IVF0012875 (gene) Melon (IVF77) v1

Overview
NameIVF0012875
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description(S)-coclaurine N-methyltransferase-like
Locationchr07: 1327195 .. 1331426 (+)
RNA-Seq ExpressionIVF0012875
SyntenyIVF0012875
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGAAGATGCCGTTTTTGTCAGGCCACGCCACGTGGCCGAGCTCGGAGATAAAACGGTGGCTTAAAATGGAGAGACCAAAGAGTGGAGGTTAGCGTAATATTTGGGATCAAGCTTCACCCACGCAGAAATGGCGAAGCTTATTCAGATGCCCTACGACGTTTCTGTTCGTCTTGCATTAGCTTCTCTTGAACGGAATTTGTTGCCGGACGCCGTCGTAAGAACGTTCACCCGCTTGTTACTCGCTTCTCGTCTTCGCTCCGGTTACAAACCTTCCTCTCAACTTCAACTCTCCGAACTCCTCCATTTCGTTCACTGTAACTTCTTATACCTCTCCCTCTTTCTCTTTAAGTTATTAATGGAGATTCAACTAGTTATCAAATGATATTATGTTGCCCATGGTGGTTGTTTTTTTTACTATTTCTGCAGCTTTAGGGGAAATGCCCATAGCAATTAAGACTGATAAGCCAAAAGCTCAACACTACGAAGTACCGACCTCCTTCTTCAAGTTGGTTTTGGGGAAGAATCTCAAGTACAGGTAGTGAAATACTTGGAAATTCTTATTGCAGCTATTAGAAGAATTTACTTTCTATTTCATGCCACAGATATTCGTATAAGATGGATATGAATTTCTGATTTAATTATATGTAATGTGTACATGTATTGTAGATGTAGTGGTGCATGTTTATTGTATCATTGTAGTTTGATTTGAAATATGATATTGTAGTTCTGTATATGAGCAGAGGATGAATATTACTGTGATTTGTGAAGTTTTTCCCTCTTAAAAGTGGGCATTTAGGAGGTGTTTGGAGAGAGCTAGAATTAATCCGGATGAATGGAGATCAGATAAGTGTTGAAATGCACGGGATGGAAAAAAAATGAAATCGAAAAAAGTGTAAACGAAGAAAAATAGAGTTTAATTGAAAACTGTTGAAACGAAATAAGTTTAATATTATAATTCGGGTTTAAAATGTTCAATCTTAACATAAATTTTGGATTACATTATAAATGTATTTTAAAATTATTAGGGTATCCAAACACCATAAAGAGCGAGACTGATTTTGCTTGATTGGGGACTCTTATGGGCCCTTTTGATTTCTTTAATTCTCTCATAAAACGTTTGCTTTTTCTATTGATAAAACCTAGTGTGTATTGTTAGATAGATAAAACCTAGGTGATAAAGAGCTTCAACTCTCTTAATTCTAACAATTCATTATCCACTTGCATGATCTATTGCTGCAGTTGTTGCTATTTCAATGACAAATCAAGTACTTTAGAGGATGCTGAGGATGCAATGCTACAAATGTATTGTGAAAGGTCACAGTTGAAGGATGGTCACACTGTTCTTGATGTTGGATGTGGCTGGGGGTCGCTCTCATTGTACATTGCCCAAAAGTATAAGAATTGCACAGTAACAGGAATTTGCAATTCTATCACGCAGAAAGCTTACATCGAGGATCGGTGCCAGTAATTAGCTTTCTTCCATGTGCTTTGAATTTGATTTTAGATTTGTTAATTACTCAACACGAAGTTGGGTTTGGTGTCTTTTAATTTCTTCTAATTGATGTGTGGTTTCAGGGATCTCCAACTGCATAATGTCAACATCATAGTTGCAGACATAAGCACATATGAAATGGAAGCTGAATATGACAGAATATTTTCTATTGAAATGTTTGAGGTAACTAATTTCCTGTAGTAGTTTCATTTTTCTTAGGCAAATTGCGACGTAGTATAAGTTCTATTGGTTCTTACTCGAGCAGCATATGAAGAACTACAAGGATCTTCTCAAGAAAATATCAGGTTGGATGAAACAGGATAGCCTCCTATTTGTTCATTACTTTTGCCACAAGGTGTTTGCCTATCACTTTGAGGTATGCTTGATTCAGCTTGCTAGGGTCCTAAATTACTGGCTTCTTGATTCAATGCTGCTCACGTTTTATGTAGTAGAGATCTTTATAAACAGGAAATATTATGTATTTCTGACCATTTAATGGCCAAATTTATGCAGGATGTAAATGAAGATGATTGGATTACTAGATACTTCTTTGAAGGTGGTACAATGCCTTCCTCAAATCTACTTCTCTATTTCCAGGTGGATAAGCCTCCTTCCCCAGAACTATTATCTCTTATTCGTCTAGTTTTTTTAATTGTGCATATGGGTCGCACAAAATGAAAAGGGGGCATTTTGGCACAGTAGGTGGAGTTAATAAGTTTGGAGTTAATATATGTTGTAGGTGAATAAGTTTGTATTTGTGGTGTAGAGTTATAAGATGGAGTTTTTCGATATAAATATGAAAGGTAGAAAAAGAAGAAAGACGAAGTTGCGGAATAAATGTGAAAGTAAAGATAAAGAGGAAAATGAAGTTCCTTAATAAAGGTAAAAAGTAGAAAAGGAGAGGAAGATGAAGTTACACGATAAATACGAAAACTTAGTGTTTATTATTGAAATTGAGTTGTTTACACCAACTTAATAAGTTGGTGGGTGGTGGGTCAAACGTAGAGATGTCCAATTTTCCTAGGGGACCTAATTCTCCCAATTGGTTACGTTATTATTATATAAATAGTGCACTTAAATTTGTTTGATTGTTTATTGGCCAAGATAATGAGTGAGTTTAAATTCAAATTTTAAATTTAGACTATATATATATATGTGAATAAATTGATTTATGTTTATTTATAGAAACTAAAAATTATGTTTATTTATTTCTACTAAAAAAGGTTTGGTTTCTTTGATCAAAATTGATTAATTTATCCTTAAATTCAAATTGTGAGGTAAAACTAACAATAGTAAGCAACTTGGTAATATATTTAATTATTTAATTAGAATTTTGTTACCTTAATGATTCAAATTAATTGGTTTTTGTAAAAAAAGAAAAAAAAGAGACTAACAGAACGAATTTCCTGCGAGGAGTCCGATCCCCGCAAATTTTCCATGGGGGAATCCCTATTCTGATCTTATGGAAATTTCATGTTCTTATGGAAATTTCATGTCAATGGAGAATGAAATGGGAAGCGGGGATGGGGAATGACATCATCCCCGTGGACATTTTTAACCAAACAGCTTTGTGTTTGCTTGACTTTTCAAGTGTATGATTTAGAAAACAAGTCATTTTGAAAAAGAAAAAAATTAAGTATTCAACTCTTTGAACACTTTTAATTTTATTTCAAACATTTTTTATCAATAAGAGTTTAAATAATCATGATTCAGAAAACATTTTTTCTCTAATAAATTCAATTAGAGATAAAGTCTGTTTATAAGAATTTAATGTAGATTTCTCTAATGCTATTGCTAAGTGTTCGTATATCACATGTCATAACGGACAATGGATGTCCAAAATGTACAAATAGATCATTTATTCAAAAAACTATACTCGATTTTACTATTATTATTGAGAATTATTTCATCATTTCTTACATTTCATCAAAATATACCGACACTCTAACTTGGTACTATGACCAACTGACAATTTTATACAATAAAACCCACAAGAAGCTTTAACTGGAGATGTATCGTTTTTGCTTGTTGCATGCAGGAAGAGGTTTCAATTGTAGACCATTGGCATGTGAATGGCAAGCACTATTCTCAAACAAGGTAACACCGAACTATTTTAACAATTATCACGTATATACGTATTTGTAATTTTTTTTATAATCGTTACTATACACTTAATCGAAATTTCTACAAATGCTATTCAATGGAATTACCCTTTTAGAAATGTGATGAAAAGAATATTTAATATATCCTAATGCAGTGAAGAGTGGCTGAAAAGAATGGACAAGAACATAGCTTCAATAAAACCAATCATGGCAACTACTTATGGGAGGATTCGGCTGTGAAGTGGACTGTTTATTGGAGAACATTCTTTATTGCAGTGGCCGAGTTATTTGGTTACAACAATGGTGAAGAATGGATGGTTAGCCATTTCCTGTTCAAGAAGAAATGAATCAAACAACAAAGCTCCTGTGGTTTAAGAGTTCATAGAACAAGGTGCGATTTTTATGAGCTTGAATGAGTGAGTTTCTTCCTTTGATCTATTGATTTGGTGAAGCCATATGATCCAACATGTCCTGTTTATTGCAATATTTGTACTGTTAGTCAGATTAATTGGCATCGCGATGTGTCCCTTAAAGACCATTTTGCAAAACTTTTTAGCCTGTCATGGATATTATTAGAGGAAATTAGAATGCCGATTCGGGTATAATTTAATTAGTTTTGACCTTTGAACTTTAAAA

mRNA sequence

GAAGAAGATGCCGTTTTTGTCAGGCCACGCCACGTGGCCGAGCTCGGAGATAAAACGGTGGCTTAAAATGGAGAGACCAAAGAGTGGAGGTTAGCGTAATATTTGGGATCAAGCTTCACCCACGCAGAAATGGCGAAGCTTATTCAGATGCCCTACGACGTTTCTGTTCGTCTTGCATTAGCTTCTCTTGAACGGAATTTGTTGCCGGACGCCGTCGTAAGAACGTTCACCCGCTTGTTACTCGCTTCTCGTCTTCGCTCCGGTTACAAACCTTCCTCTCAACTTCAACTCTCCGAACTCCTCCATTTCGTTCACTCTTTAGGGGAAATGCCCATAGCAATTAAGACTGATAAGCCAAAAGCTCAACACTACGAAGTACCGACCTCCTTCTTCAAGTTGGTTTTGGGGAAGAATCTCAAGTACAGTTGTTGCTATTTCAATGACAAATCAAGTACTTTAGAGGATGCTGAGGATGCAATGCTACAAATGTATTGTGAAAGGTCACAGTTGAAGGATGGTCACACTGTTCTTGATGTTGGATGTGGCTGGGGGTCGCTCTCATTGTACATTGCCCAAAAGTATAAGAATTGCACAGTAACAGGAATTTGCAATTCTATCACGCAGAAAGCTTACATCGAGGATCGGTGCCAGGATCTCCAACTGCATAATGTCAACATCATAGTTGCAGACATAAGCACATATGAAATGGAAGCTGAATATGACAGAATATTTTCTATTGAAATGTTTGAGCATATGAAGAACTACAAGGATCTTCTCAAGAAAATATCAGGTTGGATGAAACAGGATAGCCTCCTATTTGTTCATTACTTTTGCCACAAGGTGTTTGCCTATCACTTTGAGGATGTAAATGAAGATGATTGGATTACTAGATACTTCTTTGAAGGTGGTACAATGCCTTCCTCAAATCTACTTCTCTATTTCCAGGAAGAGGTTTCAATTGTAGACCATTGGCATGTGAATGGCAAGCACTATTCTCAAACAAGTGAAGAGTGGCTGAAAAGAATGGACAAGAACATAGCTTCAATAAAACCAATCATGGCAACTACTTATGGGAGGATTCGGCTGTGAAGTGGACTGTTTATTGGAGAACATTCTTTATTGCAGTGGCCGAGTTATTTGGTTACAACAATGGTGAAGAATGGATGGTTAGCCATTTCCTGTTCAAGAAGAAATGAATCAAACAACAAAGCTCCTGTGGTTTAAGAGTTCATAGAACAAGGTGCGATTTTTATGAGCTTGAATGAGTGAGTTTCTTCCTTTGATCTATTGATTTGGTGAAGCCATATGATCCAACATGTCCTGTTTATTGCAATATTTGTACTGTTAGTCAGATTAATTGGCATCGCGATGTGTCCCTTAAAGACCATTTTGCAAAACTTTTTAGCCTGTCATGGATATTATTAGAGGAAATTAGAATGCCGATTCGGGTATAATTTAATTAGTTTTGACCTTTGAACTTTAAAA

Coding sequence (CDS)

ATGGCGAAGCTTATTCAGATGCCCTACGACGTTTCTGTTCGTCTTGCATTAGCTTCTCTTGAACGGAATTTGTTGCCGGACGCCGTCGTAAGAACGTTCACCCGCTTGTTACTCGCTTCTCGTCTTCGCTCCGGTTACAAACCTTCCTCTCAACTTCAACTCTCCGAACTCCTCCATTTCGTTCACTCTTTAGGGGAAATGCCCATAGCAATTAAGACTGATAAGCCAAAAGCTCAACACTACGAAGTACCGACCTCCTTCTTCAAGTTGGTTTTGGGGAAGAATCTCAAGTACAGTTGTTGCTATTTCAATGACAAATCAAGTACTTTAGAGGATGCTGAGGATGCAATGCTACAAATGTATTGTGAAAGGTCACAGTTGAAGGATGGTCACACTGTTCTTGATGTTGGATGTGGCTGGGGGTCGCTCTCATTGTACATTGCCCAAAAGTATAAGAATTGCACAGTAACAGGAATTTGCAATTCTATCACGCAGAAAGCTTACATCGAGGATCGGTGCCAGGATCTCCAACTGCATAATGTCAACATCATAGTTGCAGACATAAGCACATATGAAATGGAAGCTGAATATGACAGAATATTTTCTATTGAAATGTTTGAGCATATGAAGAACTACAAGGATCTTCTCAAGAAAATATCAGGTTGGATGAAACAGGATAGCCTCCTATTTGTTCATTACTTTTGCCACAAGGTGTTTGCCTATCACTTTGAGGATGTAAATGAAGATGATTGGATTACTAGATACTTCTTTGAAGGTGGTACAATGCCTTCCTCAAATCTACTTCTCTATTTCCAGGAAGAGGTTTCAATTGTAGACCATTGGCATGTGAATGGCAAGCACTATTCTCAAACAAGTGAAGAGTGGCTGAAAAGAATGGACAAGAACATAGCTTCAATAAAACCAATCATGGCAACTACTTATGGGAGGATTCGGCTGTGA

Protein sequence

MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPIMATTYGRIRL
Homology
BLAST of IVF0012875 vs. ExPASy Swiss-Prot
Match: Q5C9L6 ((S)-coclaurine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 7.0e-93
Identity = 156/304 (51.32%), Postives = 222/304 (73.03%), Query Frame = 0

Query: 17  LASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAIKTDKP 76
           L  LE  L+PD  ++   R+ L  RL+ G K + + Q+++L++  HSL +M IA + +  
Sbjct: 19  LKKLELGLVPDDEIKKLIRIQLGRRLQWGCKSTYEEQIAQLVNLTHSLRQMKIATEVETL 78

Query: 77  KAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGHTVLDV 136
             Q YEVP  F K++ G NLK SCCYF + S+TL++AE AML++YCER+Q+KDGH+VLD+
Sbjct: 79  DDQMYEVPIDFLKIMNGSNLKGSCCYFKNDSTTLDEAEIAMLELYCERAQIKDGHSVLDL 138

Query: 137 GCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTYEMEAE 196
           GCG G+L+LY+AQKYKN  VT + NS++QK +IE+  +   L NV +++ADI+T++M   
Sbjct: 139 GCGQGALTLYVAQKYKNSRVTAVTNSVSQKEFIEEESRKRNLSNVEVLLADITTHKMPDT 198

Query: 197 YDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDWITRYF 256
           YDRI  +E+FEHMKNY+ LL+KI  WM +D LLFV + CHK FAYH+E ++EDDW T Y 
Sbjct: 199 YDRILVVELFEHMKNYELLLRKIKEWMAKDGLLFVEHICHKTFAYHYEPIDEDDWFTEYV 258

Query: 257 FEGGTM--PSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPIMATTY 316
           F  GTM  PS++  LYFQ++VS+V+HW ++GKH+S+T+EEWLKR+D N+  IKP+  T  
Sbjct: 259 FPAGTMIIPSASFFLYFQDDVSVVNHWTLSGKHFSRTNEEWLKRLDANVELIKPMFVTIT 318

Query: 317 GRIR 319
           G+ R
Sbjct: 319 GQCR 322

BLAST of IVF0012875 vs. ExPASy Swiss-Prot
Match: Q7XB08 ((S)-coclaurine N-methyltransferase OS=Papaver somniferum OX=3469 GN=CNMT PE=1 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 2.2e-86
Identity = 150/296 (50.68%), Postives = 212/296 (71.62%), Query Frame = 0

Query: 17  LASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAIKTDKP 76
           L ++E  L+PD  +R   R+ L  RL+ GYK + + QLS+LL  VHSL  M +A + +  
Sbjct: 10  LRNMELGLIPDQEIRQLIRVELEKRLQWGYKETHEEQLSQLLDLVHSLKGMKMATEMENL 69

Query: 77  KAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGHTVLDV 136
             + YE P  F K+  G N+K S  Y+ D+S+TL++AE AML +Y ER+Q+KDG +VLD+
Sbjct: 70  DLKLYEAPMEFLKIQHGSNMKQSAGYYTDESTTLDEAEIAMLDLYMERAQIKDGQSVLDL 129

Query: 137 GCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTYEMEAE 196
           GCG G+++L+ A K+K C  TG+ +S+ QK YIE +C++L+L NV +++ADI+TYE E  
Sbjct: 130 GCGLGAVALFGANKFKKCQFTGVTSSVEQKDYIEGKCKELKLTNVKVLLADITTYETEER 189

Query: 197 YDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDWITRYF 256
           +DRIF++E+ EHMKNY+ LLKKIS WMK D LLFV + CHK  AYH+E V+ +DW T Y 
Sbjct: 190 FDRIFAVELIEHMKNYQLLLKKISEWMKDDGLLFVEHVCHKTLAYHYEPVDAEDWYTNYI 249

Query: 257 FEGG--TMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPIM 311
           F  G  T+ S+++LLYFQ++VS+V+ W ++GKHYS++ EEWLK MDKNI   K IM
Sbjct: 250 FPAGTLTLSSASMLLYFQDDVSVVNQWTLSGKHYSRSHEEWLKNMDKNIVEFKEIM 305

BLAST of IVF0012875 vs. ExPASy Swiss-Prot
Match: C3SBW0 (Pavine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.7e-84
Identity = 145/298 (48.66%), Postives = 211/298 (70.81%), Query Frame = 0

Query: 20  LERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAIKTDKPKAQ 79
           +E   + D  +R   ++ +  RL+ GYKP+ + QL++L+ F  SL  M +A + D   A+
Sbjct: 18  IEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQSLKGMEMAEEVDTLDAE 77

Query: 80  HYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGHTVLDVGCG 139
            YE+P  F  ++ GK LK+S  YF D+S+TL+++E  M+ +YCER+Q+KDG ++LD+GCG
Sbjct: 78  LYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLYCERAQIKDGQSILDLGCG 137

Query: 140 WGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTYEMEAEYDR 199
            GSL+L++AQKY+ C VTGI NS++QK +I D+C+ L L NV II+ D++ +E E  YDR
Sbjct: 138 HGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNVEIILEDVTKFETEITYDR 197

Query: 200 IFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDWITRYFFEG 259
           IF++ + EHMKNY+  LKK+S W+ Q  LLFV + CHKVFAY +E ++EDDW T Y F  
Sbjct: 198 IFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAYQYEPLDEDDWYTEYIFPS 257

Query: 260 GT--MPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPIMATTYG 316
           GT  M SS++LLYFQE+VS+V+HW ++GKH S   ++WLKR+D NI  +K I  + YG
Sbjct: 258 GTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRLDDNIDEVKEIFESFYG 315

BLAST of IVF0012875 vs. ExPASy Swiss-Prot
Match: C3SBU4 (Probable (S)-tetrahydroprotoberberine N-methyltransferase 2 OS=Papaver bracteatum OX=215227 PE=2 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 2.7e-76
Identity = 142/306 (46.41%), Postives = 204/306 (66.67%), Query Frame = 0

Query: 12  SVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAI 71
           S    L  L R  + D  ++   +  L  RL+ GYK S Q QLS  L F++SL +M ++ 
Sbjct: 11  SAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLKKMGMSG 70

Query: 72  KTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGH 131
           + +    + YE+PT  F+   GK++K S CYF  +SST+++AE+A  ++YCER+Q+KDG 
Sbjct: 71  QVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCERAQIKDGQ 130

Query: 132 TVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTY 191
           TVLD+GCG G L LY+AQKYKNC VTG+ NS  Q  YI  + + L L NV++I+AD++ Y
Sbjct: 131 TVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQY 190

Query: 192 EMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDW 251
           E +  YDRI  I + EHMKN +  +KK+S WM +DSLLFV + CHK F + FE ++EDDW
Sbjct: 191 ESDKTYDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDHSCHKTFNHFFEALDEDDW 250

Query: 252 ITRYFFEGG--TMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPI 311
            + Y F  G  T  S++ LLYFQ++VS+VDHW VNG H+++T + W K++DKN+ ++K I
Sbjct: 251 YSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAVKEI 310

Query: 312 MATTYG 316
           +    G
Sbjct: 311 LLPGLG 316

BLAST of IVF0012875 vs. ExPASy Swiss-Prot
Match: C3SBS8 ((S)-tetrahydroprotoberberine N-methyltransferase OS=Eschscholzia californica OX=3467 PE=1 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 3.0e-75
Identity = 134/301 (44.52%), Postives = 201/301 (66.78%), Query Frame = 0

Query: 17  LASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHFVHSLGEMPIAIKTDKP 76
           +  L +  + D  ++   R     R+  GYKP+ + QL+  L F+  L EM ++ + D  
Sbjct: 9   MGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSGEIDTM 68

Query: 77  KAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQMYCERSQLKDGHTVLDV 136
             + YE+PT+F + V GK +K SCCYF D++ST+++AE+A  ++YCER+Q+KDG TVLD+
Sbjct: 69  NKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQTVLDI 128

Query: 137 GCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHNVNIIVADISTYEMEAE 196
           GCG G L LYIA+KYKNC VTG+ NS  Q  YIE + + L+L NV++I AD++ ++ +  
Sbjct: 129 GCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKT 188

Query: 197 YDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFAYHFEDVNEDDWITRYF 256
           YDRI  +E  EHMKN +  +KK+S WM +DSLLFV +  HK F ++FE ++EDDW + + 
Sbjct: 189 YDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYSGFI 248

Query: 257 FEGG--TMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMDKNIASIKPIMATTY 316
           F  G  T+ SS+ LLYFQ++VS +DHW VNG H +++ E W K++D+ I + + I+    
Sbjct: 249 FPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILEPGL 308

BLAST of IVF0012875 vs. ExPASy TrEMBL
Match: A0A5A7VC26 ((S)-coclaurine N-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001960 PE=4 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 7.6e-183
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           KNIASIKPIMATTYG+
Sbjct: 301 KNIASIKPIMATTYGK 316

BLAST of IVF0012875 vs. ExPASy TrEMBL
Match: A0A0A0L426 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646310 PE=4 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 6.9e-176
Identity = 302/316 (95.57%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQ+PYD +V LALASLERNLLPDA++RTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQIPYDATVHLALASLERNLLPDAIIRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLREMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVN+DDWITRYFFEGGTM SSNLLLYFQ++VSIVDHW VNGKHYSQTSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRYFFEGGTMLSSNLLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           +NIASIKPIMATTYG+
Sbjct: 301 ENIASIKPIMATTYGK 316

BLAST of IVF0012875 vs. ExPASy TrEMBL
Match: A0A5A7VAA6 ((S)-coclaurine N-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G001950 PE=4 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 4.9e-174
Identity = 304/317 (95.90%), Postives = 308/317 (97.16%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQM YDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQMTYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLREMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAE-YDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVF 240
            NIIVAD+  YEME E YDRIFSI MFEHMKNYKDLLKKISGWMKQDSLLFVHYFCH+VF
Sbjct: 181 GNIIVADVRKYEMETEYYDRIFSIGMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHRVF 240

Query: 241 AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRM 300
           AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHW VNGKHYS+TSEEWLKRM
Sbjct: 241 AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWLVNGKHYSRTSEEWLKRM 300

Query: 301 DKNIASIKPIMATTYGR 317
           DKNIASIKPIMATTYG+
Sbjct: 301 DKNIASIKPIMATTYGK 317

BLAST of IVF0012875 vs. ExPASy TrEMBL
Match: A0A6J1CE09 ((S)-coclaurine N-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010387 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.5e-165
Identity = 282/316 (89.24%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MA+L+Q+PYDV+VRLALASLERNLLPDAVVR FTRLLLA+RLRSGYKPSSQLQLS+LLHF
Sbjct: 1   MAELMQLPYDVTVRLALASLERNLLPDAVVRRFTRLLLAARLRSGYKPSSQLQLSDLLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTD+PKAQHYE+PTSFF LVLGK+LKYSCCYFNDKSSTLEDAE+AMLQM
Sbjct: 61  VHSLREMPIAIKTDQPKAQHYELPTSFFNLVLGKHLKYSCCYFNDKSSTLEDAEEAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++ +DLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSATQKDYIEEQSRDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADIST+EMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFE++NEDDWITRYFFEGGTMPS+NLLLYFQ++VSIVDHW +NGKHYSQTSEEWLKRMD
Sbjct: 241 YHFENINEDDWITRYFFEGGTMPSANLLLYFQDDVSIVDHWLMNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           +NIA IKPIM TTYG+
Sbjct: 301 ENIALIKPIMETTYGK 316

BLAST of IVF0012875 vs. ExPASy TrEMBL
Match: A0A6J1FLU8 ((S)-coclaurine N-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446597 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 8.8e-163
Identity = 279/316 (88.29%), Postives = 301/316 (95.25%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MA+LIQ+PYDV+VRL +ASLERN LPDAVVR FTRLLLASR+RS +KPSSQLQLS+ LHF
Sbjct: 1   MAELIQVPYDVTVRLLMASLERNFLPDAVVRRFTRLLLASRIRSQFKPSSQLQLSDFLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VH+L EMPIAIKTDKPKAQHYE+PTSFFKLVLGKNLKYS CYF DKSSTLEDAE+AMLQM
Sbjct: 61  VHALREMPIAIKTDKPKAQHYELPTSFFKLVLGKNLKYSSCYFKDKSSTLEDAEEAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGH+VLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++C+DLQL+N
Sbjct: 121 YCERSQLKDGHSVLDVGCGWGSLSLYIAQNYKNCQVTGICNSTTQKDYIEEQCRDLQLNN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADIST+EMEAEYDRI SIEMFEHMKNYKDLLKKISGWMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIISIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFED NEDDWITRYFFEGGTMPS+NLLLYFQ++VSIVDHW VNG+HYSQTSEEWLKRMD
Sbjct: 241 YHFEDKNEDDWITRYFFEGGTMPSANLLLYFQDDVSIVDHWLVNGEHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           KNIASIKPIMATTYG+
Sbjct: 301 KNIASIKPIMATTYGK 316

BLAST of IVF0012875 vs. NCBI nr
Match: KAA0064517.1 ((S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa] >TYK20073.1 (S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 644 bits (1661), Expect = 1.52e-232
Identity = 315/316 (99.68%), Postives = 316/316 (100.00%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 316
           KNIASIKPIMATTYG+
Sbjct: 301 KNIASIKPIMATTYGK 316

BLAST of IVF0012875 vs. NCBI nr
Match: XP_004141332.1 ((S)-coclaurine N-methyltransferase [Cucumis sativus] >KGN55357.1 hypothetical protein Csa_011945 [Cucumis sativus])

HSP 1 Score: 621 bits (1601), Expect = 2.12e-223
Identity = 302/316 (95.57%), Postives = 311/316 (98.42%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQ+PYD +V LALASLERNLLPDA++RTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQIPYDATVHLALASLERNLLPDAIIRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLREMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA
Sbjct: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVN+DDWITRYFFEGGTM SSNLLLYFQ++VSIVDHW VNGKHYSQTSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRYFFEGGTMLSSNLLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 316
           +NIASIKPIMATTYG+
Sbjct: 301 ENIASIKPIMATTYGK 316

BLAST of IVF0012875 vs. NCBI nr
Match: KAA0064518.1 ((S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa] >TYK20072.1 (S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 615 bits (1585), Expect = 6.04e-221
Identity = 304/317 (95.90%), Postives = 308/317 (97.16%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MAKLIQM YDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAKLIQMTYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLREMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEY-DRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVF 240
            NIIVAD+  YEME EY DRIFSI MFEHMKNYKDLLKKISGWMKQDSLLFVHYFCH+VF
Sbjct: 181 GNIIVADVRKYEMETEYYDRIFSIGMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHRVF 240

Query: 241 AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRM 300
           AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHW VNGKHYS+TSEEWLKRM
Sbjct: 241 AYHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWLVNGKHYSRTSEEWLKRM 300

Query: 301 DKNIASIKPIMATTYGR 316
           DKNIASIKPIMATTYG+
Sbjct: 301 DKNIASIKPIMATTYGK 317

BLAST of IVF0012875 vs. NCBI nr
Match: XP_038898150.1 ((S)-coclaurine N-methyltransferase-like isoform X1 [Benincasa hispida] >XP_038898151.1 (S)-coclaurine N-methyltransferase-like isoform X1 [Benincasa hispida] >XP_038898152.1 (S)-coclaurine N-methyltransferase-like isoform X2 [Benincasa hispida])

HSP 1 Score: 608 bits (1568), Expect = 2.27e-218
Identity = 297/316 (93.99%), Postives = 307/316 (97.15%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MA+LIQMPYDV+VRLALASLERNLLPDAVVR FTRLLLASRLRSGYKPSSQLQLSELLHF
Sbjct: 1   MAELIQMPYDVTVRLALASLERNLLPDAVVRRFTRLLLASRLRSGYKPSSQLQLSELLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM
Sbjct: 61  VHSLKEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNC VTGICNS TQKAYIED+CQDLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCRVTGICNSTTQKAYIEDQCQDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADIST+EMEAEYDRIFSIEMFEHMKNYKDLLKKIS WMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISVWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVNEDDWITRYFFEGGTMPS+N+LLYFQ++VSIVDHW VNGKHYSQTSEEWLKRMD
Sbjct: 241 YHFEDVNEDDWITRYFFEGGTMPSANVLLYFQDDVSIVDHWLVNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 316
           KN  SIKPIMA TYG+
Sbjct: 301 KNADSIKPIMAATYGK 316

BLAST of IVF0012875 vs. NCBI nr
Match: XP_022139467.1 ((S)-coclaurine N-methyltransferase isoform X1 [Momordica charantia])

HSP 1 Score: 586 bits (1510), Expect = 1.56e-209
Identity = 282/316 (89.24%), Postives = 305/316 (96.52%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           MA+L+Q+PYDV+VRLALASLERNLLPDAVVR FTRLLLA+RLRSGYKPSSQLQLS+LLHF
Sbjct: 1   MAELMQLPYDVTVRLALASLERNLLPDAVVRRFTRLLLAARLRSGYKPSSQLQLSDLLHF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           VHSL EMPIAIKTD+PKAQHYE+PTSFF LVLGK+LKYSCCYFNDKSSTLEDAE+AMLQM
Sbjct: 61  VHSLREMPIAIKTDQPKAQHYELPTSFFNLVLGKHLKYSCCYFNDKSSTLEDAEEAMLQM 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCERSQLKDGHTVLDVGCGWGSLSLYIAQ YKNC VTGICNS TQK YIE++ +DLQLHN
Sbjct: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQNYKNCRVTGICNSATQKDYIEEQSRDLQLHN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           VNIIVADIST+EMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVH+FCHKVFA
Sbjct: 181 VNIIVADISTFEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHHFCHKVFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFE++NEDDWITRYFFEGGTMPS+NLLLYFQ++VSIVDHW +NGKHYSQTSEEWLKRMD
Sbjct: 241 YHFENINEDDWITRYFFEGGTMPSANLLLYFQDDVSIVDHWLMNGKHYSQTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 316
           +NIA IKPIM TTYG+
Sbjct: 301 ENIALIKPIMETTYGK 316

BLAST of IVF0012875 vs. TAIR 10
Match: AT4G33110.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-134
Identity = 219/316 (69.30%), Postives = 270/316 (85.44%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           M K+I + Y  SV++ L  LE NLLPD V+R  TRLLLA RLRSGYKP++++QLS+LL F
Sbjct: 1   MEKIIDVAYGASVKVGLTLLEMNLLPDIVIRRLTRLLLAGRLRSGYKPTAEMQLSDLLRF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           V S+ +MPIAI T+KPK QHYE+PT+FF+LVLG+N+KYS CYF++ SS+LEDAE+A+L +
Sbjct: 61  VDSIKKMPIAIHTEKPKTQHYELPTAFFELVLGRNMKYSSCYFSNDSSSLEDAEEAILAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCER++++DG +VLD+GCGWGSLSLYIA+KY  C +TGICNS TQKA+I+++C+ L L N
Sbjct: 121 YCERAKVEDGQSVLDIGCGWGSLSLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           + IIVADIST+E E  YDRIFSIEMFEHMKNY +LLKKI  WMK+DSLLFVHYFCHK FA
Sbjct: 181 IEIIVADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGKWMKEDSLLFVHYFCHKTFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVN+DDWITR+FF GGTMPS++LLLYFQE VSIVDHW V+G HY++TSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIVDHWLVSGTHYAKTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           K I ++K IM  TYG+
Sbjct: 301 KEIVAVKKIMEVTYGK 316

BLAST of IVF0012875 vs. TAIR 10
Match: AT4G33110.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 477.2 bits (1227), Expect = 1.0e-134
Identity = 219/316 (69.30%), Postives = 270/316 (85.44%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           M K+I + Y  SV++ L  LE NLLPD V+R  TRLLLA RLRSGYKP++++QLS+LL F
Sbjct: 1   MEKIIDVAYGASVKVGLTLLEMNLLPDIVIRRLTRLLLAGRLRSGYKPTAEMQLSDLLRF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           V S+ +MPIAI T+KPK QHYE+PT+FF+LVLG+N+KYS CYF++ SS+LEDAE+A+L +
Sbjct: 61  VDSIKKMPIAIHTEKPKTQHYELPTAFFELVLGRNMKYSSCYFSNDSSSLEDAEEAILAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           YCER++++DG +VLD+GCGWGSLSLYIA+KY  C +TGICNS TQKA+I+++C+ L L N
Sbjct: 121 YCERAKVEDGQSVLDIGCGWGSLSLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           + IIVADIST+E E  YDRIFSIEMFEHMKNY +LLKKI  WMK+DSLLFVHYFCHK FA
Sbjct: 181 IEIIVADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGKWMKEDSLLFVHYFCHKTFA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVN+DDWITR+FF GGTMPS++LLLYFQE VSIVDHW V+G HY++TSEEWLKRMD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIVDHWLVSGTHYAKTSEEWLKRMD 300

Query: 301 KNIASIKPIMATTYGR 317
           K I ++K IM  TYG+
Sbjct: 301 KEIVAVKKIMEVTYGK 316

BLAST of IVF0012875 vs. TAIR 10
Match: AT4G33120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 454.5 bits (1168), Expect = 6.9e-128
Identity = 209/316 (66.14%), Postives = 261/316 (82.59%), Query Frame = 0

Query: 1   MAKLIQMPYDVSVRLALASLERNLLPDAVVRTFTRLLLASRLRSGYKPSSQLQLSELLHF 60
           M K+I + Y  SV+  L  LE+NLLPD V+R   RLLLASRLRS YKP++++QLS+LL F
Sbjct: 1   MEKIIDVAYGASVKAGLTLLEKNLLPDLVIRRLIRLLLASRLRSVYKPTAEMQLSDLLRF 60

Query: 61  VHSLGEMPIAIKTDKPKAQHYEVPTSFFKLVLGKNLKYSCCYFNDKSSTLEDAEDAMLQM 120
           V SL +MPIAI T+  K QHYE+PT+FF+  LG+N+KYSCCY+++ SS+LE+A +AML +
Sbjct: 61  VDSLKKMPIAINTETAKTQHYELPTAFFEHALGRNIKYSCCYYSNDSSSLEEAGEAMLAL 120

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
            CER++++DG +VLD+GCGWGSL+LYIA+KY  C +TGICNS TQKA+I+++C+ L L N
Sbjct: 121 SCERAKVEDGQSVLDIGCGWGSLTLYIARKYSKCKLTGICNSKTQKAFIDEKCRKLGLQN 180

Query: 181 VNIIVADISTYEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFCHKVFA 240
           V II ADIST+E E  YDRIFSIEMFEHMKNY +LLKKI  WMK+DSLLFVHY CHK +A
Sbjct: 181 VEIIAADISTFEHEGTYDRIFSIEMFEHMKNYGELLKKIGNWMKEDSLLFVHYLCHKTYA 240

Query: 241 YHFEDVNEDDWITRYFFEGGTMPSSNLLLYFQEEVSIVDHWHVNGKHYSQTSEEWLKRMD 300
           YHFEDVN+DDWITR+FF GGTMPS++LLLYFQE VSI+DHW VNG HY++TSEEWLK MD
Sbjct: 241 YHFEDVNDDDWITRHFFSGGTMPSADLLLYFQENVSIMDHWLVNGTHYAKTSEEWLKGMD 300

Query: 301 KNIASIKPIMATTYGR 317
           K I ++K IM  TYG+
Sbjct: 301 KEIVAVKEIMEVTYGK 316

BLAST of IVF0012875 vs. TAIR 10
Match: AT3G18000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 6.1e-07
Identity = 34/116 (29.31%), Postives = 59/116 (50.86%), Query Frame = 0

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           + E+  LK G  VLDVGCG G    Y+A+K+ +  V GI  S+   ++  +R   L   +
Sbjct: 274 FVEKMNLKPGQKVLDVGCGIGGGDFYMAEKF-DVHVVGIDLSVNMISFALERAIGLSC-S 333

Query: 181 VNIIVADIST-YEMEAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFC 236
           V   VAD +T +  +  +D I+S +   H+++   L +    W+K    + +  +C
Sbjct: 334 VEFEVADCTTKHYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLISDYC 387

BLAST of IVF0012875 vs. TAIR 10
Match: AT1G48600.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 2.0e-05
Identity = 32/116 (27.59%), Postives = 57/116 (49.14%), Query Frame = 0

Query: 121 YCERSQLKDGHTVLDVGCGWGSLSLYIAQKYKNCTVTGICNSITQKAYIEDRCQDLQLHN 180
           +  +  LK G  VLDVGCG G    Y+A+ + +  V GI  S+   ++  +R   L+  +
Sbjct: 274 FVAKMDLKPGQKVLDVGCGIGGGDFYMAENF-DVHVVGIDLSVNMISFALERAIGLKC-S 333

Query: 181 VNIIVADISTYEM-EAEYDRIFSIEMFEHMKNYKDLLKKISGWMKQDSLLFVHYFC 236
           V   VAD +T    +  +D I+S +   H+++   L +    W+K    + +  +C
Sbjct: 334 VEFEVADCTTKTYPDNSFDVIYSRDTILHIQDKPALFRTFFKWLKPGGKVLITDYC 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5C9L67.0e-9351.32(S)-coclaurine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095... [more]
Q7XB082.2e-8650.68(S)-coclaurine N-methyltransferase OS=Papaver somniferum OX=3469 GN=CNMT PE=1 SV... [more]
C3SBW02.7e-8448.66Pavine N-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV... [more]
C3SBU42.7e-7646.41Probable (S)-tetrahydroprotoberberine N-methyltransferase 2 OS=Papaver bracteatu... [more]
C3SBS83.0e-7544.52(S)-tetrahydroprotoberberine N-methyltransferase OS=Eschscholzia californica OX=... [more]
Match NameE-valueIdentityDescription
A0A5A7VC267.6e-18399.68(S)-coclaurine N-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0L4266.9e-17695.57Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G646310 PE=4 SV=1[more]
A0A5A7VAA64.9e-17495.90(S)-coclaurine N-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1CE092.5e-16589.24(S)-coclaurine N-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1FLU88.8e-16388.29(S)-coclaurine N-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446597... [more]
Match NameE-valueIdentityDescription
KAA0064517.11.52e-23299.68(S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa] >TYK20073.1 (... [more]
XP_004141332.12.12e-22395.57(S)-coclaurine N-methyltransferase [Cucumis sativus] >KGN55357.1 hypothetical pr... [more]
KAA0064518.16.04e-22195.90(S)-coclaurine N-methyltransferase-like [Cucumis melo var. makuwa] >TYK20072.1 (... [more]
XP_038898150.12.27e-21893.99(S)-coclaurine N-methyltransferase-like isoform X1 [Benincasa hispida] >XP_03889... [more]
XP_022139467.11.56e-20989.24(S)-coclaurine N-methyltransferase isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT4G33110.11.0e-13469.30S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G33110.21.0e-13469.30S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G33120.16.9e-12866.14S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G18000.16.1e-0729.31S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G48600.22.0e-0527.59S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF02353CMAScoord: 77..309
e-value: 8.2E-45
score: 153.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 71..316
e-value: 3.8E-74
score: 251.4
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 122..250
e-value: 6.2E-4
score: 17.1
NoneNo IPR availablePANTHERPTHR43832FAMILY NOT NAMEDcoord: 5..317
NoneNo IPR availablePANTHERPTHR43832:SF1S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEINcoord: 5..317
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 132..234
e-value: 3.94367E-9
score: 51.6619
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 79..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0012875.2IVF0012875.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity