IVF0008943 (gene) Melon (IVF77) v1

Overview
NameIVF0008943
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPectinesterase
Locationchr09: 23536519 .. 23541816 (+)
RNA-Seq ExpressionIVF0008943
SyntenyIVF0008943
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAAAGGGCCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATCATCCTTGCAAGTATATATGAACCCCTTTTCTTATTTAAACTCACTTCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAACTACAAGCAGCTCCACACCTCCCACTCTGCCAACACCACTCCGGCCACCTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCGCCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTCCTCCTCATCGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCGTCGAGCCAACCCCATTTCCCTCACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGATGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCCAAGGACCCTTTGGCCCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGGAGCTTCCAATCATGACATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGATGGATTTCAGGATGTGACTGACTTGGGTGTTAAGAATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGAGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAAGGGTTTCCTGAGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGTTCTGGGAAATTCAAGACGGTGGCAGAGGCCATTGAGGCTGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTAAGAACTATACCTTTTTTAAATTCCGCTAGGAAATCAACTCTCAGCTCAGTTCTTGTTTACTTTGGCATAACTATACTTTTTCTCTAAAATATTCTCACATATATAATTTTACTAATTAAATTATCTGTGCAGTCTAAACTATAATCATTTTTTTAAAAAAAAAATAAAGTATATAATTTCATAAAAAATAACAATTTAACACGTGCCCATTTATGAGTGGACATAACTAGTCGCTTTAATTTAGTCAGAGATACCCATAGTGTTATTTTTCTAAAATTATAGTGTTTTAACATTTACAAGAGACTATATATATATATAATGCCCTTTGATTAATACATTTTGAAAGAAATAATGTAATGGGCCGGTGGTTTTCAAAACTTTATTTTGAAGAAGATAGAAATCTAGGGGTATAGTTAGTTGGATTCTTAGAGAGTAGTGGATTGTCATGATTTTCTTTTTAATTAATTAAGTCAATTTTCTTCTTCTTTTTTACCTTTCAAAATTTCAATGATTATTAGTTAATTAACTTCTTTCTCTTGGGAAAGTGGAGAAAGAAAATGGTTTTGATTAAGCCATAATTAATTAACCAACATTAGGTCTCATTATAAAAAGGAATTAGGGAAAACAAGGAAAAAGAAAAGTTTTTCTTTTACAAAACTTTTATCGTGATGTGTTTAATTTATTGACTTCAGTGTCATAATCATGCAAAGCTATTACTTTACCAATTCCTAAAGTCCGTAGAGTTTTTTCATGATAATAATTATACAATCAAAAACAATATTTTATATGTCCCCTCACAATAATCAATCAATTTTAAGTCCTTTTCAATTGAGTTGCTTTCATAATAATAACTCTTAATAACAACCAAAAATTAAAATGAATAAAATTCAACACATGAATAGATATATTCAAATCAATCCCTAGTATAAGAATTTTTTTTTTTTTTTAAAAAAACGATATACATGAGAAATATGAAAATAGTTTAGAAAATGAAAGCAGAGATAGGTTGGGAAATTAAAAATGATGCATCACTAGAATTAATAGTATAGATTTAAAGGCCAACATTTTTAGGAGAATAATTGAAAAATGGTGTGTGTGACAGTGTGTGTTGTGTAGGAGTGGGATGAGAGTAATAGAAGGGAGATAAATGTAATTTTGTAGGTACGAAGAGGAGATTTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGAAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGGTATATACTCTCTCACTCTCTTCTCCCTTTTTCTTTTCCCTTTCAATTTTATTCCAAAACCCCCTTTTTTACTAAAAATTCTCTTCATATAAATATAATTAATTAATTAATTAATTAATTAAAATAGCATTTTGACCCCTTCCCTAGTTTACACACTAGGGTTTGTTTAATATACTTTTAAATGTGTTAAAAAAATCTTAAATTGTTTTTTTTTTAAATTGAAAAAAATTAATAATAAACTAGTAGTATATATTAAATTTACGATTCATTAAATATAGGTTAAAACATTTTTTTTATAAAAAAAAAACAGACACAAAATTTATTTATTAATTTAATTTTTGAGTACAAATGAAGGCTTTTAGTTTTGGCACCGGATATCTAGGGTTTAGAATTATGGTCGTCTGATCTTATTAGTACCAAAAGTGGCCATACTAAACTTTTCTTTCTTCTTTTTCTACTTCAACTTTCTTTTTCCTATTGAATTTTCAAAGTTATATCATCCCCAAATCATGTTAAATCTCTCAATTTTTAATAGAAAAAATAACAATTGAATTCATAACATAATTTTAAAAACTATTTTTTTTATATTTAAAAGAATTTGTAATAAGTAGATTTAAAAATTTTCATAAAAAAAATATATGAATATCAATGGACCCATATGCATGTAGATGTGTTGGAATATTGGATATTTGGTGACAGGATTTTAAGTATTTAATATGAACAGTAATAATAGTGAGTTTAAATCCTAATATTTATTCTAAACATGGTAGATTTGGAAGTTTAGAGACCAAAATAATATTAAGAAAAAACAATGAATTCCTATAGTATTATTATCATCATCATCATTTTTGTTTTAAAAAAAAGGGTTATATTTCAAGATGGTGACAAATTAAGCTACTTTTGTAGCATTTAATGGGCTACAAAAAATAATTGTTTGATTTTATACCCAAATAATCATATGAGAAATAGTAGATTCCAGCTGTGTTGGGTCAAGTAGTCTTCATCATTCATTATTTTTGTAGGAAAAAAAACACCCACCATTCATATTTTATAATTATATGCTATGCTAGAGTGTATACGTTTACTAGTAAAGTCAGGCTTACAAATTGAATATTTGACGTAACATTTTCACATGACATATTCTTAAAATATATATCATAAAGGTTTTCTTACTAGTAAGGATATTATAAAACAAAACTTTGATCATCTTTAATTTTAAAATAACGATAATAATAAAGTACCATAAGATATGTAGTCGTAATATATTAAGCAAGTTGTATTAATTGGTTATTATTTGTAATAAATTTTATATTTCGTATTATAAGAAGATTCCTAGTAGGGTTTTAGAAATCTTTTTGTTATCTCTATAAAAAAAGATGGATGTCTTTAATTATTATATAATATATTGTGCCTATCATTATTAATTTGTAAAGTATGCCACTCTAATAAATTATTATAATAATAATTTTTGGAAGAAGAAGAAGAAGATGAGAATAAAAAAAAAAAAAAAAGGAATGGAATATCTCAATTATTCATGATGATTTAGAAAAGTCATAGTAGTATTATTCCCATGTGTTCTAGCATCATGCTTACTTCCACAAGTTGGTAAAAAAAGTTTTGTTTTCTTTCTTCTTTCTTCTTTCTTCTTTTTTTTTCCTTCAAATGAGAATCTAATATGGTCCAAATGGAGATGCCAAAAGCTTACTCTCCATGGAGTTAAAGGGTAGAAAAATTAGAAAATAATTATTGAATTTTATTTCAAAAGTATTTCTCTATCTCTCATCACATAATCTATGGTCCTCACCAATATAATAAACTTATACAAGTAATTGAAATTTTATTTTTGTTTGTAAATTATAAACTTAGCTTCTATATGGTAAATGATTGTGTTCATGAGCACTTATTTAAACATTAAATATTATCAAGAAAAAGTTTAAAGTTAAAAATTTAAATTTTAAACATTAAATTGTATGTCAGTGTCTAATAGATTAGAAACTTTTTGTACACAGCTGGTTCAGGGACAGGAATAATTCTACGAGACATGTCGTTCGAGAACACGGCAGGACCAAGCAAGCATCAAGCAGTAGCATTACGTCTGAGCGCGGACCACGCGGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCCAATCGTCAATTCTTTCGGGAGTGCGACATCTACGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCTCAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTCGAAGCGTCCAAGGGCTCTTTCCCCACATTTCTGGGTCGACCTTGGAAGTTATACTCGAGAGTGGTGTACATGGTGTCTTCCATGGGGGACCATATTCATCCACAAGGGTGGCTCGAGTGGCAAGGTTCTTTTGCCCTTGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGGGTCATAACTTCGACGGTGGAAGCCTCCAAGTTTACCGTCGGGCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATTATATTATATGTATTTAAATAATTTCTTCAAAAGTTTGTTTTTTTCTTTTTGGAGGTTTTGTTATTTTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATACATCAAACCTTATGACAAATATTTGGATAGGATGGTC

mRNA sequence

AAATAAAGGGCCCAACCCCACTTTATTATTACAAACATATAGTGGAATAATAATCATCCTTGCAAGTATATATGAACCCCTTTTCTTATTTAAACTCACTTCCACCATTATTCCCTCCCCATGCACACAAATAACCGACCATGAATAACAATAACAACTACAAGCAGCTCCACACCTCCCACTCTGCCAACACCACTCCGGCCACCTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCGCCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTCCTCCTCATCGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCGTCGAGCCAACCCCATTTCCCTCACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGATGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCCAAGGACCCTTTGGCCCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGGAGCTTCCAATCATGACATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGATGGATTTCAGGATGTGACTGACTTGGGTGTTAAGAATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGAGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAAGGGTTTCCTGAGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGTTCTGGGAAATTCAAGACGGTGGCAGAGGCCATTGAGGCTGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGATTTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGAAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCTGGTTCAGGGACAGGAATAATTCTACGAGACATGTCGTTCGAGAACACGGCAGGACCAAGCAAGCATCAAGCAGTAGCATTACGTCTGAGCGCGGACCACGCGGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCCAATCGTCAATTCTTTCGGGAGTGCGACATCTACGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCTCAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTCGAAGCGTCCAAGGGCTCTTTCCCCACATTTCTGGGTCGACCTTGGAAGTTATACTCGAGAGTGGTGTACATGGTGTCTTCCATGGGGGACCATATTCATCCACAAGGGTGGCTCGAGTGGCAAGGTTCTTTTGCCCTTGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGGGTCATAACTTCGACGGTGGAAGCCTCCAAGTTTACCGTCGGGCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAAAAATGCATATATTATATTATATGTATTTAAATAATTTCTTCAAAAGTTTGTTTTTTTCTTTTTGGAGGTTTTGTTATTTTGTATAGTCTCATTCCTTTGATTATGCTCTTCCAAAATAATATGGGCCATACATCAAACCTTATGACAAATATTTGGATAGGATGGTC

Coding sequence (CDS)

ATGAATAACAATAACAACTACAAGCAGCTCCACACCTCCCACTCTGCCAACACCACTCCGGCCACCTCCTTCCGCCTCTCCGGCAATGATCCCCCTCCGCCCTCCTCCTCCCTCCGTCGTCGCTCTCGTATCCTAATCATCTCTTTCATGTCCGTCCTCCTCATCGCTGCTTCAGTCGTTTCCGCAGCTATCCTTGTTCGATCACGTATCGCGTCGAGCCAACCCCATTTCCCTCACAACAAGCCCTCCCAAGCCATCTCCCGCGCGTGTAGCCACACGCTCTACCCATCTCTTTGCCTCTCCTCTCTTCTATCCTTCCCCGGCGCACAATCGGCCGATGTTCATGACCTTGTTCATATCTCTCTCAACTTAACTCTTCAGCATCTCACCAAAGCTCTTTATTCCACTTCTCAAATCCCTATCTTACAAATCTCCAAGGACCCTTTGGCCCATTCCGCTTATGAAGATTGTATGGAGCTTTTAAACGACGCCATTGATGCTTTTTCTCTCTCTCTTTTCTCTACGGGAGCTTCCAATCATGACATCATGACGTGGCTTAGTGCTGCATTGACTTACCATGACACGTGTACGGATGGATTTCAGGATGTGACTGACTTGGGTGTTAAGAATGAGGTGGAGGCCAAGTTGAGTGATTTGTCGGAGATGATTAGTAATTCTTTGGCTATATTTTCTGGGTTTGGAGGTGGGGATTTGCCGGTGGAGAATAGAAAGCGGCGGCGGTTGATGGAGAGTGGTACGCCGTCGTCGTGGGCGGCGGAGAATGGTGGTGATCATGAAGGGTTTCCTGAGTGGTTGAGTGGGAAGGATCGGCGGCTTTTGGCTGCTCCTATATCGGCGATTCAGGCGGATATTGTGGTAGCTAAGGATGGTTCTGGGAAATTCAAGACGGTGGCAGAGGCCATTGAGGCTGCTCCGTCGTCGAGTGGCCGCCGGATCATCATCTACATTAAGGCTGGAAAGTACGAAGAGGAGATTTTGAAGGTGGGAAGGAAGAAAACAAACTTGATGTTCGTGGGAGATGGGAAGGGCATAACCGTCATTTCAGGTGGAAAAAGTGTTTATGACAAAGTTACCACTTTTCGCACCGCCACCTTTGCTGGTTCAGGGACAGGAATAATTCTACGAGACATGTCGTTCGAGAACACGGCAGGACCAAGCAAGCATCAAGCAGTAGCATTACGTCTGAGCGCGGACCACGCGGTGGTATACCGTTGCAACATCATTGGTTATCAAGACACTCTCTACGTGCACTCCAATCGTCAATTCTTTCGGGAGTGCGACATCTACGGAACAATCGACTTCATCTTCGGCAACGCTGTGGTGGTTTTCCAAAGCTGCAACATCTACGCCCGAAAGCCAATGGCAGGACAAAAAAACACGATAACAGCTCAAGACCGAAAGGACCCGAACCAAAACACAGGAATCTCAATCCACGCTTGCAAGATCGTAGCCACCGGCGACCTCGAAGCGTCCAAGGGCTCTTTCCCCACATTTCTGGGTCGACCTTGGAAGTTATACTCGAGAGTGGTGTACATGGTGTCTTCCATGGGGGACCATATTCATCCACAAGGGTGGCTCGAGTGGCAAGGTTCTTTTGCCCTTGACACTCTTTATTACGGCGAGTATATGAATTCTGGGCCGGGGGCCGCCGTGGGGCAGCGGGTCAAGTGGCCGGGTTACCGGGTCATAACTTCGACGGTGGAAGCCTCCAAGTTTACCGTCGGGCAATTTATTTACGGTTCTTCTTGGTTGCCTTCTACCGGAGTCTCCTTTTCTGCTGGATTATCTACTAACTAA

Protein sequence

MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLSTN
Homology
BLAST of IVF0008943 vs. ExPASy Swiss-Prot
Match: Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 8.7e-201
Identity = 366/585 (62.56%), Postives = 441/585 (75.38%), Query Frame = 0

Query: 30  DPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPH--NKPSQAIS 89
           DP      L+++++  +I F   +L+   VV   I    R   S    P    KP+QAIS
Sbjct: 18  DPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAIS 77

Query: 90  RACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKD 149
           R CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S I   Q+   
Sbjct: 78  RTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--P 137

Query: 150 PLAHSAYEDCMELLNDAIDAFSLSLFS----TGASNH-DIMTWLSAALTYHDTCTDGFQD 209
           P   SAY+ C+ELL+D++DA + +L S    +G  +H D+MTWLS+A+T HDTCTDGF +
Sbjct: 138 PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDE 197

Query: 210 VTDLG--VKNEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRLMESGTPSSWA 269
           +   G  VK++V   + DLSEM+SN LAIF+G       +PV N   R+L+  GT  +  
Sbjct: 198 IEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKLL--GTEET-- 257

Query: 270 AENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRR 329
                  E  P WL  +DR LL  P SAIQADI V+KDGSG FKT+AEAI+ AP  S RR
Sbjct: 258 -------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRR 317

Query: 330 IIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTG 389
            +IY+KAG+YEEE LKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G G
Sbjct: 318 FVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAG 377

Query: 390 IILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 449
            I+RDM+FEN AGP+KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYGT
Sbjct: 378 FIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGT 437

Query: 450 IDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEAS 509
           +DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK++AT DLEAS
Sbjct: 438 VDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEAS 497

Query: 510 KGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAV 569
           KGS+PT+LGRPWKLYSRVVYM+S MGDHI P+GWLEW G FALD+LYYGEYMN G G+ +
Sbjct: 498 KGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGI 557

Query: 570 GQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 604
           GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 558 GQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of IVF0008943 vs. ExPASy Swiss-Prot
Match: Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)

HSP 1 Score: 682.2 bits (1759), Expect = 5.4e-195
Identity = 345/600 (57.50%), Postives = 443/600 (73.83%), Query Frame = 0

Query: 13  SHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTGASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S+ A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT+GF  V D GVK+ + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 312
           +   + E               E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT
Sbjct: 254 RLLGVEER-------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKT 313

Query: 313 VAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDK 372
           ++EAI+ AP +S RRIIIY+KAG+YEE  LKVGRKK NLMFVGDGKG TVISGGKS++D 
Sbjct: 314 ISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDN 373

Query: 373 VTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYV 432
           +TTF TA+FA +G G I RD++FEN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYV
Sbjct: 374 ITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYV 433

Query: 433 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 492
           HSNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGIS
Sbjct: 434 HSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGIS 493

Query: 493 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDT 552
           IHA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H +GWLEW  +FALDT
Sbjct: 494 IHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDT 553

Query: 553 LYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 604
           LYYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 LYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of IVF0008943 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 466.5 bits (1199), Expect = 4.7e-130
Identity = 256/587 (43.61%), Postives = 363/587 (61.84%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQAISR-ACSHTLY 96
           + ++  +++++S    LL  A+V      + +  + +      +  S A+ R +CS T Y
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72

Query: 97  PSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
           P LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A  
Sbjct: 73  PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132

Query: 157 DCMELLNDAIDAF-----SLSLFSTGAS--NH--DIMTWLSAALTYHDTCTDGF-QDVTD 216
           DC+E +++ +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF  D  D
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192

Query: 217 LGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK------RRRLMESGTPSSWAA 276
             V+  +      +  M SN+LA+       D+    +K       R+L E    ++ A 
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252

Query: 277 ENGG----DHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
           +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312

Query: 337 GRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
            +R +I+IKAG Y E + +V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  
Sbjct: 313 NKRYVIHIKAGVYRENV-EVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372

Query: 397 GTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDI 456
           G   + RD++F+NTAGPSKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432

Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
            GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492

Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPG 576
           ++ KGSFPT+LGRPWK YS+ V M S++ D I P+GW EW G+FAL+TL Y EY N+G G
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 552

Query: 577 AAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           A    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of IVF0008943 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 460.7 bits (1184), Expect = 2.6e-128
Identity = 254/579 (43.87%), Postives = 344/579 (59.41%), Query Frame = 0

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQAISRACSHTLY 95
           S  +   ++++ S    LL+ AS+V  A    ++  + +     +     +   CS TLY
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71

Query: 96  PSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 155
           P LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A  
Sbjct: 72  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131

Query: 156 DCMELLNDAIDAFSLSL---------FSTGASNHDIMTWLSAALTYHDTCTDGFQ-DVTD 215
           DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF  D  D
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191

Query: 216 LGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESGTPSSWAAENGG- 275
             V+  +      +  M SN+LA+       D+   E R +     +          G  
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251

Query: 276 DHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYI 335
           D +G+P+WLS  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R +I+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311

Query: 336 KAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRD 395
           KAG Y E + +V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + RD
Sbjct: 312 KAGVYRENV-EVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371

Query: 396 MSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIF 455
           ++F+NTAGPSKHQAVALR+ +D +  Y+C++  YQDTLYVHSNRQFF +C I GT+DFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431

Query: 456 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFP 515
           GNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491

Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 575
           T+LGRPWK YSR V M S + D I P+GW EW GSFALDTL Y EY+N G GA    RVK
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVK 551

Query: 576 WPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           W GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 WKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of IVF0008943 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 4.9e-127
Identity = 261/591 (44.16%), Postives = 358/591 (60.58%), Query Frame = 0

Query: 25  RLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQ 84
           +LS +      S++ ++ + L ++  + LL+ A+V+     V SR  S       N+P  
Sbjct: 10  KLSESSSNQNISNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGD---NGNEPHH 69

Query: 85  AISR-ACSHTLYPSLCLSSLLSFPGAQS--ADVHDLVHISLNLTLQHLTKALYSTSQIPI 144
           AI + +CS T YP LC S++ + P A        D++ +SLN+T   +    +   ++ +
Sbjct: 70  AILKSSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-L 129

Query: 145 LQISKDPLAHSAYEDCMELLNDAIDAFSLSL-------FSTGASNH--DIMTWLSAALTY 204
            + +       A  DC+E +++ +D    ++            S H  D+ T +SAA+T 
Sbjct: 130 KRTNLTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTN 189

Query: 205 HDTCTDGF-QDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMES 264
             TC DGF  D  +  V++ +      + +M SN+LA+       D+ +      R +  
Sbjct: 190 QGTCLDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTE 249

Query: 265 GTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAA 324
            T +          +G+P WLS  DRRLL +  S++  + VVA DGSG FKTVA A+ AA
Sbjct: 250 ETSTV---------DGWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAA 309

Query: 325 PSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTAT 384
           P    +R II IKAG Y E + +V +K  N+MF+GDG+  T+I+G ++V D  TTF++AT
Sbjct: 310 PQGGTKRYIIRIKAGVYRENV-EVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSAT 369

Query: 385 FAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFR 444
            A  G G + RD++F+NTAGPSKHQAVALR+ AD +  Y C+++ YQDTLYVHSNRQFF 
Sbjct: 370 AAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFV 429

Query: 445 ECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVA 504
            C I GT+DFIFGNA  V Q+C+I+ARKP +GQKN +TAQ R DPNQNTGI I   +I A
Sbjct: 430 NCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGA 489

Query: 505 TGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMN 564
           T DL+  +GSFPT+LGRPWK YSR V M SS+ D IHP GW EW G+FAL+TL+YGE+ N
Sbjct: 490 TSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQN 549

Query: 565 SGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           SG GA    RVKW G+RVITS  EA  FT G FI GSSWL STG  FS GL
Sbjct: 550 SGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of IVF0008943 vs. ExPASy TrEMBL
Match: A0A1S3AUZ6 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1)

HSP 1 Score: 1194.1 bits (3088), Expect = 0.0e+00
Identity = 603/605 (99.67%), Postives = 604/605 (99.83%), Query Frame = 0

Query: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
           SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
           YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 606
           GLSTN
Sbjct: 601 GLSTN 605

BLAST of IVF0008943 vs. ExPASy TrEMBL
Match: A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 581/605 (96.03%), Postives = 590/605 (97.52%), Query Frame = 0

Query: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 606
           GLSTN
Sbjct: 601 GLSTN 604

BLAST of IVF0008943 vs. ExPASy TrEMBL
Match: A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)

HSP 1 Score: 1016.9 bits (2628), Expect = 3.4e-293
Identity = 517/605 (85.45%), Postives = 553/605 (91.40%), Query Frame = 0

Query: 4   NNNYKQLHTSHSANTTPATSFRLSG--NDPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNY  LH+SHS + TPA+SFR+S   +DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGD-TPASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSRI-ASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV SRI AS++ HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCTDGF DV D  +K+++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 303
           ENR+RRRLMES     W AE       FP W+SGKDRRLLAAPIS+IQADIVVAKDGSGK
Sbjct: 242 ENRRRRRLMES--DEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGK 301

Query: 304 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 363
           FKTVAEAI AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 302 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 361

Query: 364 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 423
           YDKVTTFRTATFAGSGTG+ILRDM+FENTAGP+KHQAVALRLSADHAVV+R N+IGYQDT
Sbjct: 362 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 421

Query: 424 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 483
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQNT
Sbjct: 422 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 481

Query: 484 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 543
           GISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFA
Sbjct: 482 GISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 541

Query: 544 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 603
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 542 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSA 601

Query: 604 GLSTN 606
           GLSTN
Sbjct: 602 GLSTN 602

BLAST of IVF0008943 vs. ExPASy TrEMBL
Match: A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)

HSP 1 Score: 1005.4 bits (2598), Expect = 1.0e-289
Identity = 513/608 (84.38%), Postives = 550/608 (90.46%), Query Frame = 0

Query: 4   NNNYKQLHTSHSANTTPATSFRLSGN-----DPPPPSSSLRRRSRILIISFMSVLLIAAS 63
           NNNY +LH+S S + TP TSFR+S +      PPPPSSS R   RIL+ISF S+LLI AS
Sbjct: 2   NNNYNKLHSSRSGD-TPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITAS 61

Query: 64  VVSAAILVRSRI-ASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDL 123
            VSA +LV SRI  S++ HFPHNKP+QAISRACS TLYPSLC+SSLLS PG+ SADVHDL
Sbjct: 62  TVSAVVLVHSRITTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 121

Query: 124 VHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGA 183
           VHISLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A
Sbjct: 122 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 181

Query: 184 SNHDIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGD 243
           +NHD+MTWLSAALTYHDTCTDGF DV D  VK+++ AKLSDLSEMISNSLAIFSGFGGGD
Sbjct: 182 TNHDVMTWLSAALTYHDTCTDGFSDVAD-PVKDQITAKLSDLSEMISNSLAIFSGFGGGD 241

Query: 244 LPVENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDG 303
           LPVENR+RRRLMES     W AE       FP W+SGKDRRLLAAPIS+IQADIVVAKDG
Sbjct: 242 LPVENRRRRRLMES--DEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDG 301

Query: 304 SGKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGG 363
           SGKFKTVAEAI AAPSSS RRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGG
Sbjct: 302 SGKFKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGG 361

Query: 364 KSVYDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGY 423
           KSVYDKVTTFRTATFAGSGTG+ILRDM+FENTAGP+KHQAVALRLSADH VV+R N+IGY
Sbjct: 362 KSVYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGY 421

Query: 424 QDTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPN 483
           QDTLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPN
Sbjct: 422 QDTLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPN 481

Query: 484 QNTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQG 543
           QNTGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSS+GDHIHP+GWLEWQG
Sbjct: 482 QNTGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQG 541

Query: 544 SFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVS 603
           SFALDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVS
Sbjct: 542 SFALDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVS 601

Query: 604 FSAGLSTN 606
           FSAGLSTN
Sbjct: 602 FSAGLSTN 602

BLAST of IVF0008943 vs. ExPASy TrEMBL
Match: A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 1.4e-281
Identity = 507/603 (84.08%), Postives = 547/603 (90.71%), Query Frame = 0

Query: 5   NNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAI 64
           NNY +LH+S S + TPAT F++S    PP SS+ RRR+RIL +S +S+LLI AS VSAA+
Sbjct: 2   NNYNKLHSSASGD-TPAT-FQVSHTPNPPSSSASRRRTRILFVSLISILLITASAVSAAV 61

Query: 65  LVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNL 124
           L+ S+      HFPHNKP+QAIS ACSHTLYPSLC SSLLSFPG+ SADVH LVHISLNL
Sbjct: 62  LLHSQ----SSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHRLVHISLNL 121

Query: 125 TLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNHDIMT 184
           TLQHLTKALY++SQIP+ QIS +PLAHSAY+DC+ELLNDA+DAFSLSLFST ASNHDIMT
Sbjct: 122 TLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTSASNHDIMT 181

Query: 185 WLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK 244
           WLSAALTYHDTC+DGF DV D  VK+++ A+LSDLSEMISNSLAIFSG+G G LPV+N  
Sbjct: 182 WLSAALTYHDTCSDGFADVDD-PVKDQMAARLSDLSEMISNSLAIFSGYGEG-LPVQN-- 241

Query: 245 RRRLMESGTPSSWAAENG-GDHEG-FPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFK 304
           RRRLMES     WAAENG    EG FP W+SGKDRRLLAAPISAIQADIVVAKDGSGKFK
Sbjct: 242 RRRLMES--DREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGSGKFK 301

Query: 305 TVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYD 364
           TVAEAIEAAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV+D
Sbjct: 302 TVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSVFD 361

Query: 365 KVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLY 424
           KVTTFRTATFAGSGTGIILRDM+FENTAGPSKHQAVALR+SADHAVVYRCNIIGYQDTLY
Sbjct: 362 KVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQDTLY 421

Query: 425 VHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGI 484
           VHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM  QKNTITAQ+RKDPNQNTGI
Sbjct: 422 VHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQNTGI 481

Query: 485 SIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALD 544
           SIHACKI+AT DLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFALD
Sbjct: 482 SIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFALD 541

Query: 545 TLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 604
           TLYYGEYMNSGPGAA GQRV WPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFSAGL
Sbjct: 542 TLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSAGL 592

Query: 605 STN 606
           S N
Sbjct: 602 SAN 592

BLAST of IVF0008943 vs. NCBI nr
Match: XP_008437596.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 61 [Cucumis melo])

HSP 1 Score: 1193 bits (3086), Expect = 0.0
Identity = 603/605 (99.67%), Postives = 604/605 (99.83%), Query Frame = 0

Query: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
           SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180

Query: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
           YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQK TITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKXTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 605
           GLSTN
Sbjct: 601 GLSTN 605

BLAST of IVF0008943 vs. NCBI nr
Match: XP_011654619.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.1 hypothetical protein Csa_000423 [Cucumis sativus])

HSP 1 Score: 1148 bits (2969), Expect = 0.0
Identity = 581/605 (96.03%), Postives = 590/605 (97.52%), Query Frame = 0

Query: 1   MNNNNNYKQLHTSHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60
           MNNNN+Y +LHTSHSANTTPA SFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV
Sbjct: 1   MNNNNSYNKLHTSHSANTTPAASFRLSGNDPPPPSSSLRRRSRILIISFMSVLLIAASVV 60

Query: 61  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120
           SAAILVRS + SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI
Sbjct: 61  SAAILVRSHMTSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 120

Query: 121 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 180
           SLNLTLQHLTKALYSTSQIP+LQISKDPLAHSAYEDCMELLNDAIDAFSLSLFS  ASNH
Sbjct: 121 SLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDASNH 180

Query: 181 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240
           DIMTWLSAALTYHDTCT GFQDV DLGVK+EVEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 181 DIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 240

Query: 241 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 300
           ENRKRRRLMES T +SWAAENGGDHEGFP WLSGKDRRLLAAP+S IQADIVVAKDGSGK
Sbjct: 241 ENRKRRRLMESST-TSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGSGK 300

Query: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 360
           FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 301 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 360

Query: 361 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 420
           YDKVTTFRTATFAGSGT IILRDM+FENTAGPSKHQAVALRLSADHAVVY CNIIGYQDT
Sbjct: 361 YDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 420

Query: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480
           LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT
Sbjct: 421 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 480

Query: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 540
           GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSFA
Sbjct: 481 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 540

Query: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600
           LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 541 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 600

Query: 601 GLSTN 605
           GLSTN
Sbjct: 601 GLSTN 604

BLAST of IVF0008943 vs. NCBI nr
Match: XP_038874841.1 (probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida])

HSP 1 Score: 1078 bits (2787), Expect = 0.0
Identity = 551/605 (91.07%), Postives = 573/605 (94.71%), Query Frame = 0

Query: 4   NNNYKQLHTSHSANTTPATSFRLSGND---PPPPSSSLRRRSRILIISFMSVLLIAASVV 63
           NNNY +LH+S SA +TPA+SF LSGND    PPPSSS RRRSRILIISF+SVLLIAAS+V
Sbjct: 3   NNNYNKLHSSLSATSTPASSFHLSGNDLPPTPPPSSSHRRRSRILIISFLSVLLIAASIV 62

Query: 64  SAAILVRSRIASSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           SAA+LVRSRIAS++PHFPHNKPSQAISRACSHTLYPSLCLSSLLS P A SADVHDLVHI
Sbjct: 63  SAAVLVRSRIASNEPHFPHNKPSQAISRACSHTLYPSLCLSSLLSIPDALSADVHDLVHI 122

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLAHSAYEDCMELLNDA+DAFS+SLFST ASNH
Sbjct: 123 SLNLTLQHLTKALYSTSQIPISQISNDPLAHSAYEDCMELLNDAVDAFSVSLFSTEASNH 182

Query: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCTDGF DV DL VKN+VEAKLSDLSEMISNSLAIFSGFGGGDLPV
Sbjct: 183 DVMTWLSAALTYHDTCTDGFADVADLAVKNQVEAKLSDLSEMISNSLAIFSGFGGGDLPV 242

Query: 244 ENRKRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGK 303
           +NR RRRLMESGT  SWAAENGGD  GFP+WLS KDRRLLAAPISAIQADIVVAKDGSGK
Sbjct: 243 QNR-RRRLMESGT-RSWAAENGGDG-GFPQWLSVKDRRLLAAPISAIQADIVVAKDGSGK 302

Query: 304 FKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSV 363
           FKTVAEAI+AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKSV
Sbjct: 303 FKTVAEAIDAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362

Query: 364 YDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 423
           +DKVTTFRTATFAGSGTGIILRDM FENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT
Sbjct: 363 FDKVTTFRTATFAGSGTGIILRDMIFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDT 422

Query: 424 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNT 483
           LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM GQKNTITAQDRKDPNQNT
Sbjct: 423 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPGQKNTITAQDRKDPNQNT 482

Query: 484 GISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFA 543
           GISIHACKI+AT DL+ASKGSFPTFLGRPWKLYSRVVYMVS MGDHIHP+GWLEWQGSFA
Sbjct: 483 GISIHACKIIATSDLQASKGSFPTFLGRPWKLYSRVVYMVSFMGDHIHPRGWLEWQGSFA 542

Query: 544 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 603
           LDTLYYGEY NSGPGAAVGQRVKW GYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 543 LDTLYYGEYSNSGPGAAVGQRVKWSGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSA 602

Query: 604 GLSTN 605
           GLS N
Sbjct: 603 GLSAN 604

BLAST of IVF0008943 vs. NCBI nr
Match: XP_022922302.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])

HSP 1 Score: 1016 bits (2626), Expect = 0.0
Identity = 520/606 (85.81%), Postives = 557/606 (91.91%), Query Frame = 0

Query: 4   NNNYKQLHTSHSANTTPATSFRLSGN--DPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNY  LH+SHS +T PA+SFR+S +  DP PP S    R RIL+ISF S+LLI AS VS
Sbjct: 2   NNNYNMLHSSHSGDT-PASSFRVSNDHSDPLPPPSPSSSRRRILLISFFSILLITASAVS 61

Query: 64  AAILVRSRIASS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV SRIA+S + HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCTDGF DV D  +K+++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSSWAAE-NGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSG 303
           ENR+RRRLMES     W AE N GD + FP W+SGKDRRLLAAPIS+IQADIVVAKDGSG
Sbjct: 242 ENRRRRRLMES--DEQWLAEENNGDGD-FPRWVSGKDRRLLAAPISSIQADIVVAKDGSG 301

Query: 304 KFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKS 363
           KFKTVAEAI AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 302 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 361

Query: 364 VYDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQD 423
           VYDKVTTFRTATFAGSGTG+ILRDM+FENTAGP+KHQAVALRLSADHAVV+R N+IGYQD
Sbjct: 362 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 421

Query: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQN 483
           TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPM+GQKNTITAQDRKDPNQN
Sbjct: 422 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 481

Query: 484 TGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSF 543
           TGISIHACKI+ATGDL+ASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSF
Sbjct: 482 TGISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541

Query: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFS 601

Query: 604 AGLSTN 605
           AGLSTN
Sbjct: 602 AGLSTN 602

BLAST of IVF0008943 vs. NCBI nr
Match: KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1014 bits (2622), Expect = 0.0
Identity = 519/606 (85.64%), Postives = 558/606 (92.08%), Query Frame = 0

Query: 4   NNNYKQLHTSHSANTTPATSFRLSGN--DPPPPSSSLRRRSRILIISFMSVLLIAASVVS 63
           NNNY +LH+S S +T PA+SFR+S +  DP PP S    R RIL+ISF+S+ LI AS VS
Sbjct: 2   NNNYNKLHSSRSGDT-PASSFRVSNDHSDPLPPPSPSSSRRRILLISFLSIFLITASAVS 61

Query: 64  AAILVRSRIASS-QPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDLVHI 123
           A +LV SRIA+S + HFPHNKP+QAISRACS TLYPSLCLSSLLS PG+ SADVHDLVHI
Sbjct: 62  AVVLVHSRIAASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDLVHI 121

Query: 124 SLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSTGASNH 183
           SLNLTLQHLTKALYSTSQIPI QIS DPLA SAYEDCMELLNDA++AFSLSLFST A+NH
Sbjct: 122 SLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTATNH 181

Query: 184 DIMTWLSAALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPV 243
           D+MTWLSAALTYHDTCTDGF DV D  +K+++ AKLSDLSEMISNSLAIFSGFGG DLPV
Sbjct: 182 DVMTWLSAALTYHDTCTDGFSDVAD-PIKDQITAKLSDLSEMISNSLAIFSGFGGDDLPV 241

Query: 244 ENRKRRRLMESGTPSSWAAE-NGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSG 303
           ENR+RRRLMES     W AE N GD + FP+W+SGKDRRLLAAPIS+IQADIVVAKDGSG
Sbjct: 242 ENRRRRRLMES--DEHWLAEENNGDGD-FPQWVSGKDRRLLAAPISSIQADIVVAKDGSG 301

Query: 304 KFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKS 363
           KFKTVAEAI AAPSSSGRRI+IYIKAGKYEEE LKVGRKKTNLMFVGDGKGITVISGGKS
Sbjct: 302 KFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS 361

Query: 364 VYDKVTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQD 423
           VYDKVTTFRTATFAGSGTG+ILRDM+FENTAGP+KHQAVALRLSADHAVV+R N+IGYQD
Sbjct: 362 VYDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQD 421

Query: 424 TLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQN 483
           TLYVHSNRQFFRECDIYGTIDFIFGNA VVFQSCNIYARKPM+GQKNTITAQDRKDPNQN
Sbjct: 422 TLYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQN 481

Query: 484 TGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSF 543
           TGISIHACKI+ATGDLEASKGSFPT+LGRPWKLYSRVVYMVSSMGDHIHP+GWLEWQGSF
Sbjct: 482 TGISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSF 541

Query: 544 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFS 603
           ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSFS
Sbjct: 542 ALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFS 601

Query: 604 AGLSTN 605
           AGLSTN
Sbjct: 602 AGLSTN 602

BLAST of IVF0008943 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 701.4 bits (1809), Expect = 6.2e-202
Identity = 366/585 (62.56%), Postives = 441/585 (75.38%), Query Frame = 0

Query: 30  DPPPPSSSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPH--NKPSQAIS 89
           DP      L+++++  +I F   +L+   VV   I    R   S    P    KP+QAIS
Sbjct: 18  DPATSLPELQKKTKTKLILFTLAVLV-VGVVCFGIFAGIRAVDSGKTEPKLTRKPTQAIS 77

Query: 90  RACSHTLYPSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKD 149
           R CS +LYP+LC+ +LL FPG+ +AD ++L+HIS N TLQ  +KALY++S I   Q+   
Sbjct: 78  RTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSSTITYTQM--P 137

Query: 150 PLAHSAYEDCMELLNDAIDAFSLSLFS----TGASNH-DIMTWLSAALTYHDTCTDGFQD 209
           P   SAY+ C+ELL+D++DA + +L S    +G  +H D+MTWLS+A+T HDTCTDGF +
Sbjct: 138 PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTNHDTCTDGFDE 197

Query: 210 VTDLG--VKNEVEAKLSDLSEMISNSLAIFSG--FGGGDLPVENRKRRRLMESGTPSSWA 269
           +   G  VK++V   + DLSEM+SN LAIF+G       +PV N   R+L+  GT  +  
Sbjct: 198 IEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN--NRKLL--GTEET-- 257

Query: 270 AENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRR 329
                  E  P WL  +DR LL  P SAIQADI V+KDGSG FKT+AEAI+ AP  S RR
Sbjct: 258 -------EELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPEHSSRR 317

Query: 330 IIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTG 389
            +IY+KAG+YEEE LKVGRKKTNLMF+GDGKG TVI+GGKS+ D +TTF TATFA +G G
Sbjct: 318 FVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFAATGAG 377

Query: 390 IILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGT 449
            I+RDM+FEN AGP+KHQAVALR+  DHAVVYRCNIIGYQD LYVHSNRQFFREC+IYGT
Sbjct: 378 FIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFRECEIYGT 437

Query: 450 IDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEAS 509
           +DFIFGNA V+ QSCNIYARKPMA QK TITAQ+RKDPNQNTGISIHACK++AT DLEAS
Sbjct: 438 VDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATPDLEAS 497

Query: 510 KGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAV 569
           KGS+PT+LGRPWKLYSRVVYM+S MGDHI P+GWLEW G FALD+LYYGEYMN G G+ +
Sbjct: 498 KGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKGLGSGI 557

Query: 570 GQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 604
           GQRVKWPGY VITSTVEASKFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 558 GQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586

BLAST of IVF0008943 vs. TAIR 10
Match: AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 682.2 bits (1759), Expect = 3.9e-196
Identity = 345/600 (57.50%), Postives = 443/600 (73.83%), Query Frame = 0

Query: 13  SHSANTTPATSFRLSGNDPPPPSSSLRRRSRILIISF-MSVLLIAASVVSAAILVRSRIA 72
           S + +TT A           P +++ R + ++++ S  +++ LI A+ + A +  R ++ 
Sbjct: 14  SVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRSRLKLN 73

Query: 73  SSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSA-DVHDLVHISLNLTLQHLT 132
            S P     KPSQAIS+AC  T +P LC+ SL+ FPG+ +A    DL+H+++N+TL H +
Sbjct: 74  QSVPGLA-RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTVNMTLHHFS 133

Query: 133 KALYSTSQIPILQISKDPLAHSAYEDCMELLNDAIDAFS---LSLFSTGASNHDIMTWLS 192
            ALYS++ +    +   P A SAY+ C+ELL+D++DA S    S+ S+ A   D+ TWLS
Sbjct: 134 HALYSSASLSF--VDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKPQDVTTWLS 193

Query: 193 AALTYHDTCTDGFQDVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGD----LPVENR 252
           AALT HDTCT+GF  V D GVK+ + A L +LSE++SN LAIFS    GD    +P++NR
Sbjct: 194 AALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDFAGVPIQNR 253

Query: 253 KRRRLMESGTPSSWAAENGGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKT 312
           +   + E               E FP W+  K+R +L  P+S IQADI+V+KDG+G  KT
Sbjct: 254 RLLGVEER-------------EEKFPRWMRPKEREILEMPVSQIQADIIVSKDGNGTCKT 313

Query: 313 VAEAIEAAPSSSGRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDK 372
           ++EAI+ AP +S RRIIIY+KAG+YEE  LKVGRKK NLMFVGDGKG TVISGGKS++D 
Sbjct: 314 ISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGGKSIFDN 373

Query: 373 VTTFRTATFAGSGTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYV 432
           +TTF TA+FA +G G I RD++FEN AGP+KHQAVALR+ ADHAV+YRCNIIGYQDTLYV
Sbjct: 374 ITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGYQDTLYV 433

Query: 433 HSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGIS 492
           HSNRQFFRECDIYGT+DFIFGNA VV Q+C+IYARKPM  QKNTITAQ+RKDPNQNTGIS
Sbjct: 434 HSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPNQNTGIS 493

Query: 493 IHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDT 552
           IHA +++A  DL+A+ GS  T+LGRPWKL+SR VYM+S +G H+H +GWLEW  +FALDT
Sbjct: 494 IHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNTTFALDT 553

Query: 553 LYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGLS 604
           LYYGEY+NSGPG+ +GQRV WPGYRVI ST EA++FTV +FIYGSSWLPSTGVSF AGLS
Sbjct: 554 LYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVSFLAGLS 597

BLAST of IVF0008943 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 466.5 bits (1199), Expect = 3.3e-131
Identity = 256/587 (43.61%), Postives = 363/587 (61.84%), Query Frame = 0

Query: 37  SLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQAISR-ACSHTLY 96
           + ++  +++++S    LL  A+V      + +  + +      +  S A+ R +CS T Y
Sbjct: 13  NFKKNKKLVLLSAAVALLFVAAVAG----ISAGASKANEKRTLSPSSHAVLRSSCSSTRY 72

Query: 97  PSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 156
           P LC+S++++  G +     D++  S+NLT+  +    ++  ++   +    P   +A  
Sbjct: 73  PELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALH 132

Query: 157 DCMELLNDAIDAF-----SLSLFSTGAS--NH--DIMTWLSAALTYHDTCTDGF-QDVTD 216
           DC+E +++ +D        L L+ T  +   H  D+ T +S+A+T  +TC DGF  D  D
Sbjct: 133 DCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDAD 192

Query: 217 LGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRK------RRRLMESGTPSSWAA 276
             V+  +      +  M SN+LA+       D+    +K       R+L E    ++ A 
Sbjct: 193 KQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAV 252

Query: 277 ENGG----DHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSS 336
           +  G    D EG+P WLS  DRRLL    S ++AD  VA DGSG FKTVA A+ AAP +S
Sbjct: 253 DIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKTVAAAVAAAPENS 312

Query: 337 GRRIIIYIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGS 396
            +R +I+IKAG Y E + +V +KK N+MF+GDG+  T+I+G ++V D  TTF +AT A  
Sbjct: 313 NKRYVIHIKAGVYRENV-EVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAV 372

Query: 397 GTGIILRDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDI 456
           G   + RD++F+NTAGPSKHQAVALR+ +D +  Y C+++ YQDTLYVHSNRQFF +C I
Sbjct: 373 GERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLI 432

Query: 457 YGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDL 516
            GT+DFIFGNA VV Q C+I+AR+P +GQKN +TAQ R DPNQNTGI I  C+I AT DL
Sbjct: 433 AGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDL 492

Query: 517 EASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPG 576
           ++ KGSFPT+LGRPWK YS+ V M S++ D I P+GW EW G+FAL+TL Y EY N+G G
Sbjct: 493 QSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAG 552

Query: 577 AAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           A    RVKW G++VIT+  EA K+T GQFI G  WL STG  FS GL
Sbjct: 553 AGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of IVF0008943 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 460.7 bits (1184), Expect = 1.8e-129
Identity = 254/579 (43.87%), Postives = 344/579 (59.41%), Query Frame = 0

Query: 36  SSLRRRSRILIISFMSVLLIAASVVSAAILVRSRIASSQPHFPHNKPSQAISRACSHTLY 95
           S  +   ++++ S    LL+ AS+V  A    ++  + +     +     +   CS TLY
Sbjct: 12  SDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLY 71

Query: 96  PSLCLSSLLSFPGAQSADVHDLVHISLNLTLQHLTKALYSTSQIPILQISKDPLAHSAYE 155
           P LC S++ +  G +     +++  SLNLT + +    ++  ++   +    P   +A  
Sbjct: 72  PELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALH 131

Query: 156 DCMELLNDAIDAFSLSL---------FSTGASNHDIMTWLSAALTYHDTCTDGFQ-DVTD 215
           DC+E +++ +D   +++          S      D+ T +S+A+T   TC DGF  D  D
Sbjct: 132 DCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDAD 191

Query: 216 LGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLP-VENRKRRRLMESGTPSSWAAENGG- 275
             V+  +      +  M SN+LA+       D+   E R +     +          G  
Sbjct: 192 RKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDL 251

Query: 276 DHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIIIYI 335
           D +G+P+WLS  DRRLL    S I+AD  VA DGSG F TVA A+ AAP  S +R +I+I
Sbjct: 252 DSDGWPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHI 311

Query: 336 KAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIILRD 395
           KAG Y E + +V +KKTN+MF+GDG+G T+I+G ++V D  TTF +AT A  G   + RD
Sbjct: 312 KAGVYRENV-EVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARD 371

Query: 396 MSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTIDFIF 455
           ++F+NTAGPSKHQAVALR+ +D +  Y+C++  YQDTLYVHSNRQFF +C I GT+DFIF
Sbjct: 372 ITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIF 431

Query: 456 GNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGSFP 515
           GNA  V Q C+I AR+P +GQKN +TAQ R DPNQNTGI I  C+I  T DL A KG+FP
Sbjct: 432 GNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFP 491

Query: 516 TFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVK 575
           T+LGRPWK YSR V M S + D I P+GW EW GSFALDTL Y EY+N G GA    RVK
Sbjct: 492 TYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVK 551

Query: 576 WPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           W GY+VITS  EA  FT GQFI G  WL STG  FS  L
Sbjct: 552 WKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of IVF0008943 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-117
Identity = 225/461 (48.81%), Postives = 294/461 (63.77%), Query Frame = 0

Query: 152 SAYEDCMELLNDAIDAFSLSLF----------STGASNHDIMTWLSAALTYHDTCTDGFQ 211
           +A  DC++LL+ A +  S  +           STG    D+ TW+SAAL+  DTC DGF+
Sbjct: 79  NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138

Query: 212 DVTDLGVKNEVEAKLSDLSEMISNSLAIFSGFGGGDLPVENRKRRRLMESGTPSSWAAEN 271
               + +K  V   LS +   + N L +         P    K + +      +   A +
Sbjct: 139 GTNGI-IKKIVAGGLSKVGTTVRNLLTMVHS------PPSKPKPKPIK---AQTMTKAHS 198

Query: 272 GGDHEGFPEWLSGKDRRLLAAPISAIQADIVVAKDGSGKFKTVAEAIEAAPSSSGRRIII 331
           G     FP W+   DR+LL      + AD VVA DG+G F T+++A+ AAP  S +R +I
Sbjct: 199 G--FSKFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVI 258

Query: 332 YIKAGKYEEEILKVGRKKTNLMFVGDGKGITVISGGKSVYDKVTTFRTATFAGSGTGIIL 391
           ++K G Y E + ++ +KK N+M VGDG   TVI+G +S  D  TTFR+ATFA SG G I 
Sbjct: 259 HVKRGVYVENV-EIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIA 318

Query: 392 RDMSFENTAGPSKHQAVALRLSADHAVVYRCNIIGYQDTLYVHSNRQFFRECDIYGTIDF 451
           RD++F+NTAGP KHQAVA+R   D  V YRC + GYQDTLY HS RQFFREC I GT+DF
Sbjct: 319 RDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDF 378

Query: 452 IFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQNTGISIHACKIVATGDLEASKGS 511
           IFG+A  VFQSC I A++ +  QKN+ITAQ RKDPN+ TG +I    I A  DL  +  +
Sbjct: 379 IFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNT 438

Query: 512 FPTFLGRPWKLYSRVVYMVSSMGDHIHPQGWLEWQGSFALDTLYYGEYMNSGPGAAVGQR 571
             T+LGRPWKLYSR V+M + M D I+P GWLEW G+FALDTLYYGEYMNSGPGA++ +R
Sbjct: 439 TATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRR 498

Query: 572 VKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSFSAGL 603
           VKWPGY V+ ++ EA+ FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 VKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FK058.7e-20162.56Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B05.4e-19557.50Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... [more]
O490064.7e-13043.61Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425342.6e-12843.87Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
O048864.9e-12744.16Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3AUZ60.0e+0099.67Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482964 PE=3 SV=1[more]
A0A0A0KJG10.0e+0096.03Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1[more]
A0A6J1E2V13.4e-29385.45Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1[more]
A0A6J1IA521.0e-28984.38Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1[more]
A0A6J1H2K11.4e-28184.08Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008437596.10.099.67PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_011654619.10.096.03probable pectinesterase/pectinesterase inhibitor 61 [Cucumis sativus] >KGN49865.... [more]
XP_038874841.10.091.07probable pectinesterase/pectinesterase inhibitor 61 [Benincasa hispida][more]
XP_022922302.10.085.81probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata][more]
KAG6579321.10.085.64putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... [more]
Match NameE-valueIdentityDescription
AT5G53370.16.2e-20262.56pectin methylesterase PCR fragment F [more]
AT3G49220.13.9e-19657.50Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G14310.13.3e-13143.61pectin methylesterase 3 [more]
AT1G53830.11.8e-12943.87pectin methylesterase 2 [more]
AT3G43270.11.2e-11748.81Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 81..229
e-value: 1.8E-42
score: 157.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 55..231
e-value: 3.1E-33
score: 112.6
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 85..229
e-value: 4.4E-32
score: 111.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 290..587
e-value: 5.1E-144
score: 478.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 83..233
e-value: 1.6E-37
score: 130.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 84..231
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 284..602
e-value: 2.6E-120
score: 403.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..38
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..36
NoneNo IPR availablePANTHERPTHR31707:SF184PECTINESTERASEcoord: 37..602
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 37..602
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 89..235
e-value: 2.0984E-38
score: 136.799
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 435..444
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 285..601

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008943.2IVF0008943.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity