IVF0013556 (gene) Melon (IVF77) v1

Overview


Name	IVF0013556
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	subtilisin-like protease SBT2.3
Location	chr08: 390392 .. 395062 (-)
RNA-Seq Expression	IVF0013556
Synteny	IVF0013556

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATTATTTACTGTGCACGTCTACTATGTGTTGTTGTCTGTTTTGGGATGTTTGTATGCGCGTCTTGTCTGGATGAATTCGGTGATTCAACAGCTGTTTACATTGTAACTCTCAAGGAACCTCCTTCTGCTACTCATTATTATGGTCAGCTTAGACAAAATACCACTTCTTTTAGTACTTCTGGTGGATTAAGCATCCATAAAGCAAGGTACTTCTCCATCACTCCTTTCCCTTCTCTCATATACTTGCATTAAGCAGCTCTAAGCTCCTCGTTTCAGTGTTTTATTTGAAATATGATGAAAACTTGTTGTATAAGACATTTGCAATTACTGTATTTTTCTTAAAGCCCAACGTGGGCATATTTGTTCAAGCATGAGTTGTTGACATGCTCTCTAGTTGATATTGATTACTTTTTCTGGGGTTTGGAAGTTCGTCTTTCTTTCTCTGTCAAAGTTCTATTTTTTTTTCATCATTTGGTACTTGTATTGTGTTGGAAGAATACATAATTTGTGCGTGTACAGCTTTGTTGTATTTACTAATCTCCTATCTCTGTTTCTCAGTGAAGTTCCATGAAAACTATCCCACTACCTTGTAGAAACTATTGCTTTGCTAATTTCTTTTTAATGTATTGTCGGTGTTTTGTTCAATGTATTGTCGACGGGAAAATAATAATTACGGACTGAAATTATTGCTTGAAATTGTGATTGGCCCTAATGCTATGCCTTTTTGCTTCTGCTCTTCCTCCTGTTTAGTCTGTTTGATTATTTCACAGGTAGTATAATTGCCTTTTCTTTTTTAATTGTAATTTAAAGTCTACTGCTTGATGTTTATTTTATTTTATTTTATCTAGAAACAAATCAAGAAAGCATCAAAGATATAGATCTTACATAGCTCGAGTTCATGATTCATTGTTGAAGAAGGTCTTGAGGGGAGAGAAATATCTGAAGCTGTACAGTTACCATTTCTTGATCAATGGATTTGCTGTGCTTGTTACCGAAGAACAGGTACCTGTTTTGTAATTTTTTCTCTCTGTTTAGCATCATCAACATTGATGATTCCCCCACTTTGAACTGTGATGCTTTTTAATTGTTAGTGTTTACCTTATAAAATTTGTTCACAGCAATTACGTGCTGTCTAATAACACTTTGGCGTCTAAGAATCGAAATATTGTAGTCTAATAGGTTCATAACGTAGGTTTTGATTTCTGGTACTTCTAATTTGTAGGCAAATAAACTTTCAAAGAGGATAGAGGTGGCAAATGTTGTCATGGATTTCTCTGTTAGAACTGCTACGACCCATACTCCACAGTTCTTGGGCCTCCCGCAGGGAGCTTGGTCTCAAGATGGTGGCTTTGAATCTGCTGGAGCTGGGATTGTGATAGGGTTCATTGACACTGGCATTGATCCTTCGCACCCCAGTTTTGCTGATGATTTGACTGATAATCCGTTTCCCGTTCCGGCTCACTTCTCTGGAATTTGTGAGGTAACTCCAGATTTTCCTTCTGGTCTTGCAACCGGAAGCTTGTGGGAGCGCGCCATTTTGCAGCATCAGCTATAACAAGGGGAATATTTAATGCAACTCAGGATTATGCATCACCATTTGATGGTGATGGACATGGCACGTAAGTGTTTCTTTTATTTTCTGGAAGTTTGTGGATCAAATTATTATTTCTGTTGGTTCTTTTCCTTGTCAGCCATGACATCATAAACCCAACTCATTTTTGGTTGGAACTAAGAAGCTATGAAAATATTCATATGTATTATTTTAAATAAATTATATAGATATGTATTATTACTTTCCATAAAGCTAATATTGCCTTTAGAGAAAGGCTTTACAGAAGGTGATGAGAACATGATTTCTGGTTTGTTTGTTTGTTACATTTTCCTTGTGGAATCTAAATGAAGAGTGATAAAGTTTTAGGGAAGCTTAGATGATGGAAATGAAATTTGACTTTTATGCTCTTGAGGAGCACAAAAATGTTAACTTTGTTTTCAAGTTAGGGTTGGATGCATGTCTAGTTCCTTTTTCTTTGGAAAAAAATAGAGAAAAAGATTGCATTCCTAGTTATATCTTATGAATTTTTGCTTTCTGGAGGCCTCCCTTTTGTGGGTTTTTTCCTTTTGTATTCCCTTTATTCTGTCATTTTTTCTCAATGAAAGTTTGGTTATTCTTTTCTTTCAAAAAATACTATAATTTGTATTTCCCCTCTCTCCCACCCTCAGTTCTCTTCTTCTTTTCCCCTTCTTTTTTCCACTTTAGGTTCATCCTGGTGGATATTCACTTATTCCATTTCTTTGGATACATCTTTTCCCTTTCAGCTGCCTCCATACTATGTATTAATTTTAATGAAAATGGTGTGACCCTATTATCTGCAGGCACACAGCTTCAATTGCTGCTGGAAACCATGGCATTCCAGTCATAGTTGCTGGACATCACTTTGGAAATGCCAGTGGATGGCTCCTCGTTCACAGTAATTAATCTGGACTTAGGCTACAGTCGCTCATTGCTCCTAGCTATTATCTTTTTTTTTTTTCCTTTGATATGCTTGTTTCTTATTTTTTACTTAAAATACATGTTATAGAGCTCGAGTTTCATTTGCTTCCTTGTTTGAATGCTTGCTTATGGTTCTTTATTCAGTATTGCTGTTTACAAGGCACTGTACAAAAGCTTTGGAGGTTTTGCTGCTGATGTTGTTGCTGCAGTTGATCAGGTGTGTATGATATGCTTGATTGTTTTCCATTACAAAAGTTGTTTAGTTTGTTTCTGGCTCTGCAATGGGAGTTGGAGTTGAGAAGTTCTTGACGACTTGGTATCAAACTTCTGTATTTAGAATAAGTGTAAGCAGTTCAACTCTTGAGGATGCAGGATTACCTACTAAATAAACAAATGAGAATACAACTTTATTCTTTTGTGTTCTATTGTAATCTATGATGTCTTTTGTAGGCTGCTCAGGATGGGGTGGATATAATAAGTTTATCAATCACACCAATCGGCGTCCTCCTGGTATTGCAACGTTTTTTAATCCCATTGATATGGCGCTGCTCTCTGCTGTAAAGACTGGTATATTTGTTGTGCAAGCGGCTGGCAACACTGGACCAGCACCAAAGAGCATGTCTTCCTTCAGTCCATGGATCTTTACTGTTGGCGCTGCTTCTCACGATAGAAGCTATGCTAACTCTATAAGCCTTGGCAATAATGTCACCATCCCGGGAGTCGGACTTGCACGTGAGTTTCTGCTGTCTTCTCTGTTTGTACTACTCTTCCGCAGGGATTTCATTCTTATAAGAAAGCAGAGGGAACTGAATTTGGATACTGTCTGGAATTAACCACATTAGGAACTTGTCTAGGAAATGCAAAGCATAGTGAGAATTACGCTGAAGTTGAAGATGCTCCGTTTAGTTTAAACTTAAATTTAGTTAATCCACAGAAATTTGAAACGAATAGATTGTGCATCCCCCTCCTTCTAGTAATATGCAAATTTGGATTCAAGGAAGGTACCACTTTCAAATTAAAGGATATAATTATAGTTTTGTATTCCCTCGTCTATTTTAATTTTTATCTGATCATTATCCTTTCTTTTATTTCCACTGTTCTCTATTAGTCCATCCAACGTCTTCTCCTTCATTTCTTTCAAGTATGTAATTTTGTAGGGTGTTTGTTTGTCATTATGGTTCTTGTTTTTCTTGCTTGCATTTTAACGATGTTCATGGAAACAACTTGAATATATGCAATGGATGCTCCTATTTTCTTGGTAGATAGAAATTAAAATCTAATTTATATTCTCCTTTTCAGCTGGAACTTATAATGACACAAAGTACAAACTAATTGCTGCAATACATGCACTGAACAATGACACAAGTGTGTCGGAGGACATGTACGTGGGCGAATGCCAAGACTCGAGTAACTTTGATCAGAATCTGATCGAAGGGAACCTTCTAATCTGCAGCTATTCAATCAGATTTGTTCTAGGGCTTTCCACGGTTAAACAGGCTTTACAGACAGCAAAAAACTTGAGTGCTACTGGTGTCATTTTCTATATGGATTCTTTTGTGATAGGTTTTAGGCTCAACCCAATTCCTATGAAAATGCCTGGCATCATTATTTCATCACCAGAAGATTCCAAGGTTGCGCTCTACCTTGTTTGTTAGCCTTTAGAATTAACATGCTCTTTAACTTTTCCACTCTTAGGTTTTAGAATTTTATGGATCAATGTAATAAGTTGGGGCATTAAAATGGATTAATTAGCTCCCCCGCACTCTTGCATTCTATGGCTCTGAAATTCATGGTTTCTTTGGCAGATGCTTCTTCAATACTACAATTCTTCCTTGGAAGTAGATGGATTAACAAAGAAAATTTCTAAATTTGGAGCCGTTGCTAGCATATGTGGAGGATTGAAGGCAAATTATAGCTCTTCTGCCCCACAAATTATGTACTACTCTGCTAGAGGACCAGATCCTGAAGACAGTTCTCTTGATGATTCTGATATTATGAAGCCCAACTTGGTAGCTCCTGGAAATTTCATATGGGCTGCTTGGAGTTCTGTTGCCACCGACTCTATCGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAA

mRNA sequence

ATGGATATTATTTACTGTGCACGTCTACTATGTGTTGTTGTCTGTTTTGGGATGTTTGTATGCGCGTCTTGTCTGGATGAATTCGGTGATTCAACAGCTGTTTACATTGTAACTCTCAAGGAACCTCCTTCTGCTACTCATTATTATGGTCAGCTTAGACAAAATACCACTTCTTTTAGTACTTCTGGTGGATTAAGCATCCATAAAGCAAGAAACAAATCAAGAAAGCATCAAAGATATAGATCTTACATAGCTCGAGTTCATGATTCATTGTTGAAGAAGGTCTTGAGGGGAGAGAAATATCTGAAGCTGTACAGTTACCATTTCTTGATCAATGGATTTGCTGTGCTTGTTACCGAAGAACAGGCAAATAAACTTTCAAAGAGGATAGAGGTGGCAAATGTTGTCATGGATTTCTCTGTTAGAACTGCTACGACCCATACTCCACAGTTCTTGGGCCTCCCGCAGGGAGCTTGGTCTCAAGATGGTGGCTTTGAATCTGCTGGAGCTGGGATTGTGATAGGGTTCATTGACACTGGCATTGATCCTTCGCACCCCAGTTTTGCTGATGATTTGACTGATAATCCGTTTCCCGTTCCGGCTCACTTCTCTGGAATTTGTGAGCTTGTGGGAGCGCGCCATTTTGCAGCATCAGCTATAACAAGGGGAATATTTAATGCAACTCAGGATTATGCATCACCATTTGATGGTGATGGACATGGCACGCACACAGCTTCAATTGCTGCTGGAAACCATGGCATTCCAGTCATAGTTGCTGGACATCACTTTGGAAATGCCAGTGGATGTATTGCTGTTTACAAGGCACTGTACAAAAGCTTTGGAGGTTTTGCTGCTGATGTTGTTGCTGCAGTTGATCAGTTTATCAATCACACCAATCGGCGTCCTCCTGGTATTGCAACGTTTTTTAATCCCATTGATATGGCGCTGCTCTCTGCTGTAAAGACTGGTATATTTGTTGTGCAAGCGGCTGGCAACACTGGACCAGCACCAAAGAGCATGTCTTCCTTCAGTCCATGGATCTTTACTGTTGGCGCTGCTTCTCACGATAGAAGCTATGCTAACTCTATAAGCCTTGGCAATAATGTCACCATCCCGGGAGTCGGACTTGCACCTGGAACTTATAATGACACAAAGTACAAACTAATTGCTGCAATACATGCACTGAACAATGACACAAGTGTGTCGGAGGACATGTACGTGGGCGAATGCCAAGACTCGAGTAACTTTGATCAGAATCTGATCGAAGGGAACCTTCTAATCTGCAGCTATTCAATCAGATTTGTTCTAGGGCTTTCCACGGTTAAACAGGCTTTACAGACAGCAAAAAACTTGAGTGCTACTGGTGTCATTTTCTATATGGATTCTTTTGTGATAGGTTTTAGGCTCAACCCAATTCCTATGAAAATGCCTGGCATCATTATTTCATCACCAGAAGATTCCAAGATGCTTCTTCAATACTACAATTCTTCCTTGGAAGTAGATGGATTAACAAAGAAAATTTCTAAATTTGGAGCCGTTGCTAGCATATGTGGAGGATTGAAGGCAAATTATAGCTCTTCTGCCCCACAAATTATGTACTACTCTGCTAGAGGACCAGATCCTGAAGACAGTTCTCTTGATGATTCTGATATTATGAAGCCCAACTTGGTAGCTCCTGGAAATTTCATATGGGCTGCTTGGAGTTCTGTTGCCACCGACTCTATCGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAA

Coding sequence (CDS)

ATGGATATTATTTACTGTGCACGTCTACTATGTGTTGTTGTCTGTTTTGGGATGTTTGTATGCGCGTCTTGTCTGGATGAATTCGGTGATTCAACAGCTGTTTACATTGTAACTCTCAAGGAACCTCCTTCTGCTACTCATTATTATGGTCAGCTTAGACAAAATACCACTTCTTTTAGTACTTCTGGTGGATTAAGCATCCATAAAGCAAGAAACAAATCAAGAAAGCATCAAAGATATAGATCTTACATAGCTCGAGTTCATGATTCATTGTTGAAGAAGGTCTTGAGGGGAGAGAAATATCTGAAGCTGTACAGTTACCATTTCTTGATCAATGGATTTGCTGTGCTTGTTACCGAAGAACAGGCAAATAAACTTTCAAAGAGGATAGAGGTGGCAAATGTTGTCATGGATTTCTCTGTTAGAACTGCTACGACCCATACTCCACAGTTCTTGGGCCTCCCGCAGGGAGCTTGGTCTCAAGATGGTGGCTTTGAATCTGCTGGAGCTGGGATTGTGATAGGGTTCATTGACACTGGCATTGATCCTTCGCACCCCAGTTTTGCTGATGATTTGACTGATAATCCGTTTCCCGTTCCGGCTCACTTCTCTGGAATTTGTGAGCTTGTGGGAGCGCGCCATTTTGCAGCATCAGCTATAACAAGGGGAATATTTAATGCAACTCAGGATTATGCATCACCATTTGATGGTGATGGACATGGCACGCACACAGCTTCAATTGCTGCTGGAAACCATGGCATTCCAGTCATAGTTGCTGGACATCACTTTGGAAATGCCAGTGGATGTATTGCTGTTTACAAGGCACTGTACAAAAGCTTTGGAGGTTTTGCTGCTGATGTTGTTGCTGCAGTTGATCAGTTTATCAATCACACCAATCGGCGTCCTCCTGGTATTGCAACGTTTTTTAATCCCATTGATATGGCGCTGCTCTCTGCTGTAAAGACTGGTATATTTGTTGTGCAAGCGGCTGGCAACACTGGACCAGCACCAAAGAGCATGTCTTCCTTCAGTCCATGGATCTTTACTGTTGGCGCTGCTTCTCACGATAGAAGCTATGCTAACTCTATAAGCCTTGGCAATAATGTCACCATCCCGGGAGTCGGACTTGCACCTGGAACTTATAATGACACAAAGTACAAACTAATTGCTGCAATACATGCACTGAACAATGACACAAGTGTGTCGGAGGACATGTACGTGGGCGAATGCCAAGACTCGAGTAACTTTGATCAGAATCTGATCGAAGGGAACCTTCTAATCTGCAGCTATTCAATCAGATTTGTTCTAGGGCTTTCCACGGTTAAACAGGCTTTACAGACAGCAAAAAACTTGAGTGCTACTGGTGTCATTTTCTATATGGATTCTTTTGTGATAGGTTTTAGGCTCAACCCAATTCCTATGAAAATGCCTGGCATCATTATTTCATCACCAGAAGATTCCAAGATGCTTCTTCAATACTACAATTCTTCCTTGGAAGTAGATGGATTAACAAAGAAAATTTCTAAATTTGGAGCCGTTGCTAGCATATGTGGAGGATTGAAGGCAAATTATAGCTCTTCTGCCCCACAAATTATGTACTACTCTGCTAGAGGACCAGATCCTGAAGACAGTTCTCTTGATGATTCTGATATTATGAAGCCCAACTTGGTAGCTCCTGGAAATTTCATATGGGCTGCTTGGAGTTCTGTTGCCACCGACTCTATCGAATTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTAA

Protein sequence

MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGICELVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGCIAVYKALYKSFGGFAADVVAAVDQFINHTNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE**********************************

Homology

[Close]

BLAST of IVF0013556 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 2.8e-215
Identity = 376/578 (65.05%), Postives = 454/578 (78.55%), Query Frame = 0


Query: 30  DSTAVYIVTLKEPPSATHYYGQ-LRQNTTSFSTSGGLSIHKARNKSRK-HQRYR-SYIAR 89
           D +AVYIVTLK+PP    +  Q L+   + F+        + RN SRK H + +   + +
Sbjct: 34  DDSAVYIVTLKQPPIVHLFEEQELKHKKSKFTPK-----LRPRNNSRKRHGKSKIPSVVQ 93

Query: 90  VHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATT 149
            HDS L+K L+GEKY+KLYSYH+LINGFA+ +  +QA KLS R EVAN+V+D+SVRTATT
Sbjct: 94  SHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATT 153

Query: 150 HTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGI 209
           +TPQF+GLPQGAW ++GGFE AG G++IGFIDTGIDP+HPSF D+ +   +P+P HFSG+
Sbjct: 154 YTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGV 213

Query: 210 CE-------------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHG 269
           CE             L+GARHFA SA+TRGIFN+++DYASPFDGDGHGTHTAS+AAGNHG
Sbjct: 214 CEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHG 273

Query: 270 IPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH----------T 329
           +PVIV+ H+FG ASG      I+VYKALYKSFGGFAADVVAA+DQ               
Sbjct: 274 VPVIVSNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 333

Query: 330 NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRS 389
           NR+PPG+ATFFNPIDMALLSAVK GIFVVQAAGNTGPAPK+MSSFSPWIFTVGA+SHDR 
Sbjct: 334 NRKPPGVATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRV 393

Query: 390 YANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQ 449
           Y+NS++LGNNVTIPG+G A  T +   YK+I+A HALNN TSV +DMYVGECQD  NFDQ
Sbjct: 394 YSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQ 453

Query: 450 NLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPG 509
           + + G LLICSYS RFVLGLST+KQAL  AKNLSATGVIFY+D +V+GF +NP PM MPG
Sbjct: 454 DRVSGKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPG 513

Query: 510 IIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARG 569
           III S EDSK LL+YYNSS++ D  TK+I  FGAVA+I GGL AN+S+ AP++MYYSARG
Sbjct: 514 IIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARG 573

Query: 570 PDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 577
           PDPED+S +D+D++KPNLVAPGN IW AWSS +TDS E
Sbjct: 574 PDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTE 606

[Close]

BLAST of IVF0013556 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 4.9e-212
Identity = 387/611 (63.34%), Postives = 466/611 (76.27%), Query Frame = 0


Query: 12  VVVCFGMFVCASCLDEFGDS-----TAVYIVTLKEPPSATHYY-----------GQLRQN 71
           ++ CFG+   +   D  GDS     TAVYIVTL++  S+ H +            Q +  
Sbjct: 13  LLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQ-ASSLHLFQQEAEEVKRVRDQSKHG 72

Query: 72  TTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFA 131
            TS  T   L      ++SR  +  RS IA+ HDSLL+  L+GEKY+KLYS+H+LINGFA
Sbjct: 73  DTSKFTRPKLQPRNI-SRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGFA 132

Query: 132 VLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIG 191
           V V+ +QA  LS+R EVAN+V+DFSVRTATT+TPQF+GLP+GAW ++GG+E+AG GIVIG
Sbjct: 133 VFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVIG 192

Query: 192 FIDTGIDPSHPSF-ADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAIT 251
           FIDTGIDP+HPSF   D +   +P+P HFSG+CE             LVGARHFA SAIT
Sbjct: 193 FIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAIT 252

Query: 252 RGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKAL 311
           RGIFN+++DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG      I+VYKAL
Sbjct: 253 RGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKAL 312

Query: 312 YKSFGGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFV 371
           YKSFGGFAADVVAA+DQ               NRRPPG+ATFFNP+DMA+LSAVK GIFV
Sbjct: 313 YKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIFV 372

Query: 372 VQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKY 431
           VQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T    KY
Sbjct: 373 VQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKKY 432

Query: 432 KLIAAIHALNNDTS-VSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQAL 491
            +I+A+ AL N +S V +DMYVGECQD  +FD+++I GNLLICSYSIRFVLGLST+KQAL
Sbjct: 433 TMISALDALKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQAL 492

Query: 492 QTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTK 551
             AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG TK
Sbjct: 493 AVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTK 552

Query: 552 KISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWA 577
           +I +FGAVA+I GG  AN+S+ AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGN IW 
Sbjct: 553 EIVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWG 612

[Close]

BLAST of IVF0013556 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 1.3e-196
Identity = 357/588 (60.71%), Postives = 442/588 (75.17%), Query Frame = 0


Query: 9   LLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIH 68
           LLC +V   +F  A        S+AVYIVTLK+ PS  H+ G+   ++    T+    I+
Sbjct: 12  LLC-LVSSSVFCLAESDQNATVSSAVYIVTLKDRPS-VHFSGRESSDSKHSLTATSSQIY 71

Query: 69  KARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSK 128
           +  N+S       + I RVHDSLL+ VLR E YLKLYSYH+LINGF+ ++T +QA++L+ 
Sbjct: 72  RTLNRS-------ASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLAA 131

Query: 129 RIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSF 188
           R EV NVV+DF V  ATTHTPQFLGLP+GAW +DGG E AG G+VIGFIDTGIDP+HPSF
Sbjct: 132 REEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSF 191

Query: 189 ADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNATQDYASPF 248
           +D ++ + + VP HF+G+CE             L+GARHFA SA++RG+ N++QD ASPF
Sbjct: 192 SDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPF 251

Query: 249 DGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAA 308
           DG+GHGTHTAS+AAGNHGIPV+VAGH  GNASG      IA+YKALYK FGGFAAD++AA
Sbjct: 252 DGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAA 311

Query: 309 VDQ-------FINHT---NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSM 368
           +DQ        IN +   NRRPPGIATFFNPIDMALLSAVK GIFVVQAAGNTGPAPKSM
Sbjct: 312 IDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSM 371

Query: 369 SSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTS 428
           SSFSPWIFTVGA SHDR Y+NSI LGNNVTIPGVGLA GT     +KL+ A HAL N T+
Sbjct: 372 SSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT--RIMHKLVLATHALRNGTT 431

Query: 429 VSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYM 488
           V + +YVGECQDSS+FDQ L++G +L+CSY++RF+LG+ST+KQAL TAKNL+A G++FY+
Sbjct: 432 VMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYI 491

Query: 489 DSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGL 548
           D    GF++   PM +PGI+ISSP+DS+ LL+YYNSSL  +  + KI    +VA I GG+
Sbjct: 492 DPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGM 551

Query: 549 KANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWS 569
           +  Y  +AP++MY+SARGPDPED S  D+DIMKPNLVAPGN IW AWS
Sbjct: 552 RPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGNAIWGAWS 588

[Close]

BLAST of IVF0013556 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.4e-121
Identity = 252/569 (44.29%), Postives = 335/569 (58.88%), Query Frame = 0


Query: 34  VYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLK 93
           VYIVT++  P  ++  G+       F  +   S  K    S     Y  ++ R HD +L 
Sbjct: 21  VYIVTMEGDPIISYKGGE-----NGFEATAVESDEKIDTSSELVTVYARHLERKHDMILG 80

Query: 94  KVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLG 153
            +     Y KLYSY  LINGFA  V+ EQA  L +   V +V  D+ VR  TTHTP+FLG
Sbjct: 81  MLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLG 140

Query: 154 LPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGICE----- 213
           LP   W   GGF+ AG  IVIGF+D+GI P HPSFA        P+P H+ G CE     
Sbjct: 141 LPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLP-HYKGKCEEDPHT 200

Query: 214 --------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAG 273
                   +VGA+HFA +A   G FN   DYASP DGDGHG+HTA+IAAGN+GIP+ + G
Sbjct: 201 KKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHG 260

Query: 274 HHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH---------TNRRPPGI- 333
           + FG ASG      IAVYKALY+ FGGF ADVVAA+DQ ++              PP   
Sbjct: 261 YEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTT 320

Query: 334 -ATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSIS 393
             TF NP D  LL AVK G+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N ++
Sbjct: 321 KTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLT 380

Query: 394 LGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGN 453
           LGN   + G+GL+P T     Y L++A   L  D+SVS+     +CQ    F++ L+EGN
Sbjct: 381 LGNGKMLAGMGLSPPTRPHRLYTLVSANDVL-LDSSVSK-YNPSDCQRPEVFNKKLVEGN 440

Query: 454 LLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSP 513
           +L+C YS  FV+G +++K+ + TAK+L A G +  +++   G + +P+P  +PGI+I+  
Sbjct: 441 ILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDV 500

Query: 514 EDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDS 573
             S  L+ YYN+S   D  T ++  F A  SI  GL      SAPQ+  +SARGP+ +D 
Sbjct: 501 SKSMDLIDYYNASTSRD-WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDF 560

[Close]

BLAST of IVF0013556 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.0e-120
Identity = 242/569 (42.53%), Postives = 332/569 (58.35%), Query Frame = 0


Query: 34  VYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLK 93
           +YIVT++  P  ++  G        F  +   S  K    S     Y  ++ R HD LL 
Sbjct: 21  IYIVTMEGEPIISYKGGD-----NGFEATAVESDEKIDTTSELVTSYARHLERKHDMLLG 80

Query: 94  KVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLG 153
            +     Y KLYSY  LINGFA  V+ +QA  L +   V +V  D+ VR  TTHTPQFLG
Sbjct: 81  MLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKLTTHTPQFLG 140

Query: 154 LPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGICE----- 213
           LP   W   GG++ AG  IVIGFID+GI P HPSFA   T  P+     + G CE     
Sbjct: 141 LPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYKGKCEEDPHT 200

Query: 214 --------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAG 273
                   ++GA+HFA +A   G FN   D+ASP DGDGHG+HTA+IAAGN+GIPV + G
Sbjct: 201 KISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGNNGIPVRMHG 260

Query: 274 HHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH---------TNRRPPGI- 333
           + FG ASG      IAVYKALY+ FGGF ADVVAA+DQ ++              PP   
Sbjct: 261 YEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATT 320

Query: 334 -ATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSIS 393
             TF NP D  LL AVK G+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N ++
Sbjct: 321 KTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLT 380

Query: 394 LGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGN 453
           LGN   + G+GL+P T     YK+++A   L   + +  +    +CQ     ++ L+EGN
Sbjct: 381 LGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYN--PSDCQKPEVLNKKLVEGN 440

Query: 454 LLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSP 513
           +L+C YS  FV G +++K+  +TAK+L A G +  +++   G + +P+P  +PGI+I+  
Sbjct: 441 ILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIPGILITDV 500

Query: 514 EDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDS 573
             S  L+ YYN +   D +  ++  F A  SI  GL+     SAP++  +SARGP+ +D 
Sbjct: 501 SKSMDLIDYYNVTTSRDWM-GRVKDFKAEGSIGDGLEPILHKSAPEVALFSARGPNTKDF 560

[Close]

BLAST of IVF0013556 vs. ExPASy TrEMBL
Match: A0A1S3AZJ6 (subtilisin-like protease SBT2.3 OS=Cucumis melo OX=3656 GN=LOC103484283 PE=3 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 569/604 (94.21%), Postives = 569/604 (94.21%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60
           MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS
Sbjct: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60

Query: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120
           TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE
Sbjct: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120

Query: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180
           EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG
Sbjct: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180

Query: 181 IDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNA 240
           IDPSHPSFADDLTDNPFPVPAHFSGICE             LVGARHFAASAITRGIFNA
Sbjct: 181 IDPSHPSFADDLTDNPFPVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 240

Query: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGG 300
           TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGG
Sbjct: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGG 300

Query: 301 FAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360
           FAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN
Sbjct: 301 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360

Query: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420
           TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI
Sbjct: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420

Query: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480
           HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS
Sbjct: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480

Query: 481 ATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540
           A GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA
Sbjct: 481 AAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540

Query: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 577
           VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT
Sbjct: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 600

[Close]

BLAST of IVF0013556 vs. ExPASy TrEMBL
Match: A0A0A0KMB5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G526310 PE=3 SV=1)

HSP 1 Score: 1097.4 bits (2837), Expect = 0.0e+00
Identity = 559/604 (92.55%), Postives = 565/604 (93.54%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60
           MDII+C RLLCVVVCFG+FVCASCLDEFGDSTAVYIVTLKEPPS THYYGQLRQNTTSFS
Sbjct: 1   MDIIHCTRLLCVVVCFGVFVCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSFS 60

Query: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120
           TSGGLSIHKARN SRKH+RYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE
Sbjct: 61  TSGGLSIHKARNISRKHRRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120

Query: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180
           EQANKLSKR EVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG
Sbjct: 121 EQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180

Query: 181 IDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNA 240
           IDPSHPSFADDLTDNPFP+PAHFSGICE             LVGARHFAASAITRGIFNA
Sbjct: 181 IDPSHPSFADDLTDNPFPIPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 240

Query: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGG 300
           TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGG
Sbjct: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGG 300

Query: 301 FAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360
           FAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN
Sbjct: 301 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360

Query: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420
           TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNN+TIPGVGLAPGTYNDTKYKLIAAI
Sbjct: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNITIPGVGLAPGTYNDTKYKLIAAI 420

Query: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480
           HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS
Sbjct: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480

Query: 481 ATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540
           A GVIFYMDSFVIGFRLNPIPMKMPGII+SSPEDSKMLLQYYNSSLEVDGLTKKISKFGA
Sbjct: 481 AAGVIFYMDSFVIGFRLNPIPMKMPGIIVSSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540

Query: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 577
           VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT
Sbjct: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 600

[Close]

BLAST of IVF0013556 vs. ExPASy TrEMBL
Match: A0A5A7U976 (Subtilisin-like protease SBT2.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009960 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 553/587 (94.21%), Postives = 553/587 (94.21%), Query Frame = 0


Query: 18  MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH 77
           MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH
Sbjct: 1   MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH 60

Query: 78  QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM 137
           QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM
Sbjct: 61  QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM 120

Query: 138 DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF 197
           DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF
Sbjct: 121 DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF 180

Query: 198 PVPAHFSGICE-------------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT 257
           PVPAHFSGICE             LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT
Sbjct: 181 PVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT 240

Query: 258 ASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH-- 317
           ASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGGFAADVVAAVDQ      
Sbjct: 241 ASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVAAVDQAAQDGV 300

Query: 318 --------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT 377
                    NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT
Sbjct: 301 DIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT 360

Query: 378 VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE 437
           VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE
Sbjct: 361 VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE 420

Query: 438 CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL 497
           CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL
Sbjct: 421 CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL 480

Query: 498 NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP 557
           NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP
Sbjct: 481 NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP 540

Query: 558 QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 577
           QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE
Sbjct: 541 QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 587

[Close]

BLAST of IVF0013556 vs. ExPASy TrEMBL
Match: A0A6J1IJ51 (subtilisin-like protease SBT2.3 OS=Cucurbita maxima OX=3661 GN=LOC111477908 PE=3 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.3e-303
Identity = 541/606 (89.27%), Postives = 558/606 (92.08%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSF- 60
           MDII+ ARLLC VVCFGMF+CASCLDEFGDSTAVYIVTLKEPP+ THYYGQLRQNTTSF 
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPT-THYYGQLRQNTTSFR 60

Query: 61  -STSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120
              SGGLSIHK RN SRKH+RYRSYIARVHDSLLKKVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNVSRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180
           TE+QANKLSKR+EVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIV+GF+D
Sbjct: 121 TEQQANKLSKRMEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVMGFVD 180

Query: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIF 240
           TGIDPSHPSFADDL+D PFPVP HFSGICE             LVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASG      IAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQ-------FINHT---NRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360
           GGFAADVVAAVDQ        IN +   NRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN TKYKLIA
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKYKLIA 420

Query: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480
           AIHALNN T+VS DMYVGECQDSSNFDQ+LI+GNLLICSYSIRFVLGLSTVKQALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNFDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540
           LSA GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 577
           GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

[Close]

BLAST of IVF0013556 vs. ExPASy TrEMBL
Match: A0A6J1EDC1 (subtilisin-like protease SBT2.2 OS=Cucurbita moschata OX=3662 GN=LOC111433134 PE=3 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.8e-303
Identity = 539/606 (88.94%), Postives = 552/606 (91.09%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSF- 60
           MDII+ ARLLC VVCFGMF+CASCLDEFGDSTAVYIVTLKEPP  THYYGQLRQNTTSF 
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPPTTHYYGQLRQNTTSFR 60

Query: 61  -STSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120
              SGGLSIHK RN SRKH+RYRSYIARVHDSLLKKVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180
           TEEQANKLSKR EVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGF+D
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFVD 180

Query: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIF 240
           TGIDPSHPSFADDL+D PFPVP HFSGICE             LVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASG      IAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360
           GGFAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN TK KLIA
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKNKLIA 420

Query: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480
           AIHALNN T+VS DMYVGECQDSSN+DQ+LI+GNLLICSYSIRFVLGLSTVKQALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNYDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540
           LSA GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 577
           GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

[Close]

BLAST of IVF0013556 vs. NCBI nr
Match: XP_008439503.1 (PREDICTED: subtilisin-like protease SBT2.3 [Cucumis melo])

HSP 1 Score: 1114 bits (2881), Expect = 0.0
Identity = 569/604 (94.21%), Postives = 569/604 (94.21%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60
           MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS
Sbjct: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60

Query: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120
           TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE
Sbjct: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120

Query: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180
           EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG
Sbjct: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180

Query: 181 IDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNA 240
           IDPSHPSFADDLTDNPFPVPAHFSGICE             LVGARHFAASAITRGIFNA
Sbjct: 181 IDPSHPSFADDLTDNPFPVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 240

Query: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGG 300
           TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGG
Sbjct: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGG 300

Query: 301 FAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360
           FAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN
Sbjct: 301 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360

Query: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420
           TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI
Sbjct: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420

Query: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480
           HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS
Sbjct: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480

Query: 481 ATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540
           A GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA
Sbjct: 481 AAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540

Query: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 576
           VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT
Sbjct: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 600

[Close]

BLAST of IVF0013556 vs. NCBI nr
Match: XP_011658335.1 (subtilisin-like protease SBT2.3 [Cucumis sativus] >KGN49497.1 hypothetical protein Csa_004413 [Cucumis sativus])

HSP 1 Score: 1099 bits (2842), Expect = 0.0
Identity = 559/604 (92.55%), Postives = 565/604 (93.54%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFS 60
           MDII+C RLLCVVVCFG+FVCASCLDEFGDSTAVYIVTLKEPPS THYYGQLRQNTTSFS
Sbjct: 1   MDIIHCTRLLCVVVCFGVFVCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSFS 60

Query: 61  TSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120
           TSGGLSIHKARN SRKH+RYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE
Sbjct: 61  TSGGLSIHKARNISRKHRRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTE 120

Query: 121 EQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180
           EQANKLSKR EVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG
Sbjct: 121 EQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTG 180

Query: 181 IDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNA 240
           IDPSHPSFADDLTDNPFP+PAHFSGICE             LVGARHFAASAITRGIFNA
Sbjct: 181 IDPSHPSFADDLTDNPFPIPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNA 240

Query: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGG 300
           TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGG
Sbjct: 241 TQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGG 300

Query: 301 FAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360
           FAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN
Sbjct: 301 FAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGN 360

Query: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAI 420
           TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNN+TIPGVGLAPGTYNDTKYKLIAAI
Sbjct: 361 TGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNITIPGVGLAPGTYNDTKYKLIAAI 420

Query: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480
           HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS
Sbjct: 421 HALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLS 480

Query: 481 ATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540
           A GVIFYMDSFVIGFRLNPIPMKMPGII+SSPEDSKMLLQYYNSSLEVDGLTKKISKFGA
Sbjct: 481 AAGVIFYMDSFVIGFRLNPIPMKMPGIIVSSPEDSKMLLQYYNSSLEVDGLTKKISKFGA 540

Query: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 576
           VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT
Sbjct: 541 VASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVAT 600

[Close]

BLAST of IVF0013556 vs. NCBI nr
Match: XP_038883866.1 (subtilisin-like protease SBT2.2 [Benincasa hispida])

HSP 1 Score: 1085 bits (2807), Expect = 0.0
Identity = 553/606 (91.25%), Postives = 564/606 (93.07%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSF- 60
           MDII+CARLLCVVVCFGMF+CASCLDEFGDSTAVYIVTLKEPPS THYYGQLRQNTTSF 
Sbjct: 1   MDIIHCARLLCVVVCFGMFLCASCLDEFGDSTAVYIVTLKEPPSTTHYYGQLRQNTTSFR 60

Query: 61  -STSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120
             TSGGLSIHK RN SRKH+RYRSYIARVHDSLLKKVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGTSGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180
           TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID
Sbjct: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180

Query: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIF 240
           TGIDPSHPSFADDLTDNPFPVPAHFSGICE             LVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360
           GGFAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420

Query: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480
           AIHALNNDT+VSEDMYVGECQDSSNFDQ+L++GNLLICSYSIRFVLGLSTVKQALQTAKN
Sbjct: 421 AIHALNNDTNVSEDMYVGECQDSSNFDQDLVQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540
           LSA GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540

Query: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 576
           GAVASICGGLKANYSS+APQ+MYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSTAPQVMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 600

[Close]

BLAST of IVF0013556 vs. NCBI nr
Match: KAA0052453.1 (subtilisin-like protease SBT2.3 [Cucumis melo var. makuwa] >TYK13369.1 subtilisin-like protease SBT2.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 553/587 (94.21%), Postives = 553/587 (94.21%), Query Frame = 0


Query: 18  MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH 77
           MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH
Sbjct: 1   MFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKH 60

Query: 78  QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM 137
           QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM
Sbjct: 61  QRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVM 120

Query: 138 DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF 197
           DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF
Sbjct: 121 DFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPF 180

Query: 198 PVPAHFSGICE-------------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT 257
           PVPAHFSGICE             LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT
Sbjct: 181 PVPAHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIFNATQDYASPFDGDGHGTHT 240

Query: 258 ASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH-- 317
           ASIAAGNHGIPVIVAGHHFGNASG      IAVYKALYKSFGGFAADVVAAVDQ      
Sbjct: 241 ASIAAGNHGIPVIVAGHHFGNASGMAPRSHIAVYKALYKSFGGFAADVVAAVDQAAQDGV 300

Query: 318 --------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT 377
                    NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT
Sbjct: 301 DIISLSITPNRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFT 360

Query: 378 VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE 437
           VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE
Sbjct: 361 VGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGE 420

Query: 438 CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL 497
           CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL
Sbjct: 421 CQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRL 480

Query: 498 NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP 557
           NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP
Sbjct: 481 NPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAP 540

Query: 558 QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 576
           QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE
Sbjct: 541 QIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 587

[Close]

BLAST of IVF0013556 vs. NCBI nr
Match: XP_023543118.1 (subtilisin-like protease SBT2.2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1055 bits (2729), Expect = 0.0
Identity = 540/606 (89.11%), Postives = 553/606 (91.25%), Query Frame = 0


Query: 1   MDIIYCARLLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSF- 60
           MDII+ ARLLC VVCFGMF+CASCLDEFGDSTAVYIVTLKEPP  THYYGQLRQNTTSF 
Sbjct: 1   MDIIHRARLLCAVVCFGMFICASCLDEFGDSTAVYIVTLKEPPPTTHYYGQLRQNTTSFR 60

Query: 61  -STSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLV 120
              SGGLSIHK RN SRKH+RYRSYIARVHDSLLKKVL+GEKYLKLYSYHFLINGFAVLV
Sbjct: 61  LGASGGLSIHKPRNISRKHRRYRSYIARVHDSLLKKVLKGEKYLKLYSYHFLINGFAVLV 120

Query: 121 TEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFID 180
           TEEQANKLSKR EVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGF+D
Sbjct: 121 TEEQANKLSKRKEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFVD 180

Query: 181 TGIDPSHPSFADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIF 240
           TGIDPSHPSFADDL+D PFPVP HFSGICE             LVGARHFAASAITRGIF
Sbjct: 181 TGIDPSHPSFADDLSDKPFPVPPHFSGICEVTPDFPSGSCNRKLVGARHFAASAITRGIF 240

Query: 241 NATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSF 300
           NA+QDYASPFDGDGHGTHTASIAAGNHGIPV+VAGHHFGNASG      IAVYKALYKSF
Sbjct: 241 NASQDYASPFDGDGHGTHTASIAAGNHGIPVLVAGHHFGNASGMAPRSHIAVYKALYKSF 300

Query: 301 GGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFVVQAA 360
           GGFAADVVAAVDQ               NRRPPGIATFFNPIDMALLSAVK GIFVVQAA
Sbjct: 301 GGFAADVVAAVDQAAQDGVDIISLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAA 360

Query: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIA 420
           GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYN TKYKLIA
Sbjct: 361 GNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNGTKYKLIA 420

Query: 421 AIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKN 480
           AIHALNN T+VS DMYVGECQDSSN+DQ+LI+GNLLICSYSIRFVLGLSTVKQALQTAKN
Sbjct: 421 AIHALNNGTNVSADMYVGECQDSSNYDQDLIQGNLLICSYSIRFVLGLSTVKQALQTAKN 480

Query: 481 LSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKF 540
           LSA GVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTK+ISKF
Sbjct: 481 LSAAGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKRISKF 540

Query: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSV 576
           GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGN IWAAWSSV
Sbjct: 541 GAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNSIWAAWSSV 600

[Close]

BLAST of IVF0013556 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 749.6 bits (1934), Expect = 2.0e-216
Identity = 376/578 (65.05%), Postives = 454/578 (78.55%), Query Frame = 0


Query: 30  DSTAVYIVTLKEPPSATHYYGQ-LRQNTTSFSTSGGLSIHKARNKSRK-HQRYR-SYIAR 89
           D +AVYIVTLK+PP    +  Q L+   + F+        + RN SRK H + +   + +
Sbjct: 34  DDSAVYIVTLKQPPIVHLFEEQELKHKKSKFTPK-----LRPRNNSRKRHGKSKIPSVVQ 93

Query: 90  VHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATT 149
            HDS L+K L+GEKY+KLYSYH+LINGFA+ +  +QA KLS R EVAN+V+D+SVRTATT
Sbjct: 94  SHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATT 153

Query: 150 HTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGI 209
           +TPQF+GLPQGAW ++GGFE AG G++IGFIDTGIDP+HPSF D+ +   +P+P HFSG+
Sbjct: 154 YTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGV 213

Query: 210 CE-------------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHG 269
           CE             L+GARHFA SA+TRGIFN+++DYASPFDGDGHGTHTAS+AAGNHG
Sbjct: 214 CEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHG 273

Query: 270 IPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH----------T 329
           +PVIV+ H+FG ASG      I+VYKALYKSFGGFAADVVAA+DQ               
Sbjct: 274 VPVIVSNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 333

Query: 330 NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRS 389
           NR+PPG+ATFFNPIDMALLSAVK GIFVVQAAGNTGPAPK+MSSFSPWIFTVGA+SHDR 
Sbjct: 334 NRKPPGVATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDRV 393

Query: 390 YANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQ 449
           Y+NS++LGNNVTIPG+G A  T +   YK+I+A HALNN TSV +DMYVGECQD  NFDQ
Sbjct: 394 YSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFDQ 453

Query: 450 NLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPG 509
           + + G LLICSYS RFVLGLST+KQAL  AKNLSATGVIFY+D +V+GF +NP PM MPG
Sbjct: 454 DRVSGKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMPG 513

Query: 510 IIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARG 569
           III S EDSK LL+YYNSS++ D  TK+I  FGAVA+I GGL AN+S+ AP++MYYSARG
Sbjct: 514 IIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSARG 573

Query: 570 PDPEDSSLDDSDIMKPNLVAPGNFIWAAWSSVATDSIE 577
           PDPED+S +D+D++KPNLVAPGN IW AWSS +TDS E
Sbjct: 574 PDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTE 606

[Close]

BLAST of IVF0013556 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 738.8 bits (1906), Expect = 3.5e-213
Identity = 387/611 (63.34%), Postives = 466/611 (76.27%), Query Frame = 0


Query: 12  VVVCFGMFVCASCLDEFGDS-----TAVYIVTLKEPPSATHYY-----------GQLRQN 71
           ++ CFG+   +   D  GDS     TAVYIVTL++  S+ H +            Q +  
Sbjct: 13  LLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQ-ASSLHLFQQEAEEVKRVRDQSKHG 72

Query: 72  TTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFA 131
            TS  T   L      ++SR  +  RS IA+ HDSLL+  L+GEKY+KLYS+H+LINGFA
Sbjct: 73  DTSKFTRPKLQPRNI-SRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGFA 132

Query: 132 VLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIG 191
           V V+ +QA  LS+R EVAN+V+DFSVRTATT+TPQF+GLP+GAW ++GG+E+AG GIVIG
Sbjct: 133 VFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVIG 192

Query: 192 FIDTGIDPSHPSF-ADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAIT 251
           FIDTGIDP+HPSF   D +   +P+P HFSG+CE             LVGARHFA SAIT
Sbjct: 193 FIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAIT 252

Query: 252 RGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKAL 311
           RGIFN+++DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG      I+VYKAL
Sbjct: 253 RGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKAL 312

Query: 312 YKSFGGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFV 371
           YKSFGGFAADVVAA+DQ               NRRPPG+ATFFNP+DMA+LSAVK GIFV
Sbjct: 313 YKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIFV 372

Query: 372 VQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKY 431
           VQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T    KY
Sbjct: 373 VQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKKY 432

Query: 432 KLIAAIHALNNDTS-VSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQAL 491
            +I+A+ AL N +S V +DMYVGECQD  +FD+++I GNLLICSYSIRFVLGLST+KQAL
Sbjct: 433 TMISALDALKNKSSVVDKDMYVGECQDYGSFDKDVIRGNLLICSYSIRFVLGLSTIKQAL 492

Query: 492 QTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTK 551
             AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG TK
Sbjct: 493 AVAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTK 552

Query: 552 KISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWA 577
           +I +FGAVA+I GG  AN+S+ AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGN IW 
Sbjct: 553 EIVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWG 612

[Close]

BLAST of IVF0013556 vs. TAIR 10
Match: AT4G20430.2 (Subtilase family protein )

HSP 1 Score: 688.3 bits (1775), Expect = 5.4e-198
Identity = 369/610 (60.49%), Postives = 446/610 (73.11%), Query Frame = 0


Query: 12  VVVCFGMFVCASCLDEFGDS-----TAVYIVTLKEPPSATHYY-----------GQLRQN 71
           ++ CFG+   +   D  GDS     TAVYIVTL++  S+ H +            Q +  
Sbjct: 13  LLFCFGVLSNSFGQDNGGDSDINSTTAVYIVTLRQ-ASSLHLFQQEAEEVKRVRDQSKHG 72

Query: 72  TTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFA 131
            TS  T   L      ++SR  +  RS IA+ HDSLL+  L+GEKY+KLYS+H+LINGFA
Sbjct: 73  DTSKFTRPKLQPRNI-SRSRYWRSRRSAIAQAHDSLLRNALKGEKYIKLYSFHYLINGFA 132

Query: 132 VLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIG 191
           V V+ +QA  LS+R EVAN+V+DFSVRTATT+TPQF+GLP+GAW ++GG+E+AG GIVIG
Sbjct: 133 VFVSSQQAETLSRRREVANIVLDFSVRTATTYTPQFMGLPKGAWVKEGGYETAGEGIVIG 192

Query: 192 FIDTGIDPSHPSF-ADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAIT 251
           FIDTGIDP+HPSF   D +   +P+P HFSG+CE             LVGARHFA SAIT
Sbjct: 193 FIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGVCEVTPDFPSGSCNRKLVGARHFAQSAIT 252

Query: 252 RGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKAL 311
           RGIFN+++DYASPFDGDGHGTHTASIAAGNHG+  +V+GH+FG+ASG      I+VYKAL
Sbjct: 253 RGIFNSSEDYASPFDGDGHGTHTASIAAGNHGVSAVVSGHNFGSASGIAPRAHISVYKAL 312

Query: 312 YKSFGGFAADVVAAVDQFINH----------TNRRPPGIATFFNPIDMALLSAVKTGIFV 371
           YKSFGGFAADVVAA+DQ               NRRPPG+ATFFNP+DMA+LSAVK GIFV
Sbjct: 313 YKSFGGFAADVVAAIDQAAQDGVDILSLSITPNRRPPGVATFFNPLDMAMLSAVKAGIFV 372

Query: 372 VQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKY 431
           VQAAGNTGP+PKSMSSFSPWIFTVGAASHDR Y+NSI LGNNV+IPGVGLA  T    KY
Sbjct: 373 VQAAGNTGPSPKSMSSFSPWIFTVGAASHDRDYSNSIVLGNNVSIPGVGLALRTDEGKKY 432

Query: 432 KLIAAIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQ 491
            +I+A+ AL N +SV               D+++         YSIRFVLGLST+KQAL 
Sbjct: 433 TMISALDALKNKSSV--------------VDKDI---------YSIRFVLGLSTIKQALA 492

Query: 492 TAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKK 551
            AKNLSA GV+FYMD +V+GF++NP PM MPGIII S EDSK+LL+YYNSSL  DG TK+
Sbjct: 493 VAKNLSAKGVVFYMDPYVLGFQINPTPMDMPGIIIPSAEDSKVLLKYYNSSLVRDGTTKE 552

Query: 552 ISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAA 577
           I +FGAVA+I GG  AN+S+ AP+IMYYSARGPDP+DS  +D+DI+KPNLVAPGN IW A
Sbjct: 553 IVRFGAVAAIAGGQNANFSNRAPKIMYYSARGPDPQDSLFNDADILKPNLVAPGNSIWGA 597

[Close]

BLAST of IVF0013556 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 687.6 bits (1773), Expect = 9.3e-198
Identity = 357/588 (60.71%), Postives = 442/588 (75.17%), Query Frame = 0


Query: 9   LLCVVVCFGMFVCASCLDEFGDSTAVYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIH 68
           LLC +V   +F  A        S+AVYIVTLK+ PS  H+ G+   ++    T+    I+
Sbjct: 12  LLC-LVSSSVFCLAESDQNATVSSAVYIVTLKDRPS-VHFSGRESSDSKHSLTATSSQIY 71

Query: 69  KARNKSRKHQRYRSYIARVHDSLLKKVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSK 128
           +  N+S       + I RVHDSLL+ VLR E YLKLYSYH+LINGF+ ++T +QA++L+ 
Sbjct: 72  RTLNRS-------ASIIRVHDSLLRNVLRKENYLKLYSYHYLINGFSAVLTRKQADRLAA 131

Query: 129 RIEVANVVMDFSVRTATTHTPQFLGLPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSF 188
           R EV NVV+DF V  ATTHTPQFLGLP+GAW +DGG E AG G+VIGFIDTGIDP+HPSF
Sbjct: 132 REEVENVVLDFLVEKATTHTPQFLGLPRGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSF 191

Query: 189 ADDLTDNPFPVPAHFSGICE-------------LVGARHFAASAITRGIFNATQDYASPF 248
           +D ++ + + VP HF+G+CE             L+GARHFA SA++RG+ N++QD ASPF
Sbjct: 192 SDKISGHTYSVPPHFTGVCEVTIGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPF 251

Query: 249 DGDGHGTHTASIAAGNHGIPVIVAGHHFGNASGC-----IAVYKALYKSFGGFAADVVAA 308
           DG+GHGTHTAS+AAGNHGIPV+VAGH  GNASG      IA+YKALYK FGGFAAD++AA
Sbjct: 252 DGEGHGTHTASVAAGNHGIPVVVAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAA 311

Query: 309 VDQ-------FINHT---NRRPPGIATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSM 368
           +DQ        IN +   NRRPPGIATFFNPIDMALLSAVK GIFVVQAAGNTGPAPKSM
Sbjct: 312 IDQAAQDGVDIINLSITPNRRPPGIATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSM 371

Query: 369 SSFSPWIFTVGAASHDRSYANSISLGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTS 428
           SSFSPWIFTVGA SHDR Y+NSI LGNNVTIPGVGLA GT     +KL+ A HAL N T+
Sbjct: 372 SSFSPWIFTVGATSHDRVYSNSIILGNNVTIPGVGLASGT--RIMHKLVLATHALRNGTT 431

Query: 429 VSEDMYVGECQDSSNFDQNLIEGNLLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYM 488
           V + +YVGECQDSS+FDQ L++G +L+CSY++RF+LG+ST+KQAL TAKNL+A G++FY+
Sbjct: 432 VMDAIYVGECQDSSSFDQKLVQGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYI 491

Query: 489 DSFVIGFRLNPIPMKMPGIIISSPEDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGL 548
           D    GF++   PM +PGI+ISSP+DS+ LL+YYNSSL  +  + KI    +VA I GG+
Sbjct: 492 DPSATGFQMTSSPMDIPGILISSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGM 551

Query: 549 KANYSSSAPQIMYYSARGPDPEDSSLDDSDIMKPNLVAPGNFIWAAWS 569
           +  Y  +AP++MY+SARGPDPED S  D+DIMKPNLVAPGN IW AWS
Sbjct: 552 RPTYGITAPKVMYFSARGPDPEDDSFVDADIMKPNLVAPGNAIWGAWS 588

[Close]

BLAST of IVF0013556 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 438.3 bits (1126), Expect = 9.8e-123
Identity = 252/569 (44.29%), Postives = 335/569 (58.88%), Query Frame = 0


Query: 34  VYIVTLKEPPSATHYYGQLRQNTTSFSTSGGLSIHKARNKSRKHQRYRSYIARVHDSLLK 93
           VYIVT++  P  ++  G+       F  +   S  K    S     Y  ++ R HD +L 
Sbjct: 21  VYIVTMEGDPIISYKGGE-----NGFEATAVESDEKIDTSSELVTVYARHLERKHDMILG 80

Query: 94  KVLRGEKYLKLYSYHFLINGFAVLVTEEQANKLSKRIEVANVVMDFSVRTATTHTPQFLG 153
            +     Y KLYSY  LINGFA  V+ EQA  L +   V +V  D+ VR  TTHTP+FLG
Sbjct: 81  MLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRLTTHTPEFLG 140

Query: 154 LPQGAWSQDGGFESAGAGIVIGFIDTGIDPSHPSFADDLTDNPFPVPAHFSGICE----- 213
           LP   W   GGF+ AG  IVIGF+D+GI P HPSFA        P+P H+ G CE     
Sbjct: 141 LPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLP-HYKGKCEEDPHT 200

Query: 214 --------LVGARHFAASAITRGIFNATQDYASPFDGDGHGTHTASIAAGNHGIPVIVAG 273
                   +VGA+HFA +A   G FN   DYASP DGDGHG+HTA+IAAGN+GIP+ + G
Sbjct: 201 KKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNNGIPLRMHG 260

Query: 274 HHFGNASGC-----IAVYKALYKSFGGFAADVVAAVDQFINH---------TNRRPPGI- 333
           + FG ASG      IAVYKALY+ FGGF ADVVAA+DQ ++              PP   
Sbjct: 261 YEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPTTT 320

Query: 334 -ATFFNPIDMALLSAVKTGIFVVQAAGNTGPAPKSMSSFSPWIFTVGAASHDRSYANSIS 393
             TF NP D  LL AVK G+FV QAAGN GP PK++ S+SPWI TV AA  DR Y N ++
Sbjct: 321 KTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDDRRYKNHLT 380

Query: 394 LGNNVTIPGVGLAPGTYNDTKYKLIAAIHALNNDTSVSEDMYVGECQDSSNFDQNLIEGN 453
           LGN   + G+GL+P T     Y L++A   L  D+SVS+     +CQ    F++ L+EGN
Sbjct: 381 LGNGKMLAGMGLSPPTRPHRLYTLVSANDVL-LDSSVSK-YNPSDCQRPEVFNKKLVEGN 440

Query: 454 LLICSYSIRFVLGLSTVKQALQTAKNLSATGVIFYMDSFVIGFRLNPIPMKMPGIIISSP 513
           +L+C YS  FV+G +++K+ + TAK+L A G +  +++   G + +P+P  +PGI+I+  
Sbjct: 441 ILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPGILITDV 500

Query: 514 EDSKMLLQYYNSSLEVDGLTKKISKFGAVASICGGLKANYSSSAPQIMYYSARGPDPEDS 573
             S  L+ YYN+S   D  T ++  F A  SI  GL      SAPQ+  +SARGP+ +D 
Sbjct: 501 SKSMDLIDYYNASTSRD-WTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGPNTKDF 560

The following BLAST results are available for this feature:

BLAST of IVF0013556 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FI12	2.8e-215	65.05	Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S...	[more]
Q9SUN6	4.9e-212	63.34	Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S...	[more]
Q9SA75	1.3e-196	60.71	Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 S...	[more]
O64481	1.4e-121	44.29	Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S...	[more]
Q9SZV5	2.0e-120	42.53	Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S...	[more]

BLAST of IVF0013556 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3AZJ6	0.0e+00	94.21	subtilisin-like protease SBT2.3 OS=Cucumis melo OX=3656 GN=LOC103484283 PE=3 SV=...	[more]
A0A0A0KMB5	0.0e+00	92.55	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G526310 PE=3 SV=1	[more]
A0A5A7U976	0.0e+00	94.21	Subtilisin-like protease SBT2.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A6J1IJ51	1.3e-303	89.27	subtilisin-like protease SBT2.3 OS=Cucurbita maxima OX=3661 GN=LOC111477908 PE=3...	[more]
A0A6J1EDC1	2.8e-303	88.94	subtilisin-like protease SBT2.2 OS=Cucurbita moschata OX=3662 GN=LOC111433134 PE...	[more]

BLAST of IVF0013556 vs. NCBI nr


Match Name	Identity	Description
XP_008439503.1	94.21	PREDICTED: subtilisin-like protease SBT2.3 [Cucumis melo]	[more]
XP_011658335.1	92.55	subtilisin-like protease SBT2.3 [Cucumis sativus] >KGN49497.1 hypothetical prote...	[more]
XP_038883866.1	91.25	subtilisin-like protease SBT2.2 [Benincasa hispida]	[more]
KAA0052453.1	94.21	subtilisin-like protease SBT2.3 [Cucumis melo var. makuwa] >TYK13369.1 subtilisi...	[more]
XP_023543118.1	89.11	subtilisin-like protease SBT2.2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of IVF0013556 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44530.1	2.0e-216	65.05	Subtilase family protein	[more]
AT4G20430.1	3.5e-213	63.34	Subtilase family protein	[more]
AT4G20430.2	5.4e-198	60.49	Subtilase family protein	[more]
AT1G30600.1	9.3e-198	60.71	Subtilase family protein	[more]
AT2G19170.1	9.8e-123	44.29	subtilisin-like serine protease 3	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015500	Peptidase S8, subtilisin-related	PRINTS	PR00723	SUBTILISIN	coord: 169..188 score: 40.83 coord: 236..249 score: 49.79
None	No IPR available	GENE3D	3.50.30.30		coord: 356..524 e-value: 6.3E-68 score: 231.6
None	No IPR available	PANTHER	PTHR10795:SF539	TRIPEPTIDYL-PEPTIDASE II-RELATED	coord: 20..575
None	No IPR available	PROSITE	PS51892	SUBTILASE	coord: 149..576 score: 14.227468
None	No IPR available	CDD	cd02120	PA_subtilisin_like	coord: 362..497 e-value: 5.05805E-13 score: 63.9719
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 34..144 e-value: 5.5E-21 score: 75.0
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 22..142 e-value: 2.2E-8 score: 36.4
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 171..572 e-value: 6.3E-68 score: 231.6
IPR036852	Peptidase S8/S53 domain superfamily	SUPERFAMILY	52743	Subtilisin-like	coord: 144..568
IPR000209	Peptidase S8/S53 domain	PFAM	PF00082	Peptidase_S8	coord: 169..572 e-value: 4.2E-15 score: 55.8
IPR045051	Subtilisin-like protease	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 20..575
IPR023827	Peptidase S8, subtilisin, Asp-active site	PROSITE	PS00136	SUBTILASE_ASP	coord: 174..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0013556.2	IVF0013556.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004252	serine-type endopeptidase activity
molecular_function	GO:0008236	serine-type peptidase activity