IVF0009618 (gene) Melon (IVF77) v1

Overview
NameIVF0009618
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionL-ascorbate oxidase
Locationchr02: 3655854 .. 3660197 (+)
RNA-Seq ExpressionIVF0009618
SyntenyIVF0009618
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGTGTCGTTATTCATTGGCATGGAATTCTGCAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGGTGAGAAGGATTTAAAATGTCAATATCGATGGAAGTGTGTCATAGTTATATTTATGATAAAATCTATTCATTTGTTGATTTTTTTTCTTTTTGTAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCCCCAGGTAGTAAATTATGTTCATATTGACAACTAATATAGGTTTCTTAGTTAAATTAAAAAGACAAAATTATTTCTCATTTTTTTTCAAAATTTGACATATATTTGAAACATTAGTAGAAAGTAGATAATAAACTAGAGAAACTTAACGTGAAAAAAAGTATTTTTAAGCTTAACAATTATACAAAAAAAATTGTATATTTTTGGGAACTATTTGATCTTTTTGTTTTTGTTTTGTCAAAGTAGGTTGCTTTCGTGATCCTCATTTAACAACATACGAAGAAAAGTGGTTTTTTGAAAACTATTACTTTTGAGTTTCCAAAATTTAACTTGGTTTATGAAAACTTATGACAGAAATGTATAACAAAGCAAGAATTTAGAAGTAGAAGAGTTATTTATAGGCTTAACTTTCAAAGATCAAACATTTAATTCATTCTTTTCTTTTTTAGAAAAATTGAAAACTAAGTTTATTTGATTCCTAAAGTTTCAAAACGAAAAATTTAATATACCTGAATGTTTGAGAAACAATGACTATTTAGTCAGCGGGTTGATTAATGGTAAAATGAGTTTAATTATGCTTCATTTACTTCTTTTCACTTTGACATTTTTTATTTACAAACCCATCTCCTTCCTTCTTCTCCATCGCCTTCTTCAACCTTACCTCCTCCTCAAGGTCTCTTTCAATAATCCTCAAACCATTGCTCAGATTTTCACAAAATCTATTTGAACTTTAATTTCGGCTTCATATCTTTACTTATGTTCCGATTCATTAGACGACGCTAGCACAAACTTAAAAAAGAGTTATTGTATCCTCTTACCAACACTTCATAAACATAGAAGAAGAGAGCAAAAAAGAGAAAAGCCAACATCATTTAGAATCAAAACATTCCTTTTAATACTGAAGTAAAGGAGATTGCCTAATCAGCTAGCAAACTCATTTTTTAACATTTTTCACACCATTTTTTCCTTCGTTAACATTTAAAATCATTTCTTAAACTTTATAGAATAAAGTTTTCATTTCAAAATTTCGAGACCAAACATACGTCAAGTTTGTCATTTTAATTTTTAGTCAATTTTTGAATTGAGAAAATATCAACTAAAATTTTAGCTCAAATTCAAAGGACAATAATTTTATCTCAAACTAGAAATATCATCGCAGAATTTGATAAAATATTCGAACTTTGTAATAAAATTGATTTTTGTCATGAAGTATAACCATTTTGTTTATTTATTTATTTATTTATTATATTATTCTTAAAAAAACCCATAAATAATAGGAAAACCTAGAGTTTAATAATCCAATTATTAATAATCCAATCATGTTAGGCCTACCCATTTCTTAAGGATGGGCAGGTGTCTCCGAATTTTGAATTTTATAATAATTTGTAGGGAAATTCCATGCGACATACTTTTCAAAATTTATATAAATCACATAATTTAAATCTAATTGAAAAAAACATTAGAGATAACGGATGGGCCACCAAAATTACTCGCATTCGGAGATAGATAATGATATTTTGTTATATATTTGTATATATTTCAAATTTTGGGATTTAAAATAATTATCCTTTAAACTAACTAATCTTATATATAATTAGTTGTCTCATTCATATTTATCGATAACTATAATTGATAGAACTTAAAATTTTAATTTTTTTTACAATTTTGCTCTAATAAACAAAGCTTAGATTATATTTGACCGTATGAGAAAGTAGATAATAATTAAAAATTGGTTGATATTTTTTTCTTGAAATTTGTCATTAACGTTTGTTTGATTTAGGTAAAATACTTGAGATGTTTAAGTATAATGTCTTTTTTTCTTTTTGTCTCTTGTTCTTGAAGACCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGGCATTGCGGTAACACAACCTCTCACTTAGTTCTTTTTACAACAATTACAGAAATAAAAAATTGAATCCAATATTCTATCTCAAGAATGAAAAGTTATATCTGTTAGCTCTATGTTTATCCTTGACTTTTGAGATGTATGTCTATTTAAGTAAATATTCGAATTGTTCATTTATCAAATGAGGCTTAAAAATATAATACATTGTGAATTTAGATTTTCAAGTGATTGGACTTAGGTTTAGAAGGAAAAAAATATTATTTTCAAATATAACAAAATAAACCAAAATAACTGTATGTTTAGGGTATTTATAAATATTTTTAAAAAATTTTGTCATTTAAAATAATTTTTCAAAAATAATAACCGACATGCATATATAATTTTTTTGGAAAATAAATCATACCCCAAATTATATATTCGACCATAATCTCAAAATTTTATGAAATAAATTTTGGGTTTTTTTTTTTTTTTCTAACCATCTTAAAATAAAAAGTTTTAATACTTTTTCTTTTCTTTTCCAGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCGACCGCAGATGAGACGAAATTGAATCTGCAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTGAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTAATAATTTGA

mRNA sequence

ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGTGTCGTTATTCATTGGCATGGAATTCTGCAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCCCCAGACCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGGCATTGCGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCGACCGCAGATGAGACGAAATTGAATCTGCAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTGAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTAATAATTTGA

Coding sequence (CDS)

ATGGCAAAAGTTGCAAATCAGCCATTGTTTCCTTTGCCTTTTCTAGTCTTCTTTGTTTTGTCAATCATATTTGGTTTTGGAATAACTCTCTCTAAGGCTGTTTCCCAAACCAGATACTACAAATTCGAAGTTGACTACATGTTTTGGTCCCCGGATTGTGTTGAAAACATCGTCTTGGGAATCAATGGTACATTTCCCGGTCCACCGATTAGAGCCAATGTTAATGACACCGTGGTTGTGGAGGTGATCAACAATCTCTCCACCGAAGGTGTCGTTATTCATTGGCATGGAATTCTGCAACGAGGAACTCCCTGGGCCGATGGCACTGCTTCCATCTCTCAGTGTCCCATTAATCCAGGAGAAAACTTCACTTACGAGTTCAAAGTGGATAAGCCTGGGACATATTTCTATCATGGTCATTTTGGGATGCAGAGAGCGGCTGGGTTGTATGGATCCTTAATAGTGGATTTAGAGGAAGGAAAGAAAGAGCCCTTCCAATATGACGAAGAGATCAACTTATTACTTAGTGATTGGTGGCATACAAGCATTCAGGAGCAAGAAGTTGGCCTCAAGTCAAATCCAATGCGCTGGATTGGAGAGCCCCAGACCATACTCATAAATGGGACAGGGCAATTTAATTGTACAAGTTTAGATAAATACAACACGAGTTCGGAGCATTGTGAGTGGAAAGATCAATGTGCACCATTTGTCCTCAAAGTCGAACCCAATAAGACTTATAGGATAAGAATTGCAAGTACCACTTCATTATCTGCTCTCAATTTTGGCATTGCGAATCACACAATGTTGGTGGTAGAAGCCGACGGTAACTACGTTGACCCATTTGAAACCTCCGATATCGACATTTATTCCGGCGAGTCCTACTCTGTTCTCGTCACCACTAACCAAGCCACAACCCAAAACTACTGGGCTTCCATAGGCGTCCGTGGTCGGAAGCCTAAAACATCGCCGGCACTCCTCCTCTTCAACTACCTTCCCAACTCCGTCGCCAAATTACCCATTTCTCCACCGCCAGAAACCCCCAAATGGGATGATTACGATCGAAGTAAAAGCTTCACAAACAGAGTCTTAGCCTCTGCCATGAACACAACAAAACCACCACCAATCTTCACTCGCCGGATCTCCCTCCTCAACACCCAAAATCGTATTAACGGCAAATTCACAAAATGGGCAATCAACAACATATCATTAACCCTCCCCTCAACCCCGTACCTCGGCGCCATTAAATTCGGTCTCAACTCCACCTTCAACCAGACTCCGCCACCAGAAACATTCCCAAAGAACTACAACATCAAAATCCCACCGCCGAACCCGAACACGAATACCGGAAACGGCGTTTATCGGTTCCGATCGGGGGAAATAGTGGACGTGATTCTACAAAACGCCAATGCGTTAACAAACAATACAAGTGAAATTCACCCTTGGCATTTACACGGCCATGATTTTTGGGTTTTGGGGTTTGGAGAGGGGAAATTTTCGACCGCAGATGAGACGAAATTGAATCTGCAAAACCCACCGTTGAAGAACACGGTAGTGATTTTCCCATTTGGGTGGACGGCGATTCGATTTGTGGCGGATAATCCCGGCGTTTGGATATTCCATTGCCACATTGAACCTCATTTGGATATGGGAATGGGGGTTATCTTCGCCGAAGCAGTTGAAAGGGTGAGGGAAATTCCGCCGTATGCTTTGGCTTGCGGCCGCACTGCCACACATAACGTTGTTCCAATAATCCCACAACCCAAACCACCCAGAGGACCCGGACCAGTTATCCCCTACTTCATGCTTTGGGTAATTGGGTTAGTTATAATTGTGTGTGGGATTGGACTGTTTGGTGTCTGTCTTTATCGGATTGGATTGCGTCGGGGTTATACTTTAATAATTTGA

Protein sequence

MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKVEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRIGLRRGYTLII
Homology
BLAST of IVF0009618 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 901.7 bits (2329), Expect = 4.6e-261
Identity = 430/589 (73.01%), Postives = 480/589 (81.49%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKA-VSQTRYYKFEVDYMFWSPDCVENIVL 60
           MAKVA++P FP PFL F VLSIIFGFGITLS+A   + ++YK++V+YMFWSPDCVENIV+
Sbjct: 1   MAKVADKPFFPKPFLSFLVLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVM 60

Query: 61  GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINP 120
           GING FPGP IRAN  D VVVE+ N L TEGVVIHWHGILQRGTPWADGTASISQC INP
Sbjct: 61  GINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINP 120

Query: 121 GENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWW 180
           GE FTY F VDK GTYFYHGH GMQR+AGLYGSLIVD  EG+ EPF YDEEINLLLSDWW
Sbjct: 121 GETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWW 180

Query: 181 HTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFV 240
           H S+ +QEVGL S PMRWIGEPQ+ILING GQF+C+   KYN   + CE   K++CAPF+
Sbjct: 181 HQSVHKQEVGLSSKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFI 240

Query: 241 LKVEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVL 300
           L V+P KTYRIRIASTT+L++LNF I NH +LVVEADGNYV PF TSDIDIYSGESYSVL
Sbjct: 241 LHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVL 300

Query: 301 VTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKS 360
           +TT+Q   +NYW SIGVR R PKT P L L NYLPNS +KLPISPPPETP W+D+DRSK+
Sbjct: 301 ITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKN 360

Query: 361 FTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFG 420
           FT R+ A AM + KPP  + RR+ LLNTQNRING F KWAINN+SL LP TPYL A+K  
Sbjct: 361 FTFRIFA-AMGSPKPPVRYNRRLFLLNTQNRING-FMKWAINNVSLALPPTPYLAAMKMR 420

Query: 421 LNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSE 480
           LN+ FNQ PPPETFP NY+I  PPPNP T TGNGVY+F  GE VDVILQNAN L  N SE
Sbjct: 421 LNTAFNQNPPPETFPLNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSE 480

Query: 481 IHPWHLHGHDFWVLGFGEGKF-STADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLG+GEGKF +  DE KLNL+NPPL+NTVVIFP+GWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIP 586
           W FHCHIEPHL MGMGV+FAE V  V  IPP ALACG TA     P +P
Sbjct: 541 WAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNYPRLP 587

BLAST of IVF0009618 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 847.8 bits (2189), Expect = 7.9e-245
Identity = 399/565 (70.62%), Postives = 456/565 (80.71%), Query Frame = 0

Query: 14  FLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRAN 73
           FL+ F   +I  FGI  S   SQ R+YK+EV+YMFW+PDC ENIV+GING FPGP IRAN
Sbjct: 12  FLILFFFGLILAFGI--SSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRAN 71

Query: 74  VNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPG 133
             DTVVVE+IN L TEGVVIHWHGILQRGTPWADGTASISQC INPGE F Y F VD PG
Sbjct: 72  AGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPG 131

Query: 134 TYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSN 193
           T+FYHGH GMQR+AGLYGSLIVD  +GKKEPF YD EINLLLSDWWH SI +QEVGL S 
Sbjct: 132 TFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSK 191

Query: 194 PMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 253
           P+RWIGEPQTIL+NG GQF+C+   KY+++ E C+ K  + CAP++  V P KTYRIRIA
Sbjct: 192 PIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIA 251

Query: 254 STTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 313
           STT+L+ALNF I NH +LVVEADGNYV PF TSDIDIYSGESYSVL+TT+Q  ++NYW S
Sbjct: 252 STTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVS 311

Query: 314 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 373
           +G RGR P T P L L NYLPNSV+KLP SPPPETP WDD+DRSK+FT R+ A AM + K
Sbjct: 312 VGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITA-AMGSPK 371

Query: 374 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 433
           PP    RRI LLNTQN ING + KWAIN++SL LP TPYLGA+KF L   F+Q PPPE F
Sbjct: 372 PPVKSNRRIFLLNTQNVING-YVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVF 431

Query: 434 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 493
           P++Y+I  PP N  T  GNGVY+F+ GEIVDVILQNAN +  N SEIHPWHLHGHDFWVL
Sbjct: 432 PEDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVL 491

Query: 494 GFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGM 553
           G+G+GKF+  +E+ LNL+NPPL+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MGM
Sbjct: 492 GYGDGKFTAEEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGM 551

Query: 554 GVIFAEAVERVREIPPYALACGRTA 577
           GV+FAE VE+V  IP  ALACG TA
Sbjct: 552 GVVFAEGVEKVGRIPTKALACGGTA 572

BLAST of IVF0009618 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 2.0e-240
Identity = 389/558 (69.71%), Postives = 449/558 (80.47%), Query Frame = 0

Query: 35  SQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIH 94
           SQ R+YK+EV+YMFW+P+C ENIV+GING FPGP IRAN  D+VVVE+ N L TEGVVIH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 95  WHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLI 154
           WHGILQRGTPWADGTASISQC INPGE F Y F VD PGT+FYHGH GMQR+AGLYGSLI
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 155 VDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNC 214
           VD  +GKKEPF YD EINLLLSDWWH SI +QEVGL S P+RWIGEPQTIL+NG GQF+C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 215 TSLDKYNTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLSALNFGIANHTMLVVE 274
           +   KY+++ E C+ K  + CAP++  V P KTYRIRIASTT+L+ALNF I NH +LVVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 275 ADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLP 334
           ADGNYV PF TSDIDIYSGESYSVL+TT+Q  ++NYW S+G R R P T P L L NYLP
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 335 NSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGK 394
           NSV+KLP SPPP+TP WDD+DRSK+FT R+ A AM + KPP  F RRI LLNTQN ING 
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITA-AMGSPKPPVKFNRRIFLLNTQNVING- 360

Query: 395 FTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGV 454
           + KWAIN++SL LP TPYLGA+K+ L   F+Q PPPE FP++Y+I  PP N  T  GNGV
Sbjct: 361 YVKWAINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFPEDYDIDTPPTNEKTRIGNGV 420

Query: 455 YRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSTADETKLNLQNPP 514
           Y+F+ GE+VDVILQNAN +  N SE HPWHLHGHDFWVLG+G+GKFS  +E+ LNL+NPP
Sbjct: 421 YQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSAEEESSLNLKNPP 480

Query: 515 LKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALAC 574
           L+NTVVIFP+GWTAIRFVADNPGVW FHCHIEPHL MGMGV+FAE VE+V  IP  ALAC
Sbjct: 481 LRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALAC 540

Query: 575 GRTATHNVVPIIPQPKPP 591
           G TA      +I  PK P
Sbjct: 541 GGTAK----SLINNPKNP 552

BLAST of IVF0009618 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 5.9e-216
Identity = 355/569 (62.39%), Postives = 435/569 (76.45%), Query Frame = 0

Query: 12  LPFLVFFVLSIIFGFGITLSKAV--SQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPP 71
           L FL FF+L +I    ++ S++V  ++TR++K++V+Y+ WSPD  E++V+GING FPGP 
Sbjct: 4   LGFLFFFLLPLIL-LELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPT 63

Query: 72  IRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKV 131
           IRA   DTV V + N L TEGVVIHWHGI Q GTPWADGTA+ISQC INPGE F Y FKV
Sbjct: 64  IRAKAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKV 123

Query: 132 DKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVG 191
           DK GTYFYHGH+GMQR+AGLYGSLIV++ EG+KEPF YD E NLLLSDWWH    EQEV 
Sbjct: 124 DKAGTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVD 183

Query: 192 LKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSS-EHCEWK--DQCAPFVLKVEPNKTY 251
           L SNP+RWIGEPQT+L+NG GQ+NC+   +++      C+ +  +Q AP +L+V PNK Y
Sbjct: 184 LSSNPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIY 243

Query: 252 RIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQ 311
           R+R+ASTT+L +L+  I  H M+VVEADGNYV PF   D+DIYSGESYSVL  T+Q  T+
Sbjct: 244 RLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTK 303

Query: 312 NYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASA 371
           NYW SI VRGR+PKT   L L NYLPNS +K P  PPP  P W+DY+ SKSF+N++ A  
Sbjct: 304 NYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFA-L 363

Query: 372 MNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTP 431
           M + KPPP   RRI LLNTQN+I+G +TKWAINN+SL LP+  YLG+I++G+N+ F+  P
Sbjct: 364 MGSPKPPPQNHRRIILLNTQNKIDG-YTKWAINNVSLVLPTQLYLGSIRYGINA-FDTKP 423

Query: 432 PPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGH 491
           PP+ FPK+Y++    PN N+  GNGVY  +    +D+ILQNANAL  + SEIHPWHLHGH
Sbjct: 424 PPDNFPKDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLHGH 483

Query: 492 DFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPH 551
           DFWVLG+GEGKFS  D  K NL+NPPL+NT VIFPFGWTA+RFV DNPGVW FHCHIEPH
Sbjct: 484 DFWVLGYGEGKFSEKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHIEPH 543

Query: 552 LDMGMGVIFAEAVERVREIPPYALACGRT 576
           L MGMGVIFAE V  V++IP  ALACG T
Sbjct: 544 LHMGMGVIFAEGVHLVKKIPKEALACGLT 568

BLAST of IVF0009618 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 4.5e-208
Identity = 335/557 (60.14%), Postives = 416/557 (74.69%), Query Frame = 0

Query: 23  IFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVNDTVVVEV 82
           I    I    A +  R Y +EV+Y F  PDC E +V+ +NG FPGP I A   DT+VV +
Sbjct: 6   IVAVAILAHTASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHL 65

Query: 83  INNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGTYFYHGHFG 142
            N L+TEG+VIHWHGI Q G+PWADG A ++QC I+PGE FTY F VDKPGT+FYHGH+G
Sbjct: 66  TNKLATEGLVIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYG 125

Query: 143 MQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPMRWIGEPQ 202
           MQR+AGLYGSLI+D+ +GKKEP +YD E NLLLSDWWH  +  QE+GL S PMRWIGE Q
Sbjct: 126 MQRSAGLYGSLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSSRPMRWIGEAQ 185

Query: 203 TILINGTGQFNCTSLDKY-NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLSAL 262
           +ILING GQFNC+   ++ +TS   C +K  DQCAP  L VEPNKTYRIR+AS+T+L++L
Sbjct: 186 SILINGRGQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASL 245

Query: 263 NFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGRKP 322
           NF +  H ++VVEADGNY+ PF T DIDIYSGE+YSVL+TT+Q  +QNY+ + GVRGRKP
Sbjct: 246 NFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKP 305

Query: 323 KTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFTRR 382
            T PAL + NY+    ++LP SPPPETP+W+D+DRSK+F+ ++ A AM +  PP  F  R
Sbjct: 306 NTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFA-AMGSPSPPETFDER 365

Query: 383 ISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNIKI 442
           + LLNTQN I G FTKWAINN+SL +P TPYLG++K+ L + FN++ PP+ +P +Y+I  
Sbjct: 366 LILLNTQNLIEG-FTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPVDYDIMT 425

Query: 443 PPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGKFS 502
           PP N N   GN    F     VDVILQNAN L  N SEIHPWHLHGHDFWVLG+GEGKF 
Sbjct: 426 PPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFK 485

Query: 503 T-ADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFAEA 562
              DE   NL+NPPL+NTV ++P+GWTA+RFV DNPGVW FHCHIEPHL MGMGV+FAE 
Sbjct: 486 PGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEG 545

Query: 563 VERVREIPPYALACGRT 576
           + R+ ++P  AL CG T
Sbjct: 546 LNRIGKVPDEALGCGLT 560

BLAST of IVF0009618 vs. ExPASy TrEMBL
Match: A0A1S3BQ56 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492477 PE=3 SV=1)

HSP 1 Score: 1312.4 bits (3395), Expect = 0.0e+00
Identity = 626/631 (99.21%), Postives = 627/631 (99.37%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGE Q ILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNF IANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFGEGKFS ADETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 632
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631

BLAST of IVF0009618 vs. ExPASy TrEMBL
Match: Q9ZR59 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=ao3 PE=3 SV=1)

HSP 1 Score: 1307.7 bits (3383), Expect = 0.0e+00
Identity = 624/631 (98.89%), Postives = 626/631 (99.21%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGE Q ILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNF IANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFG+ KFS ADETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 632
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631

BLAST of IVF0009618 vs. ExPASy TrEMBL
Match: A0A0A0M2J6 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G602530 PE=3 SV=1)

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 568/637 (89.17%), Postives = 592/637 (92.94%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GEPQTILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFGIA-----------NHTMLVVEADGNYVDPFETSDID 300
           KVEPNKTYRIRIASTTSL+ALNF I+           NHTMLVVEADGNYV  F TSDID
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISKKFHHFSFLFQNHTMLVVEADGNYVQQFVTSDID 300

Query: 301 IYSGESYSVLVTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETP 360
           IYSGESYSVLVTTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE P
Sbjct: 301 IYSGESYSVLVTTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIP 360

Query: 361 KWDDYDRSKSFTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPS 420
           KW+DYDRSK+FT RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPS
Sbjct: 361 KWNDYDRSKNFTYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPS 420

Query: 421 TPYLGAIKFGLNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQN 480
           TPYLGAIKFGLNSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQN
Sbjct: 421 TPYLGAIKFGLNSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQN 480

Query: 481 ANALTNNTSEIHPWHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAI 540
           ANAL+NNTSEIHPWHLHGHDFWVLG+GEGKFS  DETKLNL+NPPLKNTVVIFPFGWTAI
Sbjct: 481 ANALSNNTSEIHPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAI 540

Query: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQP 600
           RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P
Sbjct: 541 RFVADNPGVWIFHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRP 600

Query: 601 KPPRGPGPVIPYFMLWVIGLVIIVCGIGLFGVCLYRI 625
            P   PG   PY+++W IGLVI +C IGL  VCLYRI
Sbjct: 601 NP---PGLDNPYYIIW-IGLVIFLCVIGLCYVCLYRI 633

BLAST of IVF0009618 vs. ExPASy TrEMBL
Match: Q9M5B5 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 1.1e-268
Identity = 441/578 (76.30%), Postives = 499/578 (86.33%), Query Frame = 0

Query: 3   KVANQPLFPLPF-LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGI 62
           KVANQPLFP+PF  +FF+ SI FGFGITLS+A SQTR++K+EV+YMF SPDC EN+V+GI
Sbjct: 4   KVANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGE 122
           N  FPGP IRANV DTVVVE+IN LSTE VVIHWHGILQ+GTPWADGTASISQC INPGE
Sbjct: 64  NHQFPGPTIRANVGDTVVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 NFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHT 182
           +FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH 
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFVLK 242
           S+ +QEVGL S+PMRWIGEPQ+ILING GQ NC+   KY  + E C+    ++CAPF+LK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEECAPFILK 243

Query: 243 VEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVT 302
           V+PNKTYRIRI STTSLSALNF I NH +LVVEADGNYV PF TSDIDIYSGESYSVL+ 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFT 362
           T+Q  +QNYW S+GVRGR+PKT P L L NYLPNS +KLPISPPPETPKW+DYDRSK+FT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 NRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLN 422
            R+LA AM + KPP   TRRI LLNTQN++NG +TKWAINN+SLTLPSTPYLGAIK G+N
Sbjct: 364 YRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIN 423

Query: 423 STFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 482
            TF+Q PPPET+P+NY+I  PPPN NT TGNGVYRF+ GEIVDV+LQNAN L +  SEIH
Sbjct: 424 DTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIH 483

Query: 483 PWHLHGHDFWVLGFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWI 542
           PWHLHGHDFWVLG+GEGKFS A DETKLNL++PPL+NTVVIFPFGWT +RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWA 543

Query: 543 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 577
           FHCHIEPHL MGMGV+FAE V++V  IP +A+ACG TA
Sbjct: 544 FHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of IVF0009618 vs. ExPASy TrEMBL
Match: A0A5D3CF11 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G00020 PE=3 SV=1)

HSP 1 Score: 931.0 bits (2405), Expect = 2.6e-267
Identity = 439/578 (75.95%), Postives = 497/578 (85.99%), Query Frame = 0

Query: 3   KVANQPLFPLPF-LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGI 62
           K ANQPLFP+PF  +FF+ SI FGFGITLS+A SQTR++K+EV+YMF SPDC EN+V+GI
Sbjct: 4   KDANQPLFPMPFPAIFFIFSIFFGFGITLSQAASQTRHFKWEVEYMFRSPDCKENLVMGI 63

Query: 63  NGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGE 122
           N  FPGP IRANV DT+VVE+IN LSTE VVIHWHGILQ+GTPWADGTASISQC INPGE
Sbjct: 64  NHQFPGPTIRANVGDTIVVELINKLSTEDVVIHWHGILQKGTPWADGTASISQCAINPGE 123

Query: 123 NFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHT 182
           +FTY+F VDKPGTYFYHGH GMQRAAGLYGSLIVDLEEGKKEPF YDEEINLLLSDWWH 
Sbjct: 124 SFTYQFVVDKPGTYFYHGHLGMQRAAGLYGSLIVDLEEGKKEPFHYDEEINLLLSDWWHE 183

Query: 183 SIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCE--WKDQCAPFVLK 242
           S+ +QEVGL S+PMRWIGEPQ+ILING GQ NC+   KY  + E C+    + CAPF+LK
Sbjct: 184 SVLKQEVGLSSSPMRWIGEPQSILINGRGQSNCSIGAKYMKNMEQCKLSGSEGCAPFILK 243

Query: 243 VEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVT 302
           V+PNKTYRIRI STTSLSALNF I NH +LVVEADGNYV PF TSDIDIYSGESYSVL+ 
Sbjct: 244 VKPNKTYRIRIISTTSLSALNFAIGNHKLLVVEADGNYVQPFLTSDIDIYSGESYSVLIN 303

Query: 303 TNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFT 362
           T+Q  +QNYW S+GVRGR+PKT P L L NYLPNS +KLPISPPPETPKW+DYDRSK+FT
Sbjct: 304 TDQNPSQNYWVSLGVRGREPKTPPGLTLLNYLPNSFSKLPISPPPETPKWNDYDRSKNFT 363

Query: 363 NRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLN 422
            R+LA AM + KPP   TRRI LLNTQN++NG +TKWAINN+SLTLPSTPYLGAIK G+N
Sbjct: 364 YRILA-AMGSPKPPVKSTRRIFLLNTQNKMNGGYTKWAINNVSLTLPSTPYLGAIKIGIN 423

Query: 423 STFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 482
            TF+Q PPPET+P+NY+I  PPPN NT TGNGVYRF+ GEIVDV+LQNAN L +  SEIH
Sbjct: 424 DTFDQNPPPETYPENYDIHKPPPNGNTITGNGVYRFQLGEIVDVVLQNANTLNDKHSEIH 483

Query: 483 PWHLHGHDFWVLGFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWI 542
           PWHLHGHDFWVLG+GEGKFS A DETKLNL++PPL+NTVVIFPFGWT +RFVADNPGVW 
Sbjct: 484 PWHLHGHDFWVLGYGEGKFSAAVDETKLNLKDPPLRNTVVIFPFGWTVLRFVADNPGVWA 543

Query: 543 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 577
           FHCHIEPHL MGMGV+FAE V++V  IP +A+ACG TA
Sbjct: 544 FHCHIEPHLHMGMGVVFAEGVDKVGRIPTHAMACGGTA 580

BLAST of IVF0009618 vs. NCBI nr
Match: XP_008451098.1 (PREDICTED: L-ascorbate oxidase-like [Cucumis melo])

HSP 1 Score: 1303 bits (3373), Expect = 0.0
Identity = 626/631 (99.21%), Postives = 627/631 (99.37%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGE Q ILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNF IANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFGEGKFS ADETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGEGKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631

BLAST of IVF0009618 vs. NCBI nr
Match: CAA71275.1 (L-ascorbate oxidase [Cucumis melo])

HSP 1 Score: 1299 bits (3361), Expect = 0.0
Identity = 624/631 (98.89%), Postives = 626/631 (99.21%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60
           MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG
Sbjct: 1   MAKVANQPLFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLG 60

Query: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120
           INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG
Sbjct: 61  INGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPG 120

Query: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180
           ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH
Sbjct: 121 ENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWH 180

Query: 181 TSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240
           TSIQEQEVGLKSNPMRWIGE Q ILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV
Sbjct: 181 TSIQEQEVGLKSNPMRWIGELQVILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVLKV 240

Query: 241 EPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300
           EPNKTYRIRIASTTSLSALNF IANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT
Sbjct: 241 EPNKTYRIRIASTTSLSALNFAIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTT 300

Query: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360
           NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN
Sbjct: 301 NQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTN 360

Query: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420
           RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS
Sbjct: 361 RVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNS 420

Query: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480
           TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP
Sbjct: 421 TFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHP 480

Query: 481 WHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540
           WHLHGHDFWVLGFG+ KFS ADETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWIFH
Sbjct: 481 WHLHGHDFWVLGFGDFKFSAADETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWIFH 540

Query: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600
           CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF
Sbjct: 541 CHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIPYF 600

Query: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631
           MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT
Sbjct: 601 MLWVIGLVIIVCGIGLFGVCLYRIGLRRGYT 631

BLAST of IVF0009618 vs. NCBI nr
Match: XP_011660094.1 (L-ascorbate oxidase-like isoform X1 [Cucumis sativus] >KAE8653486.1 hypothetical protein Csa_007106 [Cucumis sativus])

HSP 1 Score: 1173 bits (3035), Expect = 0.0
Identity = 567/626 (90.58%), Postives = 591/626 (94.41%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GEPQTILINGTGQFNCTSL+KYNTSSEHCEWKDQCAPFVL
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQTILINGTGQFNCTSLEKYNTSSEHCEWKDQCAPFVL 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300
           KVEPNKTYRIRIASTTSL+ALNF I+NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 KVEPNKTYRIRIASTTSLAALNFAISNHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P PP    P   
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNP--- 600

Query: 601 YFMLWVIGLVIIVCGIGLFGVCLYRI 624
           Y+++W IGLVI +C IGL  VCLYRI
Sbjct: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI 622

BLAST of IVF0009618 vs. NCBI nr
Match: XP_031736736.1 (L-ascorbate oxidase-like isoform X2 [Cucumis sativus])

HSP 1 Score: 1024 bits (2647), Expect = 0.0
Identity = 509/626 (81.31%), Postives = 530/626 (84.66%), Query Frame = 0

Query: 1   MAKVANQP-LFPLPFLV-FFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIV 60
           MAKVANQP L P+PFLV FFV SI FGFGITLSKAVSQTRYYKFEV+YMFWSPDC+ENIV
Sbjct: 1   MAKVANQPSLIPMPFLVIFFVFSIFFGFGITLSKAVSQTRYYKFEVEYMFWSPDCIENIV 60

Query: 61  LGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPIN 120
           +GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRG+PWADGTASISQCPIN
Sbjct: 61  MGINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGSPWADGTASISQCPIN 120

Query: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180
           PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW
Sbjct: 121 PGENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDW 180

Query: 181 WHTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWKDQCAPFVL 240
           WH S+ EQEVGLKSNPMRW+GEPQ                                    
Sbjct: 181 WHASVHEQEVGLKSNPMRWVGEPQ------------------------------------ 240

Query: 241 KVEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLV 300
                                     NHTMLVVEADGNYV  F TSDIDIYSGESYSVLV
Sbjct: 241 --------------------------NHTMLVVEADGNYVQQFVTSDIDIYSGESYSVLV 300

Query: 301 TTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSF 360
           TTNQ+TTQNYWASIGVRGR+PKTSPALLLFNYLPNSVAKLPISPPPE PKW+DYDRSK+F
Sbjct: 301 TTNQSTTQNYWASIGVRGREPKTSPALLLFNYLPNSVAKLPISPPPEIPKWNDYDRSKNF 360

Query: 361 TNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGL 420
           T RVLASAMNTTKPPPIFTRRISLLNTQN +NG F KWAINNISLTLPSTPYLGAIKFGL
Sbjct: 361 TYRVLASAMNTTKPPPIFTRRISLLNTQNLMNGNFIKWAINNISLTLPSTPYLGAIKFGL 420

Query: 421 NSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEI 480
           NSTFNQT PPETFPKNYNIKIPPPNPNTNTGN VYRFRSGEIVDVILQNANAL+NNTSEI
Sbjct: 421 NSTFNQTAPPETFPKNYNIKIPPPNPNTNTGNSVYRFRSGEIVDVILQNANALSNNTSEI 480

Query: 481 HPWHLHGHDFWVLGFGEGKFSTADETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540
           HPWHLHGHDFWVLG+GEGKFS  DETKLNL+NPPLKNTVVIFPFGWTAIRFVADNPGVWI
Sbjct: 481 HPWHLHGHDFWVLGYGEGKFSDEDETKLNLKNPPLKNTVVIFPFGWTAIRFVADNPGVWI 540

Query: 541 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTATHNVVPIIPQPKPPRGPGPVIP 600
           FHCHIEPHLDMGMGVIFAEAVE+VREIPPYALACGRTAT  VVPIIP+P PP    P   
Sbjct: 541 FHCHIEPHLDMGMGVIFAEAVEKVREIPPYALACGRTATPQVVPIIPRPNPPGLDNP--- 560

Query: 601 YFMLWVIGLVIIVCGIGLFGVCLYRI 624
           Y+++W IGLVI +C IGL  VCLYRI
Sbjct: 601 YYIIW-IGLVIFLCVIGLCYVCLYRI 560

BLAST of IVF0009618 vs. NCBI nr
Match: XP_038880052.1 (L-ascorbate oxidase-like [Benincasa hispida])

HSP 1 Score: 927 bits (2397), Expect = 0.0
Identity = 444/580 (76.55%), Postives = 494/580 (85.17%), Query Frame = 0

Query: 1   MAKVANQPLFPLPFLVFFVL-SIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVL 60
           MAK ANQPLFPLPFLV F++ S+I GFGITLS+AV+ TRY+K+EV+YMFWSPDCVEN+V+
Sbjct: 1   MAKAANQPLFPLPFLVIFLIFSMISGFGITLSEAVNNTRYFKWEVEYMFWSPDCVENLVM 60

Query: 61  GINGTFPGPPIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINP 120
           GING FPGP IRANV DT++VE++N L TEGVVIHWHGILQR TPWADGTASISQC INP
Sbjct: 61  GINGQFPGPTIRANVGDTIIVELVNKLFTEGVVIHWHGILQRETPWADGTASISQCAINP 120

Query: 121 GENFTYEFKVDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWW 180
           GE F Y+FK DKPGTYFYHGH GMQR+AGLYGSLI+D EEGKKEPF YDEEINLLLSDWW
Sbjct: 121 GETFIYQFKADKPGTYFYHGHLGMQRSAGLYGSLIIDPEEGKKEPFHYDEEINLLLSDWW 180

Query: 181 HTSIQEQEVGLKSNPMRWIGEPQTILINGTGQFNCTSLDKYNTSSEHCEWK--DQCAPFV 240
           H S  +QEVGL S+PMRWIGEP+TILING GQ +C+   KYN + E C+ K  +QCAPF+
Sbjct: 181 HKSAHKQEVGLSSSPMRWIGEPETILINGRGQISCSIAAKYNKNLEQCKLKGGEQCAPFI 240

Query: 241 LKVEPNKTYRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVL 300
           L+V+P KTYRIRIASTTSLSALN  I NH +LVVEADGNYV+PF TSDIDIYSGESYSVL
Sbjct: 241 LQVKPKKTYRIRIASTTSLSALNLAIGNHQLLVVEADGNYVEPFVTSDIDIYSGESYSVL 300

Query: 301 VTTNQATTQNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKS 360
           +TTNQ   QNYW S+GVRGR PKT PAL L NYLPNSVAKLP+SPPPETP W+DY RSK+
Sbjct: 301 ITTNQDPAQNYWVSVGVRGRLPKTPPALTLLNYLPNSVAKLPVSPPPETPNWEDYARSKN 360

Query: 361 FTNRVLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFG 420
           FT R+LA AM +  PP  F RRI LLNTQNR+NG FTKWAINN+SL+LPSTPYLGAIK G
Sbjct: 361 FTYRILA-AMGSPNPPIKFNRRIFLLNTQNRMNGGFTKWAINNVSLSLPSTPYLGAIKIG 420

Query: 421 LNSTFNQTPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSE 480
           LN+TFNQ PPPETF ++Y+I  PPPNPNT T NGVY F  GE VDVILQNAN L +  SE
Sbjct: 421 LNNTFNQNPPPETFSEDYDIFNPPPNPNTITDNGVYMFNLGETVDVILQNANTLNDKHSE 480

Query: 481 IHPWHLHGHDFWVLGFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGV 540
           IHPWHLHGHDFWVLG+GEGKFS A DE KLNL+NPPL+NTVVIFP+GWTAIRFVADNPGV
Sbjct: 481 IHPWHLHGHDFWVLGYGEGKFSAAVDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGV 540

Query: 541 WIFHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRTA 576
           W FHCHIEPHL MGMGVIFAE V +V  IP  ALACG TA
Sbjct: 541 WAFHCHIEPHLHMGMGVIFAEGVHKVGTIPTKALACGGTA 579

BLAST of IVF0009618 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 732.6 bits (1890), Expect = 2.6e-211
Identity = 338/565 (59.82%), Postives = 428/565 (75.75%), Query Frame = 0

Query: 16  VFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVN 75
           V++++ ++     T S AV   R Y +EV+Y +WSPDC E  V+ +NG FPGP I+A   
Sbjct: 19  VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78

Query: 76  DTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGTY 135
           DT+VV + N L+TEG+VIHWHGI Q G+PWADG A ++QC INPGE FTY F V+KPGT+
Sbjct: 79  DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138

Query: 136 FYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPM 195
           FYHGH+GMQR+AGLYGSLIVD+ +GK E  +YD E NLLLSDWWH +I  QE+GL S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198

Query: 196 RWIGEPQTILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 255
           RWIGE Q+ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258

Query: 256 STTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 315
           STT+L++LN  +  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318

Query: 316 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 375
           +GVRGRKP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPS 378

Query: 376 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 435
           PP  + +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++
Sbjct: 379 PPKKYRKRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSY 438

Query: 436 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 495
             +Y+I  PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVL
Sbjct: 439 RMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 498

Query: 496 GFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMG 555
           G+G+GKF    DE   NL+NPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MG
Sbjct: 499 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMG 558

Query: 556 MGVIFAEAVERVREIPPYALACGRT 576
           MGV+FAE + R+ ++P  AL CG T
Sbjct: 559 MGVVFAEGLNRIGKVPDEALGCGLT 578

BLAST of IVF0009618 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 691.4 bits (1783), Expect = 6.7e-199
Identity = 331/569 (58.17%), Postives = 416/569 (73.11%), Query Frame = 0

Query: 15  LVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANV 74
           +V+++L+++     + S AV ++ +   EV+Y +W PDC E IV+ ING FPGP I A  
Sbjct: 4   IVWWLLTVVVVAFHSASAAVVESTW---EVEYKYWWPDCKEGIVMAINGQFPGPTIDAVA 63

Query: 75  NDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGT 134
            DTV++ V+N LSTEGVVIHWHGI Q+GTPWADG A ++QCPINPGE FTY+F VDK GT
Sbjct: 64  GDTVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGT 123

Query: 135 YFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNP 194
           +FYHGH+GMQR++GLYG LIV      KE   YD E NLLLSDWWH SI  QE+ L S P
Sbjct: 124 HFYHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRP 183

Query: 195 MRWIGEPQTILINGTGQFNCTSLDKYNTSSEH--CEWK--DQCAPFVLKVEPNKTYRIRI 254
           MRWIGEPQ++LING GQFNC+    +N   E   C +K  DQCAP  L+VEPN+ YR+RI
Sbjct: 184 MRWIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRI 243

Query: 255 ASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWA 314
           ASTT+L++LN  +  H ++VVEADGNYV PF  +DID+YSGE+YSVL+ TN   ++ YW 
Sbjct: 244 ASTTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWI 303

Query: 315 SIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTT 374
           S+GVRGR+PKT  AL + NY+ ++    P  PPP TP W+D DRSKSF+ ++ A A    
Sbjct: 304 SVGVRGREPKTPQALTVINYV-DATESRPSHPPPVTPIWNDTDRSKSFSKKIFA-AKGYP 363

Query: 375 KPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFN-QTPPPE 434
           KPP     ++ LLNTQN +   +TKW+INN+SL++P TPYLG+I++GL S ++ ++P  +
Sbjct: 364 KPPEKSHDQLILLNTQN-LYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKK 423

Query: 435 TFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFW 494
               NY+I  PPPNPNT  G+G+Y F  G +VDVILQNAN L    SEIHPWH+HGHDFW
Sbjct: 424 LIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFW 483

Query: 495 VLGFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLD 554
           VLG+GEGKF    DE   NL+NPPL+NTVV++PFGWTAIRFV DNPGVW FHCHIEPHL 
Sbjct: 484 VLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLH 543

Query: 555 MGMGVIFAEAVERV--REIPPYALACGRT 576
           MGMGV+F E V+R+   EIP  AL CG T
Sbjct: 544 MGMGVVFVEGVDRIGKMEIPDEALGCGLT 563

BLAST of IVF0009618 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 670.6 bits (1729), Expect = 1.2e-192
Identity = 312/525 (59.43%), Postives = 397/525 (75.62%), Query Frame = 0

Query: 16  VFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGPPIRANVN 75
           V++++ ++     T S AV   R Y +EV+Y +WSPDC E  V+ +NG FPGP I+A   
Sbjct: 19  VWWIVLVVAVLTHTASAAV---REYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAG 78

Query: 76  DTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFKVDKPGTY 135
           DT+VV + N L+TEG+VIHWHGI Q G+PWADG A ++QC INPGE FTY F V+KPGT+
Sbjct: 79  DTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTH 138

Query: 136 FYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEVGLKSNPM 195
           FYHGH+GMQR+AGLYGSLIVD+ +GK E  +YD E NLLLSDWWH +I  QE+GL S PM
Sbjct: 139 FYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSSKPM 198

Query: 196 RWIGEPQTILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIA 255
           RWIGE Q+ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++
Sbjct: 199 RWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLS 258

Query: 256 STTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWAS 315
           STT+L++LN  +  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S
Sbjct: 259 STTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYIS 318

Query: 316 IGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTK 375
           +GVRGRKP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  
Sbjct: 319 VGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPS 378

Query: 376 PPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETF 435
           PP  + +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++
Sbjct: 379 PPKKYRKRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSY 438

Query: 436 PKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVL 495
             +Y+I  PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVL
Sbjct: 439 RMDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVL 498

Query: 496 GFGEGKFSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPG 536
           G+G+GKF    DE   NL+NPPL+NT +++P+GWTAIRFV DNPG
Sbjct: 499 GYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

BLAST of IVF0009618 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 535.8 bits (1379), Expect = 4.7e-152
Identity = 267/577 (46.27%), Postives = 359/577 (62.22%), Query Frame = 0

Query: 9   LFPLPFLVFFVLSIIFGFGITLSKAVSQTRYYKFEVDYMFWSPDCVENIVLGINGTFPGP 68
           L  L F+  F  S++            + R +K+EV Y F SPDC E +V+ ING FPGP
Sbjct: 16  LMVLCFIALFFSSVL---------CQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGP 75

Query: 69  PIRANVNDTVVVEVINNLSTEGVVIHWHGILQRGTPWADGTASISQCPINPGENFTYEFK 128
            I+A   DT+VVE+ N+  TE V +HWHGI Q GTPW DG   ++QCPI PGE F Y+F 
Sbjct: 76  TIKAQQGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFV 135

Query: 129 VDKPGTYFYHGHFGMQRAAGLYGSLIVDLEEGKKEPFQYDEEINLLLSDWWHTSIQEQEV 188
           VD+PGTY YH H+GMQR +GL G + V     + EPF YD + N LL+DW+H S+ E+  
Sbjct: 136 VDRPGTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKAT 195

Query: 189 GLKSNPMRWIGEPQTILINGTGQFNCTS--LDKYNTSSEHCEWKD-QCAPFVLKVEPNKT 248
           GL S P +W+GEPQ+++I G G+FNC++      +  S  C   +  C+ F+L V P KT
Sbjct: 196 GLASIPFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKT 255

Query: 249 YRIRIASTTSLSALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATT 308
           YR+RI S T+LSAL+F I  H + VVEADG+YV+PF   ++ +YSGE+YSVL+  +Q   
Sbjct: 256 YRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPR 315

Query: 309 QNYWASIGVRGRKPKTSPALLLFNYLPNSVAKLPISPPPET----PKWDDYDRSKSFTNR 368
           +NYW +  +  R   T PA  + NY PN   + P  P  E+    P+W+D     + +  
Sbjct: 316 RNYWITSSIVSRPATTPPATAVLNYYPNHPRRRP--PTSESSNIVPEWNDTRSRLAQSLA 375

Query: 369 VLASAMNTTKPPPIFTRRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNST 428
           + A        P    + I LLNTQN +NG + +W++NN+S   P TPYL A+K  L + 
Sbjct: 376 IKARRGFIHALPENSDKVIVLLNTQNEVNG-YRRWSVNNVSYHHPKTPYLIALKQNLTNA 435

Query: 429 FNQ--TPPPETFPKNYNIKIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIH 488
           F+   T P     +NY+I   P N N  T +G+YR R    VDVILQNAN +  N SE H
Sbjct: 436 FDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETH 495

Query: 489 PWHLHGHDFWVLGFGEGKFSTADETK-LNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWI 548
           PWHLHGHDFWVLG+GEGKF+ +++ K  N  +P  KNTV + PFGWTA+RF ADNPGVW 
Sbjct: 496 PWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWS 555

Query: 549 FHCHIEPHLDMGMGVIFAEAVERVREIPPYALACGRT 576
           FHCHIE H  MGMG++F   +++V  +P   + CG+T
Sbjct: 556 FHCHIESHFFMGMGIVFESGIDKVSSLPSSIMGCGQT 580

BLAST of IVF0009618 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 485.0 bits (1247), Expect = 9.5e-137
Identity = 225/379 (59.37%), Postives = 288/379 (75.99%), Query Frame = 0

Query: 202 QTILINGTGQFNCTSLDKY--NTSSEHCEWK--DQCAPFVLKVEPNKTYRIRIASTTSLS 261
           ++ILING GQFNC+   ++  NTS   C +K  DQCAP +L VEPNKTYRIR++STT+L+
Sbjct: 11  KSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALA 70

Query: 262 ALNFGIANHTMLVVEADGNYVDPFETSDIDIYSGESYSVLVTTNQATTQNYWASIGVRGR 321
           +LN  +  H ++VVEADGNY+ PF T DIDIYSGESYSVL+TT+Q  +QNY+ S+GVRGR
Sbjct: 71  SLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGR 130

Query: 322 KPKTSPALLLFNYLPNSVAKLPISPPPETPKWDDYDRSKSFTNRVLASAMNTTKPPPIFT 381
           KP T+ AL + NY+    +KLP SPPP TP+WDD++RSK+F+ ++  SAM +  PP  + 
Sbjct: 131 KPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIF-SAMGSPSPPKKYR 190

Query: 382 RRISLLNTQNRINGKFTKWAINNISLTLPSTPYLGAIKFGLNSTFNQTPPPETFPKNYNI 441
           +R+ LLNTQN I+G +TKWAINN+SL  P+TPYLG++K+ L   FN+  PP ++  +Y+I
Sbjct: 191 KRLILLNTQNLIDG-YTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRMDYDI 250

Query: 442 KIPPPNPNTNTGNGVYRFRSGEIVDVILQNANALTNNTSEIHPWHLHGHDFWVLGFGEGK 501
             PPP PNT TGNG+Y F     VDVI+QNAN L    SEIHPWHLHGHDFWVLG+G+GK
Sbjct: 251 MNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGK 310

Query: 502 FSTA-DETKLNLQNPPLKNTVVIFPFGWTAIRFVADNPGVWIFHCHIEPHLDMGMGVIFA 561
           F    DE   NL+NPPL+NT +++P+GWTAIRFV DNPGVW FHCHIEPHL MGMGV+FA
Sbjct: 311 FKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFA 370

Query: 562 EAVERVREIPPYALACGRT 576
           E + R+ ++P  AL CG T
Sbjct: 371 EGLNRIGKVPDEALGCGLT 387

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P141334.6e-26173.01L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1[more]
P247927.9e-24570.62L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2[more]
P370642.0e-24069.71L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1[more]
Q405885.9e-21662.39L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1[more]
M4DUF24.5e-20860.14L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3BQ560.0e+0099.21L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103492477 PE=3 SV=1[more]
Q9ZR590.0e+0098.89L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=ao3 PE=3 SV=1[more]
A0A0A0M2J60.0e+0089.17L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_1G602530 PE=3 SV=1[more]
Q9M5B51.1e-26876.30L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=2C07 PE=2 SV=1[more]
A0A5D3CF112.6e-26775.95L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G0... [more]
Match NameE-valueIdentityDescription
XP_008451098.10.099.21PREDICTED: L-ascorbate oxidase-like [Cucumis melo][more]
CAA71275.10.098.89L-ascorbate oxidase [Cucumis melo][more]
XP_011660094.10.090.58L-ascorbate oxidase-like isoform X1 [Cucumis sativus] >KAE8653486.1 hypothetical... [more]
XP_031736736.10.081.31L-ascorbate oxidase-like isoform X2 [Cucumis sativus][more]
XP_038880052.10.076.55L-ascorbate oxidase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G21105.12.6e-21159.82Plant L-ascorbate oxidase [more]
AT5G21100.16.7e-19958.17Plant L-ascorbate oxidase [more]
AT5G21105.31.2e-19259.43Plant L-ascorbate oxidase [more]
AT4G39830.14.7e-15246.27Cupredoxin superfamily protein [more]
AT5G21105.29.5e-13759.37Plant L-ascorbate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 44..157
e-value: 5.3E-41
score: 139.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 165..368
e-value: 4.3E-62
score: 211.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 370..581
e-value: 4.2E-74
score: 250.8
IPR008972CupredoxinGENE3D2.60.40.420coord: 27..156
e-value: 6.2E-48
score: 164.8
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 32..181
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 164..365
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 375..578
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 171..331
e-value: 1.1E-48
score: 165.4
IPR017760L-ascorbate oxidase, plantsTIGRFAMTIGR03388TIGR03388coord: 38..575
e-value: 1.1E-279
score: 926.6
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 443..558
e-value: 2.4E-36
score: 124.5
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 31..576
NoneNo IPR availablePANTHERPTHR11709:SF394PLANT L-ASCORBATE OXIDASEcoord: 31..576
IPR002355Multicopper oxidase, copper-binding sitePROSITEPS00080MULTICOPPER_OXIDASE2coord: 540..551
IPR033138Multicopper oxidases, conserved sitePROSITEPS00079MULTICOPPER_OXIDASE1coord: 535..555
IPR034267Ascorbate oxidase, third cupredoxin domainCDDcd13893CuRO_3_AAOcoord: 376..566
e-value: 1.53673E-76
score: 239.244
IPR034259Ascorbate oxidase, first cupredoxin domainCDDcd13845CuRO_1_AAOcoord: 38..157
e-value: 1.35338E-77
score: 240.426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009618.1IVF0009618.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0008447 L-ascorbate oxidase activity
molecular_function GO:0016491 oxidoreductase activity