IVF0009041 (gene) Melon (IVF77) v1

Overview


Name	IVF0009041
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	sirohydrochlorin ferrochelatase, chloroplastic
Location	chr09: 3852876 .. 3856064 (-)
RNA-Seq Expression	IVF0009041
Synteny	IVF0009041

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAGAGAAGACTAGAAATGTAAAGAAAGGGATGGAAAGCCAAAAACAAGTGGGACATGTCATGTGAATGGGAATGAGGTTGGAAATGGGGAACCAAAAAGTTTGGTTATGAATCCTTGAAAGACACTCTTTTTAAATTAGAAAATAATATTTTCATGTGCAATTTTATAAGATAATTAAAGGGTTAGTTTAGTAGTATTTTAAAATCCAAGCAAACCCCAACTACTCATTGTGTTGGTATCTTTGGAAGTTTATATTTCTATGTCAAAACTTATAATGATGTTCTTTTTCTTTTTTCTTTCTTTTTTTTTTTTTTTGAGGGATTTTTAGTTTATTACATTTTTATTTTATATATGATAAAACCATTTTAAATGGAGGAAGGAAAAAAAACATGGGTAGAAATGGAGAATAAACAAAATTTGAATTTTTTTTTTCATGGAATATGAATATTTTAATTGGTCAAGCTTTTAGATCTTCTTTTTTATAATTAATATAGCTAAATCAACTAAAATATCTACCTACATAACCAAATCTTACCCTTCTTAAGTTTGGGTTTAAATATTTTATAATTCTTATGTAATATTTTAAAAAGTCAAAAGCCAAAAAAATAAAAATTCATCTTCCTCTTGTGTTTGATTGATGTAGAAATCATCATAATAGATCAAAAAAATCAATTGGCCAAAATCAATCAAAACGAAGACGATATGTGGCGATAAAGAAAGATTTGGACGGTCTTGATTATGGAAAAGTTCAAACCAACAACATTTAAAAAAATTGAAAAGTTTAAATCGATCCATTTGTACTATTTCATGATTGGAGTCATTTAAATGTTTACTAAATCGATCATAATCAATTCAGCGGCGGTTTAGCGAAGAAACCAACTCTATACTTACCCTTTGAAATTGGTTATTGGGTTGATTTATAAAAATCAAGATCACTTTTACAATTTTCCAATAAAAAGGAACAATTTAGTAAAACCCACCACTTTCATTTTAGGCCAATGCAATTGAATTTACCTACGACGTCGTTTTTGATTATCAGGTTCAAGTGCTCCCTCTTCCCTTGGCGAAGGATCAGAACCCATGTCTTCGTTATCCGTCACTCCTCAAATTTCACCGAAGAGGTTTCATAATCTCCTAAACCCTCATTTTCTTTTCAATTTTTTCTTGATTTTTTTCCTCTCTAATGGCCGTCCATTTTCCCATTGAAGTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTTTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAGTAAGTTAGGGTTTTAGAATTTGGAATTTTGGGTGGGTGAAGTTTTGAAGTTATTCGTTGGATAATTCTCTTGGGGTGATGAACTTTTTTGGAAATTTTCAGATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGGTATGTTCTTTTCAGCTGTTTCTTGTGCACAAAGCTACTACTTTCATTCATGAAGCAACATTATTTGCTTTTGTAGTTGGTTTTTGGAAATCAATGTTACATTTTAGTTAGGGCTGCTTGGGTTGTAATGAAATGAGTGTGTAATTTAATGGGTCCAAAACCCGTGTTTGGATTTAGCCTTTCTGCCCGATTTGTAATCCCAAACTCATGAGTTTCTTTCTGCAGTTTTTAATCTAACCCTCTTTTCTACGATTTTCGTGCTTGATGATTCTATTTCGCATCTCAATGCTTCTCAATTCTTATTGCATTCTAGCTCTCCAAACATTTAGTTAAAAGTATTTTGGTCCCTGTAATTATAGATTTAGTTCATTTTAGTTCATGTAAGTAAATGTATATTTTGGTCCCATAAGTGATATTTTGAAAGTATTGGGATCGAAATGAACATTTTGAAAATATAACGACCAATTGTAAGGACCAAACTAACATTTTAAACCTTTTGTTTGGTTCAACAATGGTGGTGCTAACCACTTCTTTTTGGTATATGTGTAGACCAATTGAACTATGTGTTAAATTATTAACATTTGATCGATCAATTAAGACTGAAAGCAAACCATGATGAAGTAAACTCAACATCACGATTGGATATTTTGATTTTCGAAAAAATGTACAGTTTTGTGGATCTTTTTTCTACACTCTGAAGTACAGCATCTCTAGACTTCAAATTAACATAAGACCCAATGAATAGAAAAGCAAAATAAATACTGATACCATTCAAAATGAATACTGATTTGGAACAATTCTATGTCATCGTACTGTCAGTAGTTTCTCTGTCTTTATTCCTGTCTGTCTCCTTTTGTTGCATTCAACACATGATAGAGTAATAGTCGCAGCCATTTCCAATGTACTTAATGGTCTCATTACATTTTTCTGAAACAACATAGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGTATGTAACTCACTGTAGGACACTTGATTTGATGAAATGAACTGGTGGATTGATGATAGCAATGAGTGAAACTTTGCAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAACTTGTGGTAGGTATTCCTAACCTATATTTTTAATACGAATAGGAATATTAATAACAATTTCCCCAAGGAAAACAATTTAATTGTTAATCTAAATAAATTTTATAAATTTCGTGACTTCCATAAATATCTGTCGATATGTCCGTTTTTTCGATGTGTCCGTAAAATTGAAATCTCTATATATCGATATTGACATTGTCAATTCCCAGATGGAAGAATGAATGTCATAAATGATAAAAGAAAAGGACTATCTTTTAGTATTAAACTATGAGGTGGTTCTGTTGACAGGATGTTGTGAACGATAGAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAG

mRNA sequence

ATGAAAGAGAAGACTAGAAATGTAAAGAAAGGGATGGAAAGCCAAAAACAAGTGGGACATGTCATTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTTTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAACTTAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAG

Coding sequence (CDS)

ATGAAAGAGAAGACTAGAAATGTAAAGAAAGGGATGGAAAGCCAAAAACAAGTGGGACATGTCATTCCTTCACCAGTGGAAACTAGAAGCCCATACACAATCTGCGCGTTTCAGAGATCTCCAATTCCTATCGGACGCCTCTCCAAATTCAGAAATTTCACCTCGCGGGTCCGCATGAGTATTGATAATGGAAGCTCTAATCGGATTGGTGCCGGAGATGCTGTGATTATCGTCGACCACGGCTCCCGTCGCCGGGAATCTAATCTCATGCTGAATGAGTTTGTTGCTATGTTTAGGGATAAAACTGGTTATCCCATTGTGGAGCCTGCTCACATGGAGCTGGCCGAACCATCGATAAGAGACTCATTCACATCATGTGTTGAACAAGGGGCCAAACGTATCATCGTCAGCCCGTTCTTTCTTTTCCCTGGTCGACATTGGCAACAGGATATTCCTTCCTTAACTGCTGAAGCAGCAAAGGATCACCCAGGAATATCGTATATAATCACCGCTCCTCTCGGTCTTCACGAAAACTTAATAAATCACTGCTTAAGCCATGCTGCTGGAGTGGCAGACGAATGTGAAGTTTGTGCTGGCACGGGCAAATGTCGATTTAACTAG

Protein sequence

MKEKTRNVKKGMESQKQVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENLINHCLSHAAGVADECEVCAGTGKCRFN

Homology

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BLAST of IVF0009041 vs. ExPASy Swiss-Prot
Match: Q84JH7 (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=SIRB PE=1 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.3e-57
Identity = 105/145 (72.41%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 67  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSC 126
           N IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 127 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL------- 186
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L       
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 187 INHCLSHAAGVADECEVCAGTGKCR 205
           I HCLSH  G ADEC VCAGT KC+
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

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BLAST of IVF0009041 vs. ExPASy Swiss-Prot
Match: P61817 (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 4.7e-13
Identity = 36/109 (33.03%), Postives = 55/109 (50.46%), Query Frame = 0


Query: 73  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAK 132
           DAV+ V HGSR +E       F+   +     PI E   +ELA P+I   F +C+EQGA 
Sbjct: 13  DAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELASPTIEQGFEACIEQGAT 72

Query: 133 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENLIN 182
           RI + P  L    H + DIP    +  + +P +  +   P G+ E +++
Sbjct: 73  RIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVDERIVD 121

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BLAST of IVF0009041 vs. ExPASy Swiss-Prot
Match: Q58380 (Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=cbiX PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.1e-11
Identity = 36/90 (40.00%), Postives = 52/90 (57.78%), Query Frame = 0


Query: 73  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAK 132
           +A+++V HGSR   S  +L +     +++  +PIVE   ME +EP+I  +    +EQGAK
Sbjct: 2   EALVLVGHGSRLPYSKELLVKLAEKVKERNLFPIVEIGLMEFSEPTIPQAVKKAIEQGAK 61

Query: 133 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDH 163
           RIIV P FL  G H  +DIP L      +H
Sbjct: 62  RIIVVPVFLAHGIHTTRDIPRLLGLIEDNH 91

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BLAST of IVF0009041 vs. ExPASy Swiss-Prot
Match: P61819 (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=cbiX PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.6e-11
Identity = 33/92 (35.87%), Postives = 51/92 (55.43%), Query Frame = 0


Query: 73  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAK 132
           +A+++V HGSR   S  ++ E     + +  Y IVE   ME  EP+I  +    ++ GAK
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 133 RIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPG 165
           ++IV+P FL PG H ++DIP +      D  G
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEG 93

[Close]

BLAST of IVF0009041 vs. ExPASy Swiss-Prot
Match: A6UWT5 (Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-1280 / DSM 17508 / OCM 812 / Nankai-3) OX=419665 GN=cbiX PE=3 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-09
Identity = 29/82 (35.37%), Postives = 48/82 (58.54%), Query Frame = 0


Query: 73  DAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAK 132
           +A++++ HGSR   S  ++ +     ++K  Y IVE   ME  EP+I  +    + +GAK
Sbjct: 2   EALVLLGHGSRLPYSKEIVGKVAEKIKEKNIYDIVEIGMMEFNEPTIPQTINKVIAEGAK 61

Query: 133 RIIVSPFFLFPGRHWQQDIPSL 155
           +II+ P FL  G H ++DIP +
Sbjct: 62  KIIIVPVFLAHGNHTKRDIPQI 83

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BLAST of IVF0009041 vs. ExPASy TrEMBL
Match: A0A5D3BY55 (Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold374G00520 PE=4 SV=1)

HSP 1 Score: 375.2 bits (962), Expect = 1.8e-100
Identity = 184/197 (93.40%), Postives = 185/197 (93.91%), Query Frame = 0


Query: 17  QVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 76
           Q+    PSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI
Sbjct: 9   QISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 68

Query: 77  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 136
           IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV
Sbjct: 69  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 128

Query: 137 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAG 196
           SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       INHCLSHAAG
Sbjct: 129 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAG 188

Query: 197 VADECEVCAGTGKCRFN 207
           VADECEVCAGTGKCRFN
Sbjct: 189 VADECEVCAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. ExPASy TrEMBL
Match: A0A1S3C8S9 (sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103498093 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 7.5e-99
Identity = 182/197 (92.39%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 17  QVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 76
           Q+    PSPVETRSPYTICA QRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI
Sbjct: 9   QISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 68

Query: 77  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 136
           IVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV
Sbjct: 69  IVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 128

Query: 137 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAG 196
           SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       INHCLSHAAG
Sbjct: 129 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAG 188

Query: 197 VADECEVCAGTGKCRFN 207
           VADECEVCAGTGKCRFN
Sbjct: 189 VADECEVCAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. ExPASy TrEMBL
Match: A0A0A0KP17 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 6.8e-92
Identity = 170/197 (86.29%), Postives = 178/197 (90.36%), Query Frame = 0


Query: 17  QVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 76
           Q+     SPVETR  ++ICA QRS IP+GRLSKFRNFTSR RMSIDNGSSNRIGAGDAVI
Sbjct: 9   QISPKSSSPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNRIGAGDAVI 68

Query: 77  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 136
           IVDHGSRRRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVEQGAKRIIV
Sbjct: 69  IVDHGSRRRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIV 128

Query: 137 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAG 196
           SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       IN+CLSHAAG
Sbjct: 129 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAG 188

Query: 197 VADECEVCAGTGKCRFN 207
           V+DECEVCAGTGKCRFN
Sbjct: 189 VSDECEVCAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. ExPASy TrEMBL
Match: A0A6J1K384 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111489668 PE=4 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 5.6e-86
Identity = 162/193 (83.94%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 23  PSPVETRSPYTICAFQRSP--IPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDH 82
           PS  ETR  YTICA Q+S   I IGR S  RNF+SR RMS+DNG SNRIGAGDAVIIVDH
Sbjct: 15  PSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNRIGAGDAVIIVDH 74

Query: 83  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFF 142
           GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRI+VSPFF
Sbjct: 75  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVVSPFF 134

Query: 143 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAGVADE 202
           LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       I+HCLSHAAGV +E
Sbjct: 135 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAGVVEE 194

Query: 203 CEVCAGTGKCRFN 207
           CEVCAGTGKC+FN
Sbjct: 195 CEVCAGTGKCKFN 207

[Close]

BLAST of IVF0009041 vs. ExPASy TrEMBL
Match: A0A6J1FHT5 (sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111445470 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 6.2e-85
Identity = 159/193 (82.38%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 23  PSPVETRSPYTICAFQRSP--IPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDH 82
           PS  ETR  YTICA Q+S   I +GR S  RNF+SR RMS+DNG ++RIGAGDAVIIVDH
Sbjct: 15  PSAGETRKTYTICASQKSSILIGVGRSSTLRNFSSRFRMSVDNGGADRIGAGDAVIIVDH 74

Query: 83  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFF 142
           GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRI+VSPFF
Sbjct: 75  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVVSPFF 134

Query: 143 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAGVADE 202
           LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       I+HCLSHAAGV +E
Sbjct: 135 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAGVVEE 194

Query: 203 CEVCAGTGKCRFN 207
           CEVCAGTGKC+FN
Sbjct: 195 CEVCAGTGKCKFN 207

[Close]

BLAST of IVF0009041 vs. NCBI nr
Match: KAA0037556.1 (sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa])

HSP 1 Score: 370 bits (950), Expect = 1.54e-128
Identity = 184/197 (93.40%), Postives = 185/197 (93.91%), Query Frame = 0


Query: 17  QVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 76
           Q+    PSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI
Sbjct: 9   QISPKSPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 68

Query: 77  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 136
           IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV
Sbjct: 69  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 128

Query: 137 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAG 196
           SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       INHCLSHAAG
Sbjct: 129 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAG 188

Query: 197 VADECEVCAGTGKCRFN 206
           VADECEVCAGTGKCRFN
Sbjct: 189 VADECEVCAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. NCBI nr
Match: XP_008458790.1 (PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo])

HSP 1 Score: 365 bits (936), Expect = 2.09e-126
Identity = 182/197 (92.39%), Postives = 183/197 (92.89%), Query Frame = 0


Query: 17  QVGHVIPSPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 76
           Q+    PSPVETRSPYTICA QRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI
Sbjct: 9   QISPKSPSPVETRSPYTICASQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVI 68

Query: 77  IVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 136
           IVDHGSRRRESN MLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV
Sbjct: 69  IVDHGSRRRESNRMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIV 128

Query: 137 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAG 196
           SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       INHCLSHAAG
Sbjct: 129 SPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRINHCLSHAAG 188

Query: 197 VADECEVCAGTGKCRFN 206
           VADECEVCAGTGKCRFN
Sbjct: 189 VADECEVCAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. NCBI nr
Match: XP_031742215.1 (sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hypothetical protein Csa_008200 [Cucumis sativus])

HSP 1 Score: 342 bits (876), Expect = 2.91e-117
Identity = 169/190 (88.95%), Postives = 176/190 (92.63%), Query Frame = 0


Query: 24  SPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDHGSR 83
           SPVETR  ++ICA QRS IP+GRLSKFRNFTSR RMSIDNGSSNRIGAGDAVIIVDHGSR
Sbjct: 16  SPVETRRLFSICASQRSSIPVGRLSKFRNFTSRFRMSIDNGSSNRIGAGDAVIIVDHGSR 75

Query: 84  RRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFFLFP 143
           RRESNLMLNEFVAMF+DKTGYPIVEPAHMELAEPSI+DSFT CVEQGAKRIIVSPFFLFP
Sbjct: 76  RRESNLMLNEFVAMFKDKTGYPIVEPAHMELAEPSIKDSFTLCVEQGAKRIIVSPFFLFP 135

Query: 144 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAGVADECEV 203
           GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       IN+CLSHAAGV+DECEV
Sbjct: 136 GRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRINYCLSHAAGVSDECEV 195

Query: 204 CAGTGKCRFN 206
           CAGTGKCRFN
Sbjct: 196 CAGTGKCRFN 205

[Close]

BLAST of IVF0009041 vs. NCBI nr
Match: XP_038890885.1 (sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 331 bits (848), Expect = 2.24e-112
Identity = 168/194 (86.60%), Postives = 173/194 (89.18%), Query Frame = 0


Query: 24  SPVETRSPYTICAFQRSPIPIGRLSKFRNFTSRVRMSIDNGSSNR----IGAGDAVIIVD 83
           S VETR  YTICA Q+SPI I RLS+FRNFTSR RMS+DNGSSNR    IGAGDAVIIVD
Sbjct: 53  SAVETRRKYTICASQKSPILIRRLSRFRNFTSRFRMSVDNGSSNRSGDRIGAGDAVIIVD 112

Query: 84  HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPF 143
           HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF  CVEQGAKRIIVSPF
Sbjct: 113 HGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFALCVEQGAKRIIVSPF 172

Query: 144 FLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAGVAD 203
           FLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       I+HCLSHAAGVA+
Sbjct: 173 FLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEKLVDVVNDRISHCLSHAAGVAE 232

Query: 204 ECEVCAGTGKCRFN 206
           ECEVCAGTGKCRFN
Sbjct: 233 ECEVCAGTGKCRFN 246

[Close]

BLAST of IVF0009041 vs. NCBI nr
Match: XP_022993758.1 (sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 322 bits (826), Expect = 1.29e-109
Identity = 162/193 (83.94%), Postives = 169/193 (87.56%), Query Frame = 0


Query: 23  PSPVETRSPYTICAFQRSPI--PIGRLSKFRNFTSRVRMSIDNGSSNRIGAGDAVIIVDH 82
           PS  ETR  YTICA Q+S I   IGR S  RNF+SR RMS+DNG SNRIGAGDAVIIVDH
Sbjct: 15  PSAGETRKTYTICASQKSSILIGIGRSSTLRNFSSRFRMSVDNGGSNRIGAGDAVIIVDH 74

Query: 83  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSCVEQGAKRIIVSPFF 142
           GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSF SCVEQGAKRI+VSPFF
Sbjct: 75  GSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFASCVEQGAKRIVVSPFF 134

Query: 143 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL-------INHCLSHAAGVADE 202
           LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHE L       I+HCLSHAAGV +E
Sbjct: 135 LFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHEQLVDVVNDRISHCLSHAAGVVEE 194

Query: 203 CEVCAGTGKCRFN 206
           CEVCAGTGKC+FN
Sbjct: 195 CEVCAGTGKCKFN 207

[Close]

BLAST of IVF0009041 vs. TAIR 10
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B )

HSP 1 Score: 222.2 bits (565), Expect = 3.7e-58
Identity = 105/145 (72.41%), Postives = 118/145 (81.38%), Query Frame = 0


Query: 67  NRIGAGDAVIIVDHGSRRRESNLMLNEFVAMFRDKTGYPIVEPAHMELAEPSIRDSFTSC 126
           N IG  D +IIVDHGSRRRESNLML EFV MF++KTGYPIVEPAHMELAEPSI+D+F+ C
Sbjct: 76  NGIGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAHMELAEPSIKDAFSLC 135

Query: 127 VEQGAKRIIVSPFFLFPGRHWQQDIPSLTAEAAKDHPGISYIITAPLGLHENL------- 186
           V+QGAKR++VSPFFLFPGRHW  DIPSLTA+AAK+  GISY+ITAPLG H  L       
Sbjct: 136 VQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITAPLGPHNLLLDVVNDR 195

Query: 187 INHCLSHAAGVADECEVCAGTGKCR 205
           I HCLSH  G ADEC VCAGT KC+
Sbjct: 196 IQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:

BLAST of IVF0009041 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84JH7	5.3e-57	72.41	Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana OX=3702 G...	[more]
P61817	4.7e-13	33.03	Sirohydrochlorin ferrochelatase OS=Bacillus megaterium OX=1404 GN=sirB PE=3 SV=1	[more]
Q58380	1.1e-11	40.00	Sirohydrochlorin cobaltochelatase OS=Methanocaldococcus jannaschii (strain ATCC ...	[more]
P61819	2.6e-11	35.87	Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) ...	[more]
A6UWT5	1.8e-09	35.37	Sirohydrochlorin cobaltochelatase OS=Methanococcus aeolicus (strain ATCC BAA-128...	[more]

BLAST of IVF0009041 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BY55	1.8e-100	93.40	Sirohydrochlorin ferrochelatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3C8S9	7.5e-99	92.39	sirohydrochlorin ferrochelatase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A0A0KP17	6.8e-92	86.29	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G523150 PE=4 SV=1	[more]
A0A6J1K384	5.6e-86	83.94	sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1FHT5	6.2e-85	82.38	sirohydrochlorin ferrochelatase, chloroplastic-like OS=Cucurbita moschata OX=366...	[more]

BLAST of IVF0009041 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0037556.1	1.54e-128	93.40	sirohydrochlorin ferrochelatase [Cucumis melo var. makuwa] >TYK03116.1 sirohydro...	[more]
XP_008458790.1	2.09e-126	92.39	PREDICTED: sirohydrochlorin ferrochelatase, chloroplastic [Cucumis melo]	[more]
XP_031742215.1	2.91e-117	88.95	sirohydrochlorin ferrochelatase, chloroplastic [Cucumis sativus] >KGN51370.1 hyp...	[more]
XP_038890885.1	2.24e-112	86.60	sirohydrochlorin ferrochelatase, chloroplastic isoform X1 [Benincasa hispida]	[more]
XP_022993758.1	1.29e-109	83.94	sirohydrochlorin ferrochelatase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of IVF0009041 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G50170.1	3.7e-58	72.41	sirohydrochlorin ferrochelatase B	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.1400		coord: 71..190 e-value: 2.8E-31 score: 110.3
None	No IPR available	PANTHER	PTHR33542	SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC	coord: 66..203
None	No IPR available	PANTHER	PTHR33542:SF3	SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC	coord: 66..203
None	No IPR available	CDD	cd03416	CbiX_SirB_N	coord: 74..174 e-value: 2.22436E-38 score: 126.147
None	No IPR available	SUPERFAMILY	53800	Chelatase	coord: 73..187
IPR002762	Sirohydrochlorin cobaltochelatase CbiX-like	PFAM	PF01903	CbiX	coord: 80..181 e-value: 7.7E-29 score: 100.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0009041.2	IVF0009041.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0019354	siroheme biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0042802	identical protein binding
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051266	sirohydrochlorin ferrochelatase activity
molecular_function	GO:0016829	lyase activity