IVF0014951 (gene) Melon (IVF77) v1

Overview
NameIVF0014951
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionacyl-CoA--sterol O-acyltransferase 1-like
Locationchr04: 22697817 .. 22698913 (-)
RNA-Seq ExpressionIVF0014951
SyntenyIVF0014951
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCATAATACCTTTGAGTTAATTTCTCCATTAATGGCGGCGGCAGAGATTCGAATTTTCCTTCAAACTCTCTTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGCTTCCCCAAATTCCTCTTTCTTCTCCCCTTCTTCTTCCTCTTCCTCTACCTTCCTCTCCAATTCCAAACCCTCCATTTACAAGGCCTCTTCGGCTTCTTCATCGGTTGGCTCGCCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTCTTCCCCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAGATCCCCCAGGACACCAACCCCTCCGATCCTAATCCAATTCTTCCCCACACCTACTTTATAAAATTAGGGCTTCTAATTCTCACATTTGTCGCCGTCTATTTCAGAACTTACTTACATCCAAACCTATATCTGATCGCCTTCTGTTTCTTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAATTATTGCCGTATTTCGACGAGCCCTATCTCTCCGCTTCGTTACAGGATTTTTGGGGTAAGCGATGGAATTTAATGGCTTCTAGAATCCTCCGCCTCGCCGTTTACGATCCGTGCCGGAAACTCACTATCGGCATCGTCGGGAAGAAACCGTCGGCGATAGTGGCGATGACGGCAACATTTGCAGTCTCCGGTTTGATGCACGAACTGATTTATTTCTATATGGGAAGAGTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTAAGTCTTTCGGCGGAGATGGCGATGAAGTCAGCGGTCGGGGGTAGGTTCCGGGTGCCGAGAATTGTATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTTTTCTTCCCGCAGTTTTTTAGGTTAAAACCGGAGGTAAGAATGTTTGAAGAACACGCAGCCTTGTGGGCGTTTGTGAAGAATGTGACGGTACAGCTCATCACACCGTTTAAATTTCTCTTACAATTTCATATGAAATGA

mRNA sequence

ATCATAATACCTTTGAGTTAATTTCTCCATTAATGGCGGCGGCAGAGATTCGAATTTTCCTTCAAACTCTCTTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGCTTCCCCAAATTCCTCTTTCTTCTCCCCTTCTTCTTCCTCTTCCTCTACCTTCCTCTCCAATTCCAAACCCTCCATTTACAAGGCCTCTTCGGCTTCTTCATCGGTTGGCTCGCCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTCTTCCCCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAGATCCCCCAGGACACCAACCCCTCCGATCCTAATCCAATTCTTCCCCACACCTACTTTATAAAATTAGGGCTTCTAATTCTCACATTTGTCGCCGTCTATTTCAGAACTTACTTACATCCAAACCTATATCTGATCGCCTTCTGTTTCTTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAATTATTGCCGTATTTCGACGAGCCCTATCTCTCCGCTTCGTTACAGGATTTTTGGGGTAAGCGATGGAATTTAATGGCTTCTAGAATCCTCCGCCTCGCCGTTTACGATCCGTGCCGGAAACTCACTATCGGCATCGTCGGGAAGAAACCGTCGGCGATAGTGGCGATGACGGCAACATTTGCAGTCTCCGGTTTGATGCACGAACTGATTTATTTCTATATGGGAAGAGTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTAAGTCTTTCGGCGGAGATGGCGATGAAGTCAGCGGTCGGGGGTAGGTTCCGGGTGCCGAGAATTGTATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTTTTCTTCCCGCAGTTTTTTAGGTTAAAACCGGAGGTAAGAATGTTTGAAGAACACGCAGCCTTGTGGGCGTTTGTGAAGAATGTGACGGTACAGCTCATCACACCGTTTAAATTTCTCTTACAATTTCATATGAAATGA

Coding sequence (CDS)

ATGGCGGCGGCAGAGATTCGAATTTTCCTTCAAACTCTCTTCTTAGTTTCTTCATCTCTCTCTTTCTGTTTCTCAATTAGAAACTCAATCCCAAATGGCTTCCCCAAATTCCTCTTTCTTCTCCCCTTCTTCTTCCTCTTCCTCTACCTTCCTCTCCAATTCCAAACCCTCCATTTACAAGGCCTCTTCGGCTTCTTCATCGGTTGGCTCGCCAGTTTCAAGCTCCTCCTCTTTGCCTTCGGTAAAGGCCCTCTCTCTTCCCCTGCCGCCTCCTCCTCCCTCCGCCGCTTCCTCGCCATCGGCTCCCTCCCAATCGAGATCCCCCAGGACACCAACCCCTCCGATCCTAATCCAATTCTTCCCCACACCTACTTTATAAAATTAGGGCTTCTAATTCTCACATTTGTCGCCGTCTATTTCAGAACTTACTTACATCCAAACCTATATCTGATCGCCTTCTGTTTCTTAATTTACTTCCTCCTCGAAATTCTCATCGGTGGCGCCGCCGCCTTCGTGAAGGCGACGCTCGGAGTCGAATTATTGCCGTATTTCGACGAGCCCTATCTCTCCGCTTCGTTACAGGATTTTTGGGGTAAGCGATGGAATTTAATGGCTTCTAGAATCCTCCGCCTCGCCGTTTACGATCCGTGCCGGAAACTCACTATCGGCATCGTCGGGAAGAAACCGTCGGCGATAGTGGCGATGACGGCAACATTTGCAGTCTCCGGTTTGATGCACGAACTGATTTATTTCTATATGGGAAGAGTGGCGCCAACTTGGGAGGTGACTTGCTTCTTTTTATTACATGGAGTAAGTCTTTCGGCGGAGATGGCGATGAAGTCAGCGGTCGGGGGTAGGTTCCGGGTGCCGAGAATTGTATCGACGGGGTTTACATGGTGGTTTGTGATGACGACGAGTTCTTGGCTTTTCTTCCCGCAGTTTTTTAGGTTAAAACCGGAGGTAAGAATGTTTGAAGAACACGCAGCCTTGTGGGCGTTTGTGAAGAATGTGACGGTACAGCTCATCACACCGTTTAAATTTCTCTTACAATTTCATATGAAATGA

Protein sequence

MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Homology
BLAST of IVF0014951 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.3e-63
Identity = 143/338 (42.31%), Postives = 200/338 (59.17%), Query Frame = 0

Query: 9   FLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIG 68
           F++   LV  SL + F I   +P G  + +   P F +F  +P    +LHL G+  FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 69  WLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEI-----PQDTNPSDPNPILPHT 128
           WLA+FKLLLFA G+GPLSS     SL  FLA+  LPI+I     P  T+  + +   P  
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 129 YFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPY 188
           Y IK   ++L   A  + T L   + L  +   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 189 FDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSG 248
           F++PYL+ SLQDFWG+RWNLM + ILR  VY+P  +L   ++G   S I+A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 249 LMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFVM 308
           +MHELI+FYMGR+ P W++  FFL++G   + E+A+K  + GR+R P+ +S   T  FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 309 TTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQL 342
            T+ WLF P+F R     +  +E+AA+ AF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of IVF0014951 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 5.5e-54
Identity = 136/347 (39.19%), Postives = 196/347 (56.48%), Query Frame = 0

Query: 5   EIRIFLQTLFLVSSSLSFCFSIRNSIPNGFP-KFLFLLPFFFLFLYLPLQFQTLHLQGLF 64
           E++ F +      ++  +C  +    P+G   + L LLP   LF++LPL+  + HL G  
Sbjct: 6   ELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLGGPT 65

Query: 65  GFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQD-TNPSDPNPILPH 124
             ++ WLA+FKLLLFAF  GPLS+P  S SL  F++   LPI+   D +N  + N     
Sbjct: 66  ALYLVWLANFKLLLFAFHLGPLSNP--SLSLLHFISTTLLPIKFRDDPSNDHEKNKRTLS 125

Query: 125 TYFIKLGLLI--LTFVA----VY-FRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKAT 184
             + K+ L +  L F A    +Y FR  L   +  + +CF  Y   EI +  +A   +AT
Sbjct: 126 FEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAVIARAT 185

Query: 185 LGVELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAM 244
           LG++L P F+EPYL+ SLQDFWG+RWNLM S IL L  Y P R++    V  +     AM
Sbjct: 186 LGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWEVAGAM 245

Query: 245 TATFAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVST 304
              F VSGLMHE+ +FY+ R  P+WEVT FF+LHGV  + EM +K AV G+ R+ R VS 
Sbjct: 246 LVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLRREVSG 305

Query: 305 GFTWWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLI 343
             T  FVM T  WLF PQ  R   +++  +E+  ++ + +   + L+
Sbjct: 306 ALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLL 350

BLAST of IVF0014951 vs. ExPASy Swiss-Prot
Match: Q9FJ73 (Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=AT2 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.3e-50
Identity = 131/346 (37.86%), Postives = 188/346 (54.34%), Query Frame = 0

Query: 5   EIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFG 64
           E+R  ++       S+S+C+ I + I  G  + L LLP F +FL LPL F ++H   + G
Sbjct: 4   ELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCVISG 63

Query: 65  FFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTY 124
           FF  WLA+FKL LFAF + PLS     S+L RF      PI+I ++ + +  +      +
Sbjct: 64  FFFTWLANFKLFLFAFDQEPLS--PLPSNLTRFFCFACFPIKINKNPSSNRIHNKPMSKW 123

Query: 125 FIKLGLLILTFVAVYFRTYLHPNL----YLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 184
            +   LLI +F+   +R      L    +L  F   +Y   E+++    A +   LG E+
Sbjct: 124 VLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLLGCEM 183

Query: 185 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 244
            P F++PYL+ SLQ+FW +RWNLM   +LR AV+ P ++    ++G   +    M ATF 
Sbjct: 184 EPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGMLATFI 243

Query: 245 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFR---VPRIVSTGF 304
           VSGLMHELIYFY+ R +PTWEVTCFFLLHGV    E+AMK     R R    PR   +G 
Sbjct: 244 VSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRRAVSGL 303

Query: 305 T-WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLI 343
               F++ T+ WLF+PQ  R     R+  E   +   VK   V ++
Sbjct: 304 AITVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKRHVVCIL 342

BLAST of IVF0014951 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 6.3e-50
Identity = 124/319 (38.87%), Postives = 182/319 (57.05%), Query Frame = 0

Query: 19  SLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIGWLASFKLLLF 78
           S+S+C+     I +G  + L +LP   LFL LPL   ++H  G   FF+ WLA+FKL+LF
Sbjct: 18  SISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTAFFLSWLANFKLILF 77

Query: 79  AFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTYFIKLGLLILTFVAV 138
           +F +GPL      S+L RF+     PI++ Q  NP   N +    + +KL    +     
Sbjct: 78  SFDQGPLF--PVPSNLSRFVCFTCFPIKLQQ--NPKPQNQMPKWGFAVKLAFFGVLLHMY 137

Query: 139 YFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSASLQDFWG 198
            ++ ++ P + L+ +   IY   EIL+      +  +L  +L P+F+EPYLS SLQDFWG
Sbjct: 138 EYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWG 197

Query: 199 KRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSGLMHELIYFYMGRVAP 258
           +RWNLM   ILR AVY P R++    +    +  + + A+F VSG++HELI+FY  R +P
Sbjct: 198 RRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVHELIFFYFTRESP 257

Query: 259 TWEVTCFFLLHGVSLSAEMAMK-SAVGGRFRVPRIVSTGFTWWFVMTTSSWLFFPQFFRL 318
           T EVT FF+LHGV  +AE A K + +  R++V ++VS   T  FV+ T  WLFFP   R 
Sbjct: 258 TGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVMTGGWLFFPHLARS 317

Query: 319 KPEVRMFEEHAALWAFVKN 337
               R+ +E      FVK+
Sbjct: 318 GMIERLADEAFLFIGFVKH 332

BLAST of IVF0014951 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 4.1e-49
Identity = 129/311 (41.48%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 5   EIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFG 64
           E++ F++       S+S+C+ +   I  G  + L +LP   LFL  PL F  +H  G   
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 65  FFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTY 124
           FF+ WLA+FKL+LF+F +GPLS      +L RF+ I   PI+ PQ  NP+  N  +P   
Sbjct: 64  FFLSWLANFKLILFSFDQGPLS--PLPRTLSRFICITCFPIK-PQQ-NPNIQNYKIPIWL 123

Query: 125 F-IKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPY 184
           F IK+ + ++      ++ YL P L L+     I+  LEI+     A V  TLG +L P 
Sbjct: 124 FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQ 183

Query: 185 FDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSG 244
            +EPYL+ SLQDFWG+RWNLM   ILR AVY P R++    V    +  + + A F VSG
Sbjct: 184 SNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSG 243

Query: 245 LMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGR--FRVPRIVSTGFTWWF 304
            +HE+++FY+ R  PT EVT FFLLHGV   AE+A+K +   R  +RV   VS   T  F
Sbjct: 244 AVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLTVGF 303

Query: 305 VMTTSSWLFFP 313
           V+ TS W FFP
Sbjct: 304 VVVTSGWFFFP 310

BLAST of IVF0014951 vs. ExPASy TrEMBL
Match: A0A5D3BDP7 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold939G00060 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.0e-196
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60
           MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ
Sbjct: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60

Query: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120
           GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL
Sbjct: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120

Query: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180
           PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL
Sbjct: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180

Query: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240
           LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA
Sbjct: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240

Query: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300
           VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW
Sbjct: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300

Query: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 355
           FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Sbjct: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354

BLAST of IVF0014951 vs. ExPASy TrEMBL
Match: A0A1S3CFI3 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC103500342 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.0e-196
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60
           MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ
Sbjct: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60

Query: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120
           GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL
Sbjct: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120

Query: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180
           PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL
Sbjct: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180

Query: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240
           LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA
Sbjct: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240

Query: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300
           VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW
Sbjct: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300

Query: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 355
           FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Sbjct: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354

BLAST of IVF0014951 vs. ExPASy TrEMBL
Match: A0A0A0LES4 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-168
Identity = 307/345 (88.99%), Postives = 322/345 (93.33%), Query Frame = 0

Query: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62
           AAEIRIFLQTL LVSSSLSFCFSIRNSIPNGF +FL +LPFF LFLY+PLQFQT+HLQG 
Sbjct: 2   AAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGS 61

Query: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122
            GFFIGWLASFKLLLFAFGKGPL S AASSSLRRFLAIGSLPIEIPQDTN S PNPILP 
Sbjct: 62  IGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILPP 121

Query: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182
           TYFIKL LLILT VA+YF+ YLHP LYLI FCF+IYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 122 TYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLP 181

Query: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242
           YFDEPYLS SLQDFWGKRWNL+ SRILRLAVYDPCRKLT+GIVGKK SA+VA+TATFAVS
Sbjct: 182 YFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAVS 241

Query: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302
           GLMHELIYFYMGR+APTWEVTCFFLLHGVSLSAEMAMKSAV GR +VPRI+STGFTWWFV
Sbjct: 242 GLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFV 301

Query: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 348
           MTTSSWLFFPQFFRLKP+VRM EEHAAL AFVKNVTVQLITPFKF
Sbjct: 302 MTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKF 346

BLAST of IVF0014951 vs. ExPASy TrEMBL
Match: A0A5A7U2E1 (Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G001430 PE=3 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 0

Query: 59  LQGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 118
           L GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP
Sbjct: 348 LFGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 407

Query: 119 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 178
           ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV
Sbjct: 408 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 467

Query: 179 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 238
           ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT
Sbjct: 468 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 527

Query: 239 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 298
           FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT
Sbjct: 528 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 587

Query: 299 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 355
           WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Sbjct: 588 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 643

BLAST of IVF0014951 vs. ExPASy TrEMBL
Match: A0A6J1IX47 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481451 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.5e-131
Identity = 249/355 (70.14%), Postives = 289/355 (81.41%), Query Frame = 0

Query: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62
           AAEI  F+QTLFL+SSSL  CFSIRNSIPNGF KF+F+LPFFFLFLY+PLQFQT+HL+G 
Sbjct: 2   AAEIHAFIQTLFLISSSLCLCFSIRNSIPNGFLKFIFVLPFFFLFLYIPLQFQTIHLRGT 61

Query: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNP---SDPNPI 122
            GFFIGWL SFKLLLFAFGKGPL S A SSSLRRFLA+ SLPI+IP+   P   SDP+P+
Sbjct: 62  LGFFIGWLGSFKLLLFAFGKGPLCSAATSSSLRRFLAVASLPIDIPEHKPPPNRSDPDPL 121

Query: 123 LPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVE 182
           LP ++  KLG LILTF A+YF+   +PN+ LI FC LIY LLEILIGGA A  +A LGVE
Sbjct: 122 LPLSFAAKLGFLILTFAAIYFKNRFYPNVVLIFFCLLIYLLLEILIGGATAVAEAVLGVE 181

Query: 183 LLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATF 242
           LLP+FDEPYLS SLQDFWG+RWNL++SRILR AV+DPCRKL   +VGKKP+ I+A+ ATF
Sbjct: 182 LLPFFDEPYLSDSLQDFWGRRWNLVSSRILRPAVFDPCRKLAGTVVGKKPAVIIAVMATF 241

Query: 243 AVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTW 302
           AVSG+MHELIYFYMGR+APTWEV+ FFL+HGV L+AEMA+KSAVG RFR PR+VS   T 
Sbjct: 242 AVSGVMHELIYFYMGRLAPTWEVSWFFLIHGVCLAAEMAVKSAVGRRFRAPRVVSGVLTM 301

Query: 303 WFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 355
            FVM TS WLFFPQ  R K +VRM EE+AAL AFVKNVT +LI P    + F +K
Sbjct: 302 GFVMATSFWLFFPQLLRFKADVRMLEEYAALGAFVKNVTAELIGP--LFISFQVK 354

BLAST of IVF0014951 vs. NCBI nr
Match: XP_008461835.1 (PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >TYJ97433.1 acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 692 bits (1787), Expect = 3.77e-251
Identity = 354/354 (100.00%), Postives = 354/354 (100.00%), Query Frame = 0

Query: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60
           MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ
Sbjct: 1   MAAAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQ 60

Query: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120
           GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL
Sbjct: 61  GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPIL 120

Query: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180
           PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL
Sbjct: 121 PHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 180

Query: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240
           LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA
Sbjct: 181 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 240

Query: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300
           VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW
Sbjct: 241 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWW 300

Query: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354
           FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Sbjct: 301 FVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354

BLAST of IVF0014951 vs. NCBI nr
Match: XP_004149741.2 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58561.1 hypothetical protein Csa_001738 [Cucumis sativus])

HSP 1 Score: 600 bits (1548), Expect = 7.92e-215
Identity = 307/345 (88.99%), Postives = 322/345 (93.33%), Query Frame = 0

Query: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62
           AAEIRIFLQTL LVSSSLSFCFSIRNSIPNGF +FL +LPFF LFLY+PLQFQT+HLQG 
Sbjct: 2   AAEIRIFLQTLLLVSSSLSFCFSIRNSIPNGFLRFLLILPFFSLFLYIPLQFQTIHLQGS 61

Query: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPH 122
            GFFIGWLASFKLLLFAFGKGPL S AASSSLRRFLAIGSLPIEIPQDTN S PNPILP 
Sbjct: 62  IGFFIGWLASFKLLLFAFGKGPLCSAAASSSLRRFLAIGSLPIEIPQDTNRSHPNPILPP 121

Query: 123 TYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLP 182
           TYFIKL LLILT VA+YF+ YLHP LYLI FCF+IYFLLEILIGGAAAFVKATLGVELLP
Sbjct: 122 TYFIKLLLLILTLVAIYFKNYLHPTLYLIVFCFVIYFLLEILIGGAAAFVKATLGVELLP 181

Query: 183 YFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVS 242
           YFDEPYLS SLQDFWGKRWNL+ SRILRLAVYDPCRKLT+GIVGKK SA+VA+TATFAVS
Sbjct: 182 YFDEPYLSVSLQDFWGKRWNLLTSRILRLAVYDPCRKLTVGIVGKKASAMVALTATFAVS 241

Query: 243 GLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFV 302
           GLMHELIYFYMGR+APTWEVTCFFLLHGVSLSAEMAMKSAV GR +VPRI+STGFTWWFV
Sbjct: 242 GLMHELIYFYMGRLAPTWEVTCFFLLHGVSLSAEMAMKSAVRGRLKVPRIISTGFTWWFV 301

Query: 303 MTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKF 347
           MTTSSWLFFPQFFRLKP+VRM EEHAAL AFVKNVTVQLITPFKF
Sbjct: 302 MTTSSWLFFPQFFRLKPDVRMMEEHAALGAFVKNVTVQLITPFKF 346

BLAST of IVF0014951 vs. NCBI nr
Match: KAA0048417.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 584 bits (1506), Expect = 6.63e-204
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 0

Query: 59  LQGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 118
           L GLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP
Sbjct: 348 LFGLFGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNP 407

Query: 119 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 178
           ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV
Sbjct: 408 ILPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGV 467

Query: 179 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 238
           ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT
Sbjct: 468 ELLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTAT 527

Query: 239 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 298
           FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT
Sbjct: 528 FAVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFT 587

Query: 299 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354
           WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK
Sbjct: 588 WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 643

BLAST of IVF0014951 vs. NCBI nr
Match: XP_038905685.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 523 bits (1348), Expect = 2.80e-184
Identity = 273/355 (76.90%), Postives = 302/355 (85.07%), Query Frame = 0

Query: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62
           AAEIRIFLQTLFLVSSSL FCF+IRNSIPNGF KFL +LPFFFLFLY+PLQFQ++H QG 
Sbjct: 2   AAEIRIFLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQSIHFQGS 61

Query: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQD---TNPSDPNPI 122
            GFFIGWL SFKLLLFAFGKGPL S AASSSLR FLA+GSLPIEIP      N S  NP+
Sbjct: 62  IGFFIGWLGSFKLLLFAFGKGPLCSAAASSSLRHFLAVGSLPIEIPDHKPAANHSHSNPV 121

Query: 123 LPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVE 182
           LP ++  KLGLLILT VA+YF+   HPN  LI FCF+IY LLEIL+GGAAA  KATLGVE
Sbjct: 122 LPLSFSSKLGLLILTIVAIYFKNLFHPNAILIFFCFVIYLLLEILLGGAAALAKATLGVE 181

Query: 183 LLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATF 242
           LLPYFDEPYLS SLQDFWG+RWNLM SRILRL VYDPCRK+TIGIVG+KP+A++AMTATF
Sbjct: 182 LLPYFDEPYLSDSLQDFWGRRWNLMTSRILRLVVYDPCRKITIGIVGRKPAAMMAMTATF 241

Query: 243 AVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTW 302
           AVSGLMHELIYFYMGR+APTWEVTCFFLLHGV L+AEMA+KSAVGGRFR+P +VS   T 
Sbjct: 242 AVSGLMHELIYFYMGRLAPTWEVTCFFLLHGVCLAAEMAIKSAVGGRFRLPGVVSAALTI 301

Query: 303 WFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFLLQFHMK 354
            FVMTTS WLFFPQF +LK +VRMFEE+AAL AFVK+VT QLITP    + FH K
Sbjct: 302 GFVMTTSFWLFFPQFLKLKSDVRMFEEYAALGAFVKSVTGQLITPLN--ISFHFK 354

BLAST of IVF0014951 vs. NCBI nr
Match: XP_038903196.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 481 bits (1237), Expect = 2.23e-167
Identity = 248/349 (71.06%), Postives = 282/349 (80.80%), Query Frame = 0

Query: 3   AAEIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGL 62
           AAEI   LQTLFLVSSSL FCF+IRNSIPNGF KFL +LPFFFLFLY+PLQFQT+H QG 
Sbjct: 2   AAEIHSLLQTLFLVSSSLCFCFTIRNSIPNGFLKFLLILPFFFLFLYIPLQFQTIHFQGP 61

Query: 63  FGFFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNP---SDPNPI 122
            GFFIGWL SFKLLLFAFGKGPLSS A SSSL RFLA+GSLPIEIP   +P   S+P  +
Sbjct: 62  IGFFIGWLGSFKLLLFAFGKGPLSSAATSSSLPRFLAVGSLPIEIPDHQSPPNHSNPKLV 121

Query: 123 LPHTYFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVE 182
           LP +Y  KL  LILT VA+ F+ + HPN  L+ +C LIYF LE LIG  AA  KA LGVE
Sbjct: 122 LPFSYSAKLAFLILTMVAINFKNHFHPNAALLFYCLLIYFFLEFLIGSTAAVAKAVLGVE 181

Query: 183 LLPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATF 242
           LLPYF+EPYLS SLQDFWG+RWNLM SRILRL VYDPCRKLTIG++G+KP+ ++A+ ATF
Sbjct: 182 LLPYFNEPYLSDSLQDFWGRRWNLMTSRILRLTVYDPCRKLTIGVIGRKPATMLAVMATF 241

Query: 243 AVSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTW 302
            VSGLMHELIYFY+GR+APTWEVTCFFL+HGV L  EMA+K A GGRFR P++VS   T 
Sbjct: 242 VVSGLMHELIYFYIGRLAPTWEVTCFFLVHGVCLVTEMAVKLAAGGRFRPPQVVSIPLTI 301

Query: 303 WFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLITPFKFL 348
            FVM T  WLFFPQ  R K +VRMFEE+AA+ AFVK++TV LITPFKFL
Sbjct: 302 GFVMGTGCWLFFPQCLRAKMDVRMFEEYAAIGAFVKSITVDLITPFKFL 350

BLAST of IVF0014951 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 245.0 bits (624), Expect = 9.2e-65
Identity = 143/338 (42.31%), Postives = 200/338 (59.17%), Query Frame = 0

Query: 9   FLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIG 68
           F++   LV  SL + F I   +P G  + +   P F +F  +P    +LHL G+  FFI 
Sbjct: 4   FIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIA 63

Query: 69  WLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEI-----PQDTNPSDPNPILPHT 128
           WLA+FKLLLFA G+GPLSS     SL  FLA+  LPI+I     P  T+  + +   P  
Sbjct: 64  WLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPLI 123

Query: 129 YFIKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPY 188
           Y IK   ++L   A  + T L   + L  +   IYF LEI++   AA V+A   +EL P 
Sbjct: 124 YTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEPQ 183

Query: 189 FDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSG 248
           F++PYL+ SLQDFWG+RWNLM + ILR  VY+P  +L   ++G   S I+A   TF VSG
Sbjct: 184 FNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQL-FSVLGPNYSQILAAFGTFVVSG 243

Query: 249 LMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFRVPRIVSTGFTWWFVM 308
           +MHELI+FYMGR+ P W++  FFL++G   + E+A+K  + GR+R P+ +S   T  FVM
Sbjct: 244 IMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLTFVM 303

Query: 309 TTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQL 342
            T+ WLF P+F R     +  +E+AA+ AF   V  +L
Sbjct: 304 VTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEVRRKL 340

BLAST of IVF0014951 vs. TAIR 10
Match: AT5G55370.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-51
Identity = 131/346 (37.86%), Postives = 188/346 (54.34%), Query Frame = 0

Query: 5   EIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFG 64
           E+R  ++       S+S+C+ I + I  G  + L LLP F +FL LPL F ++H   + G
Sbjct: 4   ELRNLIKVWISALISISYCYYISSKISKGVLRLLSLLPIFIIFLLLPLFFSSVHFCVISG 63

Query: 65  FFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTY 124
           FF  WLA+FKL LFAF + PLS     S+L RF      PI+I ++ + +  +      +
Sbjct: 64  FFFTWLANFKLFLFAFDQEPLS--PLPSNLTRFFCFACFPIKINKNPSSNRIHNKPMSKW 123

Query: 125 FIKLGLLILTFVAVYFRTYLHPNL----YLIAFCFLIYFLLEILIGGAAAFVKATLGVEL 184
            +   LLI +F+   +R      L    +L  F   +Y   E+++    A +   LG E+
Sbjct: 124 VLAFKLLIFSFLLHVYRNNYDSGLSRFAFLALFTIHVYLEAELILVFVGALMSMLLGCEM 183

Query: 185 LPYFDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFA 244
            P F++PYL+ SLQ+FW +RWNLM   +LR AV+ P ++    ++G   +    M ATF 
Sbjct: 184 EPVFNDPYLATSLQEFWSRRWNLMVPAVLRPAVHIPVQRFCAPLLGLHRAFYAGMLATFI 243

Query: 245 VSGLMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGRFR---VPRIVSTGF 304
           VSGLMHELIYFY+ R +PTWEVTCFFLLHGV    E+AMK     R R    PR   +G 
Sbjct: 244 VSGLMHELIYFYVIRKSPTWEVTCFFLLHGVVTCLEIAMK-----RMRWLPTPRRAVSGL 303

Query: 305 T-WWFVMTTSSWLFFPQFFRLKPEVRMFEEHAALWAFVKNVTVQLI 343
               F++ T+ WLF+PQ  R     R+  E   +   VK   V ++
Sbjct: 304 AITVFLLVTAGWLFYPQMLRNDVHKRVISECLLVIDVVKRHVVCIL 342

BLAST of IVF0014951 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 199.5 bits (506), Expect = 4.5e-51
Identity = 124/319 (38.87%), Postives = 182/319 (57.05%), Query Frame = 0

Query: 19  SLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIGWLASFKLLLF 78
           S+S+C+     I +G  + L +LP   LFL LPL   ++H  G   FF+ WLA+FKL+LF
Sbjct: 18  SISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSGSTAFFLSWLANFKLILF 77

Query: 79  AFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTYFIKLGLLILTFVAV 138
           +F +GPL      S+L RF+     PI++ Q  NP   N +    + +KL    +     
Sbjct: 78  SFDQGPLF--PVPSNLSRFVCFTCFPIKLQQ--NPKPQNQMPKWGFAVKLAFFGVLLHMY 137

Query: 139 YFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSASLQDFWG 198
            ++ ++ P + L+ +   IY   EIL+      +  +L  +L P+F+EPYLS SLQDFWG
Sbjct: 138 EYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCDLEPHFNEPYLSTSLQDFWG 197

Query: 199 KRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSGLMHELIYFYMGRVAP 258
           +RWNLM   ILR AVY P R++    +    +  + + A+F VSG++HELI+FY  R +P
Sbjct: 198 RRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASFLVSGVVHELIFFYFTRESP 257

Query: 259 TWEVTCFFLLHGVSLSAEMAMK-SAVGGRFRVPRIVSTGFTWWFVMTTSSWLFFPQFFRL 318
           T EVT FF+LHGV  +AE A K + +  R++V ++VS   T  FV+ T  WLFFP   R 
Sbjct: 258 TGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQMVSRLLTVGFVVMTGGWLFFPHLARS 317

Query: 319 KPEVRMFEEHAALWAFVKN 337
               R+ +E      FVK+
Sbjct: 318 GMIERLADEAFLFIGFVKH 332

BLAST of IVF0014951 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.9e-50
Identity = 129/311 (41.48%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 5   EIRIFLQTLFLVSSSLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFG 64
           E++ F++       S+S+C+ +   I  G  + L +LP   LFL  PL F  +H  G   
Sbjct: 4   ELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSGCMA 63

Query: 65  FFIGWLASFKLLLFAFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTY 124
           FF+ WLA+FKL+LF+F +GPLS      +L RF+ I   PI+ PQ  NP+  N  +P   
Sbjct: 64  FFLSWLANFKLILFSFDQGPLS--PLPRTLSRFICITCFPIK-PQQ-NPNIQNYKIPIWL 123

Query: 125 F-IKLGLLILTFVAVYFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPY 184
           F IK+ + ++      ++ YL P L L+     I+  LEI+     A V  TLG +L P 
Sbjct: 124 FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLGCDLEPQ 183

Query: 185 FDEPYLSASLQDFWGKRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSG 244
            +EPYL+ SLQDFWG+RWNLM   ILR AVY P R++    V    +  + + A F VSG
Sbjct: 184 SNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFAAFLVSG 243

Query: 245 LMHELIYFYMGRVAPTWEVTCFFLLHGVSLSAEMAMKSAVGGR--FRVPRIVSTGFTWWF 304
            +HE+++FY+ R  PT EVT FFLLHGV   AE+A+K +   R  +RV   VS   T  F
Sbjct: 244 AVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWRVSPTVSRLLTVGF 303

Query: 305 VMTTSSWLFFP 313
           V+ TS W FFP
Sbjct: 304 VVVTSGWFFFP 310

BLAST of IVF0014951 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 196.4 bits (498), Expect = 3.8e-50
Identity = 127/319 (39.81%), Postives = 178/319 (55.80%), Query Frame = 0

Query: 19  SLSFCFSIRNSIPNGFPKFLFLLPFFFLFLYLPLQFQTLHLQGLFGFFIGWLASFKLLLF 78
           S+S+C+ I   I +G P+FL + P   LFL LPL F +LHL  +  FF+ WLA+FKL+LF
Sbjct: 18  SISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSLITAFFLTWLANFKLILF 77

Query: 79  AFGKGPLSSPAASSSLRRFLAIGSLPIEIPQDTNPSDPNPILPHTYFIKLGLLILTFVAV 138
           +F KGPL      ++  RF      PI++ Q  NP   N +    + IKL +  +     
Sbjct: 78  SFDKGPLI--PIPTNFPRFFCFTCFPIKVQQ--NPKSQNHLPKLVFAIKLAIFAVLLHLY 137

Query: 139 YFRTYLHPNLYLIAFCFLIYFLLEILIGGAAAFVKATLGVELLPYFDEPYLSASLQDFWG 198
            +R  L P + L  +   +Y  +EI++      V  +LG +L P  ++PYL+ SLQDFWG
Sbjct: 138 SYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGCDLEPQSNKPYLATSLQDFWG 197

Query: 199 KRWNLMASRILRLAVYDPCRKLTIGIVGKKPSAIVAMTATFAVSGLMHELIYFYMGRVAP 258
           +RWNLM   ILR AVY P R+++   +    +    + A F VSGL+HEL++FY+ R  P
Sbjct: 198 RRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAAFIVSGLVHELLFFYLIREMP 257

Query: 259 TWEVTCFFLLHGVSLSAEMAM--KSAVGGRFRVPRIVSTGFTWWFVMTTSSWLFFPQFFR 318
           T EVT FF+LHGV  + E+A+  K+ V  R+R+   VS   T  FV  T  WLF PQ  R
Sbjct: 258 TGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLSPGVSRVLTVGFVFVTGGWLFTPQLKR 317

Query: 319 LKPEVRMFEEHAALWAFVK 336
                R   E   L  F+K
Sbjct: 318 SGVMERFTSEAVLLVEFIK 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SV071.3e-6342.31Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Q9XGY65.5e-5439.19Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ... [more]
Q9FJ736.3e-5037.86Probable long-chain-alcohol O-fatty-acyltransferase 2 OS=Arabidopsis thaliana OX... [more]
Q9FJ756.3e-5038.87Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX... [more]
Q9FJ744.1e-4941.48Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A5D3BDP73.0e-196100.00Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3CFI33.0e-196100.00acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo OX=3656 GN=LOC10350034... [more]
A0A0A0LES42.0e-16888.99MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684670 PE=... [more]
A0A5A7U2E11.5e-16399.66Acyl-CoA--sterol O-acyltransferase 1-like OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A6J1IX471.5e-13170.14acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_008461835.13.77e-251100.00PREDICTED: acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo] >TYJ97433.1 ... [more]
XP_004149741.27.92e-21588.99acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus] >KGN58561.1 hypothetical ... [more]
KAA0048417.16.63e-20499.66acyl-CoA--sterol O-acyltransferase 1-like [Cucumis melo var. makuwa][more]
XP_038905685.12.80e-18476.90acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida][more]
XP_038903196.12.23e-16771.06acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT3G51970.19.2e-6542.31acyl-CoA sterol acyl transferase 1 [more]
AT5G55370.14.5e-5137.86MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55350.14.5e-5138.87MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55360.12.9e-5041.48MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55340.13.8e-5039.81MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 182..267
e-value: 4.3E-16
score: 58.9
NoneNo IPR availablePANTHERPTHR31595:SF46ACYL-COA--STEROL O-ACYLTRANSFERASE 1coord: 4..339
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 4..339

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0014951.2IVF0014951.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity