IVF0009976 (gene) Melon (IVF77) v1

Overview
NameIVF0009976
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionMembrane-associated kinase regulator 4
Locationchr10: 5433764 .. 5434810 (+)
RNA-Seq ExpressionIVF0009976
SyntenyIVF0009976
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGTTGACATTCCATCTTGTGATCCATCAGATGATGACTATATAGACATTGAAATTGGTTCATACACTAACTTTCGCTGCTGCTCCAAAAGCTCGCCGCGACAATCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGAGGGAGAGAGCCAACGACTTCTCCAGCTGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGATAGAGAAACTCTTGCAAAAGTCTAGTTCTGATTATGATTACAGAAAAGATACGTTTGAAGAATTTTACACCACTCCATTAACCACTTCACTCAATACACCTACCAGTACTCCTTTTGACTCCTGCAACATTTCTCCTTCTGAATCTTTTCGGATTAGTAGGGAGCTAAATCCGGACGAATATCTTCTAGAGTATTCAGCAGATGTGAGCTATTTGAATGGTGAAAGATCGAAAAAGTCCTGGACCAAAAAGCTTAAGCATTCTACCCTTTGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTTTTTACCAAATCAGGTTGCTCAGATGAGTCCTGTGCAGCAGCTGCAAAGATTGCAGATGAAGGATCAGCCTCAAAACCCAATGAATGTTCAAAAAAGGGAGCTAGAGTGGAAAAGAGAAAGCCATTTGGACCAATTTCGAGCTCTATCGCCAATTCCAACAAAGACAAAGGCAATACAGAAAACAGTAGTCGCAACAGAAGATCATTCTCATTGGTTATTAAGAGACAACCAATTATCAAATCATCGTCTTTTGCACCATTACATCCCACTTCATCTTCATGTCCAAATATTTCAAGCGGGAAATACCTAAAGAGATGTGGGAGTGTGAATTCAGAGATTGAGAACTCGATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCAATCTCAGCAACCATTGCGTTCAAGAAAGACAGTTAGTGAAGTTGGGATTTACTTATTATCATCTTCAACCCTTACAGTTTCTGATGATCAGGAAATACCAGAACTTGGCAGGGGATGA

mRNA sequence

ATGGCTGTTGACATTCCATCTTGTGATCCATCAGATGATGACTATATAGACATTGAAATTGGTTCATACACTAACTTTCGCTGCTGCTCCAAAAGCTCGCCGCGACAATCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGAGGGAGAGAGCCAACGACTTCTCCAGCTGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGATAGAGAAACTCTTGCAAAAGTCTAGTTCTGATTATGATTACAGAAAAGATACGTTTGAAGAATTTTACACCACTCCATTAACCACTTCACTCAATACACCTACCAGTACTCCTTTTGACTCCTGCAACATTTCTCCTTCTGAATCTTTTCGGATTAGTAGGGAGCTAAATCCGGACGAATATCTTCTAGAGTATTCAGCAGATGTGAGCTATTTGAATGGTGAAAGATCGAAAAAGTCCTGGACCAAAAAGCTTAAGCATTCTACCCTTTGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTTTTTACCAAATCAGGTTGCTCAGATGAGTCCTGTGCAGCAGCTGCAAAGATTGCAGATGAAGGATCAGCCTCAAAACCCAATGAATGTTCAAAAAAGGGAGCTAGAGTGGAAAAGAGAAAGCCATTTGGACCAATTTCGAGCTCTATCGCCAATTCCAACAAAGACAAAGGCAATACAGAAAACAGTAGTCGCAACAGAAGATCATTCTCATTGGTTATTAAGAGACAACCAATTATCAAATCATCGTCTTTTGCACCATTACATCCCACTTCATCTTCATGTCCAAATATTTCAAGCGGGAAATACCTAAAGAGATGTGGGAGTGTGAATTCAGAGATTGAGAACTCGATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCAATCTCAGCAACCATTGCGTTCAAGAAAGACAGTTAGTGAAGTTGGGATTTACTTATTATCATCTTCAACCCTTACAGTTTCTGATGATCAGGAAATACCAGAACTTGGCAGGGGATGA

Coding sequence (CDS)

ATGGCTGTTGACATTCCATCTTGTGATCCATCAGATGATGACTATATAGACATTGAAATTGGTTCATACACTAACTTTCGCTGCTGCTCCAAAAGCTCGCCGCGACAATCCAGAGAGTTCGAGTTTCAAATGTCTTTGAATTCTCGAGGGAGAGAGCCAACGACTTCTCCAGCTGATGAGCTCTTCTACAATGGGAAGCTTCTTCCCCTTCATCATCCCCCACGCCTGCAAATGATAGAGAAACTCTTGCAAAAGTCTAGTTCTGATTATGATTACAGAAAAGATACGTTTGAAGAATTTTACACCACTCCATTAACCACTTCACTCAATACACCTACCAGTACTCCTTTTGACTCCTGCAACATTTCTCCTTCTGAATCTTTTCGGATTAGTAGGGAGCTAAATCCGGACGAATATCTTCTAGAGTATTCAGCAGATGTGAGCTATTTGAATGGTGAAAGATCGAAAAAGTCCTGGACCAAAAAGCTTAAGCATTCTACCCTTTGTCTCAAGCTGAAAGCTTCCAGGGCTTACATCAAATCTTTTTTTACCAAATCAGGTTGCTCAGATGAGTCCTGTGCAGCAGCTGCAAAGATTGCAGATGAAGGATCAGCCTCAAAACCCAATGAATGTTCAAAAAAGGGAGCTAGAGTGGAAAAGAGAAAGCCATTTGGACCAATTTCGAGCTCTATCGCCAATTCCAACAAAGACAAAGGCAATACAGAAAACAGTAGTCGCAACAGAAGATCATTCTCATTGGTTATTAAGAGACAACCAATTATCAAATCATCGTCTTTTGCACCATTACATCCCACTTCATCTTCATGTCCAAATATTTCAAGCGGGAAATACCTAAAGAGATGTGGGAGTGTGAATTCAGAGATTGAGAACTCGATTCAGGGAGCAATTGCACACTGCAAGCAGTCTCAATCTCAGCAACCATTGCGTTCAAGAAAGACAGTTAGTGAAGTTGGGATTTACTTATTATCATCTTCAACCCTTACAGTTTCTGATGATCAGGAAATACCAGAACTTGGCAGGGGATGA

Protein sequence

MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Homology
BLAST of IVF0009976 vs. ExPASy Swiss-Prot
Match: O80624 (Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 GN=MAKR4 PE=3 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 8.3e-63
Identity = 169/360 (46.94%), Postives = 225/360 (62.50%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNF--RCCSKSSPRQSREFEFQMS-LNSRGREPTTSP 60
           MA  +  CD  ++DYID+E+ S+TN   +  S + P   REFEFQMS L     + TTSP
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTNLVRKTLSNNYP---REFEFQMSHLCPLEIDKTTSP 60

Query: 61  ADELFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTT-SLNTP--TS 120
           ADELFY GKLLPLH PPRLQM++K+L+      DY  D  +EFY+TPL T ++ TP  ++
Sbjct: 61  ADELFYKGKLLPLHLPPRLQMVQKILE------DYTFD--DEFYSTPLATGTVTTPVTSN 120

Query: 121 TPFDSCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKL---KHSTLCLK 180
           TPF+SC +SP+ES ++S+ELNP++Y LEYS  +     +  KKSWT KL   K S+L  K
Sbjct: 121 TPFESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTK 180

Query: 181 LKASRAYIKSFFTKSGCSDE-SCA-AAAKIADEGSASKPNECSKKGARVEKRKPFGPISS 240
           +KASRAY++SFF K+ CSDE SCA +AA++ADE S            R  + KPFG I +
Sbjct: 181 IKASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIKT 240

Query: 241 SIANSNKDKGNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCG 300
                 K + N   S  +RRSFS+ ++RQ    S++       SS+       ++LKR  
Sbjct: 241 ---ERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNN------KSSNSLGFRPLQFLKRST 300

Query: 301 SVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS+S +   DDQE  ++ RG
Sbjct: 301 SSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFRG 328

BLAST of IVF0009976 vs. ExPASy Swiss-Prot
Match: Q9ZUS8 (Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 GN=MAKR3 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 6.0e-29
Identity = 131/350 (37.43%), Postives = 176/350 (50.29%), Query Frame = 0

Query: 12  DDDYIDIEI----------GSYTNFRCCSKSSPRQSREFEFQMSLNS-RGREPTTSPADE 71
           DD YID+E+           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTP--FD 131
           LFY G+LLPLH PPRL+M++KLL  SSS     +        TP++        +P  F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATE--------TPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSES--FRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASR 191
           SC I   E+  F IS EL   +  +E        N      SW+KK+KHS++  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNK 251
           AYIK+ F+K  CSD S        +     +P++ S+      K+ PF            
Sbjct: 198 AYIKALFSKQACSDSS------EINPRFKIEPSKVSR------KKNPF------------ 257

Query: 252 DKGNTENSSR-NRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEI 311
              N+EN    +RRSFS VI+R    K S+ +    +SSS  ++SS       GS++ + 
Sbjct: 258 --VNSENPLLIHRRSFSGVIQRHSQAKCSTSS---SSSSSASSLSSSFSFGSNGSLDLQT 313

Query: 312 --------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVS 338
                   +NSI+GAI HCKQS + +  +S  T SE     L SS  +VS
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESE-----LCSSRTSVS 313

BLAST of IVF0009976 vs. ExPASy Swiss-Prot
Match: Q3E936 (Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 GN=MAKR1 PE=1 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.3e-04
Identity = 94/335 (28.06%), Postives = 134/335 (40.00%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMIEKL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL ++  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDY----------DYRKDTFEEFYTTPLTTSLNTPTSTPFDSC----------------- 149
           SDY             +D+ E   +T  T S       P D C                 
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSRPSSVTDDEDFFFK 134

Query: 150 ---NISPSESFRISR-----ELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKL 209
              N S S  F +SR     + +P   L  +S+  S      +  S   K   ST    +
Sbjct: 135 PPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSSTATTAAAPSS--VKRMSSTAKEVI 194

Query: 210 KASRAYIKSFFTKSGCSDESCAAAAKIADEGSAS-KPNECSKKGARVEKRKPFGP--ISS 269
           +     +K  + K      S   ++ I  E S+S K +  + +G          P  +SS
Sbjct: 195 RKYMKKVKPLYEKL-----SPKQSSNIKTESSSSLKDSGNNIRGTTTVTTVTAAPTVVSS 254

Query: 270 SIANSNKDKGNTENSSRNRR---SFSLVIKRQP----IIKSSSFAPLHPTSSSCPNISSG 318
               S    GN    ++  R   S    ++  P    ++    F P+H    SC + SS 
Sbjct: 255 GGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGGF-PVH--QGSCSSSSSN 314

BLAST of IVF0009976 vs. ExPASy TrEMBL
Match: A0A5A7V1W4 (Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold321G00070 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.5e-192
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Sbjct: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of IVF0009976 vs. ExPASy TrEMBL
Match: A0A1S3CEW1 (probable membrane-associated kinase regulator 4 OS=Cucumis melo OX=3656 GN=LOC103500173 PE=4 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 7.5e-192
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Sbjct: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of IVF0009976 vs. ExPASy TrEMBL
Match: A0A0A0KNS4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177670 PE=4 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.2e-185
Identity = 336/348 (96.55%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRL+MIEKLLQKSSSDYDYRKDTFEEFYTTPLTTS+NTPTSTPFDSC
Sbjct: 61  LFYNGKLLPLHHPPRLKMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSVNTPTSTPFDSC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGE SKKSWTKKLK+STLCLKLKASR YIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGESSKKSWTKKLKYSTLCLKLKASRTYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           SFFTKSGCSDESCAAAAKIA EGSASKPNECSKKGA+VEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SFFTKSGCSDESCAAAAKIAHEGSASKPNECSKKGAKVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFS+VIKRQPIIKS S APLHPTSSSCPNISSG YLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSMVIKRQPIIKSLSSAPLHPTSSSCPNISSGNYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           GAI HCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Sbjct: 301 GAIEHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of IVF0009976 vs. ExPASy TrEMBL
Match: A0A6J1GXX8 (probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN=LOC111458502 PE=4 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.0e-165
Identity = 311/350 (88.86%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300
           GNTE+S  NRRSFSLVIKRQP IK SS A L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTESSRSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of IVF0009976 vs. ExPASy TrEMBL
Match: A0A6J1JSI7 (probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=LOC111489379 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.6e-163
Identity = 311/350 (88.86%), Postives = 323/350 (92.29%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSSDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTP--TSTPFD 120
           LFYNGKLLPLHHPPRLQM+EKL QKSSSDYD+RKDTFEEF +TPLTTS+NTP  TSTPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVEKLWQKSSSDYDFRKDTFEEF-STPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180
           SCNISPSESFRISRELN DEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASR Y
Sbjct: 121 SCNISPSESFRISRELNSDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRTY 180

Query: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300
           GNTE+SS NRRSFSLVIKRQP IK SS A L+PTSSSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 348

BLAST of IVF0009976 vs. NCBI nr
Match: XP_008461610.1 (PREDICTED: probable membrane-associated kinase regulator 4 [Cucumis melo] >KAA0062212.1 putative membrane-associated kinase regulator 4 [Cucumis melo var. makuwa])

HSP 1 Score: 679 bits (1752), Expect = 5.10e-246
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348
           GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Sbjct: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of IVF0009976 vs. NCBI nr
Match: XP_004143685.1 (probable membrane-associated kinase regulator 4 [Cucumis sativus] >KGN50499.1 hypothetical protein Csa_000573 [Cucumis sativus])

HSP 1 Score: 656 bits (1693), Expect = 5.02e-237
Identity = 336/348 (96.55%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRL+MIEKLLQKSSSDYDYRKDTFEEFYTTPLTTS+NTPTSTPFDSC
Sbjct: 61  LFYNGKLLPLHHPPRLKMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSVNTPTSTPFDSC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGE SKKSWTKKLK+STLCLKLKASR YIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGESSKKSWTKKLKYSTLCLKLKASRTYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           SFFTKSGCSDESCAAAAKIA EGSASKPNECSKKGA+VEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SFFTKSGCSDESCAAAAKIAHEGSASKPNECSKKGAKVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFS+VIKRQPIIKS S APLHPTSSSCPNISSG YLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSMVIKRQPIIKSLSSAPLHPTSSSCPNISSGNYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348
           GAI HCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG
Sbjct: 301 GAIEHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348

BLAST of IVF0009976 vs. NCBI nr
Match: XP_038893327.1 (probable membrane-associated kinase regulator 4 [Benincasa hispida])

HSP 1 Score: 629 bits (1623), Expect = 2.33e-226
Identity = 325/348 (93.39%), Postives = 334/348 (95.98%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MAVDIPSCDPSDDDYID+EIGSYTNF CCSKSSPRQSREFEFQMSLNSRGREPTTSPADE
Sbjct: 1   MAVDIPSCDPSDDDYIDMEIGSYTNFHCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTPFDSC 120
           LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPL+TS+NTPTSTPF+SC
Sbjct: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLSTSVNTPTSTPFESC 120

Query: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAYIK 180
           NISPSESFRISRELNPDEYLLEYSADVSYLNGE  KKSWTKKLKHSTLCLKLKASRAYIK
Sbjct: 121 NISPSESFRISRELNPDEYLLEYSADVSYLNGESLKKSWTKKLKHSTLCLKLKASRAYIK 180

Query: 181 SFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDKGN 240
           S FTKSGCSDESCAAAAKIADEGSASK NE SKK A+VEKRKPFGPISSSIANSNKDKGN
Sbjct: 181 SLFTKSGCSDESCAAAAKIADEGSASKANEYSKKSAKVEKRKPFGPISSSIANSNKDKGN 240

Query: 241 TENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENSIQ 300
           TENSSRNRRSFS+VIKRQP IKS S AP +P+SSSCPNISS  YLKRCGSVNSEIENSIQ
Sbjct: 241 TENSSRNRRSFSMVIKRQPNIKSPSSAPQYPSSSSCPNISSRNYLKRCGSVNSEIENSIQ 300

Query: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348
           GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQEIPEL RG
Sbjct: 301 GAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEIPELCRG 348

BLAST of IVF0009976 vs. NCBI nr
Match: XP_023527329.1 (probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 592 bits (1527), Expect = 1.02e-211
Identity = 312/350 (89.14%), Postives = 327/350 (93.43%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPT--STPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTPT  STPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTK+LK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKRLKRSTLGLKLKASRAY 180

Query: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300
           GNTE+SS NRRSFSLVIKRQP IK SS A L+PTSSSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTSSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of IVF0009976 vs. NCBI nr
Match: KAG6601024.1 (putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031828.1 putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592 bits (1525), Expect = 2.06e-211
Identity = 312/350 (89.14%), Postives = 326/350 (93.14%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNFRCCSKSSPRQSREFEFQMSLNSRGREPTTSPADE 60
           MA DIPS DPSDDDYID+EIGSYTNF C SKSSPRQSREFEFQMS+NSRGREPTTSPADE
Sbjct: 1   MAFDIPSYDPSDDDYIDMEIGSYTNFLCDSKSSPRQSREFEFQMSMNSRGREPTTSPADE 60

Query: 61  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPT--STPFD 120
           LFYNGKLLPLHHPPRLQM+E+LLQKSSSDYD+RKDTFEEF++TPLTTS+NTPT  STPFD
Sbjct: 61  LFYNGKLLPLHHPPRLQMVERLLQKSSSDYDFRKDTFEEFFSTPLTTSINTPTATSTPFD 120

Query: 121 SCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASRAY 180
           SCNISPSESFRISRELNPDEY LEYSAD SYLNGE SKKSWTKKLK STL LKLKASRAY
Sbjct: 121 SCNISPSESFRISRELNPDEYFLEYSADASYLNGESSKKSWTKKLKRSTLGLKLKASRAY 180

Query: 181 IKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNKDK 240
           IKS FTK+GCSDESCA AAKIADEGSASK NECSKKGA+VEKRKPFGPISSSI+NSNKDK
Sbjct: 181 IKSLFTKTGCSDESCAVAAKIADEGSASKANECSKKGAKVEKRKPFGPISSSISNSNKDK 240

Query: 241 GNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEIENS 300
           GNTE+SS NRRSFSLVIKRQP IK SS A L+PT SSCPN SSG YLKRCGSVNSEIENS
Sbjct: 241 GNTESSSSNRRSFSLVIKRQPNIKPSS-ASLNPTPSSCPNNSSGYYLKRCGSVNSEIENS 300

Query: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 348
           IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSS+LTVSDDQE PEL RG
Sbjct: 301 IQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSSLTVSDDQEKPELCRG 349

BLAST of IVF0009976 vs. TAIR 10
Match: AT2G39370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37380.1); Has 184 Blast hits to 178 proteins in 53 species: Archae - 0; Bacteria - 58; Metazoa - 9; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 14 (source: NCBI BLink). )

HSP 1 Score: 242.3 bits (617), Expect = 5.9e-64
Identity = 169/360 (46.94%), Postives = 225/360 (62.50%), Query Frame = 0

Query: 1   MAVDIPSCDPSDDDYIDIEIGSYTNF--RCCSKSSPRQSREFEFQMS-LNSRGREPTTSP 60
           MA  +  CD  ++DYID+E+ S+TN   +  S + P   REFEFQMS L     + TTSP
Sbjct: 1   MAAYLERCDSVEEDYIDMEVTSFTNLVRKTLSNNYP---REFEFQMSHLCPLEIDKTTSP 60

Query: 61  ADELFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTT-SLNTP--TS 120
           ADELFY GKLLPLH PPRLQM++K+L+      DY  D  +EFY+TPL T ++ TP  ++
Sbjct: 61  ADELFYKGKLLPLHLPPRLQMVQKILE------DYTFD--DEFYSTPLATGTVTTPVTSN 120

Query: 121 TPFDSCNISPSESFRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKL---KHSTLCLK 180
           TPF+SC +SP+ES ++S+ELNP++Y LEYS  +     +  KKSWT KL   K S+L  K
Sbjct: 121 TPFESCTVSPAESCQVSKELNPEDYFLEYSDSLE--EDDEKKKSWTTKLRLMKQSSLGTK 180

Query: 181 LKASRAYIKSFFTKSGCSDE-SCA-AAAKIADEGSASKPNECSKKGARVEKRKPFGPISS 240
           +KASRAY++SFF K+ CSDE SCA +AA++ADE S            R  + KPFG I +
Sbjct: 181 IKASRAYLRSFFGKTSCSDESSCASSAARVADEDSV----------LRYSRVKPFGQIKT 240

Query: 241 SIANSNKDKGNTENSSRNRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCG 300
                 K + N   S  +RRSFS+ ++RQ    S++       SS+       ++LKR  
Sbjct: 241 ---ERPKKQSNGSVSGSHRRSFSVSMRRQAAKSSNN------KSSNSLGFRPLQFLKRST 300

Query: 301 SVNSEIENSIQGAIAHCKQSQSQ-QPLRSRKTVSEVGIYLLSSSTLTVSDDQEIPELGRG 349
           S +SEIENSIQGAI HCKQSQ Q Q  +   TV+EVG   LS+S +   DDQE  ++ RG
Sbjct: 301 SSSSEIENSIQGAILHCKQSQQQKQKQKQYSTVNEVGFCSLSASRIAARDDQEWAQMFRG 328

BLAST of IVF0009976 vs. TAIR 10
Match: AT2G37380.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G39370.1); Has 1284 Blast hits to 422 proteins in 114 species: Archae - 0; Bacteria - 90; Metazoa - 125; Fungi - 151; Plants - 136; Viruses - 0; Other Eukaryotes - 782 (source: NCBI BLink). )

HSP 1 Score: 129.8 bits (325), Expect = 4.3e-30
Identity = 131/350 (37.43%), Postives = 176/350 (50.29%), Query Frame = 0

Query: 12  DDDYIDIEI----------GSYTNFRCCSKSSPRQSREFEFQMSLNS-RGREPTTSPADE 71
           DD YID+E+           S + F     SSP QSREFEFQM  ++    E TTSPADE
Sbjct: 18  DDGYIDMEVNLSSSSSSSTSSSSFFSFPVTSSPPQSREFEFQMCSSAVASGESTTSPADE 77

Query: 72  LFYNGKLLPLHHPPRLQMIEKLLQKSSSDYDYRKDTFEEFYTTPLTTSLNTPTSTP--FD 131
           LFY G+LLPLH PPRL+M++KLL  SSS     +        TP++        +P  F 
Sbjct: 78  LFYKGQLLPLHLPPRLKMVQKLLLASSSSTAATE--------TPISPRAAADVLSPRRFS 137

Query: 132 SCNISPSES--FRISRELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKLKASR 191
           SC I   E+  F IS EL   +  +E        N      SW+KK+KHS++  KLKASR
Sbjct: 138 SCEIGQDENCFFEISTEL---KRFIE-------SNENHLGNSWSKKIKHSSITQKLKASR 197

Query: 192 AYIKSFFTKSGCSDESCAAAAKIADEGSASKPNECSKKGARVEKRKPFGPISSSIANSNK 251
           AYIK+ F+K  CSD S        +     +P++ S+      K+ PF            
Sbjct: 198 AYIKALFSKQACSDSS------EINPRFKIEPSKVSR------KKNPF------------ 257

Query: 252 DKGNTENSSR-NRRSFSLVIKRQPIIKSSSFAPLHPTSSSCPNISSGKYLKRCGSVNSEI 311
              N+EN    +RRSFS VI+R    K S+ +    +SSS  ++SS       GS++ + 
Sbjct: 258 --VNSENPLLIHRRSFSGVIQRHSQAKCSTSS---SSSSSASSLSSSFSFGSNGSLDLQT 313

Query: 312 --------ENSIQGAIAHCKQSQSQQPLRSRKTVSEVGIYLLSSSTLTVS 338
                   +NSI+GAI HCKQS + +  +S  T SE     L SS  +VS
Sbjct: 318 LMRSSNASDNSIEGAIEHCKQSFTTR--KSNVTESE-----LCSSRTSVS 313

BLAST of IVF0009976 vs. TAIR 10
Match: AT5G26230.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 1.6e-05
Identity = 94/335 (28.06%), Postives = 134/335 (40.00%), Query Frame = 0

Query: 30  SKSSP-RQSREFEFQMSLNSRGREPTTSPADELFYNGKLLPLHHPPRLQMIEKL-LQKSS 89
           S SSP   S EFEF +S++ R    +  PADELFY G+LLPL   PRL ++  L    SS
Sbjct: 15  SHSSPSSSSSEFEFNISISPRKASSSLCPADELFYKGQLLPLQLSPRLSLVRTLGSSTSS 74

Query: 90  SDY----------DYRKDTFEEFYTTPLTTSLNTPTSTPFDSC----------------- 149
           SDY             +D+ E   +T  T S       P D C                 
Sbjct: 75  SDYTSSSSSSVATSAARDSTESNSSTDSTASFPLLHPPPLDCCDSSRPSSVTDDEDFFFK 134

Query: 150 ---NISPSESFRISR-----ELNPDEYLLEYSADVSYLNGERSKKSWTKKLKHSTLCLKL 209
              N S S  F +SR     + +P   L  +S+  S      +  S   K   ST    +
Sbjct: 135 PPKNKSSSGGFSLSRFSSVFKKDPKTNLHHHSSSSSTATTAAAPSS--VKRMSSTAKEVI 194

Query: 210 KASRAYIKSFFTKSGCSDESCAAAAKIADEGSAS-KPNECSKKGARVEKRKPFGP--ISS 269
           +     +K  + K      S   ++ I  E S+S K +  + +G          P  +SS
Sbjct: 195 RKYMKKVKPLYEKL-----SPKQSSNIKTESSSSLKDSGNNIRGTTTVTTVTAAPTVVSS 254

Query: 270 SIANSNKDKGNTENSSRNRR---SFSLVIKRQP----IIKSSSFAPLHPTSSSCPNISSG 318
               S    GN    ++  R   S    ++  P    ++    F P+H    SC + SS 
Sbjct: 255 GGGLSISFSGNLMKYTKRGRCAASCPSSMRSSPNHSGVLTRGGF-PVH--QGSCSSSSSN 314

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O806248.3e-6346.94Probable membrane-associated kinase regulator 4 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9ZUS86.0e-2937.43Probable membrane-associated kinase regulator 3 OS=Arabidopsis thaliana OX=3702 ... [more]
Q3E9362.3e-0428.06Probable membrane-associated kinase regulator 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A5A7V1W47.5e-192100.00Putative membrane-associated kinase regulator 4 OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3CEW17.5e-192100.00probable membrane-associated kinase regulator 4 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A0A0KNS45.2e-18596.55Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G177670 PE=4 SV=1[more]
A0A6J1GXX86.0e-16588.86probable membrane-associated kinase regulator 4 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JSI79.6e-16388.86probable membrane-associated kinase regulator 4 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
XP_008461610.15.10e-246100.00PREDICTED: probable membrane-associated kinase regulator 4 [Cucumis melo] >KAA00... [more]
XP_004143685.15.02e-23796.55probable membrane-associated kinase regulator 4 [Cucumis sativus] >KGN50499.1 hy... [more]
XP_038893327.12.33e-22693.39probable membrane-associated kinase regulator 4 [Benincasa hispida][more]
XP_023527329.11.02e-21189.14probable membrane-associated kinase regulator 4 [Cucurbita pepo subsp. pepo][more]
KAG6601024.12.06e-21189.14putative membrane-associated kinase regulator 4, partial [Cucurbita argyrosperma... [more]
Match NameE-valueIdentityDescription
AT2G39370.15.9e-6446.94unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT2G37380.14.3e-3037.43unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G26230.11.6e-0528.06unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..249
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 227..249
NoneNo IPR availablePANTHERPTHR33312:SF21MEMBRANE-ASSOCIATED KINASE REGULATOR 3-RELATEDcoord: 1..344
IPR039620BKI1/Probable membrane-associated kinase regulator 1/3/4PANTHERPTHR33312MEMBRANE-ASSOCIATED KINASE REGULATOR 4-RELATEDcoord: 1..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009976.1IVF0009976.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0016310 phosphorylation
cellular_component GO:0005886 plasma membrane
molecular_function GO:0016301 kinase activity
molecular_function GO:0019210 kinase inhibitor activity