IVF0014169 (gene) Melon (IVF77) v1

Overview
NameIVF0014169
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionflavonol synthase/flavanone 3-hydroxylase
Locationchr03: 12744648 .. 12745839 (-)
RNA-Seq ExpressionIVF0014169
SyntenyIVF0014169
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTCGGAAGGAATTCTTCGAGCTCCCACCTGAAGAGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGTAACGAAATCAATAAATTCCTCCGAGTTCTTTCTTAATAATTTGTTATTGTACTCTGTTTTGATTTTTGGGTGCACGTCTGTCCGAGAATGCAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGGTAAAGTGTTACTAGAATTCAAGAGATGTTTGATTTTTTGAGTTGCGTGGGAAATGGCTGAATTAGAAGTTTGTTGTGTTGTGGGGGCAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGTAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

mRNA sequence

ATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGTAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Coding sequence (CDS)

ATGGATGTTGGGAGAGTCCAGGAAGTTGCTTCATCTCTTCTTTTCTCCGGCACTCTCCCCTCCGAGTTTGTCCGACCAGAGACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATTGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCTCTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTCGAAGGCTACGGAACTAAGCTCGAAAAAGAAATCGATGGAAAAAGAGGCTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAGCGAATGAAGAGTACAGAAAGAATTTACATGGGGTTGTGGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAATTGGTGTATTTACTGAAAATCAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCGTTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCTCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCCGTGTTCTTGGAGCCACCGCCGGAGTTTGTAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAAATATAAGACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Protein sequence

MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAKEIYAKPPASKSVEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFVVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Homology
BLAST of IVF0014169 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 1.6e-137
Identity = 224/336 (66.67%), Postives = 267/336 (79.46%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ +AS      T+PSE++R E EQP  TT+ G VL VPVID  +PD++ ++  I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQ-------------AKEIYAKPPASKSVEGYGTKL 120
            DAS  WG+FQ+INH IP+EAI  LQ              KE+ AK P S  +EGYGT L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 EKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSL 180
           +KE++GK+GWVDHLFH IWPPS++NY +WP+NPPSYR ANEEY K +  VV+++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQ 240
           GLGLEGHE+ E  GGDE+VYLLKINYYPPCPRPDLALGV AHTDMS +TILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPE 300
            F+DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV+HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FVVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
             VGP PKL+ E NP K+KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

BLAST of IVF0014169 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 482.6 bits (1241), Expect = 3.4e-135
Identity = 227/323 (70.28%), Postives = 262/323 (81.11%), Query Frame = 0

Query: 17  GTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQ 76
           GT+P+EF+R E EQPGITTV G+VL VP+IDF++PD++ ++  I +AS NWGM+QI+NH 
Sbjct: 15  GTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHD 74

Query: 77  IPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLEKEID----GKRGWVDH 136
           IP+E I+ LQA            KE YAKPP S S+EGYGTKL KEI      K+GWVD+
Sbjct: 75  IPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDN 134

Query: 137 LFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLGLGLEGHELKERV 196
           LF+ IWPPS +NY FWP+NPPSYR ANEEY K+LH VVEKLFR LSLGLGLEG ELK+  
Sbjct: 135 LFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAA 194

Query: 197 GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQAFRDGHWYDVNYI 256
           GGD L YLLKINYYPPCPRPDLALGV AHTDMS VTILVPN+VQGLQA +DG WYDV YI
Sbjct: 195 GGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYI 254

Query: 257 PNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFVVGPHPKLVDEE 316
           PNALVIHIGDQ+EI+SNGKY SV HRTTVNK+KTRISWPVFLEPP + VVGPHP+LV+  
Sbjct: 255 PNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAV 314

Query: 317 NPAKYKTKKYSEYAYCKLNKIPQ 324
           N  KYKTKKY +Y YCK+NK+PQ
Sbjct: 315 NQPKYKTKKYGDYVYCKINKLPQ 337

BLAST of IVF0014169 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 481.5 bits (1238), Expect = 7.5e-135
Identity = 221/335 (65.97%), Postives = 269/335 (80.30%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQE+AS      T+P+E++R E EQP I+TV G VL VPVID ++ D+  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQ------------AKEIYAKPPASKSVEGYGTKLE 120
            +AS  WG+FQ++NH IPNE I  LQ             KE+ AKP  S+S+EGYGT+L+
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFH IWPPS+INY FWP+NPP+YR ANEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           L LE +  K+  GGD+LVYL+KINYYPPCPRPDLALGV AHTDMSA+T+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           ++DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV+HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
            VGP PKLV+EENPAK+KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

BLAST of IVF0014169 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.9e-133
Identity = 219/337 (64.99%), Postives = 268/337 (79.53%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDF--TNPDDDSVLT 60
           M+V RVQ ++       T+PSE++R E+EQP +TT++G VL VPVID   +N  +++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIHDASLNWGMFQIINHQIPNEAITHL------------QAKEIYAKPPASKSVEGYGTK 120
            I +AS  WG+FQ++NH IP++AI  L            Q KE+ AKP   + VEGYGTK
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLS 180
           L+KE+ GK+GWVDHLFHIIWP S++NYNFWP NPP YR ANEEY   L GVV+KLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGL 240
           LG+GLE HELK+  GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMSA+TILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPP 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV+HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFVVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
           E + GP  KL+ +ENPAK+KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

BLAST of IVF0014169 vs. ExPASy Swiss-Prot
Match: Q41452 (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 3.6e-129
Identity = 216/338 (63.91%), Postives = 263/338 (77.81%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDD--SVLT 60
           M+V RVQ ++S      T+PSE++R E EQP  TT++G VL VPVID +N DDD   ++ 
Sbjct: 15  MEVARVQAISSITKCMDTIPSEYIRSENEQPAATTLQGVVLEVPVIDISNVDDDEEKLVK 74

Query: 61  AIHDASLNWGMFQIINHQIPNEAITHLQ-------------AKEIYAKPPASKSVEGYGT 120
            I +AS  WG+FQ+INH IP+E I +LQ              KE+ AK P ++S+EGYGT
Sbjct: 75  EIVEASKEWGIFQVINHGIPDEVIENLQKVGKEFFEEVPQEEKELIAKKPGAQSLEGYGT 134

Query: 121 KLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSL 180
            L+KEI+GK+GWVDHLFH IWPPS+INY +WP+NPPSYR ANEEY K L  V + +FRSL
Sbjct: 135 SLQKEIEGKKGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKWLRKVADGIFRSL 194

Query: 181 SLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQG 240
           SLGLGLEGHE+ E  G +++VY+LKINYYPPCPRPDLALGV AHTDMS +T+LVPNEV  
Sbjct: 195 SLGLGLEGHEMMEAAGSEDIVYMLKINYYPPCPRPDLALGVVAHTDMSYITLLVPNEV-- 254

Query: 241 LQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPP 300
            Q F+DGHWYDVNYIPNA+++HIGDQ+EILSNGKYKSV+HRTTVNK KTR+SWPVFLEP 
Sbjct: 255 -QVFKDGHWYDVNYIPNAIIVHIGDQVEILSNGKYKSVYHRTTVNKYKTRMSWPVFLEPS 314

Query: 301 PEFVVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
            E  VGP P L++E NP K+KTKKY +Y YCKLNK+PQ
Sbjct: 315 SEHEVGPIPNLINEANPPKFKTKKYKDYVYCKLNKLPQ 349

BLAST of IVF0014169 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 7.2e-189
Identity = 323/335 (96.42%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNEAITHLQA            KEIYAKPPASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
           VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 7.2e-189
Identity = 323/335 (96.42%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNEAITHLQA            KEIYAKPPASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
           VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 2.2e-182
Identity = 310/335 (92.54%), Postives = 318/335 (94.93%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNEAITHLQA            KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
            VGPHPKLVDE+ PA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 7.7e-167
Identity = 277/335 (82.69%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNEAI HLQA            KE+YAKPP SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DG++GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
            VGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.3e-166
Identity = 276/335 (82.39%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG++P+EFVRP+ EQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNEAI HLQA            KE+YAKPP SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYRVANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELK+  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 324
            VGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 663 bits (1710), Expect = 2.88e-240
Identity = 323/335 (96.42%), Postives = 323/335 (96.42%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNEAITHLQA            KEIYAKPPASKSVEGYGTKLE
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 323
           VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 641 bits (1654), Expect = 9.90e-232
Identity = 310/335 (92.54%), Postives = 318/335 (94.93%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
           HDASLNWGMFQIINHQIPNEAITHLQA            KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR ANEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 323
            VGPHPKLVDE+ PA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 610 bits (1574), Expect = 1.55e-219
Identity = 292/334 (87.43%), Postives = 309/334 (92.51%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS+L+S TLP+EFVR ETEQPGITTVKGQVLAVPVIDFT+PDDDSVL AI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
            DAS NWGMFQIINH+IPNEAITHLQA            KE+YAKPP SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHIIWPPS+INYNFWPQNPPSYR ANEEY K+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKER GG+ELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDG WYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 322
            VGPHPKLVDE+NP KYKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

BLAST of IVF0014169 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 591 bits (1523), Expect = 9.14e-212
Identity = 278/335 (82.99%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNEAI HLQA            KE+YAKPP SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 323
            VGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591 bits (1523), Expect = 9.14e-212
Identity = 278/335 (82.99%), Postives = 303/335 (90.45%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQ++ASS LFSG+LP+EFVRP+TEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQA------------KEIYAKPPASKSVEGYGTKLE 120
            DAS NWGMFQIINH IPNEAI HLQA            KE+YAKPP SKSVEGYGTKL+
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR ANEEYRK+LHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS +TILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 323
            VGPHPKLVD++NP KYKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

BLAST of IVF0014169 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 417.9 bits (1073), Expect = 7.3e-117
Identity = 191/334 (57.19%), Postives = 246/334 (73.65%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ+++SS L +  +P EF+R E EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQ------------AKEIYAKPPASKSVEGYGTKLE 120
             AS  WG+FQ++NH IP E I  LQ             KE  AKP  SK +EGYGTKL+
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  ++  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S +T+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 323
           +VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of IVF0014169 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 417.9 bits (1073), Expect = 7.3e-117
Identity = 191/334 (57.19%), Postives = 246/334 (73.65%), Query Frame = 0

Query: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+V RVQ+++SS L +  +P EF+R E EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQ------------AKEIYAKPPASKSVEGYGTKLE 120
             AS  WG+FQ++NH IP E I  LQ             KE  AKP  SK +EGYGTKL+
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  ++  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S +T+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIP 323
           +VGP P+L  ++NP K+K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

BLAST of IVF0014169 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 312.0 bits (798), Expect = 5.6e-85
Identity = 145/294 (49.32%), Postives = 194/294 (65.99%), Query Frame = 0

Query: 41  LAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHL------------QAK 100
           L +PVID +NPD++ V +A+  AS  WG+FQ++NH IP E I  L              K
Sbjct: 12  LDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETEK 71

Query: 101 EIYAKPPASKSVEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANE 160
           E  AKP  S  +EGY TK +K+++G+  WVDHLFH IWPPS +N+ FWP+NPP Y   NE
Sbjct: 72  EAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNE 131

Query: 161 EYRKNLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPA 220
           EY  ++  + EK+   LS GLGL    LKE +GG+ + YL+KINYYPPCP P+L +G P 
Sbjct: 132 EYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAPD 191

Query: 221 HTDMSAVTILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTT 280
           HTD++ +T+LV NE  GLQAF+D  W D  Y  + +++ IGDQ   +SNGKYKSV HR  
Sbjct: 192 HTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRAK 251

Query: 281 VNKEKTRISWPVFLEPPPEFVVGPHPKLV-DEENPAKYKTKKYSEYAYCKLNKI 322
           ++KEKTRISWPVF+E   + V GP P+L+  +EN  K+K   Y +Y + KL K+
Sbjct: 252 MDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

BLAST of IVF0014169 vs. TAIR 10
Match: AT4G22880.1 (leucoanthocyanidin dioxygenase )

HSP 1 Score: 269.6 bits (688), Expect = 3.2e-72
Identity = 141/341 (41.35%), Postives = 205/341 (60.12%), Query Frame = 0

Query: 3   VGRVQEVASSLLFSGTLPSEFVRPETEQPGITTV-----KGQVLAVPVIDFTNPDDD--- 62
           V RV+ +A S + S  +P E++RP+ E   I  V     K     VP ID  N + D   
Sbjct: 4   VERVESLAKSGIIS--IPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEK 63

Query: 63  ---SVLTAIHDASLNWGMFQIINHQIPNEAITH------------LQAKEIYAKPPASKS 122
              + +  +  ASL+WG+  +INH IP + +              ++ KE YA   A+  
Sbjct: 64  IRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGK 123

Query: 123 VEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVE 182
           ++GYG+KL     G+  W D+ FH+ +P    + + WP+ P  Y  A  EY K L  +  
Sbjct: 124 IQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLAT 183

Query: 183 KLFRSLSLGLGLEGHELKERVGG-DELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTIL 242
           K+F++LS+GLGLE   L++ VGG +EL+  +KINYYP CP+P+LALGV AHTD+SA+T +
Sbjct: 184 KVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFI 243

Query: 243 VPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISW 302
           + N V GLQ F +G W     +P+++V+HIGD +EILSNGKYKS+ HR  VNKEK RISW
Sbjct: 244 LHNMVPGLQLFYEGKWVTAKCVPDSIVMHIGDTLEILSNGKYKSILHRGLVNKEKVRISW 303

Query: 303 PVFLEPPPE-FVVGPHPKLVDEENPAKYKTKKYSEYAYCKL 319
            VF EPP +  V+ P P++V  E+PAK+  + ++++   KL
Sbjct: 304 AVFCEPPKDKIVLKPLPEMVSVESPAKFPPRTFAQHIEHKL 342

BLAST of IVF0014169 vs. TAIR 10
Match: AT4G22880.2 (leucoanthocyanidin dioxygenase )

HSP 1 Score: 269.6 bits (688), Expect = 3.2e-72
Identity = 141/341 (41.35%), Postives = 205/341 (60.12%), Query Frame = 0

Query: 3   VGRVQEVASSLLFSGTLPSEFVRPETEQPGITTV-----KGQVLAVPVIDFTNPDDD--- 62
           V RV+ +A S + S  +P E++RP+ E   I  V     K     VP ID  N + D   
Sbjct: 4   VERVESLAKSGIIS--IPKEYIRPKEELESINDVFLEEKKEDGPQVPTIDLKNIESDDEK 63

Query: 63  ---SVLTAIHDASLNWGMFQIINHQIPNEAITH------------LQAKEIYAKPPASKS 122
              + +  +  ASL+WG+  +INH IP + +              ++ KE YA   A+  
Sbjct: 64  IRENCIEELKKASLDWGVMHLINHGIPADLMERVKKAGEEFFSLSVEEKEKYANDQATGK 123

Query: 123 VEGYGTKLEKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVE 182
           ++GYG+KL     G+  W D+ FH+ +P    + + WP+ P  Y  A  EY K L  +  
Sbjct: 124 IQGYGSKLANNASGQLEWEDYFFHLAYPEEKRDLSIWPKTPSDYIEATSEYAKCLRLLAT 183

Query: 183 KLFRSLSLGLGLEGHELKERVGG-DELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTIL 242
           K+F++LS+GLGLE   L++ VGG +EL+  +KINYYP CP+P+LALGV AHTD+SA+T +
Sbjct: 184 KVFKALSVGLGLEPDRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFI 243

Query: 243 VPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISW 302
           + N V GLQ F +G W     +P+++V+HIGD +EILSNGKYKS+ HR  VNKEK RISW
Sbjct: 244 LHNMVPGLQLFYEGKWVTAKCVPDSIVMHIGDTLEILSNGKYKSILHRGLVNKEKVRISW 303

Query: 303 PVFLEPPPE-FVVGPHPKLVDEENPAKYKTKKYSEYAYCKL 319
            VF EPP +  V+ P P++V  E+PAK+  + ++++   KL
Sbjct: 304 AVFCEPPKDKIVLKPLPEMVSVESPAKFPPRTFAQHIEHKL 342

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q075121.6e-13766.67Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ... [more]
Q9XHG23.4e-13570.28Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2... [more]
Q9M5477.5e-13565.97Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian... [more]
Q7XZQ61.9e-13364.99Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS... [more]
Q414523.6e-12963.91Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CHN57.2e-18996.42Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BCS77.2e-18996.42flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863... [more]
A0A0A0K8852.2e-18292.54Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
A0A6J1FDW37.7e-16782.69flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JY722.3e-16682.39flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_008445671.12.88e-24096.42PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.... [more]
XP_004140862.19.90e-23292.54flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet... [more]
XP_038885635.11.55e-21987.43flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida][more]
KAG7016505.19.14e-21282.99Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp... [more]
XP_023551148.19.14e-21282.99flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G08640.17.3e-11757.19flavonol synthase 1 [more]
AT5G08640.27.3e-11757.19flavonol synthase 1 [more]
AT5G63590.15.6e-8549.32flavonol synthase 3 [more]
AT4G22880.13.2e-7241.35leucoanthocyanidin dioxygenase [more]
AT4G22880.23.2e-7241.35leucoanthocyanidin dioxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 43..138
e-value: 2.0E-10
score: 41.4
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..321
e-value: 1.2E-86
score: 293.0
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 189..284
e-value: 2.5E-25
score: 88.9
NoneNo IPR availablePANTHERPTHR47990:SF36OS02G0767300 PROTEINcoord: 6..320
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 6..320
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..321
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 184..284
score: 14.531129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0014169.1IVF0014169.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051555 flavonol biosynthetic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0045431 flavonol synthase activity
molecular_function GO:0046872 metal ion binding