IVF0009561 (gene) Melon (IVF77) v1

Overview
NameIVF0009561
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionDelta(8)-fatty-acid desaturase
Locationchr11: 28213929 .. 28216660 (-)
RNA-Seq ExpressionIVF0009561
SyntenyIVF0009561
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTGAGTGAGCCGCGGGTGAGGTTAAAAGGAGGGAAGCCGATTTGAGATGCGGGGGAAACGGCCCCTAAAGCCTAAAGCCCAGGTTGGTTTTTGTACCGTGTACTTCGCTGTTAACAATCACGACCTCTCATTCTTCTCTGTTCCTTCATTCGTATCCGTTTCATCTTTTTCTATATTAAACCCTTCTAAATGCAACATGCAATTCAGAAAGAGGGGTTCACACTATTCTAGATTCTGAATCAATCCAACCCAATTTCCCCCCCTCTCTTCTCCGAATAGTGGGTTGCATTTTCCCATCCAAAGCAACCCCACGATAAAAAATAAACGAAGAAATTTCTTCTTATACCTTCAAAGAAAACCTTTCTCGCTTTGATTCTCATCTGGGTTTCTTCTGTTTTCTTTGTTTGATCTCCTTTTACCAAACTTTGAAGGTCTGGTTTGGATATGGAGGTGGAGAAGAAGTACATTACCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTATGGATCTCTATTCAGGGTAAGGTCTATAATGTCACAGAATGGGCTAAAGTACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATTGCTTATCATCCTGGCACCGCTTGGGCGTATTTGGATCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGAAAGCTTGCTTCTGAATTTTCCAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTATTATGTATTCTTTGATTTCCGTTGCCGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTAATGTCGAGTCGTGGATTCAACAAATTGGCTCAAATTTTGTCTGGAAATTGTTTGACGGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCACATCTCTTGCAATAGTCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCCGTTTCTGCTACTTTCTTCCGGACTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTTGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAGACATTGTTGCTCTTATTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCCTATCTCCCTAACTGGCCTGAGAGAGTCATGTTTGTTCTTGCAAGTTTTGCTGTATGTTCTCTACAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGATTGGTTCGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCATCTTGGATGGATTGGTTTTTTGGTGGATTGCAATTTCAGTTAGAACATCATTTATTCCCAAGGATGCCCAGATGCCAGTTGAGGAAAATCTCTCCAATCGTGGTCGAACTTTGCAAGAAACACAATCTTCCATACAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAAGCTAGAGACTTGACCAATCCTGTTCCTAAGAACTTGCTCTGGGAAGCCGTGAACACTCATGGATGAAGATGAAGTTAAAGATGAGGTCAGATCCTTCATATTTCTTATCTAGACTTCTCAAGAACGGCAGCAGGTTCTTTTTTTAATTTTTTTCTCCTCCCTTTTGTTTGTTTATTTTGATGGGCTTACTGTTATTTTTCATATTATGGGCCTCTTAGGATATGTTTGAACTCTGCTTAAAACATTACACTCAGCTTCCTCTTGTTGGTTCTTTTATTTGATTACCATAAATTTGCCTTTCTATTCTCTCTGCTGAACTTCTGAATTGAATGAAAAACTGCTATATAAAATCCAATCCATGATCTGGGTTTCTTTGAATTTGAACTTCCCTGTTTTTAGAATATACAGCAATGGCGTTTTTTAACTTCTTGTTTATTGAGAGAGAAGAGAATATGCCATTTTTATTTAAAGATCCGATTTGTGTGTTTACTGTGAAAAGGAACTGGGGACTGTCCCCATTTGGACAGTTGTGTCTTGTGATTTCGAATCTTTTCTTTTATGTCCCCATTGTATTGCTTTGTTGAGATTTTGAGTTGAAAATGGTCTGTTTCTGTTTTACCATTGGGAGTTTTGGCCCTACCTCACACATGGCCACCTCACTGCCTTACTCTGGCCTGTCTAAGGTGTGGCTCACCACTCCCCTTCTCTTCTTTTTAGGTCACTTCCGGGCTCGTGTAGAGCATGGTTTGAATGTCGATGGTTCGATTTGGTTCGAGCGAACTCATTAGAAAATTGCATAGTCCAATCAAATGAGACTTCCAAGTCTACATACTACAGATCTAAAGCGCTTTATTGCATTTATGAGTAAATTAATTACAATTCCAATTCCAATTTAGAGAAGAAAAATAAAATCTTCTTGATCTCTTGTATAAATACAGGTAAATTTGTTTCCATGTCTTTCAATGACTCCTATATTCTTTAAATGTTAATTGAATCTATGTTGTTCAA

mRNA sequence

ATTTGAGTGAGCCGCGGGTGAGGTTAAAAGGAGGGAAGCCGATTTGAGATGCGGGGGAAACGGCCCCTAAAGCCTAAAGCCCAGGTTGGTTTTTGTACCGTGTACTTCGCTGTTAACAATCACGACCTCTCATTCTTCTCTGTTCCTTCATTCGTATCCGTTTCATCTTTTTCTATATTAAACCCTTCTAAATGCAACATGCAATTCAGAAAGAGGGGTTCACACTATTCTAGATTCTGAATCAATCCAACCCAATTTCCCCCCCTCTCTTCTCCGAATAGTGGGTTGCATTTTCCCATCCAAAGCAACCCCACGATAAAAAATAAACGAAGAAATTTCTTCTTATACCTTCAAAGAAAACCTTTCTCGCTTTGATTCTCATCTGGGTTTCTTCTGTTTTCTTTGTTTGATCTCCTTTTACCAAACTTTGAAGGTCTGGTTTGGATATGGAGGTGGAGAAGAAGTACATTACCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTATGGATCTCTATTCAGGGTAAGGTCTATAATGTCACAGAATGGGCTAAAGTACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATTGCTTATCATCCTGGCACCGCTTGGGCGTATTTGGATCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGAAAGCTTGCTTCTGAATTTTCCAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTATTATGTATTCTTTGATTTCCGTTGCCGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTAATGTCGAGTCGTGGATTCAACAAATTGGCTCAAATTTTGTCTGGAAATTGTTTGACGGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCACATCTCTTGCAATAGTCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCCGTTTCTGCTACTTTCTTCCGGACTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTTGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAGACATTGTTGCTCTTATTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCCTATCTCCCTAACTGGCCTGAGAGAGTCATGTTTGTTCTTGCAAGTTTTGCTGTATGTTCTCTACAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGATTGGTTCGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCATCTTGGATGGATTGGTTTTTTGGTGGATTGCAATTTCAGTTAGAACATCATTTATTCCCAAGGATGCCCAGATGCCAGTTGAGGAAAATCTCTCCAATCGTGGTCGAACTTTGCAAGAAACACAATCTTCCATACAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAAGCTAGAGACTTGACCAATCCTGTTCCTAAGAACTTGCTCTGGGAAGCCGTGAACACTCATGGATGAAGATGAAGTTAAAGATGAGGTCAGATCCTTCATATTTCTTATCTAGACTTCTCAAGAACGGCAGCAGGTTCTTTTTTTAATTTTTTTCTCCTCCCTTTTGTTTGTTTATTTTGATGGGCTTACTGTTATTTTTCATATTATGGGCCTCTTAGGATATGTTTGAACTCTGCTTAAAACATTACACTCAGCTTCCTCTTGTTGGTTCTTTTATTTGATTACCATAAATTTGCCTTTCTATTCTCTCTGCTGAACTTCTGAATTGAATGAAAAACTGCTATATAAAATCCAATCCATGATCTGGGTTTCTTTGAATTTGAACTTCCCTGTTTTTAGAATATACAGCAATGGCGTTTTTTAACTTCTTGTTTATTGAGAGAGAAGAGAATATGCCATTTTTATTTAAAGATCCGATTTGTGTGTTTACTGTGAAAAGGAACTGGGGACTGTCCCCATTTGGACAGTTGTGTCTTGTGATTTCGAATCTTTTCTTTTATGTCCCCATTGTATTGCTTTGTTGAGATTTTGAGTTGAAAATGGTCTGTTTCTGTTTTACCATTGGGAGTTTTGGCCCTACCTCACACATGGCCACCTCACTGCCTTACTCTGGCCTGTCTAAGGTGTGGCTCACCACTCCCCTTCTCTTCTTTTTAGGTCACTTCCGGGCTCGTGTAGAGCATGGTTTGAATGTCGATGGTTCGATTTGGTTCGAGCGAACTCATTAGAAAATTGCATAGTCCAATCAAATGAGACTTCCAAGTCTACATACTACAGATCTAAAGCGCTTTATTGCATTTATGAGTAAATTAATTACAATTCCAATTCCAATTTAGAGAAGAAAAATAAAATCTTCTTGATCTCTTGTATAAATACAGGTAAATTTGTTTCCATGTCTTTCAATGACTCCTATATTCTTTAAATGTTAATTGAATCTATGTTGTTCAA

Coding sequence (CDS)

ATGGAGGTGGAGAAGAAGTACATTACCTCTGAGGAGCTTCTCAAGCACAACAAACCAGGAGATCTATGGATCTCTATTCAGGGTAAGGTCTATAATGTCACAGAATGGGCTAAAGTACATCCTGGTGGTGATACGCCTTTTCTCAATTTGGCGGGTCATGATGTTACCGATGCATTCATTGCTTATCATCCTGGCACCGCTTGGGCGTATTTGGATCGGTTGTTCACTGGATTTCATCTCAAGGATTTTCAGGTCTCGGAGATTTCTAAGGATTACAGAAAGCTTGCTTCTGAATTTTCCAAACAAGGTTTGTTTGAGAAGAAGGGGCATGTTATTATGTATTCTTTGATTTCCGTTGCCGTGATGATGTTTCTTGTGGTTTATGGGGTTTTGAAATCTGAGAGTGTTTTGGTTCATTTGGGTTGTGCTATGATTTTGGGTATGCTTTGGATCCAAAGTGCTTATGTGGGTCATGATTCTGGACATTCTAATGTAATGTCGAGTCGTGGATTCAACAAATTGGCTCAAATTTTGTCTGGAAATTGTTTGACGGGGATTAGTATAGCCTGGTGGAAATGGACTCATAATGCTCATCACATCTCTTGCAATAGTCTTGACCATGATCCAGATCTTCAACACATCCCTGTTTTTGCCGTTTCTGCTACTTTCTTCCGGACTTTGACATCTCATTTCTATGGTAGGAAGTTGAAGTTTGATTCCTTGGCTAGATTCTTTGTTAGTTACCAGCATTTCACATTTTACCCTGTGATGTGTGTGGCTAGAGTGAATCTCTTTGTGCAGACATTGTTGCTCTTATTTTCCACTAGAAAAGTCCCTGATAGAGCATTGAACATAATGGGGATTCTTGTGTTTTGGACATGGTTTCCTCTTCTAGTTTCCTATCTCCCTAACTGGCCTGAGAGAGTCATGTTTGTTCTTGCAAGTTTTGCTGTATGTTCTCTACAGCACATCCAATTCTGCCTCAACCATTTCTCTGCTAATGTTTATGTTGGGAAGCCCACTGGGAATGATTGGTTCGAGAAGCAAACAAGTGGGACTTTGGATATCTCTTGTTCATCTTGGATGGATTGGTTTTTTGGTGGATTGCAATTTCAGTTAGAACATCATTTATTCCCAAGGATGCCCAGATGCCAGTTGAGGAAAATCTCTCCAATCGTGGTCGAACTTTGCAAGAAACACAATCTTCCATACAGAAGCTTGTCATTTTGGGATGCTAATGTCCGCACCATTAGAACTCTTAGAACAGCTGCTTTGCAAGCTAGAGACTTGACCAATCCTGTTCCTAAGAACTTGCTCTGGGAAGCCGTGAACACTCATGGATGA

Protein sequence

MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAALQARDLTNPVPKNLLWEAVNTHG
Homology
BLAST of IVF0009561 vs. ExPASy Swiss-Prot
Match: Q3EBF7 (Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 1.9e-208
Identity = 329/446 (73.77%), Postives = 386/446 (86.55%), Query Frame = 0

Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIA 61
           + +K+Y+TSE+L KHNKPGDLWISIQGKVY+V++W K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAV 121
           YHPGTAW +L++L  G+H++D  VS++S+DYR+LA+EFSK+GLF+KKGHV +Y+L  V V
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M+  V+YGVL   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF ++TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSW 361
           LPNW ER +FV  SFAV ++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC S+
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRSLS+W+ANV TIRTL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

BLAST of IVF0009561 vs. ExPASy Swiss-Prot
Match: Q9ZRP7 (Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV=1)

HSP 1 Score: 715.7 bits (1846), Expect = 3.3e-205
Identity = 326/446 (73.09%), Postives = 380/446 (85.20%), Query Frame = 0

Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIA 61
           E EKKYIT+E+L KHNK GDLWI+IQGKVYNV++W K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAV 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYR++A+EF K GLFE KGHV +Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M   V+YGVL   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF +LTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVFWTWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSW 361
           LPNWPER  FV  SF V +LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DISC S+
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRS+S+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

BLAST of IVF0009561 vs. ExPASy Swiss-Prot
Match: Q43469 (Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 5.2e-203
Identity = 322/443 (72.69%), Postives = 379/443 (85.55%), Query Frame = 0

Query: 5   KKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYITS+EL KHN P DLWISI GKVYNVTEWAK HPGGD P +NLAG DVTDAFIA+HP
Sbjct: 16  KKYITSKELKKHNNPNDLWISILGKVYNVTEWAKEHPGGDAPLINLAGQDVTDAFIAFHP 75

Query: 65  GTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVMMF 124
           GTAW +LD+LFTG+HLKD+QVS+IS+DYRKLASEF+K G+FEKKGH ++YSL  V++++ 
Sbjct: 76  GTAWKHLDKLFTGYHLKDYQVSDISRDYRKLASEFAKAGMFEKKGHGVIYSLCFVSLLLS 135

Query: 125 LVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 184
             VYGVL S S  +H+    ILG+ W+Q AY+GHD+GH  +M++RG+NK A I  GNC+T
Sbjct: 136 ACVYGVLYSGSFWIHMLSGAILGLAWMQIAYLGHDAGHYQMMATRGWNKFAGIFIGNCIT 195

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLARF 244
           GISIAWWKWTHNAHHI+CNSLD+DPDLQH+P+ AVS+  F ++TS FYGR+L FD LARF
Sbjct: 196 GISIAWWKWTHNAHHIACNSLDYDPDLQHLPMLAVSSKLFNSITSVFYGRQLTFDPLARF 255

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 304
           FVSYQH+ +YP+MCVARVNL++QT+LLL S RK+PDR LNI+G L+FWTWFPLLVS LPN
Sbjct: 256 FVSYQHYLYYPIMCVARVNLYLQTILLLISKRKIPDRGLNILGTLIFWTWFPLLVSRLPN 315

Query: 305 WPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWMDW 364
           WPERV FVL SF V  +QHIQF LNHFS +VYVG P G++WFEKQT GT+DI+CSSWMDW
Sbjct: 316 WPERVAFVLVSFCVTGIQHIQFTLNHFSGDVYVGPPKGDNWFEKQTRGTIDIACSSWMDW 375

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 424
           FFGGLQFQLEHHLFPR+PRC LR ISPI  ELCKK+NLPY SLSF+DANV T++TLRTAA
Sbjct: 376 FFGGLQFQLEHHLFPRLPRCHLRSISPICRELCKKYNLPYVSLSFYDANVTTLKTLRTAA 435

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARDLTNP P+NL WEA NTHG
Sbjct: 436 LQARDLTNPAPQNLAWEAFNTHG 458

BLAST of IVF0009561 vs. ExPASy Swiss-Prot
Match: Q9FR82 (Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 6.8e-203
Identity = 333/447 (74.50%), Postives = 384/447 (85.91%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           ME  KKYI+  EL KHN+ GD+WISIQGKVYNVT+W K HPGGD P +NLAG D TDAFI
Sbjct: 1   MEGTKKYISVGELEKHNQLGDVWISIQGKVYNVTDWIKKHPGGDVPIMNLAGQDATDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAW  L+ LFTG+HL+D+ VSEISKDYRKLASEFSK GLFEKKGH ++Y L  +A
Sbjct: 61  AYHPGTAWKNLENLFTGYHLEDYLVSEISKDYRKLASEFSKAGLFEKKGHTVIYCLSFIA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +++   VYGVL S S+ VH+    +LGM +IQ+AY+GHDSGH  +MSS+G+NK AQ+L+G
Sbjct: 121 LLLCGCVYGVLCSNSLWVHMLSGAMLGMCFIQAAYLGHDSGHYTMMSSKGYNKFAQVLNG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHI+CNSLD+DPDLQH+PVFAV ++FF++LTS FYGR+L FD 
Sbjct: 181 NCLTGISIAWWKWTHNAHHIACNSLDYDPDLQHLPVFAVPSSFFKSLTSRFYGRELTFDG 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           L+RF VSYQHFT Y VM   R+NL+VQT LLLFSTRKVPDRALNI+GILV+WTWFP LVS
Sbjct: 241 LSRFLVSYQHFTIYLVMIFGRINLYVQTFLLLFSTRKVPDRALNIIGILVYWTWFPYLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
            LPNW ERV+FVL  F+V +LQHIQF LNHF+A+VYVG PTG +WFEKQ +GT+DISCSS
Sbjct: 301 CLPNWNERVLFVLTCFSVTALQHIQFTLNHFAADVYVGPPTGTNWFEKQAAGTIDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLR ISPIV + CKKHNLPYRSLSF+DANV TI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRNISPIVQDYCKKHNLPYRSLSFFDANVATIKTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLT  VP+NLLWEA NTHG
Sbjct: 421 RTAALQARDLT-VVPQNLLWEAFNTHG 446

BLAST of IVF0009561 vs. ExPASy Swiss-Prot
Match: O04353 (Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.4e-179
Identity = 283/443 (63.88%), Postives = 353/443 (79.68%), Query Frame = 0

Query: 5   KKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIAYHP 64
           KKYITS+EL  H+KPGDLWISIQGK Y+V++W K HPGG  P  +LAG +VTDAF+A+HP
Sbjct: 6   KKYITSDELKNHDKPGDLWISIQGKAYDVSDWVKDHPGGSFPLKSLAGQEVTDAFVAFHP 65

Query: 65  GTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAVMMF 124
            + W  LD+ FTG++LKD+ VSE+SKDYRKL  EFSK GL++KKGH++  +L  +A++  
Sbjct: 66  ASTWKNLDKFFTGYYLKDYSVSEVSKDYRKLVFEFSKMGLYDKKGHIMFATLCFIAMLFA 125

Query: 125 LVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGNCLT 184
           + VYGVL  E VLVHL    ++G LWIQS ++GHD+GH  V+S    NK   I + NCL+
Sbjct: 126 MSVYGVLFCEGVLVHLFSGCLMGFLWIQSGWIGHDAGHYMVVSDSRLNKFMGIFAANCLS 185

Query: 185 GISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSLARF 244
           GISI WWKW HNAHHI+CNSL++DPDLQ+IP   VS+ FF +LTSHFY ++L FDSL+RF
Sbjct: 186 GISIGWWKWNHNAHHIACNSLEYDPDLQYIPFLVVSSKFFGSLTSHFYEKRLTFDSLSRF 245

Query: 245 FVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSYLPN 304
           FVSYQH+TFYP+MC AR+N++VQ+L++L + R V  RA  ++G LVF  W+PLLVS LPN
Sbjct: 246 FVSYQHWTFYPIMCAARLNMYVQSLIMLLTKRNVSYRAHELLGCLVFSIWYPLLVSCLPN 305

Query: 305 WPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSWMDW 364
           W ER+MFV+AS +V  +Q +QF LNHFS++VYVGKP GN+WFEKQT GTLDISC  WMDW
Sbjct: 306 WGERIMFVIASLSVTGMQQVQFSLNHFSSSVYVGKPKGNNWFEKQTDGTLDISCPPWMDW 365

Query: 365 FFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLRTAA 424
           F GGLQFQ+EHHLFP+MPRC LRKISP V+ELCKKHNLPY   SF  AN  T+RTLR  A
Sbjct: 366 FHGGLQFQIEHHLFPKMPRCNLRKISPYVIELCKKHNLPYNYASFSKANEMTLRTLRNTA 425

Query: 425 LQARDLTNPVPKNLLWEAVNTHG 448
           LQARD+T P+PKNL+WEA++THG
Sbjct: 426 LQARDITKPLPKNLVWEALHTHG 448

BLAST of IVF0009561 vs. ExPASy TrEMBL
Match: A0A5D3CS55 (Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold268G00250 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 4.8e-268
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. ExPASy TrEMBL
Match: A0A1S3C7C0 (delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 4.8e-268
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. ExPASy TrEMBL
Match: A0A0A0KA29 (Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.2e-264
Identity = 439/447 (98.21%), Postives = 443/447 (99.11%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAK HPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFRTLTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. ExPASy TrEMBL
Match: A0A6J1KW79 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111499262 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 7.2e-256
Identity = 423/447 (94.63%), Postives = 435/447 (97.32%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNVT+WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF++LTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

BLAST of IVF0009561 vs. ExPASy TrEMBL
Match: A0A6J1H485 (delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460269 PE=4 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 1.6e-255
Identity = 422/447 (94.41%), Postives = 435/447 (97.32%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNVT+WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYR+LASEFS+QGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF++LTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 448
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

BLAST of IVF0009561 vs. NCBI nr
Match: XP_008458161.1 (PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-fatty-acid desaturase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 924 bits (2389), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. NCBI nr
Match: XP_031743128.1 (delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolipid desaturase [Cucumis sativus] >KGN45699.1 hypothetical protein Csa_005537 [Cucumis sativus])

HSP 1 Score: 912 bits (2356), Expect = 0.0
Identity = 439/447 (98.21%), Postives = 443/447 (99.11%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAK HPGGDTPF+NLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFINLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FFRTLTSHFYGRKL+FDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFRTLTSHFYGRKLEFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQ SGTLDISCS+
Sbjct: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQASGTLDISCST 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. NCBI nr
Match: XP_038874611.1 (delta(8)-fatty-acid desaturase 2 [Benincasa hispida])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 433/447 (96.87%), Postives = 440/447 (98.43%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGF+LKDFQVSE+SKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFYLKDFQVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           VMMFLVVYGVLKSESVLVHL CA+ILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG
Sbjct: 121 VMMFLVVYGVLKSESVLVHLVCALILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSA FFR+LTSHFYGRKLKFD 
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSAKFFRSLTSHFYGRKLKFDG 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGI VFWTWFPLLVS
Sbjct: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGIFVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNW ERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCS+
Sbjct: 301 YLPNWSERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCST 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI+TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIKTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447
           RTAALQARDLTNPVPKNLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447

BLAST of IVF0009561 vs. NCBI nr
Match: XP_023006577.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima])

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 423/447 (94.63%), Postives = 435/447 (97.32%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNVT+WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYR+LASEFSKQGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSKQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF++LTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTKGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

BLAST of IVF0009561 vs. NCBI nr
Match: XP_022959210.1 (delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >KAG7013765.1 Delta(8)-fatty-acid desaturase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 883 bits (2281), Expect = 0.0
Identity = 422/447 (94.41%), Postives = 435/447 (97.32%), Query Frame = 0

Query: 1   MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFI 60
           ME EKKYITSEELLKHNKPGDLWISIQGKVYNVT+WAK HPGGDTPFLNLAGHDVTDAFI
Sbjct: 1   MEAEKKYITSEELLKHNKPGDLWISIQGKVYNVTDWAKEHPGGDTPFLNLAGHDVTDAFI 60

Query: 61  AYHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVA 120
           AYHPGTAWAYLDRLFTGFHL+DF VSE+SKDYR+LASEFS+QGLFEKKGHV MYSLISVA
Sbjct: 61  AYHPGTAWAYLDRLFTGFHLEDFHVSEVSKDYRRLASEFSRQGLFEKKGHVTMYSLISVA 120

Query: 121 VMMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSG 180
           +M+FLVVYGV+KSESVLVHLG AMILGMLWIQSAYVGHDSGHS VMSSRGFNKLAQILSG
Sbjct: 121 IMLFLVVYGVVKSESVLVHLGSAMILGMLWIQSAYVGHDSGHSTVMSSRGFNKLAQILSG 180

Query: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDS 240
           NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVS +FF++LTSHFYGR LKFDS
Sbjct: 181 NCLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSTSFFKSLTSHFYGRTLKFDS 240

Query: 241 LARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300
           LARFF+SYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS
Sbjct: 241 LARFFISYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVS 300

Query: 301 YLPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSS 360
           YLPNWPERVMFVLASFAV SLQHIQFCLNHFSANVYVGKP GNDWFEKQT GTLDISCSS
Sbjct: 301 YLPNWPERVMFVLASFAVTSLQHIQFCLNHFSANVYVGKPIGNDWFEKQTQGTLDISCSS 360

Query: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTL 420
           WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTI TL
Sbjct: 361 WMDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTINTL 420

Query: 421 RTAALQARDLTNPVPKNLLWEAVNTHG 447
           RTAALQARDLTNPVP+NLLWEAVNTHG
Sbjct: 421 RTAALQARDLTNPVPQNLLWEAVNTHG 447

BLAST of IVF0009561 vs. TAIR 10
Match: AT2G46210.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 726.5 bits (1874), Expect = 1.3e-209
Identity = 329/446 (73.77%), Postives = 386/446 (86.55%), Query Frame = 0

Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIA 61
           + +K+Y+TSE+L KHNKPGDLWISIQGKVY+V++W K HPGG+   LNLAG DVTDAFIA
Sbjct: 4   QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAV 121
           YHPGTAW +L++L  G+H++D  VS++S+DYR+LA+EFSK+GLF+KKGHV +Y+L  V V
Sbjct: 64  YHPGTAWHHLEKLHNGYHVRDHHVSDVSRDYRRLAAEFSKRGLFDKKGHVTLYTLTCVGV 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M+  V+YGVL   S+  HL  A++LG+LWIQSAYVGHDSGH  V S++  NKL Q+LSGN
Sbjct: 124 MLAAVLYGVLACTSIWAHLISAVLLGLLWIQSAYVGHDSGHYTVTSTKPCNKLIQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHHI+CNSLDHDPDLQHIP+FAVS  FF ++TS FYGRKL FD L
Sbjct: 184 CLTGISIAWWKWTHNAHHIACNSLDHDPDLQHIPIFAVSTKFFNSMTSRFYGRKLTFDPL 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 301
           ARF +SYQH+TFYPVMCV R+NLF+QT LLLFS R VPDRALNI GILVFWTWFPLLVS+
Sbjct: 244 ARFLISYQHWTFYPVMCVGRINLFIQTFLLLFSKRHVPDRALNIAGILVFWTWFPLLVSF 303

Query: 302 LPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSW 361
           LPNW ER +FV  SFAV ++QH+QFCLNHF+A+VY G P GNDWFEKQT+GTLDISC S+
Sbjct: 304 LPNWQERFIFVFVSFAVTAIQHVQFCLNHFAADVYTGPPNGNDWFEKQTAGTLDISCRSF 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LR +SP+V ELCKKHNLPYRSLS+W+ANV TIRTL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRTVSPVVKELCKKHNLPYRSLSWWEANVWTIRTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
            AA+QARD TNPV KNLLWEAVNTHG
Sbjct: 424 NAAIQARDATNPVLKNLLWEAVNTHG 449

BLAST of IVF0009561 vs. TAIR 10
Match: AT3G61580.1 (Fatty acid/sphingolipid desaturase )

HSP 1 Score: 715.7 bits (1846), Expect = 2.3e-206
Identity = 326/446 (73.09%), Postives = 380/446 (85.20%), Query Frame = 0

Query: 2   EVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAFIA 61
           E EKKYIT+E+L KHNK GDLWI+IQGKVYNV++W K HPGGDT  LNL G DVTDAFIA
Sbjct: 4   ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAFIA 63

Query: 62  YHPGTAWAYLDRLFTGFHLKDFQVSEISKDYRKLASEFSKQGLFEKKGHVIMYSLISVAV 121
           +HPGTAW +LD LFTG+H++DFQVSE+S+DYR++A+EF K GLFE KGHV +Y+L  VA 
Sbjct: 64  FHPGTAWHHLDHLFTGYHIRDFQVSEVSRDYRRMAAEFRKLGLFENKGHVTLYTLAFVAA 123

Query: 122 MMFLVVYGVLKSESVLVHLGCAMILGMLWIQSAYVGHDSGHSNVMSSRGFNKLAQILSGN 181
           M   V+YGVL   SV  H   A +LG+LWIQSAY+GHDSGH  +MS++ +N+ AQ+LSGN
Sbjct: 124 MFLGVLYGVLACTSVFAHQIAAALLGLLWIQSAYIGHDSGHYVIMSNKSYNRFAQLLSGN 183

Query: 182 CLTGISIAWWKWTHNAHHISCNSLDHDPDLQHIPVFAVSATFFRTLTSHFYGRKLKFDSL 241
           CLTGISIAWWKWTHNAHH++CNSLD+DPDLQHIPVFAVS  FF +LTS FY RKL FD +
Sbjct: 184 CLTGISIAWWKWTHNAHHLACNSLDYDPDLQHIPVFAVSTKFFSSLTSRFYDRKLTFDPV 243

Query: 242 ARFFVSYQHFTFYPVMCVARVNLFVQTLLLLFSTRKVPDRALNIMGILVFWTWFPLLVSY 301
           ARF VSYQHFT+YPVMC  R+NLF+QT LLLFS R+VPDRALN  GILVFWTWFPLLVS 
Sbjct: 244 ARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILVFWTWFPLLVSC 303

Query: 302 LPNWPERVMFVLASFAVCSLQHIQFCLNHFSANVYVGKPTGNDWFEKQTSGTLDISCSSW 361
           LPNWPER  FV  SF V +LQHIQF LNHF+A+VYVG PTG+DWFEKQ +GT+DISC S+
Sbjct: 304 LPNWPERFFFVFTSFTVTALQHIQFTLNHFAADVYVGPPTGSDWFEKQAAGTIDISCRSY 363

Query: 362 MDWFFGGLQFQLEHHLFPRMPRCQLRKISPIVVELCKKHNLPYRSLSFWDANVRTIRTLR 421
           MDWFFGGLQFQLEHHLFPR+PRC LRK+SP+V ELCKKHNLPYRS+S+++ANV TI TL+
Sbjct: 364 MDWFFGGLQFQLEHHLFPRLPRCHLRKVSPVVQELCKKHNLPYRSMSWFEANVLTINTLK 423

Query: 422 TAALQARDLTNPVPKNLLWEAVNTHG 448
           TAA QARD+ NPV KNL+WEA+NTHG
Sbjct: 424 TAAYQARDVANPVVKNLVWEALNTHG 449

BLAST of IVF0009561 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 70.5 bits (171), Expect = 3.9e-12
Identity = 46/121 (38.02%), Postives = 66/121 (54.55%), Query Frame = 0

Query: 6   KYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAF-IAYHP 65
           K  + EE   HNK  D W+ I GKVY+V+ +   HPGGD   L +AG D TD F  A H 
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  GTAWAYLDRLFTGFHLKDFQVSEIS--KDYRKLASEFSKQGLFE--KKGHVIMYSLISVA 122
             A   +++ F G  L +  + EI   K Y+K   + S Q LF+  K+  V+  S+I+++
Sbjct: 66  KDARELMEKYFIG-ELDESSLPEIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSIITIS 125

BLAST of IVF0009561 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 67.8 bits (164), Expect = 2.6e-11
Identity = 33/78 (42.31%), Postives = 45/78 (57.69%), Query Frame = 0

Query: 1  MEVEKKYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAF- 60
          M  ++K ++ EE+ KHNK  D W+ I GKVY+VT +   HPGGD   L+  G D T+ F 
Sbjct: 1  MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61 IAYHPGTAWAYLDRLFTG 78
             H  TA   +D+ F G
Sbjct: 61 DVGHSDTARDMMDKYFIG 78

BLAST of IVF0009561 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 63.5 bits (153), Expect = 4.8e-10
Identity = 32/73 (43.84%), Postives = 39/73 (53.42%), Query Frame = 0

Query: 6  KYITSEELLKHNKPGDLWISIQGKVYNVTEWAKVHPGGDTPFLNLAGHDVTDAF-IAYHP 65
          K  T  E+ +H+   D WI I GKVY+VT++   HPGGD   L   G D TD F    H 
Sbjct: 6  KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65

Query: 66 GTAWAYLDRLFTG 78
           TA A LD  + G
Sbjct: 66 STAKAMLDEYYVG 78

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3EBF71.9e-20873.77Delta(8)-fatty-acid desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=SLD2 PE=1 SV... [more]
Q9ZRP73.3e-20573.09Delta(8)-fatty-acid desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=SLD1 PE=1 SV... [more]
Q434695.2e-20372.69Delta(8)-fatty-acid desaturase OS=Helianthus annuus OX=4232 GN=sld1 PE=1 SV=1[more]
Q9FR826.8e-20374.50Delta(8)-fatty-acid desaturase OS=Borago officinalis OX=13363 GN=sld1 PE=1 SV=1[more]
O043532.4e-17963.88Acyl-lipid (9-3)-desaturase OS=Borago officinalis OX=13363 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CS554.8e-268100.00Delta(8)-fatty-acid desaturase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C7C04.8e-268100.00delta(8)-fatty-acid desaturase 2 OS=Cucumis melo OX=3656 GN=LOC103497683 PE=4 SV... [more]
A0A0A0KA293.2e-26498.21Delta8-sphingolipid desaturase OS=Cucumis sativus OX=3659 GN=Csa_6G006850 PE=4 S... [more]
A0A6J1KW797.2e-25694.63delta(8)-fatty-acid desaturase 2-like OS=Cucurbita maxima OX=3661 GN=LOC11149926... [more]
A0A6J1H4851.6e-25594.41delta(8)-fatty-acid desaturase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111460... [more]
Match NameE-valueIdentityDescription
XP_008458161.10.0100.00PREDICTED: delta(8)-fatty-acid desaturase 2 [Cucumis melo] >TYK14028.1 delta(8)-... [more]
XP_031743128.10.098.21delta(8)-fatty-acid desaturase 2 [Cucumis sativus] >ANN11751.1 delta8-sphingolip... [more]
XP_038874611.10.096.87delta(8)-fatty-acid desaturase 2 [Benincasa hispida][more]
XP_023006577.10.094.63delta(8)-fatty-acid desaturase 2-like [Cucurbita maxima] >XP_023006578.1 delta(8... [more]
XP_022959210.10.094.41delta(8)-fatty-acid desaturase 2-like [Cucurbita moschata] >XP_022959211.1 delta... [more]
Match NameE-valueIdentityDescription
AT2G46210.11.3e-20973.77Fatty acid/sphingolipid desaturase [more]
AT3G61580.12.3e-20673.09Fatty acid/sphingolipid desaturase [more]
AT1G26340.13.9e-1238.02cytochrome B5 isoform A [more]
AT5G53560.12.6e-1142.31cytochrome B5 isoform E [more]
AT5G48810.14.8e-1043.84cytochrome B5 isoform D [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 8..80
e-value: 5.2E-19
score: 79.2
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 9..77
e-value: 1.1E-20
score: 73.5
IPR001199Cytochrome b5-like heme/steroid binding domainPROSITEPS50255CYTOCHROME_B5_2coord: 5..89
score: 21.954365
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 1..78
e-value: 9.7E-21
score: 75.4
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 5..86
IPR005804Fatty acid desaturase domainPFAMPF00487FA_desaturasecoord: 137..406
e-value: 2.5E-35
score: 122.6
IPR012171Fatty acid desaturasePIRSFPIRSF015921FA_sphingolip_descoord: 1..434
e-value: 2.9E-128
score: 425.9
NoneNo IPR availablePANTHERPTHR19353FATTY ACID DESATURASE 2coord: 1..446
NoneNo IPR availablePANTHERPTHR19353:SF28DELTA(8)-FATTY-ACID DESATURASE 2coord: 1..446
NoneNo IPR availableCDDcd03506Delta6-FADS-likecoord: 140..402
e-value: 4.94138E-63
score: 200.946

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009561.1IVF0009561.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016717 oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
molecular_function GO:0016491 oxidoreductase activity