IVF0009897 (gene) Melon (IVF77) v1

Overview
NameIVF0009897
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionSteroid 5-alpha-reductase DET2
Locationchr04: 31580209 .. 31581586 (-)
RNA-Seq ExpressionIVF0009897
SyntenyIVF0009897
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATTAAATTTCTATCTCGTGAAGAATAAAAAAAGTGATTTTTTTTCCTTACTTGATTTTGAAATGTTCTTAACATTTTATATATTCAACATCTTTCCTCATCTATAAAAAGAAATACAAAAAAGAGAGAAACACCCAGTCATCTCTTTCCAAATTGAAAAAGGATAATTACTCTAATGAAAAATAGAGAATAAACCTTCTCCCCTTTTATTATATAGTTTTAGATCCATGGATTACATATTGAATAATGATTATTGATTTTCACCCTTTAATTTTACGGAACTTTATTAATCGAAATTACTCCACAAAGTCACCAAAAAGTGTCCCAAGTCTATTACCATAGTGGAGAAGGTGACTTCACTATGAGATTCAATAGGCTTCCTTCCCTCTGTCAGCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAACTCTATGTCTATCATATTTCATATTAAAATAAAATAAATTCCTTTTGCCTTGTTTTAAAAGGGAATTTTTTTTT

mRNA sequence

GAATTAAATTTCTATCTCGTGAAGAATAAAAAAAGTGATTTTTTTTCCTTACTTGATTTTGAAATGTTCTTAACATTTTATATATTCAACATCTTTCCTCATCTATAAAAAGAAATACAAAAAAGAGAGAAACACCCAGTCATCTCTTTCCAAATTGAAAAAGGATAATTACTCTAATGAAAAATAGAGAATAAACCTTCTCCCCTTTTATTATATAGTTTTAGATCCATGGATTACATATTGAATAATGATTATTGATTTTCACCCTTTAATTTTACGGAACTTTATTAATCGAAATTACTCCACAAAGTCACCAAAAAGTGTCCCAAGTCTATTACCATAGTGGAGAAGGTGACTTCACTATGAGATTCAATAGGCTTCCTTCCCTCTGTCAGCTTCTTCCTTTTAGGTATGTAGATCTGGATTTTAATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCATAAATAGGAAGGCTGTAATTCCCTTCTTGTATTAACTCTATGTCTATCATATTTCATATTAAAATAAAATAAATTCCTTTTGCCTTGTTTTAAAAGGGAATTTTTTTTT

Coding sequence (CDS)

ATGAGATATGTTACAAATTTGAAAACAGATCAGCCACAAATTTCTCAACTTGATTCAAACCTACAACCGGAATCCTCGAATCCATCGCCTACCGTGATGGGTTGGGATCAGGCTCTGTTCCGCAACAGTCTTCTCGCCCTGTACCTCATTGGACCACCGACCGTCGTCTCCCTGAGGTTTCTCCAGGCGCCGTACGGGAAGCACATCCGACCCGGGTGGGGTCCAACCATGTCGGCTCCATTGGCCTGGTTCCTGATGGAAAGTCCCACCTTATGGTTGACCCTCCTCCTCTTTCCATCCGGCGATCACTCCTCCGATTCCAAGGCTCTCCTTCTCATCTCCCCCTTCCTCCTTCACTACCTCAATCGCACCTGCTTCTACCCAATTCGTCTTCTCTGGAGTGGGACTCGACGGAATGCCACCGCCGGTTTTCCGGTGATTGTGGCTATCATGGCATTCGGATTCAACTTGCTCAATGCTTACGTGCAGACCAGATCTGTGTCTCATTACGTGAAGTACGAGGGAGATGGGTGGTTTTGGTGGCGGTTTTTTGGTGGAGGAGTGGTTTTTGTGATGGGGATGGCGGTGAATATATGGTCGGATGGTGTTCTGGTCGGACTGAAGAGGAGCGGCGGCGGTTATAAGGTGCCGAAGGGAGGGTGGTTTGAGTTGGTGAGTTGTCCTAACTATTTGGGAGAGATTATGGAGTGGCTGGGCTGGGGGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTCCTTTATACGTGTGCAAATTTAGTGCCTAGGGCACGTGCAAACCACACGTGGTACTTGAACAAGTTTGGAGAGGATTACCCATAA

Protein sequence

MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP
Homology
[Close]
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 7.3e-94
Identity = 167/250 (66.80%), Postives = 187/250 (74.80%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF   LL LY+I  PT +SL FLQAPYGKH RPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LT  LFPSG H  + K+ LLISPFL HY NRT  YP+RL  + T+   T GFPV VA MA
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRLARNTTQ---TRGFPVSVAFMA 120

Query: 153 FGFNLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG 212
           FGFNLLN Y+Q R VSHY   YE +  FWWRF  G ++FV+GM VN+ +D VLVGLK+ G
Sbjct: 121 FGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQG 180

Query: 213 -GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTW 272
            GGYK+P+GG FELVSCPNY GEIMEW GW VM  SW G GFFLYTCANL+PRARA   W
Sbjct: 181 DGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLW 240

Query: 273 YLNKFGEDYP 281
           YL KF +DYP
Sbjct: 241 YLEKFKDDYP 247

[Close]
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 331.3 bits (848), Expect = 1.1e-89
Identity = 155/247 (62.75%), Postives = 182/247 (73.68%), Query Frame = 0 

Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  FR  LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF F
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTF 125

Query: 156 NLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GG 215
           NLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   GG
Sbjct: 126 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 185

Query: 216 YKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLN 275
           Y +P+GGWFELVSCPNY GE +EWLGW VM  SWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 186 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 245

Query: 276 KFGEDYP 281
           KF E+YP
Sbjct: 246 KFKEEYP 251

[Close]
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 8.7e-79
Identity = 140/246 (56.91%), Postives = 175/246 (71.14%), Query Frame = 0 

Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPG-WGPTMSAPLAWFLMESPTLWLT 95
           D+ LF   +  + ++  PT +  +F  +PYGKH      G T+S P+AW  MESPTLWLT
Sbjct: 5   DENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPTLWLT 64

Query: 96  LLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFG 155
           +++F  G + ++  A LLISP+L HY NRT  YP+RL    T+ N    FP+ +A+ AF 
Sbjct: 65  IIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLRLRSRNTKNN----FPLNIAVTAFI 124

Query: 156 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGY 215
           FNLLNAY+Q+R VSHY  Y+ D WFW RF  G V+F  GM +NIW+DGVL+GLK  GGGY
Sbjct: 125 FNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQGGGY 184

Query: 216 KVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNK 275
           K+P+GG F+ VS PNYLGEIMEWLGW +M  SWAGL FF+YTCANLVPRA +NH WYL K
Sbjct: 185 KIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKWYLQK 244

Query: 276 FGEDYP 281
           FGEDYP
Sbjct: 245 FGEDYP 246

[Close]
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 137.9 bits (346), Expect = 1.8e-31
Identity = 84/233 (36.05%), Postives = 132/233 (56.65%), Query Frame = 0 

Query: 54  TVVSLRFLQAPYGKHIRPGW-GPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKAL 113
           ++V LR + +PYG++  P W G  + A  AWF+ E P++ W L   + P+     +    
Sbjct: 27  SIVGLRSVGSPYGRY-SPQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86

Query: 114 LLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVA--IMAFGFNLLNAYVQTRSVS 173
           +L++ FL+HY+ RT  +P+ +           G P ++   ++AF F   N YVQ+R +S
Sbjct: 87  VLLAMFLIHYVQRTLVFPVLI---------RGGKPTLLVTFVLAFLFCTFNGYVQSRYLS 146

Query: 174 HYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GGYKVPKGGWFELVSC 233
            +  Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P+GG FE VS 
Sbjct: 147 QFAVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSA 206

Query: 234 PNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
            NY GE++EW G+ +   S  G+ F L+T + L+ RA+ +H WY  KF EDYP
Sbjct: 207 ANYFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYP 248

[Close]
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 134.8 bits (338), Expect = 1.5e-30
Identity = 83/230 (36.09%), Postives = 127/230 (55.22%), Query Frame = 0 

Query: 56  VSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPSGDHSSDSKALLLI 115
           V L+ + + YG++     G  + A  AWFL E P++ W L   + P+     +    +L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 116 SPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIV--AIMAFGFNLLNAYVQTRSVSHYV 175
           + FL+HY+ RT  +P+ +           G P ++   ++AF F  LN Y+Q+R +S + 
Sbjct: 85  AMFLIHYVQRTLVFPVLI---------RGGKPTLLFTFVLAFLFCTLNGYLQSRYLSQFA 144

Query: 176 KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GGYKVPKGGWFELVSCPNY 235
            Y  D      F  G  ++++GM +NI SD +L  L++ G  GYK+P+GG FE VS  NY
Sbjct: 145 VYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANY 204

Query: 236 LGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
            GE++EW G+ +   S  G+ F L+T   L  RAR +H WYL KF EDYP
Sbjct: 205 FGELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYP 244

[Close]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.0e-167
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0 

Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
           GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 280

[Close]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 1.1e-158
Identity = 266/280 (95.00%), Postives = 270/280 (96.43%), Query Frame = 0 

Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YP
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYP 280

[Close]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 281
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 5.8e-126
Identity = 212/248 (85.48%), Postives = 226/248 (91.13%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT +SLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           F FNLLN+Y+QTRSVSHY+ Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK +GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 281
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match: A0A6J1F1U8 (Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.7e-125
Identity = 211/248 (85.08%), Postives = 225/248 (90.73%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV++A+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVMALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           F FNLLN+Y+QTRSVSHY+ Y+ D WFWWRFFGG VVF++GMAVNIWSDGVLV LK +GG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFLLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIAEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 281
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 595 bits (1533), Expect = 9.35e-215
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0 

Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF
Sbjct: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 280
           GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP
Sbjct: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 280

[Close]
BLAST of IVF0009897 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 566 bits (1459), Expect = 1.79e-203
Identity = 266/280 (95.00%), Postives = 270/280 (96.43%), Query Frame = 0 

Query: 1   MRYVTNLKTDQPQISQLDSNLQPESSNPSPTVMGWDQALFRNSLLALYLIGPPTVVSLRF 60
           MR+ TNLKTD PQISQLDSNLQPESSNPSPT+MGWDQALF NSLLALYLIGPPTVVSLRF
Sbjct: 1   MRHGTNLKTDPPQISQLDSNLQPESSNPSPTMMGWDQALFHNSLLALYLIGPPTVVSLRF 60

Query: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSDSKALLLISPFLLHY 120
           LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSS SKALLLISPFLLHY
Sbjct: 61  LQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTLLLFPSGDHSSHSKALLLISPFLLHY 120

Query: 121 LNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGFNLLNAYVQTRSVSHYVKYEGDGWFW 180
           LNRTCFYPIRLLWSGTRRNA  GFPV+VAIMAFGFNLLN+YVQTRSVSHYVKYEGDGWFW
Sbjct: 121 LNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMAFGFNLLNSYVQTRSVSHYVKYEGDGWFW 180

Query: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGGGYKVPKGGWFELVSCPNYLGEIMEWLGW 240
           WRFFGGGVVFVMGMAVNIWSDGVLVGLKR GGGYKVP GGWFELVSCPNYLGEIMEWLGW
Sbjct: 181 WRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGGGYKVPTGGWFELVSCPNYLGEIMEWLGW 240

Query: 241 GVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 280
            VMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGE YP
Sbjct: 241 AVMCGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEVYP 280

[Close]
BLAST of IVF0009897 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 533 bits (1372), Expect = 1.01e-190
Identity = 248/248 (100.00%), Postives = 248/248 (100.00%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 280
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 476 bits (1224), Expect = 3.65e-168
Identity = 220/248 (88.71%), Postives = 232/248 (93.55%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           MG DQALF NSLL LYLIGP  VV+LRFLQAPYGKH +PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGDHSS+SKALLLISPFLLHY NRTC YPIRLL SGTRRN  AGFPVIVA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLN+Y+QTRSVSHY++YEGDGWFWWRFFGGGVVF++GM+VNIWSDGVLVGLKR GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP G WFELVSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 280
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 462 bits (1189), Expect = 7.85e-163
Identity = 215/248 (86.69%), Postives = 227/248 (91.53%), Query Frame = 0 

Query: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92
           M  DQ LF NSLLALYLIGPPT VSLRFLQAPYGKH RPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152
           LTLLLFPSGD+SS+ K+LLLIS FLLHYLNRTC YPIRLL SGTRRN+  GFPV+VA+MA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212
           FGFNLLN+Y+QTRSVSHYV Y+ D WFWWRFFGG VVFV+GMAVNIWSDGVLV LK +GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272
           GYKVP GGWFELVSCPNYLGEI+EWLGW VMCGSWAGLGFFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 273 NKFGEDYP 280
           NKFGEDYP
Sbjct: 241 NKFGEDYP 248

[Close]
BLAST of IVF0009897 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 331.3 bits (848), Expect = 7.8e-91
Identity = 155/247 (62.75%), Postives = 182/247 (73.68%), Query Frame = 0 

Query: 36  DQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLWLTL 95
           D+  FR  LL L   GPPT V L+FLQAPYGKH R GWGPT+S P+AWF+MESPTLWLTL
Sbjct: 6   DKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLTL 65

Query: 96  LLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMAFGF 155
           LLFP G H+ + K+LLL SP+L+HY +RT  YP+RL  S        GFP+ +A +AF F
Sbjct: 66  LLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN-GFPITIAALAFTF 125

Query: 156 NLLNAYVQTRSVSHYV-KYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSG-GG 215
           NLLN Y+Q R VSHY   YE   WFWWRF  G VVF+ GM +NI SD  LV LK+   GG
Sbjct: 126 NLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENRGG 185

Query: 216 YKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYLN 275
           Y +P+GGWFELVSCPNY GE +EWLGW VM  SWAG+GFFLYTC+NL PRARA+H WY+ 
Sbjct: 186 YVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWYIA 245

Query: 276 KFGEDYP 281
           KF E+YP
Sbjct: 246 KFKEEYP 251

[Close]
BLAST of IVF0009897 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 68.2 bits (165), Expect = 1.2e-11
Identity = 35/97 (36.08%), Postives = 60/97 (61.86%), Query Frame = 0 

Query: 186 GGVVFVMGMAVNIWSDGVLVGLKRSGG--GYKVPKGGWFELVSCPNYLGEIMEWLGWGVM 245
           G V+FV+G+  N++   +L  L++  G   YK+PKGG F+++ CP+YL EI+ +  + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 246 CGSWAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
             +     F + T   L+ R+ A  TWYL+KF +D+P
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFP 257

[Close]
BLAST of IVF0009897 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.5e-09
Identity = 38/103 (36.89%), Postives = 50/103 (48.54%), Query Frame = 0 

Query: 186 GGVVFVMGMAVNIWSDGVLVGLKR---SGGGYKVPKGGWFELVSCPNYLGEIMEWLGWGV 245
           GG +F+ G         +L  L+        Y +P G WFE+VSCP++L EI+ +LG  +
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLI 270

Query: 246 MCGS-----WAGLGFFLYTCANLVPRARANHTWYLNKFGEDYP 281
             G      W   GF     ANL   A   H WYL KF E+YP
Sbjct: 271 SSGGTDISIWLLFGF---VAANLTYAAGETHRWYLQKF-ENYP 309

[Close]
BLAST of IVF0009897 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 43/134 (32.09%), Postives = 59/134 (44.03%), Query Frame = 0 

Query: 155 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS---G 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 215 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 274

Query: 215 GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 275 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 332

Query: 275 NHTWYLNKFGEDYP 281
            H WYL KF E+YP
Sbjct: 335 THRWYLRKF-ENYP 332

[Close]
BLAST of IVF0009897 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 43/134 (32.09%), Postives = 59/134 (44.03%), Query Frame = 0 

Query: 155 FNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRS---G 214
           FNLL+      S+S  +K     W       GG +F+ G         +L  L+ +    
Sbjct: 214 FNLLS------SISPLMKLGSLQWI------GGAIFLWGWIHQRRCHAILGSLRENPSQA 273

Query: 215 GGYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGS-----WAGLGFFLYTCANLVPRARA 274
             Y +P G WF +VS P++L EI+ + G  +  G      W   GF     ANL   A  
Sbjct: 274 KEYIIPYGDWFGMVSSPHFLAEIVLYAGLLIASGGTDITIWLLFGF---VAANLTYAAGE 331

Query: 275 NHTWYLNKFGEDYP 281
            H WYL KF E+YP
Sbjct: 334 THRWYLRKF-ENYP 331

The following BLAST results are available for this feature:
BLAST of IVF0009897 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q2QDF67.3e-9466.80Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1[more]
Q389441.1e-8962.75Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2[more]
Q5K2N18.7e-7956.91Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1[more]
P240081.8e-3136.053-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ... [more]
Q68FF91.5e-3036.093-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ... [more]
BLAST of IVF0009897 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3B6E33.0e-167100.00Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1[more]
A0A0A0LAB31.1e-15895.00Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S... [more]
A0A5D3DNY21.1e-148100.00Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A6J1J7F25.8e-12685.48Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ... [more]
A0A6J1F1U83.7e-12585.08Steroid 5-alpha-reductase DET2 OS=Cucurbita moschata OX=3662 GN=LOC111441400 PE=... [more]
BLAST of IVF0009897 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008442471.19.35e-215100.00PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo][more]
XP_004137737.11.79e-20395.00steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei... [more]
KAA0044147.11.01e-190100.00steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-... [more]
XP_038905208.13.65e-16888.71LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida][more]
XP_023527474.17.85e-16386.69steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo][more]
BLAST of IVF0009897 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G38050.17.8e-9162.753-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT5G16010.11.2e-1136.083-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT1G72590.11.5e-0936.893-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G16530.11.8e-0732.093-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
AT2G16530.21.8e-0732.093-oxo-5-alpha-steroid 4-dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPFAMPF02544Steroid_dhcoord: 144..280
e-value: 1.4E-40
score: 138.7
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 186..265
score: 26.181702
IPR0166363-oxo-5-alpha-steroid 4-dehydrogenasePIRSFPIRSF0155965_alpha-SR2coord: 31..280
e-value: 6.0E-78
score: 259.6
NoneNo IPR availableGENE3D1.20.120.1630coord: 109..280
e-value: 8.1E-11
score: 44.0
NoneNo IPR availablePANTHERPTHR10556:SF53STEROID 5-ALPHA-REDUCTASE DET2coord: 35..280
IPR0393573-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductasePANTHERPTHR105563-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 35..280

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009897.2IVF0009897.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0008202 steroid metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003865 3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function GO:0047751 cholestenone 5-alpha-reductase activity
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors