IVF0012623 (gene) Melon (IVF77) v1

Overview
NameIVF0012623
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
Locationchr02: 721165 .. 722585 (+)
RNA-Seq ExpressionIVF0012623
SyntenyIVF0012623
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAGCTGTTCCACACCAAAGGCAGGCTAGGAAGAACAATGGGTTGCCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGACGACGTTTGCCGATACTTCTACACCACGACGAGGCACGTCTACGAAGAAACCTTCGATGCAAAAGCGTTATCCAACCGAGTTGAAGAAGTTTTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATCCGCCGTTGAAGAAATGGAGAAGAATAAGCAAGTGTTGAACAATGGGAACATCAGAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTGATAAAAATGGTCAAAATTCTTCTACTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTACAAGAAAGAATTGGAAGATTATATGGATTATGATATTGGTTCATATTGTAAAGATGATTGGAATCTTGCTCAGAAACTTATGCTCAATGGTTGCGATCCATTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGACGCCGGACGATCGGAACGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTGTCTAGCAAAAATCCCAAAGGGGATACAACAAATGTAGTGGTTGTTTTGAAATAGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCTGTCGATTTTGCAATATCGGATGTTCTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTCGGGATCAGCACGGGGAGTTTTGCGGCGAGAATGAGAGAAGAGAATGTGACAATTGTGACTACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCTTTGTATTCGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAAGTGGTTTCATGGATGGTTGGCTAGATATGTTGTTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGGCCGGGAGGATTGTTATGGATCGACCGATTCTTTTGTGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

mRNA sequence

ATGAAGAAGCTGTTCCACACCAAAGGCAGGCTAGGAAGAACAATGGGTTGCCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGACGACGTTTGCCGATACTTCTACACCACGACGAGGCACGTCTACGAAGAAACCTTCGATGCAAAAGCGTTATCCAACCGAGTTGAAGAAGTTTTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATCCGCCGTTGAAGAAATGGAGAAGAATAAGCAAGTGTTGAACAATGGGAACATCAGAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTGATAAAAATGGTCAAAATTCTTCTACTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTACAAGAAAGAATTGGAAGATTATATGGATTATGATATTGGTTCATATTGTAAAGATGATTGGAATCTTGCTCAGAAACTTATGCTCAATGGTTGCGATCCATTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGACGCCGGACGATCGGAACGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTTGGTTGTTTTGAAATAGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCTGTCGATTTTGCAATATCGGATGTTCTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTCGGGATCAGCACGGGGAGTTTTGCGGCGAGAATGAGAGAAGAGAATGTGACAATTGTGACTACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCTTTGTATTCGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAAGTGGTTTCATGGATGGTTGGCTAGATATGTTGTTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGGCCGGGAGGATTGTTATGGATCGACCGATTCTTTTGTGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

Coding sequence (CDS)

ATGAAGAAGCTGTTCCACACCAAAGGCAGGCTAGGAAGAACAATGGGTTGCCTTCAAATTCTATTAATAGGACTTGTTATCATCATGACCACATTGAGTCTCTTCAAGTTCTACTCAGCTGGATTTATCTTTCACAAGGACGACGTTTGCCGATACTTCTACACCACGACGAGGCACGTCTACGAAGAAACCTTCGATGCAAAAGCGTTATCCAACCGAGTTGAAGAAGTTTTAGATCAGCTAGAAAGTTTACAAGAGAAACTCGAATCCGCCGTTGAAGAAATGGAGAAGAATAAGCAAGTGTTGAACAATGGGAACATCAGAATGATTGAGCATAAAAAGTACTTAGAAGATGAAGTGATTAAGCCTCTTTATAATGCTCATATTGCTTTAAGGCAAATAAGATTACCAAAAGTTGATAAAAATGGTCAAAATTCTTCTACTATGAAAGAAGAGCCTTTGATCAACAATTTTGTGGTTGAGGAAATAAGAAAATATATTTCTCCAAAGAAAAGTAGAATTGGGAAACTCAATATGTATATGACTGAGAATATATACAACACAATTGGACATGCTTGTGTTCTGTACAAGAAAGAATTGGAAGATTATATGGATTATGATATTGGTTCATATTGTAAAGATGATTGGAATCTTGCTCAGAAACTTATGCTCAATGGTTGCGATCCATTGCCTCGTCGACGGTGCTTGACAAGAGCGTCGAAGGTCTACCAAAAGCCGTATCCATTGAACGAATCGCTATGGAAGACGCCGGACGATCGGAACGTAAGATGGAGTAACTATCAATGTAGGAACTTTTCTTGCTTTGGTTGTTTTGAAATAGAGAAAGAGAAGCTCAAATGGGTCATTAGCAATAGCTCTCTCGCTGTCGATTTTGCAATATCGGATGTTCTAGCGATCAAGCCGGGGGAGATTCGGATCGGGTTGGATTTCGGGATCAGCACGGGGAGTTTTGCGGCGAGAATGAGAGAAGAGAATGTGACAATTGTGACTACAGCTTTGAACCTTGGAGCTCCTTTCAATGAATTTATTGCTTTAAGAGGTTTGTTTCCTTTGTATTCGACATTAAACCAAAGACTTCCTCTGTTTGATAACACAATGGATTTGATTCACACAAGTGGTTTCATGGATGGTTGGCTAGATATGTTGTTGTTGGATTTCATACTTTTCGATTGGGATCGTGTTCTACGGCCGGGAGGATTGTTATGGATCGACCGATTCTTTTGTGATCGAAAGGATCTCGACGATTACATGTACATGTTTCTGCAGTTTAGATACAAGAAACATAAATGGAGTATTGCTCCTAAATCAAAAAATGAAGTCTACCTTTCTGCATTGTTGGAAAAACCTCCAAGGGCAATATGA

Protein sequence

MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCFGCFEIEKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI
Homology
BLAST of IVF0012623 vs. ExPASy Swiss-Prot
Match: Q8RWB7 (Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE=2 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 9.3e-54
Identity = 125/352 (35.51%), Postives = 189/352 (53.69%), Query Frame = 0

Query: 118 DEVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKL 177
           D +++ L+    A+  +   + DK+   S++               R  +S   S     
Sbjct: 50  DHLLRQLHTIRAAINHLTTHQPDKSTSTSTS---------------RAAVSSSSSSTAPK 109

Query: 178 NMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLT 237
            + +   + + I  AC  Y   L +YM+Y   S C  D +L +KL+L GC PLPRRRC +
Sbjct: 110 ELLIYSKL-SPIASACHNYPDLLHEYMNYTPFSLCPSDTDLVEKLILRGCHPLPRRRCFS 169

Query: 238 RASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCFGC------FEIEKEKLKWVISN 297
           R         P N S   +  + NV WS Y C++F C         F++  EK K   S 
Sbjct: 170 RT--------PRNPS--DSKPESNVLWSYYSCKSFDCLITKFSDLGFDLSLEKSKSQFSA 229

Query: 298 SSLAVDFAISDVLAIKPGE---IRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNE 357
               +D  IS +L I       +R+G+D G  TGSFAA M+  NVT++TT +N  AP++E
Sbjct: 230 YKSELDLPISQLLQIAKSANSVLRLGIDVGGGTGSFAAAMKARNVTVLTTTMNFNAPYSE 289

Query: 358 FIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGLL 417
            +A+RGL PL+  L QRLP+FD  +DL+     ++ W+ + +++F  FD DR+LR GG L
Sbjct: 290 AVAMRGLVPLHVPLQQRLPVFDGVVDLVRCGRAVNRWIPVTVMEFFFFDLDRILRGGGYL 349

Query: 418 WIDRFFCDRKDLDD-YMYMFLQFRYKKHKWSIAPKSKN---EVYLSALLEKP 457
           W+DRFF  + DL++ Y  M  +  YKK KW++A K+ +   EV+L+ALL+KP
Sbjct: 350 WLDRFFSKKVDLENVYAPMIGKLGYKKVKWAVANKADSKHGEVFLTALLQKP 375

BLAST of IVF0012623 vs. ExPASy TrEMBL
Match: A0A5D3CEF4 (Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003900 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.3e-265
Identity = 459/473 (97.04%), Postives = 459/473 (97.04%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSC              GCFEIEKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of IVF0012623 vs. ExPASy TrEMBL
Match: A0A1S3BQC0 (uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.3e-265
Identity = 459/473 (97.04%), Postives = 459/473 (97.04%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSC              GCFEIEKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of IVF0012623 vs. ExPASy TrEMBL
Match: A0A6J1CY71 (uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015349 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.5e-232
Identity = 402/473 (84.99%), Postives = 433/473 (91.54%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           M+KL HTK RLGRTMG LQILL GLVI++TTLSLFKFYSAGF FHK+DVCRYFY TT+HV
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFY-TTKHV 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YE +FD KALS+RVEEVLDQL+ LQ KLESAVEEMEKNKQVL+NG+I  +EHKKYLE+EV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKV+K    +S M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTE IYNTIGHACVL++KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQ+PYP+NESLWK PDDRNVRWSNYQCRNFSC              GCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           V +NSSL+VDF +SDVLA+K GEIR+GLDFGIS+GSFAARM+E+NVTIVTTALNLGAPFN
Sbjct: 301 V-TNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of IVF0012623 vs. ExPASy TrEMBL
Match: A0A6J1I1D3 (uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555 PE=3 SV=1)

HSP 1 Score: 810.8 bits (2093), Expect = 2.9e-231
Identity = 401/475 (84.42%), Postives = 433/475 (91.16%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFY--TTTR 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFY  TTT+
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  HVYEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLED 120
            +YE +FDAKALS+RVEEVLDQLESLQ+KLES VEEME++KQ L+NGN+R IEHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLN 180
           EVIKPLYNAHI LRQIRLPKV+K  +NSS MKEEPLINNFVVEEIRKYI+PKKSR GK+N
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSS-MKEEPLINNFVVEEIRKYITPKKSRTGKVN 180

Query: 181 MYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTR 240
           MYMTE+IYNTIGHACVL +KELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCLTR
Sbjct: 181 MYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTR 240

Query: 241 ASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKL 300
           ASKVYQKPYP+NESLWKTPDDRNVRWSNYQCRNFSC              GCFE+EKEK+
Sbjct: 241 ASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKV 300

Query: 301 KWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAP 360
           KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNLGAP
Sbjct: 301 KWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAP 360

Query: 361 FNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPG 420
           FNEFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGW+DMLLLDFILFDWDRVLRPG
Sbjct: 361 FNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPG 420

Query: 421 GLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
           GLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 GLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of IVF0012623 vs. ExPASy TrEMBL
Match: A0A6J1EZZ0 (uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC111440913 PE=3 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.0e-229
Identity = 401/478 (83.89%), Postives = 433/478 (90.59%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFY-----T 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFY     T
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTPPTTT 60

Query: 61  TTRHVYEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKY 120
           TT+ VYE +FDAKALS+RVEEVLDQLESLQ+KLESAVEEME++KQ L+NGN+R IEHKKY
Sbjct: 61  TTKQVYEASFDAKALSDRVEEVLDQLESLQDKLESAVEEMERDKQALSNGNMRRIEHKKY 120

Query: 121 LEDEVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIG 180
           LE EVIKPLYNAHIALRQIRLPKV+K  +N S +KEEPLINNFVVEEIRKYI+PKKSR G
Sbjct: 121 LEMEVIKPLYNAHIALRQIRLPKVEKVKENLS-VKEEPLINNFVVEEIRKYITPKKSRTG 180

Query: 181 KLNMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRC 240
           K+NMYMTE+IYNTIGHACVL +KELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRC
Sbjct: 181 KVNMYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNVGQKLMLNGCDPLPRRRC 240

Query: 241 LTRASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEK 300
           LTRASKVYQKPY +NESLWKTPDDRNVRWSNYQCRNFSC              GCFE+EK
Sbjct: 241 LTRASKVYQKPYAVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEK 300

Query: 301 EKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNL 360
           EK+KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNL
Sbjct: 301 EKVKWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNL 360

Query: 361 GAPFNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVL 420
           GAPFNEFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGW+DMLLLDFILFDWDRVL
Sbjct: 361 GAPFNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVL 420

Query: 421 RPGGLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
           RPGGLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 RPGGLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 476

BLAST of IVF0012623 vs. NCBI nr
Match: XP_008450643.1 (PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 putative Methyltransferase [Cucumis melo var. makuwa] >TYK10243.1 putative Methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 921 bits (2381), Expect = 0.0
Identity = 459/473 (97.04%), Postives = 459/473 (97.04%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV
Sbjct: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSC              GCFEIEKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 460
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of IVF0012623 vs. NCBI nr
Match: XP_011659893.1 (uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetical protein Csa_006971 [Cucumis sativus])

HSP 1 Score: 893 bits (2307), Expect = 0.0
Identity = 445/473 (94.08%), Postives = 449/473 (94.93%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           MKKLFHTKGRLGRTMG LQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRH 
Sbjct: 1   MKKLFHTKGRLGRTMGWLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHA 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YEETFDAKALSNRVEEVLDQLESLQEKLE  VEEM KNKQVLNNG I MIEHKKYLEDEV
Sbjct: 61  YEETFDAKALSNRVEEVLDQLESLQEKLEFTVEEMGKNKQVLNNGKITMIEHKKYLEDEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           IKPLYNAHIALRQIRLPKV+KNGQNSS MKEEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IKPLYNAHIALRQIRLPKVEKNGQNSSAMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTENIYNTIGHACVLY+KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS
Sbjct: 181 MTENIYNTIGHACVLYRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQKPYPLNESLWK PDDRNVRWSNYQCRNFSC              GCFE+EKEKLKW
Sbjct: 241 KVYQKPYPLNESLWKMPDDRNVRWSNYQCRNFSCLSSNNPKKGYNKCSGCFEMEKEKLKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN
Sbjct: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGLFPLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLFPLYLTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 460
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 473

BLAST of IVF0012623 vs. NCBI nr
Match: XP_038880508.1 (uncharacterized protein LOC120072172 [Benincasa hispida])

HSP 1 Score: 858 bits (2216), Expect = 8.26e-313
Identity = 428/468 (91.45%), Postives = 442/468 (94.44%), Query Frame = 0

Query: 6   HTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETF 65
             KGRLGRTMG LQILL GLVII+TTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEE+F
Sbjct: 2   RNKGRLGRTMGWLQILLGGLVIIITTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEESF 61

Query: 66  DAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEVIKPLY 125
           DAKALS+RVEEVLDQLESLQEKLESAVEEM KNKQVLNNGNI  IEHKKYLE+EVIKPLY
Sbjct: 62  DAKALSDRVEEVLDQLESLQEKLESAVEEMGKNKQVLNNGNITRIEHKKYLEEEVIKPLY 121

Query: 126 NAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI 185
           NAHIALRQIRLPKV+KNGQN ST+KEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI
Sbjct: 122 NAHIALRQIRLPKVEKNGQNYSTVKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENI 181

Query: 186 YNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK 245
           YNTIGHACVL++KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK
Sbjct: 182 YNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQK 241

Query: 246 PYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKWVISNS 305
           PYP+NESLWK+PDDRNVRWSNYQCRNFSC              GCFEI+KEKLKWVI NS
Sbjct: 242 PYPVNESLWKSPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEIDKEKLKWVI-NS 301

Query: 306 SLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIAL 365
           SLAVDF ISDVLAIKPGEIRIGLDFGISTGSFAARMRE+NVTIVTTALNLGAPFNEFIAL
Sbjct: 302 SLAVDFTISDVLAIKPGEIRIGLDFGISTGSFAARMREQNVTIVTTALNLGAPFNEFIAL 361

Query: 366 RGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 425
           RGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR
Sbjct: 362 RGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDR 421

Query: 426 FFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 460
           FFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 422 FFCDRKDLDDYMYMFLQFRYKKHKWTIAPKSKNEVYLSALLEKPPRAI 468

BLAST of IVF0012623 vs. NCBI nr
Match: XP_022146046.1 (uncharacterized protein LOC111015349 [Momordica charantia])

HSP 1 Score: 812 bits (2098), Expect = 8.78e-295
Identity = 402/473 (84.99%), Postives = 433/473 (91.54%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHV 60
           M+KL HTK RLGRTMG LQILL GLVI++TTLSLFKFYSAGF FHK+DVCRYFYTT +HV
Sbjct: 1   MRKLIHTKCRLGRTMGWLQILLGGLVILITTLSLFKFYSAGFFFHKEDVCRYFYTT-KHV 60

Query: 61  YEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEV 120
           YE +FD KALS+RVEEVLDQL+ LQ KLESAVEEMEKNKQVL+NG+I  +EHKKYLE+EV
Sbjct: 61  YEASFDVKALSDRVEEVLDQLDGLQAKLESAVEEMEKNKQVLSNGSITRLEHKKYLEEEV 120

Query: 121 IKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180
           I+PLYNAHIALRQIRLPKV+K    +S M+EEPLINNFVVEEIRKYISPKKSRIGKLNMY
Sbjct: 121 IRPLYNAHIALRQIRLPKVEKIDDQNSNMQEEPLINNFVVEEIRKYISPKKSRIGKLNMY 180

Query: 181 MTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRAS 240
           MTE IYNTIGHACVL++KELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCL RAS
Sbjct: 181 MTEKIYNTIGHACVLHRKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLARAS 240

Query: 241 KVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKW 300
           KVYQ+PYP+NESLWK PDDRNVRWSNYQCRNFSC              GCFE+EKEK KW
Sbjct: 241 KVYQRPYPVNESLWKLPDDRNVRWSNYQCRNFSCLSSKNPRRGYNKCSGCFELEKEKRKW 300

Query: 301 VISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFN 360
           V +NSSL+VDF +SDVLA+K GEIR+GLDFGIS+GSFAARM+E+NVTIVTTALNLGAPFN
Sbjct: 301 V-TNSSLSVDFVLSDVLALKAGEIRVGLDFGISSGSFAARMKEQNVTIVTTALNLGAPFN 360

Query: 361 EFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGL 420
           EFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGWLDMLLLDFILFDWDRVLRPGGL
Sbjct: 361 EFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWLDMLLLDFILFDWDRVLRPGGL 420

Query: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 460
           LWIDRFFCDRKDLDDYMYMFLQFRYKKHKW+IAPKSKNEVYLSALLEKPPRAI
Sbjct: 421 LWIDRFFCDRKDLDDYMYMFLQFRYKKHKWAIAPKSKNEVYLSALLEKPPRAI 471

BLAST of IVF0012623 vs. NCBI nr
Match: XP_022969573.1 (uncharacterized protein LOC111468555 [Cucurbita maxima])

HSP 1 Score: 808 bits (2087), Expect = 4.48e-293
Identity = 401/475 (84.42%), Postives = 433/475 (91.16%), Query Frame = 0

Query: 1   MKKLFHTKGRLGRTMGCLQILLIGLVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTT--R 60
           M+KL HTK R+GRTMGCLQILL GLVI +TTLSLFKFYSAGFIFHK+DVCRYFYTTT  +
Sbjct: 1   MRKLIHTKWRVGRTMGCLQILLGGLVITITTLSLFKFYSAGFIFHKEDVCRYFYTTTTTK 60

Query: 61  HVYEETFDAKALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLED 120
            +YE +FDAKALS+RVEEVLDQLESLQ+KLES VEEME++KQ L+NGN+R IEHKKYLE 
Sbjct: 61  QIYEASFDAKALSDRVEEVLDQLESLQDKLESVVEEMERDKQALSNGNMRRIEHKKYLEM 120

Query: 121 EVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLN 180
           EVIKPLYNAHI LRQIRLPKV+K  +NSS MKEEPLINNFVVEEIRKYI+PKKSR GK+N
Sbjct: 121 EVIKPLYNAHIGLRQIRLPKVEKMNENSS-MKEEPLINNFVVEEIRKYITPKKSRTGKVN 180

Query: 181 MYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTR 240
           MYMTE+IYNTIGHACVL +KELE+YM YDIGSYCKDDWN+ QKLMLNGCDPLPRRRCLTR
Sbjct: 181 MYMTESIYNTIGHACVLVRKELEEYMSYDIGSYCKDDWNMGQKLMLNGCDPLPRRRCLTR 240

Query: 241 ASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKL 300
           ASKVYQKPYP+NESLWKTPDDRNVRWSNYQCRNFSC              GCFE+EKEK+
Sbjct: 241 ASKVYQKPYPVNESLWKTPDDRNVRWSNYQCRNFSCLSSKNPKRGYNKCSGCFEMEKEKV 300

Query: 301 KWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAP 360
           KWV  NSS+AVDF ISDVL +K GEIR+GLDFGISTGSF ARMREENVTIVTTALNLGAP
Sbjct: 301 KWV-RNSSVAVDFRISDVLGMKAGEIRVGLDFGISTGSFGARMREENVTIVTTALNLGAP 360

Query: 361 FNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPG 420
           FNEFIALRGL PLY TLNQRLPLFDNTMDLIHT+GFMDGW+DMLLLDFILFDWDRVLRPG
Sbjct: 361 FNEFIALRGLIPLYVTLNQRLPLFDNTMDLIHTTGFMDGWVDMLLLDFILFDWDRVLRPG 420

Query: 421 GLLWIDRFFCDRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 460
           GLLWIDRFFCDRKDLDDY+YMFLQFRYKKHKW+IAPKSKNEVYLSA+LEKPPRAI
Sbjct: 421 GLLWIDRFFCDRKDLDDYIYMFLQFRYKKHKWAIAPKSKNEVYLSAVLEKPPRAI 473

BLAST of IVF0012623 vs. TAIR 10
Match: AT3G05390.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 15 plant structures; EXPRESSED DURING: 7 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF248, methyltransferase putative (InterPro:IPR004159); BEST Arabidopsis thaliana protein match is: S-adenosyl-L-methionine-dependent methyltransferases superfamily protein (TAIR:AT4G01240.1); Has 507 Blast hits to 498 proteins in 33 species: Archae - 4; Bacteria - 8; Metazoa - 0; Fungi - 0; Plants - 493; Viruses - 0; Other Eukaryotes - 2 (source: NCBI BLink). )

HSP 1 Score: 646.7 bits (1667), Expect = 1.4e-185
Identity = 320/465 (68.82%), Postives = 380/465 (81.72%), Query Frame = 0

Query: 10  RLGRTMGCLQILLIGLVIIMTTLS-LFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDAK 69
           RLGR M  LQ++L GL++I  +LS L++F+S G+    +D C   Y T + V  E FD K
Sbjct: 10  RLGRLMAGLQVIL-GLLVITVSLSCLYRFHSVGYFLQNEDTCNNIY-TIKEVSSEGFDLK 69

Query: 70  ALSNRVEEVLDQLESLQEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEVIKPLYNAH 129
           AL +RV+EVL+++++L EKLE  V++MEK+K    +G+ +  E KK+LEDEV+KP Y AH
Sbjct: 70  ALHDRVDEVLEKMDNLYEKLEKTVKDMEKSK----DGSKK--EMKKFLEDEVMKPFYYAH 129

Query: 130 IALRQIRLPKVDKNGQNSSTMKEEPLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNT 189
           I LRQIRLPK +  G  +ST KEEPLIN F++EEIR+YI+PK++R+GK+NM+ TE +YNT
Sbjct: 130 IGLRQIRLPKPE--GIRNSTEKEEPLINKFLIEEIRQYITPKENRVGKINMFGTERVYNT 189

Query: 190 IGHACVLYKKELEDYMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYP 249
           IGHAC L K ELE YMDYD+G+YC DDWNLAQKLMLNGCDPLPRRRCLTRAS  YQKPYP
Sbjct: 190 IGHACALMKMELEKYMDYDVGAYCDDDWNLAQKLMLNGCDPLPRRRCLTRASMTYQKPYP 249

Query: 250 LNESLWKTPDDRNVRWSNYQCRNFSCF-------------GCFEIEKEKLKWVISNSSLA 309
           +NESLWK PDDRNVRW NYQCRNF+C              GCFE+EKEK KWV  NSSL 
Sbjct: 250 INESLWKLPDDRNVRWGNYQCRNFACLSSKNPKRGYTKCSGCFEMEKEKDKWV-KNSSLL 309

Query: 310 VDFAISDVLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGL 369
           VDF I DVL +KP EIRIGLD+G+ TG+FAARMRE+NVTIVTTALNLGAPFNE IALRGL
Sbjct: 310 VDFMIEDVLRVKPSEIRIGLDYGVGTGTFAARMREKNVTIVTTALNLGAPFNEMIALRGL 369

Query: 370 FPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFC 429
            PLY +LNQRLP FDNTMD+IHT+G MDGW+D+LL+DF+L+DWDRVLRPGGLLWIDRFFC
Sbjct: 370 IPLYISLNQRLPFFDNTMDMIHTTGLMDGWIDLLLMDFVLYDWDRVLRPGGLLWIDRFFC 429

Query: 430 DRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEVYLSALLEKPPRAI 461
            +KDLDDYMYMFLQFRYKKHKW+I+PKSK+EVYLSALLEKPPRAI
Sbjct: 430 KKKDLDDYMYMFLQFRYKKHKWAISPKSKDEVYLSALLEKPPRAI 463

BLAST of IVF0012623 vs. TAIR 10
Match: AT4G01240.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 402.1 bits (1032), Expect = 5.9e-112
Identity = 207/459 (45.10%), Postives = 285/459 (62.09%), Query Frame = 0

Query: 25  LVIIMTTLSLFKFYSAGFIFHKDDVCRYFYTTTRHVYEETFDAKALSNRVEEVLDQLESL 84
           L  +++  +L KF     +   D  C Y  +     Y        +   +E  + ++   
Sbjct: 207 LAFLISVATLAKFNYITPLRFSDRFCNYHGSFDGDNYN---IGSQMRKTIETAIFKIHQE 266

Query: 85  QEKLESAVEEMEKNKQVLNNGNIRMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVDKNGQ 144
            + L++           +++ +  M  H  +L D V+  + + H     + LP  ++   
Sbjct: 267 MDDLKALEANSSTPPSSVSSASGSMFRHVAFLAD-VLSLVQSVH-----MELPSFEERSV 326

Query: 145 NSSTMKEE--PLINNFVVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYKKELED 204
               +K++      +F+ EEI+KYI  K +R+GK N       + +IGHAC   KK+LE+
Sbjct: 327 ADHPLKQQKGDPGEHFMREEIKKYIKIKPNRLGKQNFMGANGTFTSIGHACFAMKKDLEE 386

Query: 205 YMDYDIGSYCKDDWNLAQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKTPDDRNV 264
           YMDYD+G  C DDW LAQKLM++GCDPLPRRRC +R  ++Y KP+P+NESLWK PD+RNV
Sbjct: 387 YMDYDVGEICNDDWRLAQKLMVHGCDPLPRRRCFSRGPQLYHKPFPINESLWKLPDNRNV 446

Query: 265 RWSNYQCRNFSCFG--------------CFEI-EKEKLKW-----VISNSSLAVDFAISD 324
           RW  Y+C+NF+C                CF +   E  +W     +   ++   DF+I++
Sbjct: 447 RWGQYKCKNFACLASNTTARKGFFKCTDCFNLTHHESPRWLNRGEIDPETNQTADFSIAE 506

Query: 325 VLAIKPGEIRIGLDFGISTGSFAARMREENVTIVTTALNLGAPFNEFIALRGLFPLYSTL 384
           VL IKPGEIRIGLDF I TG+FAARMRE+NVTIV+  +NLGAPFNE IALRGL PLY T+
Sbjct: 507 VLEIKPGEIRIGLDFSIGTGTFAARMREQNVTIVSATINLGAPFNEMIALRGLVPLYLTV 566

Query: 385 NQRLPLFDNTMDLIHTSGFMDGWLDMLLLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDD 444
           NQRLP FD+T+D+IHT+ F+DGW+D++LLDF+LFDWDRVLRPGGLLWID FFC ++D+ D
Sbjct: 567 NQRLPFFDSTLDMIHTTRFLDGWIDLILLDFVLFDWDRVLRPGGLLWIDGFFCLKEDVSD 626

Query: 445 YMYMFLQFRYKKHKWSIAP---KSKNEVYLSALLEKPPR 459
           YM  F   RY+KHKW + P   K   EV+ SA+LEKPPR
Sbjct: 627 YMEAFKALRYRKHKWVVVPKKDKDDKEVFFSAVLEKPPR 656

BLAST of IVF0012623 vs. TAIR 10
Match: AT3G27230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 245.7 bits (626), Expect = 7.0e-65
Identity = 129/312 (41.35%), Postives = 186/312 (59.62%), Query Frame = 0

Query: 159 VVEEIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNL 218
           +V E++ ++   +  +GK +      + +++GH+C      L  YM Y +   C DDW+L
Sbjct: 102 IVPELKLFLQRHQLPLGKDSRTGITEMVSSVGHSCGKSTDLLSQYMSYKVFDRCPDDWSL 161

Query: 219 AQKLMLNGCDPLPRRRCLTRASKVYQKPYPLNESLWKTPDDRNVRWSNYQCRNFSCF--- 278
            QKL+L  C+PLPRRRCL +  +  Q      +SLW++  +++V WS   C++F C    
Sbjct: 162 GQKLILRACEPLPRRRCLAKTVQ-KQDLSKSPDSLWRSVSNKSVNWSGLGCKSFDCLKGK 221

Query: 279 -------GCFEIEKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFAARM 338
                  GCF++  EK ++V        DF I DVL +  G+IRIG D    +G+FAARM
Sbjct: 222 KLSKECVGCFDLGVEKDRFVKVKGK--NDFLIDDVLGLGSGKIRIGFDISGGSGTFAARM 281

Query: 339 REENVTIVTTALNLGAPFNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGWLDM 398
            E+NVT++T  LN GAPF+EFIA RGLFPL+ +L+ R P  DN  DLIH S  +D     
Sbjct: 282 AEKNVTVITNTLNNGAPFSEFIAARGLFPLFLSLDHRFPFLDNVFDLIHASSGLDVEGKA 341

Query: 399 LLLDFILFDWDRVLRPGGLLWIDRFFC-DRKDLDDYMYMFLQFRYKKHKWSIAPKSKNEV 458
             L+F++FD DRVL+P GL W+D F+C + +   +   M  +F YKK KW I  K+  +V
Sbjct: 342 EKLEFLMFDLDRVLKPRGLFWLDNFYCANDEKKKELTRMIERFGYKKLKWVIGEKADAQV 401

Query: 459 YLSALLEKPPRA 460
           YLSA+L+KP RA
Sbjct: 402 YLSAVLQKPVRA 410

BLAST of IVF0012623 vs. TAIR 10
Match: AT5G40830.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-64
Identity = 138/376 (36.70%), Postives = 216/376 (57.45%), Query Frame = 0

Query: 102 LNNGNIRMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVE 161
           L++ N+ ++     L   +++ + ++H  L Q+    +   G  S  + ++      V +
Sbjct: 56  LHSNNVSLVSQHLSL---ILREIDSSHHTLTQMEKQII---GYESLDLSQQE-----VPQ 115

Query: 162 EIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQK 221
           E++ ++   +  +GK +      +  ++GH+C +    L  YM Y++   C DDW+LAQK
Sbjct: 116 ELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQK 175

Query: 222 LMLNGCDPLPRRRCLTRASKVYQKP----YPLNESLWKTPDDRNVRWSNYQCRNFSCF-- 281
           L+L  C+PLPRRRCL   +K   KP    +P  +SLW+   + +V WS   C++F C   
Sbjct: 176 LILRACEPLPRRRCL---AKTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKG 235

Query: 282 --------GCFEI--EKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFA 341
                   GCF++    EK ++V  N     DF I DVL +  G+IRIG D    +G+FA
Sbjct: 236 KKLSRDCVGCFDLATSHEKDRFVKVNGK--TDFLIDDVLDLGDGKIRIGFDISSGSGTFA 295

Query: 342 ARMREENVTIVTTALNLGAPFNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGW 401
           ARM E+NV I++  LN+ APF+EFIA RG+FPL+ +L+QRLP +DN  DLIH S  +D  
Sbjct: 296 ARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLA 355

Query: 402 LDML--LLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHKWSIAPK 459
           +      L+F++FD DR+L+PGGL W+D F+C   +    +   ++ F YKK KW +  K
Sbjct: 356 VSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEK 413

BLAST of IVF0012623 vs. TAIR 10
Match: AT5G40830.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 1.2e-64
Identity = 138/376 (36.70%), Postives = 216/376 (57.45%), Query Frame = 0

Query: 102 LNNGNIRMIEHKKYLEDEVIKPLYNAHIALRQIRLPKVDKNGQNSSTMKEEPLINNFVVE 161
           L++ N+ ++     L   +++ + ++H  L Q+    +   G  S  + ++      V +
Sbjct: 56  LHSNNVSLVSQHLSL---ILREIDSSHHTLTQMEKQII---GYESLDLSQQE-----VPQ 115

Query: 162 EIRKYISPKKSRIGKLNMYMTENIYNTIGHACVLYKKELEDYMDYDIGSYCKDDWNLAQK 221
           E++ ++   +  +GK +      +  ++GH+C +    L  YM Y++   C DDW+LAQK
Sbjct: 116 ELKLFLQQHQLPLGKDSRTGITQMVASVGHSCEMSLDLLSQYMSYNVFEKCPDDWSLAQK 175

Query: 222 LMLNGCDPLPRRRCLTRASKVYQKP----YPLNESLWKTPDDRNVRWSNYQCRNFSCF-- 281
           L+L  C+PLPRRRCL   +K   KP    +P  +SLW+   + +V WS   C++F C   
Sbjct: 176 LILRACEPLPRRRCL---AKTVHKPGLALFP--DSLWRPVGNSSVNWSGLGCKSFECLKG 235

Query: 282 --------GCFEI--EKEKLKWVISNSSLAVDFAISDVLAIKPGEIRIGLDFGISTGSFA 341
                   GCF++    EK ++V  N     DF I DVL +  G+IRIG D    +G+FA
Sbjct: 236 KKLSRDCVGCFDLATSHEKDRFVKVNGK--TDFLIDDVLDLGDGKIRIGFDISSGSGTFA 295

Query: 342 ARMREENVTIVTTALNLGAPFNEFIALRGLFPLYSTLNQRLPLFDNTMDLIHTSGFMDGW 401
           ARM E+NV I++  LN+ APF+EFIA RG+FPL+ +L+QRLP +DN  DLIH S  +D  
Sbjct: 296 ARMAEKNVNIISNTLNIDAPFSEFIAARGIFPLFMSLDQRLPFYDNVFDLIHASNGLDLA 355

Query: 402 LDML--LLDFILFDWDRVLRPGGLLWIDRFFCDRKDLDDYMYMFLQ-FRYKKHKWSIAPK 459
           +      L+F++FD DR+L+PGGL W+D F+C   +    +   ++ F YKK KW +  K
Sbjct: 356 VSNKPEKLEFLMFDLDRILKPGGLFWLDNFYCGNDEKKRVLTRLIERFGYKKLKWVVGEK 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWB79.3e-5435.51Probable methyltransferase At1g29790 OS=Arabidopsis thaliana OX=3702 GN=CLSC5 PE... [more]
Match NameE-valueIdentityDescription
A0A5D3CEF42.3e-26597.04Putative Methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3BQC02.3e-26597.04uncharacterized protein LOC103492170 OS=Cucumis melo OX=3656 GN=LOC103492170 PE=... [more]
A0A6J1CY711.5e-23284.99uncharacterized protein LOC111015349 OS=Momordica charantia OX=3673 GN=LOC111015... [more]
A0A6J1I1D32.9e-23184.42uncharacterized protein LOC111468555 OS=Cucurbita maxima OX=3661 GN=LOC111468555... [more]
A0A6J1EZZ02.0e-22983.89uncharacterized protein LOC111440913 OS=Cucurbita moschata OX=3662 GN=LOC1114409... [more]
Match NameE-valueIdentityDescription
XP_008450643.10.097.04PREDICTED: uncharacterized protein LOC103492170 [Cucumis melo] >KAA0050891.1 put... [more]
XP_011659893.10.094.08uncharacterized protein LOC101207467 [Cucumis sativus] >KAE8653367.1 hypothetica... [more]
XP_038880508.18.26e-31391.45uncharacterized protein LOC120072172 [Benincasa hispida][more]
XP_022146046.18.78e-29584.99uncharacterized protein LOC111015349 [Momordica charantia][more]
XP_022969573.14.48e-29384.42uncharacterized protein LOC111468555 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G05390.11.4e-18568.82FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
AT4G01240.15.9e-11245.10S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G27230.17.0e-6541.35S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.11.2e-6436.70S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G40830.21.2e-6436.70S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 67..105
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 281..454
e-value: 2.7E-6
score: 28.7
NoneNo IPR availablePANTHERPTHR44067:SF9F22F7.17 PROTEINcoord: 8..459
NoneNo IPR availablePANTHERPTHR44067S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASE SUPERFAMILY PROTEIN-RELATEDcoord: 8..459
IPR004159Putative S-adenosyl-L-methionine-dependent methyltransferasePFAMPF03141Methyltransf_29coord: 303..411
e-value: 1.6E-6
score: 27.0
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 295..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0012623.1IVF0012623.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0043231 intracellular membrane-bounded organelle
molecular_function GO:0008168 methyltransferase activity