IVF0013294 (gene) Melon (IVF77) v1

Overview
NameIVF0013294
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPectinesterase
Locationtig00000656: 236845 .. 239029 (+)
RNA-Seq ExpressionIVF0013294
SyntenyIVF0013294
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACAATACAAGTTTTGGACTTTGATTATTCAAACTCTTATTATTTTCACATAGCCAACCTGTCTCCTCTTCTTCCCCTGTACTATATAATCAAACCTAAGAGATCTATCTTAGCTTCATCAAACCAAGAAATGGCTGGTCTTTCAATGGAGACAAGCCTTGTTGGAGTCTTCCTTTTTCTTCACTTCTTTCTACTATCTTCTTCAGCCTTTTCCAAGCCTGCCTCTAATGGCATTGATTGGTGGTGCAAAAAAACCACATACCCCGAAACCTGCAAGTACTTTTTCAACCATGGCACCAAGTCTCCTCCCAAAGATATGACTGACTTCAAGAAAATGGTGATCCAATTCGCCATGGAAAGAGCTCTGAGTGCCGAGAGCCATACTAAAGGTGTTGGTTCCAAGTGTCGAAATGGAAAGGAAAAGGCTGCTTGGGCTGATTGCTTGAAGCTTTACCAAAATACCATCCTTCAGCTTAACCAAACCCTTGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTTGAGACGTGCCGCACCGGGTTTGCTGAGCTTAATGTTTTGGACTATATATGGCCACTGATCATGTCAGACAATGTCACTAAGTTAATAAGTAACAGTTTGGCTATTAATAATGCTTCAGCTGGGGTGGGGAATGGGAAGGTGACATACAAAAAAGGATTTCCAAGTTGGCTTTCGGGTGGTGATAGGAGGTTACTGCAGTCGGGTTCAACAAAGGTGGACCTTGTGGTGGCTCAGGATGGATCCGGTAACTATACGACGGTAGGAGCTGCTCTTGAGGAAGCTGCAAAAAGGAAGACAAGTGGGAGATTTGTTATACAAGTAAAGAGAGGAGTTTACAGAGAAAATCTCGAGATAGGAAGTAAGATGAAGAATATAATGCTCATTGGAGATGGAATGAGATTTACCTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGGTAAGCCTCTCTCTCTCTCTCTCTCTCTCTAGGTTCATAGGTTGACTGTTTATAAGATAACTAGCCTGCATCATAACAACATTTGAACATGTCAACCTGCATATTTGTCCCATTCCAACTCCACCAAACCTACTGACATGACATGGCAGCTTTGTCATCTAAAATAAAGTTTCTTCTATTTCAAGACACGGTGATTAGACTTTTCTCCTATCCTCCAGTTCCAAATAAAAATTATAAAATATAAGTTTCTCAAAGCTAGAATTATTTGTGGCCAGCATCCCAGCTTTCTTTGTCAACATCAATATAAAAATGGCAACATATATATACCGTATTTCATACTATACATCATATGGCTGCAAAAGTCAAATACTTGTATACGTTTTCACTTTTATTTATACATTCCTAAAACAGCATTGTGAAATCAAATATGAATAACGAGAAATACTTGTTTGTATTTCGTGACAATCAGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCAGAATACCGCTGGTCCGGAGAACCATCAAGCAGTTGCACTCCGATCTGGCGCTGATCTTTCTGTTTTCTACCGTTGTGCCTTTGAAGGGTATCAAGACACCCTTTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACCGTAGATTTTATATTTGGCAATGCTGCAGTTGTTCTACAGAATTGCATGATTTATGCAAGGAAGCCAATGAATGGTCAGAAGAACGCAGTCACTGCTCAAGGACGGACAGATCCAAATCAAAATACTGGCATTTCAATCCACAATTCACGAGTCATGGCCACAGATGACCTAAAACCAGTGGAGAGCACAGTGAAAACCTACTTAGGAAGGCCATGGAAGGAGTACTCTCGAACAGTTTTCATGAAAACTTACATTGATAGTTTGGTTGATCCAACAGGTTGGTTGGAGTGGGATGGTGACTTCGCGTTGAATACCTTATATTATGGAGAGTATAACAACATTGGTCCTGGATCACCAATTAGCCAGCGAGTGAAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCAGAGTTCACCGTCCAAAATTTCATTGCAGGGCAGTCGTGGTTGCCAAACACAGAGGTCCCATTCACTCCTGGACTCTAG

mRNA sequence

ATGGATACAATACAAGTTTTGGACTTTGATTATTCAAACTCTTATTATTTTCACATAGCCAACCTGTCTCCTCTTCTTCCCCTGTACTATATAATCAAACCTAAGAGATCTATCTTAGCTTCATCAAACCAAGAAATGGCTGGTCTTTCAATGGAGACAAGCCTTGTTGGAGTCTTCCTTTTTCTTCACTTCTTTCTACTATCTTCTTCAGCCTTTTCCAAGCCTGCCTCTAATGGCATTGATTGGTGGTGCAAAAAAACCACATACCCCGAAACCTGCAAGTACTTTTTCAACCATGGCACCAAGTCTCCTCCCAAAGATATGACTGACTTCAAGAAAATGGTGATCCAATTCGCCATGGAAAGAGCTCTGAGTGCCGAGAGCCATACTAAAGGTGTTGGTTCCAAGTGTCGAAATGGAAAGGAAAAGGCTGCTTGGGCTGATTGCTTGAAGCTTTACCAAAATACCATCCTTCAGCTTAACCAAACCCTTGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTTGAGACGTGCCGCACCGGGTTTGCTGAGCTTAATGTTTTGGACTATATATGGCCACTGATCATGTCAGACAATGTCACTAAGTTAATAAGTAACAGTTTGGCTATTAATAATGCTTCAGCTGGGGTGGGGAATGGGAAGGTGACATACAAAAAAGGATTTCCAAGTTGGCTTTCGGGTGGTGATAGGAGGTTACTGCAGTCGGGTTCAACAAAGGTGGACCTTGTGGTGGCTCAGGATGGATCCGGTAACTATACGACGGTAGGAGCTGCTCTTGAGGAAGCTGCAAAAAGGAAGACAAGTGGGAGATTTGTTATACAAGTAAAGAGAGGAGTTTACAGAGAAAATCTCGAGATAGGAAGTAAGATGAAGAATATAATGCTCATTGGAGATGGAATGAGATTTACCTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCAGAATACCGCTGGTCCGGAGAACCATCAAGCAGTTGCACTCCGATCTGGCGCTGATCTTTCTGTTTTCTACCGTTGTGCCTTTGAAGGGTATCAAGACACCCTTTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACCGTAGATTTTATATTTGGCAATGCTGCAGTTGTTCTACAGAATTGCATGATTTATGCAAGGAAGCCAATGAATGGTCAGAAGAACGCAGTCACTGCTCAAGGACGGACAGATCCAAATCAAAATACTGGCATTTCAATCCACAATTCACGAGTCATGGCCACAGATGACCTAAAACCAGTGGAGAGCACAGTGAAAACCTACTTAGGAAGGCCATGGAAGGAGTACTCTCGAACAGTTTTCATGAAAACTTACATTGATAGTTTGGTTGATCCAACAGGTTGGTTGGAGTGGGATGGTGACTTCGCGTTGAATACCTTATATTATGGAGAGTATAACAACATTGGTCCTGGATCACCAATTAGCCAGCGAGTGAAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCAGAGTTCACCGTCCAAAATTTCATTGCAGGGCAGTCGTGGTTGCCAAACACAGAGGTCCCATTCACTCCTGGACTCTAG

Coding sequence (CDS)

ATGGATACAATACAAGTTTTGGACTTTGATTATTCAAACTCTTATTATTTTCACATAGCCAACCTGTCTCCTCTTCTTCCCCTGTACTATATAATCAAACCTAAGAGATCTATCTTAGCTTCATCAAACCAAGAAATGGCTGGTCTTTCAATGGAGACAAGCCTTGTTGGAGTCTTCCTTTTTCTTCACTTCTTTCTACTATCTTCTTCAGCCTTTTCCAAGCCTGCCTCTAATGGCATTGATTGGTGGTGCAAAAAAACCACATACCCCGAAACCTGCAAGTACTTTTTCAACCATGGCACCAAGTCTCCTCCCAAAGATATGACTGACTTCAAGAAAATGGTGATCCAATTCGCCATGGAAAGAGCTCTGAGTGCCGAGAGCCATACTAAAGGTGTTGGTTCCAAGTGTCGAAATGGAAAGGAAAAGGCTGCTTGGGCTGATTGCTTGAAGCTTTACCAAAATACCATCCTTCAGCTTAACCAAACCCTTGATTCCTCTACTAAATGTACTGAGTTTGATATCCAAACCTGGCTCAGTACTGCCCTAACCAACCTTGAGACGTGCCGCACCGGGTTTGCTGAGCTTAATGTTTTGGACTATATATGGCCACTGATCATGTCAGACAATGTCACTAAGTTAATAAGTAACAGTTTGGCTATTAATAATGCTTCAGCTGGGGTGGGGAATGGGAAGGTGACATACAAAAAAGGATTTCCAAGTTGGCTTTCGGGTGGTGATAGGAGGTTACTGCAGTCGGGTTCAACAAAGGTGGACCTTGTGGTGGCTCAGGATGGATCCGGTAACTATACGACGGTAGGAGCTGCTCTTGAGGAAGCTGCAAAAAGGAAGACAAGTGGGAGATTTGTTATACAAGTAAAGAGAGGAGTTTACAGAGAAAATCTCGAGATAGGAAGTAAGATGAAGAATATAATGCTCATTGGAGATGGAATGAGATTTACCTTCATTACTGGTAATAGAAGTGTAGGAGGAGGCTCCACCACTTTCAATTCTGCTACTGTTGCGGTGACTGGTGAAGGATTTATAGCTCGCGGCATCACATTCCAGAATACCGCTGGTCCGGAGAACCATCAAGCAGTTGCACTCCGATCTGGCGCTGATCTTTCTGTTTTCTACCGTTGTGCCTTTGAAGGGTATCAAGACACCCTTTATGTCCATTCACAAAGACAATTCTATAAGGAGTGTTATATTTACGGCACCGTAGATTTTATATTTGGCAATGCTGCAGTTGTTCTACAGAATTGCATGATTTATGCAAGGAAGCCAATGAATGGTCAGAAGAACGCAGTCACTGCTCAAGGACGGACAGATCCAAATCAAAATACTGGCATTTCAATCCACAATTCACGAGTCATGGCCACAGATGACCTAAAACCAGTGGAGAGCACAGTGAAAACCTACTTAGGAAGGCCATGGAAGGAGTACTCTCGAACAGTTTTCATGAAAACTTACATTGATAGTTTGGTTGATCCAACAGGTTGGTTGGAGTGGGATGGTGACTTCGCGTTGAATACCTTATATTATGGAGAGTATAACAACATTGGTCCTGGATCACCAATTAGCCAGCGAGTGAAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCAGAGTTCACCGTCCAAAATTTCATTGCAGGGCAGTCGTGGTTGCCAAACACAGAGGTCCCATTCACTCCTGGACTCTAG

Protein sequence

MDTIQVLDFDYSNSYYFHIANLSPLLPLYYIIKPKRSILASSNQEMAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Homology
BLAST of IVF0013294 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 5.7e-178
Identity = 315/511 (61.64%), Postives = 393/511 (76.91%), Query Frame = 0

Query: 62  LHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTK-SPPKDMTDFKKMVIQFAM 121
           L  F LS ++F   +   +  WC KT  P+ C+YF    T  +  K  TDF K+ +Q A+
Sbjct: 11  LVLFSLSHTSFGY-SPEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLAL 70

Query: 122 ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWLS 181
           ERA +A+S T  +GSKCRN +EKAAW DC +LY+ T+L+LNQT +SS  CT+ D QTWLS
Sbjct: 71  ERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLS 130

Query: 182 TALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGFP 241
           TALTNLETCR    +L V +Y+ PL +S+NVTKLISN+L++N     V   + +YK GFP
Sbjct: 131 TALTNLETCRASLEDLGVPEYVLPL-LSNNVTKLISNTLSLNK----VPYNEPSYKDGFP 190

Query: 242 SWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRE 301
           +W+  GDR+LLQ+ + + ++VVAQDGSGN  T+  A+  AA R    R+VI +K G Y E
Sbjct: 191 TWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNE 250

Query: 302 NLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAG 361
           N+E+  K+KNIM +GDG+  T ITG++SVGGG+TTF SATVAV G+ FIAR IT +NTAG
Sbjct: 251 NIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAG 310

Query: 362 PENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQ 421
           P NHQAVALRSG+DLSVFYRC+FEGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQ
Sbjct: 311 PNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQ 370

Query: 422 NCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWK 481
           NC I+ARKP N + N +TAQGRTDPNQ+TGI IHN RV A  DLKPV+S+VKT+LGRPWK
Sbjct: 371 NCNIFARKPPN-RTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWK 430

Query: 482 EYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVIS 541
           +YSRTV++KT++DSL++P GW+EW GDFALNTLYY EY N GPGS  + RVKW+GYHV++
Sbjct: 431 QYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLT 490

Query: 542 NSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           + ++ S+FTV NFIAG SWLP T VPFT GL
Sbjct: 491 SPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of IVF0013294 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 596.3 bits (1536), Expect = 3.7e-169
Identity = 296/512 (57.81%), Postives = 383/512 (74.80%), Query Frame = 0

Query: 61  FLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP-KDMTDFKKMVIQFA 120
           F+   +L +S  S      +  WC +T  P+ C+YF  H + + P K  ++F K+ ++  
Sbjct: 11  FVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLV 70

Query: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWL 180
           ++RA+ A++H   +G KCR+ +EKAAW DC+KLY  T+ ++N+T+D + KC++ D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 181 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGF 240
           STALTNL+TCR GF EL V D + PL MS+NV+ L+ N+LAIN       N     K GF
Sbjct: 131 STALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKVPF---NYTPPEKDGF 190

Query: 241 PSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300
           PSW+  GDR+LLQS + K + VVA+DGSGN+ T+  A++ A+    SGRFVI VK+GVY 
Sbjct: 191 PSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYS 250

Query: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 360
           ENLEI  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GFIARGITF+NTA
Sbjct: 251 ENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTA 310

Query: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420
           G  N QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VL
Sbjct: 311 GASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVL 370

Query: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480
           QNC I+AR+P   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW
Sbjct: 371 QNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 430

Query: 481 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540
           ++YSRTVFMKT +DSL+DP GWLEWDG+FAL TL+Y E+ N GPG+  S RV W G+ V+
Sbjct: 431 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 490

Query: 541 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
            +++EAS+FTV  F+AG SW+P++ VPFT GL
Sbjct: 491 GSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of IVF0013294 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 292/528 (55.30%), Postives = 375/528 (71.02%), Query Frame = 0

Query: 60  LFLHFF---LLSSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDFKKMV 119
           LFLH     LL     +  A      WC KT YP+ CK YF NH     P  +++F+ M+
Sbjct: 11  LFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVML 70

Query: 120 IQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSST 179
           ++ AM+RA+SA +     G  C + K++A  ADC+ LY +TI+QLN+TL        ++ 
Sbjct: 71  VEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAK 130

Query: 180 KCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGV 239
            CT+FD QTWLSTALTN ETCR G ++LNV D+I P++ +  ++ LISN LA+N A    
Sbjct: 131 SCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTA 190

Query: 240 GN-GKVT-YKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRK-T 299
           GN G  T  +KGFP+WLS  D+RLL+  + + +LVVA+DGSG++ TV AA++ A +RK T
Sbjct: 191 GNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVT 250

Query: 300 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 359
           SGRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV GG TT+NSAT  + G
Sbjct: 251 SGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 310

Query: 360 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 419
             FIA+GITF+NTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 311 LHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIY 370

Query: 420 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 479
           GTVDFIFGNAA V QNC+I  R+P+ GQ N +TAQGR DP QNTGISIHNSR++   DLK
Sbjct: 371 GTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLK 430

Query: 480 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW-DGD-FALNTLYYGEYNNIGP 539
           PV  TVKTY+GRPW ++SRTV ++TY+D++V P GW  W +G  F L+TL+Y EY N GP
Sbjct: 431 PVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGP 490

Query: 540 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
            S    RV WKG+HV+  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 491 ASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of IVF0013294 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 568.5 bits (1464), Expect = 8.3e-161
Identity = 289/530 (54.53%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 59  FLFLHFFLL------SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDF 118
           FL LH  L+        +  +   S  ID WC KT YP  CK YF  H     P  +++F
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 119 KKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD-----S 178
           + ++++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN+TL      +
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 179 STKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNA-- 238
             +CT+FD QTWLSTALTN ETCR G ++LNV D+  P++ +  ++ LISN LA+N A  
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 239 SAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRK 298
           +AG  +      KGFP+W+S  +RRLLQ  S + +LVVA+DGSG++ TV AA++ A +RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 299 -TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 358
            TSGRFVI VKRG+Y+ENL +     NIML+GDGMR+T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 359 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 418
            G  FIA+GI FQNTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 419 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 478
           IYGTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   D
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 479 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNI 538
           LKPV  +VKTY+GRPW  YSRTV +KTYIDS+V P GW  W     + L+TL+Y EY NI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 539 GPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           GP S    RV+WKG+HV+S +++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of IVF0013294 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 1.8e-155
Identity = 276/525 (52.57%), Postives = 362/525 (68.95%), Query Frame = 0

Query: 71  AFSKPASNG-----IDWWCKKTTYPETCKYFFNHGTKS-----PPKDMTDFKKMVIQFAM 130
           A   P +NG     + WWC KT + ETC Y+F   +++     PP+  ++F +M+++ A+
Sbjct: 70  AAPSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVAL 129

Query: 131 ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSSTKCTEF 190
           ++A+   S T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S  KCT+F
Sbjct: 130 DQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDF 189

Query: 191 DIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKV 250
           D QTWLSTA TN+ETCR+G  +LNV D++ P+I + N++ LI N LA+N       +   
Sbjct: 190 DAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTT 249

Query: 251 T--YKKGFPSWLSGGDRRLLQSGS---TKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGR 310
           T  +K+ FPSW+S  +RRLL S S   +   LVVAQD SG++ ++ AA+  AA+R+   R
Sbjct: 250 TANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSR 309

Query: 311 FVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF 370
           FVI VK+GVYREN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F
Sbjct: 310 FVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRF 369

Query: 371 IARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTV 430
           +A+ +TF NTAGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+
Sbjct: 370 VAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTI 429

Query: 431 DFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVE 490
           DFIFGNAAVV QNCMI  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV 
Sbjct: 430 DFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVI 489

Query: 491 STVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNIGPGSP 550
              KTYLGRPW+ YSR   MKTYID+ + P GW  W    +FALNT++YGEY N GPGS 
Sbjct: 490 RAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSS 549

Query: 551 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
              RV+WKG+H I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 550 TRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of IVF0013294 vs. ExPASy TrEMBL
Match: A0A0A0L9G4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1)

HSP 1 Score: 1102.4 bits (2850), Expect = 0.0e+00
Identity = 551/572 (96.33%), Postives = 556/572 (97.20%), Query Frame = 0

Query: 1   MDTIQVLDFDYSNSYYFHIANLSPLLPLYYIIKPKRSILASSNQEMAGLSMETSLVGVFL 60
           MDTIQVLDFDYSN  YF IANLSPLLPLYYIIK KRSILASSN EMAGLSM TS  GVF+
Sbjct: 1   MDTIQVLDFDYSNCCYFQIANLSPLLPLYYIIKHKRSILASSNIEMAGLSMGTSF-GVFI 60

Query: 61  FLHFFLL-SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA 120
           FLHFFLL SSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA
Sbjct: 61  FLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA 120

Query: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWL 180
           MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTK TEFDIQTWL
Sbjct: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWL 180

Query: 181 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGF 240
           STALTNLETCRTGFAELNV DYI PLIMSDNVT+LISNSLAINNASAGVGNGK TYKKGF
Sbjct: 181 STALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNASAGVGNGKETYKKGF 240

Query: 241 PSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300
           PSWLSGGDRRLLQS STKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR
Sbjct: 241 PSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300

Query: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 360
           ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITF+NTA
Sbjct: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTA 360

Query: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420
           GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL
Sbjct: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420

Query: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480
           QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW
Sbjct: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480

Query: 481 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540
           KEYSRTVFMKTYIDSLVDP GWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI
Sbjct: 481 KEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540

Query: 541 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           +N TEASEFTVQNFIAGQSWLP+TEVPFTPGL
Sbjct: 541 TNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

BLAST of IVF0013294 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 46  MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105
           MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165
           KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 166 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 225
           SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS
Sbjct: 121 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 226 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285
           AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT
Sbjct: 181 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345
           SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 346 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 405
           EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 360

Query: 406 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 465
           GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK
Sbjct: 361 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 420

Query: 466 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 525
           PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS
Sbjct: 421 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 480

Query: 526 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Sbjct: 481 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

BLAST of IVF0013294 vs. ExPASy TrEMBL
Match: A0A1S3AYJ9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1)

HSP 1 Score: 1061.6 bits (2744), Expect = 1.2e-306
Identity = 523/526 (99.43%), Postives = 523/526 (99.43%), Query Frame = 0

Query: 46  MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105
           MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165
           KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 166 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 225
           SSTK TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS
Sbjct: 121 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 226 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285
           A VGNGKVTYKKGFPSWLSGGDRRLLQS STKVDLVVAQDGSGNYTTVGAALEEAAKRKT
Sbjct: 181 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345
           SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 346 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 405
           EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 360

Query: 406 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 465
           GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK
Sbjct: 361 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 420

Query: 466 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 525
           PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS
Sbjct: 421 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 480

Query: 526 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Sbjct: 481 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

BLAST of IVF0013294 vs. ExPASy TrEMBL
Match: A0A6J1CH86 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 9.9e-266
Identity = 464/554 (83.75%), Postives = 495/554 (89.35%), Query Frame = 0

Query: 22  LSPLLP----LYYIIKPKRSILASSNQEMAGLSMETSLVGVFLFLHFFLLSSSAFSKPAS 81
           +SP  P    L YI KP+ + LASS QE A L ME S  GV  FL FF LSS AFSK AS
Sbjct: 8   VSPFHPNLALLPYITKPEVACLASSKQEPA-LLMEKSF-GVLFFLLFFSLSSPAFSKHAS 67

Query: 82  NGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKC 141
           NGIDWWC KTTYPETCKYFFNHG+ + PKDM++FKKMV +FAMERAL AESH KGVGSKC
Sbjct: 68  NGIDWWCSKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKC 127

Query: 142 RNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWLSTALTNLETCRTGFAELN 201
           RN KEKAAWADCL+LYQNTILQLN TLDSS+KCTEFDIQTWLSTALTNLETC TGFAE N
Sbjct: 128 RNEKEKAAWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFN 187

Query: 202 VLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTK 261
           V D++ PLIMS+NVTKLISNSLAINNASA    GK TYK+GFPSWL GGDRRLLQS S K
Sbjct: 188 VSDHVLPLIMSNNVTKLISNSLAINNASA--WPGKETYKEGFPSWLLGGDRRLLQSSSVK 247

Query: 262 VDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDG 321
            DLVVAQDGSG++ TVGAALE AAKRKT GRFVI VKRGVY ENLEIG+KMKNIML+GDG
Sbjct: 248 TDLVVAQDGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDG 307

Query: 322 MRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAGPENHQAVALRSGADLSV 381
           MRFT ITG+RSV GGSTTF+SATVAVTGEGFIARGITF+NTAGPENHQAVALRSGAD SV
Sbjct: 308 MRFTIITGSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSV 367

Query: 382 FYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAV 441
           FYRC FEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNAV
Sbjct: 368 FYRCGFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAV 427

Query: 442 TAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVD 501
           TAQGRTDPNQNTGISIHNSRVMATDDLK VESTVKTYLGRPWKEYSRTVFM+TY+DSLVD
Sbjct: 428 TAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVD 487

Query: 502 PTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQ 561
           P GWLEWDG+FALNTLYYGEY N+GPGS  SQRVKW+GYH+++NSTEAS+FTVQNFIAGQ
Sbjct: 488 PAGWLEWDGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQ 547

Query: 562 SWLPNTEVPFTPGL 572
           SWLP TEVPFTPGL
Sbjct: 548 SWLPGTEVPFTPGL 557

BLAST of IVF0013294 vs. ExPASy TrEMBL
Match: A0A6J1JEY1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.1e-259
Identity = 449/548 (81.93%), Postives = 484/548 (88.32%), Query Frame = 0

Query: 25  LLPLYYIIKPKRSILASSN-QEMAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWW 84
           LLP  YI KP R+ LA  N     GLSME S++GV  FLHFFL SSSAFSK ASNGI WW
Sbjct: 7   LLP--YITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWW 66

Query: 85  CKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEK 144
           C KTTYPETCKYF+ +    PPKDM++FKKM I+FAM+ ALS E HTK VGS+CRN KE+
Sbjct: 67  CTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKER 126

Query: 145 AAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIW 204
            AWADCLKLYQNTI QL +TL+SSTK TEFDIQTWLSTALTN+ETCRTGFAELNV+D + 
Sbjct: 127 VAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVL 186

Query: 205 PLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVA 264
           PLI S NVTKLISNSLA+NNAS     GK TYK+GFPSWLSGGDR+LLQS S  VDLVVA
Sbjct: 187 PLITSKNVTKLISNSLAVNNAS--TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVA 246

Query: 265 QDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFI 324
           QDGSG++ TV AALEEAAKR T GRFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTFI
Sbjct: 247 QDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFI 306

Query: 325 TGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAF 384
           T +RSVGGGSTTFNSATVAVTGEGFIA+GITF+NTAGPENHQAVALRSGAD SVFYRCAF
Sbjct: 307 TSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAF 366

Query: 385 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRT 444
           EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGRT
Sbjct: 367 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRT 426

Query: 445 DPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLE 504
           DPNQNTGISIHNSRVMATDDLK VE TVKT+LGRPWKEYSRTVF+KTY+DSLVDP GWLE
Sbjct: 427 DPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLE 486

Query: 505 WDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNT 564
           W+GDFALNTLYYGEY NIGP S   QRV+WKGYHVISNSTEASEF+VQNFI+GQSWLP T
Sbjct: 487 WNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGT 546

Query: 565 EVPFTPGL 572
           EVPF PG+
Sbjct: 547 EVPFIPGI 550

BLAST of IVF0013294 vs. NCBI nr
Match: XP_004148139.2 (pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [Cucumis sativus])

HSP 1 Score: 1096 bits (2834), Expect = 0.0
Identity = 551/572 (96.33%), Postives = 556/572 (97.20%), Query Frame = 0

Query: 1   MDTIQVLDFDYSNSYYFHIANLSPLLPLYYIIKPKRSILASSNQEMAGLSMETSLVGVFL 60
           MDTIQVLDFDYSN  YF IANLSPLLPLYYIIK KRSILASSN EMAGLSM TS  GVF+
Sbjct: 1   MDTIQVLDFDYSNCCYFQIANLSPLLPLYYIIKHKRSILASSNIEMAGLSMGTSF-GVFI 60

Query: 61  FLHFFLLSSS-AFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA 120
           FLHFFLLSSS AFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA
Sbjct: 61  FLHFFLLSSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFA 120

Query: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWL 180
           MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTK TEFDIQTWL
Sbjct: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKSTEFDIQTWL 180

Query: 181 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGF 240
           STALTNLETCRTGFAELNV DYI PLIMSDNVT+LISNSLAINNASAGVGNGK TYKKGF
Sbjct: 181 STALTNLETCRTGFAELNVSDYILPLIMSDNVTELISNSLAINNASAGVGNGKETYKKGF 240

Query: 241 PSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300
           PSWLSGGDRRLLQS STKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR
Sbjct: 241 PSWLSGGDRRLLQSSSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300

Query: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 360
           ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITF+NTA
Sbjct: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFRNTA 360

Query: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420
           GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL
Sbjct: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420

Query: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480
           QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW
Sbjct: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480

Query: 481 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540
           KEYSRTVFMKTYIDSLVDP GWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI
Sbjct: 481 KEYSRTVFMKTYIDSLVDPAGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540

Query: 541 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 571
           +N TEASEFTVQNFIAGQSWLP+TEVPFTPGL
Sbjct: 541 TNLTEASEFTVQNFIAGQSWLPDTEVPFTPGL 571

BLAST of IVF0013294 vs. NCBI nr
Match: KAA0067674.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1065 bits (2754), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 46  MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105
           MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165
           KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 166 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 225
           SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS
Sbjct: 121 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 226 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285
           AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT
Sbjct: 181 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345
           SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 346 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 405
           EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 360

Query: 406 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 465
           GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK
Sbjct: 361 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 420

Query: 466 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 525
           PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS
Sbjct: 421 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 480

Query: 526 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 571
           PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Sbjct: 481 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

BLAST of IVF0013294 vs. NCBI nr
Match: XP_008439095.2 (PREDICTED: pectinesterase-like [Cucumis melo])

HSP 1 Score: 1056 bits (2730), Expect = 0.0
Identity = 523/526 (99.43%), Postives = 523/526 (99.43%), Query Frame = 0

Query: 46  MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 105
           MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP
Sbjct: 1   MAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP 60

Query: 106 KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 165
           KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD
Sbjct: 61  KDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD 120

Query: 166 SSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 225
           SSTK TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS
Sbjct: 121 SSTKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNAS 180

Query: 226 AGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 285
           A VGNGKVTYKKGFPSWLSGGDRRLLQS STKVDLVVAQDGSGNYTTVGAALEEAAKRKT
Sbjct: 181 AAVGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKT 240

Query: 286 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 345
           SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG
Sbjct: 241 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 300

Query: 346 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 405
           EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY
Sbjct: 301 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 360

Query: 406 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 465
           GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK
Sbjct: 361 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 420

Query: 466 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 525
           PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS
Sbjct: 421 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGS 480

Query: 526 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 571
           PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL
Sbjct: 481 PISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

BLAST of IVF0013294 vs. NCBI nr
Match: XP_038882414.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 975 bits (2520), Expect = 0.0
Identity = 485/521 (93.09%), Postives = 501/521 (96.16%), Query Frame = 0

Query: 51  METSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKDMTD 110
           ME SL GVF FLHFFLLSS+AFSK ASNGIDWWC+KTTYPETCKYFFN G+K+ PKDMT+
Sbjct: 1   MERSL-GVFFFLHFFLLSSAAFSKHASNGIDWWCRKTTYPETCKYFFNQGSKAAPKDMTE 60

Query: 111 FKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKC 170
           FKKMVIQFAMERAL AESHTKGVGSKCRNGKEKAAWADCLKLYQNTI QLNQTLDS+TKC
Sbjct: 61  FKKMVIQFAMERALGAESHTKGVGSKCRNGKEKAAWADCLKLYQNTIFQLNQTLDSATKC 120

Query: 171 TEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGN 230
           TEFDIQTWLSTALTNLETCRTGFAELNV DYI  LIMS+NVTKLISNSLAINNASAG+G 
Sbjct: 121 TEFDIQTWLSTALTNLETCRTGFAELNVSDYILQLIMSNNVTKLISNSLAINNASAGLG- 180

Query: 231 GKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFV 290
            K TYKKGFPSWLSGGDRRLLQS STKVDLVVAQDGSGN+TTVGAALEEAAKRKT+GRFV
Sbjct: 181 -KETYKKGFPSWLSGGDRRLLQSSSTKVDLVVAQDGSGNHTTVGAALEEAAKRKTNGRFV 240

Query: 291 IQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 350
           IQVKRGVY+ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA
Sbjct: 241 IQVKRGVYQENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIA 300

Query: 351 RGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 410
           RGITF+NTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF
Sbjct: 301 RGITFRNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDF 360

Query: 411 IFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 470
           IFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST
Sbjct: 361 IFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVEST 420

Query: 471 VKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQR 530
           VKTYLGRPWKEYSRTVFMKTY+DSLVDPTGWLEWDGDFAL+TLYYGEYNNIGP SP SQR
Sbjct: 421 VKTYLGRPWKEYSRTVFMKTYLDSLVDPTGWLEWDGDFALDTLYYGEYNNIGPRSPTSQR 480

Query: 531 VKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 571
           V WKGY VI NSTEASEFTVQNFIAGQSWLP+TEVPFTPGL
Sbjct: 481 VNWKGYRVIRNSTEASEFTVQNFIAGQSWLPDTEVPFTPGL 518

BLAST of IVF0013294 vs. NCBI nr
Match: XP_022141175.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 920 bits (2378), Expect = 0.0
Identity = 463/547 (84.64%), Postives = 493/547 (90.13%), Query Frame = 0

Query: 25  LLPLYYIIKPKRSILASSNQEMAGLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWC 84
           LLP  YI KP+ + LASS QE A L ME S  GV  FL FF LSS AFSK ASNGIDWWC
Sbjct: 17  LLP--YITKPEVACLASSKQEPA-LLMEKSF-GVLFFLLFFSLSSPAFSKHASNGIDWWC 76

Query: 85  KKTTYPETCKYFFNHGTKSPPKDMTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKA 144
            KTTYPETCKYFFNHG+ + PKDM++FKKMV +FAMERAL AESH KGVGSKCRN KEKA
Sbjct: 77  SKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKA 136

Query: 145 AWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWP 204
           AWADCL+LYQNTILQLN TLDSS+KCTEFDIQTWLSTALTNLETC TGFAE NV D++ P
Sbjct: 137 AWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLP 196

Query: 205 LIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQ 264
           LIMS+NVTKLISNSLAINNASA  G  K TYK+GFPSWL GGDRRLLQS S K DLVVAQ
Sbjct: 197 LIMSNNVTKLISNSLAINNASAWPG--KETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQ 256

Query: 265 DGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFIT 324
           DGSG++ TVGAALE AAKRKT GRFVI VKRGVY ENLEIG+KMKNIML+GDGMRFT IT
Sbjct: 257 DGSGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIIT 316

Query: 325 GNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFE 384
           G+RSV GGSTTF+SATVAVTGEGFIARGITF+NTAGPENHQAVALRSGAD SVFYRC FE
Sbjct: 317 GSRSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFE 376

Query: 385 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTD 444
           GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNAVTAQGRTD
Sbjct: 377 GYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTD 436

Query: 445 PNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW 504
           PNQNTGISIHNSRVMATDDLK VESTVKTYLGRPWKEYSRTVFM+TY+DSLVDP GWLEW
Sbjct: 437 PNQNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEW 496

Query: 505 DGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTE 564
           DG+FALNTLYYGEY N+GPGS  SQRVKW+GYH+++NSTEAS+FTVQNFIAGQSWLP TE
Sbjct: 497 DGEFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTE 556

Query: 565 VPFTPGL 571
           VPFTPGL
Sbjct: 557 VPFTPGL 557

BLAST of IVF0013294 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 596.3 bits (1536), Expect = 2.6e-170
Identity = 296/512 (57.81%), Postives = 383/512 (74.80%), Query Frame = 0

Query: 61  FLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPP-KDMTDFKKMVIQFA 120
           F+   +L +S  S      +  WC +T  P+ C+YF  H + + P K  ++F K+ ++  
Sbjct: 11  FVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLV 70

Query: 121 MERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSSTKCTEFDIQTWL 180
           ++RA+ A++H   +G KCR+ +EKAAW DC+KLY  T+ ++N+T+D + KC++ D QTWL
Sbjct: 71  LDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWL 130

Query: 181 STALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKVTYKKGF 240
           STALTNL+TCR GF EL V D + PL MS+NV+ L+ N+LAIN       N     K GF
Sbjct: 131 STALTNLDTCRAGFLELGVTDIVLPL-MSNNVSNLLCNTLAINKVPF---NYTPPEKDGF 190

Query: 241 PSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGRFVIQVKRGVYR 300
           PSW+  GDR+LLQS + K + VVA+DGSGN+ T+  A++ A+    SGRFVI VK+GVY 
Sbjct: 191 PSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAAS---GSGRFVIYVKQGVYS 250

Query: 301 ENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGFIARGITFQNTA 360
           ENLEI  + KN+ML GDG+  T ITG++SVGGG+TTFNSATVA  G+GFIARGITF+NTA
Sbjct: 251 ENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTA 310

Query: 361 GPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 420
           G  N QAVALRSG+DLSVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA VL
Sbjct: 311 GASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVL 370

Query: 421 QNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVESTVKTYLGRPW 480
           QNC I+AR+P   + N +TAQGR+DPNQNTGI IHNSRV A  DL+PV  + KTYLGRPW
Sbjct: 371 QNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPW 430

Query: 481 KEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPISQRVKWKGYHVI 540
           ++YSRTVFMKT +DSL+DP GWLEWDG+FAL TL+Y E+ N GPG+  S RV W G+ V+
Sbjct: 431 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 490

Query: 541 SNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
            +++EAS+FTV  F+AG SW+P++ VPFT GL
Sbjct: 491 GSASEASKFTVGTFLAGGSWIPSS-VPFTSGL 511

BLAST of IVF0013294 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 577.4 bits (1487), Expect = 1.3e-164
Identity = 292/528 (55.30%), Postives = 375/528 (71.02%), Query Frame = 0

Query: 60  LFLHFF---LLSSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDFKKMV 119
           LFLH     LL     +  A      WC KT YP+ CK YF NH     P  +++F+ M+
Sbjct: 11  LFLHLILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQLSEFRVML 70

Query: 120 IQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSST 179
           ++ AM+RA+SA +     G  C + K++A  ADC+ LY +TI+QLN+TL        ++ 
Sbjct: 71  VEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAK 130

Query: 180 KCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGV 239
            CT+FD QTWLSTALTN ETCR G ++LNV D+I P++ +  ++ LISN LA+N A    
Sbjct: 131 SCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTA 190

Query: 240 GN-GKVT-YKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRK-T 299
           GN G  T  +KGFP+WLS  D+RLL+  + + +LVVA+DGSG++ TV AA++ A +RK T
Sbjct: 191 GNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVT 250

Query: 300 SGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTG 359
           SGRFVI VKRG+Y+EN+ +     +IML+GDGMR T ITG RSV GG TT+NSAT  + G
Sbjct: 251 SGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGIEG 310

Query: 360 EGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 419
             FIA+GITF+NTAGP   QAVALRS +DLS+FY+C+ EGYQDTL VHSQRQFY+ECYIY
Sbjct: 311 LHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYRECYIY 370

Query: 420 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLK 479
           GTVDFIFGNAA V QNC+I  R+P+ GQ N +TAQGR DP QNTGISIHNSR++   DLK
Sbjct: 371 GTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILPAPDLK 430

Query: 480 PVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW-DGD-FALNTLYYGEYNNIGP 539
           PV  TVKTY+GRPW ++SRTV ++TY+D++V P GW  W +G  F L+TL+Y EY N GP
Sbjct: 431 PVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEYKNTGP 490

Query: 540 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
            S    RV WKG+HV+  +++AS FTV  FIAG +WLP T +PFT GL
Sbjct: 491 ASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of IVF0013294 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 568.5 bits (1464), Expect = 5.9e-162
Identity = 289/530 (54.53%), Postives = 370/530 (69.81%), Query Frame = 0

Query: 59  FLFLHFFLL------SSSAFSKPASNGIDWWCKKTTYPETCK-YFFNHGTKSPPKDMTDF 118
           FL LH  L+        +  +   S  ID WC KT YP  CK YF  H     P  +++F
Sbjct: 11  FLSLHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 70

Query: 119 KKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLD-----S 178
           + ++++ AM+RA+SA          C + K++A  ADC+ LY +T++QLN+TL      +
Sbjct: 71  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 130

Query: 179 STKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNA-- 238
             +CT+FD QTWLSTALTN ETCR G ++LNV D+  P++ +  ++ LISN LA+N A  
Sbjct: 131 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALL 190

Query: 239 SAGVGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRK 298
           +AG  +      KGFP+W+S  +RRLLQ  S + +LVVA+DGSG++ TV AA++ A +RK
Sbjct: 191 TAGKNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRK 250

Query: 299 -TSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 358
            TSGRFVI VKRG+Y+ENL +     NIML+GDGMR+T ITG RSV GG TT++SAT  +
Sbjct: 251 VTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

Query: 359 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 418
            G  FIA+GI FQNTAGP   QAVALRS +DLS+FYRC+ EGYQDTL VHSQRQFY+ECY
Sbjct: 311 EGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECY 370

Query: 419 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 478
           IYGTVDFIFGNAAVV QNC+I  R P+ GQ N +TAQGRTD  QNTGISIHNS ++   D
Sbjct: 371 IYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPD 430

Query: 479 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNI 538
           LKPV  +VKTY+GRPW  YSRTV +KTYIDS+V P GW  W     + L+TL+Y EY NI
Sbjct: 431 LKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNI 490

Query: 539 GPGSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           GP S    RV+WKG+HV+S +++AS F+V  FIAG +WLP + +PFT  L
Sbjct: 491 GPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of IVF0013294 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 550.8 bits (1418), Expect = 1.3e-156
Identity = 276/525 (52.57%), Postives = 362/525 (68.95%), Query Frame = 0

Query: 71  AFSKPASNG-----IDWWCKKTTYPETCKYFFNHGTKS-----PPKDMTDFKKMVIQFAM 130
           A   P +NG     + WWC KT + ETC Y+F   +++     PP+  ++F +M+++ A+
Sbjct: 70  AAPSPINNGSVSGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVAL 129

Query: 131 ERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTL-------DSSTKCTEF 190
           ++A+   S T   G  C N + KAAW+DC+ L+QNT+ QLN+TL        S  KCT+F
Sbjct: 130 DQAVITHSQTVKFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDF 189

Query: 191 DIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAGVGNGKV 250
           D QTWLSTA TN+ETCR+G  +LNV D++ P+I + N++ LI N LA+N       +   
Sbjct: 190 DAQTWLSTAQTNIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTT 249

Query: 251 T--YKKGFPSWLSGGDRRLLQSGS---TKVDLVVAQDGSGNYTTVGAALEEAAKRKTSGR 310
           T  +K+ FPSW+S  +RRLL S S   +   LVVAQD SG++ ++ AA+  AA+R+   R
Sbjct: 250 TANHKEYFPSWVSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSR 309

Query: 311 FVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEGF 370
           FVI VK+GVYREN+++G+   NIML+GDG R T IT  RSV  G TT+NSAT    G+ F
Sbjct: 310 FVIYVKKGVYRENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRF 369

Query: 371 IARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTV 430
           +A+ +TF NTAGP   QAVA+RS +DLSVFYR    G+QDTLY+HSQRQF++ECYI GT+
Sbjct: 370 VAKDMTFINTAGPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTI 429

Query: 431 DFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPVE 490
           DFIFGNAAVV QNCMI  R+P++GQ N +TAQGR DP QNTGI+IH+SR++A  DLKPV 
Sbjct: 430 DFIFGNAAVVFQNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVI 489

Query: 491 STVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEW--DGDFALNTLYYGEYNNIGPGSP 550
              KTYLGRPW+ YSR   MKTYID+ + P GW  W    +FALNT++YGEY N GPGS 
Sbjct: 490 RAYKTYLGRPWQAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSS 549

Query: 551 ISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
              RV+WKG+H I+++  AS FTV + IAG SWLP T VPF  GL
Sbjct: 550 TRWRVRWKGFHAITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of IVF0013294 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 453.8 bits (1166), Expect = 2.1e-127
Identity = 253/528 (47.92%), Postives = 327/528 (61.93%), Query Frame = 0

Query: 84  CKKTTYPETCKYFFNHGTKSPPKDMTD---FKKMVIQFAMERALSAESHTKGVGSKCRNG 143
           CK+T YP  C +  ++       D TD   F  +V+   M++A+  + H      K  + 
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAV--QLHRLVSSLKQHHS 97

Query: 144 KEK---AAWADCLKLYQNTILQLNQTLDS-STKCTEFDIQTWLSTALTNLETCRTGFAEL 203
             K   +A  DCL+LY++TI QLN +  S     +  D QT LS A+ N +TCR GF + 
Sbjct: 98  LHKHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDF 157

Query: 204 NV---LDYIWPLIMSDNVTKLISNSLAINNA------------------------SAGVG 263
            +       +P+    N+TK ISNSLA+  A                        SAG G
Sbjct: 158 KLTSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGG 217

Query: 264 NGK---VTYKKGFPSWLSGGDRRLLQSGST--KVDLVVAQDGSGNYTTVGAALEEAAK-R 323
           + +   +   + FPSW    DR+LL+   T  K DLVVA+DGSG+YT++  A+  AAK  
Sbjct: 218 SHRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLP 277

Query: 324 KTSGRFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAV 383
           + + R VI VK GVYREN+ I   +KN+M+IGDG+  T +TGNR+V  G+TTF SAT AV
Sbjct: 278 RRNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAV 337

Query: 384 TGEGFIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECY 443
           +G GFIA+GITF+NTAGPE HQAVALRS +D SVFY C+F+GYQDTLY+HS RQF + C 
Sbjct: 338 SGNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCN 397

Query: 444 IYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDD 503
           IYGTVDFIFG+A  +LQNC IYARKPM+GQKN +TAQ R +P++ TG  I +S V    +
Sbjct: 398 IYGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE 457

Query: 504 LKPVESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGP 563
                    TYLGRPW+ +SRTVFMK  + +LV P GWL W G FAL+TLYYGEY N G 
Sbjct: 458 ---------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGA 517

Query: 564 GSPISQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 572
           G+ +S RVKW GYHVI   TEA +FTV+NF+ G  W+  T VP   GL
Sbjct: 518 GASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048875.7e-17861.64Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221493.7e-16957.81Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN51.8e-16355.30Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN48.3e-16154.53Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY31.8e-15552.57Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A0A0L9G40.0e+0096.33Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G175720 PE=3 SV=1[more]
A0A5D3DJL71.9e-309100.00Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440... [more]
A0A1S3AYJ91.2e-30699.43Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1[more]
A0A6J1CH869.9e-26683.75Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1[more]
A0A6J1JEY13.1e-25981.93Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004148139.20.096.33pectinesterase 2 [Cucumis sativus] >KGN57272.1 hypothetical protein Csa_010597 [... [more]
KAA0067674.10.0100.00pectinesterase-like [Cucumis melo var. makuwa] >TYK23678.1 pectinesterase-like [... [more]
XP_008439095.20.099.43PREDICTED: pectinesterase-like [Cucumis melo][more]
XP_038882414.10.093.09pectinesterase-like [Benincasa hispida][more]
XP_022141175.10.084.64pectinesterase-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT2G45220.12.6e-17057.81Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.11.3e-16455.30Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.15.9e-16254.53Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.11.3e-15652.57Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G23200.12.1e-12747.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 75..221
e-value: 8.8E-32
score: 121.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 80..221
e-value: 2.9E-26
score: 92.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 54..223
e-value: 1.9E-28
score: 97.0
IPR006633Carbohydrate-binding/sugar hydrolysis domainSMARTSM00722completecoord: 293..459
e-value: 0.0084
score: 25.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 253..571
e-value: 4.8E-126
score: 422.4
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 259..556
e-value: 2.8E-147
score: 489.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 76..224
e-value: 3.8E-30
score: 106.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 78..223
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 56..570
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 56..570
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 82..224
e-value: 8.35951E-35
score: 126.784
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 404..413
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 257..570

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0013294.2IVF0013294.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity