IVF0012234 (gene) Melon (IVF77) v1

BLAST of IVF0012234 vs. ExPASy Swiss-Prot
Match: Q9M0Z3 (K(+) efflux antiporter 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA3 PE=1 SV=2)

HSP 1 Score: 889.4 bits (2297), Expect = 3.0e-257
Identity = 505/776 (65.08%), Postives = 587/776 (75.64%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           M+  +ML  +SCC S +   Y  VKQ        H+V          H   + K +VP  
Sbjct: 1   MAISTMLGSISCCPSPKG--YEMVKQ--------HSVRL-------KHCVFTVKSSVP-- 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
            ++   ND  +    F N      F    +  Y           R+ T A +DVA+AVDV
Sbjct: 61  VYSEGVND-GIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG+IRN+ DVKVLSEWGI
Sbjct: 121 INDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP NGA+GTKILEFLFH+
Sbjct: 181 LFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHS 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRF           DIAVVPL
Sbjct: 241 RPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LRR+FEVVAE RSSEAFV
Sbjct: 301 LVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QESVR+GFLLSQGGEF F
Sbjct: 421 GTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F+ E+    +K  + VN
Sbjct: 481 VVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FD +E +VI+GFGQMGQ  +            FL   L S  D +  GWPY+ FD++ +V
Sbjct: 541 FDVSESIVIIGFGQMGQVLA-----------NFLSTPLVS--DSDLVGWPYIGFDLNPAV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VK SRKLGFP+LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP  PIY
Sbjct: 601 VKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARA+D+ HLL+LK AGATDAILE+AETSLQLGSKLL G GVMSD VSFLS++ R+SMEIQ
Sbjct: 661 ARAQDLPHLLELKKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQ 720

Query: 721 AQDALDKS----------------------NEQELEIMKPLQIRVKDSIESPESEL 744
           AQ+ +  S                       ++++++MKP+Q++  DS     +E+
Sbjct: 721 AQEEITASETNAGLKPMQMKASDINVVSAATQKQVQLMKPMQMKASDSNSDSAAEI 743

BLAST of IVF0012234 vs. ExPASy Swiss-Prot
Match: O65272 (K(+) efflux antiporter 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA2 PE=1 SV=2)

HSP 1 Score: 307.0 bits (785), Expect = 6.4e-82
Identity = 208/582 (35.74%), Postives = 314/582 (53.95%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R            D+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEA 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E 
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 809

Query: 366  FVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFV 425
            F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+
Sbjct: 810  FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 869

Query: 426  TTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEF 485
            T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR+G LL+ GGEF
Sbjct: 870  TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 929

Query: 486  GFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA-- 545
             FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ +++ +D  +  
Sbjct: 930  AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIASRFELQDVRSLL 989

Query: 546  --DTVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAF 605
              ++   D    ++I GFG++GQ  +           + L   L           P+VA 
Sbjct: 990  PVESETDDLQGHIIICGFGRIGQIIA-----------QLLSERLI----------PFVAL 1049

Query: 606  DIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAF 665
            D+    V   R L  PV +GD     VL   G     A  +      A    V  L   F
Sbjct: 1050 DVSSDRVAIGRSLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYF 1106

Query: 666  PAIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            P +  + RA DV H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 PNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1106

BLAST of IVF0012234 vs. ExPASy Swiss-Prot
Match: Q9ZTZ7 (K(+) efflux antiporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KEA1 PE=1 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 3.2e-81
Identity = 205/581 (35.28%), Postives = 314/581 (54.04%), Query Frame = 0

Query: 127  DTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILFL 186
            D L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   + ++E+G++FL
Sbjct: 591  DFLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFL 650

Query: 187  LFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSD 246
            LF +GLELS  RL ++ ++ FG+G  QV+++            G  G             
Sbjct: 651  LFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVVGLLAHYVAGQAG------------- 710

Query: 247  LVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVI 306
                     AIVIG  L+LSS+A VLQ+L E+GE  +R            D+AVV LL++
Sbjct: 711  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVLLFQDLAVVVLLIL 770

Query: 307  LPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAF 366
            +P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E F
Sbjct: 771  IPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPIYKQIAENRNAEIF 830

Query: 367  VALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVT 426
             A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 831  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 890

Query: 427  TGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFG 486
             G SID +LL   +P ++  L  LI  KT+++  +G   G++   ++R+G LL+ GGEF 
Sbjct: 891  VGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAIRVGLLLAPGGEFA 950

Query: 487  FVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA--- 546
            FV F  A   G++  +L+ LL ++V +SMA+TP L   G    + I+ +++  D  +   
Sbjct: 951  FVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGG----QLIASRFELHDVRSLLP 1010

Query: 547  -DTVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFD 606
             ++   D    ++I GFG++GQ  +           + L   L           P+VA D
Sbjct: 1011 VESETDDLQGHIIICGFGRVGQIIA-----------QLLSERLI----------PFVALD 1070

Query: 607  IDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFP 666
            +    V   R L  PV +GD     VL   G     A +V      A    V  L   +P
Sbjct: 1071 VSSDRVTIGRSLDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYP 1126

Query: 667  AIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
             +  + RA DVVH L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1131 NVKTFVRAHDVVHGLNLEKAGATAVVPETLEPSLQLAAAVL 1126

BLAST of IVF0012234 vs. ExPASy Swiss-Prot
Match: Q1C2S9 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis bv. Antiqua (strain Antiqua) OX=360102 GN=kefB PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 6.6e-63
Identity = 189/589 (32.09%), Postives = 298/589 (50.59%), Query Frame = 0

Query: 129 LTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGV--IRNIVDVKVLSEWGILFLLFE 188
           L FL   VV VP+ +++    +LG+  AGI +  +G+  IR++ ++   SE G++FL+F 
Sbjct: 11  LVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFI 70

Query: 189 MGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVN 248
           +GLEL+ A+L  L R  FG+G  QV+++     A    T         +F + A      
Sbjct: 71  IGLELNPAKLWQLRRSIFGVGAGQVVITAAVLGALLYFT---------QFAWQA------ 130

Query: 249 IRSVDEAIVIGAALSLSSSAFVLQLLAEKG-----------ELATRFDIAVVPLLVILPV 308
                 A++ G  L++SS+A  LQL+ EKG            +    D+AV+P L ++P+
Sbjct: 131 ------AVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPI 190

Query: 309 LESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLT 368
           L + N G  + W  +    LK     G+L +GG+ +LR +F  +  +   E F A  LL 
Sbjct: 191 L-AGNEGGANDWVKI---GLKIAAFAGML-IGGRYLLRPLFRYIVASGVREVFTAAALLV 250

Query: 369 VAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDM 428
           V G++L    LG S  LG F+AG +LAE+ F+ ++E  I PF+GLLLGLFF++ G ++D+
Sbjct: 251 VLGSALFMDALGLSMALGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDL 310

Query: 429 QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLA 488
            +LF    +VL  +  L+ IK+ I+  +    GL     ++   +LSQGGEF FV+FS A
Sbjct: 311 GVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAA 370

Query: 489 NRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEP 548
               VL  E   LL+++V LSM  TPLL +        +  +Y  ++++ +    +  +P
Sbjct: 371 FSQRVLNAEQLALLLVVVTLSMMTTPLLMQV---IDRILVRRYNAQEESDEKPFVEDNDP 430

Query: 549 -VVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSR 608
            V+IVGFG+ GQ             G+ L+                   + D+S V   R
Sbjct: 431 QVIIVGFGRFGQVI-----------GRLLMANKMR----------ITVLERDVSAVSMMR 490

Query: 609 KLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKD 668
           K G+ V YGD +   +L++AG    KA+++   E + T+  V   +  FP + I ARA+ 
Sbjct: 491 KYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMALVHLCQQHFPNLHILARARG 549

Query: 669 VVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVR 704
            V   +L   G  D   E   ++L+LG K L  LG+   Q     Q  R
Sbjct: 551 RVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFR 549

BLAST of IVF0012234 vs. ExPASy Swiss-Prot
Match: Q8ZJC4 (Glutathione-regulated potassium-efflux system protein KefB OS=Yersinia pestis OX=632 GN=kefB PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 6.6e-63
Identity = 189/589 (32.09%), Postives = 298/589 (50.59%), Query Frame = 0

Query: 129 LTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGV--IRNIVDVKVLSEWGILFLLFE 188
           L FL   VV VP+ +++    +LG+  AGI +  +G+  IR++ ++   SE G++FL+F 
Sbjct: 11  LVFLFAAVVAVPIAQRLGIGAVLGYLIAGIAIGPWGLGFIRDVDEILHFSELGVVFLMFI 70

Query: 189 MGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVN 248
           +GLEL+ A+L  L R  FG+G  QV+++     A    T         +F + A      
Sbjct: 71  IGLELNPAKLWQLRRSIFGVGAGQVVITAAVLGALLYFT---------QFAWQA------ 130

Query: 249 IRSVDEAIVIGAALSLSSSAFVLQLLAEKG-----------ELATRFDIAVVPLLVILPV 308
                 A++ G  L++SS+A  LQL+ EKG            +    D+AV+P L ++P+
Sbjct: 131 ------AVIGGVGLAMSSTAMALQLMREKGMNRNEGGQLGFSVLLFQDMAVIPALALIPI 190

Query: 309 LESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLT 368
           L + N G  + W  +    LK     G+L +GG+ +LR +F  +  +   E F A  LL 
Sbjct: 191 L-AGNEGGANDWVKI---GLKIAAFAGML-IGGRYLLRPLFRYIVASGVREVFTAAALLV 250

Query: 369 VAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDM 428
           V G++L    LG S  LG F+AG +LAE+ F+ ++E  I PF+GLLLGLFF++ G ++D+
Sbjct: 251 VLGSALFMDALGLSMALGTFIAGILLAESEFQHELEIAIEPFKGLLLGLFFISVGMALDL 310

Query: 429 QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLA 488
            +LF    +VL  +  L+ IK+ I+  +    GL     ++   +LSQGGEF FV+FS A
Sbjct: 311 GVLFTHLLDVLLGVLALVFIKSAILYGLARVFGLRRSVRLQFAGVLSQGGEFAFVLFSAA 370

Query: 489 NRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEP 548
               VL  E   LL+++V LSM  TPLL +        +  +Y  ++++ +    +  +P
Sbjct: 371 FSQRVLNAEQLALLLVVVTLSMMTTPLLMQV---IDRILVRRYNAQEESDEKPFVEDNDP 430

Query: 549 -VVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSR 608
            V+IVGFG+ GQ             G+ L+                   + D+S V   R
Sbjct: 431 QVIIVGFGRFGQVI-----------GRLLMANKMR----------ITVLERDVSAVSMMR 490

Query: 609 KLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKD 668
           K G+ V YGD +   +L++AG    KA+++   E + T+  V   +  FP + I ARA+ 
Sbjct: 491 KYGYKVYYGDATELELLRAAGAEKAKAIVITCNEPEDTMALVHLCQQHFPNLHILARARG 549

Query: 669 VVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVR 704
            V   +L   G  D   E   ++L+LG K L  LG+   Q     Q  R
Sbjct: 551 RVEAHELLQNGVKDFTRETFSSALELGRKTLLELGMHPHQAYRAQQHFR 549

BLAST of IVF0012234 vs. ExPASy TrEMBL
Match: A0A1S3C1C7 (K(+) efflux antiporter 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495357 PE=4 SV=1)

HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 793/822 (96.47%), Postives = 794/822 (96.59%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV
Sbjct: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FDATEPVVIVGFGQMGQ  +            FL   LASGIDGNTPGWPYVAFDIDLSV
Sbjct: 541 FDATEPVVIVGFGQMGQVLA-----------NFLSTPLASGIDGNTPGWPYVAFDIDLSV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY
Sbjct: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ
Sbjct: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720

Query: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF
Sbjct: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK 812
           QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK
Sbjct: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK 811

BLAST of IVF0012234 vs. ExPASy TrEMBL
Match: A0A0A0K7K4 (RCK N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G067510 PE=4 SV=1)

HSP 1 Score: 1441.4 bits (3730), Expect = 0.0e+00
Identity = 772/823 (93.80%), Postives = 786/823 (95.50%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFG+MLEPVSCCQSSQSQIY AVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGTMLEPVSCCQSSQSQIYGAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N+WRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRER+RT AALDVAAAVDV
Sbjct: 61  TSNYWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERIRTHAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FDATEPVVIVGFGQMGQ  +            FL   LASG+DGNTPGWPYVAFDIDLSV
Sbjct: 541 FDATEPVVIVGFGQMGQVLA-----------NFLSTPLASGLDGNTPGWPYVAFDIDLSV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY
Sbjct: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ
Sbjct: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720

Query: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           AQDA+DKSNEQELEIMKPLQIRVKDSIESPE+ELSRLN +DKTQILNGKEVDQMKQGTVF
Sbjct: 721 AQDAIDKSNEQELEIMKPLQIRVKDSIESPENELSRLNLKDKTQILNGKEVDQMKQGTVF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPP-THITATDGLEK 812
           +K EDLDGNGVLYC+LDTENN  E+NVVEPP THI ATDG+EK
Sbjct: 781 EKAEDLDGNGVLYCDLDTENNFLEENVVEPPTTHIAATDGVEK 812

BLAST of IVF0012234 vs. ExPASy TrEMBL
Match: E5GCQ5 (Glutathione-regulated potassium-efflux system protein kefb OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 1426.0 bits (3690), Expect = 0.0e+00
Identity = 769/804 (95.65%), Postives = 770/804 (95.77%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRF           DIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV      LGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV------LGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQF 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQ 
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 541

Query: 559 FSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 618
            +            FL   LASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR
Sbjct: 542 LA-----------NFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 601

Query: 619 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 678
           PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT
Sbjct: 602 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 661

Query: 679 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 738
           DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP
Sbjct: 662 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 721

Query: 739 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 798
           LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT
Sbjct: 722 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 781

Query: 799 ENNLFEQNVVEPPTHITATDGLEK 812
           ENNLFEQNVVEPPTHITATDGLEK
Sbjct: 782 ENNLFEQNVVEPPTHITATDGLEK 788

BLAST of IVF0012234 vs. ExPASy TrEMBL
Match: A0A5A7SQK8 (K(+) efflux antiporter 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001700 PE=4 SV=1)

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 766/804 (95.27%), Postives = 767/804 (95.40%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRF           DIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQF 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV+         
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVL--------- 541

Query: 559 FSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 618
                         FL   LASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR
Sbjct: 542 ------------ANFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 601

Query: 619 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 678
           PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT
Sbjct: 602 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 661

Query: 679 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 738
           DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP
Sbjct: 662 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 721

Query: 739 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 798
           LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT
Sbjct: 722 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 781

Query: 799 ENNLFEQNVVEPPTHITATDGLEK 812
           ENNLFEQNVVEPPTHITATDGLEK
Sbjct: 782 ENNLFEQNVVEPPTHITATDGLEK 784

BLAST of IVF0012234 vs. ExPASy TrEMBL
Match: A0A6J1E2E0 (K(+) efflux antiporter 3, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111025388 PE=4 SV=1)

HSP 1 Score: 1318.1 bits (3410), Expect = 0.0e+00
Identity = 716/830 (86.27%), Postives = 752/830 (90.60%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MS G+ML+PVSCCQSSQSQIYS VKQKNP RAYSH +HQLCGPSFNLH   SKKVAVPS 
Sbjct: 1   MSLGTMLDPVSCCQSSQSQIYSVVKQKNPMRAYSHIIHQLCGPSFNLHNLQSKKVAVPSF 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTF---KVVGQNGYNWSNRRPKQRERLRTRAALDVAAA 120
           T N  RN YSLVPVLFHN +TTL F   KVVG++G+NWSN RPKQRERLRT AA+DVAAA
Sbjct: 61  TINCLRNGYSLVPVLFHNSSTTLAFSSSKVVGRDGFNWSNHRPKQRERLRTCAAVDVAAA 120

Query: 121 VDVINDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSE 180
           VDVINDLGLDTLTFLAVTV+VVPLFR+VKASPILGFFFAGIVLNQFGVIRN++DVKVLSE
Sbjct: 121 VDVINDLGLDTLTFLAVTVIVVPLFRRVKASPILGFFFAGIVLNQFGVIRNLIDVKVLSE 180

Query: 181 WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFL 240
           WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELP NGA+GT+ILEFL
Sbjct: 181 WGILFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPPNGAIGTRILEFL 240

Query: 241 FHARSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAV 300
           FHARSDLVNIRS+DEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAV
Sbjct: 241 FHARSDLVNIRSIDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLFQDIAV 300

Query: 301 VPLLVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE 360
           VPLLVILPVLESQNLGT+SIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE
Sbjct: 301 VPLLVILPVLESQNLGTQSIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSE 360

Query: 361 AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF 420
           AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF
Sbjct: 361 AFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFF 420

Query: 421 VTTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGE 480
           VTTGTSID QLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIG LLSQGGE
Sbjct: 421 VTTGTSIDTQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGLLLSQGGE 480

Query: 481 FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAAD 540
           FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKY+TEDKAA+
Sbjct: 481 FGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYETEDKAAE 540

Query: 541 TVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDID 600
           TVNFDA+EPVVIVGFGQMGQ  +            FL   L SG DG+TPGWPYVAFDID
Sbjct: 541 TVNFDASEPVVIVGFGQMGQVLA-----------NFLSAPLVSGGDGDTPGWPYVAFDID 600

Query: 601 LSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAI 660
           LSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVM+TEKK TI+AVQ+LRLAFPAI
Sbjct: 601 LSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMYTEKKTTIEAVQRLRLAFPAI 660

Query: 661 PIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSM 720
           PIY RAKDVVHLLDLK AGATDAILEDAETSLQLGSKLLKGLGVMSDQVSF+SQ+VR+SM
Sbjct: 661 PIYGRAKDVVHLLDLKNAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFISQIVRDSM 720

Query: 721 EIQAQDALDKSNEQELEIMKPLQIRVKDSIE--------SPESELSRLNREDKTQILNGK 780
           EIQAQDALDKS+EQELEIMKP+QIRVKDS+E        SPE  LSRLN+ED + ILNGK
Sbjct: 721 EIQAQDALDKSDEQELEIMKPMQIRVKDSLESGLPSLSTSPEDNLSRLNQEDNSLILNGK 780

Query: 781 EVDQMKQGTVFQKPEDLDGNGVLYCELDTENNLFEQNVVEPPT-HITATD 808
           E++Q KQ  VFQK EDLDG GVLYCELD+ENN  +QNV EPPT  I ATD
Sbjct: 781 EMEQRKQDAVFQKAEDLDGKGVLYCELDSENNFLDQNVAEPPTPFIAATD 819

BLAST of IVF0012234 vs. NCBI nr
Match: XP_008455102.1 (PREDICTED: K(+) efflux antiporter 3, chloroplastic [Cucumis melo])

HSP 1 Score: 1479 bits (3830), Expect = 0.0
Identity = 793/822 (96.47%), Postives = 794/822 (96.59%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV
Sbjct: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FDATEPVVIVGFGQMGQ  +            FL   LASGIDGNTPGWPYVAFDIDLSV
Sbjct: 541 FDATEPVVIVGFGQMGQVLA-----------NFLSTPLASGIDGNTPGWPYVAFDIDLSV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY
Sbjct: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ
Sbjct: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720

Query: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF
Sbjct: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK 811
           QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK
Sbjct: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK 811

BLAST of IVF0012234 vs. NCBI nr
Match: XP_011658846.1 (K(+) efflux antiporter 3, chloroplastic [Cucumis sativus] >KGN43796.1 hypothetical protein Csa_017320 [Cucumis sativus])

HSP 1 Score: 1442 bits (3733), Expect = 0.0
Identity = 772/823 (93.80%), Postives = 786/823 (95.50%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MSFG+MLEPVSCCQSSQSQIY AVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC
Sbjct: 1   MSFGTMLEPVSCCQSSQSQIYGAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N+WRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRER+RT AALDVAAAVDV
Sbjct: 61  TSNYWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERIRTHAALDVAAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FDATEPVVIVGFGQMGQ  +            FL   LASG+DGNTPGWPYVAFDIDLSV
Sbjct: 541 FDATEPVVIVGFGQMGQVLA-----------NFLSTPLASGLDGNTPGWPYVAFDIDLSV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY
Sbjct: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ
Sbjct: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720

Query: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           AQDA+DKSNEQELEIMKPLQIRVKDSIESPE+ELSRLN +DKTQILNGKEVDQMKQGTVF
Sbjct: 721 AQDAIDKSNEQELEIMKPLQIRVKDSIESPENELSRLNLKDKTQILNGKEVDQMKQGTVF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPT-HITATDGLEK 811
           +K EDLDGNGVLYC+LDTENN  E+NVVEPPT HI ATDG+EK
Sbjct: 781 EKAEDLDGNGVLYCDLDTENNFLEENVVEPPTTHIAATDGVEK 812

BLAST of IVF0012234 vs. NCBI nr
Match: ADN34254.1 (glutathione-regulated potassium-efflux system protein kefb [Cucumis melo subsp. melo])

HSP 1 Score: 1426 bits (3691), Expect = 0.0
Identity = 769/804 (95.65%), Postives = 770/804 (95.77%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRF           DIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV      LGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVV------LGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQF 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQ 
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQV 541

Query: 559 FSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 618
            +            FL   LASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR
Sbjct: 542 LA-----------NFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 601

Query: 619 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 678
           PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT
Sbjct: 602 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 661

Query: 679 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 738
           DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP
Sbjct: 662 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 721

Query: 739 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 798
           LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT
Sbjct: 722 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 781

Query: 799 ENNLFEQNVVEPPTHITATDGLEK 811
           ENNLFEQNVVEPPTHITATDGLEK
Sbjct: 782 ENNLFEQNVVEPPTHITATDGLEK 788

BLAST of IVF0012234 vs. NCBI nr
Match: KAA0031415.1 (K(+) efflux antiporter 3 [Cucumis melo var. makuwa] >TYK06866.1 K(+) efflux antiporter 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 766/804 (95.27%), Postives = 767/804 (95.40%), Query Frame = 0

Query: 19  QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 78
           QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN
Sbjct: 2   QIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSCTFNHWRNDYSLVPVLFHN 61

Query: 79  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 138
           GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV
Sbjct: 62  GATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDVINDLGLDTLTFLAVTVVV 121

Query: 139 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 198
           VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA
Sbjct: 122 VPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGILFLLFEMGLELSFARLKA 181

Query: 199 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 258
           LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA
Sbjct: 182 LARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSDLVNIRSVDEAIVIGA 241

Query: 259 ALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVILPVLESQNLGTESIW 318
           ALSLSSSAFVLQLLAEKGELATRF           DIAVVPLLVILPVLESQNLGTESIW
Sbjct: 242 ALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPLLVILPVLESQNLGTESIW 301

Query: 319 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 378
           PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG
Sbjct: 302 PMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFVALCLLTVAGTSLITQKLG 361

Query: 379 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 438
           FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA
Sbjct: 362 FSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTTGTSIDMQLLFREWPNVLA 421

Query: 439 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 498
           LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK
Sbjct: 422 LLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGFVVFSLANRLGVLPLELNK 481

Query: 499 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVIVGFGQMGQF 558
           LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVV+         
Sbjct: 482 LLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVNFDATEPVVLA-------- 541

Query: 559 FSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 618
                         FL   LASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR
Sbjct: 542 -------------NFLSTPLASGIDGNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSR 601

Query: 619 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 678
           PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT
Sbjct: 602 PAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGAT 661

Query: 679 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 738
           DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP
Sbjct: 662 DAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQAQDALDKSNEQELEIMKP 721

Query: 739 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 798
           LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT
Sbjct: 722 LQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVFQKPEDLDGNGVLYCELDT 781

Query: 799 ENNLFEQNVVEPPTHITATDGLEK 811
           ENNLFEQNVVEPPTHITATDGLEK
Sbjct: 782 ENNLFEQNVVEPPTHITATDGLEK 784

BLAST of IVF0012234 vs. NCBI nr
Match: XP_038887837.1 (K(+) efflux antiporter 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1414 bits (3660), Expect = 0.0
Identity = 760/822 (92.46%), Postives = 776/822 (94.40%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           MS G+MLE VSCCQSSQSQIYSAVKQKNP RAYSHTVHQLCGPSFNLHYSHSKK+AV SC
Sbjct: 1   MSLGNMLESVSCCQSSQSQIYSAVKQKNPLRAYSHTVHQLCGPSFNLHYSHSKKLAVSSC 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
           T N  RNDYSLVPVLF++GATTLTFKVV QNGY+WSNRRPKQR RL TRAALDV+AAVDV
Sbjct: 61  TLNCGRNDYSLVPVLFYSGATTLTFKVVRQNGYSWSNRRPKQRIRLGTRAALDVSAAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLGLDTLTFLAVTVVVVPLFR++KASPILGFFFAGIVLNQFGVIRNI+DVKVLSEWGI
Sbjct: 121 INDLGLDTLTFLAVTVVVVPLFRRIKASPILGFFFAGIVLNQFGVIRNIIDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGA+GT+ILE+LFHA
Sbjct: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAIGTRILEYLFHA 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF           DIAVVPL
Sbjct: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV
Sbjct: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF
Sbjct: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN
Sbjct: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FDATEPVVIVGFGQMGQ  +            FL   LASG DGNTPGWPYVAFDIDLSV
Sbjct: 541 FDATEPVVIVGFGQMGQVLA-----------NFLSTPLASGTDGNTPGWPYVAFDIDLSV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY
Sbjct: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARAKDVVHLLDLK AGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ
Sbjct: 661 ARAKDVVHLLDLKNAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720

Query: 721 AQDALDKSNEQELEIMKPLQIRVKDSIESPESELSRLNREDKTQILNGKEVDQMKQGTVF 780
           AQDALDKSNEQELE+MKPLQIRVKDSIESPE+ELSRLNRED TQILNGKEVDQMK  T F
Sbjct: 721 AQDALDKSNEQELELMKPLQIRVKDSIESPENELSRLNREDNTQILNGKEVDQMKPDTFF 780

Query: 781 QKPEDLDGNGVLYCELDTENNLFEQNVVEPPTHITATDGLEK 811
           QKPEDLDGNGVLYCEL+TENN  EQNVVEPPTHITATDGLEK
Sbjct: 781 QKPEDLDGNGVLYCELETENNFLEQNVVEPPTHITATDGLEK 811

BLAST of IVF0012234 vs. TAIR 10
Match: AT4G04850.2 (K+ efflux antiporter 3 )

HSP 1 Score: 889.4 bits (2297), Expect = 2.1e-258
Identity = 505/776 (65.08%), Postives = 587/776 (75.64%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           M+  +ML  +SCC S +   Y  VKQ        H+V          H   + K +VP  
Sbjct: 1   MAISTMLGSISCCPSPKG--YEMVKQ--------HSVRL-------KHCVFTVKSSVP-- 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
            ++   ND  +    F N      F    +  Y           R+ T A +DVA+AVDV
Sbjct: 61  VYSEGVND-GIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG+IRN+ DVKVLSEWGI
Sbjct: 121 INDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP NGA+GTKILEFLFH+
Sbjct: 181 LFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHS 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRF           DIAVVPL
Sbjct: 241 RPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LRR+FEVVAE RSSEAFV
Sbjct: 301 LVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QESVR+GFLLSQGGEF F
Sbjct: 421 GTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F+ E+    +K  + VN
Sbjct: 481 VVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FD +E +VI+GFGQMGQ  +            FL   L S  D +  GWPY+ FD++ +V
Sbjct: 541 FDVSESIVIIGFGQMGQVLA-----------NFLSTPLVS--DSDLVGWPYIGFDLNPAV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAIPIY 660
           VK SRKLGFP+LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP  PIY
Sbjct: 601 VKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGSPIY 660

Query: 661 ARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLLKGLGVMSDQVSFLSQMVRNSMEIQ 720
           ARA+D+ HLL+LK AGATDAILE+AETSLQLGSKLL G GVMSD VSFLS++ R+SMEIQ
Sbjct: 661 ARAQDLPHLLELKKAGATDAILENAETSLQLGSKLLTGFGVMSDDVSFLSKVFRDSMEIQ 720

Query: 721 AQDALDKS----------------------NEQELEIMKPLQIRVKDSIESPESEL 744
           AQ+ +  S                       ++++++MKP+Q++  DS     +E+
Sbjct: 721 AQEEITASETNAGLKPMQMKASDINVVSAATQKQVQLMKPMQMKASDSNSDSAAEI 743

BLAST of IVF0012234 vs. TAIR 10
Match: AT4G04850.1 (K+ efflux antiporter 3 )

HSP 1 Score: 792.0 bits (2044), Expect = 4.6e-229
Identity = 445/657 (67.73%), Postives = 508/657 (77.32%), Query Frame = 0

Query: 1   MSFGSMLEPVSCCQSSQSQIYSAVKQKNPFRAYSHTVHQLCGPSFNLHYSHSKKVAVPSC 60
           M+  +ML  +SCC S +   Y  VKQ        H+V          H   + K +VP  
Sbjct: 1   MAISTMLGSISCCPSPKG--YEMVKQ--------HSVRL-------KHCVFTVKSSVP-- 60

Query: 61  TFNHWRNDYSLVPVLFHNGATTLTFKVVGQNGYNWSNRRPKQRERLRTRAALDVAAAVDV 120
            ++   ND  +    F N      F    +  Y           R+ T A +DVA+AVDV
Sbjct: 61  VYSEGVND-GIKLHSFGNLVKKKVFLDTSKRFYFQGRWSESSGRRVETYAGVDVASAVDV 120

Query: 121 INDLGLDTLTFLAVTVVVVPLFRKVKASPILGFFFAGIVLNQFGVIRNIVDVKVLSEWGI 180
           INDLG DTLTFL VTV++VP FR +KASPILGFFFAG+VLNQFG+IRN+ DVKVLSEWGI
Sbjct: 121 INDLGFDTLTFLMVTVIIVPAFRILKASPILGFFFAGVVLNQFGLIRNLTDVKVLSEWGI 180

Query: 181 LFLLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHA 240
           LFLLFEMGLELS ARLKALA+FAFGMGLTQV+L T AFTAFELP NGA+GTKILEFLFH+
Sbjct: 181 LFLLFEMGLELSLARLKALAKFAFGMGLTQVLLCTAAFTAFELPPNGAIGTKILEFLFHS 240

Query: 241 RSDLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPL 300
           R DLVNIRS+DEA+VIGAALSLSSSAFVLQLLAEKGEL TRF           DIAVVPL
Sbjct: 241 RPDLVNIRSIDEAVVIGAALSLSSSAFVLQLLAEKGELPTRFGSATLGILLLQDIAVVPL 300

Query: 301 LVILPVLESQNLGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAFV 360
           LVILPVLESQ++G ESIWPMLA+ES KALGGLG+LSLGGK  LRR+FEVVAE RSSEAFV
Sbjct: 301 LVILPVLESQDIGGESIWPMLAKESAKALGGLGILSLGGKFFLRRIFEVVAETRSSEAFV 360

Query: 361 ALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVTT 420
           ALCLLTVAGTSL+TQ LGFSDTLGAFLAGA+LAETNFRTQIEADIRPFRGLLLGLFFVTT
Sbjct: 361 ALCLLTVAGTSLVTQWLGFSDTLGAFLAGALLAETNFRTQIEADIRPFRGLLLGLFFVTT 420

Query: 421 GTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFGF 480
           GTSIDM++LFREWPNVL+LL GLI IKTLIITAIGPRVGLT QESVR+GFLLSQGGEF F
Sbjct: 421 GTSIDMEVLFREWPNVLSLLGGLIVIKTLIITAIGPRVGLTIQESVRVGFLLSQGGEFAF 480

Query: 481 VVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAADTVN 540
           VVFSLANRLGVLP ELNKLLII+VVLSMALTP LN+ GRKA++F+ E+    +K  + VN
Sbjct: 481 VVFSLANRLGVLPNELNKLLIIVVVLSMALTPYLNQLGRKAADFLDERLDPGEKIGEDVN 540

Query: 541 FDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFDIDLSV 600
           FD +E +VI+GFGQMGQ  +            FL   L S  D +  GWPY+ FD++ +V
Sbjct: 541 FDVSESIVIIGFGQMGQVLA-----------NFLSTPLVS--DSDLVGWPYIGFDLNPAV 600

Query: 601 VKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFPAI 647
           VK SRKLGFP+LYGDGSRP+VLQSAG+SSPKA+M+M+  KK T +AVQ+LRLAFP +
Sbjct: 601 VKESRKLGFPILYGDGSRPSVLQSAGVSSPKAIMIMYKGKKRTTEAVQRLRLAFPGV 624

BLAST of IVF0012234 vs. TAIR 10
Match: AT4G00630.1 (K+ efflux antiporter 2 )

HSP 1 Score: 307.0 bits (785), Expect = 4.5e-83
Identity = 208/582 (35.74%), Postives = 314/582 (53.95%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R            D+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEA 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E 
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLLLRPIYKQIAENRNAEI 809

Query: 366  FVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFV 425
            F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+
Sbjct: 810  FSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFM 869

Query: 426  TTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEF 485
            T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR+G LL+ GGEF
Sbjct: 870  TVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVRVGLLLAPGGEF 929

Query: 486  GFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA-- 545
             FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ +++ +D  +  
Sbjct: 930  AFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIASRFELQDVRSLL 989

Query: 546  --DTVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAF 605
              ++   D    ++I GFG++GQ  +           + L   L           P+VA 
Sbjct: 990  PVESETDDLQGHIIICGFGRIGQIIA-----------QLLSERLI----------PFVAL 1049

Query: 606  DIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAF 665
            D+    V   R L  PV +GD     VL   G     A  +      A    V  L   F
Sbjct: 1050 DVSSDRVAIGRSLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGANYRCVWALSKYF 1106

Query: 666  PAIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
            P +  + RA DV H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 PNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1106

BLAST of IVF0012234 vs. TAIR 10
Match: AT1G01790.1 (K+ efflux antiporter 1 )

HSP 1 Score: 304.7 bits (779), Expect = 2.3e-82
Identity = 205/581 (35.28%), Postives = 314/581 (54.04%), Query Frame = 0

Query: 127  DTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILFL 186
            D L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   + ++E+G++FL
Sbjct: 591  DFLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTRAIAEFGVVFL 650

Query: 187  LFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARSD 246
            LF +GLELS  RL ++ ++ FG+G  QV+++            G  G             
Sbjct: 651  LFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVVGLLAHYVAGQAG------------- 710

Query: 247  LVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLVI 306
                     AIVIG  L+LSS+A VLQ+L E+GE  +R            D+AVV LL++
Sbjct: 711  -------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRASFSVLLFQDLAVVVLLIL 770

Query: 307  LPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKLILRRVFEVVAEARSSEAF 366
            +P++   +    +G ++I   L   ++KA   +  +  GG+L+LR +++ +AE R++E F
Sbjct: 771  IPLISPNSSKGGIGFQAIAEALGLAAVKAAVAITAIIAGGRLLLRPIYKQIAENRNAEIF 830

Query: 367  VALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRPFRGLLLGLFFVT 426
             A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P+RGLLLGLFF+T
Sbjct: 831  SANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAPYRGLLLGLFFMT 890

Query: 427  TGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVRIGFLLSQGGEFG 486
             G SID +LL   +P ++  L  LI  KT+++  +G   G++   ++R+G LL+ GGEF 
Sbjct: 891  VGMSIDPKLLLSNFPVIVGTLGLLIVGKTMLVVIMGKLFGISIISAIRVGLLLAPGGEFA 950

Query: 487  FVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISEKYKTEDKAA--- 546
            FV F  A   G++  +L+ LL ++V +SMA+TP L   G    + I+ +++  D  +   
Sbjct: 951  FVAFGEAVNQGIMSPQLSSLLFLVVGISMAITPWLAAGG----QLIASRFELHDVRSLLP 1010

Query: 547  -DTVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGIDGNTPGWPYVAFD 606
             ++   D    ++I GFG++GQ  +           + L   L           P+VA D
Sbjct: 1011 VESETDDLQGHIIICGFGRVGQIIA-----------QLLSERLI----------PFVALD 1070

Query: 607  IDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKATIDAVQKLRLAFP 666
            +    V   R L  PV +GD     VL   G     A +V      A    V  L   +P
Sbjct: 1071 VSSDRVTIGRSLDLPVYFGDAGSKEVLHKIGAGRACAAVVALDAPGANYRCVWALSKFYP 1126

Query: 667  AIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
             +  + RA DVVH L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1131 NVKTFVRAHDVVHGLNLEKAGATAVVPETLEPSLQLAAAVL 1126

BLAST of IVF0012234 vs. TAIR 10
Match: AT4G00630.2 (K+ efflux antiporter 2 )

HSP 1 Score: 298.5 bits (763), Expect = 1.6e-80
Identity = 208/593 (35.08%), Postives = 314/593 (52.95%), Query Frame = 0

Query: 126  LDTLTFLAVTVVVVPLFRKVK-ASPILGFFFAGIVLNQFG--VIRNIVDVKVLSEWGILF 185
            LD L  L  +V+ VPLF+K+   SP+LG+  AGI++  +G  +IRN+   K ++E+G++F
Sbjct: 570  LDVLWLLLASVIFVPLFQKIPGGSPVLGYLAAGILIGPYGLSIIRNVHGTKAIAEFGVVF 629

Query: 186  LLFEMGLELSFARLKALARFAFGMGLTQVILSTIAFTAFELPTNGAVGTKILEFLFHARS 245
            LLF +GLELS  RL ++ ++ FG+G  QV+++            G  G            
Sbjct: 630  LLFNIGLELSVERLSSMKKYVFGLGSAQVLVTAAVIGLITHYVAGQAG------------ 689

Query: 246  DLVNIRSVDEAIVIGAALSLSSSAFVLQLLAEKGELATRF-----------DIAVVPLLV 305
                      AIVIG  L+LSS+A VLQ+L E+GE  +R            D+AVV LL+
Sbjct: 690  --------PAAIVIGNGLALSSTAVVLQVLQERGESTSRHGRATFSVLLFQDLAVVVLLI 749

Query: 306  ILPVLESQN----LGTESIWPMLAQESLKALGGLGLLSLGGKL-----------ILRRVF 365
            ++P++   +    +G ++I   L   ++KA   +  +  GG+L           +LR ++
Sbjct: 750  LIPLISPNSSKGGIGFQAIAEALGLAAIKAAVAITGIIAGGRLFYNALIFWYTQLLRPIY 809

Query: 366  EVVAEARSSEAFVALCLLTVAGTSLITQKLGFSDTLGAFLAGAILAETNFRTQIEADIRP 425
            + +AE R++E F A  LL + GTSL+T + G S  LGAFLAG +LAET F  Q+E+DI P
Sbjct: 810  KQIAENRNAEIFSANTLLVILGTSLLTARAGLSMALGAFLAGLLLAETEFSLQVESDIAP 869

Query: 426  FRGLLLGLFFVTTGTSIDMQLLFREWPNVLALLAGLIAIKTLIITAIGPRVGLTTQESVR 485
            +RGLLLGLFF+T G SID +LL   +P ++  L  L+  KT+++  IG   G++   +VR
Sbjct: 870  YRGLLLGLFFMTVGMSIDPKLLLANFPLIMGTLGLLLVGKTILVVIIGKLFGISIISAVR 929

Query: 486  IGFLLSQGGEFGFVVFSLANRLGVLPLELNKLLIIIVVLSMALTPLLNEAGRKASEFISE 545
            +G LL+ GGEF FV F  A   G++  +L+ LL ++V +SMALTP L   G    + I+ 
Sbjct: 930  VGLLLAPGGEFAFVAFGEAVNQGIMTPQLSSLLFLVVGISMALTPWLAAGG----QLIAS 989

Query: 546  KYKTEDKAA----DTVNFDATEPVVIVGFGQMGQFFSFKSCFFFVGSGKFLIYTLASGID 605
            +++ +D  +    ++   D    ++I GFG++GQ  +           + L   L     
Sbjct: 990  RFELQDVRSLLPVESETDDLQGHIIICGFGRIGQIIA-----------QLLSERLI---- 1049

Query: 606  GNTPGWPYVAFDIDLSVVKTSRKLGFPVLYGDGSRPAVLQSAGISSPKAVMVMFTEKKAT 665
                  P+VA D+    V   R L  PV +GD     VL   G     A  +      A 
Sbjct: 1050 ------PFVALDVSSDRVAIGRSLDLPVYFGDAGSREVLHKIGADRACAAAIALDTPGAN 1109

Query: 666  IDAVQKLRLAFPAIPIYARAKDVVHLLDLKTAGATDAILEDAETSLQLGSKLL 686
               V  L   FP +  + RA DV H L+L+ AGAT  + E  E SLQL + +L
Sbjct: 1110 YRCVWALSKYFPNVKTFVRAHDVDHGLNLEKAGATAVVPETLEPSLQLAAAVL 1117