IVF0016389 (gene) Melon (IVF77) v1

Overview
NameIVF0016389
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionubiquitin-conjugating enzyme E2 4-like
Locationtig00000267: 214667 .. 217364 (+)
RNA-Seq ExpressionIVF0016389
SyntenyIVF0016389
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTTCTCCAAGCAAACGCCGAGAGATGGACTTGATGAAACTGTTAATTGTCTAACAATAAGCTTATTCTAATTCCATGATCTCTATTTTTTTTTTTTGGGGGGGGGGGGGGGGGGGGGGGGGGTGCGTTTGTTAAGTTGTTCTGTGAAATCTTTGTTGATGGGGTTTTTTTTCTGTTCTTTTTTTCTTTTTTTGGGTTTGACAGGATGATGAGTGATTACAAGGGGAGATGATTAATGATGGAGTGCAAGAGTTTTATGTAGATTTTCATGGACCCAGTGAAAGTAAATCTTCAAATTTTGTAATTTATATGCTTTTTTGCTTGTTTCTTGAAGTTTGTATCTTGTTGAAATCTGGATGTGATCTACTTGTTCTCACCCAACATGAGCAATTATATGGTCATCTCGTTGAGTAATCCCCCTTTAGTTACTTTAAGTGGATTGTGATCTCAATAAAGTGAGAATCCTTCTGGGTTCATAGCAGCAACTGATTTTTTATGTGCTCATGTTGTGTTTGGCCATGCTGTTTTGAGGCACCTAACTTTCCCATGTTCTTGGAGAACAGAAGGAAATTGATAAAGAAAAGTCACAATGTGATGAGTTTCTGGCAGGTCCTTATCATGGAGGGTTATGGAGGATAAGAGTCGAACTTCCGGACGCTTACCCCTATAAATCTCCTTCAATAGGATTTGTAAACAAAATTTATCACCCCATGTTGATGAGATGTGAGTTCAACATATGTTCTTAACTTGAAGTGAGTTGTAGGTTTATGCTCTGATTATATGGTTTTCTGGTTGTCTTCAATACAGGTCGGGTTCGGTTTGTTTAGACGTTATTAATCAAACTTGGAGTCCCATGTTCGGTAGGCGAGCTTTTCTAATTGTTTATTATTCATCTTCATGCCATAGTTTTCAGATTGATCGTACATTTGTATATGCAATTACTTTCAGATCTGGTAAATGTTTTTGAAGTGTTCCTACCACAACTTCTTTTGTATCCCAACCGTCAGATCCTTTGAATGGAGAGGCTGCAGCCTTGATGATGCGTGATCGAGTTGCTTATGAACAAAAAGTAAAAGGTATTGTTTTTCTCAATGTTTCTGATTAGAAGCAAATCTATTGGAAGTGTGCTTTACAAGTGATTTTCTTGTTGTTTGCTGTCCATGTCATGACTATACATTTATTTGAAAGTTGGGTGTTTAAAGTTTAAAACTAATCGTCTATGGTTATTAGTGTGTTAACTTTTTATAAACATCAAATATTCAAAAATATAGTTTTTTCCCCATTGGAAATGCTGTTCTGTAAATTTGTAGAAATCCGGACAGGTTCATGCGATGCCTGAAAGCATTGCAGAAAATAAAGAGAGTTCTACGTTTGGGGATTATCTTTAGTGATTACTTCAACTTGACTTAAAGTTCAAATACTTGAGTACGATTTCCTCAGGGATATCCAATATAATTGGAACAATAAAGAGAAGATTAGCATGTCCCTACACAAGTATGACACACATCTACCAAGTGCTCGTTATATTTTCAAGTGGATTACTGTTAACTTGAAAAACCTGCTTTGGTTCCATCAGAATGTTACTATCAAGCCAATCTTCTTGCTCTATCATACATTTTCTTCCATCACTAGAAGATGTTCTTTTATCTGGCTTCATCTTTATACACTTTGAGATAGAATACTTGTGCGTTGTGTGTATGATGTTTGTTCTCCTATCCTACTAGCCGCCGTTACTAAGCCTGTAGGTGGTCCCTAGGCTCATGACTCTCCAGAAGTGCTCTATTGTCATTTCGTGTGATCTTTCTTCCATAGTAAGTATGGTAGTATCATGATCATTTGAATGTTTTGGGAAAGAGTGGAAGGTAATGATTTTTTCCCCAAGTGGACATTGAGTATTTTTGTCTATAACCTTTCTTTTTGTACGAGTAGTCTTTCATGAAAGAATAACAGTTACAAAGGGATGAAAGGAGAAAGACAAGAGTTACTCTCTTGATAAAAAGACTCTCGAATTGATTGAGAAATTCTCTTGATAAAAGCTCTGAGCGAAGTAAACAAAACTCAGACAACGGCCTGATTTTCCTTATCCCCTCTAATTAAATACTCTACCACTGTCATTCAAGTTATTTGATCAGTATTAAGGTGGAGGAAGATCGAAAAATGGTGATGATAACGATATATGCTTTGGTTATGGTGTCGCTGGGCAACAGTAGCATCATCTTTAGCAGTGGCAGCATAATAGCATCAATTCTAGAGCAAAATTTGTTAGTTTTTGGAGTTTAAGTTCCTCCACCAGAACTGGGTGGAATCTTTTTGTATTCGAGATTTTCAGGGTTCTATTTCTTCTGGAGGTTTTTATTCTGATGTCTGATATTAACTAGATAGTGCCCCAGTCGAGTATCTGTTACTCTTGCAGAATAGTTGGTTCAACGGCAAATGAAACATGTCCACGCAGTCATTACTTTTTTGATTGATGAAATCCTTATTGATCTTCTTTCTTGCTTTTTTAACCTTGTGAATTTCATTGAATTTGACCATTGTGGCTATTCTTCCATCTCATCTTACAGAATACTGTGAAAGATATGCAAAGCCAGAAGACATAGTAGCTGCACCTGATAAATCTAGCGACGAAGAAGAACTAAGTGAAGATGAATCCGACTTGAGCGATGAGCAAGTCATGGGGCAAGCAGACTTGTAA

mRNA sequence

ATGTCTTCTCCAAGCAAACGCCGAGAGATGGACTTGATGAAACTGTTAATTATGATTAATGATGGAGTGCAAGAGTTTTATGTAGATTTTCATGGACCCAGTGAAAGTCCTTATCATGGAGGGTTATGGAGGATAAGAGTCGAACTTCCGGACGCTTACCCCTATAAATCTCCTTCAATAGGATTTGTAAACAAAATTTATCACCCCATGTCGGGTTCGGTTTGTTTAGACGTTATTAATCAAACTTGGAGTCCCATTGTTCCTACCACAACTTCTTTTGTATCCCAACCGTCAGATCCTTTGAATGGAGAGGCTGCAGCCTTGATGATGCGTGATCGAGTTGCTTATGAACAAAAAGTAAAAGAATACTGTGAAAGATATGCAAAGCCAGAAGACATAGTAGCTGCACCTGATAAATCTAGCGACGAAGAAGAACTAAGTGAAGATGAATCCGACTTGAGCGATGAGCAAGTCATGGGGCAAGCAGACTTGTAA

Coding sequence (CDS)

ATGTCTTCTCCAAGCAAACGCCGAGAGATGGACTTGATGAAACTGTTAATTATGATTAATGATGGAGTGCAAGAGTTTTATGTAGATTTTCATGGACCCAGTGAAAGTCCTTATCATGGAGGGTTATGGAGGATAAGAGTCGAACTTCCGGACGCTTACCCCTATAAATCTCCTTCAATAGGATTTGTAAACAAAATTTATCACCCCATGTCGGGTTCGGTTTGTTTAGACGTTATTAATCAAACTTGGAGTCCCATTGTTCCTACCACAACTTCTTTTGTATCCCAACCGTCAGATCCTTTGAATGGAGAGGCTGCAGCCTTGATGATGCGTGATCGAGTTGCTTATGAACAAAAAGTAAAAGAATACTGTGAAAGATATGCAAAGCCAGAAGACATAGTAGCTGCACCTGATAAATCTAGCGACGAAGAAGAACTAAGTGAAGATGAATCCGACTTGAGCGATGAGCAAGTCATGGGGCAAGCAGACTTGTAA

Protein sequence

MSSPSKRREMDLMKLLIMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHPMSGSVCLDVINQTWSPIVPTTTSFVSQPSDPLNGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL
Homology
BLAST of IVF0016389 vs. ExPASy Swiss-Prot
Match: P16577 (Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 3.0e-55
Identity = 115/183 (62.84%), Postives = 132/183 (72.13%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDG+ EF+V FHGP +S Y GG+W++RVEL +AYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVDMINDGMHEFFVHFHGPKDSIYQGGVWKVRVELTEAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGF NKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFTNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNIFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDES-DLSDEQVMG 164
           NGEAA+LMMRD+ AYE KVKEYCERYAKPEDI    ++   +EELS+ E  D  DE +MG
Sbjct: 121 NGEAASLMMRDKNAYENKVKEYCERYAKPEDISPEEEEEESDEELSDAEGYDSGDEAIMG 180

BLAST of IVF0016389 vs. ExPASy Swiss-Prot
Match: P42748 (Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 SV=2)

HSP 1 Score: 208.8 bits (530), Expect = 4.8e-53
Identity = 116/177 (65.54%), Postives = 137/177 (77.40%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMD+MKL++       INDG+QEFYV+F+GP +S Y GG+W+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GF+ KIYHP    +SGSVCLDVINQTWSP   +V    +F+ Q      PSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDE--SDLSDE 157
           NGEAAALMMRDR AYEQ+VKEYCE+YAKP +   + DKSSD EELSE+E  SD  D+
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKPGE--GSEDKSSD-EELSEEEYGSDNEDD 174

BLAST of IVF0016389 vs. ExPASy Swiss-Prot
Match: P42749 (Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 SV=2)

HSP 1 Score: 207.6 bits (527), Expect = 1.1e-52
Identity = 114/177 (64.41%), Postives = 134/177 (75.71%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDG+QEF+V+F GP +S Y GG+W+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GF+ KIYHP    MSGSVCLDVINQTWSP   +V    +F+ Q      PSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDE--SDLSDE 157
           NGEAAALMMRDR  YEQ+VKEYCE+YAKP    A  ++ S ++E+SEDE  SD  DE
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDE 174

BLAST of IVF0016389 vs. ExPASy Swiss-Prot
Match: P42750 (Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 6.9e-52
Identity = 105/182 (57.69%), Postives = 133/182 (73.08%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           M+SPSKRREMD+MKL++       +ND +Q FYV FHGP++S Y GG+W+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GFVNKIYHP     SG+VCLDVINQTWSP   ++    SF+ Q      PSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAA+L+MRDR AYE KVKEYCE+YAKPE+I++  D      E   D  D  D++++G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

BLAST of IVF0016389 vs. ExPASy Swiss-Prot
Match: Q9P7R4 (Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=ubc8 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.4e-41
Identity = 90/175 (51.43%), Postives = 117/175 (66.86%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSP +R E D+MKLL+      ++ND +QEFYV FHGPSE+PY GG+W++ VELP  YP
Sbjct: 1   MSSPRRRIETDVMKLLMSDYEVTLVNDNMQEFYVRFHGPSETPYSGGIWKVHVELPSEYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           +KSPSIGFVN+I+HP    +SGSVCLDVINQTWSP+      F             SDPL
Sbjct: 61  WKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNASDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDE 157
           NGEAAAL++R+   Y  KV++Y  RYA  ED     + SSD++ +S  +++  DE
Sbjct: 121 NGEAAALLLREPNTYYAKVRDYIARYANKEDADITLNDSSDDDTMSSIDTESGDE 175

BLAST of IVF0016389 vs. ExPASy TrEMBL
Match: A0A1S3AW08 (ubiquitin-conjugating enzyme E2-23 kDa isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483264 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 1.8e-74
Identity = 152/183 (83.06%), Postives = 155/183 (84.70%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGFVNKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 165
           NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ
Sbjct: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 180

BLAST of IVF0016389 vs. ExPASy TrEMBL
Match: A0A1S3AVZ6 (ubiquitin-conjugating enzyme E2-23 kDa isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483264 PE=3 SV=1)

HSP 1 Score: 284.3 bits (726), Expect = 3.3e-73
Identity = 149/174 (85.63%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSFVSQPSDPLNGEAAALMM 120
           YKSPSIGFVNKIYHP    MSGSVCLDVINQTWSP+            DPLNGEAAALMM
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMF-----------DPLNGEAAALMM 120

Query: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 165
           RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL
Sbjct: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 163

BLAST of IVF0016389 vs. ExPASy TrEMBL
Match: A0A5D3D107 (AT-rich interactive domain-containing protein 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004770 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 9.3e-68
Identity = 137/160 (85.62%), Postives = 138/160 (86.25%), Query Frame = 0

Query: 18  MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP----MSGS 77
           MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP    MSGS
Sbjct: 557 MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHPNVDEMSGS 616

Query: 78  VCLDVINQTWSPIVPTTTSF---------VSQPSDPLNGEAAALMMRDRVAYEQKVKEYC 137
           VCLDVINQTWSP+      F            PSDPLNGEAAALMMRDRVAYEQKVKEYC
Sbjct: 617 VCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALMMRDRVAYEQKVKEYC 676

Query: 138 ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 165
           ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL
Sbjct: 677 ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 716

BLAST of IVF0016389 vs. ExPASy TrEMBL
Match: A0A5A7U0N0 (AT-rich interactive domain-containing protein 1-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001810 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 9.3e-68
Identity = 137/160 (85.62%), Postives = 138/160 (86.25%), Query Frame = 0

Query: 18  MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP----MSGS 77
           MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP    MSGS
Sbjct: 552 MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHPNVDEMSGS 611

Query: 78  VCLDVINQTWSPIVPTTTSF---------VSQPSDPLNGEAAALMMRDRVAYEQKVKEYC 137
           VCLDVINQTWSP+      F            PSDPLNGEAAALMMRDRVAYEQKVKEYC
Sbjct: 612 VCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPLNGEAAALMMRDRVAYEQKVKEYC 671

Query: 138 ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 165
           ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL
Sbjct: 672 ERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 711

BLAST of IVF0016389 vs. ExPASy TrEMBL
Match: A0A6J1D120 (ubiquitin-conjugating enzyme E2 4 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016307 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 2.1e-67
Identity = 138/182 (75.82%), Postives = 149/182 (81.87%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      M+NDG+ EFYVDFHGPSESPYHGG+WRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMVNDGMHEFYVDFHGPSESPYHGGVWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGF+NKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFINKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAAALMMRDRVAYEQ+VKEYCERYAKPEDI+AAPDKSSD EELSEDESD  DE+V G+
Sbjct: 121 NGEAAALMMRDRVAYEQRVKEYCERYAKPEDIIAAPDKSSD-EELSEDESDSCDEEVAGK 180

BLAST of IVF0016389 vs. NCBI nr
Match: XP_008438028.1 (PREDICTED: ubiquitin-conjugating enzyme E2-23 kDa isoform X1 [Cucumis melo])

HSP 1 Score: 287 bits (734), Expect = 1.09e-96
Identity = 152/183 (83.06%), Postives = 155/183 (84.70%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGFVNKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ
Sbjct: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 180

BLAST of IVF0016389 vs. NCBI nr
Match: XP_008438029.1 (PREDICTED: ubiquitin-conjugating enzyme E2-23 kDa isoform X2 [Cucumis melo])

HSP 1 Score: 282 bits (722), Expect = 3.69e-95
Identity = 149/174 (85.63%), Postives = 152/174 (87.36%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSFVSQPSDPLNGEAAALMM 120
           YKSPSIGFVNKIYHP    MSGSVCLDVINQTWSP+            DPLNGEAAALMM
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMF-----------DPLNGEAAALMM 120

Query: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 164
           RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL
Sbjct: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 163

BLAST of IVF0016389 vs. NCBI nr
Match: XP_004133859.1 (ubiquitin-conjugating enzyme E2-23 kDa isoform X1 [Cucumis sativus] >KAE8650247.1 hypothetical protein Csa_011731 [Cucumis sativus])

HSP 1 Score: 282 bits (721), Expect = 1.04e-94
Identity = 149/183 (81.42%), Postives = 154/183 (84.15%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGF+NKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFLNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESD SDEQV+GQ
Sbjct: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDSSDEQVVGQ 180

BLAST of IVF0016389 vs. NCBI nr
Match: XP_011650746.1 (ubiquitin-conjugating enzyme E2-23 kDa isoform X2 [Cucumis sativus])

HSP 1 Score: 277 bits (709), Expect = 3.53e-93
Identity = 146/174 (83.91%), Postives = 151/174 (86.78%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSFVSQPSDPLNGEAAALMM 120
           YKSPSIGF+NKIYHP    MSGSVCLDVINQTWSP+            DPLNGEAAALMM
Sbjct: 61  YKSPSIGFLNKIYHPNVDEMSGSVCLDVINQTWSPMF-----------DPLNGEAAALMM 120

Query: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQADL 164
           RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESD SDEQV+GQADL
Sbjct: 121 RDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDSSDEQVVGQADL 163

BLAST of IVF0016389 vs. NCBI nr
Match: XP_038876255.1 (ubiquitin-conjugating enzyme E2-23 kDa-like isoform X3 [Benincasa hispida])

HSP 1 Score: 272 bits (695), Expect = 9.52e-91
Identity = 142/183 (77.60%), Postives = 151/183 (82.51%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDG+QEFYVDFHGPSESPYHGG+WRIRVELPD YP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFYVDFHGPSESPYHGGVWRIRVELPDVYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSPIVPTTTSF---------VSQPSDPL 120
           YKSPSIGFVNKIYHP    MSGSVCLDVINQTWSP+      F            PSDPL
Sbjct: 61  YKSPSIGFVNKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFEVFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAAALMMRDRVAYEQ+VKEYCERYAKPED VAAPD+SSD+EELSEDESD  DEQV+GQ
Sbjct: 121 NGEAAALMMRDRVAYEQRVKEYCERYAKPEDTVAAPDESSDDEELSEDESDSGDEQVVGQ 180

BLAST of IVF0016389 vs. TAIR 10
Match: AT5G41340.1 (ubiquitin conjugating enzyme 4 )

HSP 1 Score: 208.8 bits (530), Expect = 3.4e-54
Identity = 116/177 (65.54%), Postives = 137/177 (77.40%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMD+MKL++       INDG+QEFYV+F+GP +S Y GG+W+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDMMKLMMSDYKVETINDGMQEFYVEFNGPKDSLYQGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GF+ KIYHP    +SGSVCLDVINQTWSP   +V    +F+ Q      PSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDELSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDE--SDLSDE 157
           NGEAAALMMRDR AYEQ+VKEYCE+YAKP +   + DKSSD EELSE+E  SD  D+
Sbjct: 121 NGEAAALMMRDRPAYEQRVKEYCEKYAKPGE--GSEDKSSD-EELSEEEYGSDNEDD 174

BLAST of IVF0016389 vs. TAIR 10
Match: AT1G63800.1 (ubiquitin-conjugating enzyme 5 )

HSP 1 Score: 207.6 bits (527), Expect = 7.6e-54
Identity = 114/177 (64.41%), Postives = 134/177 (75.71%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           MSSPSKRREMDLMKL++      MINDG+QEF+V+F GP +S Y GG+W+IRVELPDAYP
Sbjct: 1   MSSPSKRREMDLMKLMMSDYKVEMINDGMQEFFVEFSGPKDSIYEGGVWKIRVELPDAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GF+ KIYHP    MSGSVCLDVINQTWSP   +V    +F+ Q      PSDPL
Sbjct: 61  YKSPSVGFITKIYHPNVDEMSGSVCLDVINQTWSPMFDLVNVFETFLPQLLLYPNPSDPL 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDE--SDLSDE 157
           NGEAAALMMRDR  YEQ+VKEYCE+YAKP    A  ++ S ++E+SEDE  SD  DE
Sbjct: 121 NGEAAALMMRDRPTYEQRVKEYCEKYAKPR---ADTEEMSSDDEMSEDEYASDGDDE 174

BLAST of IVF0016389 vs. TAIR 10
Match: AT2G46030.1 (ubiquitin-conjugating enzyme 6 )

HSP 1 Score: 204.9 bits (520), Expect = 4.9e-53
Identity = 105/182 (57.69%), Postives = 133/182 (73.08%), Query Frame = 0

Query: 1   MSSPSKRREMDLMKLLI------MINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYP 60
           M+SPSKRREMD+MKL++       +ND +Q FYV FHGP++S Y GG+W+I+VELP+AYP
Sbjct: 1   MASPSKRREMDMMKLMMSDYKVDTVNDDLQMFYVTFHGPTDSLYQGGVWKIKVELPEAYP 60

Query: 61  YKSPSIGFVNKIYHP----MSGSVCLDVINQTWSP---IVPTTTSFVSQ------PSDPL 120
           YKSPS+GFVNKIYHP     SG+VCLDVINQTWSP   ++    SF+ Q      PSDP 
Sbjct: 61  YKSPSVGFVNKIYHPNVDESSGAVCLDVINQTWSPMFDLINVFESFLPQLLLYPNPSDPF 120

Query: 121 NGEAAALMMRDRVAYEQKVKEYCERYAKPEDIVAAPDKSSDEEELSEDESDLSDEQVMGQ 164
           NGEAA+L+MRDR AYE KVKEYCE+YAKPE+I++  D      E   D  D  D++++G+
Sbjct: 121 NGEAASLLMRDRAAYELKVKEYCEKYAKPEEILSDDDDDDSMSEDGSDSDDDDDDEIVGK 180

BLAST of IVF0016389 vs. TAIR 10
Match: AT1G64230.4 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0

Query: 15  LLIMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP---MS 74
           LL  + + +  +     GPS+SPY GG++ + +  P  YP+K P + F  K++HP    +
Sbjct: 64  LLCPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNSN 123

Query: 75  GSVCLDVINQTWSPIVPTTTSFVS--------QPSDPLNGEAAALMMRDRVAYEQKVKEY 129
           GS+CLD++ + WSP +  +   +S         P DPL  E A +   DR  YE   + +
Sbjct: 124 GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSW 183

BLAST of IVF0016389 vs. TAIR 10
Match: AT1G64230.5 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 86.7 bits (213), Expect = 1.9e-17
Identity = 40/125 (32.00%), Postives = 66/125 (52.80%), Query Frame = 0

Query: 15  LLIMINDGVQEFYVDFHGPSESPYHGGLWRIRVELPDAYPYKSPSIGFVNKIYHP---MS 74
           LL  + + +  +     GPS+SPY GG++ + +  P  YP+K P + F  K++HP    +
Sbjct: 64  LLCPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNVNSN 123

Query: 75  GSVCLDVINQTWSPIVPTTTSFVS--------QPSDPLNGEAAALMMRDRVAYEQKVKEY 129
           GS+CLD++ + WSP +  +   +S         P DPL  E A +   DR  YE   + +
Sbjct: 124 GSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIAHMYKTDRAKYESTARSW 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P165773.0e-5562.84Ubiquitin-conjugating enzyme E2-23 kDa OS=Triticum aestivum OX=4565 GN=UBC4 PE=1... [more]
P427484.8e-5365.54Ubiquitin-conjugating enzyme E2 4 OS=Arabidopsis thaliana OX=3702 GN=UBC4 PE=2 S... [more]
P427491.1e-5264.41Ubiquitin-conjugating enzyme E2 5 OS=Arabidopsis thaliana OX=3702 GN=UBC5 PE=2 S... [more]
P427506.9e-5257.69Ubiquitin-conjugating enzyme E2 6 OS=Arabidopsis thaliana OX=3702 GN=UBC6 PE=2 S... [more]
Q9P7R41.4e-4151.43Ubiquitin-conjugating enzyme E2 8 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
A0A1S3AW081.8e-7483.06ubiquitin-conjugating enzyme E2-23 kDa isoform X1 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A1S3AVZ63.3e-7385.63ubiquitin-conjugating enzyme E2-23 kDa isoform X2 OS=Cucumis melo OX=3656 GN=LOC... [more]
A0A5D3D1079.3e-6885.63AT-rich interactive domain-containing protein 1-like isoform X1 OS=Cucumis melo ... [more]
A0A5A7U0N09.3e-6885.63AT-rich interactive domain-containing protein 1-like isoform X1 OS=Cucumis melo ... [more]
A0A6J1D1202.1e-6775.82ubiquitin-conjugating enzyme E2 4 isoform X1 OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
XP_008438028.11.09e-9683.06PREDICTED: ubiquitin-conjugating enzyme E2-23 kDa isoform X1 [Cucumis melo][more]
XP_008438029.13.69e-9585.63PREDICTED: ubiquitin-conjugating enzyme E2-23 kDa isoform X2 [Cucumis melo][more]
XP_004133859.11.04e-9481.42ubiquitin-conjugating enzyme E2-23 kDa isoform X1 [Cucumis sativus] >KAE8650247.... [more]
XP_011650746.13.53e-9383.91ubiquitin-conjugating enzyme E2-23 kDa isoform X2 [Cucumis sativus][more]
XP_038876255.19.52e-9177.60ubiquitin-conjugating enzyme E2-23 kDa-like isoform X3 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G41340.13.4e-5465.54ubiquitin conjugating enzyme 4 [more]
AT1G63800.17.6e-5464.41ubiquitin-conjugating enzyme 5 [more]
AT2G46030.14.9e-5357.69ubiquitin-conjugating enzyme 6 [more]
AT1G64230.41.9e-1732.00ubiquitin-conjugating enzyme 28 [more]
AT1G64230.51.9e-1732.00ubiquitin-conjugating enzyme 28 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 4..129
e-value: 6.1E-25
score: 98.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 142..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..164
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 3..163
NoneNo IPR availablePANTHERPTHR24068:SF294UBIQUITIN-CONJUGATING ENZYME E2-21 KDA 1coord: 3..163
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 18..123
e-value: 6.2E-24
score: 84.2
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 1..145
score: 17.089298
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 18..124
e-value: 1.19877E-34
score: 116.533
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..140
e-value: 1.4E-31
score: 111.2
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 17..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0016389.1IVF0016389.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity