F   + K  VVRVSTD+NIEF       C  STVW+L 
Sbjct: 68  GQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 127

Query: 130 TFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGI 189
            +D    +++VT  G KG PG  T  +WFKIEK G   YK  +CP VC  C  +C DIG 
Sbjct: 128 NYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTTLCSDIGR 187

Query: 190 F-ENNGIRGLVLSDTPFPVIFKK 210
             +++G   L LSD  +  +FKK
Sbjct: 188 HSDDDGQIRLALSDNEWAWMFKK 208

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BLAST of IVF0017521 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.3e-29
Identity = 74/180 (41.11%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 32  AVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTT 91
           AV+D DG  +     YY+LP   G GGG    G   +    C  ++VQE  E  +G P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D   T C +ST W++G FD   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
           + +++FKIEK G+  YK V+CPR C      C D+GIF +  G+R L LSD PF V+FKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

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BLAST of IVF0017521 vs. ExPASy Swiss-Prot
Match: P80691 (Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 5.1e-23
Identity = 65/187 (34.76%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 29  SPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAI---GNIRKEYDRCGINVVQERYEQS 88
           +P  ++D DGK +     Y+++ A  G+GGGGL +   GN +K    C ++VVQ+ ++  
Sbjct: 2   APKPIVDIDGKPVLYGVDYFVVSAIWGAGGGGLTVYGPGNKKK----CPLSVVQDPFD-- 61

Query: 89  DGDPTTFLPI-NPKKGVVRVSTDLNIEFDATTRCRKSTVWKLGTFDRYLRQYFVTIGGTK 148
           +G+P  F  I N K  +V  S DLN++F+ T  C ++T WK+  F   +  + VT+GG K
Sbjct: 62  NGEPIIFSAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVI-GWTVTLGGEK 121

Query: 149 GNPGRETTENWFKIEKYG-KGNYKLVYCPRVCKYCKVMCKDIGI-FENNGIRGLVLSDTP 208
           G  G E+T + FKI+K G   +YK  +CP   +   + C ++ I F+   IR L+L++  
Sbjct: 122 GYHGFESTHSMFKIKKAGLPFSYKFHFCPSYPRTRLIPCNNVDIFFDKYRIRRLILTNDA 181

Query: 209 FPVIFKK 210
              +F K
Sbjct: 182 KEFVFIK 181

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BLAST of IVF0017521 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 5.5e-121
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 213
           MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212

[Close]

BLAST of IVF0017521 vs. ExPASy TrEMBL
Match: A0A1S3AT47 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 5.5e-121
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 213
           MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212

[Close]

BLAST of IVF0017521 vs. ExPASy TrEMBL
Match: A0A5D3BJ02 (Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.6e-78
Identity = 147/210 (70.00%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+A+TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVT+GGTKGNPGRET  NWFK+EK+GK NYK VYCP VCKYCKV
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGK-NYKFVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           MCKD+G+F  NG R   L+D PFPV+FKKV
Sbjct: 181 MCKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of IVF0017521 vs. ExPASy TrEMBL
Match: A0A1S3AST3 (miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 2.6e-78
Identity = 147/210 (70.00%), Postives = 170/210 (80.95%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MKNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+A+TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVT+GGTKGNPGRET  NWFK+EK+GK NYK VYCP VCKYCKV
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGK-NYKFVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           MCKD+G+F  NG R   L+D PFPV+FKKV
Sbjct: 181 MCKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of IVF0017521 vs. ExPASy TrEMBL
Match: A0A0A0LT81 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 9.8e-78
Identity = 146/210 (69.52%), Postives = 169/210 (80.48%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           M+ FALL FLF  IVIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +     GG
Sbjct: 1   MRNFALLCFLF--IVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVY-SRNSGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+   G P TF PINPKKGVVRVSTDLNI+F+A TRC  
Sbjct: 61  LSIGGI-YGYEKCPINILPESYDYLHGLPATFSPINPKKGVVRVSTDLNIQFEANTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD YL+QYFVT+GG KGNPGRET ENWFK+EKYGK NYKLVYCP VCKYCKV
Sbjct: 121 STVWKVGKFDEYLKQYFVTMGGMKGNPGRETIENWFKVEKYGK-NYKLVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 211
           +CKD+G+F  NG R + L+D PFPV+FKKV
Sbjct: 181 VCKDVGLFYKNGRRVIALNDAPFPVMFKKV 205

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BLAST of IVF0017521 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 441 bits (1135), Expect = 1.57e-156
Identity = 212/212 (100.00%), Postives = 212/212 (100.00%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212
           MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212

[Close]

BLAST of IVF0017521 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 301 bits (772), Expect = 2.65e-101
Identity = 147/210 (70.00%), Postives = 173/210 (82.38%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALLSFLFI IV  SSE+RFCRAD SPDAVLDTDGKKLR S++YYIL  +  +  GG
Sbjct: 1   MKNFALLSFLFIVIV--SSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNS-GG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG+I++  ++C IN++ E YE   G   TF PINPKKGVVRVSTDLNI+F+A+TRC +
Sbjct: 61  LSIGSIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGE 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVTIGG KGNPGRET  NWFKIEKYG  NYKLV+CP VCKYCKV
Sbjct: 121 STVWKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGN-NYKLVHCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210
           +CKD+GIF  NG R L L+D+PFPV+FKKV
Sbjct: 181 ICKDVGIFYKNGKRVLALNDSPFPVMFKKV 206

[Close]

BLAST of IVF0017521 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 300 bits (769), Expect = 7.84e-101
Identity = 147/210 (70.00%), Postives = 171/210 (81.43%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLFI  VIASSE+RFCRADASPDAVLDTDGKKLR  ++YYIL  +  +  GG
Sbjct: 1   MKNFALLCFLFI--VIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNS-GG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L+IG I   Y++C IN++ E Y+  DG P TF P+NPKKGVVRVSTDLNIEF+A+TRC  
Sbjct: 61  LSIGGIYG-YEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGI 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWK+G FD+YL+QYFVT+GGTKGNPGRET  NWFK+EK+GK NYK VYCP VCKYCKV
Sbjct: 121 STVWKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGK-NYKFVYCPTVCKYCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210
           MCKD+G+F  NG R   L+D PFPV+FKKV
Sbjct: 181 MCKDVGLFYKNGRRIFALNDAPFPVMFKKV 205

[Close]

BLAST of IVF0017521 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 282 bits (722), Expect = 1.01e-93
Identity = 138/210 (65.71%), Postives = 166/210 (79.05%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK FALL FLF   +IAS+E+R C++DASPDAVLD DGKKLR    YYILP   G G GG
Sbjct: 1   MKNFALLCFLFA--LIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRG-GG 60

Query: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120
           L +GNI   +D+C ++VVQE++E S+G P TF P+NPKKGVVRVSTDLN+EF+A+T C K
Sbjct: 61  LTLGNIG--HDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAK 120

Query: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180
           STVWKL  FD   RQ+ V IGG +GNPG+ET +NWFKIEK+GK +YK V+CP VCK+CKV
Sbjct: 121 STVWKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGK-DYKFVFCPSVCKFCKV 180

Query: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210
           +CKD+GIF  NG R L LSDTPFPV+FKKV
Sbjct: 181 ICKDVGIFIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of IVF0017521 vs. NCBI nr
Match: XP_038893656.1 (miraculin-like [Benincasa hispida])

HSP 1 Score: 278 bits (712), Expect = 4.10e-92
Identity = 136/214 (63.55%), Postives = 163/214 (76.17%), Query Frame = 0


Query: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60
           MK F LLS +FI IV ++   +FCR +ASP+A+LDT+GKKLR  + YYILP F  +  GG
Sbjct: 1   MKSFELLSLIFIVIVASTERQQFCRVNASPEAILDTNGKKLRGGDHYYILPFFSRNT-GG 60

Query: 61  LAIGNIRKEY-DRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCR 120
           LA+GNI++EY + C +NVV E YE  +G PTTF P+NPKKGV+RVSTDLNI+F+  T C 
Sbjct: 61  LALGNIQREYSNECPLNVVPEPYEYFNGLPTTFSPVNPKKGVIRVSTDLNIQFERNTMCA 120

Query: 121 KSTVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGN-YKLVYCPRVCKYC 180
           KSTVWK+  F++   QY VTIGGTKG+PG ET ENWFKIEKY   N YK VYCP VCKYC
Sbjct: 121 KSTVWKISKFEQ---QYLVTIGGTKGDPGPETMENWFKIEKYNNNNIYKFVYCPTVCKYC 180

Query: 181 KVMCKDIGIFENNGIRGLVLSDTPFPVIFKKVYE 212
           KVMCKD+GIF  NG   L LSD PFPV+FKKVY+
Sbjct: 181 KVMCKDVGIFVKNGRMALALSDAPFPVMFKKVYK 210

[Close]

BLAST of IVF0017521 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 168.7 bits (426), Expect = 5.0e-42
Identity = 85/206 (41.26%), Postives = 128/206 (62.14%), Query Frame = 0


Query: 6   LLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGN 65
           L  FL +A+ I+    R    +A+ + V D +GK L     YYILP   G  GGGL + N
Sbjct: 5   LYIFLLLAVFISH---RGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGR-GGGLTMSN 64

Query: 66  IRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRKSTVWK 125
           ++ E   C  +V+Q+++E S G P  F P + K   + VSTD+NI+F  T      ++W+
Sbjct: 65  LKTE--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPT------SIWE 124

Query: 126 LGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDI 185
           L  FD   +Q+F++  G +GNPG++T +NWFKI+K+ K +YK+ +CP VC +CKV+C+D+
Sbjct: 125 LANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK-DYKIRFCPTVCNFCKVICRDV 184

Query: 186 GIFENNGIRGLVLSDTPFPVIFKKVY 212
           G+F  +G R L LSD P  V+FK+ Y
Sbjct: 185 GVFVQDGKRRLALSDVPLKVMFKRAY 196

[Close]

BLAST of IVF0017521 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 131.3 bits (329), Expect = 8.9e-31
Identity = 74/180 (41.11%), Postives = 98/180 (54.44%), Query Frame = 0


Query: 32  AVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIGNIRKEYDRCGINVVQERYEQSDGDPTT 91
           AV+D DG  +     YY+LP   G GGG    G   +    C  ++VQE  E  +G P  
Sbjct: 29  AVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG---RGGQPCPYDIVQESSEVDEGIPVK 88

Query: 92  FLPINPKKGVVRVSTDLNIEFD-ATTRCRKSTVWKLGTFDRYLRQYFVTIGGTKGNPGRE 151
           F     K   V  S +LNIE D   T C +ST W++G FD   +QYFV  G      G++
Sbjct: 89  FSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQD 148

Query: 152 TTENWFKIEKYGKGNYKLVYCPRVCKYCKVMCKDIGIF-ENNGIRGLVLSDTPFPVIFKK 210
           + +++FKIEK G+  YK V+CPR C      C D+GIF +  G+R L LSD PF V+FKK
Sbjct: 149 SLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKK 204

[Close]

BLAST of IVF0017521 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 106.7 bits (265), Expect = 2.4e-23
Identity = 77/212 (36.32%), Postives = 115/212 (54.25%), Query Frame = 0


Query: 7   LSFLFIAIVIASSELRFC-RADASP-DAVLDTDGKKLRVSNKYYILPAFEGSGGGGLAIG 66
           L+  FI + + S+       ADA+P   VLD  G  ++ + +YYI+PA  G+GGG +   
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 67  NIRKEYDRC-GINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATT-RCRKST 126
                 D C  +++VQ       G P TF P+N K   V++S  LN+EFD+T   C  S 
Sbjct: 64  RNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSK 123

Query: 127 VWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKVMC 186
           VW++      LR+ FV+IGG KG        +WF+I++ G   YKL+YCP       V C
Sbjct: 124 VWRI-DHSVQLRKSFVSIGGQKGK-----GNSWFQIQEDGDA-YKLMYCP---ISSIVAC 183

Query: 187 KDIGI-FENNGIRGLVLS-DTPFPVIFKKVYE 213
            ++ +  +++G+R LVLS D  F V F+K Y+
Sbjct: 184 INVSLEIDDHGVRRLVLSTDQSFVVKFQKAYD 205

The following BLAST results are available for this feature:

BLAST of IVF0017521 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P13087	3.0e-47	49.52	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
Q9LMU2	7.1e-41	41.26	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P32765	3.4e-35	41.38	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.3e-29	41.11	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P80691	5.1e-23	34.76	Latex serine proteinase inhibitor OS=Carica papaya OX=3649 PE=1 SV=1	[more]

BLAST of IVF0017521 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BJ25	5.5e-121	100.00	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]
A0A1S3AT47	5.5e-121	100.00	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1	[more]
A0A5D3BJ02	2.6e-78	70.00	Miraculin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002800 PE=...	[more]
A0A1S3AST3	2.6e-78	70.00	miraculin OS=Cucumis melo OX=3656 GN=LOC103482610 PE=3 SV=1	[more]
A0A0A0LT81	9.8e-78	69.52	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043220 PE=3 SV=1	[more]

BLAST of IVF0017521 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008437071.1	1.57e-156	100.00	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]
XP_038895396.1	2.65e-101	70.00	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]
XP_008437082.1	7.84e-101	70.00	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_022137472.1	1.01e-93	65.71	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_038893656.1	4.10e-92	63.55	miraculin-like [Benincasa hispida]	[more]

BLAST of IVF0017521 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	5.0e-42	41.26	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	8.9e-31	41.11	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	2.4e-23	36.32	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 155..174 score: 28.73 coord: 73..93 score: 28.38 coord: 179..208 score: 23.66 coord: 32..61 score: 41.96
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 32..211 e-value: 2.1E-67 score: 239.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 33..209 e-value: 4.5E-53 score: 179.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..210
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 33..49
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 32..209 e-value: 1.03027E-53 score: 167.904
None	No IPR available	GENE3D	2.80.10.50		coord: 31..212 e-value: 1.8E-67 score: 228.5
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ TRYPSIN INHIBITOR 2	coord: 4..210
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 29..210

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0017521.2	IVF0017521.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity