IVF0013022 (gene) Melon (IVF77) v1

Overview
NameIVF0013022
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionSpoU_sub_bind domain-containing protein
Locationchr02: 1371055 .. 1373474 (-)
RNA-Seq ExpressionIVF0013022
SyntenyIVF0013022
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTATCACCATCCTTCTCCATTTCTCAGTCTCTTGCCTCAACCATATCTAGCCACCGTTGAATTTCATCTCGAAACTGTGCGGCGGCGGACATTCGGCGATCAATTAACCGGAGATAAGGTATTCACCGCTCATTTTACAACTCTTGCATTTCAATATTTGTCTTTCTTCTACAGAAATCATGGTTTTTTGCTTACAAATTAAGCTCGTAACTATGGTAAACTTCTAGAATTGTATTCATTCAATGTCGCTGTTTCTTTAACAGAGACGCGCGTTTCTTCACACCCATGAGAGTTTATTCGGGACTAGATGCTCCAAATCATTTACTTCCTCAAATCTCCAAATTTTACATACAGGGCCATCTCCAAAACCCCCAATAAACCCTAGTTTGCTCACTACATTTTCCTCATCTAGGTCATTTTCAAGCTTTCACATTAATCTCTCTATGGCTAACCTTGTGAAGCTTTCAATTCGTGGTCGCCATTGTTTTCCAGAAATTAGGGATTTAGTAAGCCCCCAATTGAACTCGCTGCCTTTGAGCGTTGGGAGTTCTTTGAAATCTTTGTCCTTGAATAAATCTCGATGTTTTTCGAGTTCCTATCGTCTTAAGGACGAAGGAGTGTTATCTGGGAATAAGCTTCCTTGGTTAGCTGCAGCTAACAGTGTTGAGAAGGCAAAATGGTTGGATAAATCTACTACAAAGAAGAGAGGGGACCGATCGTCTTGGAAGGAATCGGATTTTAAGTCAAAGAGATTTGGAAAAAACGGCAATAGGTCGTCTCGGGATGAACCGGAAAAGAGATATGGCAAAGAACCAATTGGTAGTGCTAATCGTTCGACTTGGGAGGTCTCTGCTGAGAAATTTGCTAGACGAGGTATTGAGTCAGCAGATGAAAGAAGAGAAAGGAGGACAAGAATGGACAATGAAGAGCAGGAAAACGATGATATCGAAAGGAGGGGAAATTATGAAGAAGGGGAAGATGAAGAAATAGAAGCCATTGCAGACCCTAGATGGGATAAGATTAAAAGTAGATTTAGGGGAATGGTGGATGAAAGAGGTGCAACTGAGCGGCCTCAGTTTCAGAGATGGAATAAACAAGAAAGTTGGGGCAGAAAAACATGGAAGGAAGCTTCTGAATCCACTTTACCTAAAATGATTGGTGAAGGGGTTTATGGCGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGGATTGACTTGAACAATAACAATAAGAAGAAGAAGGACAAGAAAGGGTTTGAGAAAGTTCTCAAGTTGGCTGAAAAGAATGCATTAACTATCAAGGAAATATCGAAGCACGATCTCAATATGATTTCTGATAACCGACCTCATCAAGGTCTTGTGTTAGATACTTCTCCCTTGGAGATGGTTAAAATAAAAGAATTGGATCCTGTCTCACTCGAAGATGACAAAGGTTCACTTTGGTTGGCTTTGGATGAAGTTACTGACCCACAGAACTTGGGGGCAATAATAAGATCAGCTTATTTCTTTGGCGCATCGGGAGTTGTTTTATGTGCAAAAAATTCAGCTCCATTAAGTGGTGTGGTTAGTAAAGCAAGTGCTGGTTCACTTGAGTTAATGGAGCTTAGATACTGTAAGAACATGATGCAATTCTTAACATCCTCAGCCGGAAATGGTTGGAGAGTCCTTGGAGCATCGGTTTCTTCAAAATCCATTGATTTGGAAGAGGTACTGCCCGGTGCACCAACGATTCTAGTCCTGGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTGGAGAGATCCTGCTCACAACTTGTTAGAATCCCTGGGAATATTCCAGCTAATGTAACAATTGATAAGGAAAACGACATTGAAACAACAGATGGGTGCTCGGTGGATGAGTTTCGATCCTTTATGGCTGTTGAGAGCTTAAACGTCAGCGTTGCGGCAGGTGTGTTTGTTCATCATTTAATTGGAAACAGTTACAAAAATGAATCATTAGAAACCTAAACCTTCATTACAGGTTAGATCAATTCATTTCTACATGATTGTGTGCAACATTACATGTCATGTTCGTCCCTTGTTTATCATCTATAAAAGGAAACAGAGTTGTCAAAACCATGTGAATTGGCTTTGCCAAAGTTTTCAAATGAAGCTACAATCAAGACATGGCCCATAAGACAACTCATCTCTTCCTGTATCTCCAACTATTTGCCTTTTTCTAGGCTTATTGTTCTTATTACCCAATCACTTGAAACCATTTTAGTTCTACGAGTCTGTTTTTCTTTTCAAATTTCTTCACTGAGAGGGAAATTTTGTTTTTTTTTGTTTTTCCAAGGAAAAAAACAAGGCTGTTCTTCGTCTTCTGGTATGCTAAGTTGTAATTTGTAATAATTGATAACCGG

mRNA sequence

CGTATCACCATCCTTCTCCATTTCTCAGTCTCTTGCCTCAACCATATCTAGCCACCGTTGAATTTCATCTCGAAACTGTGCGGCGGCGGACATTCGGCGATCAATTAACCGGAGATAAGAGACGCGCGTTTCTTCACACCCATGAGAGTTTATTCGGGACTAGATGCTCCAAATCATTTACTTCCTCAAATCTCCAAATTTTACATACAGGGCCATCTCCAAAACCCCCAATAAACCCTAGTTTGCTCACTACATTTTCCTCATCTAGGTCATTTTCAAGCTTTCACATTAATCTCTCTATGGCTAACCTTGTGAAGCTTTCAATTCGTGGTCGCCATTGTTTTCCAGAAATTAGGGATTTAGTAAGCCCCCAATTGAACTCGCTGCCTTTGAGCGTTGGGAGTTCTTTGAAATCTTTGTCCTTGAATAAATCTCGATGTTTTTCGAGTTCCTATCGTCTTAAGGACGAAGGAGTGTTATCTGGGAATAAGCTTCCTTGGTTAGCTGCAGCTAACAGTGTTGAGAAGGCAAAATGGTTGGATAAATCTACTACAAAGAAGAGAGGGGACCGATCGTCTTGGAAGGAATCGGATTTTAAGTCAAAGAGATTTGGAAAAAACGGCAATAGGTCGTCTCGGGATGAACCGGAAAAGAGATATGGCAAAGAACCAATTGGTAGTGCTAATCGTTCGACTTGGGAGGTCTCTGCTGAGAAATTTGCTAGACGAGGTATTGAGTCAGCAGATGAAAGAAGAGAAAGGAGGACAAGAATGGACAATGAAGAGCAGGAAAACGATGATATCGAAAGGAGGGGAAATTATGAAGAAGGGGAAGATGAAGAAATAGAAGCCATTGCAGACCCTAGATGGGATAAGATTAAAAGTAGATTTAGGGGAATGGTGGATGAAAGAGGTGCAACTGAGCGGCCTCAGTTTCAGAGATGGAATAAACAAGAAAGTTGGGGCAGAAAAACATGGAAGGAAGCTTCTGAATCCACTTTACCTAAAATGATTGGTGAAGGGGTTTATGGCGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGGATTGACTTGAACAATAACAATAAGAAGAAGAAGGACAAGAAAGGGTTTGAGAAAGTTCTCAAGTTGGCTGAAAAGAATGCATTAACTATCAAGGAAATATCGAAGCACGATCTCAATATGATTTCTGATAACCGACCTCATCAAGGTCTTGTGTTAGATACTTCTCCCTTGGAGATGGTTAAAATAAAAGAATTGGATCCTGTCTCACTCGAAGATGACAAAGGTTCACTTTGGTTGGCTTTGGATGAAGTTACTGACCCACAGAACTTGGGGGCAATAATAAGATCAGCTTATTTCTTTGGCGCATCGGGAGTTGTTTTATGTGCAAAAAATTCAGCTCCATTAAGTGGTGTGGTTAGTAAAGCAAGTGCTGGTTCACTTGAGTTAATGGAGCTTAGATACTGTAAGAACATGATGCAATTCTTAACATCCTCAGCCGGAAATGGTTGGAGAGTCCTTGGAGCATCGGTTTCTTCAAAATCCATTGATTTGGAAGAGGTACTGCCCGGTGCACCAACGATTCTAGTCCTGGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTGGAGAGATCCTGCTCACAACTTGTTAGAATCCCTGGGAATATTCCAGCTAATGTAACAATTGATAAGGAAAACGACATTGAAACAACAGATGGGTGCTCGGTGGATGAGTTTCGATCCTTTATGGCTGTTGAGAGCTTAAACGTCAGCGTTGCGGCAGGTGTGTTTGTTCATCATTTAATTGGAAACAGTTACAAAAATGAATCATTAGAAACCTAAACCTTCATTACAGGTTAGATCAATTCATTTCTACATGATTGTGTGCAACATTACATGTCATGTTCGTCCCTTGTTTATCATCTATAAAAGGAAACAGAGTTGTCAAAACCATGTGAATTGGCTTTGCCAAAGTTTTCAAATGAAGCTACAATCAAGACATGGCCCATAAGACAACTCATCTCTTCCTGTATCTCCAACTATTTGCCTTTTTCTAGGCTTATTGTTCTTATTACCCAATCACTTGAAACCATTTTAGTTCTACGAGTCTGTTTTTCTTTTCAAATTTCTTCACTGAGAGGGAAATTTTGTTTTTTTTTGTTTTTCCAAGGAAAAAAACAAGGCTGTTCTTCGTCTTCTGGTATGCTAAGTTGTAATTTGTAATAATTGATAACCGG

Coding sequence (CDS)

ATGGCTAACCTTGTGAAGCTTTCAATTCGTGGTCGCCATTGTTTTCCAGAAATTAGGGATTTAGTAAGCCCCCAATTGAACTCGCTGCCTTTGAGCGTTGGGAGTTCTTTGAAATCTTTGTCCTTGAATAAATCTCGATGTTTTTCGAGTTCCTATCGTCTTAAGGACGAAGGAGTGTTATCTGGGAATAAGCTTCCTTGGTTAGCTGCAGCTAACAGTGTTGAGAAGGCAAAATGGTTGGATAAATCTACTACAAAGAAGAGAGGGGACCGATCGTCTTGGAAGGAATCGGATTTTAAGTCAAAGAGATTTGGAAAAAACGGCAATAGGTCGTCTCGGGATGAACCGGAAAAGAGATATGGCAAAGAACCAATTGGTAGTGCTAATCGTTCGACTTGGGAGGTCTCTGCTGAGAAATTTGCTAGACGAGGTATTGAGTCAGCAGATGAAAGAAGAGAAAGGAGGACAAGAATGGACAATGAAGAGCAGGAAAACGATGATATCGAAAGGAGGGGAAATTATGAAGAAGGGGAAGATGAAGAAATAGAAGCCATTGCAGACCCTAGATGGGATAAGATTAAAAGTAGATTTAGGGGAATGGTGGATGAAAGAGGTGCAACTGAGCGGCCTCAGTTTCAGAGATGGAATAAACAAGAAAGTTGGGGCAGAAAAACATGGAAGGAAGCTTCTGAATCCACTTTACCTAAAATGATTGGTGAAGGGGTTTATGGCGTTGGACCAGTTTTGGCTGCTTTGTCAGCTGGAAGAAGAGAGTTCTATGCATTGTATATGCAAGAAGGGATTGACTTGAACAATAACAATAAGAAGAAGAAGGACAAGAAAGGGTTTGAGAAAGTTCTCAAGTTGGCTGAAAAGAATGCATTAACTATCAAGGAAATATCGAAGCACGATCTCAATATGATTTCTGATAACCGACCTCATCAAGGTCTTGTGTTAGATACTTCTCCCTTGGAGATGGTTAAAATAAAAGAATTGGATCCTGTCTCACTCGAAGATGACAAAGGTTCACTTTGGTTGGCTTTGGATGAAGTTACTGACCCACAGAACTTGGGGGCAATAATAAGATCAGCTTATTTCTTTGGCGCATCGGGAGTTGTTTTATGTGCAAAAAATTCAGCTCCATTAAGTGGTGTGGTTAGTAAAGCAAGTGCTGGTTCACTTGAGTTAATGGAGCTTAGATACTGTAAGAACATGATGCAATTCTTAACATCCTCAGCCGGAAATGGTTGGAGAGTCCTTGGAGCATCGGTTTCTTCAAAATCCATTGATTTGGAAGAGGTACTGCCCGGTGCACCAACGATTCTAGTCCTGGGCAGTGAAGGCACTGGTTTGAGGCCCTTGGTGGAGAGATCCTGCTCACAACTTGTTAGAATCCCTGGGAATATTCCAGCTAATGTAACAATTGATAAGGAAAACGACATTGAAACAACAGATGGGTGCTCGGTGGATGAGTTTCGATCCTTTATGGCTGTTGAGAGCTTAAACGTCAGCGTTGCGGCAGGTGTGTTTGTTCATCATTTAATTGGAAACAGTTACAAAAATGAATCATTAGAAACCTAA

Protein sequence

MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVLSGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKENDIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET
Homology
BLAST of IVF0013022 vs. ExPASy Swiss-Prot
Match: Q76NT0 (rRNA methyltransferase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=mrm1 PE=3 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 7.2e-34
Identity = 96/291 (32.99%), Postives = 149/291 (51.20%), Query Frame = 0

Query: 265 QEGIDLNNNNKKKK-------DKK---GFEKVLKLAEKNALTIKEISKHDLNMISDNRPH 324
           +E  D NNNN  KK       D+K     ++++K + K  + ++ ++K  L+  S  RPH
Sbjct: 262 EEEEDNNNNNNIKKTTFNRYNDRKNLVALKEIVKNSFKLKIPVRSVNKGTLDSFSKGRPH 321

Query: 325 QGLVLDTSPLEMVKIKELDPVSLED-----------------------DKGSLWLALDEV 384
           QG++LD SPL ++ I  L+   + +                       DK  LWL LDE+
Sbjct: 322 QGIILDASPLTLLNIDFLEKFDINERVNKRMNGGGGGGGGGGGNKINNDKYPLWLVLDEL 381

Query: 385 TDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTS 444
            DPQN+GAIIR+  FF   GVV+  KNS+P++   SK+S+G+ E   +   +++  FL S
Sbjct: 382 WDPQNVGAIIRNCSFFNIDGVVISNKNSSPITPAASKSSSGACESFVINRTESIEGFLKS 441

Query: 445 SAGNGWRVLGASV-----SSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIP 504
           S  NGW V+G S+     +   +D+ ++    PTIL+LG+EG GL+P V   C++ ++I 
Sbjct: 442 SQRNGWNVVGTSLDDLNENQPCLDINQIKLDQPTILILGNEGFGLKPSVLEICNKTIKIV 501

Query: 505 GNIPANVTIDKENDIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGN 518
           G    N  ID                       SLNVSV +G+ +H L+ +
Sbjct: 502 G---GNYKID-----------------------SLNVSVTSGILIHTLLSS 526

BLAST of IVF0013022 vs. ExPASy Swiss-Prot
Match: O94631 (rRNA methyltransferase 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mrm1 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 1.1e-31
Identity = 111/319 (34.80%), Postives = 157/319 (49.22%), Query Frame = 0

Query: 229 ASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVL- 288
           + E  L K   E V+G   V  AL+  +RE  ALY+Q   D+++          FE+ L 
Sbjct: 21  SKERRLIKEDSEFVFGTHSVKNALATRKRECRALYVQNA-DIHSE---------FEEFLN 80

Query: 289 KLAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKEL--------------- 348
           KL  K  + IK ++K  LN I+  RPH G+VL+ S L +  I +L               
Sbjct: 81  KLQYK--IPIKSVNKEHLNQITACRPHNGVVLEASSLNVPTISDLLFPAGEEYNNKNGQD 140

Query: 349 --------DPVSLEDDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSG 408
                      S  D+   L++ +D +TDPQN+GA+IRSAY  GA G++L  K++  LS 
Sbjct: 141 SPHNDLNEGKSSSSDNYPPLYVYVDGITDPQNMGAVIRSAYILGAKGILLSKKHNTFLSP 200

Query: 409 VVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVLGASVSSKS--------IDLEEVLP 468
           VVSKASAG+LE+  + + KN M FL +S   GW+V+G   +             ++  L 
Sbjct: 201 VVSKASAGALEVFNISHVKNPMVFLRNSVLKGWKVIGTKPALPDNKDQIYTPHKIKTELM 260

Query: 469 GAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKENDIETTDGCSVDEFRSFM 516
             P ILVLGSE  GLR  +   CS  V IPG                      D++    
Sbjct: 261 NEPKILVLGSE-KGLRTNILTQCSHYVSIPGG---------------------DKY---- 300

BLAST of IVF0013022 vs. ExPASy Swiss-Prot
Match: Q6IN84 (rRNA methyltransferase 1, mitochondrial OS=Homo sapiens OX=9606 GN=MRM1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 3.7e-30
Identity = 95/289 (32.87%), Postives = 144/289 (49.83%), Query Frame = 0

Query: 240 EGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFE----KVLKLAEKNAL 299
           E ++G+ P L AL A RR    L +Q G            K G +    ++L++AE   +
Sbjct: 50  ELLFGMTPCLLALQAARRSVARLLLQAG------------KAGLQGKRAELLRMAEARDI 109

Query: 300 TIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQ 359
            +    +  L+ +   + HQG+ ++ SPL     +E    S  DD   LWL LD + DP+
Sbjct: 110 PVLRPRRQKLDTMCRYQVHQGVCMEVSPLRPRPWREAGEASPGDDPQQLWLVLDGIQDPR 169

Query: 360 NLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGN 419
           N GA++RSA+F G   V+   +NS PL+ VVSK+SAG++E+M++    ++  FL + A  
Sbjct: 170 NFGAVLRSAHFLGVDKVITSRRNSCPLTPVVSKSSAGAMEVMDVFSTDDLTGFLQTKAQQ 229

Query: 420 GWRVLGASVSSKSIDLE----------EVLPGAPTILVLGSEGTGLRPLVERSCSQLVRI 479
           GW V G      + D +          E L   PT+LVLG+EG+GL   V+ SC  L+ I
Sbjct: 230 GWLVAGTVGCPSTEDPQSSEIPIMSCLEFLWERPTLLVLGNEGSGLSQEVQASCQLLLTI 289

Query: 480 PGNIPANVTIDKENDIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHL 515
              +P           +   G           +ESLNVSVAAG+ +H +
Sbjct: 290 ---LPRR---------QLPPG-----------LESLNVSVAAGILLHSI 303

BLAST of IVF0013022 vs. ExPASy Swiss-Prot
Match: Q99J25 (rRNA methyltransferase 1, mitochondrial OS=Mus musculus OX=10090 GN=Mrm1 PE=2 SV=2)

HSP 1 Score: 133.7 bits (335), Expect = 6.3e-30
Identity = 97/291 (33.33%), Postives = 143/291 (49.14%), Query Frame = 0

Query: 240 EGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFE----KVLKLAEKNAL 299
           E ++G+ P L AL A RR    L +Q G            K G +    ++L++AE   +
Sbjct: 48  ERLFGLSPCLLALRAARRRVARLLLQAG------------KAGLQGERAELLRVAEARGI 107

Query: 300 TIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQ 359
            +    +  L+ +   + HQG+ ++ SPL      E    S  DD   LWL L+ + DP+
Sbjct: 108 PVLRPRRQKLDALCGYQVHQGVCMEVSPLRPRPCDEAADTSSGDDPQQLWLVLEGLQDPR 167

Query: 360 NLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGN 419
           NLGA++RSA+F G   V+   +NS PL+ VVSKASAG++E+M++    ++  FL + A  
Sbjct: 168 NLGAVMRSAHFLGVDRVITSQRNSCPLTPVVSKASAGAMEVMDVFATPDLPGFLQAKAQQ 227

Query: 420 GWRVLGA--------SVSSK--SIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRI 479
           GW V+G         S SSK       E +   PT+LVLGSEG+GL   V  SC  L+ I
Sbjct: 228 GWLVVGTVGCPGPEISQSSKVPITSCLEFVWDRPTLLVLGSEGSGLSQEVFASCQLLLTI 287

Query: 480 --PGNIPANVTIDKENDIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHL 515
               ++P                           +ESLNVSVA G+ +H +
Sbjct: 288 LPRRHLPP-------------------------GLESLNVSVATGILLHSI 301

BLAST of IVF0013022 vs. ExPASy Swiss-Prot
Match: A4T5L0 (Uncharacterized tRNA/rRNA methyltransferase Mflv_1447 OS=Mycolicibacterium gilvum (strain PYR-GCK) OX=350054 GN=Mflv_1447 PE=3 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 6.7e-24
Identity = 89/270 (32.96%), Postives = 130/270 (48.15%), Query Frame = 0

Query: 240 EGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE 299
           E V G  PV+  L AG     ALY+  G D         D++  E V ++A    ++I E
Sbjct: 71  EIVLGRNPVVECLRAG-VPATALYVALGTD--------ADERLTESV-QMAADRGISILE 130

Query: 300 ISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA 359
           + +HDL+ ++ N  HQG+ L   P       +L   +  D   +L +ALD ++DP+NLGA
Sbjct: 131 VQRHDLDRMAANGLHQGIALQVPPYAYAHPDDLLRDAKSDAAPALLVALDNISDPRNLGA 190

Query: 360 IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRV 419
           I+RS   FG  GVVL  + SA ++ V  + SAG+     +    N+ + L   A  G +V
Sbjct: 191 IVRSVAAFGGHGVVLPQRRSASVTAVAWRTSAGAAARTPVARATNLNRTLKQYADAGLQV 250

Query: 420 LGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEN 479
           +G      +  ++E+    PTI+V+GSEG GL  LV  +C ++V IP   P         
Sbjct: 251 VGLDAGGDT-TIDEIDGLTPTIVVVGSEGKGLSRLVRENCDRIVSIPMAGP--------- 302

Query: 480 DIETTDGCSVDEFRSFMAVESLNVSVAAGV 510
                              ESLN SVAAGV
Sbjct: 311 ------------------TESLNASVAAGV 302

BLAST of IVF0013022 vs. ExPASy TrEMBL
Match: A0A5D3CIS9 (Putative tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002930 PE=4 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.8e-293
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL
Sbjct: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120
           SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY
Sbjct: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120

Query: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180
           GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE
Sbjct: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180

Query: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240
           EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE
Sbjct: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240

Query: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300
           GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI
Sbjct: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300

Query: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360
           SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI
Sbjct: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360

Query: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420
           IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL
Sbjct: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420

Query: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480
           GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND
Sbjct: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480

Query: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 527
           IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET
Sbjct: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

BLAST of IVF0013022 vs. ExPASy TrEMBL
Match: A0A1S3BQ13 (uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 OS=Cucumis melo OX=3656 GN=LOC103492262 PE=4 SV=1)

HSP 1 Score: 1017.7 bits (2630), Expect = 1.8e-293
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL
Sbjct: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120
           SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY
Sbjct: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120

Query: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180
           GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE
Sbjct: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180

Query: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240
           EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE
Sbjct: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240

Query: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300
           GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI
Sbjct: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300

Query: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360
           SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI
Sbjct: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360

Query: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420
           IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL
Sbjct: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420

Query: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480
           GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND
Sbjct: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480

Query: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 527
           IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET
Sbjct: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

BLAST of IVF0013022 vs. ExPASy TrEMBL
Match: A0A0A0LW97 (SpoU_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G574920 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 9.4e-279
Identity = 503/527 (95.45%), Postives = 515/527 (97.72%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           MAN VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSL+S+SLNKSRCFSSSYRLKDEGVL
Sbjct: 1   MANFVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLRSVSLNKSRCFSSSYRLKDEGVL 60

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120
           SGNKLPWLAAA+SVEKAKWLDKSTTKKRGDRS WKESDFKSKRFGKNGNRSSRDEPEKRY
Sbjct: 61  SGNKLPWLAAASSVEKAKWLDKSTTKKRGDRSPWKESDFKSKRFGKNGNRSSRDEPEKRY 120

Query: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDIERRGNYEEGED 180
           GKEPIGSANRSTWEVSAEKFARR IE ADE+RE RRTRMDNEEQ++DDIERRG+YEE ED
Sbjct: 121 GKEPIGSANRSTWEVSAEKFARRSIELADEKRESRRTRMDNEEQKDDDIERRGSYEEVED 180

Query: 181 EEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIG 240
           EE EAIADPRWDKIKSRFRGMVDERGATERP+FQRWNKQESWGRKTWKEASESTLPKMIG
Sbjct: 181 EETEAIADPRWDKIKSRFRGMVDERGATERPEFQRWNKQESWGRKTWKEASESTLPKMIG 240

Query: 241 EGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE 300
           EGVYGVGPVLAALSAGRREFYALY+QEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE
Sbjct: 241 EGVYGVGPVLAALSAGRREFYALYVQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE 300

Query: 301 ISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA 360
           ISKHDLNMISDNRPHQGLVLD SPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA
Sbjct: 301 ISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA 360

Query: 361 IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRV 420
           IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSA NGWRV
Sbjct: 361 IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGWRV 420

Query: 421 LGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEN 480
           LGASVSSKSIDL+EVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIP +VTID EN
Sbjct: 421 LGASVSSKSIDLKEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTIDTEN 480

Query: 481 DIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 527
           DIETTDG SVDEFRSFMAVESLNVSVAAGVFVHHL+GNSYKNESLET
Sbjct: 481 DIETTDGRSVDEFRSFMAVESLNVSVAAGVFVHHLVGNSYKNESLET 527

BLAST of IVF0013022 vs. ExPASy TrEMBL
Match: A0A6J1HPM2 (uncharacterized protein LOC111465489 OS=Cucurbita maxima OX=3661 GN=LOC111465489 PE=4 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 5.0e-224
Identity = 426/541 (78.74%), Postives = 456/541 (84.29%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           +ANL+ LS+R  HC PEIR  VSP+L+S  L+  SSL      KSR +SSS+ +KDEGV 
Sbjct: 51  LANLMNLSMRSHHCSPEIRKFVSPKLSSPALTAESSL------KSRTYSSSHSIKDEGVK 110

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKES-----------DFKSKRFGKNGN 120
           S NKLPWLAA NS EKAKWLDKSTTKKRGDRSSWKES           D+  K FGK GN
Sbjct: 111 SRNKLPWLAATNSFEKAKWLDKSTTKKRGDRSSWKESNSGSEKMRSSWDYSKKTFGKRGN 170

Query: 121 RSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDI 180
           R S DEPEKRYGK P+GSA RS+WEVSAEKF  +G+E ADERRE RR R+DNEEQ N D+
Sbjct: 171 RPSGDEPEKRYGKAPVGSAYRSSWEVSAEKFGTQGMEYADERRESRRGRVDNEEQRNRDV 230

Query: 181 ERRGNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKE 240
                  E EDEE EAIADPRWDKIKS+F          ER +FQRWNKQESW RKTWKE
Sbjct: 231 -------EVEDEETEAIADPRWDKIKSKF----------ERHEFQRWNKQESWSRKTWKE 290

Query: 241 ASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLK 300
           ASESTLPKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+
Sbjct: 291 ASESTLPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLR 350

Query: 301 LAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLAL 360
           LAE+NALT+KEISKHDLNM+SDNRPHQGLVLD SPLEMVKI ELDPVSLE+DKGSLWLAL
Sbjct: 351 LAERNALTVKEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGSLWLAL 410

Query: 361 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 420
           DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF
Sbjct: 411 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 470

Query: 421 LTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN 480
           LTSSA NGWRVLGASVSSKSIDLEEV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGN
Sbjct: 471 LTSSAENGWRVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGN 530

Query: 481 IPANVTIDKENDIETTD---GCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLE 527
           IP +V ID++NDIETT      SVDEFRSFMAVESLNV VAAGV VHHLIG+SYK ES E
Sbjct: 531 IPVDVAIDRDNDIETTQPTKESSVDEFRSFMAVESLNVGVAAGVLVHHLIGSSYKKESYE 568

BLAST of IVF0013022 vs. ExPASy TrEMBL
Match: A0A6J1FCE1 (rRNA methyltransferase 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111444126 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.8e-221
Identity = 420/532 (78.95%), Postives = 456/532 (85.71%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           +ANL+ LSIR +HC PEIR LVS +L+   L++ SSL      KSR +SSS+ +KDEGV 
Sbjct: 51  LANLMSLSIRSQHCSPEIRKLVSRKLSLPALTIESSL------KSRTYSSSHSIKDEGVK 110

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKS--KRFGKNGNRSSRDEPEK 120
           S +KLPWLAA NS EKA+W DKSTTKKRGDRSSWKES+  S  K FGK GNR+S DEPEK
Sbjct: 111 SRDKLPWLAATNSFEKARWSDKSTTKKRGDRSSWKESNSGSEKKTFGKRGNRASGDEPEK 170

Query: 121 RYGKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDIERRGNYEEG 180
           RYGK P+GS  RS+WEVSAEKFA + +E A+ERRE RR R+DNEEQ N D+       E 
Sbjct: 171 RYGKAPVGSTYRSSWEVSAEKFATQRMEYAEERRESRRGRVDNEEQRNRDV-------EV 230

Query: 181 EDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKM 240
           EDEE EAIADPRWDKIK++F          ERP+FQRWNKQESW RKTWKEASESTLPKM
Sbjct: 231 EDEETEAIADPRWDKIKTKF----------ERPEFQRWNKQESWSRKTWKEASESTLPKM 290

Query: 241 IGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTI 300
           +GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+LAE+NALT+
Sbjct: 291 VGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLRLAERNALTV 350

Query: 301 KEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNL 360
           KEISKHDLNM+SDNRPHQGLVLD SPLEMVKI ELDPVSLE+DKG LWLALDEVTDPQNL
Sbjct: 351 KEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGLLWLALDEVTDPQNL 410

Query: 361 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGW 420
           GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSA NGW
Sbjct: 411 GAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGW 470

Query: 421 RVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDK 480
           RVLGASVSSKSIDLEEV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGNIP ++  DK
Sbjct: 471 RVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGNIPVDIATDK 530

Query: 481 ENDIETTD---GCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 527
           END ETT      SVDEFRSFMAVESLNVSVAAGV VHHLIG++YKNES ET
Sbjct: 531 ENDNETTQPTKESSVDEFRSFMAVESLNVSVAAGVLVHHLIGSNYKNESYET 559

BLAST of IVF0013022 vs. NCBI nr
Match: XP_008450781.1 (PREDICTED: uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 [Cucumis melo] >TYK10149.1 putative tRNA/rRNA methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 1014 bits (2622), Expect = 0.0
Identity = 526/526 (100.00%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL
Sbjct: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120
           SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY
Sbjct: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120

Query: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180
           GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE
Sbjct: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRERRTRMDNEEQENDDIERRGNYEEGEDE 180

Query: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240
           EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE
Sbjct: 181 EIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGE 240

Query: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300
           GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI
Sbjct: 241 GVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEI 300

Query: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360
           SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI
Sbjct: 301 SKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAI 360

Query: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420
           IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL
Sbjct: 361 IRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVL 420

Query: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480
           GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND
Sbjct: 421 GASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEND 480

Query: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526
           IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET
Sbjct: 481 IETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526

BLAST of IVF0013022 vs. NCBI nr
Match: XP_004135672.1 (uncharacterized protein LOC101220884 [Cucumis sativus] >XP_031739747.1 uncharacterized protein LOC101220884 [Cucumis sativus] >KGN66175.1 hypothetical protein Csa_007609 [Cucumis sativus])

HSP 1 Score: 965 bits (2495), Expect = 0.0
Identity = 503/527 (95.45%), Postives = 515/527 (97.72%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           MAN VKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSL+S+SLNKSRCFSSSYRLKDEGVL
Sbjct: 1   MANFVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLRSVSLNKSRCFSSSYRLKDEGVL 60

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKESDFKSKRFGKNGNRSSRDEPEKRY 120
           SGNKLPWLAAA+SVEKAKWLDKSTTKKRGDRS WKESDFKSKRFGKNGNRSSRDEPEKRY
Sbjct: 61  SGNKLPWLAAASSVEKAKWLDKSTTKKRGDRSPWKESDFKSKRFGKNGNRSSRDEPEKRY 120

Query: 121 GKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDIERRGNYEEGED 180
           GKEPIGSANRSTWEVSAEKFARR IE ADE+RE RRTRMDNEEQ++DDIERRG+YEE ED
Sbjct: 121 GKEPIGSANRSTWEVSAEKFARRSIELADEKRESRRTRMDNEEQKDDDIERRGSYEEVED 180

Query: 181 EEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIG 240
           EE EAIADPRWDKIKSRFRGMVDERGATERP+FQRWNKQESWGRKTWKEASESTLPKMIG
Sbjct: 181 EETEAIADPRWDKIKSRFRGMVDERGATERPEFQRWNKQESWGRKTWKEASESTLPKMIG 240

Query: 241 EGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE 300
           EGVYGVGPVLAALSAGRREFYALY+QEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE
Sbjct: 241 EGVYGVGPVLAALSAGRREFYALYVQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKE 300

Query: 301 ISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA 360
           ISKHDLNMISDNRPHQGLVLD SPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA
Sbjct: 301 ISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGA 360

Query: 361 IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRV 420
           IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSA NGWRV
Sbjct: 361 IIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAENGWRV 420

Query: 421 LGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKEN 480
           LGASVSSKSIDL+EVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIP +VTID EN
Sbjct: 421 LGASVSSKSIDLKEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPVDVTIDTEN 480

Query: 481 DIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLET 526
           DIETTDG SVDEFRSFMAVESLNVSVAAGVFVHHL+GNSYKNESLET
Sbjct: 481 DIETTDGRSVDEFRSFMAVESLNVSVAAGVFVHHLVGNSYKNESLET 527

BLAST of IVF0013022 vs. NCBI nr
Match: XP_038880007.1 (uncharacterized protein LOC120071698 [Benincasa hispida] >XP_038880008.1 uncharacterized protein LOC120071698 [Benincasa hispida])

HSP 1 Score: 864 bits (2233), Expect = 3.55e-312
Identity = 462/540 (85.56%), Postives = 485/540 (89.81%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           + NL+KLSIRGRHCFPEIRDLVSPQLNS  LS+GSSL+S S  KSR F SSY LKDEGVL
Sbjct: 57  LVNLMKLSIRGRHCFPEIRDLVSPQLNSSALSIGSSLRSFSSKKSRRFLSSYPLKDEGVL 116

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSS---WKESDFKSKRF-----------GK 120
           SGNKLPWLAAANS+EKAKW DKST +K GDRSS   WKESD +S R            G+
Sbjct: 117 SGNKLPWLAAANSLEKAKWSDKSTKRKGGDRSSRSSWKESDSRSDRMRSSWDYSTKTSGE 176

Query: 121 NGNRSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQEN 180
            GNRSSRDEPEKR GK PIGSANRSTWEVSAEKFAR+GI+SADERRE RRT +DNEEQ+N
Sbjct: 177 KGNRSSRDEPEKRNGKAPIGSANRSTWEVSAEKFARQGIKSADERRESRRTIVDNEEQQN 236

Query: 181 DDIERRGNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKT 240
           DDIER G+YEE EDEE EAIADPRWDKIKSRF+GMVDER A ERP+FQRWNKQESWGRKT
Sbjct: 237 DDIERGGSYEEVEDEETEAIADPRWDKIKSRFKGMVDERAAPERPEFQRWNKQESWGRKT 296

Query: 241 WKEASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEK 300
           W+EASESTLPKMIGEGVYG+GPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEK
Sbjct: 297 WREASESTLPKMIGEGVYGIGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEK 356

Query: 301 VLKLAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLW 360
           VL+LAEKNALTIKEISKHDLNMISDNRPHQGLVLD SPLEMVKIKELDPV LE+DK SLW
Sbjct: 357 VLRLAEKNALTIKEISKHDLNMISDNRPHQGLVLDASPLEMVKIKELDPVLLEEDKDSLW 416

Query: 361 LALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNM 420
           LALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNM
Sbjct: 417 LALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNM 476

Query: 421 MQFLTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRI 480
           MQFLTSSA NGWRVLGASVSSKSI L E+ PGAPTILVLGSEGTGLRPLVERSCSQLVRI
Sbjct: 477 MQFLTSSAENGWRVLGASVSSKSIALNEIAPGAPTILVLGSEGTGLRPLVERSCSQLVRI 536

Query: 481 PGNIPANVTIDKENDIETTDG---CSVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNE 522
           PGNIP  VTIDKENDIETT     C VDEF+SFMAVESLNVSVAAGV +HHLIGN+YKNE
Sbjct: 537 PGNIPVEVTIDKENDIETTQPSNVCLVDEFQSFMAVESLNVSVAAGVLIHHLIGNNYKNE 596

BLAST of IVF0013022 vs. NCBI nr
Match: KAG7021321.1 (rRNA methyltransferase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 787 bits (2032), Expect = 7.49e-282
Identity = 425/541 (78.56%), Postives = 462/541 (85.40%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           +ANL+ LSIR  HC PEIR LVSP+L+S  L++ SSLKS      R +SSS+ +KDEGV 
Sbjct: 60  LANLMNLSIRSHHCSPEIRKLVSPKLSSPALTIESSLKS------RTYSSSHSIKDEGVK 119

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKES-----------DFKSKRFGKNGN 120
           S +KLPWLAA NS EKA+WL KSTTKKRGDRSSWKES           D+  K FGK GN
Sbjct: 120 SRDKLPWLAATNSFEKARWLGKSTTKKRGDRSSWKESNSGSEKMRSSWDYSKKTFGKRGN 179

Query: 121 RSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDI 180
           R+S DEPEKRYGK P+GS +RS+WEVSAEKFA + +E A+ERRE RR R+DNEEQ+N D+
Sbjct: 180 RASGDEPEKRYGKAPVGSTDRSSWEVSAEKFATQRMEYAEERRESRRGRVDNEEQQNRDV 239

Query: 181 ERRGNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKE 240
           E        EDEE EAIADPRWDKIKS+F          ERP+FQRWNKQESW RKTWKE
Sbjct: 240 E-------VEDEETEAIADPRWDKIKSKF----------ERPEFQRWNKQESWSRKTWKE 299

Query: 241 ASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLK 300
           ASESTLPKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+
Sbjct: 300 ASESTLPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLR 359

Query: 301 LAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLAL 360
           LAE+NALT+KEISKHDLNM+SDNRPHQGLVLD SPLEMVKI ELDPVSLE+DKGSLWLAL
Sbjct: 360 LAERNALTVKEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGSLWLAL 419

Query: 361 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 420
           DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF
Sbjct: 420 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 479

Query: 421 LTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN 480
           LTSSA NGWRVLGASVSSKSIDLEEV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGN
Sbjct: 480 LTSSAENGWRVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGN 539

Query: 481 IPANVTIDKENDIETTDGC---SVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLE 526
           IP ++ +DKENDIETT      SVDEFRSFMAVESLNVSVAAGV VHHLIG++YKNES E
Sbjct: 540 IPVDIAMDKENDIETTQPTKESSVDEFRSFMAVESLNVSVAAGVLVHHLIGSNYKNESYE 577

BLAST of IVF0013022 vs. NCBI nr
Match: XP_022965658.1 (uncharacterized protein LOC111465489 [Cucurbita maxima])

HSP 1 Score: 784 bits (2024), Expect = 8.88e-281
Identity = 426/541 (78.74%), Postives = 456/541 (84.29%), Query Frame = 0

Query: 1   MANLVKLSIRGRHCFPEIRDLVSPQLNSLPLSVGSSLKSLSLNKSRCFSSSYRLKDEGVL 60
           +ANL+ LS+R  HC PEIR  VSP+L+S  L+  SSLKS      R +SSS+ +KDEGV 
Sbjct: 51  LANLMNLSMRSHHCSPEIRKFVSPKLSSPALTAESSLKS------RTYSSSHSIKDEGVK 110

Query: 61  SGNKLPWLAAANSVEKAKWLDKSTTKKRGDRSSWKES-----------DFKSKRFGKNGN 120
           S NKLPWLAA NS EKAKWLDKSTTKKRGDRSSWKES           D+  K FGK GN
Sbjct: 111 SRNKLPWLAATNSFEKAKWLDKSTTKKRGDRSSWKESNSGSEKMRSSWDYSKKTFGKRGN 170

Query: 121 RSSRDEPEKRYGKEPIGSANRSTWEVSAEKFARRGIESADERRE-RRTRMDNEEQENDDI 180
           R S DEPEKRYGK P+GSA RS+WEVSAEKF  +G+E ADERRE RR R+DNEEQ N D+
Sbjct: 171 RPSGDEPEKRYGKAPVGSAYRSSWEVSAEKFGTQGMEYADERRESRRGRVDNEEQRNRDV 230

Query: 181 ERRGNYEEGEDEEIEAIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKE 240
           E        EDEE EAIADPRWDKIKS+F          ER +FQRWNKQESW RKTWKE
Sbjct: 231 E-------VEDEETEAIADPRWDKIKSKF----------ERHEFQRWNKQESWSRKTWKE 290

Query: 241 ASESTLPKMIGEGVYGVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLK 300
           ASESTLPKM+GEGVYGVGPVLAALSAGRREFYALYMQEG+DLNNNNKKKKDKKGFEKVL+
Sbjct: 291 ASESTLPKMVGEGVYGVGPVLAALSAGRREFYALYMQEGLDLNNNNKKKKDKKGFEKVLR 350

Query: 301 LAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLAL 360
           LAE+NALT+KEISKHDLNM+SDNRPHQGLVLD SPLEMVKI ELDPVSLE+DKGSLWLAL
Sbjct: 351 LAERNALTVKEISKHDLNMLSDNRPHQGLVLDASPLEMVKIMELDPVSLEEDKGSLWLAL 410

Query: 361 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 420
           DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF
Sbjct: 411 DEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQF 470

Query: 421 LTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGN 480
           LTSSA NGWRVLGASVSSKSIDLEEV PGAPTILVLGSEGTGLRPLVERSCSQLV+IPGN
Sbjct: 471 LTSSAENGWRVLGASVSSKSIDLEEVPPGAPTILVLGSEGTGLRPLVERSCSQLVKIPGN 530

Query: 481 IPANVTIDKENDIETTDGC---SVDEFRSFMAVESLNVSVAAGVFVHHLIGNSYKNESLE 526
           IP +V ID++NDIETT      SVDEFRSFMAVESLNV VAAGV VHHLIG+SYK ES E
Sbjct: 531 IPVDVAIDRDNDIETTQPTKESSVDEFRSFMAVESLNVGVAAGVLVHHLIGSSYKKESYE 568

BLAST of IVF0013022 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 468.0 bits (1203), Expect = 1.0e-131
Identity = 275/514 (53.50%), Postives = 350/514 (68.09%), Query Frame = 0

Query: 36  SLKSLSLNKSRCFSSSYRLK---DEGVLSGNKLPWLAAANSVEKAKWLDKSTTKKRGDRS 95
           S  + S  KS   S +YR K     G  SG+ + W+ +     + ++       +RGDR 
Sbjct: 90  SSSNSSRGKSGLGSKAYRDKRSGGSGRSSGDSI-WVKSDEKPVEERF-------QRGDRP 149

Query: 96  SWKESD----------FKSKRFGKNGNRSS----RDEPEKRYGKEPIGSANRSTWEVSAE 155
           SW++ D           +S+ +G+  NR S    RD+      +E       S W  + +
Sbjct: 150 SWEKRDGRNDSGSDRRSRSRGYGETRNRDSFRGRRDDRISEVEEESKKGGGNSIWVANDD 209

Query: 156 KFARRGIESADERRERRTRMDNEEQENDDIERRGN---YEEGEDEEI------------E 215
           K A+        R   R+  D+  +  +    RG+    EE E+E +            E
Sbjct: 210 KPAK----EQSPRVNNRSSWDDRTRNQNSFSARGDDRITEEAEEETMNHAPEDGDGIVEE 269

Query: 216 AIADPRWDKIKSRFRGMVDERGATERPQFQRWNKQESWGRKTWKEASESTLPKMIGEGVY 275
              + RW +IK+RF          +   ++ WN+QESWGRKTW+EA+ES++P++ GE VY
Sbjct: 270 EPDNTRWSEIKNRFNRYDVRDQGRDDAAYRNWNRQESWGRKTWQEATESSVPRLEGEVVY 329

Query: 276 GVGPVLAALSAGRREFYALYMQEGIDLNNNNKKKKDKKGFEKVLKLAEKNALTIKEISKH 335
           GV PVLAALS GRREFYALY+QEG+DL++NN+KKKDKKGFE+VLK++EK  L IKE SKH
Sbjct: 330 GVSPVLAALSVGRREFYALYVQEGLDLSSNNRKKKDKKGFERVLKISEKLGLNIKETSKH 389

Query: 336 DLNMISDNRPHQGLVLDTSPLEMVKIKELDPVSLEDDKGSLWLALDEVTDPQNLGAIIRS 395
           DLNM++DNRPHQGLVLD SPLE+VK+KELDP+S E++K SLW+ALDEVTDPQNLGAIIRS
Sbjct: 390 DLNMVADNRPHQGLVLDASPLELVKVKELDPISSEEEKYSLWVALDEVTDPQNLGAIIRS 449

Query: 396 AYFFGASGVVLCAKNSAPLSGVVSKASAGSLELMELRYCKNMMQFLTSSAGNGWRVLGAS 455
           AYFFGA+GVV+CAKNSAPLS VVSKASAGSLE+MELRYCKNMMQFL +SA NGWRV+G S
Sbjct: 450 AYFFGATGVVVCAKNSAPLSAVVSKASAGSLEVMELRYCKNMMQFLEASAENGWRVVGGS 509

Query: 456 VSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKENDIET 515
           VS K++ L EVLPG+PTILVLG+EGTGLRPLVERSC+ LVRI GN+P  V + + +D E 
Sbjct: 510 VSPKAVALNEVLPGSPTILVLGNEGTGLRPLVERSCTDLVRISGNMPNEVAVTESDDAEG 569

Query: 516 TDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIGN 518
                 + FRSF+AVESLNVSVAAG+F+HHLIGN
Sbjct: 570 ------EGFRSFLAVESLNVSVAAGLFLHHLIGN 585

BLAST of IVF0013022 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 52.0 bits (123), Expect = 1.7e-06
Identity = 64/259 (24.71%), Postives = 105/259 (40.54%), Query Frame = 0

Query: 274 NKKKKDKKGFEKV-LKLAEKNALTIKEISKHDLNMISDNRPHQGLVLDTSPLEMVKIKEL 333
           +++ K  +G E + +++   ++L +K++S      +      + + L   P     +K+ 
Sbjct: 127 HEEAKIPQGLESLSIRIVRVSSLVMKKLSG-----VQSTESVEAIALMRIPSSFTDLKDD 186

Query: 334 DPVSLEDDK----GSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSK 393
             +  + +K        L LD + DP NLG ++RSA  F   G  L      P +    +
Sbjct: 187 KDIITDCNKWFPSAHRVLVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALR 246

Query: 394 ASAGS-------------LELMELRYCKNMMQFLTSSAGNGWRVLGASVSSKSIDLEEVL 453
           AS G+             L+L+E  +    M+ L        + L   VS  S++  + L
Sbjct: 247 ASRGASFQLPIVSGNWNHLKLLENEF---QMKLLAGHPATTTQKL-KPVSKLSVEFAQSL 306

Query: 454 PGAPTILVLGSEGTGLRPLVERSCSQLVRIPGNIPANVTIDKENDIETTDGCSVDEFRSF 513
              P  L+LGSEG GL     + C  LV IP                        +F   
Sbjct: 307 AEKPLCLILGSEGNGLSEQARKVC-VLVSIP---------------------MAGDF--- 348

Query: 514 MAVESLNVSVAAGVFVHHL 515
              ESLNVSVA G+F++ L
Sbjct: 367 ---ESLNVSVAGGIFLYML 348

BLAST of IVF0013022 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 47.8 bits (112), Expect = 3.2e-05
Identity = 38/181 (20.99%), Postives = 77/181 (42.54%), Query Frame = 0

Query: 337 LEDDKGSLWLALDEVTDPQNLGAIIRSAYFFGASGVVLCAKNSAPLSGVVSKASAGSLEL 396
           +E+   S+ L ++ ++D  N+ A  RSA   G   V + + +S+         S G+ + 
Sbjct: 148 VENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKW 207

Query: 397 MELRYCKNMMQFLTSSAGNGWRVLGASVSSKSIDLEEVLPGAPTILVLGSEGTGLRPLVE 456
           +++ +     +        G+R+    +   ++ + ++    PT +V+G+EG G    + 
Sbjct: 208 LDIEFWDTPKECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRG----IS 267

Query: 457 RSCSQLVRIPGNIPANVTIDKENDIETTDGCSVDEFRSFMAVESLNVSVAAGVFVHHLIG 516
               +L  +  +IP N  +D                       S NVSVAAG+ +HH + 
Sbjct: 268 DEALELSDLRCSIPMNGMVD-----------------------SFNVSVAAGILMHHAVS 301

Query: 517 N 518
           +
Sbjct: 328 D 301

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q76NT07.2e-3432.99rRNA methyltransferase 1, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=... [more]
O946311.1e-3134.80rRNA methyltransferase 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972... [more]
Q6IN843.7e-3032.87rRNA methyltransferase 1, mitochondrial OS=Homo sapiens OX=9606 GN=MRM1 PE=1 SV=... [more]
Q99J256.3e-3033.33rRNA methyltransferase 1, mitochondrial OS=Mus musculus OX=10090 GN=Mrm1 PE=2 SV... [more]
A4T5L06.7e-2432.96Uncharacterized tRNA/rRNA methyltransferase Mflv_1447 OS=Mycolicibacterium gilvu... [more]
Match NameE-valueIdentityDescription
A0A5D3CIS91.8e-293100.00Putative tRNA/rRNA methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BQ131.8e-293100.00uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 OS=Cucumis mel... [more]
A0A0A0LW979.4e-27995.45SpoU_sub_bind domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G5749... [more]
A0A6J1HPM25.0e-22478.74uncharacterized protein LOC111465489 OS=Cucurbita maxima OX=3661 GN=LOC111465489... [more]
A0A6J1FCE16.8e-22178.95rRNA methyltransferase 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
XP_008450781.10.0100.00PREDICTED: uncharacterized tRNA/rRNA methyltransferase MSMEG_6073/MSMEI_5913 [Cu... [more]
XP_004135672.10.095.45uncharacterized protein LOC101220884 [Cucumis sativus] >XP_031739747.1 uncharact... [more]
XP_038880007.13.55e-31285.56uncharacterized protein LOC120071698 [Benincasa hispida] >XP_038880008.1 unchara... [more]
KAG7021321.17.49e-28278.56rRNA methyltransferase 1, mitochondrial, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022965658.18.88e-28178.74uncharacterized protein LOC111465489 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G19870.11.0e-13153.50tRNA/rRNA methyltransferase (SpoU) family protein [more]
AT4G38020.11.7e-0624.71tRNA/rRNA methyltransferase (SpoU) family protein [more]
AT5G15390.13.2e-0520.99tRNA/rRNA methyltransferase (SpoU) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..171
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..124
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 79..181
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..177
NoneNo IPR availablePANTHERPTHR46103RRNA METHYLTRANSFERASE 1, MITOCHONDRIALcoord: 45..521
NoneNo IPR availableCDDcd18105SpoU-like_MRM1coord: 344..514
e-value: 1.15719E-73
score: 228.942
IPR013123RNA 2-O ribose methyltransferase, substrate bindingSMARTSM00967SpoU_sub_bind_2coord: 241..327
e-value: 1.2E-7
score: 41.4
IPR013123RNA 2-O ribose methyltransferase, substrate bindingPFAMPF08032SpoU_sub_bindcoord: 242..325
e-value: 1.2E-13
score: 51.1
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 326..526
e-value: 6.3E-47
score: 161.5
IPR004441RNA methyltransferase TrmH familyTIGRFAMTIGR00186TIGR00186coord: 271..513
e-value: 6.0E-51
score: 171.2
IPR02906450S ribosomal protein L30e-likeGENE3D3.30.1330.30coord: 235..323
e-value: 6.0E-17
score: 63.7
IPR02906450S ribosomal protein L30e-likeSUPERFAMILY55315L30e-likecoord: 241..324
IPR001537tRNA/rRNA methyltransferase, SpoU typePFAMPF00588SpoU_methylasecoord: 345..468
e-value: 3.4E-25
score: 88.9
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 320..515

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0013022.2IVF0013022.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001510 RNA methylation
biological_process GO:0006396 RNA processing
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003723 RNA binding
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0008168 methyltransferase activity