IVF0009556 (gene) Melon (IVF77) v1

Overview


Name	IVF0009556
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	kunitz trypsin inhibitor 1-like
Location	chr05: 23819959 .. 23821037 (+)
RNA-Seq Expression	IVF0009556
Synteny	IVF0009556

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAACAATTGTGATAAAGCTAGACTTTGGTCATTTTCATCTCCTCCATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATATCATTATTTTTCAAACAAAAACTAAATCACTCTTGTTGAAGAATCAAAAGATGATGAGGTTCCATAAGTCACTATTTTCCATCTTTAGCTTGCTATGCTTCTTCATGGCCATACCCTCTACTGCCGGCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGCGGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGTGATCAGTGCCCATTTTATGTCGGGCAAGTACCACTTCTTTCTCAAGAAACAGGTATGTATTAAATCTATTACTAAACTAAATTGATTCAGTAAGTTATATGATATTTGTTAGAGTGTTGATAGACAATATTAAATGTGAACCTATACTCGCTCACTATTATTTAAGCCGATAGATTACGATATATTGAATTCTTAACAATAGGTTTCGCGGTCTCCCTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGTTTGTGTTTCAAGTGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGCAGATCCAAAGACAGGAAGGAGGTTTGTGAAGCTCGGATACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTGCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGAGTGAGGTTTTTGGCTTTGGATGGAACTGCATTTCCTTTTGAGTTTGTGAGAGTTGATCCTGTAGAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACAAACTCAATTAAAATTAAATAAAACTCTTTGTAAGGACTTAGTATTATGTGTGTTTATTGGGTGTTGTATATGATTAATATATATAATGTGATTTTGGTAGGTGGA

mRNA sequence

TAACAATTGTGATAAAGCTAGACTTTGGTCATTTTCATCTCCTCCATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATATCATTATTTTTCAAACAAAAACTAAATCACTCTTGTTGAAGAATCAAAAGATGATGAGGTTCCATAAGTCACTATTTTCCATCTTTAGCTTGCTATGCTTCTTCATGGCCATACCCTCTACTGCCGGCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGCGGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGTGATCAGTGCCCATTTTATGTCGGGCAAGTACCACTTCTTTCTCAAGAAACAGGTTTCGCGGTCTCCCTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGTTTGTGTTTCAAGTGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGCAGATCCAAAGACAGGAAGGAGGTTTGTGAAGCTCGGATACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTGCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGAGTGAGGTTTTTGGCTTTGGATGGAACTGCATTTCCTTTTGAGTTTGTGAGAGTTGATCCTGTAGAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAATGTGTAAATTTGTTGTAATTGTATACACAAACTCAATTAAAATTAAATAAAACTCTTTGTAAGGACTTAGTATTATGTGTGTTTATTGGGTGTTGTATATGATTAATATATATAATGTGATTTTGGTAGGTGGA

Coding sequence (CDS)

ATGTTGGCTATAAAATGGCACTACACATTTGCATCCCAAAACCATATCATTATTTTTCAAACAAAAACTAAATCACTCTTGTTGAAGAATCAAAAGATGATGAGGTTCCATAAGTCACTATTTTCCATCTTTAGCTTGCTATGCTTCTTCATGGCCATACCCTCTACTGCCGGCCAATTCGATGGTCCTCCGGTGCTCGACACCGAAGGCCGACCTCTGCAGCGCGATGCGGAGTATTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTTACCTTAATTACCCGAAAAGGTGATCAGTGCCCATTTTATGTCGGGCAAGTACCACTTCTTTCTCAAGAAACAGGTTTCGCGGTCTCCCTCGCACCTTATCGAGAGGGTGAAGATACGATCAGAGAAGGCAGAGATTTGAAGTTTGTGTTTCAAGTGTTCACCATTTGCATAACGGGGACTCAATGGAAAGTGGGGGAGGCAGATCCAAAGACAGGAAGGAGGTTTGTGAAGCTCGGATACGACAACACTGCCACCGGATACTTTAGGATAGACAAGAGCGACCTCGGAGTTTATAATATAGGATGGTGTCCCTCGGATGTGCCCATTAAAGGGAGGCCAAGGTGTGGAAGTGCTGGAATTTTGATTGAGAAAGGAGTGAGGTTTTTGGCTTTGGATGGAACTGCATTTCCTTTTGAGTTTGTGAGAGTTGATCCTGTAGAAGAGCTTGGTTCTATCCTGCAAGAGAAAAAATAA

Protein sequence

MLAIKWHYTFASQNHIIIFQTKTKSLLLKNQKMMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEKK

Homology

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BLAST of IVF0009556 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.1e-37
Identity = 87/200 (43.50%), Postives = 123/200 (61.50%), Query Frame = 0


Query: 41  FSIFSLLCFFMAIPSTA-GQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKG 100
           F+IF L   ++ + +T+  QF    V+DT G P++ D +YFI+PAIT   G+LTL+TR  
Sbjct: 6   FTIFILANVWLLVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR-- 65

Query: 101 DQCPFYVGQVPLLSQETGFAVSLAPY--REGEDTIREGRDLKFVFQVFTICITGTQWKVG 160
           + CPF VG  P   Q  GFAV L+P+     ED +R GRDL+ +FQ  T C   T+W++G
Sbjct: 66  NSCPFNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLG 125

Query: 161 EADPKTGRRFVKLGYDNTATG----YFRIDKS-DLGVYNIGWCPSDVPIKGRPRCGSAGI 220
           E D  TGRRF+  G D++  G    +FRI ++   G++NI WCP++V    +  CG+ GI
Sbjct: 126 ERDATTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGI 185

Query: 221 LIEKGVRFLALDGTAFPFEF 233
           + E G   LALDG+A P  F
Sbjct: 186 VRENGKILLALDGSALPVAF 197

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BLAST of IVF0009556 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 77/206 (37.38%), Postives = 111/206 (53.88%), Query Frame = 0


Query: 34  MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 93
           MR       I + +C F+   +T  QF    VLDT G P++ D EYFI+P IT+  G  T
Sbjct: 3   MRLSIRTLIILAHVCLFIT-TTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRST 62

Query: 94  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPY--REGEDTIREGRDLKFVFQVFTICITG 153
           +++R  + CP +VG + L     G  V   P+      D +R  RDL+  FQ  + C+  
Sbjct: 63  MVSR-NESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQS 122

Query: 154 TQWKVGEADPKTGRRFVKLGYDNTATG----YFRIDKSDL-GVYNIGWCPSDVPIKGRPR 213
           T+W++GE D K+GRR +  G D+   G    +FRI ++ L G+YNI WCP++V    +  
Sbjct: 123 TEWRLGEKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFE 182

Query: 214 CGSAGILIEKGVRFLALDGTAFPFEF 233
           CG+  +L E G   LALDG   P  F
Sbjct: 183 CGTVDMLNENGKILLALDGGPLPLVF 201

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BLAST of IVF0009556 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-30
Identity = 76/198 (38.38%), Postives = 108/198 (54.55%), Query Frame = 0


Query: 42  SIFSLLCFFMAIPSTA-GQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGD 101
           +IF L   ++ + +T+  QF    V+DT G P++ D EYFI+PAIT   G  TL+T  G 
Sbjct: 7   TIFILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG- 66

Query: 102 QCPFYVGQVPLLSQETGFAVSLAPY--REGEDTIREGRDLKFVFQVFTICITGTQWKVGE 161
            CP +VG +       G AV   P+  +  +D +R  RDL+  F   T C   T W++GE
Sbjct: 67  PCPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGE 126

Query: 162 ADPKTGRRFVKLGYDNTA---TGYFRIDKSDL-GVYNIGWCPSDVPIKGRPRCGSAGILI 221
            D  +GRR +  G DN A     +FRI ++   G YNI WCP++     + +CG+ G++ 
Sbjct: 127 KDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIR 186

Query: 222 EKGVRFLALDGTAFPFEF 233
           E G   LALDG A P  F
Sbjct: 187 ENGKNLLALDGDALPVVF 198

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BLAST of IVF0009556 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.4e-16
Identity = 58/183 (31.69%), Postives = 84/183 (45.90%), Query Frame = 0


Query: 65  VLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQETGFAVSLAP 124
           V+D +G  +  ++ Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHES-YYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 125 YREGEDTIREGRDLKFVFQV-FTICITGTQWKVGEADPKTGRRFVKLG------YDNTAT 184
           +R     + E ++L     V  TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 185 GYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILI-EKGVRFLALDGTAFPFEFVRVD 240
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

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BLAST of IVF0009556 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 6.0e-15
Identity = 51/143 (35.66%), Postives = 73/143 (51.05%), Query Frame = 0


Query: 49  FFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQ 108
           F+ A PS A   D PPV DT+G  L+ DA Y++  A     G LT+    G  CP +V Q
Sbjct: 14  FWPAPPSRAA--DPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQ 73

Query: 109 VPLLSQETGFAVSLAPY--REGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRR 168
            P   Q  GF V + PY     +  IR   D++  F+ +T C+  T+W + +++   GRR
Sbjct: 74  DP-NGQHDGFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRR 133

Query: 169 FVKLG--YDNTATG---YFRIDK 185
            V  G   D + +G    FRI+K
Sbjct: 134 HVITGPVKDPSPSGRENAFRIEK 152

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BLAST of IVF0009556 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.8e-123
Identity = 215/216 (99.54%), Postives = 215/216 (99.54%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEK 249
           LIEKGVRFLALDG AFPFEFVRVDPVEELGSILQEK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of IVF0009556 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 9.0e-123
Identity = 213/217 (98.16%), Postives = 214/217 (98.62%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSLF+IFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPL SQETGF VSLAPYREGEDTIREGRDLKFVFQVFTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEKK 250
           LIEKGVRFLALDG AFPFEFVRVDPVEELGSILQEKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of IVF0009556 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.4e-110
Identity = 195/217 (89.86%), Postives = 202/217 (93.09%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEKK 250
           LIEKGVRFLALDG AFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of IVF0009556 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 7.7e-82
Identity = 149/201 (74.13%), Postives = 165/201 (82.09%), Query Frame = 0


Query: 34  MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 93
           M   KSL + FS LC  MA+ S A     PPVLDTEGRPLQ + EYFIKPA+TDVAGNLT
Sbjct: 1   MMLCKSLVA-FSYLCLVMAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLT 60

Query: 94  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 153
           L+TRKGDQCPF+VGQVPL SQE GF V+  PY  GEDTIREGRDLK VFQ F+ICIT T 
Sbjct: 61  LLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTA 120

Query: 154 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 213
           W+VGE DP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGIL
Sbjct: 121 WRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGIL 180

Query: 214 IEKGVRFLALDGTAFPFEFVR 235
           IEKG+R LALDG+AFPFEFVR
Sbjct: 181 IEKGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of IVF0009556 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.6e-79
Identity = 147/201 (73.13%), Postives = 162/201 (80.60%), Query Frame = 0


Query: 34  MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 93
           M   KSL + FS LC  MA  S A     PPVLD EGRPL+ + EYFIKPAITDVAGNLT
Sbjct: 1   MMLCKSLVA-FSYLCLVMAASSIA---QSPPVLDIEGRPLEINVEYFIKPAITDVAGNLT 60

Query: 94  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 153
           L+TRKGDQCPF+VGQVPL SQE GF V+  PY   ED IREGRDLK VFQ F+ICIT T 
Sbjct: 61  LLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTA 120

Query: 154 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 213
           W+VGEADP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGIL
Sbjct: 121 WRVGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGIL 180

Query: 214 IEKGVRFLALDGTAFPFEFVR 235
           IEKG+R LALDG+AFPFEFVR
Sbjct: 181 IEKGLRLLALDGSAFPFEFVR 197

[Close]

BLAST of IVF0009556 vs. NCBI nr
Match: XP_008455014.1 (PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo])

HSP 1 Score: 442 bits (1136), Expect = 5.71e-156
Identity = 215/216 (99.54%), Postives = 215/216 (99.54%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEK 248
           LIEKGVRFLALDG AFPFEFVRVDPVEELGSILQEK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEK 216

[Close]

BLAST of IVF0009556 vs. NCBI nr
Match: KAA0063688.1 (endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >TYK18339.1 endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 439 bits (1130), Expect = 4.87e-155
Identity = 213/217 (98.16%), Postives = 214/217 (98.62%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSLF+IFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPL SQETGF VSLAPYREGEDTIREGRDLKFVFQVFTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI
Sbjct: 121 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEKK 249
           LIEKGVRFLALDG AFPFEFVRVDPVEELGSILQEKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDPVEELGSILQEKK 217

[Close]

BLAST of IVF0009556 vs. NCBI nr
Match: XP_004139193.1 (endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus])

HSP 1 Score: 399 bits (1025), Expect = 4.72e-139
Identity = 195/217 (89.86%), Postives = 202/217 (93.09%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MMRFHKSL ++FS LCFFMAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMRFHKSLIALFSWLCFFMAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPLLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGT
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGT 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGE DPK+GRRFVKLG DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGI
Sbjct: 121 QWKVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPVEELGSILQEKK 249
           LIEKGVRFLALDG AFPFEFVRVD  EELGSIL+EKK
Sbjct: 181 LIEKGVRFLALDGPAFPFEFVRVDTGEELGSILKEKK 216

[Close]

BLAST of IVF0009556 vs. NCBI nr
Match: KAE8651587.1 (hypothetical protein Csa_023465 [Cucumis sativus])

HSP 1 Score: 372 bits (956), Expect = 8.10e-129
Identity = 181/199 (90.95%), Postives = 186/199 (93.47%), Query Frame = 0


Query: 51  MAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 110
           MAIPSTA QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP
Sbjct: 1   MAIPSTA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVP 60

Query: 111 LLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVGEADPKTGRRFVKL 170
           LLSQE GF V+L PY EG+DTIREGRDLKFVFQ FTICITGTQWKVGE DPK+GRRFVKL
Sbjct: 61  LLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGEEDPKSGRRFVKL 120

Query: 171 GYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKGVRFLALDGTAFPF 230
           G DNTATGYFRIDKS+LGVYNIGWCPSDVP KGRPRCGSAGILIEKGVRFLALDG AFPF
Sbjct: 121 GNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGVRFLALDGPAFPF 180

Query: 231 EFVRVDPVEELGSILQEKK 249
           EFVRVD  EELGSIL+EKK
Sbjct: 181 EFVRVDTGEELGSILKEKK 198

[Close]

BLAST of IVF0009556 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 365 bits (938), Expect = 8.47e-126
Identity = 184/218 (84.40%), Postives = 194/218 (88.99%), Query Frame = 0


Query: 33  MMRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNL 92
           MM FHKSL +IFS LCFFMAI S A QFDGPPVLDTEGRPLQRD EYFIKPAITDVAGNL
Sbjct: 1   MMMFHKSL-AIFSSLCFFMAISSIA-QFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNL 60

Query: 93  TLITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGT 152
           TLITRKGDQCPFYVGQVPLLSQ+ GF V+  PY  GEDTIREGRDLK VFQ FTICIT T
Sbjct: 61  TLITRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITST 120

Query: 153 QWKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGI 212
           QWKVGEADP TGRRFVKLG D+TATGYFRIDKSDLGVYNIGWCPSDVPI+GRPRCGSAGI
Sbjct: 121 QWKVGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGI 180

Query: 213 LIEKGVRFLALDGTAFPFEFVRVDPV-EELGSILQEKK 249
           LIEKG+R LALDG+AFPFEFVRV+   ++L SI QEKK
Sbjct: 181 LIEKGLRLLALDGSAFPFEFVRVEIAGKKLDSIFQEKK 216

[Close]

BLAST of IVF0009556 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 86.3 bits (212), Expect = 3.9e-17
Identity = 58/183 (31.69%), Postives = 84/183 (45.90%), Query Frame = 0


Query: 65  VLDTEGRPLQRDAEYFIKPAITDVAGNLTLITRKGDQCPFYVGQVPLLSQETGFAVSLAP 124
           V+D +G  +  ++ Y++ P I    G LTL  R G  CP+ + Q      E G  V  + 
Sbjct: 30  VVDIDGNAMFHES-YYVLPVIRGRGGGLTLAGRGGQPCPYDIVQESSEVDE-GIPVKFSN 89

Query: 125 YREGEDTIREGRDLKFVFQV-FTICITGTQWKVGEADPKTGRRFVKLG------YDNTAT 184
           +R     + E ++L     V  TICI  T W+VGE D +  + FV  G        ++  
Sbjct: 90  WRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYFVVAGPKPEGFGQDSLK 149

Query: 185 GYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILI-EKGVRFLALDGTAFPFEFVRVD 240
            +F+I+KS    Y   +CP      G P+C   GI I E GVR LAL    F   F + +
Sbjct: 150 SFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRRLALSDKPFLVMFKKAN 209

The following BLAST results are available for this feature:

BLAST of IVF0009556 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	1.1e-37	43.50	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	1.2e-31	37.38	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	1.3e-30	38.38	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
Q8RXD5	5.4e-16	31.69	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P07596	6.0e-15	35.66	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of IVF0009556 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3C0M7	1.8e-123	99.54	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5A7V7Y2	9.0e-123	98.16	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]
A0A0A0LGB4	1.4e-110	89.86	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A6J1FD70	7.7e-82	74.13	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	1.6e-79	73.13	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]

BLAST of IVF0009556 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455014.1	5.71e-156	99.54	PREDICTED: endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo]	[more]
KAA0063688.1	4.87e-155	98.16	endogenous alpha-amylase/subtilisin inhibitor-like [Cucumis melo var. makuwa] >T...	[more]
XP_004139193.1	4.72e-139	89.86	endogenous alpha-amylase/subtilisin inhibitor [Cucumis sativus]	[more]
KAE8651587.1	8.10e-129	90.95	hypothetical protein Csa_023465 [Cucumis sativus]	[more]
XP_038888505.1	8.47e-126	84.40	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]

BLAST of IVF0009556 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	3.9e-17	31.69	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 64..236 e-value: 4.3E-25 score: 99.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 65..233 e-value: 8.8E-36 score: 123.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 38..235
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 65..81
None	No IPR available	GENE3D	2.80.10.50		coord: 62..236 e-value: 1.9E-38 score: 133.9
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 38..235
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 63..235

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0009556.1	IVF0009556.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity