IVF0014283 (gene) Melon (IVF77) v1

Overview
NameIVF0014283
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionSeparase
Locationchr03: 21728074 .. 21731469 (+)
RNA-Seq ExpressionIVF0014283
SyntenyIVF0014283
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTATGTTAGTCAGAAGCCTGCATATGGCATTAAGAATCTCCATAAAAACAGATCAGCTGCTCGTGATGTGTGGTCATTTGATAAAATATCATGGGACGTAGAAGGTTGCTATCTTAGTCCTTTTAATATACTTCAGTGTTATCTTGAAAGCACTCTTCAGGTAAAACTTTTCTTTTATGTTAAAACAGATGTGCGATCCCTCTGCTTGCTAACATATTTCATATGGGTATGCTATTGCTTATATAACATCCAGGTTGGTCTTGTTCATGAGATCATTGGAAATGGCTCTGAGGCTGAAACACTTTTAATGTGGGGAAAAAGTATCTCCTGCTTGCAAAGCTTGCCACTGTTTGAAGTTGCTTTCTCTTCTGCTTTAGGTACTTATATAGTACGAATTGTTTACTTGGTCTCATGTTCCAGTGGATATTGATTGTTGAATGTATACTTGCTCGAAAGAACTGTATAATAGGTAATTGTTGTGTACTTCTGGCAGGAAAATTATATAGAAAGAAAGAGTTGTGGAGTTCATCACAAAAGGAACTTGAAGGAGCCAAACAGATTTTAAAGGATAGCATTACTTCTTGTTTGAAGTGCAGATTGGTGCTTGAAGTAAAAGTTCATCAACATCTTGGAGATCTGTCCCGCGATATGTATGTAAATGCTAAAGGGATAATATCTGAAGAGCGATTAATAAATGCAGAAGGGTTTTATAATTTAGCTCTTGAGAAACTAAATCTTTCTACATGGAAAAATTCTATTAGTGGTATAGATGAAGAAACATTTCTTTCATCTTTGACAATTCAAGTGGAAAGGCCAAAGGACAAAAGGGATGGTAAAAAGGCTAAAAAAATAACAAATGCTCCCAAGTCTTTCCAAATGGACCAGTGCGTAAATCCTCGGAGTAATATGAGGTTGACTCGCTCTAGATGTCGGAAGATTCAGGGCCAAAGTGCAAGCAATTCCAATGATGTAGAAGTTGACCTTTCTGTGCATCTGAAAACCACATTCCTGATATTTCTAGTGCGTTGGGCCAAAAGCAATCACACTTGCAAGTGAATAGTTGCACACAAGGGAGAGAAGCATCATGCAAAAATGGGAAAGTTGGATGTTGGCAATGTCTACCAATGGAACTAATTGAAGCCGGACAAATGAACAATTTCATATATCTCAAGTGGGAGTTTGTTCGTAGACGGCTTGTGTTAAAACAGCTTTCTGGCATAGGTATCAAGTTTAATACCATAAAATTCTTTAGTTTAGTGATTAGCATGCGCACAAATACTTTTGTTGGTATAACCATCCAATTAAATAAATGCTTATTGTCAAGCTTTTATGATGAAGAAGTTCTTTACTTTTTTCTGTGTCAATTTTGAACAAAAATGTCAATGGCCATTGTAGTTAACTTTTAGTTAAGACTTGAGTATGACCACCATTTTGCTACAATTTGTATTAAATATTCTTCATTGCCCATTGGACGTAGGGAATGATTTTAGTATTAATTATATTATCATTATTTTCTCTGTTGTTTGTTTCTAGGTAAGTGCTCAGAGATGCGTGGTCAAATTCATCAAACACATGAAACTATTTTGAAAAGCATGTCGTTCATAGTAAGCAGAAATCTCTTCTTTCAAGAGCACTATGCGATTGAACCCACAGTTTTGCTTGAGTTAATTGGAAAGGAGGTCCATGGAGATGTGTTTGCTGTTGAGAGGGCATCGGTTCTCTATGAAATTTGCTGGTTCTCTTTGAAGAATACAATATGCAAATACCAAGTATGTATAGTTAATCTATGGTCTATATATTCCACATAAATGGCTATGTCTATTGTTTCCTCTCTTATCTTACCTTTCGTATAAATTAGATATTTCCATCCATATTGTGCACCTATTCACCACCGTGTTCTTGAAATTTAGCATTTTAAGCAAAAAGTCATTTCTCTTCATATTGATTACAATGAAAATTTGTTTCCCTTGCCCCTGAATATTGGATACCATTGAGGGTTTGCAGAACAAAGCTATCTTGAATCTTGCATATATAGATACATACGATGTTTCCCCACTTGGGACAAGAAAATTGGGTTCTTGCTTTGGAGTGAGGATTAAACGTATTCTTAGAACAACGCTTTGGAGCATTGCTTGCATTTTCATTTTGTTTTGGTAGCTGTTTTGTTTTAGGTTCAGTCTTTGTACTGTTTTGCTTCTATTCTCTTCCTTTATTTCTGTCTCAGCCCTCTGTATTAAGTTGGACTTTGTTTCGTTGTATTTGGATTAAATTTTTTCTTGCTTTATTTTGGGATATGTTGTTTCGGTGATAAGGGGGTGTCAACCTAGTTGAGATGTCTGGGTACACCTTTTGATCCCCTTTGTTTATTGCTCTCTAGGCTTTTTTATTCCACTCGCTGTATAATTCTCTAGTACTTTGAGTTTTATTATTAATAAACATATTCTTAGATAACACATTAAGTTAAGTTTGCTAAAACATAGAAATATTGTCTTTCCTTAAATCAGCTGTTATACTATAACTCCCTTTTCTGGTTACTTCTTGGCAGGATTATTTGTTGTCCTTTGTCTGAGGTTCACTCTGAAACATTGGTTTATTGGCTGAGGGTTGCCCTTGTACTTTGCTGTGAGGTTCCCGTACTTTTTCAAAAGGTCTAATTTAGTCATATCAGTTATAAATGTAAAATGTTTTATTTTATTATTTTTTTAAAATTTTGGAAATTAGTTAATATGTTTTGTCCCTTTTCATCGTTAATGTGTTCCTTTTTTCTTTTTATCAGTAATCATACTAATTGATTTTTTGTTCGTAAGCACGTTACTTAATTTAGCAATTTGCTTTGGTGATTGTACATTCTTCCATAATACATTTATGTTTGATTTTTCATATCATTAGTCACGCATCCTGAAGTTTTCCAAATATATCACATAACTTTATGCGATGCTTATTTAGGGATTCAATGCTTGTTATCTTAAATGAATTTGAACTCCATTAGGATGCTGAGTTAAATGATTGTGGTTTTTCTCGATAGATGCAAAGCTACAGTTAGGAACTATGCGTATACAACAAAAATTGTTTTTCCATTAACTTTTCTTCATTTTGTTTGTGCTTCTGTAAAATCTATTCCTCCTGTTCTATGTATTTATGTTATTTCTTGGTGGTTAACTTTGATTCTTTTGTTACTGAACAGGTTTCAAGATTGCTTGCTGTTATGCATGTAATTTCTTCAACAAGTGAGCTATTTTCTTTGGCATCTTCCAACACAATACTAGCAGACAGTCACTGGGCTTCATATTTTCACCAAGCTTCTATTGGAACTCAACTTAATAATCAGTTCTTCCCAAATACAACTGGAAGATCTTGTGTACAGGATCTTAATTTTGCACAGGTTTACTGA

mRNA sequence

ATGGCTTATGTTAGTCAGAAGCCTGCATATGGCATTAAGAATCTCCATAAAAACAGATCAGCTGCTCGTGATGTGTGGTCATTTGATAAAATATCATGGGACGTAGAAGGTTGCTATCTTAGTCCTTTTAATATACTTCAGTGTTATCTTGAAAGCACTCTTCAGGTTGGTCTTGTTCATGAGATCATTGGAAATGGCTCTGAGGCTGAAACACTTTTAATGTGGGGAAAAAGTATCTCCTGCTTGCAAAGCTTGCCACTGTTTGAAGTTGCTTTCTCTTCTGCTTTAGGAAAATTATATAGAAAGAAAGAGTTGTGGAGTTCATCACAAAAGGAACTTGAAGGAGCCAAACAGATTTTAAAGGATAGCATTACTTCTTGTTTGAAGTGCAGATTGGTGCTTGAAGTAAAAGTTCATCAACATCTTGGAGATCTGTCCCGCGATATGTATGTAAATGCTAAAGGGATAATATCTGAAGAGCGATTAATAAATGCAGAAGGGTTTTATAATTTAGCTCTTGAGAAACTAAATCTTTCTACATGGAAAAATTCTATTAGTGGTATAGATGAAGAAACATTTCTTTCATCTTTGACAATTCAAGTGGAAAGGCCAAAGGACAAAAGGGATGGTAAAAAGGCTAAAAAAATAACAAATGCTCCCAAGTCTTTCCAAATGGACCAGTGCGTAAATCCTCGGAGTAATATGAGGTTGACTCGCTCTAGATGTCGGAAGATTCAGGGCCAAAGTGCAAGCAATTCCAATGATGTAGAAGTTGACCTTTCTCAATCACACTTGCAAGTGAATAGTTGCACACAAGGGAGAGAAGCATCATGCAAAAATGGGAAAGTTGGATGTTGGCAATGTCTACCAATGGAACTAATTGAAGCCGGACAAATGAACAATTTCATATATCTCAAGTGGGAGTTTGTTCGTAGACGGCTTGTGTTAAAACAGCTTTCTGGCATAGGTAAGTGCTCAGAGATGCGTGGTCAAATTCATCAAACACATGAAACTATTTTGAAAAGCATGTCGTTCATAGTAAGCAGAAATCTCTTCTTTCAAGAGCACTATGCGATTGAACCCACAGTTTTGCTTGAGTTAATTGGAAAGGAGGTCCATGGAGATGTGTTTGCTGTTGAGAGGGCATCGGTTCTCTATGAAATTTGCTGGTTCTCTTTGAAGAATACAATATGCAAATACCAAGTATGTATAGTTAATCTATGGATTATTTGTTGTCCTTTGTCTGAGGTTCACTCTGAAACATTGGTTTATTGGCTGAGGGTTGCCCTTGTACTTTGCTGTGAGGTTCCCGTACTTTTTCAAAAGGTTTCAAGATTGCTTGCTGTTATGCATGTAATTTCTTCAACAAGTGAGCTATTTTCTTTGGCATCTTCCAACACAATACTAGCAGACAGTCACTGGGCTTCATATTTTCACCAAGCTTCTATTGGAACTCAACTTAATAATCAGTTCTTCCCAAATACAACTGGAAGATCTTGTGTACAGGATCTTAATTTTGCACAGGTTTACTGA

Coding sequence (CDS)

ATGGCTTATGTTAGTCAGAAGCCTGCATATGGCATTAAGAATCTCCATAAAAACAGATCAGCTGCTCGTGATGTGTGGTCATTTGATAAAATATCATGGGACGTAGAAGGTTGCTATCTTAGTCCTTTTAATATACTTCAGTGTTATCTTGAAAGCACTCTTCAGGTTGGTCTTGTTCATGAGATCATTGGAAATGGCTCTGAGGCTGAAACACTTTTAATGTGGGGAAAAAGTATCTCCTGCTTGCAAAGCTTGCCACTGTTTGAAGTTGCTTTCTCTTCTGCTTTAGGAAAATTATATAGAAAGAAAGAGTTGTGGAGTTCATCACAAAAGGAACTTGAAGGAGCCAAACAGATTTTAAAGGATAGCATTACTTCTTGTTTGAAGTGCAGATTGGTGCTTGAAGTAAAAGTTCATCAACATCTTGGAGATCTGTCCCGCGATATGTATGTAAATGCTAAAGGGATAATATCTGAAGAGCGATTAATAAATGCAGAAGGGTTTTATAATTTAGCTCTTGAGAAACTAAATCTTTCTACATGGAAAAATTCTATTAGTGGTATAGATGAAGAAACATTTCTTTCATCTTTGACAATTCAAGTGGAAAGGCCAAAGGACAAAAGGGATGGTAAAAAGGCTAAAAAAATAACAAATGCTCCCAAGTCTTTCCAAATGGACCAGTGCGTAAATCCTCGGAGTAATATGAGGTTGACTCGCTCTAGATGTCGGAAGATTCAGGGCCAAAGTGCAAGCAATTCCAATGATGTAGAAGTTGACCTTTCTCAATCACACTTGCAAGTGAATAGTTGCACACAAGGGAGAGAAGCATCATGCAAAAATGGGAAAGTTGGATGTTGGCAATGTCTACCAATGGAACTAATTGAAGCCGGACAAATGAACAATTTCATATATCTCAAGTGGGAGTTTGTTCGTAGACGGCTTGTGTTAAAACAGCTTTCTGGCATAGGTAAGTGCTCAGAGATGCGTGGTCAAATTCATCAAACACATGAAACTATTTTGAAAAGCATGTCGTTCATAGTAAGCAGAAATCTCTTCTTTCAAGAGCACTATGCGATTGAACCCACAGTTTTGCTTGAGTTAATTGGAAAGGAGGTCCATGGAGATGTGTTTGCTGTTGAGAGGGCATCGGTTCTCTATGAAATTTGCTGGTTCTCTTTGAAGAATACAATATGCAAATACCAAGTATGTATAGTTAATCTATGGATTATTTGTTGTCCTTTGTCTGAGGTTCACTCTGAAACATTGGTTTATTGGCTGAGGGTTGCCCTTGTACTTTGCTGTGAGGTTCCCGTACTTTTTCAAAAGGTTTCAAGATTGCTTGCTGTTATGCATGTAATTTCTTCAACAAGTGAGCTATTTTCTTTGGCATCTTCCAACACAATACTAGCAGACAGTCACTGGGCTTCATATTTTCACCAAGCTTCTATTGGAACTCAACTTAATAATCAGTTCTTCCCAAATACAACTGGAAGATCTTGTGTACAGGATCTTAATTTTGCACAGGTTTACTGA

Protein sequence

MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVHEIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQILKDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLSTWKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRSRCRKIQGQSASNSNDVEVDLSQSHLQVNSCTQGREASCKNGKVGCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSMSFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVCIVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLASSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQVY
Homology
BLAST of IVF0014283 vs. ExPASy Swiss-Prot
Match: Q5IBC5 (Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 3.7e-88
Identity = 205/532 (38.53%), Postives = 284/532 (53.38%), Query Frame = 0

Query: 4    VSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVHEII 63
            +S+   + IKN    R  A D W      WD+  CYLSP+++LQCYLESTLQVG+++E+I
Sbjct: 1054 ISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELI 1113

Query: 64   GNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL--K 123
            GNG EAET+L WGK+ SC QSL  F VAFSSALG LY KK+    ++KEL+ AK+IL   
Sbjct: 1114 GNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIAN 1173

Query: 124  DSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLSTW 183
                SC+KC+L LEV + + LGD+SR        +   +  ++AE  ++ AL K   S W
Sbjct: 1174 QRDFSCVKCKLKLEVTLDKQLGDISRK---QIDRVSQTDGFLHAESLFSAALGKFCCSAW 1233

Query: 184  KNSISGIDEETF----------------LSSLTIQVERPKDKRDGKKAKKITNAPKSFQM 243
            K+ I    EE                   S   + ++ P   R  ++  +      S   
Sbjct: 1234 KSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQ 1293

Query: 244  DQCVNPRSNMRLTRSRCRKIQGQSASNSNDVEVDLSQSHLQVNSCTQGRE------ASCK 303
            D    P S  RLTRS    ++ Q  + SN  EV   + +L   S     E      ++  
Sbjct: 1294 DLISEPTS--RLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNAL 1353

Query: 304  NGKVGCW-----QCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQ 363
             G   C+     QCL  E+ E+G +NN + LKWE   R+L    L  +GKC    G+IH 
Sbjct: 1354 PGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHL 1413

Query: 364  THETILKSMSFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK 423
             HE +L S+S +      +  H     + LLE IGKEV  DVFAV+RA +LY +CW +L+
Sbjct: 1414 AHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLR 1473

Query: 424  NTICKYQVCIVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVIS 483
            N  C+          ICC L  +    LV WL +A VL  EVP+LFQKVSRLLA ++++S
Sbjct: 1474 NYHCRKSRS------ICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLS 1533

Query: 484  STSELFSLASSNTILADSHWASYFHQASIGTQLNNQFFPNTTGR---SCVQD 504
            S++  F+  S    L+ SHW S+FHQAS+GT L+  F  N + +    C+ D
Sbjct: 1534 SSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSD 1572

BLAST of IVF0014283 vs. ExPASy TrEMBL
Match: A0A5A7VCK1 (Separase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00310 PE=4 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.1e-279
Identity = 497/525 (94.67%), Postives = 498/525 (94.86%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNHIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYK-- 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 YANTKIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 509
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. ExPASy TrEMBL
Match: A0A1S4DV87 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.5e-277
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYK-- 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 YANTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 509
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. ExPASy TrEMBL
Match: A0A1S3BCF1 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.5e-277
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1078 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1137

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1138 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1197

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1198 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1257

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1258 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1317

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1318 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1377

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1378 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1437

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1438 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYK-- 1497

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1498 YANTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1557

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 509
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1558 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1596

BLAST of IVF0014283 vs. ExPASy TrEMBL
Match: A0A1S3BCW7 (Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.5e-277
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYK-- 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 YANTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 509
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. ExPASy TrEMBL
Match: A0A0A0KDL0 (Separase OS=Cucumis sativus OX=3659 GN=Csa_6G364113 PE=4 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.4e-257
Identity = 461/525 (87.81%), Postives = 475/525 (90.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAY+SQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFN+LQCYLESTLQVGLVH
Sbjct: 1166 MAYISQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVH 1225

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWS +QKELE AKQIL
Sbjct: 1226 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSLAQKELERAKQIL 1285

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQ LGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST
Sbjct: 1286 KDSITSCLKCRLVLEVKVHQQLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 1345

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISG+DEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNP+SNMRLTRS
Sbjct: 1346 WKNSISGLDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPQSNMRLTRS 1405

Query: 241  RCRKIQGQSASNSNDVEVDL-----------------SQSHLQVNSCTQGREASCKNGKV 300
            R R IQGQSASNSND +VDL                  QSHLQV+ CTQG EASCKNGKV
Sbjct: 1406 RYRTIQGQSASNSNDEKVDLPVHPKNNIPDISSALGQKQSHLQVSCCTQGSEASCKNGKV 1465

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSG+GK SE+RGQIHQTHETILKSM
Sbjct: 1466 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGLGKISEIRGQIHQTHETILKSM 1525

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SF+VSRNLFFQ HY IEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    +Y+  
Sbjct: 1526 SFVVSRNLFFQAHYVIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----RYKYA 1585

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
                 IICCPLS+V SETLV WLRVALVLCCEVP     VSRLLAVMHVISSTSELFSLA
Sbjct: 1586 TTK--IICCPLSQVDSETLVSWLRVALVLCCEVP-----VSRLLAVMHVISSTSELFSLA 1645

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 509
            SSNTILADSHW SYFHQAS+GTQ NNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1646 SSNTILADSHWVSYFHQASVGTQFNNQFFPNTTGRSCVQDLNFAQ 1679

BLAST of IVF0014283 vs. NCBI nr
Match: KAA0064727.1 (separase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 497/525 (94.67%), Postives = 498/525 (94.86%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNHIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYKYA 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 --NTKIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 508
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. NCBI nr
Match: XP_016899899.1 (PREDICTED: separase isoform X1 [Cucumis melo])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYKYA 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 --NTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 508
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. NCBI nr
Match: XP_008445507.1 (PREDICTED: separase isoform X3 [Cucumis melo])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1079 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1138

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1139 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1198

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1199 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1258

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1259 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1318

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1319 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1378

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1379 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1438

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1439 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYKYA 1498

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1499 --NTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1558

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 508
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1559 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1597

BLAST of IVF0014283 vs. NCBI nr
Match: XP_008445506.1 (PREDICTED: separase isoform X2 [Cucumis melo])

HSP 1 Score: 965 bits (2494), Expect = 0.0
Identity = 494/525 (94.10%), Postives = 496/525 (94.48%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAYVSQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH
Sbjct: 1078 MAYVSQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 1137

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL
Sbjct: 1138 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 1197

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERL+NAEGFYNLALEKLNLST
Sbjct: 1198 KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLMNAEGFYNLALEKLNLST 1257

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS
Sbjct: 1258 WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 1317

Query: 241  RCRKIQGQSASNSNDVEVDLS-----------------QSHLQVNSCTQGREASCKNGKV 300
            RCRKIQGQSASNSNDVEVDLS                 QSHLQVNSCTQGREASCKNGKV
Sbjct: 1318 RCRKIQGQSASNSNDVEVDLSVHLKNNIPDISSALGQKQSHLQVNSCTQGREASCKNGKV 1377

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM
Sbjct: 1378 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 1437

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    KY+  
Sbjct: 1438 SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----KYKYA 1497

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
              N  IICCPLSEVHSETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1498 --NTKIICCPLSEVHSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1557

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 508
            SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1558 SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 1596

BLAST of IVF0014283 vs. NCBI nr
Match: XP_011657384.1 (separase isoform X2 [Cucumis sativus] >KAE8647231.1 hypothetical protein Csa_019070 [Cucumis sativus])

HSP 1 Score: 914 bits (2363), Expect = 2.29e-311
Identity = 466/525 (88.76%), Postives = 480/525 (91.43%), Query Frame = 0

Query: 1    MAYVSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVH 60
            MAY+SQKPAYGIKNL KNRSAARDVWSFDKISWDVEGCYLSPFN+LQCYLESTLQVGLVH
Sbjct: 1078 MAYISQKPAYGIKNLQKNRSAARDVWSFDKISWDVEGCYLSPFNVLQCYLESTLQVGLVH 1137

Query: 61   EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL 120
            EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWS +QKELE AKQIL
Sbjct: 1138 EIIGNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSLAQKELERAKQIL 1197

Query: 121  KDSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 180
            KDSITSCLKCRLVLEVKVHQ LGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST
Sbjct: 1198 KDSITSCLKCRLVLEVKVHQQLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLST 1257

Query: 181  WKNSISGIDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPRSNMRLTRS 240
            WKNSISG+DEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNP+SNMRLTRS
Sbjct: 1258 WKNSISGLDEETFLSSLTIQVERPKDKRDGKKAKKITNAPKSFQMDQCVNPQSNMRLTRS 1317

Query: 241  RCRKIQGQSASNSNDVEVDL-----------------SQSHLQVNSCTQGREASCKNGKV 300
            R R IQGQSASNSND +VDL                  QSHLQV+ CTQG EASCKNGKV
Sbjct: 1318 RYRTIQGQSASNSNDEKVDLPVHPKNNIPDISSALGQKQSHLQVSCCTQGSEASCKNGKV 1377

Query: 301  GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQTHETILKSM 360
            GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSG+GK SE+RGQIHQTHETILKSM
Sbjct: 1378 GCWQCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGLGKISEIRGQIHQTHETILKSM 1437

Query: 361  SFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLKNTICKYQVC 420
            SF+VSRNLFFQ HY IEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK    +Y+  
Sbjct: 1438 SFVVSRNLFFQAHYVIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK----RYKYA 1497

Query: 421  IVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 480
                 IICCPLS+V SETLV WLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA
Sbjct: 1498 TTK--IICCPLSQVDSETLVSWLRVALVLCCEVPVLFQKVSRLLAVMHVISSTSELFSLA 1557

Query: 481  SSNTILADSHWASYFHQASIGTQLNNQFFPNTTGRSCVQDLNFAQ 508
            SSNTILADSHW SYFHQAS+GTQ NNQFFPNTTGRSCVQDLNFAQ
Sbjct: 1558 SSNTILADSHWVSYFHQASVGTQFNNQFFPNTTGRSCVQDLNFAQ 1596

BLAST of IVF0014283 vs. TAIR 10
Match: AT4G22970.1 (homolog of separase )

HSP 1 Score: 327.0 bits (837), Expect = 2.7e-89
Identity = 205/532 (38.53%), Postives = 284/532 (53.38%), Query Frame = 0

Query: 4    VSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVHEII 63
            +S+   + IKN    R  A D W      WD+  CYLSP+++LQCYLESTLQVG+++E+I
Sbjct: 1054 ISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELI 1113

Query: 64   GNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL--K 123
            GNG EAET+L WGK+ SC QSL  F VAFSSALG LY KK+    ++KEL+ AK+IL   
Sbjct: 1114 GNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIAN 1173

Query: 124  DSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLSTW 183
                SC+KC+L LEV + + LGD+SR        +   +  ++AE  ++ AL K   S W
Sbjct: 1174 QRDFSCVKCKLKLEVTLDKQLGDISRK---QIDRVSQTDGFLHAESLFSAALGKFCCSAW 1233

Query: 184  KNSISGIDEETF----------------LSSLTIQVERPKDKRDGKKAKKITNAPKSFQM 243
            K+ I    EE                   S   + ++ P   R  ++  +      S   
Sbjct: 1234 KSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQ 1293

Query: 244  DQCVNPRSNMRLTRSRCRKIQGQSASNSNDVEVDLSQSHLQVNSCTQGRE------ASCK 303
            D    P S  RLTRS    ++ Q  + SN  EV   + +L   S     E      ++  
Sbjct: 1294 DLISEPTS--RLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNAL 1353

Query: 304  NGKVGCW-----QCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQ 363
             G   C+     QCL  E+ E+G +NN + LKWE   R+L    L  +GKC    G+IH 
Sbjct: 1354 PGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHL 1413

Query: 364  THETILKSMSFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK 423
             HE +L S+S +      +  H     + LLE IGKEV  DVFAV+RA +LY +CW +L+
Sbjct: 1414 AHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLR 1473

Query: 424  NTICKYQVCIVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVIS 483
            N  C+          ICC L  +    LV WL +A VL  EVP+LFQKVSRLLA ++++S
Sbjct: 1474 NYHCRKSRS------ICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLS 1533

Query: 484  STSELFSLASSNTILADSHWASYFHQASIGTQLNNQFFPNTTGR---SCVQD 504
            S++  F+  S    L+ SHW S+FHQAS+GT L+  F  N + +    C+ D
Sbjct: 1534 SSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSD 1572

BLAST of IVF0014283 vs. TAIR 10
Match: AT4G22970.2 (homolog of separase )

HSP 1 Score: 327.0 bits (837), Expect = 2.7e-89
Identity = 205/532 (38.53%), Postives = 284/532 (53.38%), Query Frame = 0

Query: 4    VSQKPAYGIKNLHKNRSAARDVWSFDKISWDVEGCYLSPFNILQCYLESTLQVGLVHEII 63
            +S+   + IKN    R  A D W      WD+  CYLSP+++LQCYLESTLQVG+++E+I
Sbjct: 1054 ISEIRTFSIKNFQVYRLLATDFWPCGNFLWDINRCYLSPWSVLQCYLESTLQVGILNELI 1113

Query: 64   GNGSEAETLLMWGKSISCLQSLPLFEVAFSSALGKLYRKKELWSSSQKELEGAKQIL--K 123
            GNG EAET+L WGK+ SC QSL  F VAFSSALG LY KK+    ++KEL+ AK+IL   
Sbjct: 1114 GNGLEAETILSWGKAFSCSQSLFPFVVAFSSALGNLYHKKQCLDLAEKELQNAKEILIAN 1173

Query: 124  DSITSCLKCRLVLEVKVHQHLGDLSRDMYVNAKGIISEERLINAEGFYNLALEKLNLSTW 183
                SC+KC+L LEV + + LGD+SR        +   +  ++AE  ++ AL K   S W
Sbjct: 1174 QRDFSCVKCKLKLEVTLDKQLGDISRK---QIDRVSQTDGFLHAESLFSAALGKFCCSAW 1233

Query: 184  KNSISGIDEETF----------------LSSLTIQVERPKDKRDGKKAKKITNAPKSFQM 243
            K+ I    EE                   S   + ++ P   R  ++  +      S   
Sbjct: 1234 KSCIRSHGEEIAEEIVIDRNGGEGLGHNSSKTKLSIKEPPGNRGSRRGGRANKTCLSKDQ 1293

Query: 244  DQCVNPRSNMRLTRSRCRKIQGQSASNSNDVEVDLSQSHLQVNSCTQGRE------ASCK 303
            D    P S  RLTRS    ++ Q  + SN  EV   + +L   S     E      ++  
Sbjct: 1294 DLISEPTS--RLTRSMRHSLREQCQNRSNVPEVVSKKPNLCDRSVGSRGERVLLDTSNAL 1353

Query: 304  NGKVGCW-----QCLPMELIEAGQMNNFIYLKWEFVRRRLVLKQLSGIGKCSEMRGQIHQ 363
             G   C+     QCL  E+ E+G +NN + LKWE   R+L    L  +GKC    G+IH 
Sbjct: 1354 PGFCICYKEKRQQCLSEEVTESGSLNNLVSLKWELCHRKLASSILVSLGKCLGDSGRIHL 1413

Query: 364  THETILKSMSFIVSRNLFFQEHYAIEPTVLLELIGKEVHGDVFAVERASVLYEICWFSLK 423
             HE +L S+S +      +  H     + LLE IGKEV  DVFAV+RA +LY +CW +L+
Sbjct: 1414 AHEALLHSISVLFKST--WSSHNQPSVSQLLEFIGKEVTRDVFAVDRAIILYNLCWLNLR 1473

Query: 424  NTICKYQVCIVNLWIICCPLSEVHSETLVYWLRVALVLCCEVPVLFQKVSRLLAVMHVIS 483
            N  C+          ICC L  +    LV WL +A VL  EVP+LFQKVSRLLA ++++S
Sbjct: 1474 NYHCRKSRS------ICCDLFHIPFTKLVSWLMLAFVLSGEVPILFQKVSRLLASLYLLS 1533

Query: 484  STSELFSLASSNTILADSHWASYFHQASIGTQLNNQFFPNTTGR---SCVQD 504
            S++  F+  S    L+ SHW S+FHQAS+GT L+  F  N + +    C+ D
Sbjct: 1534 SSNSEFTFESDGNELSASHWVSFFHQASLGTHLSYHFISNLSQKHKSQCLSD 1572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5IBC53.7e-8838.53Separase OS=Arabidopsis thaliana OX=3702 GN=ESP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7VCK13.1e-27994.67Separase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00310 PE=4 S... [more]
A0A1S4DV876.5e-27794.10Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1[more]
A0A1S3BCF16.5e-27794.10Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1[more]
A0A1S3BCW76.5e-27794.10Separase OS=Cucumis melo OX=3656 GN=LOC103488503 PE=4 SV=1[more]
A0A0A0KDL03.4e-25787.81Separase OS=Cucumis sativus OX=3659 GN=Csa_6G364113 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
KAA0064727.10.094.67separase isoform X1 [Cucumis melo var. makuwa][more]
XP_016899899.10.094.10PREDICTED: separase isoform X1 [Cucumis melo][more]
XP_008445507.10.094.10PREDICTED: separase isoform X3 [Cucumis melo][more]
XP_008445506.10.094.10PREDICTED: separase isoform X2 [Cucumis melo][more]
XP_011657384.12.29e-31188.76separase isoform X2 [Cucumis sativus] >KAE8647231.1 hypothetical protein Csa_019... [more]
Match NameE-valueIdentityDescription
AT4G22970.12.7e-8938.53homolog of separase [more]
AT4G22970.22.7e-8938.53homolog of separase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR12792:SF1BNAC04G05300D PROTEINcoord: 8..501
IPR005314Peptidase C50, separasePANTHERPTHR12792EXTRA SPINDLE POLES 1-RELATEDcoord: 8..501

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0014283.1IVF0014283.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098813 nuclear chromosome segregation
biological_process GO:0000280 nuclear division
biological_process GO:0006508 proteolysis
cellular_component GO:0005634 nucleus
molecular_function GO:0004197 cysteine-type endopeptidase activity