IVF0012029 (gene) Melon (IVF77) v1

BLAST of IVF0012029 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 869.8 bits (2246), Expect = 2.3e-251
Identity = 440/752 (58.51%), Postives = 540/752 (71.81%), Query Frame = 0

Query: 18  AEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGF 77
           A K SY+V LGSH+H  +++    + V  SH   L SF+ S E AK++IFYSYK+HINGF
Sbjct: 37  ALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGF 96

Query: 78  AATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKD 137
           AA LD+ +A  +A HP+V +V PNK + L+TTHSW FM L KNGVV  SS W  A +G+D
Sbjct: 97  AAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGED 156

Query: 138 VIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLE 197
            IIANLDTGVWPESKSF + G  G  P++WKG C       VPCN+KLIGA+YFNKGYL 
Sbjct: 157 TIIANLDTGVWPESKSFSDEGY-GAVPARWKGRC----HKDVPCNRKLIGARYFNKGYLA 216

Query: 198 YLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVA 257
           Y     + +  ++   + RD+DGHGSHTLSTAAGN+V GA+VFG G GTA GGSPKARVA
Sbjct: 217 Y-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVA 276

Query: 258 AYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKK 317
           AYKVCWP   G  CFDADI  A + AI DGVDVLS S+GGD   Y  D IAI SFHAVK 
Sbjct: 277 AYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKN 336

Query: 318 GIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTG 377
           G+ VVC+ GNSGP   T SN APW++TVGAS++DREF A V L+NG  F G+S SK L  
Sbjct: 337 GVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPE 396

Query: 378 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 437
            K+Y LI+ A A   N N  DA+LCK  +LD  KVKGKILVCLRG+ AR+DKG QAA AG
Sbjct: 397 EKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAG 456

Query: 438 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 497
           A GM+LCNDK SG+ I+ D H+LPAS I+Y+DG+ L SY++S ++P GY+  P A +NTK
Sbjct: 457 AAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTK 516

Query: 498 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 557
           PAP MA FSSRGPNTI+P I+KPD+TAPGVNIIAAF+EA  PT   SDNR TPF T SGT
Sbjct: 517 PAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGT 576

Query: 558 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 617
           SMSCPH++G+VGLL+ LHP WSP+AI+SAIMT+S+ R+N   PM+D        A PF+Y
Sbjct: 577 SMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE---SFKKANPFSY 636

Query: 618 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEP-FKCPPASSVLNLNY 677
           GSGH+ P  A  PGLVYDL+  DYL+FLCA GY+   ++ F+++P + C   +++L+ NY
Sbjct: 637 GSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNY 696

Query: 678 PSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELT 737
           PSI V NL GS++VTRKLKNVG P  Y A+   P GV VSV+P+ L F + GE K F++T
Sbjct: 697 PSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMT 756

Query: 738 L--AGVVPKDRVGYGALIWSDGRHVVRSPIVV 766
           L    V P   V +G L W+D  H VRSPIVV
Sbjct: 757 LRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774

BLAST of IVF0012029 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 9.9e-226
Identity = 397/754 (52.65%), Postives = 524/754 (69.50%), Query Frame = 0

Query: 19  EKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFA 78
           + +SYVV  G+HSH  E+TE   + V ++H+  LGSF  S E+A D+IFYSY KHINGFA
Sbjct: 28  DSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFA 87

Query: 79  ATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDV 138
           A LD + A  ++ HPEV +V PNK   L+TT SW+F+ LE N  VP SS WR A+FG+D 
Sbjct: 88  AHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDT 147

Query: 139 IIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEY 198
           IIANLDTGVWPESKSF + G+ GP PS+WKG C ++      CN+KLIGA+YFNKGY   
Sbjct: 148 IIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAA 207

Query: 199 LKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAA 258
           +   NS+ D      S RD DGHGSHTLSTAAG++V G S+FG G GTAKGGSP+ARVAA
Sbjct: 208 VGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAA 267

Query: 259 YKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKG 318
           YKVCWP  +G  C+DAD+  AFD AIHDG DV+S+SLGG+P  +  DS+AI SFHA KK 
Sbjct: 268 YKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKR 327

Query: 319 IPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSS-HSKGLTG 378
           I VVC+ GNSGP   T SN APW +TVGAST+DREF + +VL NG  + G S  S  L  
Sbjct: 328 IVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPH 387

Query: 379 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 438
            K YP++    AKA NA+  DA LCK  +LD  K KGKILVCLRG+  R++KG+  AL G
Sbjct: 388 AKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGG 447

Query: 439 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 498
            +GM+L N  ++G+ ++ D H+LPA+ +  +D   +  YI+  + P+ ++ P    +  K
Sbjct: 448 GIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLK 507

Query: 499 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 558
           PAP MA FSS+GP+ ++P+I+KPD+TAPGV++IAA++ A+SPT +  D R   F  +SGT
Sbjct: 508 PAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGT 567

Query: 559 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 618
           SMSCPH++G+ GLL+  +P WSP+AI+SAIMT++ + D+   P+ +  ++    ATPF++
Sbjct: 568 SMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM---KATPFSF 627

Query: 619 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKC-PPASSVLNLNY 678
           G+GH+ P  A++PGLVYDL   DYL FLC+ GY+   I  FS   F C  P  S++NLNY
Sbjct: 628 GAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNY 687

Query: 679 PSIGVQNLKGS-VSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFEL 738
           PSI V NL  S V+V+R +KNVG P +Y  ++ +P GV V+VKP  L F +VGE+K+F++
Sbjct: 688 PSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKV 747

Query: 739 TLA---GVVPKDRVGYGALIWSDGRHVVRSPIVV 766
            L    G V K  V +G L+WSD +H VRSPIVV
Sbjct: 748 ILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of IVF0012029 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 638.3 bits (1645), Expect = 1.1e-181
Identity = 356/772 (46.11%), Postives = 479/772 (62.05%), Query Frame = 0

Query: 20  KNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAA 79
           K  Y+V +G+HSHG   T  D E   DSH+ LLGS   S EKAK++I YSY +HINGFAA
Sbjct: 29  KKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREKAKEAIIYSYNRHINGFAA 88

Query: 80  TLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVI 139
            L++E+A  +A +P V +V  +K   L+TT SWEF+ L + G    +S W+  +FG++ I
Sbjct: 89  LLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG---QNSAWQKGRFGENTI 148

Query: 140 IANLDTGVWPESKSFGEHGIDGPAPSKWKGGC--TDKSPDGV--PCNKKLIGAKYFNKGY 199
           I N+DTGVWPES+SF + G  G  PSKW+GG    +K P  +   CN+KLIGA+Y+NK +
Sbjct: 149 IGNIDTGVWPESQSFSDKGY-GTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAF 208

Query: 200 LEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKAR 259
                 E     L  ++++ RD+ GHG+HTLSTA GN+V GA VF  G GTAKGGSP+AR
Sbjct: 209 ------EAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRAR 268

Query: 260 VAAYKVCWPF-EQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSE----DSIAIAS 319
           VAAYKVCW   +   C+ AD+  A D AI DGVDV+++S G   +  +E    D I+I +
Sbjct: 269 VAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGA 328

Query: 320 FHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSH 379
           FHA+ K I +V + GN GPTP T +N APW+ T+ ASTLDR+F + + + N     G+S 
Sbjct: 329 FHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASL 388

Query: 380 SKGLTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLR-GETARLDKG 439
              L   + + LI    AK  NA   DA LC+  TLD +KV GKI++C R G+   + +G
Sbjct: 389 FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEG 448

Query: 440 KQAALAGAVGMILCNDKLSGSSIVPDFHLL-----PASHINYQDGQVLLSYINSARNPM- 499
            +A  AGA GMIL N   +G ++  + H+      P      +   V  + I    +P+ 
Sbjct: 449 LEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLK 508

Query: 500 -GYLIPPLAKVNT----KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISP 559
            G  I  +++  T    KPAP MA FSSRGPN I P I+KPDVTAPGVNI+AA+SE  S 
Sbjct: 509 TGDTI-KMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASA 568

Query: 560 TRDASDNRT-TPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTL 619
           +    DNR    F  + GTSMSCPH +G+ GLL+  HP WSP+AIKSAIMT++   DNT 
Sbjct: 569 SSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTN 628

Query: 620 NPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAF 679
            P+ D     LA A  FAYGSGH+ P  AI+PGLVYDLS  DYL FLCASGYD++ I A 
Sbjct: 629 RPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISAL 688

Query: 680 S-DEPFKCPPASSVLNLNYPSIGVQNLK-GSVSVTRKLKNVGSPGVYRAQILHPNGVVVS 739
           + +  F C  + SV +LNYPSI + NL+   V++ R + NVG P  Y      PNG  ++
Sbjct: 689 NFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIA 748

Query: 740 VKPRFLKFERVGEEKSFELTL--AGVVPKDRVGYGALIWSDGRHVVRSPIVV 766
           V P  L F ++GE K+F++ +  +    + +  +G L W+DG+H+VRSPI V
Sbjct: 749 VVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

BLAST of IVF0012029 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 575.1 bits (1481), Expect = 1.2e-162
Identity = 332/776 (42.78%), Postives = 459/776 (59.15%), Query Frame = 0

Query: 2   FIVIRQLGTEMTPKKVAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEK 61
           F ++  LG        +++ +Y+V          + +    S  D H     S LRS   
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSLRSISD 70

Query: 62  AKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKN- 121
           + + + Y+Y+  I+GF+  L  E+A  L   P V +VLP    +L+TT +  F+ L+++ 
Sbjct: 71  SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 130

Query: 122 -GVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGC-TDKSPDG 181
             + P +  +       DV++  LDTGVWPESKS+ + G  GP PS WKGGC    +   
Sbjct: 131 ADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTA 190

Query: 182 VPCNKKLIGAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGAS 241
             CN+KLIGA++F +GY    +S    +D S    S RD DGHG+HT STAAG+ V GAS
Sbjct: 191 SLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 250

Query: 242 VFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDP 301
           + G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  
Sbjct: 251 LLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGM 310

Query: 302 IKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVV 361
             Y  D +AI +F A+++GI V C+ GN+GP+  + SN APWI TVGA TLDR+F A  +
Sbjct: 311 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 370

Query: 362 LQNGHRFMGSSHSKG-LTGRKLYPLITGAQAKAGNA-NEDDAMLCKPETLDHSKVKGKIL 421
           L NG  F G S  KG     KL P I      AGNA N  +  LC   TL   KVKGKI+
Sbjct: 371 LGNGKNFTGVSLFKGEALPDKLLPFI-----YAGNASNATNGNLCMTGTLIPEKVKGKIV 430

Query: 422 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYI 481
           +C RG  AR+ KG     AG VGMIL N   +G  +V D HLLPA+ +  + G ++  Y+
Sbjct: 431 MCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYV 490

Query: 482 NSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAI 541
            +  NP   +      V  KP+P +A FSSRGPN+I+P I+KPD+ APGVNI+AA++ A 
Sbjct: 491 TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAA 550

Query: 542 SPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNT 601
            PT  ASD+R   F  +SGTSMSCPHV+GL  LL+++HP+WSP+AI+SA+MT++      
Sbjct: 551 GPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKD 610

Query: 602 LNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRA 661
             P++D  +    P+TPF +G+GH++PT A +PGL+YDL+  DYL FLCA  Y    IR+
Sbjct: 611 GKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS 670

Query: 662 FSDEPFKCPPAS--SVLNLNYPSIGVQ-NLKGSVSVTRKLKNVGSPGVYRAQIL-HPNGV 721
            S   + C P+   SV +LNYPS  V  +  G+   TR + +VG  G Y  ++     GV
Sbjct: 671 VSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGV 730

Query: 722 VVSVKPRFLKFERVGEEKSFELTLA--GVVPKDRVGYGALIWSDGRHVVRSPIVVS 767
            +SV+P  L F+   E+KS+ +T       P     +G++ WSDG+HVV SP+ +S
Sbjct: 731 KISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of IVF0012029 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 8.3e-156
Identity = 325/733 (44.34%), Postives = 446/733 (60.85%), Query Frame = 0

Query: 58  SDEKAKDS-IFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 117
           S E A++S I + Y    +GF+A +  ++A  L NHP V AV  ++ ++L+TT S +F+ 
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 118 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDK-- 177
           L+          W  + +G DVII   DTG+WPE +SF +  + GP P +W+G C     
Sbjct: 109 LQNQ-----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGAR 168

Query: 178 -SPDGVPCNKKLIGAKYFNKG-YLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGN 237
            SP    CN+K+IGA++F KG     +   N TV+      S RD DGHG+HT STAAG 
Sbjct: 169 FSPRN--CNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGR 228

Query: 238 YVFGASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSL 297
           + F AS+ G   G AKG +PKAR+AAYKVCW  +  GC D+DI  AFD A+ DGVDV+S+
Sbjct: 229 HAFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISI 288

Query: 298 SL-GGDPI--KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTL 357
           S+ GGD I   Y  D IAI S+ A  KGI V  + GN GP   + +N APW+ TVGAST+
Sbjct: 289 SIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTI 348

Query: 358 DREFYAPVVLQNGHRFMGSSHSKG--LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLD 417
           DR F A  +L +GHR  G S   G  L GR ++P++       G +    A LC   TLD
Sbjct: 349 DRNFPADAILGDGHRLRGVSLYAGVPLNGR-MFPVV-----YPGKSGMSSASLCMENTLD 408

Query: 418 HSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQ 477
             +V+GKI++C RG + R+ KG     AG VGMIL N   +G  +V D HL+PA  +   
Sbjct: 409 PKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSN 468

Query: 478 DGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVN 537
           +G  + +Y +S  NP+  +      V  KPAP +A FS RGPN +SPEI+KPD+ APGVN
Sbjct: 469 EGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVN 528

Query: 538 IIAAFSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIM 597
           I+AA+++A+ PT   SD R T F  +SGTSM+CPHV+G   LL++ HPDWSP+ I+SA+M
Sbjct: 529 ILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMM 588

Query: 598 TSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCAS 657
           T++ + DN+   +ID  +     ATP+ YGSGH+N   A++PGLVYD++ +DY+ FLC+ 
Sbjct: 589 TTTNLVDNSNRSLIDEST--GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSI 648

Query: 658 GYDEKTIRAFSDEPFKCPPA--SSVLNLNYPSIGV---QNLKGSVS--VTRKLKNVG-SP 717
           GY  KTI+  +  P +CP     S  NLNYPSI      N +G VS  V R   NVG + 
Sbjct: 649 GYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAE 708

Query: 718 GVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLA-----GVVPKDRVGYGALIWSD 767
            VYRA+I  P GV V+VKP  L F    + +S+ +T+       V+ +    +G++ W D
Sbjct: 709 AVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD 759

BLAST of IVF0012029 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1524.6 bits (3946), Expect = 0.0e+00
Identity = 750/757 (99.08%), Postives = 751/757 (99.21%), Query Frame = 0

Query: 14   PKKVAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH 73
            P  +    SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH
Sbjct: 747  PCSLENDGSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH 806

Query: 74   INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK 133
            INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK
Sbjct: 807  INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK 866

Query: 134  FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK 193
            FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK
Sbjct: 867  FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK 926

Query: 194  GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK 253
            GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK
Sbjct: 927  GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK 986

Query: 254  ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA 313
            ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA
Sbjct: 987  ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA 1046

Query: 314  VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG 373
            VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG
Sbjct: 1047 VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG 1106

Query: 374  LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA 433
            LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA
Sbjct: 1107 LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA 1166

Query: 434  LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV 493
            LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV
Sbjct: 1167 LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV 1226

Query: 494  NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM 553
            NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM
Sbjct: 1227 NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM 1286

Query: 554  SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP 613
            SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP
Sbjct: 1287 SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP 1346

Query: 614  FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL 673
            FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL
Sbjct: 1347 FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL 1406

Query: 674  NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE 733
            NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE
Sbjct: 1407 NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE 1466

Query: 734  LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 771
            LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF
Sbjct: 1467 LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of IVF0012029 vs. ExPASy TrEMBL
Match: A0A0A0LYF1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171030 PE=3 SV=1)

HSP 1 Score: 1405.2 bits (3636), Expect = 0.0e+00
Identity = 687/755 (90.99%), Postives = 721/755 (95.50%), Query Frame = 0

Query: 17  VAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 76
           +A K SYVVLLGSHSHGL+ TEKDF+ VVDSHHKLLGSFLRS+EKAKD+IFYSYKK+ING
Sbjct: 21  IATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNING 80

Query: 77  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGK 136
           FAATLDDEDATRLANHPEVAAVLPNK K+LYTTHSWEFMHLEKNGV+PPSSPW  AKFGK
Sbjct: 81  FAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGK 140

Query: 137 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCT-DKSPDGVPCNKKLIGAKYFNKGY 196
           DVIIANLDTGVWPESKSFGEHGI GPAPSKWKGGCT DK+PDGVPCN+KLIGAKYFNKGY
Sbjct: 141 DVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGY 200

Query: 197 LEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKAR 256
            EYLKSENSTVDLSSIINSTRDY+GHGSHTLSTA GNYV GASVFGSGIGTAKGGSPKAR
Sbjct: 201 FEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKAR 260

Query: 257 VAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVK 316
           VAAYKVCWP+E GGCFDADITEAFDHAIHDGVDVLSLSLG D IKYSED+IAIASFHAVK
Sbjct: 261 VAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVK 320

Query: 317 KGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLT 376
           KGIPVVCAVGNSGP PKTASNTAPWILTVGASTLDREFYAPVVL+NG++FMGSSHSKGL 
Sbjct: 321 KGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLR 380

Query: 377 GRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 436
           GR LYPLITGAQAKAGNA EDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA
Sbjct: 381 GRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 440

Query: 437 GAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNT 496
           GAVGMILCNDKLSG+SI PDFH+LPASHINY DGQVLLSY NSAR PMG LIPPLA+VNT
Sbjct: 441 GAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNT 500

Query: 497 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSG 556
           KPAPTMAVFSSRGPNTISPEIIKPDVTAPGV+IIAAFSEAISPTRD SDNRTTPFITMSG
Sbjct: 501 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSG 560

Query: 557 TSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFA 616
           TSMSCPHVAGLVGLLRNLHPDW+PSAIKSAIMTS+QVRDNTLNPM+DGGSL L PATPFA
Sbjct: 561 TSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFA 620

Query: 617 YGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNY 676
           YGSGHINPTGA+DPGLVYDLSPNDYLEFLCASGYDE+TIRAFSDEPFKCP ++SVLNLNY
Sbjct: 621 YGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNY 680

Query: 677 PSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELT 736
           PSIGVQNLK SV++TRKLKNVG+PGVY+AQILHPN V VSVKPRFLKFERVGEEKSFELT
Sbjct: 681 PSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELT 740

Query: 737 LAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 771
           ++GVVPK+R  YGALIWSDGRH VRSPIVVSSGLF
Sbjct: 741 VSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of IVF0012029 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1231.1 bits (3184), Expect = 0.0e+00
Identity = 594/749 (79.31%), Postives = 662/749 (88.38%), Query Frame = 0

Query: 22  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 81
           SY+VLLGSHSHGLEVT++D + V DSHHKLLGS   SDEKA+++IFYSYKK+INGFAA +
Sbjct: 12  SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAIM 71

Query: 82  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 141
           D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GKDVIIA
Sbjct: 72  DEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIA 131

Query: 142 NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 201
           NLDTGVWPESKSFGEHGI GP PSKWKGGCTDK+ D VPCN+KLIGAKYFNKG+L YLKS
Sbjct: 132 NLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLKS 191

Query: 202 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 261
           EN T   + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARVAAYKV
Sbjct: 192 ENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYKV 251

Query: 262 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 321
           CWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P  Y +D IAI++FHAVKKGIPVV
Sbjct: 252 CWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPVV 311

Query: 322 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 381
           C+ GNSGP  +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL G KLYP
Sbjct: 312 CSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKLYP 371

Query: 382 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 441
           LITGA+AKA NA  ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVGMI
Sbjct: 372 LITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMI 431

Query: 442 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 501
           LCND+LSG   + D H+LPASHINY DGQ + SYI + +NPMGYLIPP AKVNTKPAPTM
Sbjct: 432 LCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAPTM 491

Query: 502 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 561
           A FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT +  DNRT PFITMSGTSMSCP
Sbjct: 492 AAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCP 551

Query: 562 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 621
           HV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGS DLAP+TPFAYGSGHI
Sbjct: 552 HVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHI 611

Query: 622 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 681
            PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLNYPSIGVQ
Sbjct: 612 RPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQ 671

Query: 682 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 741
           NL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFELT+ G VP
Sbjct: 672 NLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVP 731

Query: 742 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 771
           +D+V  G LIW+DG+H VRSPIVVSS LF
Sbjct: 732 EDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of IVF0012029 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1224.2 bits (3166), Expect = 0.0e+00
Identity = 593/754 (78.65%), Postives = 661/754 (87.67%), Query Frame = 0

Query: 17  VAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 76
           +A K SY+VLLGSHSHGLEV ++D E V DSHHKLLGS + SDEKA+D+IFYSYK++ING
Sbjct: 26  IAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLLGSIIGSDEKARDAIFYSYKRNING 85

Query: 77  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGK 136
           FAA +D+E+AT+LA HPEVAAVL NK K L+TTHSWEFMHLEKNGV+PPSS WR AK GK
Sbjct: 86  FAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 145

Query: 137 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYL 196
           DVII NLDTGVW ESKSFGEHGI G  PSKWKGGCTDK+PDGV CN+KLIGAKYFNKG+L
Sbjct: 146 DVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGCTDKTPDGVSCNRKLIGAKYFNKGFL 205

Query: 197 EYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARV 256
            YL S+N T   +S+INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARV
Sbjct: 206 AYLNSQNLT---ASVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARV 265

Query: 257 AAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKK 316
           A+YKVCWP E GGCF+ADI EAFDHAIHD VDVLSLSLGG+P  Y +D IAIA+FHAVKK
Sbjct: 266 ASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLSLSLGGEPADYYDDGIAIAAFHAVKK 325

Query: 317 GIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTG 376
           GIPVVC+ GNSGP  +T SNTAPWILTVGASTLDREF APV LQNGH +MGSS SKGL G
Sbjct: 326 GIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDREFQAPVELQNGHSYMGSSLSKGLKG 385

Query: 377 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 436
            KLYPLITGA+AKA NA  + AMLCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAG
Sbjct: 386 DKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAG 445

Query: 437 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 496
           AVGMILCNDKLSG   + D H+LPASHINY DGQ + SYI S +NPMG LIPP AKVNTK
Sbjct: 446 AVGMILCNDKLSGFETIADPHVLPASHINYNDGQAVFSYIKSTKNPMGSLIPPSAKVNTK 505

Query: 497 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 556
           PAP+MA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT +  DNRT PFITMSGT
Sbjct: 506 PAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGT 565

Query: 557 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 616
           SMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGS DLAPATPFAY
Sbjct: 566 SMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPATPFAY 625

Query: 617 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYP 676
           GSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LN NYP
Sbjct: 626 GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNFNYP 685

Query: 677 SIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTL 736
           SIGVQNL GSV++TRKLKNV +PGVY+A+++HPNGV V VKP+ LKFERVGEEK FEL +
Sbjct: 686 SIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNGVKVLVKPKVLKFERVGEEKRFELII 745

Query: 737 AGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 771
            G VP+++V  G LIW+DG+H VRSPIVVSSGLF
Sbjct: 746 TGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of IVF0012029 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 576/755 (76.29%), Postives = 656/755 (86.89%), Query Frame = 0

Query: 17  VAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 76
           +A K SYVV+LGSHSHGLEV+E D + VVDSHHKLLGSFL S EKAKD+IFYSYKK+ING
Sbjct: 25  IAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKKNING 84

Query: 77  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEK-NGVVPPSSPWRMAKFG 136
           FAA L++E+A  LA HPEVAAVL NK K L+TTHSW FM LEK NGVVPP+SPW  A FG
Sbjct: 85  FAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVPPTSPWWRANFG 144

Query: 137 KDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGY 196
           +D IIANLDTGVWPESKSFGE+GI G  P+KWKGGCTD SPD VPCN+KLIGAKYFNKG+
Sbjct: 145 QDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLIGAKYFNKGF 204

Query: 197 LEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKAR 256
           + YLK+ NS+ DLSSI NSTRDY+GHGSHTLSTA G++V  ASVFGSG+GTAKGGSPKAR
Sbjct: 205 IAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGLGTAKGGSPKAR 264

Query: 257 VAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVK 316
           VAAYK+CWPF+ GGCFDADITE FDHAIHDGV+V+SLS+GG P  Y +DSIAIA+FHAVK
Sbjct: 265 VAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDSIAIAAFHAVK 324

Query: 317 KGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLT 376
           KGIPVVC+ GNSGP   TASNTAPWILTVGASTLDR+F APV L+NG RF GSS S  L 
Sbjct: 325 KGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRFQGSSLSTALP 384

Query: 377 GRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 436
             KLYPLITGAQAKA NA+  DAMLCKPETLDHSK KGKIL CLRG+ AR+DKG+QAALA
Sbjct: 385 EDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNARVDKGEQAALA 444

Query: 437 GAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNT 496
           GA GMILCND+LSG   + D HLLPASH+NY DGQ +L YI S +NPMGYLIPP AK+NT
Sbjct: 445 GAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGYLIPPAAKLNT 504

Query: 497 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSG 556
           KPAP MA FSSRGPN ++PEIIKPDVTAPGVN+IAA++EA+SPT +  DNRT PFITMSG
Sbjct: 505 KPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSG 564

Query: 557 TSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFA 616
           TSMSCPHVAGL GLLR+LHP+WSPSAIKSAIMTS+++RDNT+ PM+DGG+ +LAPATPF+
Sbjct: 565 TSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFS 624

Query: 617 YGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNY 676
           YGSGHI P GA+DPGLVYDL+P+DYLEFLCA GYDEK IRAFSD P+KCPP++S+LN NY
Sbjct: 625 YGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNY 684

Query: 677 PSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELT 736
           PSIGVQN+ GSV+VTR+LKNVG+PGVYRA++  P GV VSV+PRFLKF++VGEEKSF+LT
Sbjct: 685 PSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLT 744

Query: 737 LAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 771
           +AGVVP  RV  G LIW+DG H VRSPIV+SSGLF
Sbjct: 745 IAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of IVF0012029 vs. NCBI nr
Match: TYK15479.1 (subtilisin-like protease SBT5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 1519 bits (3933), Expect = 0.0
Identity = 750/757 (99.08%), Postives = 751/757 (99.21%), Query Frame = 0

Query: 14   PKKVAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH 73
            P  +    SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH
Sbjct: 747  PCSLENDGSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKH 806

Query: 74   INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK 133
            INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK
Sbjct: 807  INGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAK 866

Query: 134  FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK 193
            FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK
Sbjct: 867  FGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNK 926

Query: 194  GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK 253
            GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK
Sbjct: 927  GYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPK 986

Query: 254  ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA 313
            ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA
Sbjct: 987  ARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHA 1046

Query: 314  VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG 373
            VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG
Sbjct: 1047 VKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKG 1106

Query: 374  LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA 433
            LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA
Sbjct: 1107 LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAA 1166

Query: 434  LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV 493
            LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV
Sbjct: 1167 LAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKV 1226

Query: 494  NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM 553
            NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM
Sbjct: 1227 NTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITM 1286

Query: 554  SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP 613
            SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP
Sbjct: 1287 SGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATP 1346

Query: 614  FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL 673
            FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL
Sbjct: 1347 FAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNL 1406

Query: 674  NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE 733
            NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE
Sbjct: 1407 NYPSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFE 1466

Query: 734  LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 770
            LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF
Sbjct: 1467 LTLAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of IVF0012029 vs. NCBI nr
Match: XP_011648463.1 (subtilisin-like protease SBT5.4 [Cucumis sativus] >KGN64981.1 hypothetical protein Csa_022745 [Cucumis sativus])

HSP 1 Score: 1400 bits (3623), Expect = 0.0
Identity = 687/755 (90.99%), Postives = 721/755 (95.50%), Query Frame = 0

Query: 17  VAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 76
           +A K SYVVLLGSHSHGL+ TEKDF+ VVDSHHKLLGSFLRS+EKAKD+IFYSYKK+ING
Sbjct: 21  IATKKSYVVLLGSHSHGLDATEKDFKRVVDSHHKLLGSFLRSEEKAKDAIFYSYKKNING 80

Query: 77  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGK 136
           FAATLDDEDATRLANHPEVAAVLPNK K+LYTTHSWEFMHLEKNGV+PPSSPW  AKFGK
Sbjct: 81  FAATLDDEDATRLANHPEVAAVLPNKAKNLYTTHSWEFMHLEKNGVIPPSSPWWRAKFGK 140

Query: 137 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTD-KSPDGVPCNKKLIGAKYFNKGY 196
           DVIIANLDTGVWPESKSFGEHGI GPAPSKWKGGCTD K+PDGVPCN+KLIGAKYFNKGY
Sbjct: 141 DVIIANLDTGVWPESKSFGEHGIVGPAPSKWKGGCTDDKTPDGVPCNQKLIGAKYFNKGY 200

Query: 197 LEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKAR 256
            EYLKSENSTVDLSSIINSTRDY+GHGSHTLSTA GNYV GASVFGSGIGTAKGGSPKAR
Sbjct: 201 FEYLKSENSTVDLSSIINSTRDYNGHGSHTLSTAGGNYVVGASVFGSGIGTAKGGSPKAR 260

Query: 257 VAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVK 316
           VAAYKVCWP+E GGCFDADITEAFDHAIHDGVDVLSLSLG D IKYSED+IAIASFHAVK
Sbjct: 261 VAAYKVCWPYEHGGCFDADITEAFDHAIHDGVDVLSLSLGSDAIKYSEDAIAIASFHAVK 320

Query: 317 KGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLT 376
           KGIPVVCAVGNSGP PKTASNTAPWILTVGASTLDREFYAPVVL+NG++FMGSSHSKGL 
Sbjct: 321 KGIPVVCAVGNSGPLPKTASNTAPWILTVGASTLDREFYAPVVLRNGYKFMGSSHSKGLR 380

Query: 377 GRKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 436
           GR LYPLITGAQAKAGNA EDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA
Sbjct: 381 GRNLYPLITGAQAKAGNATEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALA 440

Query: 437 GAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNT 496
           GAVGMILCNDKLSG+SI PDFH+LPASHINY DGQVLLSY NSAR PMG LIPPLA+VNT
Sbjct: 441 GAVGMILCNDKLSGTSINPDFHVLPASHINYHDGQVLLSYTNSARYPMGCLIPPLARVNT 500

Query: 497 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSG 556
           KPAPTMAVFSSRGPNTISPEIIKPDVTAPGV+IIAAFSEAISPTRD SDNRTTPFITMSG
Sbjct: 501 KPAPTMAVFSSRGPNTISPEIIKPDVTAPGVDIIAAFSEAISPTRDPSDNRTTPFITMSG 560

Query: 557 TSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFA 616
           TSMSCPHVAGLVGLLRNLHPDW+PSAIKSAIMTS+QVRDNTLNPM+DGGSL L PATPFA
Sbjct: 561 TSMSCPHVAGLVGLLRNLHPDWTPSAIKSAIMTSAQVRDNTLNPMLDGGSLGLDPATPFA 620

Query: 617 YGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNY 676
           YGSGHINPTGA+DPGLVYDLSPNDYLEFLCASGYDE+TIRAFSDEPFKCP ++SVLNLNY
Sbjct: 621 YGSGHINPTGAVDPGLVYDLSPNDYLEFLCASGYDERTIRAFSDEPFKCPASASVLNLNY 680

Query: 677 PSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELT 736
           PSIGVQNLK SV++TRKLKNVG+PGVY+AQILHPN V VSVKPRFLKFERVGEEKSFELT
Sbjct: 681 PSIGVQNLKDSVTITRKLKNVGTPGVYKAQILHPNVVQVSVKPRFLKFERVGEEKSFELT 740

Query: 737 LAGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 770
           ++GVVPK+R  YGALIWSDGRH VRSPIVVSSGLF
Sbjct: 741 VSGVVPKNRFAYGALIWSDGRHFVRSPIVVSSGLF 775

BLAST of IVF0012029 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1268 bits (3282), Expect = 0.0
Identity = 616/754 (81.70%), Postives = 678/754 (89.92%), Query Frame = 0

Query: 17  VAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 76
           +A K SYVVLLGSHSHG+E+TE+D E VV SHHKLLGSFL S+EKAKD+IFYSYKKHING
Sbjct: 21  IAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLLGSFLGSEEKAKDAIFYSYKKHING 80

Query: 77  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGK 136
           FAATLD+E+AT+LA HPEVAA+L N+ K L+TTHSWEFMHLEKNGV+P SSPWR+AK GK
Sbjct: 81  FAATLDEEEATKLAEHPEVAAILANRAKALHTTHSWEFMHLEKNGVIPSSSPWRIAKSGK 140

Query: 137 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYL 196
           DVIIANLDTGVWPESKSFGEHGI GP PSKWKGGC D++PD VPCN+KLIGAKYFNKG++
Sbjct: 141 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCMDETPDRVPCNRKLIGAKYFNKGFI 200

Query: 197 EYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARV 256
            YLKSENST + SS+INSTRDY+GHGSHTLSTA G+YV GASVFGSG GTAKGGSPKARV
Sbjct: 201 AYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGGSYVSGASVFGSGAGTAKGGSPKARV 260

Query: 257 AAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKK 316
           AAYKVCWP E GGCFD+DI EAFDHAIHDGVDVLSLSLG DP +YSEDSIAIA+FHAVKK
Sbjct: 261 AAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLSLSLGSDPAEYSEDSIAIAAFHAVKK 320

Query: 317 GIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTG 376
           GIPVVC+ GNSGP   TASNTAPWILTVGASTLDREF APV LQNGHR+MGSS SK L G
Sbjct: 321 GIPVVCSAGNSGPDALTASNTAPWILTVGASTLDREFQAPVELQNGHRYMGSSLSKALQG 380

Query: 377 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 436
            KLYPLITGAQAKA NA  DDAMLCKPETLDHSKVKGKIL CLRG+ AR+DKG+QAALAG
Sbjct: 381 NKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKVKGKILACLRGDNARVDKGEQAALAG 440

Query: 437 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 496
           AVGMILCND+LSG   + D H+LPASHINY DGQ +LSYINS +NPMGYLIPP AKVNTK
Sbjct: 441 AVGMILCNDELSGFETIADPHVLPASHINYNDGQAVLSYINSTKNPMGYLIPPSAKVNTK 500

Query: 497 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 556
           PAPTMA FSSRGPN ISPEIIKPDVTAPGVN+IAAFSEA+SPT +A DNRT PFITMSGT
Sbjct: 501 PAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAAFSEAVSPTGEAFDNRTVPFITMSGT 560

Query: 557 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 616
           SMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT+ PM+DGGS DLAPATPFAY
Sbjct: 561 SMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSARIRDNTMKPMLDGGSPDLAPATPFAY 620

Query: 617 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYP 676
           GSGHI PTGAIDPGLVYDLSPNDYLEFLCASGYD+KTIRAFSD PFKCPP +S+LN NYP
Sbjct: 621 GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDDKTIRAFSDGPFKCPPNASILNFNYP 680

Query: 677 SIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTL 736
           SIGVQNL G+V+VTRKLKNV +PGVYRA+I++P+GV VSVKP+ LKFERV EEKSFELT+
Sbjct: 681 SIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDGVKVSVKPKVLKFERVAEEKSFELTM 740

Query: 737 AGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 770
            G VP+D+V  G LIW+DG+H VRSPIV+SS LF
Sbjct: 741 TGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773

BLAST of IVF0012029 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 1228 bits (3176), Expect = 0.0
Identity = 597/754 (79.18%), Postives = 666/754 (88.33%), Query Frame = 0

Query: 18  AEKN-SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHING 77
           A+KN SY+VLLGSHSHGLEVT++D + V DSHHKLLGS   SDEKA+++IFYSYKK+ING
Sbjct: 3   AKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNING 62

Query: 78  FAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGK 137
           FAA +D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GK
Sbjct: 63  FAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGK 122

Query: 138 DVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYL 197
           DVIIANLDTGVWPESKSFGEHGI GP PSKWKGGCTDK+ D VPCN+KLIGAKYFNKG+L
Sbjct: 123 DVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFL 182

Query: 198 EYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARV 257
            YLKSEN T   + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARV
Sbjct: 183 AYLKSENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARV 242

Query: 258 AAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKK 317
           AAYKVCWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P  Y +D IAI++FHAVKK
Sbjct: 243 AAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKK 302

Query: 318 GIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTG 377
           GIPVVC+ GNSGP  +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL G
Sbjct: 303 GIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKG 362

Query: 378 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 437
            KLYPLITGA+AKA NA  ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAG
Sbjct: 363 DKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAG 422

Query: 438 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 497
           AVGMILCND+LSG   + D H+LPASHINY DGQ + SYI + +NPMGYLIPP AKVNTK
Sbjct: 423 AVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTK 482

Query: 498 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 557
           PAPTMA FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT +  DNRT PFITMSGT
Sbjct: 483 PAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGT 542

Query: 558 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 617
           SMSCPHV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGS DLAP+TPFAY
Sbjct: 543 SMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAY 602

Query: 618 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYP 677
           GSGHI PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLNYP
Sbjct: 603 GSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYP 662

Query: 678 SIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTL 737
           SIGVQNL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFELT+
Sbjct: 663 SIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTI 722

Query: 738 AGVVPKDRVGYGALIWSDGRHVVRSPIVVSSGLF 770
            G VP+D+V  G LIW+DG+H VRSPIVVSS LF
Sbjct: 723 TGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753

BLAST of IVF0012029 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1226 bits (3172), Expect = 0.0
Identity = 594/749 (79.31%), Postives = 662/749 (88.38%), Query Frame = 0

Query: 22  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 81
           SY+VLLGSHSHGLEVT++D + V DSHHKLLGS   SDEKA+++IFYSYKK+INGFAA +
Sbjct: 12  SYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNINGFAAIM 71

Query: 82  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDVIIA 141
           D+E+A +LA HPEVAAVLPN+ K L+TTHSWEFMHLEKNGV+PPSS WR AK GKDVIIA
Sbjct: 72  DEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEKNGVIPPSSAWRRAKSGKDVIIA 131

Query: 142 NLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLKS 201
           NLDTGVWPESKSFGEHGI GP PSKWKGGCTDK+ D VPCN+KLIGAKYFNKG+L YLKS
Sbjct: 132 NLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNKGFLAYLKS 191

Query: 202 ENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKV 261
           EN T   + +INSTRDYDGHGSHTLSTA G+YV GASVFG G+GTAKGGSPKARVAAYKV
Sbjct: 192 ENLT---ALVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPKARVAAYKV 251

Query: 262 CWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVV 321
           CWP E GGCFDADI +AFDHAIHD VDVLSLSLGG+P  Y +D IAI++FHAVKKGIPVV
Sbjct: 252 CWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHAVKKGIPVV 311

Query: 322 CAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLYP 381
           C+ GNSGP  +T SNTAPWILTVGAST+DREF APV LQNGHR+MGSS SKGL G KLYP
Sbjct: 312 CSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKGLKGDKLYP 371

Query: 382 LITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMI 441
           LITGA+AKA NA  ++A LCKP+TLDHSKVKGKILVCLRG+TAR+DKG+QAALAGAVGMI
Sbjct: 372 LITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAALAGAVGMI 431

Query: 442 LCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTM 501
           LCND+LSG   + D H+LPASHINY DGQ + SYI + +NPMGYLIPP AKVNTKPAPTM
Sbjct: 432 LCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKVNTKPAPTM 491

Query: 502 AVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCP 561
           A FSSRGPN ISPEIIKPDVTAPGVNIIAAFSEA+SPT +  DNRT PFITMSGTSMSCP
Sbjct: 492 AAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITMSGTSMSCP 551

Query: 562 HVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHI 621
           HV+GLVGLLR LHP WSPSAIKSAIMTS+++RDNT  PM+DGGS DLAP+TPFAYGSGHI
Sbjct: 552 HVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTPFAYGSGHI 611

Query: 622 NPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGVQ 681
            PTGAIDPGLVYDLSPNDYLEFLCASGY+EKTI+AFSD PFKCP ++S+LNLNYPSIGVQ
Sbjct: 612 RPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNLNYPSIGVQ 671

Query: 682 NLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVVP 741
           NL GSV+VTRKLKNV +PGVY+ ++ HPNGV V VKP+ LKFERVGEEKSFELT+ G VP
Sbjct: 672 NLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFELTITGDVP 731

Query: 742 KDRVGYGALIWSDGRHVVRSPIVVSSGLF 770
           +D+V  G LIW+DG+H VRSPIVVSS LF
Sbjct: 732 EDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of IVF0012029 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 869.8 bits (2246), Expect = 1.7e-252
Identity = 440/752 (58.51%), Postives = 540/752 (71.81%), Query Frame = 0

Query: 18  AEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGF 77
           A K SY+V LGSH+H  +++    + V  SH   L SF+ S E AK++IFYSYK+HINGF
Sbjct: 37  ALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHENAKEAIFYSYKRHINGF 96

Query: 78  AATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKD 137
           AA LD+ +A  +A HP+V +V PNK + L+TTHSW FM L KNGVV  SS W  A +G+D
Sbjct: 97  AAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKNGVVHKSSLWNKAGYGED 156

Query: 138 VIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLE 197
            IIANLDTGVWPESKSF + G  G  P++WKG C       VPCN+KLIGA+YFNKGYL 
Sbjct: 157 TIIANLDTGVWPESKSFSDEGY-GAVPARWKGRC----HKDVPCNRKLIGARYFNKGYLA 216

Query: 198 YLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVA 257
           Y     + +  ++   + RD+DGHGSHTLSTAAGN+V GA+VFG G GTA GGSPKARVA
Sbjct: 217 Y-----TGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVA 276

Query: 258 AYKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKK 317
           AYKVCWP   G  CFDADI  A + AI DGVDVLS S+GGD   Y  D IAI SFHAVK 
Sbjct: 277 AYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKN 336

Query: 318 GIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTG 377
           G+ VVC+ GNSGP   T SN APW++TVGAS++DREF A V L+NG  F G+S SK L  
Sbjct: 337 GVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSKPLPE 396

Query: 378 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 437
            K+Y LI+ A A   N N  DA+LCK  +LD  KVKGKILVCLRG+ AR+DKG QAA AG
Sbjct: 397 EKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAG 456

Query: 438 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 497
           A GM+LCNDK SG+ I+ D H+LPAS I+Y+DG+ L SY++S ++P GY+  P A +NTK
Sbjct: 457 AAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTK 516

Query: 498 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 557
           PAP MA FSSRGPNTI+P I+KPD+TAPGVNIIAAF+EA  PT   SDNR TPF T SGT
Sbjct: 517 PAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGT 576

Query: 558 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 617
           SMSCPH++G+VGLL+ LHP WSP+AI+SAIMT+S+ R+N   PM+D        A PF+Y
Sbjct: 577 SMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDE---SFKKANPFSY 636

Query: 618 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEP-FKCPPASSVLNLNY 677
           GSGH+ P  A  PGLVYDL+  DYL+FLCA GY+   ++ F+++P + C   +++L+ NY
Sbjct: 637 GSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNY 696

Query: 678 PSIGVQNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELT 737
           PSI V NL GS++VTRKLKNVG P  Y A+   P GV VSV+P+ L F + GE K F++T
Sbjct: 697 PSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQMT 756

Query: 738 L--AGVVPKDRVGYGALIWSDGRHVVRSPIVV 766
           L    V P   V +G L W+D  H VRSPIVV
Sbjct: 757 LRPLPVTPSGYV-FGELTWTDSHHYVRSPIVV 774

BLAST of IVF0012029 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 784.6 bits (2025), Expect = 7.0e-227
Identity = 397/754 (52.65%), Postives = 524/754 (69.50%), Query Frame = 0

Query: 19  EKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFA 78
           + +SYVV  G+HSH  E+TE   + V ++H+  LGSF  S E+A D+IFYSY KHINGFA
Sbjct: 28  DSSSYVVYFGAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERATDAIFYSYTKHINGFA 87

Query: 79  ATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKNGVVPPSSPWRMAKFGKDV 138
           A LD + A  ++ HPEV +V PNK   L+TT SW+F+ LE N  VP SS WR A+FG+D 
Sbjct: 88  AHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVPSSSIWRKARFGEDT 147

Query: 139 IIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEY 198
           IIANLDTGVWPESKSF + G+ GP PS+WKG C ++      CN+KLIGA+YFNKGY   
Sbjct: 148 IIANLDTGVWPESKSFRDEGL-GPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAA 207

Query: 199 LKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAA 258
           +   NS+ D      S RD DGHGSHTLSTAAG++V G S+FG G GTAKGGSP+ARVAA
Sbjct: 208 VGHLNSSFD------SPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAA 267

Query: 259 YKVCWPFEQGG-CFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKG 318
           YKVCWP  +G  C+DAD+  AFD AIHDG DV+S+SLGG+P  +  DS+AI SFHA KK 
Sbjct: 268 YKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKR 327

Query: 319 IPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSS-HSKGLTG 378
           I VVC+ GNSGP   T SN APW +TVGAST+DREF + +VL NG  + G S  S  L  
Sbjct: 328 IVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPH 387

Query: 379 RKLYPLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAG 438
            K YP++    AKA NA+  DA LCK  +LD  K KGKILVCLRG+  R++KG+  AL G
Sbjct: 388 AKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGG 447

Query: 439 AVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTK 498
            +GM+L N  ++G+ ++ D H+LPA+ +  +D   +  YI+  + P+ ++ P    +  K
Sbjct: 448 GIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLK 507

Query: 499 PAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGT 558
           PAP MA FSS+GP+ ++P+I+KPD+TAPGV++IAA++ A+SPT +  D R   F  +SGT
Sbjct: 508 PAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGT 567

Query: 559 SMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAY 618
           SMSCPH++G+ GLL+  +P WSP+AI+SAIMT++ + D+   P+ +  ++    ATPF++
Sbjct: 568 SMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNM---KATPFSF 627

Query: 619 GSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKC-PPASSVLNLNY 678
           G+GH+ P  A++PGLVYDL   DYL FLC+ GY+   I  FS   F C  P  S++NLNY
Sbjct: 628 GAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNY 687

Query: 679 PSIGVQNLKGS-VSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFEL 738
           PSI V NL  S V+V+R +KNVG P +Y  ++ +P GV V+VKP  L F +VGE+K+F++
Sbjct: 688 PSITVPNLTSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKV 747

Query: 739 TLA---GVVPKDRVGYGALIWSDGRHVVRSPIVV 766
            L    G V K  V +G L+WSD +H VRSPIVV
Sbjct: 748 ILVKSKGNVAKGYV-FGELVWSDKKHRVRSPIVV 770

BLAST of IVF0012029 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 575.1 bits (1481), Expect = 8.5e-164
Identity = 332/776 (42.78%), Postives = 459/776 (59.15%), Query Frame = 0

Query: 2   FIVIRQLGTEMTPKKVAEKNSYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEK 61
           F ++  LG        +++ +Y+V          + +    S  D H     S LRS   
Sbjct: 11  FFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSLRSISD 70

Query: 62  AKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEKN- 121
           + + + Y+Y+  I+GF+  L  E+A  L   P V +VLP    +L+TT +  F+ L+++ 
Sbjct: 71  SAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHT 130

Query: 122 -GVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGC-TDKSPDG 181
             + P +  +       DV++  LDTGVWPESKS+ + G  GP PS WKGGC    +   
Sbjct: 131 ADLFPEAGSY------SDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTNFTA 190

Query: 182 VPCNKKLIGAKYFNKGYLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGAS 241
             CN+KLIGA++F +GY    +S    +D S    S RD DGHG+HT STAAG+ V GAS
Sbjct: 191 SLCNRKLIGARFFARGY----ESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGAS 250

Query: 242 VFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDP 301
           + G   GTA+G +P+ARVA YKVCW    GGCF +DI  A D AI D V+VLS+SLGG  
Sbjct: 251 LLGYASGTARGMAPRARVAVYKVCW---LGGCFSSDILAAIDKAIADNVNVLSMSLGGGM 310

Query: 302 IKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVV 361
             Y  D +AI +F A+++GI V C+ GN+GP+  + SN APWI TVGA TLDR+F A  +
Sbjct: 311 SDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAI 370

Query: 362 LQNGHRFMGSSHSKG-LTGRKLYPLITGAQAKAGNA-NEDDAMLCKPETLDHSKVKGKIL 421
           L NG  F G S  KG     KL P I      AGNA N  +  LC   TL   KVKGKI+
Sbjct: 371 LGNGKNFTGVSLFKGEALPDKLLPFI-----YAGNASNATNGNLCMTGTLIPEKVKGKIV 430

Query: 422 VCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYI 481
           +C RG  AR+ KG     AG VGMIL N   +G  +V D HLLPA+ +  + G ++  Y+
Sbjct: 431 MCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYV 490

Query: 482 NSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAI 541
            +  NP   +      V  KP+P +A FSSRGPN+I+P I+KPD+ APGVNI+AA++ A 
Sbjct: 491 TTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAA 550

Query: 542 SPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNT 601
            PT  ASD+R   F  +SGTSMSCPHV+GL  LL+++HP+WSP+AI+SA+MT++      
Sbjct: 551 GPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKD 610

Query: 602 LNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRA 661
             P++D  +    P+TPF +G+GH++PT A +PGL+YDL+  DYL FLCA  Y    IR+
Sbjct: 611 GKPLLDIAT--GKPSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRS 670

Query: 662 FSDEPFKCPPAS--SVLNLNYPSIGVQ-NLKGSVSVTRKLKNVGSPGVYRAQIL-HPNGV 721
            S   + C P+   SV +LNYPS  V  +  G+   TR + +VG  G Y  ++     GV
Sbjct: 671 VSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGV 730

Query: 722 VVSVKPRFLKFERVGEEKSFELTLA--GVVPKDRVGYGALIWSDGRHVVRSPIVVS 767
            +SV+P  L F+   E+KS+ +T       P     +G++ WSDG+HVV SP+ +S
Sbjct: 731 KISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of IVF0012029 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 552.4 bits (1422), Expect = 5.9e-157
Identity = 325/733 (44.34%), Postives = 446/733 (60.85%), Query Frame = 0

Query: 58  SDEKAKDS-IFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMH 117
           S E A++S I + Y    +GF+A +  ++A  L NHP V AV  ++ ++L+TT S +F+ 
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 118 LEKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDK-- 177
           L+          W  + +G DVII   DTG+WPE +SF +  + GP P +W+G C     
Sbjct: 109 LQNQ-----KGLWSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGAR 168

Query: 178 -SPDGVPCNKKLIGAKYFNKG-YLEYLKSENSTVDLSSIINSTRDYDGHGSHTLSTAAGN 237
            SP    CN+K+IGA++F KG     +   N TV+      S RD DGHG+HT STAAG 
Sbjct: 169 FSPRN--CNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAGR 228

Query: 238 YVFGASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITEAFDHAIHDGVDVLSL 297
           + F AS+ G   G AKG +PKAR+AAYKVCW  +  GC D+DI  AFD A+ DGVDV+S+
Sbjct: 229 HAFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVISI 288

Query: 298 SL-GGDPI--KYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNTAPWILTVGASTL 357
           S+ GGD I   Y  D IAI S+ A  KGI V  + GN GP   + +N APW+ TVGAST+
Sbjct: 289 SIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTI 348

Query: 358 DREFYAPVVLQNGHRFMGSSHSKG--LTGRKLYPLITGAQAKAGNANEDDAMLCKPETLD 417
           DR F A  +L +GHR  G S   G  L GR ++P++       G +    A LC   TLD
Sbjct: 349 DRNFPADAILGDGHRLRGVSLYAGVPLNGR-MFPVV-----YPGKSGMSSASLCMENTLD 408

Query: 418 HSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPDFHLLPASHINYQ 477
             +V+GKI++C RG + R+ KG     AG VGMIL N   +G  +V D HL+PA  +   
Sbjct: 409 PKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSN 468

Query: 478 DGQVLLSYINSARNPMGYLIPPLAKVNTKPAPTMAVFSSRGPNTISPEIIKPDVTAPGVN 537
           +G  + +Y +S  NP+  +      V  KPAP +A FS RGPN +SPEI+KPD+ APGVN
Sbjct: 469 EGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVN 528

Query: 538 IIAAFSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGLLRNLHPDWSPSAIKSAIM 597
           I+AA+++A+ PT   SD R T F  +SGTSM+CPHV+G   LL++ HPDWSP+ I+SA+M
Sbjct: 529 ILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMM 588

Query: 598 TSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDPGLVYDLSPNDYLEFLCAS 657
           T++ + DN+   +ID  +     ATP+ YGSGH+N   A++PGLVYD++ +DY+ FLC+ 
Sbjct: 589 TTTNLVDNSNRSLIDEST--GKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSI 648

Query: 658 GYDEKTIRAFSDEPFKCPPA--SSVLNLNYPSIGV---QNLKGSVS--VTRKLKNVG-SP 717
           GY  KTI+  +  P +CP     S  NLNYPSI      N +G VS  V R   NVG + 
Sbjct: 649 GYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAE 708

Query: 718 GVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLA-----GVVPKDRVGYGALIWSD 767
            VYRA+I  P GV V+VKP  L F    + +S+ +T+       V+ +    +G++ W D
Sbjct: 709 AVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD 759

BLAST of IVF0012029 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 547.4 bits (1409), Expect = 1.9e-155
Identity = 315/751 (41.94%), Postives = 443/751 (58.99%), Query Frame = 0

Query: 43  SVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATLDDEDATRLANHPEVAAVLPNK 102
           S+  +H     S L S   +  SI ++Y    +GF+A L  +DA++L +HP V +V+P +
Sbjct: 39  SIFPTHFHWYTSSLASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQ 98

Query: 103 PKDLYTTHSWEFMHL---EKNGVVPPSSPWRMAKFGKDVIIANLDTGVWPESKSFGEHGI 162
            + L+TT S EF+ L   +K G++  S       FG D++I  +DTGVWPE  SF + G+
Sbjct: 99  VRHLHTTRSPEFLGLRSTDKAGLLEESD------FGSDLVIGVIDTGVWPERPSFDDRGL 158

Query: 163 DGPAPSKWKGGC--TDKSPDGVPCNKKLIGAKYFNKGYLEYLKSENSTVDLSSIINSTRD 222
            GP P KWKG C  +   P+   CN+KL+GA++F  GY    ++ N  ++ ++   S RD
Sbjct: 159 -GPVPIKWKGQCIASQDFPESA-CNRKLVGARFFCGGY----EATNGKMNETTEFRSPRD 218

Query: 223 YDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYKVCWPFEQGGCFDADITE 282
            DGHG+HT S +AG YVF AS  G   G A G +PKAR+AAYKVCW     GC+D+DI  
Sbjct: 219 SDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCW---NSGCYDSDILA 278

Query: 283 AFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPVVCAVGNSGPTPKTASNT 342
           AFD A+ DGVDV+SLS+GG  + Y  D+IAI +F A+ +GI V  + GN GP   T +N 
Sbjct: 279 AFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNV 338

Query: 343 APWILTVGASTLDREFYAPVVLQNGHRFMGSS--HSKGLTGRKLYPLITGAQAKAGNANE 402
           APW+ TVGA T+DR+F A V L NG    G S     GL   ++YPL+ G     G+   
Sbjct: 339 APWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGY- 398

Query: 403 DDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGMILCNDKLSGSSIVPD 462
             + LC   +LD + VKGKI++C RG  +R  KG+     G +GMI+ N    G  +V D
Sbjct: 399 -SSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVAD 458

Query: 463 FHLLPASHINYQDGQVLLSYIN------SARNPMGYLIPPLAKVNTKPAPTMAVFSSRGP 522
            H+LPA+ +    G  +  YI+      S+++P   ++    ++  +PAP +A FS+RGP
Sbjct: 459 CHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGP 518

Query: 523 NTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSCPHVAGLVGL 582
           N  +PEI+KPDV APG+NI+AA+ + I P+   SDNR T F  +SGTSM+CPHV+GL  L
Sbjct: 519 NPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAAL 578

Query: 583 LRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGHINPTGAIDP 642
           L+  HPDWSP+AI+SA++T++   DN+  PM+D  + +   ++   YGSGH++PT A+DP
Sbjct: 579 LKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGN--TSSVMDYGSGHVHPTKAMDP 638

Query: 643 GLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCP---PASSVLNLNYPSIGV-----Q 702
           GLVYD++  DY+ FLC S Y    I   +     C     A  V NLNYPS  V      
Sbjct: 639 GLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYG 698

Query: 703 NLKGSVSVTRKLKNVG-SPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVV 762
             K S    R + NVG S  VY  +I  P G  V+V+P  L F RVG++ SF + +    
Sbjct: 699 ESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTE 758

Query: 763 PK-----DRVGYGALIWSDGRHVVRSPIVVS 767
            K       V  G ++WSDG+  V SP+VV+
Sbjct: 759 VKLSPGATNVETGHIVWSDGKRNVTSPLVVT 770