IVF0009155 (gene) Melon (IVF77) v1

Overview
NameIVF0009155
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionProtein NUCLEAR FUSION DEFECTIVE 4-like
Locationchr04: 30524814 .. 30526538 (-)
RNA-Seq ExpressionIVF0009155
SyntenyIVF0009155
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCTCAACCTCAAATCCCACCTCCGCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCCACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAATGTCGGCGTCATCAGCGGTCTCATCAACGAGGTCGCTCCCGCCTGGGTGGTTCTTCTGATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTACATCTGCCTCGGAGCTAATTCTCAGACCTTTGCAAATACAGGGGCTCTTGTAACCTGCGTCAAAAATTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGCGGCGCCATTTTGTCCCAACTTTTCCATGCCTTTTATGGCAATGATTCCAAATCTCTTATCCTCTTAATCGGTTGGCTTCCCGCTGCCGTCTCTGTTGTTTTCCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCAGCCCAACGAGGTTAACGTTTTTTACCATATCCTTTACATTTCTCTCAGCCTTGCTGGGACTCTAATGGTCTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCTAACCCAATTTTACAATTAGAGTTAGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGCCACTAGCGCCGTCGAGCCCTCCATCGGACTCCTGCTTTAAAAACATGTTTAAACCTCCAAACAGAGGAGAAGACTACACTATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGAGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCATTGCGGTACCCATCTCACAGTACCACCACATTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTCGTGACGCTTATTCTGTCCTGCATCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTACAATATGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCATGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCGGCGGGGAGACTGAGAAAAAAAGGGGAGGATTTGAGTTGCTTGGGAGTGGAATGTTACAGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCGCTTGTTTCGCTGATTTTGGTGGTTCGTACATGGAAGTTTTATAAGGGCGATATTTACCGGAGGTTTAGGGCGGAGGAAGGTGAAGACATCGAAATGAAATGGCCGCTCCGACCAACAGTACGTTGA

mRNA sequence

ATGGCCGCCTCAACCTCAAATCCCACCTCCGCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCCACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAATGTCGGCGTCATCAGCGGTCTCATCAACGAGGTCGCTCCCGCCTGGGTGGTTCTTCTGATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTACATCTGCCTCGGAGCTAATTCTCAGACCTTTGCAAATACAGGGGCTCTTGTAACCTGCGTCAAAAATTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGCGGCGCCATTTTGTCCCAACTTTTCCATGCCTTTTATGGCAATGATTCCAAATCTCTTATCCTCTTAATCGGTTGGCTTCCCGCTGCCGATCTCCGGCAGCCCAACGAGGTTAACGTTTTTTACCATATCCTTTACATTTCTCTCAGCCTTGCTGGGACTCTAATGGTCTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCTAACCCAATTTTACAATTAGAGTTAGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGCCACTAGCGCCGTCGAGCCCTCCATCGGACTCCTGCTTTAAAAACATGTTTAAACCTCCAAACAGAGGAGAAGACTACACTATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGAGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCATTGCGGTACCCATCTCACAGTACCACCACATTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTCGTGACGCTTATTCTGTCCTGCATCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTACAATATGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCATGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCGGCGGGGAGACTGAGAAAAAAAGGGGAGGATTTGAGTTGCTTGGGAGTGGAATGTTACAGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCGCTTGTTTCGCTGATTTTGGTGGTTCGTACATGGAAGTTTTATAAGGGCGATATTTACCGGAGGTTTAGGGCGGAGGAAGGTGAAGACATCGAAATGAAATGGCCGCTCCGACCAACAGTACGTTGA

Coding sequence (CDS)

ATGGCCGCCTCAACCTCAAATCCCACCTCCGCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCTTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCCACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGCTTCTTCAAGGACTTGGGCGGCAATGTCGGCGTCATCAGCGGTCTCATCAACGAGGTCGCTCCCGCCTGGGTGGTTCTTCTGATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAATAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTACATCTGCCTCGGAGCTAATTCTCAGACCTTTGCAAATACAGGGGCTCTTGTAACCTGCGTCAAAAATTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTCGGCTTAAGCGGCGCCATTTTGTCCCAACTTTTCCATGCCTTTTATGGCAATGATTCCAAATCTCTTATCCTCTTAATCGGTTGGCTTCCCGCTGCCGATCTCCGGCAGCCCAACGAGGTTAACGTTTTTTACCATATCCTTTACATTTCTCTCAGCCTTGCTGGGACTCTAATGGTCTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGAAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGGCCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCTAACCCAATTTTACAATTAGAGTTAGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGCCACTAGCGCCGTCGAGCCCTCCATCGGACTCCTGCTTTAAAAACATGTTTAAACCTCCAAACAGAGGAGAAGACTACACTATCCCACAAGCCATTTTCAGTATCGATATGATAATTCTTTTTATCGCCACAATTTGTGGAGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCATTGCGGTACCCATCTCACAGTACCACCACATTCATCTCTCTAGTCAGCATATGGAACTACCTCGGCCGTGTAGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTATTTCTCTTCGTGACGCTTATTCTGTCCTGCATCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCATAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTTTGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTACAATATGGGTGGAGCGGCGAGTCCGATTGGGGCGTATATCATGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCGGCGGGGAGACTGAGAAAAAAAGGGGAGGATTTGAGTTGCTTGGGAGTGGAATGTTACAGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCGCTTGTTTCGCTGATTTTGGTGGTTCGTACATGGAAGTTTTATAAGGGCGATATTTACCGGAGGTTTAGGGCGGAGGAAGGTGAAGACATCGAAATGAAATGGCCGCTCCGACCAACAGTACGTTGA

Protein sequence

MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIGWLPAADLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKGDIYRRFRAEEGEDIEMKWPLRPTVR
Homology
BLAST of IVF0009155 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.7e-29
Identity = 139/547 (25.41%), Postives = 249/547 (45.52%), Query Frame = 0

Query: 19  LFGRWFS---VFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGV 78
           +FGRW     + A+I I +  G  + F+ YSS +KS LG  Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 79  ISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYIC--LGANSQTF 138
            SG+     P  VVL   A M   GY + WL +TN I  P   +  +++C  L   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP---YSLVFLCCLLAGLSICW 156

Query: 139 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIGWLPAA 198
            NT   + C+++FP +R   L L   F G+S A+ S  F+A   + S   +LL   +P  
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLV 216

Query: 199 --------DLRQPN-EVNVFY-------HILYISLSLAG-TLMVLIILQSLLRFQQIQYV 258
                    L +P+ +    Y       H+  I   LA  T   L++  S     ++ ++
Sbjct: 217 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFI 276

Query: 259 GSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPL--APSSP 318
           G+ ++++  L  PL +  R+           P++   L  +     + +   L    +S 
Sbjct: 277 GAVVLLVFPLCAPLLVYARDYFL--------PVINARLNHESSGYVMLNIDELKNQKTSV 336

Query: 319 PSDSCFKNM--FKPPNR---GEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIG 378
            S + +++M   K  N    G++++    I  ++  + +IA  CG    L   +NLGQI 
Sbjct: 337 SSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 396

Query: 379 ESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLL 438
           +SL     ++TT +++ S +++ GR++S    ++  K++++ R  +  + L+ + I   L
Sbjct: 397 QSL---GQNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFL 456

Query: 439 IAF--GVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 498
           +A       +L  ++ +IG   G  +    +I S++FG       +N+     PIG+ + 
Sbjct: 457 LAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLY 516

Query: 499 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 535
              +A  +Y+  A   +          + + C+G +CY K F+     ++ G + SL L 
Sbjct: 517 GY-IAASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLY 562

BLAST of IVF0009155 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 1070.8 bits (2768), Expect = 1.9e-309
Identity = 553/566 (97.70%), Postives = 553/566 (97.70%), Query Frame = 0

Query: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180
           CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 KSLILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240
           KSLILLIGWLPAA             DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360
           PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420
           IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480
           LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 554
           VRTWKFYKGDIYRRFRAEEGEDIEMK
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 566

BLAST of IVF0009155 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 3.0e-283
Identity = 506/566 (89.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAAS+SNPTS+NPFWYQFLFGRWFSV ASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180
           CLYI +GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+ AFYGN+ 
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 KSLILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240
           +SLILLI WLPAA             DLRQPNE+ VFYH LYISL LAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPL IVFREELSVWKSKIA+P+LQLE ASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL +M G ASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 554
           VRTWKFYKGDIYR+FR  EGEDIEMK
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMK 566

BLAST of IVF0009155 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 950.3 bits (2455), Expect = 3.7e-273
Identity = 485/558 (86.92%), Postives = 507/558 (90.86%), Query Frame = 0

Query: 9   TSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68
           TS NPFWY  LFGRWFSVFASILIMSV+GATYMF LYSS IKSSL YDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 69  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGA 128
           LG N+GVISGL+NEVAPAWVVLLIG VMNLFGYTMIWLAVTNRIP PQIWHMCLYIC+GA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIG 188
           NSQTFANT AL+TCVKNFPESRGS+LGL KGFVGLSGAILSQLFHAFYGN+SKSLI LI 
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 189 WLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQY 248
           WLP+A             DLRQPNE+ VFYH+LYISL LAG+LMV IILQ+ LRFQQI Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 249 VGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPP 308
           VGSAIVVIVLLLLPLAIVFREEL +W+SKI NPI QLELASQ PPPP        P +PP
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP--------PPNPP 302

Query: 309 SDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 368
           SDSCFKNMF PPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP
Sbjct: 303 SDSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 362

Query: 369 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 428
           SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP
Sbjct: 363 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 422

Query: 429 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 488
           NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL
Sbjct: 423 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 482

Query: 489 YDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548
           YD EA+RQMEAAGRLRK+GEDLSCLGVECY+KAFLIIT +TV G LVSLILVVRTWKFYK
Sbjct: 483 YDMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYK 542

Query: 549 GDIYRRFRAEEGEDIEMK 554
            DIYRRF+ +EGEDIE+K
Sbjct: 543 DDIYRRFKEKEGEDIEIK 552

BLAST of IVF0009155 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 7.7e-271
Identity = 476/563 (84.55%), Postives = 514/563 (91.30%), Query Frame = 0

Query: 4   STSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSL 183
           IC+GANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+HAFYGN+SKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRF 243
           ILLI WLPAA             DLRQPNE+ VF+HILYISL LAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLA 303
            Q++Y GSAIVVI LLLLPLAIV+REELSVWK+KI +P+ QLELASQQ PPPLTS  P+A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTIPQA+FSIDMIILFIATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  FVT+ILSC+GHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAYI+NVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEAAGR R  GEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRRFRAEEGEDIEMK 554
           WKFYKGDIYR+FR E GE++E++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQ 564

BLAST of IVF0009155 vs. ExPASy TrEMBL
Match: A0A6J1KSH4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498302 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.6e-268
Identity = 473/563 (84.01%), Postives = 512/563 (90.94%), Query Frame = 0

Query: 4   STSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSL 183
           IC+GANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+HAFYGN+SKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRF 243
           ILLI WLPAA             DLRQPNE+ VF+HILYISL LAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLA 303
            Q++Y GSAIVVI LLLLPLAIV+REELSVWK+KI +PI QLELASQ  PPPLTS  P+A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPVA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTI QA+FSIDMIILFIATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  FVT+ILSC+GHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAYI+NVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEAAGR R  GEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRRFRAEEGEDIEMK 554
           WKFYKGDIY++FR + GE++E++
Sbjct: 542 WKFYKGDIYQKFREDSGEEVEVQ 564

BLAST of IVF0009155 vs. NCBI nr
Match: KAA0043983.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1074 bits (2777), Expect = 0.0
Identity = 553/566 (97.70%), Postives = 553/566 (97.70%), Query Frame = 0

Query: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180
           CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 KSLILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240
           KSLILLIGWLPAA             DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360
           PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420
           IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480
           LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 553
           VRTWKFYKGDIYRRFRAEEGEDIEMK
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 566

BLAST of IVF0009155 vs. NCBI nr
Match: XP_038905012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 994 bits (2571), Expect = 0.0
Identity = 509/563 (90.41%), Postives = 525/563 (93.25%), Query Frame = 0

Query: 4   STSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           + SNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 121

Query: 124 ICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSL 183
           IC+GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGN+SKSL
Sbjct: 122 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNNSKSL 181

Query: 184 ILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRF 243
           I LI WLPAA             DLRQPNE+ VFYHILYISL LAG+LMVLIILQ+ LRF
Sbjct: 182 IFLIAWLPAAVSVTFLRFVRIIKDLRQPNELKVFYHILYISLGLAGSLMVLIILQNRLRF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLA 303
           QQIQYVGSAIVVIVLLLLPLAIVFREEL+VWKSKIANPILQLELASQQPP P     PLA
Sbjct: 242 QQIQYVGSAIVVIVLLLLPLAIVFREELNVWKSKIANPILQLELASQQPPRP----PPLA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCFKN FKPPNRGEDYTIPQAIFS+DMIILFIATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFKNTFKPPNRGEDYTIPQAIFSVDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLI 423
           SL YPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GHLLI
Sbjct: 362 SLGYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVR 483
           AFGVPNSLYFSSI++GFCFGAQWPLIFAIISEIFGLKYYATLYN+GGAASPIGAYI+NVR
Sbjct: 422 AFGVPNSLYFSSIVVGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREAQRQMEAAGR R  GEDLSCLGVECYRKAFLIITAATVFG LVSLILVVRT
Sbjct: 482 VAGHLYDREAQRQMEAAGRRRNIGEDLSCLGVECYRKAFLIITAATVFGGLVSLILVVRT 541

Query: 544 WKFYKGDIYRRFRAEEGEDIEMK 553
           WKFYK DIYR+FR EE ED+E+K
Sbjct: 542 WKFYKSDIYRKFREEEAEDVEIK 560

BLAST of IVF0009155 vs. NCBI nr
Match: XP_011651970.1 (uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical protein Csa_001993 [Cucumis sativus])

HSP 1 Score: 987 bits (2551), Expect = 0.0
Identity = 506/566 (89.40%), Postives = 526/566 (92.93%), Query Frame = 0

Query: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAAS+SNPTS+NPFWYQFLFGRWFSV ASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180
           CLYI +GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+ AFYGN+ 
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 KSLILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240
           +SLILLI WLPAA             DLRQPNE+ VFYH LYISL LAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPL IVFREELSVWKSKIA+P+LQLE ASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL +M G ASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRRFRAEEGEDIEMK 553
           VRTWKFYKGDIYR+FR  EGEDIEMK
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMK 566

BLAST of IVF0009155 vs. NCBI nr
Match: KAA0043982.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25158.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 954 bits (2466), Expect = 0.0
Identity = 485/558 (86.92%), Postives = 507/558 (90.86%), Query Frame = 0

Query: 9   TSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68
           TS NPFWY  LFGRWFSVFASILIMSV+GATYMF LYSS IKSSL YDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 69  LGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGA 128
           LG N+GVISGL+NEVAPAWVVLLIG VMNLFGYTMIWLAVTNRIP PQIWHMCLYIC+GA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIG 188
           NSQTFANT AL+TCVKNFPESRGS+LGL KGFVGLSGAILSQLFHAFYGN+SKSLI LI 
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 189 WLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQY 248
           WLP+A             DLRQPNE+ VFYH+LYISL LAG+LMV IILQ+ LRFQQI Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 249 VGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPP 308
           VGSAIVVIVLLLLPLAIVFREEL +W+SKI NPI QLELASQ PPPP        P +PP
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP--------PPNPP 302

Query: 309 SDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 368
           SDSCFKNMF PPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP
Sbjct: 303 SDSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 362

Query: 369 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 428
           SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP
Sbjct: 363 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 422

Query: 429 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 488
           NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL
Sbjct: 423 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 482

Query: 489 YDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548
           YD EA+RQMEAAGRLRK+GEDLSCLGVECY+KAFLIIT +TV G LVSLILVVRTWKFYK
Sbjct: 483 YDMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYK 542

Query: 549 GDIYRRFRAEEGEDIEMK 553
            DIYRRF+ +EGEDIE+K
Sbjct: 543 DDIYRRFKEKEGEDIEIK 552

BLAST of IVF0009155 vs. NCBI nr
Match: KAG6596491.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 946 bits (2445), Expect = 0.0
Identity = 476/563 (84.55%), Postives = 514/563 (91.30%), Query Frame = 0

Query: 4   STSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           + SNP++ +P WYQFL GRWFSVFASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GLINEVAP WVVLLIGAVMNLFGYTMIW+AVT+RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSL 183
           IC+GANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QL+HAFYGN+SKSL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIGWLPAA-------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRF 243
           ILLI WLPAA             DLRQPNE+ VF+HILYISL LAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLA 303
            Q++Y GSAIVVI LLLLPLAIV+REELSVWK+KI +P+ QLELASQQ PPPLTS  PLA
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPLA 301

Query: 304 PSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N FKPPNRGEDYTIPQA+FSIDMIILFIATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLI 423
           SLRYPS STTTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  FVT+ILSC+GHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVR 483
           AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYN+GG ASPIGAYI+NV+
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVK 481

Query: 484 VAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEAAGR R  GEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRRFRAEEGEDIEMK 553
           WKFYKGDIYR+FR E GE++E++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQ 564

BLAST of IVF0009155 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 679.9 bits (1753), Expect = 1.8e-195
Identity = 354/568 (62.32%), Postives = 424/568 (74.65%), Query Frame = 0

Query: 17  QFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           Q L GRWF  F S+LIMS AGATYMF +YS DIK +LGYDQTTLNLLSFFKDLG NVGV+
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 77  SGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGANSQTFANT 136
           +GL+NEV P W +LLIGA++N FGY MIWLAVT RI KPQ+WHMCLYIC+GANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 137 GALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIGWLPA---- 196
           G+LVTCVKNFPESRG VLG+LKG+VGLSGAI++QL+ AFYG D+K LIL+IGWLPA    
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 197 ---------ADLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQYVGSAIVVI 256
                       RQ NE+ VFY+ LYISL LA  LMV+II+  L  F Q ++ GSA VVI
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 257 VLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPPSD------ 316
           VLLLLP+ +V  EE  +WK K      Q+ L    P   +T    L  S    D      
Sbjct: 256 VLLLLPIIVVILEEKKLWKEK------QVALNDPAPINVVTEKPKLDSSEFKDDDGEESK 315

Query: 317 ---------SCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQI 376
                    SC+  +F PP RG+DYTI QA+FS+DM+ILF+ATICGVGGTLTAIDNLGQI
Sbjct: 316 EVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQI 375

Query: 377 GESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHL 436
           G SL YP  S +TF+SLVSIWNY GRVVSG VSE F  KYK PRPL L + L+LSC GHL
Sbjct: 376 GNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHL 435

Query: 437 LIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMN 496
           LIAF VP  LY +S+IIGFCFGAQWPL+FAIISEIFGLKYY+TLYN G  ASPIG+Y++N
Sbjct: 436 LIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLN 495

Query: 497 VRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVV 556
           VRVAG+LYD EA +Q +A G+ R +G+DL+C+G  C++ +F+II A T+FG LVS++LV+
Sbjct: 496 VRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVI 555

BLAST of IVF0009155 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 628.2 bits (1619), Expect = 6.1e-180
Identity = 328/556 (58.99%), Postives = 403/556 (72.48%), Query Frame = 0

Query: 14  FWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F   F  GRWF VFAS LIM+ AGATY+F  YS DIKS+LGYDQTTLNLL FFKDLG NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 74  GVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGANSQTF 133
           GV+SGLI EV P W VL IG+ MN  GY MIWL VT ++ KP++W MCLYIC+GANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIGWLPAA 193
           ANTGALVTCVKNFPESRG +LGLLKG+VGLSGAI +QL+ A YG+DSKSLILLI WLPAA
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 194 -------------DLRQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQYVGSAI 253
                         +RQ NE++VFY  LYIS+ LA  LM + I +  + F +  Y  SA 
Sbjct: 189 VSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASAT 248

Query: 254 VVIVLLLLPLAIVFREELSVW---KSKIANPI-LQLELASQQPPPPLTSTVPLAPSSPPS 313
           +   LL +PL +  ++EL VW   K  I  P  +++E   ++          +      +
Sbjct: 249 ICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKET 308

Query: 314 DSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYPS 373
            SCF  +F PP RGEDYTI QA+ S DMIILF+AT CG+G +LTA+DNLGQIGESL YP+
Sbjct: 309 KSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPN 368

Query: 374 HSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVPN 433
           H+ ++F+SLVSIWNY GRV SGFVSEY   KYK+PRPL + + L+LSC GHLLIAF VP 
Sbjct: 369 HTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPG 428

Query: 434 SLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHLY 493
           S+Y +SI++GF FGAQ PL+FAIISE+FGLKYY+TL+N G  ASP+G+YI+NVRV G LY
Sbjct: 429 SVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLY 488

Query: 494 DREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 553
           D+EA +Q+ A G  RK  +DL+CLG +CY+  FLI+ A T FGALVSL L +RT +FYKG
Sbjct: 489 DKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKG 548

BLAST of IVF0009155 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 1.5e-88
Identity = 203/547 (37.11%), Postives = 311/547 (56.86%), Query Frame = 0

Query: 22  RWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVISGLIN 81
           +W +  ASI I S +GATY FA+YSS +KSS  YDQ+TL+ +S FKD+GG  G+ISG + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 82  EVAPA--------WVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGANSQTF 141
               +        WVV+ +G V    G+  IW +V   I  P +  MCL++ L  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 142 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSK--SLILLIGWLP 201
            NT  +VT  +NF +  G+ +G+++GF+GLSGAIL QL+HA  G +    + ILL+  +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 202 --AADLRQPNEVNVFYHILY-----------ISLSLAGTLMVLIILQSLLRFQQIQYVGS 261
                L  P  V V+  +             IS+ +A  LMV+I ++++L   +   + S
Sbjct: 187 TLVMFLAMP-FVRVYETVTISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFS 246

Query: 262 AIVVIVLLLLPLAIVFR--EELSVWKSKIANPILQLELASQQPPPPLTSTVPLAPSSPPS 321
            I+V++LL  PL +  R   E     S +  P+L             TS +   PSS   
Sbjct: 247 FILVLLLLASPLLVAVRALREKRQTLSSLDGPVLD------------TSALLDPPSS--- 306

Query: 322 DSCFKNMFKPPNR--GEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRY 381
                N+F   +    ED  I +A+ +++  +LF+A +CG+G     ++N+ QIGESLRY
Sbjct: 307 -----NIFPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRY 366

Query: 382 PSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGV 441
            S    + +SL SIWN+LGR  +G+VS+ F  K+  PRP+F+ +TL +  IGH+++A GV
Sbjct: 367 SSVQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGV 426

Query: 442 PNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGH 501
             SLY  S++IG  +G+QW L+  I SEIFG+++  T+Y     A PIG+YI++V+V G+
Sbjct: 427 QGSLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGY 486

Query: 502 LYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFY 542
            YD+ A              +D SC G +C+R +F+I+ +  +FG+LV+ +L  RT KFY
Sbjct: 487 FYDKVASE------------DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFY 520

BLAST of IVF0009155 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 315.1 bits (806), Expect = 1.2e-85
Identity = 193/556 (34.71%), Postives = 311/556 (55.94%), Query Frame = 0

Query: 17  QFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           +F   +W +  ASI I S +GA+Y F +YSS +KSS  YDQ+TL+ +S +KD+G NVG++
Sbjct: 2   EFANTKWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGIL 61

Query: 77  SGLINEVAPA------------WVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYI 136
           SGL      +            W+V+ +G +    GY  IW+A +  IP+P +  MCL++
Sbjct: 62  SGLFYTAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFM 121

Query: 137 CLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLI 196
               + Q F NT  +VT V+NF +  G+ +G++KG++GLSGAIL Q++H F G D ++ I
Sbjct: 122 FFAGHCQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYI 181

Query: 197 LLIGWLP------------------AADLRQPNEVNVFYHILYISLSLAGTLMVLIILQS 256
           LL+  +P                  A D +  N ++       ISL +   LMV+I++++
Sbjct: 182 LLLAVVPSLLILTLMPFVRTYDTVIAGDKKHLNGLSA------ISLIIVTYLMVVILVEN 241

Query: 257 LLRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTST 316
           ++       + S   +++LL  PL +  R +      +  +  L L+        P+T  
Sbjct: 242 IIGMSMPMKICSFTFLLLLLASPLLVAVRAQ-----REEEHRFLSLDF-------PVTER 301

Query: 317 VPLAPSSP-PSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNL 376
             L  S    S S  K++        D  + +AI + +  +LF+A ICG+G  L  I+N+
Sbjct: 302 TTLLDSPKLNSSSDVKDVMT-----NDMNVLEAICTTNFWLLFVAMICGMGSGLATINNI 361

Query: 377 GQIGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCI 436
            Q+GESLRY +    + +SL SIWN+LGR  SG++S+ +   +  PRP+F+ +TL L  I
Sbjct: 362 RQMGESLRYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAI 421

Query: 437 GHLLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAY 496
           GH+++A G+  SLY  S+++G  +G+QW L+  I SEIFG+ +  T++     ASP+G+Y
Sbjct: 422 GHIVMASGLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSY 481

Query: 497 IMNVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLI 542
             +V+V G+LYD+ A              +D SC G  C+R +FLI+ A  + G+LV+L+
Sbjct: 482 FFSVKVIGYLYDKVASE------------DDHSCYGNHCFRTSFLIMAAMALLGSLVALV 522

BLAST of IVF0009155 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 314.3 bits (804), Expect = 2.0e-85
Identity = 200/563 (35.52%), Postives = 303/563 (53.82%), Query Frame = 0

Query: 18  FLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVIS 77
           F+  RW    A++ I S AG  Y+F   S  IKSSL Y+Q  L+ L   KDLG +VG I+
Sbjct: 12  FINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIA 71

Query: 78  GLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLYICLGANSQTFANTG 137
           G ++E+ P W  LL+GAV NL GY  +WL VT R P   +W MC+ I +G N +T+ NTG
Sbjct: 72  GTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTG 131

Query: 138 ALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDSKSLILLIGWLPAADL-- 197
           ALV+ V+NFP+SRG V+G+LKGF GL GAI+SQ++   + ++  SLIL++   PA  +  
Sbjct: 132 ALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVC 191

Query: 198 -------------RQPNEVNVFYHILYISLSLAGTLMVLIILQSLLRFQQIQYVGSAIVV 257
                         +P +   F  I  + L LA  LM ++++Q L+           IV+
Sbjct: 192 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 251

Query: 258 IVLLLLPLAIVFR--------------EELSVWKSKIANPILQL-ELASQQPPPPLTSTV 317
            V+L++P+ +                 EE  V K +   P LQ  +L   +        V
Sbjct: 252 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDV 311

Query: 318 PLAPSS----------------PPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIAT 377
            L P+S                    +   N  + P+RGED+T+ QA+   D  ++F + 
Sbjct: 312 DLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSL 371

Query: 378 ICGVGGTLTAIDNLGQIGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVP 437
           + G G  LT IDNLGQ+ +SL Y   +T   +S++SIWN+LGR+  G+ SE   + Y  P
Sbjct: 372 LLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYP 431

Query: 438 RPLFLFVTLILSCIGHLLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYAT 497
           RP+ + V  ++  +GH+  A+G P ++Y  +++IG  +GA W ++ A  SE+FGLK +  
Sbjct: 432 RPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 491

Query: 498 LYNMGGAASPIGAYIMNVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLI 535
           LYN    A+P G+ + +  +A  +YDREA+RQ  A G +    + L C G  C+    LI
Sbjct: 492 LYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFLTSLI 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4I9E15.7e-2925.41Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TKH41.9e-30997.70Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A0A0LEA43.0e-28389.40Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G74260... [more]
A0A5A7TKY53.7e-27386.92Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1G7M87.7e-27184.55protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1KSH41.6e-26884.01protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498... [more]
Match NameE-valueIdentityDescription
KAA0043983.10.097.70protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 p... [more]
XP_038905012.10.090.41protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_011651970.10.089.40uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical ... [more]
KAA0043982.10.086.92protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25158.1 p... [more]
KAG6596491.10.084.55Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
AT2G39210.11.8e-19562.32Major facilitator superfamily protein [more]
AT2G28120.16.1e-18058.99Major facilitator superfamily protein [more]
AT2G34355.11.5e-8837.11Major facilitator superfamily protein [more]
AT2G34350.11.2e-8534.71Nodulin-like / Major Facilitator Superfamily protein [more]
AT5G14120.12.0e-8535.52Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 22..192
e-value: 9.0E-77
score: 257.9
coord: 196..254
e-value: 9.5E-6
score: 25.3
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 320..533
e-value: 2.4E-12
score: 48.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 9..180
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 193..532
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 9..547
NoneNo IPR availablePANTHERPTHR21576:SF122MFS TRANSPORTERcoord: 9..547
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 21..520
e-value: 1.23423E-116
score: 348.857

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0009155.1IVF0009155.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0099587 inorganic ion import across plasma membrane
biological_process GO:0002239 response to oomycetes
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0022857 transmembrane transporter activity