IVF0010169 (gene) Melon (IVF77) v1

Overview
NameIVF0010169
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionProcollagen-proline 4-dioxygenase
Locationtig00000060: 92709 .. 95936 (+)
RNA-Seq ExpressionIVF0010169
SyntenyIVF0010169
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGAGACTTTAGAAAATTTGACAGTTAACAGCTTGAAGAAGAGATGGAAATCTGAAAATTTTGTGAAGAAAAAGAAAAATTTTATTGCATTTTTCTTCTTCTTCTTCATTTCTTCTCTTTCTCTTTTGGTCCGATTCAGTTCCATGGCTGAATTTCTCCGTTTCAATCTACTTTTTCTTTTCACATTAACCATTTCCTACCTTCTCCGGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCCTGGTCTCCTCGGTACTTCAATTTCTCCCTCTTCTTCTTCTTCTTCTTCTTCTACTACTACTACTACTAAGTTTTGATGATCGATTAGATTTGTTCACGGAACCCTGTTCCCGTTTCAATTTGAATGATTCTTCAGGGCTTTTGTGTATGAAGGTTTTCTCACGGATTTAGAATGCGATCATCTCATTTCCCTTGTGAGTAGAATTAGAATCGATTCGTTTTGCTTTTTTCGATGTGCGATTTTTGTTTTGTTTTGTTTTGGTTGATTTTGGTTTTGTTTGAATTAGGCTAAAGCGGAGCTGAAGAGATCCTCTGTTGCGGATAATTTGTCCGGAGAGAGCAAAGTCAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGTTTGTTTAGACTTTAGAGTTTGCTGCTTATAGTTCTGAAGTTTCCAATAAGAGAACCATTGTGGCTTTATATATCATCTTTTAGTTTGAATTACTATCAAACCTAAATCTTGAACTCTCGAGCTTGAAATGCATTGGTTGGATATACCATTGAGATTTCCTTATGGGTTTAAAGATTAGTTTGGTTCATGTTCATGAGATCCATCTTTTGAATATTGGTCTGCTTTATGAAGTAAATTTTCAGATGAAATGAGTGATGAGCAAGTATATGACATTGCAGTAATTTGATCTTCAGATCTTCCATTTTGTGATACTGGTATAAATCCTCATTTGTGAAAAGCAAAAGAAAACAAAAAGGTTTGTACAGTTGGGTTGAAGCAACTTTCATCAGTCAATCTATTCATCAATCCGTATTGATTTGAAAGGGACAACTGTTATTATTGCTCCATTCTCTATTAATTTGGATTATCGGTATCTTGAATGACAAATTTTTTAAGGATGAGTTTAGCAACCTTAGCTGATTGAAATTTCAAATTTTCCTGTCCTAGTTGACTTTGTAGACGGAAAACATTTATGCTATCCTTGATCTTTCTGAGTATACTGTATAGAGATAGGGAGCCAGTTTTACTTATAAACACCTAACCTGATGAGGCACCTCTCTTTTGTACATCTGATGTAGGATCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGGTATAAAACCTCTGAGTGGAAGCATGCAAATTTCTTGTCAAATCCCATAGTCTTTAGCAACTTATCCTAGATCGCGAAACTGCTTTTCTTTATCGTAAACTGAAGATTGAATTGACTGAAAATTGGGTGTTAACGTAAGATCTTTCTTATGACAAGTATGGATGTTAACAGGAGGATCTAGGCTTATGCAGTTGAACTCTGAATTTGGGTTCTGAAGTTCTGTCTATTCTCATGACTGCGCTAATTATAGTGAACTGTAATATGGAAAAAATAAATAGATAATGAAACGTTTTGTATCAGATATGTTCTAGTTCTCATTCTTTTCTATGTGATTGTATAATTCTATGCGTAGGATTTTATTCCAAATTCCATATAAATTAGTGTACTTAGGAAGACATGATCCCCCATCATTACTATGAAATGCATAATCATCAATAAGTCTGAGGAATAGTTTGCTGGGAAGAAACAGAGTTTGGACTAAGCCCTTCTCTTTTTTTCTTTTTAGTGAGAAAAGAAAACTGTGAATATGAAACTCTATTTTTTTAAGATGTGAAGCATTTGACACATGCATTAATAGATTGTTTAGGTATTGATATTACTGGTAAAGTGAAACTTGATTGTGCTTGATTTCAGAAAATGGAGAAGACATTCAAGTATTGAGATATGAATATGGGCAGAAGTACGATGCTCACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTGGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGTCCGTCCTTGTATTCCCCTTCAATCCAATTAATTGCAACTTATTTTTCTTTTTTTGCCTAAACTCTGACTATAAACAAGTACCTTGGTTTTCTTAGTTCAATATTCTTTGCAGGAAGATACATCTTTCCTACTTATTCACTTACTTTATCTAACGCTTTTGGCTCTGAAAACTAATTAGAACTGGAAATTTTTCTCGTCTTGGCATTTATATGTGCTGTCATTAATTGATGACTTTTTATTCAATATTTGATGTCTTTGTTCATTCATGTGAGTTCAGGAATCTCAAAGACGTCAGGCTTCCGAAACAAACCAAGATCTCTCAGATTGTGCAAAGAAAGGCATAGCAGGTGAGTGTTTTAAGTAGTAGTAGAAATCAATTTTCTAACTTCTGTCCTGTTCGTAACATTCATCCAAACTTGTTGAGATTTCTCTCTTTAACCTCCATGTTCAAAATATTCAGTAAAACCTCGGAAAGGCGATGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTTGATTCCTTCGACACGATCGCGAGAGACCATACAAATTGTGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCAGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGCAAGGCATGCTCATAAACTTGGTCCATTCTTTATAATGAGCATTCCCTTGCCTTTTCGTTTTTGTACCAAAAACAGAAATGTTAGTTTTTCGAGAGCATTTTCATTGAAATGTTTTGTGAGAGTTAGCATTTGTATTGATTACTCAATCATGTAACATTATTTGAACACTAACGTAGTTTGCAAAGTTATTTTGGTTTGTATGGATCGTCCAATTATAACTCAGTTAAAGACCTATTTGGATTGATAAGTGCTTAAATATACATTTAG

mRNA sequence

GTGAGACTTTAGAAAATTTGACAGTTAACAGCTTGAAGAAGAGATGGAAATCTGAAAATTTTGTGAAGAAAAAGAAAAATTTTATTGCATTTTTCTTCTTCTTCTTCATTTCTTCTCTTTCTCTTTTGGTCCGATTCAGTTCCATGGCTGAATTTCTCCGTTTCAATCTACTTTTTCTTTTCACATTAACCATTTCCTACCTTCTCCGGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCCTGGTCTCCTCGGGCTTTTGTGTATGAAGGTTTTCTCACGGATTTAGAATGCGATCATCTCATTTCCCTTGCTAAAGCGGAGCTGAAGAGATCCTCTGTTGCGGATAATTTGTCCGGAGAGAGCAAAGTCAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGATCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGAGAAGACATTCAAGTATTGAGATATGAATATGGGCAGAAGTACGATGCTCACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTGGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGTCAGGCTTCCGAAACAAACCAAGATCTCTCAGATTGTGCAAAGAAAGGCATAGCAGTAAAACCTCGGAAAGGCGATGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTTGATTCCTTCGACACGATCGCGAGAGACCATACAAATTGTGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCAGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGCAAGGCATGCTCATAAACTTGGTCCATTCTTTATAATGAGCATTCCCTTGCCTTTTCGTTTTTGTACCAAAAACAGAAATGTTAGTTTTTCGAGAGCATTTTCATTGAAATGTTTTGTGAGAGTTAGCATTTGTATTGATTACTCAATCATGTAACATTATTTGAACACTAACGTAGTTTGCAAAGTTATTTTGGTTTGTATGGATCGTCCAATTATAACTCAGTTAAAGACCTATTTGGATTGATAAGTGCTTAAATATACATTTAG

Coding sequence (CDS)

ATGGCTGAATTTCTCCGTTTCAATCTACTTTTTCTTTTCACATTAACCATTTCCTACCTTCTCCGGCGAGCTTCAGCCTCCTATGCAGGTTCCGCTAGCTCAATCGTCAACCCTGCAAAAGTCAAACAGATTTCCTGGTCTCCTCGGGCTTTTGTGTATGAAGGTTTTCTCACGGATTTAGAATGCGATCATCTCATTTCCCTTGCTAAAGCGGAGCTGAAGAGATCCTCTGTTGCGGATAATTTGTCCGGAGAGAGCAAAGTCAGCGAGGTCCGAACTAGCTCTGGGGCGTTTATTCATAAAGCCAAGGATCCTATTGTTTCTGGTATAGAAGACAAAATTGCAGCATGGACATTTCTGCCAAAAGAAAATGGAGAAGACATTCAAGTATTGAGATATGAATATGGGCAGAAGTACGATGCTCACTTTGATTACTTTGCTGACAAGGTTAACATTGCCCGAGGTGGACATCGAATGGCAACCGTTCTCATGTATCTTTCCGACGTGGAAAAAGGCGGTGAAACTGTGTTTCCTTCTGCAGAGGAATCTCAAAGACGTCAGGCTTCCGAAACAAACCAAGATCTCTCAGATTGTGCAAAGAAAGGCATAGCAGTAAAACCTCGGAAAGGCGATGCTCTTCTCTTCTTCAGCCTCCATCCAAATGCTATTCCAGACACAAGTAGTCTACATGGTGGGTGCCCTGTGATTGAAGGTGAGAAATGGTCAGCAACAAAGTGGATTCATGTTGATTCCTTCGACACGATCGCGAGAGACCATACAAATTGTGGTGATGAAAATCCAAGTTGTGAGAGATGGGCTGAACTCGGCGAGTGCACGAATAACCCAGAGTATATGGTGGGATCTCCTGAGCTTCCTGGCTACTGCAGGAAAAGTTGCAAGGCATGCTCATAA

Protein sequence

MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKACS
Homology
[Close]
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 2.6e-129
Identity = 228/297 (76.77%), Postives = 247/297 (83.16%), Query Frame = 0 

Query: 6   RFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHL 65
           R  LL  F    S LL ++S S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH+
Sbjct: 3   RRGLLISFFAIFSVLL-QSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHM 62

Query: 66  ISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENG 125
           +SLAKA LKRS+VADN SGESK SEVRTSSG FI K KDPIVSGIEDKI+ WTFLPKENG
Sbjct: 63  VSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENG 122

Query: 126 EDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQR 185
           EDIQVLRYE+GQKYDAHFDYF DKVNI RGGHRMAT+LMYLS+V KGGETVFP AE   R
Sbjct: 123 EDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSR 182

Query: 186 RQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATK 245
           R  SE  +DLSDCAK+GIAVKPRKGDALLFF+LHP+AIPD  SLHGGCPVIEGEKWSATK
Sbjct: 183 RVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATK 242

Query: 246 WIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           WIHVDSFD I     NC D N SCERWA LGECT NPEYMVG+ ELPGYCR+SCKAC
Sbjct: 243 WIHVDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 6.6e-125
Identity = 216/291 (74.23%), Postives = 243/291 (83.51%), Query Frame = 0 

Query: 12  LFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKA 71
           L  + I  +L ++S     S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDHLISLAK 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 72  ELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVL 131
            L+RS+VADN +GES+VS+VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 132 RYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASET 191
           RYE+GQKYDAHFDYF DKVNIARGGHR+ATVL+YLS+V KGGETVFP A+E  RR  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 192 NQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDS 251
             DLSDCAKKGIAVKP+KG+ALLFF+L  +AIPD  SLHGGCPVIEGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 252 FDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           FD I     NC D N SCERWA LGEC  NPEYMVG+PE+PG CR+SCKAC
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 4.6e-102
Identity = 189/314 (60.19%), Postives = 232/314 (73.89%), Query Frame = 0 

Query: 4   FLRFNLLFLFTLTI-----SYLLRRASASYAG-------SASSI-VNPAKVKQISWSPRA 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGI 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES  SEVRTSSG F+ K +D IVS +
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNV 125

Query: 124 EDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVE 183
           E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATVLMYLS+VE
Sbjct: 126 EAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVE 185

Query: 184 KGGETVFPSAEESQRRQASETNQD-LSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSL 243
           KGGETVFP      + +A++   D  ++CAK+G AVKPRKGDALLFF+LHPNA  D++SL
Sbjct: 186 KGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSL 245

Query: 244 HGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSP 303
           HG CPV+EGEKWSAT+WIHV SF+      + C DEN SCE+WA+ GEC  NP YMVGS 
Sbjct: 246 HGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSD 305

[Close]
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 3.7e-91
Identity = 167/277 (60.29%), Postives = 205/277 (74.01%), Query Frame = 0 

Query: 27  SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKAELKRSS-VADNLSGE 86
           S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDHLI LAK +L++S  VAD  SGE
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 87  SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDY 146
           S+ SEVRTSSG F+ K +D IV+ +E K+AAWTFLP+ENGE +Q+L YE GQKYD HFDY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 147 FADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAV 206
           F DK  +  GGHR+ATVLMYLS+V KGGETVFP+ +    +   ++    S CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDS---WSKCAKQGYAV 197

Query: 207 KPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDE 266
           KPRKGDALLFF+LH N   D +SLHG CPVIEGEKWSAT+WIHV SF    +    C D+
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSF---GKKKLVCVDD 257

Query: 267 NPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           + SC+ WA+ GEC  NP YMVGS    G+CRKSCKAC
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match: Q9LN20 (Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-66
Identity = 118/209 (56.46%), Postives = 157/209 (75.12%), Query Frame = 0 

Query: 44  ISWSPRAFVYEGFLTDLECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAK 103
           +SW PRAFVY  FL+  EC++LISLAK  + +S+V D+ +G+SK S VRTSSG F+ + +
Sbjct: 79  LSWEPRAFVYHNFLSKEECEYLISLAKPHMVKSTVVDSETGKSKDSRVRTSSGTFLRRGR 138

Query: 104 DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVL 163
           D I+  IE +IA +TF+P ++GE +QVL YE GQKY+ H+DYF D+ N   GG RMAT+L
Sbjct: 139 DKIIKTIEKRIADYTFIPADHGEGLQVLHYEAGQKYEPHYDYFVDEFNTKNGGQRMATML 198

Query: 164 MYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAI 223
           MYLSDVE+GGETVFP+A  +    +     +LS+C KKG++VKPR GDALLF+S+ P+A 
Sbjct: 199 MYLSDVEEGGETVFPAA--NMNFSSVPWYNELSECGKKGLSVKPRMGDALLFWSMRPDAT 258

Query: 224 PDTSSLHGGCPVIEGEKWSATKWIHVDSF 253
            D +SLHGGCPVI G KWS+TKW+HV  +
Sbjct: 259 LDPTSLHGGCPVIRGNKWSSTKWMHVGEY 285

[Close]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match: A0A1S3AWU7 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 1.4e-173
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 ACS 304
           ACS
Sbjct: 301 ACS 303

[Close]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match: A0A5A7U593 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G00280 PE=3 SV=1)

HSP 1 Score: 606.7 bits (1563), Expect = 5.4e-170
Identity = 300/303 (99.01%), Postives = 300/303 (99.01%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISW   AFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISW---AFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 ACS 304
           ACS
Sbjct: 301 ACS 300

[Close]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match: A0A0A0L5Q6 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.3e-167
Identity = 291/303 (96.04%), Postives = 298/303 (98.35%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAE L FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 96

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSG+SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 97  ECDHLISLAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 156

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PK+NGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 157 PKDNGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 216

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 217 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 276

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWI VDSFD + RDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 277 WSATKWIRVDSFDMVVRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 336

Query: 301 ACS 304
           ACS
Sbjct: 337 ACS 339

[Close]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match: A0A6J1C7M6 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125 PE=3 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 7.8e-161
Identity = 278/302 (92.05%), Postives = 291/302 (96.36%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA F   +LLF F+L+IS LLRRAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRS+VADNLSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLS+VEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPR+GDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTCSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI RDHTNC DE+ SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 AC 303
            C
Sbjct: 301 VC 302

[Close]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match: A0A6J1I971 (Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 4.3e-159
Identity = 276/302 (91.39%), Postives = 289/302 (95.70%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+F   NLLF+ +++IS LLRRAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAKFCSCNLLFILSISISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRS+VAD+LSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSAVADHLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF DKVNIARGGHRMATVLMYLS+VEKGGETVFP+A
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFTDKVNIARGGHRMATVLMYLSNVEKGGETVFPNA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPRKGDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAVPDTKSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI  DHT+C D N SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIVSDHTSCVDNNASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 AC 303
            C
Sbjct: 301 VC 302

[Close]
BLAST of IVF0010169 vs. NCBI nr
Match: XP_008438765.1 (PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo])

HSP 1 Score: 615 bits (1585), Expect = 4.40e-222
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 ACS 303
           ACS
Sbjct: 301 ACS 303

[Close]
BLAST of IVF0010169 vs. NCBI nr
Match: KAA0049426.1 (putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa] >TYK16104.1 putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 603 bits (1554), Expect = 2.09e-217
Identity = 300/303 (99.01%), Postives = 300/303 (99.01%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISW   AFVYEGFLTDL
Sbjct: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISW---AFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 ACS 303
           ACS
Sbjct: 301 ACS 300

[Close]
BLAST of IVF0010169 vs. NCBI nr
Match: XP_004134175.2 (probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN57053.1 hypothetical protein Csa_010114 [Cucumis sativus])

HSP 1 Score: 593 bits (1530), Expect = 4.06e-213
Identity = 291/303 (96.04%), Postives = 298/303 (98.35%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MAE L FNLLFLFTL+IS+LL+RASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 37  MAELLCFNLLFLFTLSISFLLQRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 96

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSG+SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 97  ECDHLISLAKAELKRSSVADNLSGKSKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 156

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PK+NGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 157 PKDNGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 216

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK
Sbjct: 217 EESQRRQASETNEDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 276

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWI VDSFD + RDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 277 WSATKWIRVDSFDMVVRDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 336

Query: 301 ACS 303
           ACS
Sbjct: 337 ACS 339

[Close]
BLAST of IVF0010169 vs. NCBI nr
Match: XP_038903083.1 (probable prolyl 4-hydroxylase 4 [Benincasa hispida])

HSP 1 Score: 582 bits (1499), Expect = 5.67e-209
Identity = 286/303 (94.39%), Postives = 293/303 (96.70%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA+F  FNLLF F+L+IS LLRRAS+SYAGSASSIVNPAKVKQISW+PRAFVYEGFLTDL
Sbjct: 1   MAKFYCFNLLFFFSLSISCLLRRASSSYAGSASSIVNPAKVKQISWNPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL
Sbjct: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN DLSDCAKKGIAVKPRKGDALLFFSLHPNAIPD SSLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNDDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDISSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFD I RDHT+C DENPSCERWAEL ECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDKIIRDHTDCADENPSCERWAELSECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 ACS 303
           AC+
Sbjct: 301 ACA 303

[Close]
BLAST of IVF0010169 vs. NCBI nr
Match: XP_022137761.1 (probable prolyl 4-hydroxylase 4 [Momordica charantia])

HSP 1 Score: 572 bits (1475), Expect = 2.49e-205
Identity = 278/302 (92.05%), Postives = 291/302 (96.36%), Query Frame = 0 

Query: 1   MAEFLRFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60
           MA F   +LLF F+L+IS LLRRAS+SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL
Sbjct: 1   MARFCSCSLLFFFSLSISLLLRRASSSYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDL 60

Query: 61  ECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFL 120
           ECDH+ISLAKAELKRS+VADNLSGESKVSEVRTSSGAFIHKAKDPI+SGIEDKIAAWTFL
Sbjct: 61  ECDHIISLAKAELKRSAVADNLSGESKVSEVRTSSGAFIHKAKDPIISGIEDKIAAWTFL 120

Query: 121 PKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSA 180
           PKENGEDIQVLRYEYGQKYDAHFDYF+DKVNIARGGHRMATVLMYLS+VEKGGETVFPSA
Sbjct: 121 PKENGEDIQVLRYEYGQKYDAHFDYFSDKVNIARGGHRMATVLMYLSNVEKGGETVFPSA 180

Query: 181 EESQRRQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEK 240
           EESQRRQASETN+DLSDCAKKGIAVKPR+GDALLFFSLHPNA+PDT SLHGGCPVIEGEK
Sbjct: 181 EESQRRQASETNEDLSDCAKKGIAVKPRRGDALLFFSLHPNAVPDTCSLHGGCPVIEGEK 240

Query: 241 WSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300
           WSATKWIHVDSFDTI RDHTNC DE+ SCERWAELGECTNNPEYMVGSPELPGYCRKSCK
Sbjct: 241 WSATKWIHVDSFDTILRDHTNCADEHASCERWAELGECTNNPEYMVGSPELPGYCRKSCK 300

Query: 301 AC 302
            C
Sbjct: 301 VC 302

[Close]
BLAST of IVF0010169 vs. TAIR 10
Match: AT5G18900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 463.0 bits (1190), Expect = 1.8e-130
Identity = 228/297 (76.77%), Postives = 247/297 (83.16%), Query Frame = 0 

Query: 6   RFNLLFLFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHL 65
           R  LL  F    S LL ++S S   S+S  VNP+KVKQ+S  PRAFVYEGFLT+LECDH+
Sbjct: 3   RRGLLISFFAIFSVLL-QSSTSLISSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHM 62

Query: 66  ISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENG 125
           +SLAKA LKRS+VADN SGESK SEVRTSSG FI K KDPIVSGIEDKI+ WTFLPKENG
Sbjct: 63  VSLAKASLKRSAVADNDSGESKFSEVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENG 122

Query: 126 EDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQR 185
           EDIQVLRYE+GQKYDAHFDYF DKVNI RGGHRMAT+LMYLS+V KGGETVFP AE   R
Sbjct: 123 EDIQVLRYEHGQKYDAHFDYFHDKVNIVRGGHRMATILMYLSNVTKGGETVFPDAEIPSR 182

Query: 186 RQASETNQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATK 245
           R  SE  +DLSDCAK+GIAVKPRKGDALLFF+LHP+AIPD  SLHGGCPVIEGEKWSATK
Sbjct: 183 RVLSENKEDLSDCAKRGIAVKPRKGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATK 242

Query: 246 WIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           WIHVDSFD I     NC D N SCERWA LGECT NPEYMVG+ ELPGYCR+SCKAC
Sbjct: 243 WIHVDSFDRIVTPSGNCTDMNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]
BLAST of IVF0010169 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 448.4 bits (1152), Expect = 4.7e-126
Identity = 216/291 (74.23%), Postives = 243/291 (83.51%), Query Frame = 0 

Query: 12  LFTLTISYLLRRASASYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKA 71
           L  + I  +L ++S     S SSI+NP+KVKQ+S  PRAFVYEGFLTDLECDHLISLAK 
Sbjct: 9   LLFVAILLVLLQSSTCLISSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKE 68

Query: 72  ELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVL 131
            L+RS+VADN +GES+VS+VRTSSG FI K KDPIVSGIEDK++ WTFLPKENGED+QVL
Sbjct: 69  NLQRSAVADNDNGESQVSDVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVL 128

Query: 132 RYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASET 191
           RYE+GQKYDAHFDYF DKVNIARGGHR+ATVL+YLS+V KGGETVFP A+E  RR  SE 
Sbjct: 129 RYEHGQKYDAHFDYFHDKVNIARGGHRIATVLLYLSNVTKGGETVFPDAQEFSRRSLSEN 188

Query: 192 NQDLSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDS 251
             DLSDCAKKGIAVKP+KG+ALLFF+L  +AIPD  SLHGGCPVIEGEKWSATKWIHVDS
Sbjct: 189 KDDLSDCAKKGIAVKPKKGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHVDS 248

Query: 252 FDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           FD I     NC D N SCERWA LGEC  NPEYMVG+PE+PG CR+SCKAC
Sbjct: 249 FDKILTHDGNCTDVNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]
BLAST of IVF0010169 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 372.5 bits (955), Expect = 3.3e-103
Identity = 189/314 (60.19%), Postives = 232/314 (73.89%), Query Frame = 0 

Query: 4   FLRFNLLFLFTLTI-----SYLLRRASASYAG-------SASSI-VNPAKVKQISWSPRA 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHLISLAKAELKRSSVADNLSGESKVSEVRTSSGAFIHKAKDPIVSGI 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES  SEVRTSSG F+ K +D IVS +
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKRQDDIVSNV 125

Query: 124 EDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATVLMYLSDVE 183
           E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATVLMYLS+VE
Sbjct: 126 EAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATVLMYLSNVE 185

Query: 184 KGGETVFPSAEESQRRQASETNQD-LSDCAKKGIAVKPRKGDALLFFSLHPNAIPDTSSL 243
           KGGETVFP      + +A++   D  ++CAK+G AVKPRKGDALLFF+LHPNA  D++SL
Sbjct: 186 KGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTDSNSL 245

Query: 244 HGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNNPEYMVGSP 303
           HG CPV+EGEKWSAT+WIHV SF+      + C DEN SCE+WA+ GEC  NP YMVGS 
Sbjct: 246 HGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKNPTYMVGSD 305

[Close]
BLAST of IVF0010169 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 350.5 bits (898), Expect = 1.3e-96
Identity = 185/322 (57.45%), Postives = 227/322 (70.50%), Query Frame = 0 

Query: 4   FLRFNLLFLFTLTI-----SYLLRRASASYAG-------SASSI-VNPAKVKQISWSPRA 63
           FL F+L FLFTL +     +  L R+S +  G       SASS   +P +V Q+SW+PR 
Sbjct: 6   FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIKMKTSASSFGFDPTRVTQLSWTPRV 65

Query: 64  FVYEGFLTDLECDHLISLAKAELKRSSVADNLSGES-----KVSEVRTSSGAFIHKAK-- 123
           F+YEGFL+D ECDH I LAK +L++S VADN SGES      VS VR SS    +     
Sbjct: 66  FLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSVVRQSSSFIANMDSLE 125

Query: 124 -DPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDYFADKVNIARGGHRMATV 183
            D IVS +E K+AAWTFLP+ENGE +Q+L YE GQKY+ HFDYF D+ N+  GGHR+ATV
Sbjct: 126 IDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATV 185

Query: 184 LMYLSDVEKGGETVFPSAEESQRRQASETNQD-LSDCAKKGIAVKPRKGDALLFFSLHPN 243
           LMYLS+VEKGGETVFP      + +A++   D  ++CAK+G AVKPRKGDALLFF+LHPN
Sbjct: 186 LMYLSNVEKGGETVFP----MWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPN 245

Query: 244 AIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDENPSCERWAELGECTNN 303
           A  D++SLHG CPV+EGEKWSAT+WIHV SF+      + C DEN SCE+WA+ GEC  N
Sbjct: 246 ATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQSGCMDENVSCEKWAKAGECQKN 305

[Close]
BLAST of IVF0010169 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 336.3 bits (861), Expect = 2.6e-92
Identity = 167/277 (60.29%), Postives = 205/277 (74.01%), Query Frame = 0 

Query: 27  SYAGSASSIVNPAKVKQISWSPRAFVYEGFLTDLECDHLISLAKAELKRSS-VADNLSGE 86
           S   S S  V+P ++ Q+SW+PRAF+Y+GFL+D ECDHLI LAK +L++S  VAD  SGE
Sbjct: 18  SQISSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGE 77

Query: 87  SKVSEVRTSSGAFIHKAKDPIVSGIEDKIAAWTFLPKENGEDIQVLRYEYGQKYDAHFDY 146
           S+ SEVRTSSG F+ K +D IV+ +E K+AAWTFLP+ENGE +Q+L YE GQKYD HFDY
Sbjct: 78  SEDSEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDY 137

Query: 147 FADKVNIARGGHRMATVLMYLSDVEKGGETVFPSAEESQRRQASETNQDLSDCAKKGIAV 206
           F DK  +  GGHR+ATVLMYLS+V KGGETVFP+ +    +   ++    S CAK+G AV
Sbjct: 138 FYDKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDS---WSKCAKQGYAV 197

Query: 207 KPRKGDALLFFSLHPNAIPDTSSLHGGCPVIEGEKWSATKWIHVDSFDTIARDHTNCGDE 266
           KPRKGDALLFF+LH N   D +SLHG CPVIEGEKWSAT+WIHV SF    +    C D+
Sbjct: 198 KPRKGDALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVRSF---GKKKLVCVDD 257

Query: 267 NPSCERWAELGECTNNPEYMVGSPELPGYCRKSCKAC 303
           + SC+ WA+ GEC  NP YMVGS    G+CRKSCKAC
Sbjct: 258 HESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

The following BLAST results are available for this feature:
BLAST of IVF0010169 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8LAN32.6e-12976.77Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
F4JAU36.6e-12574.23Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q8L9704.6e-10260.19Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4J0A83.7e-9160.29Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q9LN202.4e-6656.46Probable prolyl 4-hydroxylase 3 OS=Arabidopsis thaliana OX=3702 GN=P4H3 PE=2 SV=... [more]
BLAST of IVF0010169 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3AWU71.4e-173100.00Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103483779 PE=3 S... [more]
A0A5A7U5935.4e-17099.01Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0L5Q63.3e-16796.04Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_3G150820 PE=... [more]
A0A6J1C7M67.8e-16192.05Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111009125... [more]
A0A6J1I9714.3e-15991.39Procollagen-proline 4-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111472232 PE... [more]
BLAST of IVF0010169 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008438765.14.40e-222100.00PREDICTED: probable prolyl 4-hydroxylase 4 [Cucumis melo][more]
KAA0049426.12.09e-21799.01putative prolyl 4-hydroxylase 4 [Cucumis melo var. makuwa] >TYK16104.1 putative ... [more]
XP_004134175.24.06e-21396.04probable prolyl 4-hydroxylase 4 [Cucumis sativus] >KGN57053.1 hypothetical prote... [more]
XP_038903083.15.67e-20994.39probable prolyl 4-hydroxylase 4 [Benincasa hispida][more]
XP_022137761.12.49e-20592.05probable prolyl 4-hydroxylase 4 [Momordica charantia][more]
BLAST of IVF0010169 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G18900.11.8e-13076.772-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G06300.14.7e-12674.23P4H isoform 2 [more]
AT3G28480.13.3e-10360.19Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.21.3e-9657.45Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.12.6e-9260.29Oxoglutarate/iron-dependent oxygenase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 48..248
e-value: 2.2E-61
score: 219.9
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 261..303
e-value: 3.4E-7
score: 39.9
IPR003582ShKT domainPFAMPF01549ShKcoord: 261..302
e-value: 7.8E-4
score: 19.9
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 262..302
score: 8.658319
IPR044862Prolyl 4-hydroxylase alpha subunit, Fe(2+) 2OG dioxygenase domainPFAMPF136402OG-FeII_Oxy_3coord: 128..248
e-value: 3.0E-21
score: 76.1
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 40..249
e-value: 5.4E-77
score: 260.2
NoneNo IPR availablePANTHERPTHR10869:SF175PROLYL 4-HYDROXYLASE SUBUNIT ALPHA-LIKE PROTEINcoord: 10..302
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 10..302
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 124..249
score: 12.667426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0010169.1IVF0010169.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018401 peptidyl-proline hydroxylation to 4-hydroxy-L-proline
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen