IVF0009648 (gene) Melon (IVF77) v1

Overview
NameIVF0009648
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr02: 3148836 .. 3152246 (+)
RNA-Seq ExpressionIVF0009648
SyntenyIVF0009648
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTCAATCGGAAAACTGCCTCTGGGACCCTACGGAGCAAAATTTTGTTGCTCAGGGTCCTCTCGTTCTCTCTCTCTCTCTCTCTCTCCGCCCTTGATCCAAATCTTCATTCACTGTTCTTCTTTCTCCTTCTTCCCTAACCTTCCCACTCCTCCTACTTCAGCTCCGGTACGTCTTTCTTCACTTCAATTTCAATTTAGGGTTCCATTTTTCAATTTCTGCTTCTTCCTAATTTTGTTTTGATACCATATTCATATAGTTCTTCTTGATTTTATATCTGTCCTAATTCTGCTTAATTTCGATGTTGTTTCAAGTGTTTTTTTTGCAATTCTTTCCATAAATCTCAACCGGACGAGAGTTTGAACGCCTCGTTTGAGTGATGATTCTGTAACGAGGGAAATTTTCCTCTCGGTTATAGGAGACAACTCAAAAATAAATTGGGATTAGGTCGGATTGACCAATCCCACTCGAATTACATATGTATATATAAACAATATATATGCATTTCTTTTAAAATGGATGACTTTTTAATGTTTTTTTTAGATATCATGGATATTTGAACATTTAAGTTTTATAAGATTTTAGTTTAAATTCATGTATTTTTTAGAAATCCTGTTGGAACTGGGAATCTTTTGGATTGCCAACTCAACCAACATGATTTATGATTTTTCGGGTTGGTCCAAAAATTTCCCACAACTCAACTTAAGTACACCCTGAACTGTCTCCTTAGCTTGTGAGAGATAAAGAATGTGGGGATTGTACAGGTGATACATCAAGAAGTTTGTCAGAAGAATTGGGGACAGTGATTGTACTCGTATTAATTCCTGGCTATCAAGTTGAAATTATGGGGTTGTTAGGCTATTCGAATTTAGTTGTGTTTATATAAGCCACATGAATGTGTTGGGATTAGTCATTTTTTATTATTTATATTGCATTTTAGTCTATATGTTTCCATTTTTGTTCTTAGTTTTCTTTTATTTTACCAAATAGTCTTACAAATAATCAAATGTAACATGTCCAATTTAGTAAGATGCATTTTTCAACCATAATTGTACTTAAAATCTGACCCCTGTTAAGACATAAAGTCAATAGAATATTCTTTGAAATTATGTATGTATTTACCTTGATTGATTTTTGCCAATATGGCATGATTCCGATTATTTTCCGTATGTAATAATTCTCAATGTCTCTACTATTCTGGTTCATTATAAAAACCATTCTCCTCTCTTGGCAAATGCACATGCCGAGAGCAACATTATCTAAACCAACCCCTTATTTTAAACCTTTTCTGTATGCTACAACGGAGTTGGGTTATCTTCCACTACATTGGGGGATTTTTGATAACCTTTAATCAAACAAACATTTTTTAAAGTACACGAATCATATATAGGTGAAAATATCAAGAGTACAATGAAAATTAATTCTGCTTTTCTCTTGAATTGTGACTGTCCATTATCCAATCAAGGAGTATATTCCTAGATTCCTTTCCAGACTAATTTGATGTGGCTTGCTTGGCTGGTAAAGTTTATACAACATCAATTCTTTTTTATATTCCGGGTTTATATCTATCTATCTATATATATATATATATATATTTTTTTTTTGGGGGGGGGGGGGGGGGGGGAATTTTAACCTTTTCTGCTTTTCATCTTTTAGTGAAATCATCACCAGATATTTGTTGACTTTAAGGAAATATATTGAAATAAACTAAGAGCCTAATTTCTAACCATTTGGTTTTTTAGGCTTGTTTCACACCAAAACTTTAGAACGATTTTCATCATTCCTAAACGAAACAAATTACGTGACAGGAAAACTCGAATGTACAAGTAGTTCTTTATAAGCTCAATCTCCAAAAACCAAACGGTTATCTAACAGGTTAGACCATTATGTGCTTTCCAAAAGTTGTATTGGGATGAATTGTTGTTATGCGTCAGCTTTTTTCACATACATTAGTCTATTGTGGATAAATGTTTGATTTTGTCTTCAAAATTGATTTTCTTAGCCAATAGGCATTTCCTTGCAGAATTCATACATTTAGGAATAAGCCTGGGCTTTTTTCTTTTAACTGTAGCAATTGAGAGAAGGATGTCGTCTGTTGGTGAAGACACCCATTGGTGCTATCAATGCAACCATTCATTCTGGCTTGATGGAGAAGATGTAGTTTGCCCTCACTGTAATGGTGGTTTTGTCGAAGAACTTAACGACGATCATGATGAAACAATTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACTCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGTCGAAGAAATCCTTATCCGAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCGGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCCGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAATTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTTGGTAATGTCACTCCAGAAGGTAGATCGAGTCAGCATACAGGACTGGAAGAACTAGCTGCACAACTTTCATTAAACGAACAAAGAGAACCAGTTCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCTCGGACTCTCATTGTCCAGTTTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGCAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTAGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAATGGTGGCAGCAACAGTGAAGACCTAAATGAGAGAGAGATGACTCAACGAAACCTGAGAAGGAGGCATCCATTCCCAGATTTTTTTGCTCCTCGTCGAATTGTCACTCGTTTTGGCAGTGGGATTCATATAAACGGCTTATCTTCAGATCGAGATCATGATAACGAGATGGGTTACCCTGAATGATGACCTTTCGATTACCAAATCTATAAATCTTCTCTACTTAAGAAGCTGCCGAGCATGATATGTGAAAGAAAGCCGTGTTTTACAACAAATGAGGTAATAAGAAATAAGAATCAGCTCTAGACTTGTAATATCTAGAGCTGTTTTCTCTTCCCCCTCTTTTTTTTTTTTTTTTCCCTTCCCTTCTTTTGTGTAAAATGATATGGAAAAAAATCTCATATCTCCTACAATGACTTGTACTGGGAAAAGGGGGTCTTAGAGATGAAATCAAATGTAATTATAGTTGATATGTTCCATGGATCAACTGAAGCCATTTGCCTTTTCTGATTTGTGAGCTTCAATGATGAGAAAAGAAGATGACATTGAAAAGGAGCAATAGATTTTATTGTGTTTATAT

mRNA sequence

AGTCAATCGGAAAACTGCCTCTGGGACCCTACGGAGCAAAATTTTGTTGCTCAGGGTCCTCTCGTTCTCTCTCTCTCTCTCTCTCTCCGCCCTTGATCCAAATCTTCATTCACTGTTCTTCTTTCTCCTTCTTCCCTAACCTTCCCACTCCTCCTACTTCAGCTCCGGAAAACTCGAATGTACAAGTAGTTCTTTATAAGCTCAATCTCCAAAAACCAAACGGTTATCTAACAGCAATTGAGAGAAGGATGTCGTCTGTTGGTGAAGACACCCATTGGTGCTATCAATGCAACCATTCATTCTGGCTTGATGGAGAAGATGTAGTTTGCCCTCACTGTAATGGTGGTTTTGTCGAAGAACTTAACGACGATCATGATGAAACAATTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACTCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGTCGAAGAAATCCTTATCCGAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCGGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCCGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAATTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTTGGTAATGTCACTCCAGAAGGTAGATCGAGTCAGCATACAGGACTGGAAGAACTAGCTGCACAACTTTCATTAAACGAACAAAGAGAACCAGTTCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCTCGGACTCTCATTGTCCAGTTTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGCAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTAGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAATGGTGGCAGCAACAGTGAAGACCTAAATGAGAGAGAGATGACTCAACGAAACCTGAGAAGGAGGCATCCATTCCCAGATTTTTTTGCTCCTCGTCGAATTGTCACTCGTTTTGGCAGTGGGATTCATATAAACGGCTTATCTTCAGATCGAGATCATGATAACGAGATGGGTTACCCTGAATGATGACCTTTCGATTACCAAATCTATAAATCTTCTCTACTTAAGAAGCTGCCGAGCATGATATGTGAAAGAAAGCCGTGTTTTACAACAAATGAGGTAATAAGAAATAAGAATCAGCTCTAGACTTGTAATATCTAGAGCTGTTTTCTCTTCCCCCTCTTTTTTTTTTTTTTTTCCCTTCCCTTCTTTTGTGTAAAATGATATGGAAAAAAATCTCATATCTCCTACAATGACTTGTACTGGGAAAAGGGGGTCTTAGAGATGAAATCAAATGTAATTATAGTTGATATGTTCCATGGATCAACTGAAGCCATTTGCCTTTTCTGATTTGTGAGCTTCAATGATGAGAAAAGAAGATGACATTGAAAAGGAGCAATAGATTTTATTGTGTTTATAT

Coding sequence (CDS)

ATGTCGTCTGTTGGTGAAGACACCCATTGGTGCTATCAATGCAACCATTCATTCTGGCTTGATGGAGAAGATGTAGTTTGCCCTCACTGTAATGGTGGTTTTGTCGAAGAACTTAACGACGATCATGATGAAACAATTCAAAATGACTTTAATCCAGGCATAGAAGAAGATCTTTCAACTCAGGTGCCTCCAATTTTCGAAGCTATGTTTGCATTAATGGGTCGAAGAAATCCTTATCCGAGATTTGGTCTTCTCGAAGCTGTTGATACTTTCACCAGAGAGAGAATGGCGGGAAGAAATCCTAACTTTGACGTCAGGAGAAGATCCGGTTCTGTTCCAGGGCAGAATTTGGATTTTTTCAATTCTTTTTGGTCATTCCATGATCACATGTCGGGTTCGACCTTTGGTAATGTCACTCCAGAAGGTAGATCGAGTCAGCATACAGGACTGGAAGAACTAGCTGCACAACTTTCATTAAACGAACAAAGAGAACCAGTTCCAACTCCTGCTTCTCACTCTTGTATAGAAGCAATGCCGACAATCAAGATTAACCAAATGCACCTCGGCTCGGACTCTCATTGTCCAGTTTGTAAAGAAAAATTTGAGCTCGAATCTGAAGCCAAAGCTTTGCCATGCAATCATATTTATCACACTGATTGCATTCTTCCTTGGCTGGTCCAACATAATACTTGCCCGGTTTGTAGACTCGAGCTGCCTCAGCAGGAAAGTGGACACAGTTGGGGAGGGAATGGTGGCAGCAACAGTGAAGACCTAAATGAGAGAGAGATGACTCAACGAAACCTGAGAAGGAGGCATCCATTCCCAGATTTTTTTGCTCCTCGTCGAATTGTCACTCGTTTTGGCAGTGGGATTCATATAAACGGCTTATCTTCAGATCGAGATCATGATAACGAGATGGGTTACCCTGAATGA

Protein sequence

MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLSTQVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPTIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDRDHDNEMGYPE
Homology
BLAST of IVF0009648 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 2.9e-43
Identity = 111/299 (37.12%), Postives = 147/299 (49.16%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS+  +THWC++C  + WL   D VC +C GGFVEE++       ++     +E D   
Sbjct: 1   MSSI-RNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRD-----VERD--- 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
                              P F L+EA   F R R+A R+ + ++  R GS   ++    
Sbjct: 61  -------------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNL 120

Query: 121 NSFWSF----------HDHMSGSTFGNVTPEGRSSQH----------TGLEELAAQLSLN 180
                F           D+ S   F N    G                GLEEL  QLS  
Sbjct: 121 APLLIFGGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 180

Query: 181 EQREPVPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTD 240
                 P PA  S I+A+PTIKI Q HL  SDSHCPVCK++FEL+SEAK +PC+HIYH+D
Sbjct: 181 THHRG-PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 240

Query: 241 CILPWLVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFF 279
           CI+PWLVQHN+CPVCR ELP +       G+  S     N     + N RRR+ F + +
Sbjct: 241 CIVPWLVQHNSCPVCRKELPSR-------GSSSSTQSSQNRSTNGRENSRRRNIFSNLW 263

BLAST of IVF0009648 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.2e-42
Identity = 122/332 (36.75%), Postives = 163/332 (49.10%), Query Frame = 0

Query: 3   SVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLSTQV 62
           S   +THWC++C  +  L G++ VC +C GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHM--------------SGSTFGNVTPEGRSSQH---TGLEELAAQLSLNEQREP 182
              F   +              +G + G     G +  +    GLEEL  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPW 242
            P PA  S I+A+PTIKI Q HL  SDS+CPVCK++FEL SEAK +PCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQESGHSWGGN---------GGSNSEDLNEREMTQRNLRRRHPFP 302
           LVQHN+CPVCR ELP   SG S   N           S+S   N RE       RR+PF 
Sbjct: 242 LVQHNSCPVCRQELP-SASGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 DFFAPRRIVTRFGS----GIHINGLSSDRDHD 304
            F+  R   +   S    G   N  +SD +H+
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHN 308

BLAST of IVF0009648 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.3e-30
Identity = 91/280 (32.50%), Postives = 129/280 (46.07%), Query Frame = 0

Query: 8   THWCYQCNH--SFWLDG-----EDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 67
           ++WCY C    S W D        V CPHC+GGF+E++ND      +             
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATE------------L 76

Query: 68  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFD--------VRRRSGSV 127
            +P   E       RR             +  R R +GR P+F+           R    
Sbjct: 77  TIPASTEVRSINNNRR-------------SVIRRRRSGRRPSFNPVIVLQGGAGEREEGE 136

Query: 128 PGQNLDFFNSFWSFHDHMSGSTFGNVTPEGRSS--QHTGLEELAAQLSLNEQ------RE 187
            G       +F  ++D  SGS      P+  S     +G E L  QLS  E       R 
Sbjct: 137 EGDAARDRRAFEFYYDDGSGSGL-RPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRS 196

Query: 188 PVPTPASHSCIEAMPTIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPW 247
             P PAS S IE++P ++I+  H+GS+++C VC E FE E+EA+ +PC H++H DCI+PW
Sbjct: 197 GNP-PASKSAIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPW 256

Query: 248 LVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMT 265
           L   N+CPVCR ELP + +  S      +N+E+ N   MT
Sbjct: 257 LSIRNSCPVCRFELPSEPNRRS------NNNEEDNAVGMT 263

BLAST of IVF0009648 vs. ExPASy Swiss-Prot
Match: O22283 (Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RHC2A PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 3.1e-29
Identity = 87/256 (33.98%), Postives = 126/256 (49.22%), Query Frame = 0

Query: 8   THWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDF--NPGIEEDLSTQVPPI 67
           ++WCY C+   W+  + + CP C+GGF+E + +  D T  + F      +    T+ PP 
Sbjct: 5   SYWCYSCSRFVWV-SDSISCPDCDGGFLELIQEPLDFTPSDSFTTTTTTQHRSPTRFPPP 64

Query: 68  FEA----MFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRR------SGSVPGQ 127
             +      ++    +P P         T      + R+PN  +  R      S  V  +
Sbjct: 65  SSSSSTPSASMHADNSPTP---------TIVTRTRSNRSPNPVIVLRGSAAAPSSDVVSE 124

Query: 128 NLDFFNSFWSFHDHMSGSTFGNVTPEGR----SSQHTGLEELAAQLSLNEQ---REPVPT 187
            LD  ++F  ++D  + S    + P        S    L +  +Q+ LN     R     
Sbjct: 125 GLD-RSAFQMYYDDGTDSGLRPLPPSMTEFLLGSGFDRLLDQISQIELNTNRNLRSCEHP 184

Query: 188 PASHSCIEAMPTIKINQMHLGSD--SHCPVCKEKFELESEAKALPCNHIYHTDCILPWLV 243
           PAS S IEA+P I+I+  HL SD  SHC VCKE F L+S A+ +PCNHIYH DCILPWL 
Sbjct: 185 PASKSAIEALPLIEIDPTHLLSDSQSHCAVCKENFVLKSSAREMPCNHIYHPDCILPWLA 244

BLAST of IVF0009648 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.9e-28
Identity = 92/282 (32.62%), Postives = 132/282 (46.81%), Query Frame = 0

Query: 10  WCYQCNHSFWL---DGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLSTQVPPIF 69
           +CYQCN +  +      D  CP CN GF+EE  D +     N FNP   +       P F
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLN-FNPNSSDSFFPMADP-F 81

Query: 70  EAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNSFWSF 129
             +  L+        FG   A  +   + M+   P+   + RS     Q+ D F+ F   
Sbjct: 82  STLLPLI--------FGSSAAAPS-GMDFMSLFGPSMQPQARSTQQNPQS-DAFDPFTFL 141

Query: 130 HDHM-----SGSTF-----------GNVTPE--GRSSQHTGLEELAAQLSLNEQREPVPT 189
            +H+     SG+ F           GN  P   G      GLE+L  QL+ N+       
Sbjct: 142 QNHLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTP 201

Query: 190 PASHSCIEAMPTIKINQMHLGSD-SHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQ 249
           PAS S I+A+PT+K+ +  L S+ + C VC ++FE  S+ K +PC H++H DC+LPWL  
Sbjct: 202 PASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLEL 261

Query: 250 HNTCPVCRLELPQQE---SGHSWGGNGGSNSEDLNEREMTQR 267
           HN+CPVCR ELP  +      S G  G  + +   E + T R
Sbjct: 262 HNSCPVCRFELPTDDPDYENRSQGSQGSGDGQGSVEGQQTPR 291

BLAST of IVF0009648 vs. ExPASy TrEMBL
Match: A0A1S3BQN3 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.5e-188
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR
Sbjct: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of IVF0009648 vs. ExPASy TrEMBL
Match: A0A5A7UIL6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00680 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.5e-188
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR
Sbjct: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of IVF0009648 vs. ExPASy TrEMBL
Match: A0A0A0LZS8 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.2e-179
Identity = 293/310 (94.52%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS GEDTHWC+QC+HSFWLDGED+VCPHC GGFVEELND+HDET+QNDFNPGIEEDLST
Sbjct: 59  MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 118

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 119 QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 178

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTF NVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 179 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 238

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFELESEAKALPCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 239 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 298

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGHSWGG+G +NSEDLNERE+TQRNLRRRHP PDFFAPRRIVTRF SGIHINGLSSDR
Sbjct: 299 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 358

Query: 301 DHDNEMGYPE 311
           DHDNEMGYPE
Sbjct: 359 DHDNEMGYPE 368

BLAST of IVF0009648 vs. ExPASy TrEMBL
Match: A0A6J1D235 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.6e-138
Identity = 233/314 (74.20%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGI-EEDLS 60
           MSS GEDTHWCYQCNHSFWL GED+VCP+CNGGF+EELND HD TIQND N G  EED S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF 120
           +QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRNPNFDVRRRS SVPGQNLDF
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMP 180
           FNSFWSFH+H+ G +F +V PEGRSSQ +GL+ L AQ+++NE+R+PVP PASHS IEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLG+DSHCPVCKEKFEL SEA+ +PCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQESGHSWGG---NGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGL 300
           QQ++ H+W G   +  SN E  N RE+TQ  L RR+P  DF+    ++ RF SGI  NG 
Sbjct: 241 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 300

Query: 301 SSDRDHDNEMGYPE 311
           S++  HDNEM YPE
Sbjct: 301 STNHYHDNEMSYPE 314

BLAST of IVF0009648 vs. ExPASy TrEMBL
Match: A0A6J1D364 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016591 PE=4 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 5.6e-138
Identity = 233/314 (74.20%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGI-EEDLS 60
           MSS GEDTHWCYQCNHSFWL GED+VCP+CNGGF+EELND HD TIQND N G  EED S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF 120
           +QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRNPNFDVRRRS SVPGQNLDF
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMP 180
           FNSFWSFH+H+ G +F +V PEGRSSQ +GL+ L AQ+++NE+R+PVP PASHS IEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLG+DSHCPVCKEKFEL SEA+ +PCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQESGHSWGG---NGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGL 300
           QQ++ H+W G   +  SN E  N RE+TQ  L RR+P  DF+    ++ RF SGI  NG 
Sbjct: 297 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 356

Query: 301 SSDRDHDNEMGYPE 311
           S++  HDNEM YPE
Sbjct: 357 STNHYHDNEMSYPE 370

BLAST of IVF0009648 vs. NCBI nr
Match: XP_008451039.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451041.1 PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0055692.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK09941.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 670 bits (1728), Expect = 1.19e-243
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR
Sbjct: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 310
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of IVF0009648 vs. NCBI nr
Match: XP_004144098.1 (E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_031736051.1 E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >KAE8653480.1 hypothetical protein Csa_007077 [Cucumis sativus])

HSP 1 Score: 639 bits (1649), Expect = 1.33e-231
Identity = 293/310 (94.52%), Postives = 303/310 (97.74%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS GEDTHWC+QC+HSFWLDGED+VCPHC GGFVEELND+HDET+QNDFNPGIEEDLST
Sbjct: 1   MSSFGEDTHWCHQCHHSFWLDGEDIVCPHCYGGFVEELNDEHDETVQNDFNPGIEEDLST 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
           QVPPIFEAMFALMGRR+PYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  QVPPIFEAMFALMGRRSPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFHDHMSGSTF NVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT
Sbjct: 121 NSFWSFHDHMSGSTFANVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFELESEAKALPCNHIYH DCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGTDSHCPVCKEKFELESEAKALPCNHIYHNDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGHSWGG+G +NSEDLNERE+TQRNLRRRHP PDFFAPRRIVTRF SGIHINGLSSDR
Sbjct: 241 QESGHSWGGSGDNNSEDLNEREITQRNLRRRHPIPDFFAPRRIVTRFSSGIHINGLSSDR 300

Query: 301 DHDNEMGYPE 310
           DHDNEMGYPE
Sbjct: 301 DHDNEMGYPE 310

BLAST of IVF0009648 vs. NCBI nr
Match: XP_038880729.1 (E3 ubiquitin-protein ligase RZF1-like [Benincasa hispida])

HSP 1 Score: 577 bits (1487), Expect = 5.47e-207
Identity = 271/310 (87.42%), Postives = 286/310 (92.26%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSSVGEDTHWCYQCNHSFWLDGEDVVCP+CNGGFVEE ND+HDET+Q+DFNPG E DLST
Sbjct: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPYCNGGFVEEFNDEHDETVQDDFNPGTE-DLST 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
            VPPIFEAMFALMGRR+PYPRFGLLEAVDT TRERM+GRNPNFDVRRRSGSVPGQNLDFF
Sbjct: 61  -VPPIFEAMFALMGRRSPYPRFGLLEAVDTLTRERMSGRNPNFDVRRRSGSVPGQNLDFF 120

Query: 121 NSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMPT 180
           NSFWSFH+HM G TF +VTPEGRSSQ TGLEELAAQLSL+E REPVPTP SHS I+AMPT
Sbjct: 121 NSFWSFHEHMPGPTFASVTPEGRSSQRTGLEELAAQLSLDEHREPVPTPTSHSSIQAMPT 180

Query: 181 IKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240
           IKINQMHLG+DSHCPVCKEKFELE EAK LPCNHIYHTDCILPWLVQHNTCPVCRLELPQ
Sbjct: 181 IKINQMHLGTDSHCPVCKEKFELECEAKVLPCNHIYHTDCILPWLVQHNTCPVCRLELPQ 240

Query: 241 QESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGLSSDR 300
           QESGH W GN GSNSE LNE  +TQRNLRR H FPDFFAPRRIVTRF SGIHINGLS+DR
Sbjct: 241 QESGHGWRGNSGSNSEGLNE--ITQRNLRR-HQFPDFFAPRRIVTRFSSGIHINGLSTDR 300

Query: 301 DHDNEMGYPE 310
           +HDN+M YPE
Sbjct: 301 EHDNDMSYPE 305

BLAST of IVF0009648 vs. NCBI nr
Match: XP_022147728.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia])

HSP 1 Score: 502 bits (1292), Expect = 4.67e-177
Identity = 233/314 (74.20%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEE-DLS 60
           MSS GEDTHWCYQCNHSFWL GED+VCP+CNGGF+EELND HD TIQND N G EE D S
Sbjct: 1   MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 60

Query: 61  TQVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF 120
           +QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRNPNFDVRRRS SVPGQNLDF
Sbjct: 61  SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 120

Query: 121 FNSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMP 180
           FNSFWSFH+H+ G +F +V PEGRSSQ +GL+ L AQ+++NE+R+PVP PASHS IEAMP
Sbjct: 121 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 180

Query: 181 TIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLG+DSHCPVCKEKFEL SEA+ +PCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 181 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 240

Query: 241 QQESGHSWGG---NGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGL 300
           QQ++ H+W G   +  SN E  N RE+TQ  L RR+P  DF+    ++ RF SGI  NG 
Sbjct: 241 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 300

Query: 301 SSDRDHDNEMGYPE 310
           S++  HDNEM YPE
Sbjct: 301 STNHYHDNEMSYPE 314

BLAST of IVF0009648 vs. NCBI nr
Match: XP_022147727.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia])

HSP 1 Score: 502 bits (1292), Expect = 3.51e-176
Identity = 233/314 (74.20%), Postives = 264/314 (84.08%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEE-DLS 60
           MSS GEDTHWCYQCNHSFWL GED+VCP+CNGGF+EELND HD TIQND N G EE D S
Sbjct: 57  MSSGGEDTHWCYQCNHSFWLGGEDIVCPYCNGGFIEELNDLHDGTIQNDTNLGTEEEDFS 116

Query: 61  TQVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDF 120
           +QVPPIFEAMFALMGRR+PYPRFGLLEAVD FTRERMAGRNPNFDVRRRS SVPGQNLDF
Sbjct: 117 SQVPPIFEAMFALMGRRSPYPRFGLLEAVDAFTRERMAGRNPNFDVRRRSSSVPGQNLDF 176

Query: 121 FNSFWSFHDHMSGSTFGNVTPEGRSSQHTGLEELAAQLSLNEQREPVPTPASHSCIEAMP 180
           FNSFWSFH+H+ G +F +V PEGRSSQ +GL+ L AQ+++NE+R+PVP PASHS IEAMP
Sbjct: 177 FNSFWSFHEHVPGPSFASVAPEGRSSQLSGLQALGAQITMNERRDPVPAPASHSSIEAMP 236

Query: 181 TIKINQMHLGSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPWLVQHNTCPVCRLELP 240
           TIKINQMHLG+DSHCPVCKEKFEL SEA+ +PCNHIYH+DCILPWLVQHNTCPVCRLELP
Sbjct: 237 TIKINQMHLGTDSHCPVCKEKFELGSEAREMPCNHIYHSDCILPWLVQHNTCPVCRLELP 296

Query: 241 QQESGHSWGG---NGGSNSEDLNEREMTQRNLRRRHPFPDFFAPRRIVTRFGSGIHINGL 300
           QQ++ H+W G   +  SN E  N RE+TQ  L RR+P  DF+    ++ RF SGI  NG 
Sbjct: 297 QQDTRHAWSGGSSSSSSNGEVSNGREITQLGLGRRNPLHDFWTTSNVLHRFSSGIRRNGT 356

Query: 301 SSDRDHDNEMGYPE 310
           S++  HDNEM YPE
Sbjct: 357 STNHYHDNEMSYPE 370

BLAST of IVF0009648 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 111/299 (37.12%), Postives = 147/299 (49.16%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS+  +THWC++C  + WL   D VC +C GGFVEE++       ++     +E D   
Sbjct: 1   MSSI-RNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRD-----VERD--- 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
                              P F L+EA   F R R+A R+ + ++  R GS   ++    
Sbjct: 61  -------------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNL 120

Query: 121 NSFWSF----------HDHMSGSTFGNVTPEGRSSQH----------TGLEELAAQLSLN 180
                F           D+ S   F N    G                GLEEL  QLS  
Sbjct: 121 APLLIFGGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 180

Query: 181 EQREPVPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTD 240
                 P PA  S I+A+PTIKI Q HL  SDSHCPVCK++FEL+SEAK +PC+HIYH+D
Sbjct: 181 THHRG-PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 240

Query: 241 CILPWLVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFF 279
           CI+PWLVQHN+CPVCR ELP +       G+  S     N     + N RRR+ F + +
Sbjct: 241 CIVPWLVQHNSCPVCRKELPSR-------GSSSSTQSSQNRSTNGRENSRRRNIFSNLW 263

BLAST of IVF0009648 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 111/299 (37.12%), Postives = 147/299 (49.16%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS+  +THWC++C  + WL   D VC +C GGFVEE++       ++     +E D   
Sbjct: 1   MSSI-RNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRD-----VERD--- 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
                              P F L+EA   F R R+A R+ + ++  R GS   ++    
Sbjct: 61  -------------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNL 120

Query: 121 NSFWSF----------HDHMSGSTFGNVTPEGRSSQH----------TGLEELAAQLSLN 180
                F           D+ S   F N    G                GLEEL  QLS  
Sbjct: 121 APLLIFGGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 180

Query: 181 EQREPVPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTD 240
                 P PA  S I+A+PTIKI Q HL  SDSHCPVCK++FEL+SEAK +PC+HIYH+D
Sbjct: 181 THHRG-PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 240

Query: 241 CILPWLVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFF 279
           CI+PWLVQHN+CPVCR ELP +       G+  S     N     + N RRR+ F + +
Sbjct: 241 CIVPWLVQHNSCPVCRKELPSR-------GSSSSTQSSQNRSTNGRENSRRRNIFSNLW 263

BLAST of IVF0009648 vs. TAIR 10
Match: AT3G56580.3 (RING/U-box superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.1e-44
Identity = 111/299 (37.12%), Postives = 147/299 (49.16%), Query Frame = 0

Query: 1   MSSVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLST 60
           MSS+  +THWC++C  + WL   D VC +C GGFVEE++       ++     +E D   
Sbjct: 1   MSSI-RNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRD-----VERD--- 60

Query: 61  QVPPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFF 120
                              P F L+EA   F R R+A R+ + ++  R GS   ++    
Sbjct: 61  -------------------PTFDLMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNL 120

Query: 121 NSFWSF----------HDHMSGSTFGNVTPEGRSSQH----------TGLEELAAQLSLN 180
                F           D+ S   F N    G                GLEEL  QLS  
Sbjct: 121 APLLIFGGQAPFRLAGGDNSSVEAFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSG 180

Query: 181 EQREPVPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTD 240
                 P PA  S I+A+PTIKI Q HL  SDSHCPVCK++FEL+SEAK +PC+HIYH+D
Sbjct: 181 THHRG-PPPAPKSSIDALPTIKITQKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSD 240

Query: 241 CILPWLVQHNTCPVCRLELPQQESGHSWGGNGGSNSEDLNEREMTQRNLRRRHPFPDFF 279
           CI+PWLVQHN+CPVCR ELP +       G+  S     N     + N RRR+ F + +
Sbjct: 241 CIVPWLVQHNSCPVCRKELPSR-------GSSSSTQSSQNRSTNGRENSRRRNIFSNLW 263

BLAST of IVF0009648 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 173.7 bits (439), Expect = 2.3e-43
Identity = 122/332 (36.75%), Postives = 163/332 (49.10%), Query Frame = 0

Query: 3   SVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLSTQV 62
           S   +THWC++C  +  L G++ VC +C GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHM--------------SGSTFGNVTPEGRSSQH---TGLEELAAQLSLNEQREP 182
              F   +              +G + G     G +  +    GLEEL  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPW 242
            P PA  S I+A+PTIKI Q HL  SDS+CPVCK++FEL SEAK +PCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQESGHSWGGN---------GGSNSEDLNEREMTQRNLRRRHPFP 302
           LVQHN+CPVCR ELP   SG S   N           S+S   N RE       RR+PF 
Sbjct: 242 LVQHNSCPVCRQELP-SASGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 DFFAPRRIVTRFGS----GIHINGLSSDRDHD 304
            F+  R   +   S    G   N  +SD +H+
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHN 308

BLAST of IVF0009648 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 173.7 bits (439), Expect = 2.3e-43
Identity = 122/332 (36.75%), Postives = 163/332 (49.10%), Query Frame = 0

Query: 3   SVGEDTHWCYQCNHSFWLDGEDVVCPHCNGGFVEELNDDHDETIQNDFNPGIEEDLSTQV 62
           S   +THWC++C  +  L G++ VC +C GGFVEEL                  D++   
Sbjct: 2   SSSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEEL------------------DMAQAS 61

Query: 63  PPIFEAMFALMGRRNPYPRFGLLEAVDTFTRERMAGRNPNFDVRRRSGSVPGQNLDFFNS 122
           P  F+   +  G       F L++A   F R R+A R+ + ++R R+ S   +N      
Sbjct: 62  P--FDMFRSHRGVVERDQTFDLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAP 121

Query: 123 FWSFHDHM--------------SGSTFGNVTPEGRSSQH---TGLEELAAQLSLNEQREP 182
              F   +              +G + G     G +  +    GLEEL  QLS    R  
Sbjct: 122 LLIFGGQVPYRLTGDNAVEALFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRG 181

Query: 183 VPTPASHSCIEAMPTIKINQMHL-GSDSHCPVCKEKFELESEAKALPCNHIYHTDCILPW 242
            P PA  S I+A+PTIKI Q HL  SDS+CPVCK++FEL SEAK +PCNHIYH+DCI+PW
Sbjct: 182 -PPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPW 241

Query: 243 LVQHNTCPVCRLELPQQESGHSWGGN---------GGSNSEDLNEREMTQRNLRRRHPFP 302
           LVQHN+CPVCR ELP   SG S   N           S+S   N RE       RR+PF 
Sbjct: 242 LVQHNSCPVCRQELP-SASGPSSSQNRTTPTRNYRSSSSSSSSNSRE---NGNERRNPFS 301

Query: 303 DFFAPRRIVTRFGS----GIHINGLSSDRDHD 304
            F+  R   +   S    G   N  +SD +H+
Sbjct: 302 SFWPFRSSGSSSSSTQNRGGTRNSDTSDENHN 308

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AK42.9e-4337.12E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
O221973.2e-4236.75Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q940T56.3e-3032.50E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ... [more]
O222833.1e-2933.98Probable E3 ubiquitin-protein ligase RHC2A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q8LPN75.9e-2832.62E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
Match NameE-valueIdentityDescription
A0A1S3BQN31.5e-188100.00RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103492446 PE=4 ... [more]
A0A5A7UIL61.5e-188100.00RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A0A0LZS82.2e-17994.52RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G600760 PE... [more]
A0A6J1D2355.6e-13874.20RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
A0A6J1D3645.6e-13874.20RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101659... [more]
Match NameE-valueIdentityDescription
XP_008451039.11.19e-243100.00PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >XP_008451040.1... [more]
XP_004144098.11.33e-23194.52E3 ubiquitin-protein ligase RZF1 [Cucumis sativus] >XP_011660068.1 E3 ubiquitin-... [more]
XP_038880729.15.47e-20787.42E3 ubiquitin-protein ligase RZF1-like [Benincasa hispida][more]
XP_022147728.14.67e-17774.20probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Momordica charantia][more]
XP_022147727.13.51e-17674.20probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G56580.22.1e-4437.12RING/U-box superfamily protein [more]
AT3G56580.12.1e-4437.12RING/U-box superfamily protein [more]
AT3G56580.32.1e-4437.12RING/U-box superfamily protein [more]
AT2G40830.22.3e-4336.75RING-H2 finger C1A [more]
AT2G40830.12.3e-4336.75RING-H2 finger C1A [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 194..234
e-value: 1.4E-7
score: 41.1
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 193..235
e-value: 2.0E-11
score: 44.1
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 194..235
score: 12.577796
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 156..241
e-value: 2.2E-19
score: 70.9
IPR039525E3 ubiquitin-protein ligase RNF126-like, zinc-ribbonPFAMPF14369zinc_ribbon_9coord: 8..38
e-value: 7.9E-11
score: 42.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..271
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 243..258
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 3..146
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 149..309
NoneNo IPR availablePANTHERPTHR15710:SF86E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATEDcoord: 149..309
NoneNo IPR availablePANTHERPTHR15710:SF86E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATEDcoord: 3..146
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 194..235
e-value: 3.07074E-22
score: 85.4472
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 174..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009648.2IVF0009648.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0061630 ubiquitin protein ligase activity