IVF0013551 (gene) Melon (IVF77) v1

BLAST of IVF0013551 vs. ExPASy Swiss-Prot
Match: Q9H1I8 (Activating signal cointegrator 1 complex subunit 2 OS=Homo sapiens OX=9606 GN=ASCC2 PE=1 SV=3)

HSP 1 Score: 111.7 bits (278), Expect = 4.2e-23
Identity = 140/625 (22.40%), Postives = 258/625 (41.28%), Query Frame = 0

Query: 116 ELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELS 175
           +L  LL L   +FW +V  D +L + LDS+L++  R +D        +   V+  +  L 
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLRYVPRKFD----EGVASAPEVVDMQKRLH 124

Query: 176 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 235
           R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L +
Sbjct: 125 RSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQ 184

Query: 236 ILVDNAIKSQPS----IHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKL 295
            ++ N    QPS    + ETLP+++  F +I+     +   +  T       G      +
Sbjct: 185 KMIGNIFTQQPSYYSDLDETLPTILQVFSNILQHCGLQGDGANTTPQKLEERGRLTPSDM 244

Query: 296 Q-ADFLEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPS 355
              +  +++ ++ D    L +F+  + LA   F           D    LA  ++  +P 
Sbjct: 245 PLLELKDIVLYLCDTCTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEAAIPE 304

Query: 356 LQQGFQIVLMPQGDEMISNVATSLKMLALRIVS-FGWNLLEICYLG------DEVFGNDL 415
           ++   +   + +  +++ ++   L     +++  F   L +IC L       D + G   
Sbjct: 305 MESAIKKRRL-EDSKLLGDLWQRLSHSRKKLMEIFHIILNQICLLPILESSCDNIQGF-- 364

Query: 416 PVPVSMKMFPANVEDP--VIRADILIQTLREINGISQQASGKQLGQT--FLQRMEKNHSI 475
            +   +++F + +++   +   D L     +I+ + Q +S     +T   LQ +E     
Sbjct: 365 -IEEFLQIFSSLLQEKRFLRDYDALFPVAEDISLLQQASSVLDETRTAYILQAVE----- 424

Query: 476 MNRINSLRNNGWIFVDDEQFNYLSTMVMYTPTSDTKDPSLSKAP---------------- 535
                    + W  VD  +             +D KDPS+ + P                
Sbjct: 425 ---------SAWEGVDRRK------------ATDAKDPSVIEEPNGEPNGVTVTAEAVSQ 484

Query: 536 MVSHVSEVDEDAAM--------------LESKICQIKDLFPEYGSGFLAACLVAYNQNPE 595
             SH    +E+  M              L+S I Q+KDL P+ G GF+ ACL  Y+ +PE
Sbjct: 485 ASSHPENSEEEECMGAAAAVGPAMCGVELDSLISQVKDLLPDLGEGFILACLEYYHYDPE 544

Query: 596 EVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTVPYTD--QVS 655
           +VI  ILE  L P L  LD +L+    P+ +    +R +  +   F+  +    D  +V 
Sbjct: 545 QVINNILEERLAPTLSQLDRNLDREMKPDPTPLLTSRHNVFQNDEFDVFSRDSVDLSRVH 604

Query: 656 RGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYEYEDEYDD 693
           +GK    E  + S  +  R V   +        +          +    S Y YEDEYDD
Sbjct: 605 KGKSTRKEENTRSLLNDKRAVAAQRQRYEQYSVVVEEVPLQPGESLPYHSVY-YEDEYDD 641

BLAST of IVF0013551 vs. ExPASy Swiss-Prot
Match: Q91WR3 (Activating signal cointegrator 1 complex subunit 2 OS=Mus musculus OX=10090 GN=Ascc2 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.0e-21
Identity = 148/628 (23.57%), Postives = 265/628 (42.20%), Query Frame = 0

Query: 116 ELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELS 175
           +L  LL L   +FW +V  D +L + LDS+L +  R +D      T  VA +   +  L 
Sbjct: 65  DLDWLLALPHDKFWCQVIFDETLQKCLDSYLHYVPRKFD-EWVAPTPEVADM---QNHLH 124

Query: 176 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 235
           R VF+   RMS++++        +S    G +L    L D+PK+LD+C ++   N  L +
Sbjct: 125 RSVFLTFLRMSTHKE---SKDHFISPSAFGEILYNNFLFDIPKILDLCVLFGKGNSPLLQ 184

Query: 236 ILVDNAIKSQPS----IHETLPSVISHFLSIV---SMMHERCSSSLETLFSSSSHGGSGY 295
            ++ N    QPS    + ET+P+++  F +I+    +  +  S++ + L   S    S  
Sbjct: 185 KMIGNIFTQQPSYYTDLDETIPTILQVFSNILQHCGLQGDGTSTTPQKLGERSPLTPSDM 244

Query: 296 GKLQADFLEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLL 355
             L  +  +++ ++ D    L +F+  + LA   F           D    LA  +++ +
Sbjct: 245 PLL--ELKDIVLYLCDTSTTLWAFLDIFPLACQTFQ--------KHDFCYRLASFYEMAI 304

Query: 356 PSLQQGFQIVLMPQG---DEMISNVATSLKMLALRIVSFGWNLLEICYLG------DEVF 415
           P L+   +   +       +M   ++ S K L   +  F   L +IC L       D + 
Sbjct: 305 PELESAIKKRRLEDSKLLGDMWQRLSHSKKKL---MEVFHIILNQICLLPILESSCDNIQ 364

Query: 416 GNDLPVPVSMKMFPANVEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHS- 475
           G    +   +++F + +++         + LR+ +  S  A    L Q     +++  + 
Sbjct: 365 GF---IEEFLQIFSSLLQEK--------RFLRDYDTFSPVAEDISLLQQASSALDETRTA 424

Query: 476 -IMNRINSLRNNGWIFVDDEQFNYLSTMVMYTPTSDTKDPSLSK-------------APM 535
            I+  + S     W  VD ++              D KDP  +K             + M
Sbjct: 425 YILQAVES----AWEGVDRQKI------------KDIKDPPRAKGSNNEVTVTAEPVSEM 484

Query: 536 VSHVSEVDED-------AAM--------LESKICQIKDLFPEYGSGFLAACLVAYNQNPE 595
            S +  ++ED       AA+        L+S I Q+KDL P+ G GF+ ACL  Y+ + E
Sbjct: 485 PSQLENLEEDEECMGAAAALGPAVSGVELDSLISQVKDLLPDLGEGFILACLEHYSYDSE 544

Query: 596 EVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTVPYTD--QVS 655
           +VI  ILE  L P+L  LD  LE    P+ +    +R +  +   F+  +    D  +V 
Sbjct: 545 QVINNILEDRLAPELSQLDRGLERQVKPDPTPLLSSRHNIFQNDEFDVFSRDSVDLSRVH 604

Query: 656 RGKDLPSEGPSV---SSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYEYEDE 693
           +G+       S+       V ++ R  K  V   E      E  +          +YEDE
Sbjct: 605 KGRRKEENVRSLVNDKQAVVAQWQRYQKYSVVVEEVPLQPGEYQA---------DDYEDE 634

BLAST of IVF0013551 vs. ExPASy Swiss-Prot
Match: Q54VC4 (Activating signal cointegrator 1 complex subunit 2 homolog OS=Dictyostelium discoideum OX=44689 GN=ascc2 PE=3 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 3.8e-16
Identity = 151/708 (21.33%), Postives = 277/708 (39.12%), Query Frame = 0

Query: 79  SFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRLLKLSAKEFWREVATDTSL 138
           SF+ +LP D         E+G+     S+  +  +N +LS LLK     FW     + SL
Sbjct: 102 SFITFLPAD-------NNEQGS-----SEESILFMNSDLSSLLKFQYNIFWSHCIFNKSL 161

Query: 139 HEFLDSFLKFRTR---------WYDFPHRGATGTVAGVIVGEYE-------LSRRVFMAL 198
           +EF+DSFLKF  R           +  +       + +  G  +       L +RVF+ L
Sbjct: 162 NEFIDSFLKFFKRDNQPIISLVNNNSVNNNNNNNNSIIKKGSKDFNESKKLLFKRVFLVL 221

Query: 199 YRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDNAI 258
            RMS  ++     +  ++ + +  L+ + KL  +PKL DI ++YS    D    ++ +  
Sbjct: 222 VRMSLQQE----KSGFITREFYSDLIYKNKLFTIPKLFDIVSLYSSHARDAVTTMIQSIF 281

Query: 259 KSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID-F 318
            +QP+ ++ L   + HF  I   + E   S L               +   D L + D +
Sbjct: 282 DTQPNYYKDL---LQHFQLISKTLFELNQSLL--------------NRNLLDLLRLEDNY 341

Query: 319 INDAIVNLDSFVAAYRLAA--IFFSSAVEISCGNED----LLGMLARLHDLLLPSLQQGF 378
           + D + NL+ F+  + + +  +F    + +  G  D    +LG L   ++ ++P   +  
Sbjct: 342 LMDIVYNLEQFIRIFPMGSHQLFDEYLMNLEKGGGDGNGGVLGWLTYFYEYIIPIFNKEN 401

Query: 379 Q-----------IVLMPQGDEMISNVATSLK-MLALRIVSFGWNLLEICYLGDEVFGNDL 438
           Q            + +P    ++S   T  +    +++      + + C L  +     L
Sbjct: 402 QKASNKQVNLTPSIYVPLKQHILSIFHTIFRHHFMIKLEQLQLLIQDPCQLCKK-----L 461

Query: 439 PV-PVSMKMFPANVEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNR 498
           P+  +S + F            I+  T    + +  +        + L   E+ + + N 
Sbjct: 462 PLDEISHRFFSLLGNITSFSQQIIGSTKSNRSKLFNEYFDNITSASILYDYEQTYKLSNF 521

Query: 499 INSLRNNGWIFVDDEQFNYLSTMVMYTPTSDTKD---PSLSKAPMV--SHVSEVDEDAA- 558
           ++ L       +D   + Y   ++    + D K    P   K+  +  S++S     ++ 
Sbjct: 522 LDKLVTLD-SSIDFTSYTYFMQLIGKPVSQDQKQIQKPKFKKSTTIIDSNISSSSSSSSS 581

Query: 559 --------------------MLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRIL 618
                               M   KI Q+K LFP+ G  F+  CL  YNQ+ E+VI  + 
Sbjct: 582 SSPSNATTTVKPILSNNPVIMNTVKIDQVKSLFPDLGDWFVHCCLKYYNQDVEQVINALC 641

Query: 619 -EGTLHPDLQSLDTSL--------ETMPVPNSSATAINRKDKGKGKLFEPSTVPYTDQVS 678
            + +L P L+S+D SL        + +P P ++ T     DK        +T   T    
Sbjct: 642 DDSSLPPHLKSMDRSLSSDPSQPSKNIPTPTTTTTTTTTTDKPNNTTTATTTTTTT---- 701

Query: 679 RGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYEYEDEYDD 714
                     + SS S+ + + +    + Y +  D + + DS+   +  S  + E   D+
Sbjct: 702 ----------TSSSKSLEKSMNELNISIGYEKIYDEKYD-DSLEEFSGFSVQDGEKYNDE 751

BLAST of IVF0013551 vs. ExPASy TrEMBL
Match: A0A1S4DZ72 (uncharacterized protein LOC103493893 OS=Cucumis melo OX=3656 GN=LOC103493893 PE=4 SV=1)

HSP 1 Score: 1679.5 bits (4348), Expect = 0.0e+00
Identity = 869/869 (100.00%), Postives = 869/869 (100.00%), Query Frame = 0

Query: 1    MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
            MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1385 MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 1444

Query: 61   RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
            RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 1445 RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 1504

Query: 121  LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
            LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 1505 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 1564

Query: 181  ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
            ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN
Sbjct: 1565 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 1624

Query: 241  AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
            AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 1625 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 1684

Query: 301  FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
            FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 1685 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 1744

Query: 361  PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
            PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 1745 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 1804

Query: 421  IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
            IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 1805 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 1864

Query: 481  YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
            YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 1865 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 1924

Query: 541  LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
            LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 1925 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 1984

Query: 601  PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
            PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 1985 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 2044

Query: 661  YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
            YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 2045 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 2104

Query: 721  TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
            TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 2105 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 2164

Query: 781  QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
            QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 2165 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 2224

Query: 841  NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 870
            NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 2225 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 2253

BLAST of IVF0013551 vs. ExPASy TrEMBL
Match: A0A5A7VB30 (Activating signal cointegrator 1 complex subunit 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001520 PE=4 SV=1)

HSP 1 Score: 1659.8 bits (4297), Expect = 0.0e+00
Identity = 861/869 (99.08%), Postives = 862/869 (99.19%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILV  
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVII 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
            + S   IHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 241 FLSSSYCIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 870
           NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869

BLAST of IVF0013551 vs. ExPASy TrEMBL
Match: A0A5D3D9H3 (Activating signal cointegrator 1 complex subunit 2-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G002760 PE=4 SV=1)

HSP 1 Score: 1657.5 bits (4291), Expect = 0.0e+00
Identity = 861/869 (99.08%), Postives = 861/869 (99.08%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRIL   
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRIL--- 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
                PSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 241 -----PSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 870
           NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 861

BLAST of IVF0013551 vs. ExPASy TrEMBL
Match: A0A0A0L0H3 (CUE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G642490 PE=4 SV=1)

HSP 1 Score: 1589.7 bits (4115), Expect = 0.0e+00
Identity = 822/869 (94.59%), Postives = 841/869 (96.78%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQST+ELPNPKPTLSTSL+QSLPNPSDSTAAPS+S
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTNELPNPKPTLSTSLKQSLPNPSDSTAAPSMS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKL+AKEFWREVA DTSLHEFLDSFLKFRTRWYDFPHRGA GTVAGVIVGE ELSRRVFM
Sbjct: 121 LKLNAKEFWREVAMDTSLHEFLDSFLKFRTRWYDFPHRGANGTVAGVIVGENELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
           AIKSQPSIH+TLPSVISHFL IVSMMHERCSSSLETLFSSSSHGGSGY KLQADFLEVID
Sbjct: 241 AIKSQPSIHQTLPSVISHFLRIVSMMHERCSSSLETLFSSSSHGGSGYSKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIV+LDSFV AYRLAAIFF SAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVSLDSFVTAYRLAAIFFCSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGW LLEICYL DEVFGNDLP+PVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWKLLEICYLDDEVFGNDLPIPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQAS KQLGQTFLQ MEKNHS MNRINSLR  GW+FVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASDKQLGQTFLQHMEKNHSTMNRINSLRKKGWMFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLST+VMYTPTS  KDPSLSKAPM+SH+SEVDEDAAMLESKICQIKDLFPEYGSGF+AAC
Sbjct: 481 YLSTIVMYTPTSGIKDPSLSKAPMISHISEVDEDAAMLESKICQIKDLFPEYGSGFVAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLH DL SLDTSLETMPVPNSSATA NRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHVDLLSLDTSLETMPVPNSSATANNRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLD+RNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDSRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQ+PSSHL SS NSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQRPSSHLSSSMNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGS+AVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSIAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQP  + VDPRDNVRK+WGRGR  RERERE GAAPGMPEGEGKQPNVAE SDRGGRGG
Sbjct: 781 QDSQPDVSAVDPRDNVRKSWGRGR--REREREGGAAPGMPEGEGKQPNVAEVSDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 870
           NRGRGRRGGGDHHRKDRA++KHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAMKKHFAGLSGF 867

BLAST of IVF0013551 vs. ExPASy TrEMBL
Match: A0A6J1FF14 (activating signal cointegrator 1 complex subunit 2-like OS=Cucurbita moschata OX=3662 GN=LOC111443354 PE=4 SV=1)

HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 760/876 (86.76%), Postives = 806/876 (92.01%), Query Frame = 0

Query: 1   MSNRYNHDG-NKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSD-----ST 60
           MSNRYNHDG NKGLRK+QKKYIPKNQIQST+E PNPKP LSTSLRQSLP PSD     S+
Sbjct: 1   MSNRYNHDGNNKGLRKEQKKYIPKNQIQSTNERPNPKPNLSTSLRQSLPKPSDSAAVSSS 60

Query: 61  AAPSVSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLN 120
           A PS+SRIQMGANGDWVSSRA+GGSFVNYLPQDEAVATGLRAEEGALDP+ESQRVVDLLN
Sbjct: 61  ATPSMSRIQMGANGDWVSSRATGGSFVNYLPQDEAVATGLRAEEGALDPMESQRVVDLLN 120

Query: 121 RELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYEL 180
           RELSRLLKLSAKEFW EVA DTSLHEFLDSFLKFR+RWYDFPHRGA G VAGVIVGE EL
Sbjct: 121 RELSRLLKLSAKEFWTEVARDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGECEL 180

Query: 181 SRRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLT 240
           SRRVFM LYRMSSNRDPGARAADSLSLKDHGVLLQ KKLLDLPKLLDICAIY HENEDLT
Sbjct: 181 SRRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYYHENEDLT 240

Query: 241 RILVDNAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQAD 300
           R LV+NAIKSQPSIHETLPSVISHFLSIVSMMH+RC+SSLETLFSSSS G SGY KLQAD
Sbjct: 241 RTLVENAIKSQPSIHETLPSVISHFLSIVSMMHQRCNSSLETLFSSSSSGESGYSKLQAD 300

Query: 301 FLEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQG 360
           FLEVIDFINDAIV LDSFVAAYRLAAIFFSSAVEISCGNEDLLG LARLHDLLLPSLQQG
Sbjct: 301 FLEVIDFINDAIVTLDSFVAAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPSLQQG 360

Query: 361 FQIVLMPQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPA 420
           FQIV +P+GD+M+S+V TSLKMLALR+VS GW LLEICYLGDEVFGNDL VPVSMKMFPA
Sbjct: 361 FQIVFVPRGDDMMSSVVTSLKMLALRVVSLGWKLLEICYLGDEVFGNDLAVPVSMKMFPA 420

Query: 421 NVEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFV 480
           NVEDPVIRADI IQTLREINGISQQA  KQLGQTFLQRMEKNHS+MNRINSLRNNGWIFV
Sbjct: 421 NVEDPVIRADIFIQTLREINGISQQAPDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFV 480

Query: 481 DDEQFNYLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGS 540
           DDEQF+Y+S +V YTPT + KD SLSKAP++SH+SEVDED+AMLESKICQIKDLFPEYGS
Sbjct: 481 DDEQFDYISKIV-YTPTPNIKDSSLSKAPVMSHISEVDEDSAMLESKICQIKDLFPEYGS 540

Query: 541 GFLAACLVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKL 600
           GFLAACLVAYNQNPEEVIQRILEGTLH DLQSLDTSLETMPVPNSSA AINR DKGKGKL
Sbjct: 541 GFLAACLVAYNQNPEEVIQRILEGTLHADLQSLDTSLETMPVPNSSAAAINRNDKGKGKL 600

Query: 601 FEPSTVPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAA 660
           FE STV YTDQVS+ KDL  EGPSVSST  GR+VRKSKDD+PYSETLD+RNEADSVRTA+
Sbjct: 601 FESSTVAYTDQVSQSKDLMVEGPSVSSTFSGRYVRKSKDDMPYSETLDSRNEADSVRTAS 660

Query: 661 LISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNS 720
           L+SQYEYEDEYDDSFDDLGISIAETATEDNEDLV QK SS LG+SSNS N SSAQNA NS
Sbjct: 661 LVSQYEYEDEYDDSFDDLGISIAETATEDNEDLVSQKLSSDLGNSSNSKNASSAQNASNS 720

Query: 721 KWGSRRTPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVK 780
           KWGS+R PQYYVKDGKNYSYKVAGS+AV+NSDEASLVTQAQKELI+GLGRGGNLPLGAVK
Sbjct: 721 KWGSKRKPQYYVKDGKNYSYKVAGSIAVSNSDEASLVTQAQKELIHGLGRGGNLPLGAVK 780

Query: 781 KLTESQQDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASD 840
           KLTES+QDSQP  +  DPRDNVRK+WGRG R   RE  SG+A G+PEG+GKQPNVAE S+
Sbjct: 781 KLTESEQDSQPDVSAADPRDNVRKSWGRGSR---REVGSGSAAGVPEGQGKQPNVAEVSE 840

Query: 841 RGGRGGNRGRGRRG-GGDHHRKDRAIRKHFAGLSGF 870
           R GRGGNRGRGR G  G+HHRKDRA++KHFAGLSGF
Sbjct: 841 RRGRGGNRGRGRGGASGNHHRKDRAMKKHFAGLSGF 872

BLAST of IVF0013551 vs. NCBI nr
Match: XP_016901291.1 (PREDICTED: uncharacterized protein LOC103493893 [Cucumis melo])

HSP 1 Score: 1681 bits (4353), Expect = 0.0
Identity = 869/869 (100.00%), Postives = 869/869 (100.00%), Query Frame = 0

Query: 1    MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
            MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1385 MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 1444

Query: 61   RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
            RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 1445 RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 1504

Query: 121  LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
            LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 1505 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 1564

Query: 181  ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
            ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN
Sbjct: 1565 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 1624

Query: 241  AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
            AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 1625 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 1684

Query: 301  FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
            FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 1685 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 1744

Query: 361  PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
            PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 1745 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 1804

Query: 421  IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
            IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 1805 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 1864

Query: 481  YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
            YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 1865 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 1924

Query: 541  LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
            LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 1925 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 1984

Query: 601  PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
            PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 1985 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 2044

Query: 661  YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
            YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 2045 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 2104

Query: 721  TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
            TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 2105 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 2164

Query: 781  QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
            QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 2165 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 2224

Query: 841  NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869
            NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 2225 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 2253

BLAST of IVF0013551 vs. NCBI nr
Match: KAA0064440.1 (activating signal cointegrator 1 complex subunit 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1661 bits (4302), Expect = 0.0
Identity = 861/869 (99.08%), Postives = 862/869 (99.19%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILV  
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVII 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
            + S   IHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 241 FLSSSYCIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869
           NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869

BLAST of IVF0013551 vs. NCBI nr
Match: TYK20149.1 (activating signal cointegrator 1 complex subunit 2-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1659 bits (4297), Expect = 0.0
Identity = 861/869 (99.08%), Postives = 861/869 (99.08%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM
Sbjct: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRIL   
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRIL--- 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
                PSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID
Sbjct: 241 -----PSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC
Sbjct: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG
Sbjct: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869
           NRGRGRRGGGDHHRKDRAIRKHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 861

BLAST of IVF0013551 vs. NCBI nr
Match: XP_031739759.1 (activating signal cointegrator 1 complex subunit 2 isoform X1 [Cucumis sativus] >XP_031739760.1 activating signal cointegrator 1 complex subunit 2 isoform X1 [Cucumis sativus] >KGN55268.1 hypothetical protein Csa_012201 [Cucumis sativus])

HSP 1 Score: 1591 bits (4120), Expect = 0.0
Identity = 822/869 (94.59%), Postives = 841/869 (96.78%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDSTAAPSVS 60
           MSNRYNHDGNKGLRKDQKKYIPKNQIQST+ELPNPKPTLSTSL+QSLPNPSDSTAAPS+S
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTNELPNPKPTLSTSLKQSLPNPSDSTAAPSMS 60

Query: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120
           RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL
Sbjct: 61  RIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNRELSRL 120

Query: 121 LKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELSRRVFM 180
           LKL+AKEFWREVA DTSLHEFLDSFLKFRTRWYDFPHRGA GTVAGVIVGE ELSRRVFM
Sbjct: 121 LKLNAKEFWREVAMDTSLHEFLDSFLKFRTRWYDFPHRGANGTVAGVIVGENELSRRVFM 180

Query: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240
           ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN
Sbjct: 181 ALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTRILVDN 240

Query: 241 AIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADFLEVID 300
           AIKSQPSIH+TLPSVISHFL IVSMMHERCSSSLETLFSSSSHGGSGY KLQADFLEVID
Sbjct: 241 AIKSQPSIHQTLPSVISHFLRIVSMMHERCSSSLETLFSSSSHGGSGYSKLQADFLEVID 300

Query: 301 FINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360
           FINDAIV+LDSFV AYRLAAIFF SAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM
Sbjct: 301 FINDAIVSLDSFVTAYRLAAIFFCSAVEISCGNEDLLGMLARLHDLLLPSLQQGFQIVLM 360

Query: 361 PQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPANVEDPV 420
           PQGDEMISNVATSLKMLALRIVSFGW LLEICYL DEVFGNDLP+PVSMKMFPANVEDPV
Sbjct: 361 PQGDEMISNVATSLKMLALRIVSFGWKLLEICYLDDEVFGNDLPIPVSMKMFPANVEDPV 420

Query: 421 IRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFN 480
           IRADILIQTLREINGISQQAS KQLGQTFLQ MEKNHS MNRINSLR  GW+FVDDEQFN
Sbjct: 421 IRADILIQTLREINGISQQASDKQLGQTFLQHMEKNHSTMNRINSLRKKGWMFVDDEQFN 480

Query: 481 YLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSGFLAAC 540
           YLST+VMYTPTS  KDPSLSKAPM+SH+SEVDEDAAMLESKICQIKDLFPEYGSGF+AAC
Sbjct: 481 YLSTIVMYTPTSGIKDPSLSKAPMISHISEVDEDAAMLESKICQIKDLFPEYGSGFVAAC 540

Query: 541 LVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLFEPSTV 600
           LVAYNQNPEEVIQRILEGTLH DL SLDTSLETMPVPNSSATA NRKDKGKGKLFEPSTV
Sbjct: 541 LVAYNQNPEEVIQRILEGTLHVDLLSLDTSLETMPVPNSSATANNRKDKGKGKLFEPSTV 600

Query: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYE 660
           PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLD+RNEADSVRTAALISQYE
Sbjct: 601 PYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDSRNEADSVRTAALISQYE 660

Query: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRR 720
           YEDEYDDSFDDLGISIAETATEDNEDLVGQ+PSSHL SS NSTNGSSAQNAPNSKWGSRR
Sbjct: 661 YEDEYDDSFDDLGISIAETATEDNEDLVGQRPSSHLSSSMNSTNGSSAQNAPNSKWGSRR 720

Query: 721 TPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780
           TPQYYVKDGKNYSYKVAGS+AVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ
Sbjct: 721 TPQYYVKDGKNYSYKVAGSIAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTESQ 780

Query: 781 QDSQPASAVVDPRDNVRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASDRGGRGG 840
           QDSQP  + VDPRDNVRK+WGRGRRERERE   GAAPGMPEGEGKQPNVAE SDRGGRGG
Sbjct: 781 QDSQPDVSAVDPRDNVRKSWGRGRRERERE--GGAAPGMPEGEGKQPNVAEVSDRGGRGG 840

Query: 841 NRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869
           NRGRGRRGGGDHHRKDRA++KHFAGLSGF
Sbjct: 841 NRGRGRRGGGDHHRKDRAMKKHFAGLSGF 867

BLAST of IVF0013551 vs. NCBI nr
Match: XP_038897592.1 (activating signal cointegrator 1 complex subunit 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 1509 bits (3908), Expect = 0.0
Identity = 789/875 (90.17%), Postives = 819/875 (93.60%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLRKDQKKYIPKNQIQSTHELPNPKPTLSTSLRQSLPNPSDS-----TA 60
           MSNRYNHDGNKGLRKDQKKYIPKNQ QST+ELPNPKPTLS+SLRQSLP PSDS     +A
Sbjct: 1   MSNRYNHDGNKGLRKDQKKYIPKNQNQSTNELPNPKPTLSSSLRQSLPKPSDSAAVTTSA 60

Query: 61  APSVSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEEGALDPVESQRVVDLLNR 120
           APS SRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAE GALDP+ESQRVVDLLNR
Sbjct: 61  APSTSRIQMGANGDWVSSRASGGSFVNYLPQDEAVATGLRAEAGALDPMESQRVVDLLNR 120

Query: 121 ELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIVGEYELS 180
           ELSRLLKLSAKEFWREVA DTSLHEFLDSFLKFR+RWYDFPHRGA G VAGVIVGEYELS
Sbjct: 121 ELSRLLKLSAKEFWREVAMDTSLHEFLDSFLKFRSRWYDFPHRGANGIVAGVIVGEYELS 180

Query: 181 RRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHENEDLTR 240
           RRVFM LYRMSSNRDPGARAADSLSLKDHGVLLQ KKLLDLPKLLDICAIYSHENEDLTR
Sbjct: 181 RRVFMVLYRMSSNRDPGARAADSLSLKDHGVLLQGKKLLDLPKLLDICAIYSHENEDLTR 240

Query: 241 ILVDNAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYGKLQADF 300
           ILV+NAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSS+HGGSGY KLQADF
Sbjct: 241 ILVENAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSNHGGSGYSKLQADF 300

Query: 301 LEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLPSLQQGF 360
           LEVIDFINDAIV LDSFV AYRLAAIFFSSAVEISCGNEDLLG LARLHDLLLP L +GF
Sbjct: 301 LEVIDFINDAIVTLDSFVTAYRLAAIFFSSAVEISCGNEDLLGTLARLHDLLLPYLHKGF 360

Query: 361 QIVLMPQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSMKMFPAN 420
           QIVL+PQGDEM+SNVATSLKMLALRIVS GW LLEICYL DEVFG+ LPVPVSMKMFPAN
Sbjct: 361 QIVLVPQGDEMLSNVATSLKMLALRIVSLGWKLLEICYLDDEVFGHALPVPVSMKMFPAN 420

Query: 421 VEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNNGWIFVD 480
           VEDPVIRADILIQTLREINGISQQAS KQLGQTFLQRMEKNHS+MNRINSLRNNGWIFVD
Sbjct: 421 VEDPVIRADILIQTLREINGISQQASDKQLGQTFLQRMEKNHSVMNRINSLRNNGWIFVD 480

Query: 481 DEQFNYLSTMVMYTPTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDLFPEYGSG 540
           DEQF+YLST+VMYTPTSD K+PSLSKAP++SH+SEVDEDAAMLESKICQIKDLFPEYGSG
Sbjct: 481 DEQFDYLSTIVMYTPTSDVKNPSLSKAPVMSHISEVDEDAAMLESKICQIKDLFPEYGSG 540

Query: 541 FLAACLVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKDKGKGKLF 600
           FLAACL AYNQNPEEVI+RILEGTLH DLQSLDTSLET PVPNS ATA NR DKGKGKLF
Sbjct: 541 FLAACLEAYNQNPEEVIERILEGTLHSDLQSLDTSLETKPVPNSRATANNRNDKGKGKLF 600

Query: 601 EPSTVPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDNRNEADSVRTAAL 660
           E  TV Y DQVSR KDL SEGPSVSSTSVGRFVRKSKD++PYSETLDNRNEAD VRTAAL
Sbjct: 601 EFPTVAYIDQVSRDKDLTSEGPSVSSTSVGRFVRKSKDNMPYSETLDNRNEADPVRTAAL 660

Query: 661 ISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSK 720
           ISQYEYEDEYDDSFDDLGISIAET TEDNED +GQK SS+ GSSSNSTNGSSA NAPNSK
Sbjct: 661 ISQYEYEDEYDDSFDDLGISIAETTTEDNEDSIGQKLSSNSGSSSNSTNGSSASNAPNSK 720

Query: 721 WGSRRTPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780
           WGSRR PQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK
Sbjct: 721 WGSRRKPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLGRGGNLPLGAVKK 780

Query: 781 LTESQQDSQPASAVVDPRDN-VRKTWGRGRRERERERESGAAPGMPEGEGKQPNVAEASD 840
           LTE++QDSQP  +  DPRDN VRK+WGRGRRERE    SG APGMPEG+GKQPNVAE SD
Sbjct: 781 LTEAEQDSQPDVSAGDPRDNNVRKSWGRGRRERE----SGTAPGMPEGQGKQPNVAEVSD 840

Query: 841 RGGRGGNRGRGRRGGGDHHRKDRAIRKHFAGLSGF 869
           RGGRGGNRGRGRRGGGDHHRKDRA++KHFAGLSGF
Sbjct: 841 RGGRGGNRGRGRRGGGDHHRKDRAMKKHFAGLSGF 871

BLAST of IVF0013551 vs. TAIR 10
Match: AT1G27752.1 (Ubiquitin system component Cue protein )

HSP 1 Score: 835.1 bits (2156), Expect = 5.1e-242
Identity = 489/902 (54.21%), Postives = 635/902 (70.40%), Query Frame = 0

Query: 1   MSNRYNHDGNKGLR-----KDQKKYIPK--NQIQSTHELPNPKPTLSTSLRQS-LPNPSD 60
           MSNR ++  ++  R       Q+K++PK  N   +++  P P  +LS+SLRQS     S 
Sbjct: 1   MSNRRSNRQDENTRYVPKGHQQQKFVPKPMNPTPTSNSTPFP-VSLSSSLRQSDSSGASS 60

Query: 61  STAAPSVSRIQMGANGDWVSSRA---SGGSFVNYLPQDEAVATGLRAEEGALDPVESQRV 120
             +A   SR+++G  G  VSS++    GGSFVNYLPQDEAVA GL  ++G LDPVESQ V
Sbjct: 61  RVSASGGSRVRIGDQGQLVSSKSPAQGGGSFVNYLPQDEAVAAGLGPDDGGLDPVESQGV 120

Query: 121 VDLLNRELSRLLKLSAKEFWREVATDTSLHEFLDSFLKFRTRWYDFPHRGATGTVAGVIV 180
           VDLLNREL+RLLKL+ ++FWREVA+D SLH+FLDSFL+FR+RWYDFP  G  G VAGVIV
Sbjct: 121 VDLLNRELTRLLKLNPRDFWREVASDASLHDFLDSFLQFRSRWYDFPFHGVKGIVAGVIV 180

Query: 181 GEYELSRRVFMALYRMSSNRDPGARAADSLSLKDHGVLLQEKKLLDLPKLLDICAIYSHE 240
           GE EL RRVFM LYR+SSNRDPGA+AADSLS KDH VLLQ+KKLLDLPKLLDICAIY HE
Sbjct: 181 GELELCRRVFMVLYRISSNRDPGAKAADSLSQKDHEVLLQDKKLLDLPKLLDICAIYGHE 240

Query: 241 NEDLTRILVDNAIKSQPSIHETLPSVISHFLSIVSMMHERCSSSLETLFSSSSHGGSGYG 300
           N +LT+ L++NA+KSQ  I E+L  ++SHFL I+  MH RC+SSLETL SS++    G  
Sbjct: 241 NAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMHHRCTSSLETLVSSANSEDHGRR 300

Query: 301 KLQADFLEVIDFINDAIVNLDSFVAAYRLAAIFFSSAVEISCGNEDLLGMLARLHDLLLP 360
           +L +D LEV+DFIND +V+LD+F++AY  A    +  VE S G+++LL  L RLHD LLP
Sbjct: 301 QLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPVETSYGSDELLRSLVRLHDSLLP 360

Query: 361 SLQQGFQIVLMPQGDEMISNVATSLKMLALRIVSFGWNLLEICYLGDEVFGNDLPVPVSM 420
           SL +GFQ++   +  + +S+++TSL ML+ RI S  W +L+ICYL ++ F ++  +P   
Sbjct: 361 SLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWKILDICYLSNDKFNHETSIPAVT 420

Query: 421 KMFPANVEDPVIRADILIQTLREINGISQQASGKQLGQTFLQRMEKNHSIMNRINSLRNN 480
           KMFP+ VEDP++RADILIQT REI+G+S+Q+   +     LQ++EKN+ I++R+ SL+N 
Sbjct: 421 KMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--NRLLQKIEKNYRIIDRLRSLQNA 480

Query: 481 GWIFVDDEQFNYLSTMVMYT-PTSDTKDPSLSKAPMVSHVSEVDEDAAMLESKICQIKDL 540
           GWI ++DEQ  YLS +++++  T   K+  L      +    +DE+A +++SKI QIKD+
Sbjct: 481 GWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRNAEELMDENAVVMQSKISQIKDI 540

Query: 541 FPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHPDLQSLDTSLETMPVPNSSATAINRKD 600
           FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ LDTSLETMP P S+ T +  KD
Sbjct: 541 FPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQRLDTSLETMPQPKSAPT-LRSKD 600

Query: 601 KGKGKLFEPST-----VPYTDQVSRGKDLPSEGPSVSSTSVGRFVRKSKDDVPYSETLDN 660
           KGKGKL E  T       YT++      LP+   S SS +VGRFVRK KDD P  + LD 
Sbjct: 601 KGKGKLIESDTSSSASAIYTEKPITRPSLPAS--SASSATVGRFVRKPKDDTPSYKILDA 660

Query: 661 RNEADSVRTAALISQYEYEDEYDDSFDDLGISIAETATEDNEDLVGQKPSSHLGSSSNST 720
           R E+D  R AAL++QYEY+DEYDDSFDDLG+SIAE+ TE++            G+  N  
Sbjct: 661 RKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGTEES------------GAFGNRA 720

Query: 721 NGSSAQNAPNSKWGSRRTPQYYVKDGKNYSYKVAGSVAVANSDEASLVTQAQKELIYGLG 780
            GS   +AP  KWGSR+ PQ+YVKDGKNYSYKVAG+VAVAN++EASLV +A+ + I GLG
Sbjct: 721 -GSEPSDAP--KWGSRKNPQFYVKDGKNYSYKVAGAVAVANANEASLVNEAEGDKILGLG 780

Query: 781 RGGNLPLGAVKKLTE--SQQDSQPASAV-VDPRD------NVRKTWGRGR----RERERE 840
           RGGN+PLGAV+KLTE  +Q+D +  S V V+P D      N R   GRGR    RE+ +E
Sbjct: 781 RGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGRENGRNWRGVRGRGRGVVNREQPQE 840

Query: 841 RESGAAPGMPEGEGKQPNVAEASDRGGRGGNRGRGRRGGG---DHHRKDRAIRKHFAGLS 870
           + + ++    E         EA + GGRG  RGRGRRGGG   +H+ KDRA++KH A +S
Sbjct: 841 KSNESSNNNSEVN------TEAENGGGRG--RGRGRRGGGGGRNHNHKDRAMKKHIASVS 873

BLAST of IVF0013551 vs. TAIR 10
Match: AT1G27752.2 (Ubiquitin system component Cue protein )

HSP 1 Score: 609.8 bits (1571), Expect = 3.5e-174
Identity = 357/684 (52.19%), Postives = 475/684 (69.44%), Query Frame = 0

Query: 208 LQEKKLLDLPKLLDICAIYSHENEDLTRILVDNAIKSQPSIHETLPSVISHFLSIVSMMH 267
           +++KKLLDLPKLLDICAIY HEN +LT+ L++NA+KSQ  I E+L  ++SHFL I+  MH
Sbjct: 1   MKDKKLLDLPKLLDICAIYGHENAELTKSLIENAVKSQNGISESLNMMLSHFLGILHTMH 60

Query: 268 ERCSSSLETLFSSSSHGGSGYGKLQADFLEVIDFINDAIVNLDSFVAAYRLAAIFFSSAV 327
            RC+SSLETL SS++    G  +L +D LEV+DFIND +V+LD+F++AY  A    +  V
Sbjct: 61  HRCTSSLETLVSSANSEDHGRRQLHSDLLEVMDFINDGVVSLDAFISAYTPAVFILACPV 120

Query: 328 EISCGNEDLLGMLARLHDLLLPSLQQGFQIVLMPQGDEMISNVATSLKMLALRIVSFGWN 387
           E S G+++LL  L RLHD LLPSL +GFQ++   +  + +S+++TSL ML+ RI S  W 
Sbjct: 121 ETSYGSDELLRSLVRLHDSLLPSLHRGFQVLFKDEDHDSLSDISTSLNMLSTRIGSLCWK 180

Query: 388 LLEICYLGDEVFGNDLPVPVSMKMFPANVEDPVIRADILIQTLREINGISQQASGKQLGQ 447
           +L+ICYL ++ F ++  +P   KMFP+ VEDP++RADILIQT REI+G+S+Q+   +   
Sbjct: 181 ILDICYLSNDKFNHETSIPAVTKMFPSRVEDPMVRADILIQTFREISGLSEQSLESK--N 240

Query: 448 TFLQRMEKNHSIMNRINSLRNNGWIFVDDEQFNYLSTMVMYT-PTSDTKDPSLSKAPMVS 507
             LQ++EKN+ I++R+ SL+N GWI ++DEQ  YLS +++++  T   K+  L      +
Sbjct: 241 RLLQKIEKNYRIIDRLRSLQNAGWISMEDEQLQYLSMIMLHSADTFSMKESPLLLTDGRN 300

Query: 508 HVSEVDEDAAMLESKICQIKDLFPEYGSGFLAACLVAYNQNPEEVIQRILEGTLHPDLQS 567
               +DE+A +++SKI QIKD+FPEYG+GFLAACL AYNQNPEEVIQRILEGTLH DLQ 
Sbjct: 301 AEELMDENAVVMQSKISQIKDIFPEYGNGFLAACLEAYNQNPEEVIQRILEGTLHEDLQR 360

Query: 568 LDTSLETMPVPNSSATAINRKDKGKGKLFEPST-----VPYTDQVSRGKDLPSEGPSVSS 627
           LDTSLETMP P S+ T +  KDKGKGKL E  T       YT++      LP+   S SS
Sbjct: 361 LDTSLETMPQPKSAPT-LRSKDKGKGKLIESDTSSSASAIYTEKPITRPSLPAS--SASS 420

Query: 628 TSVGRFVRKSKDDVPYSETLDNRNEADSVRTAALISQYEYEDEYDDSFDDLGISIAETAT 687
            +VGRFVRK KDD P  + LD R E+D  R AAL++QYEY+DEYDDSFDDLG+SIAE+ T
Sbjct: 421 ATVGRFVRKPKDDTPSYKILDARKESDRERNAALLAQYEYDDEYDDSFDDLGLSIAESGT 480

Query: 688 EDNEDLVGQKPSSHLGSSSNSTNGSSAQNAPNSKWGSRRTPQYYVKDGKNYSYKVAGSVA 747
           E++            G+  N   GS   +AP  KWGSR+ PQ+YVKDGKNYSYKVAG+VA
Sbjct: 481 EES------------GAFGNRA-GSEPSDAP--KWGSRKNPQFYVKDGKNYSYKVAGAVA 540

Query: 748 VANSDEASLVTQAQKELIYGLGRGGNLPLGAVKKLTE--SQQDSQPASAV-VDPRD---- 807
           VAN++EASLV +A+ + I GLGRGGN+PLGAV+KLTE  +Q+D +  S V V+P D    
Sbjct: 541 VANANEASLVNEAEGDKILGLGRGGNIPLGAVRKLTEYQAQRDEKGQSNVNVNPSDGREN 600

Query: 808 --NVRKTWGRGR----RERERERESGAAPGMPEGEGKQPNVAEASDRGGRGGNRGRGRRG 867
             N R   GRGR    RE+ +E+ + ++    E         EA + GGRG  RGRGRRG
Sbjct: 601 GRNWRGVRGRGRGVVNREQPQEKSNESSNNNSEVN------TEAENGGGRG--RGRGRRG 656

Query: 868 GG---DHHRKDRAIRKHFAGLSGF 870
           GG   +H+ KDRA++KH A +SGF
Sbjct: 661 GGGGRNHNHKDRAMKKHIASVSGF 656