IVF0008293 (gene) Melon (IVF77) v1

Overview
NameIVF0008293
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionPhosphopyruvate hydratase
Locationchr02: 20946193 .. 20949640 (+)
RNA-Seq ExpressionIVF0008293
SyntenyIVF0008293
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGTGAGCGTCGCTTTCAACATTCTCGTCGCCATTTCCCAACAACCACGCTAACCCCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCCTTTCCTTTGGCGGACTTTCCCACTTTCTTCTTCTTCTTCTTCTATATATACATTCACTCACTCCCCCATTTCCATTTCTCTTCATCAGAACTTCAATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCAACAAACCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGTCTCAATACCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCTACCATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCCACCAACGTCTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGTTGTTGATTCAATTCTTTCTTTATTTGCTTTTATGAGCATTGATTTGAATGTTTGTAGATTGTAATACTGACTTCTAATTGTGATCATTGGAATTTCGTTTTTAGTTCGTTTGATTGTTGACTGTAAATCTGCACCTTTTATAATCTTTGTACATGAGTTTAGACTACAAGTTGCAGAAAGAGACTTGTTTTCAAAAGTTGGGGTCGGTTTTGAATGATTTTCTAATGACTTTTTAAGTGCTTAAAAACATTTTTTTCGCCTAAAACTATTTCTCCAAATATTAGAAACTTGTTCCAAATAGACCCTTCAAATACTTATCTTGATTGTGATTCTTGTGTGTAAAGCTAAAAGGTGGCTTAGTTCAGATTGAGTTTTCAACCTAATTGTTTTGAGACAACGACGTGAATGGAACAGAAAAATGGTGGAAATTTTCGTTAATGGGGATTCTCAAACTATATGGTTTTCTTTTTGCTGTACTTACTGCTCAGAAAAAGTTGGTAGCCTTTCTATTCAATTTATTACTGAAGATGTAAAATTCCAAGCCTTTTCATTGGTTTTGAAAGAGTATAAGGGTATTCAACATAACATGCCCACATAAAGTCTTGCCTAAAGAAAGAGTCTGTCGTTGTCTTTGTGATAGTTGTTTAAGAATAGAATCAATTTTCATTGATGAATGAAATAAGTGGTCAAACTCCCAAACACCTTACAGGATAAGCTTCTAGTTATTGATCAAAGAAGAAAGACTAGAATGAGAAGAAAAAAAGGGAAAAAAAGTGTCTTTGTGGGAGTTGGTTCACTTTTCAGGACTTCTGCTTCCTAACCCAATTTTGTCAACTTCTGTAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATATTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCACTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCCGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGGTTGGCAGCTTCTTCTCAAGTGCTGAAATTTTCTTTTCCATTGTTATGGTGTTAGTTTATGATTCATTTTGCACCTCATAGATATGCTTGTTTCTGACAATATACATGAATTATGGACAGTCATTTCATCTCCGTCATTTTTGTACTTAAACAAGTTATTTGTGAGTGACTTTGGTATTTTCTAATGAACAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGGTACAGGATTGTAAATTTCCCTTATTGTTTTCAAATGGTGTAGCAGTTTTCTTACATGTCCTGCTAAGACAAAAAAAAATTCTTTGATACAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTAAAGGGGGAAAATATGATTTGAATTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCGGCTCATGCCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTAAACAGCCTTTGATTTGTTCTTTCCTTTTTTATCTCTGTTCCAAACTAATTGTAGGATTTAGGTTAGTCCTTAAAAATTTTACTTGTTTGCTGAATGTTTGGTTTTCGATTGATTTAGGTTGACCAATCCCCATATATAGTTAGAAACATGTGATTTTGATGCTGCAGCTTGCTCATATGTTAGAAACTTCTCCTGCAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAGGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCTGTTGGCTTGGCTAGTGGACAGGTTTCATTCATCTTCAGCTCTTCTGCCTATAACTATAAGTCTATAACGCATGTATGTTGTTGACATAATTCACAACTGAATATTGTGTTACTATTATTTTACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTGGCAAAATATAATCAGGTAAAGTTTGACCAAATCTGGACATCCAAAATTATTATCCTTGTATTGCTACAATATTTCTAGTCTTGCTCAATGTATTAATATTTTCATTTTCTTTTTTAATTTTGATGCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAGGAGTTTTAAAAGGTCAAGATCACCTTAGGAGTTAAATAGTTCAAGGTGACGACAGTGTTAATGTATCAATTTTAGGCTTGATAGAACATGTAGACGATTTCTGCAGAAATAATATGCTCAACTTTTCTGTTCAAGGTGTTCGAATCTGATACGTTACGAAGTTCATTCAGTGTTACCATGAGAGTATTTAAATTTTTGTTATGAACTATTCGTTTCAAAATAATGCAG

mRNA sequence

AAGTGAGCGTCGCTTTCAACATTCTCGTCGCCATTTCCCAACAACCACGCTAACCCCTGCTGGCTGCTGCTCTGCTTTGAACGCCCCCTTTCCTTTGGCGGACTTTCCCACTTTCTTCTTCTTCTTCTTCTATATATACATTCACTCACTCCCCCATTTCCATTTCTCTTCATCAGAACTTCAATGGCTTCTCTTCCTTCCCCACCCACCATGAATCTCCTCAACAAACCCCTCTCTCTTTCCCCACCTCAATCCTTCATTGTCTCAATACCCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTCCAAACCCCTTCGTTCCCTACCATTCGCTGTTCTGTTGCTGTGGCTCCTTCTGTTTCCACCAACGTCTCCAAGGAATTCAAGTTGAAGTCCCTCAAGGCCCGTCAGATCATTGACAGTAGAGGCAATCCTACGGTCGAGGTTGATTTGATTACTGACGATCTCTACCGATCCGCTGTTCCTAGTGGGGCTTCTACTGGGATCTATGAGGCGTTGGAGCTTAGAGATGGGGACAAAACTGTTTATGGAGGTAAAGGTGTGCTTACTGCTGTTAAGAATATCAATGATATCTTGGCTCCTAAGCTCGTTGGCGTCGATGTCAGGAATCAAGAAGAAGTTGATGCAATCATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATATTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCACTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCCGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTAAAGGGGGAAAATATGATTTGAATTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCGGCTCATGCCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAGGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCTGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTGGCAAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAGGAGTTTTAAAAGGTCAAGATCACCTTAGGAGTTAAATAGTTCAAGGTGACGACAGTGTTAATGTATCAATTTTAGGCTTGATAGAACATGTAGACGATTTCTGCAGAAATAATATGCTCAACTTTTCTGTTCAAGGTGTTCGAATCTGATACGTTACGAAGTTCATTCAGTGTTACCATGAGAGTATTTAAATTTTTGTTATGAACTATTCGTTTCAAAATAATGCAG

Coding sequence (CDS)

ATGCTGGAAATTGATGGAACCCCAAACAAGTCGAAACTTGGGGCAAATGCTATATTAGGAGTTTCTTTGAGTGTCTGCAGAGCTGGTGCTGGCGCTAAGGGACTACCACTGTATAGACACATCCAAGAATTATCAGGCACCAAGGAACTTGTTATGCCAGTTCCTGCTTTTAATGTTATAAACGGGGGTAGCCATGCCGGAAATAATCTAGCAATGCAAGAATTTATGATCTTACCTGTAGGTGCTTCATCTTTCGCAGAGGCACTTCGGATGGGAAGTGAAGTTTATCACACACTGAAGGGAATTATTAAAGCAAAATATGGACAAGATGCCTGCAATGTTGGAGATGAGGGTGGATTCGCTCCAAATGTTCAAGATAACAGAGAAGGACTGGTGTTGCTTATTGATGCCATTGAAAAGGCTGGCTATACAGGAAAGATCAAAATAGGAATGGATGTTGCAGCTTCCGAGTTTTTCACTAAAGGGGGAAAATATGATTTGAATTTCAAGAAACAGCCAAATGATGGAGCTCATGTTCACTCGGCTCATGCCCTTGGCGAACTCTATAAACAGTTTGTCAAAGATTTTCCCATTGTTTCCATCGAGGACCCCTTTGATCAAGATGATTGGAGTTCCTGGGCTTCTCTACAATCTTCTGTTGATATCCAACTTGTGGGAGATGATTTGCTGGTAACAAATCCAAAGAGAATTGCCGAAGGCATTCAGAAAAAAGCTTGTAATGCTTTACTTCTGAAGGTTAACCAAATTGGATCAGTGACTGAATCAATTCAGGCTGCTCTTGATTCCAAGGCTGCTGGCTGGGGAGTGATGGTCAGCCACCGAAGTGGTGAAACTGAAGATAATTTTATTGCGGATCTTTCTGTTGGCTTGGCTAGTGGACAGATCAAAACTGGAGCTCCATGCCGAAGCGAACGGTTGGCAAAATATAATCAGCTTCTTCGCATTGAAGAAGAACTTGGCAGTGTTCGATATGCCGGTGCGTCTTTCAGATCACCTTAG

Protein sequence

MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVINGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVHSAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Homology
BLAST of IVF0008293 vs. ExPASy Swiss-Prot
Match: Q9C9C4 (Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 1.8e-179
Identity = 309/339 (91.15%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLE+DGTPNKSKLGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 139 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 198

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 199 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 258

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF K G+YDLNFKKQPNDGAHV 
Sbjct: 259 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 318

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SA +L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 319 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 378

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 379 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 438

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 439 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of IVF0008293 vs. ExPASy Swiss-Prot
Match: P15429 (Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3)

HSP 1 Score: 498.8 bits (1283), Expect = 4.8e-140
Identity = 247/340 (72.65%), Postives = 286/340 (84.12%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           M+E+DGT NKSK GANAILGVSL+VC+AGA  KG+PLYRHI +L+G  +LV+PVPAFNVI
Sbjct: 94  MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLVLPVPAFNVI 153

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGN LAMQEFMILPVGASSF EA+R+G+EVYH LKG+IKAKYG+DA NVGDEGGF
Sbjct: 154 NGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGF 213

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APN+ +N E L LL  AI+ AGY  K+ IGMDVAASEF+ + GKYDL+F K P+D A   
Sbjct: 214 APNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFY-RNGKYDLDF-KSPDDPARHI 273

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           S   LGELYK F+K++P+VSIEDPFDQDDW++W S  S VDIQ+VGDDL VTNPKRIA+ 
Sbjct: 274 SGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVDIQIVGDDLTVTNPKRIAQA 333

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           ++KKACN LLLKVNQIGSVTESIQA   +++ GWGVMVSHRSGETED FIADL VGL +G
Sbjct: 334 VEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTG 393

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELG-SVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQL+RIEE LG    +AG  FR+P
Sbjct: 394 QIKTGAPCRSERLAKYNQLMRIEEALGDKAVFAGRKFRNP 431

BLAST of IVF0008293 vs. ExPASy Swiss-Prot
Match: Q54RK5 (Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 8.2e-140
Identity = 249/338 (73.67%), Postives = 286/338 (84.62%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           M+++DGTPNK KLGANAIL VSL+VCRAGA  + LPLY++I E++GTK + +PVPAFNVI
Sbjct: 97  MIDLDGTPNKGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTK-MRLPVPAFNVI 156

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGN LAMQEFMILPVGA  F EA RMGSEVYH LK +I  +YGQDA NVGDEGGF
Sbjct: 157 NGGSHAGNKLAMQEFMILPVGAKDFNEAYRMGSEVYHNLKNVISGRYGQDAINVGDEGGF 216

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           AP +Q N+EGL LL  AIEKAGYTG +KIGMD AASEF  + G YDL+FK + NDG+ V 
Sbjct: 217 APPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFKVENG-YDLDFKTKNNDGSAVI 276

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           S   LG+LY++F+K++PI+SIEDPFDQDDW S+  L +SVDIQ+VGDDLLVTNP+RI  G
Sbjct: 277 SGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVGDDLLVTNPERIKTG 336

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           I+KKACNALLLKVNQIGSVTESI+AALDSK A WGVMVSHRSGETED FIADL VGL +G
Sbjct: 337 IEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETEDTFIADLVVGLGTG 396

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELG-SVRYAGASFR 338
           QIKTGAPCRSERLAKYNQL+RI EELG +  YAG +FR
Sbjct: 397 QIKTGAPCRSERLAKYNQLVRINEELGENHNYAGLTFR 432

BLAST of IVF0008293 vs. ExPASy Swiss-Prot
Match: Q3ZC09 (Beta-enolase OS=Bos taurus OX=9913 GN=ENO3 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-139
Identity = 246/340 (72.35%), Postives = 286/340 (84.12%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           M+E+DGT NKSK GANAILGVSL+VC+AGA  KG+PLYRHI +L+G  EL++PVPAFNVI
Sbjct: 94  MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPELILPVPAFNVI 153

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGN LAMQEFMILPVGASSF EA+R+G+EVYH LKG+IKAKYG+DA NVGDEGGF
Sbjct: 154 NGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGF 213

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APN+ +N E L LL  AI+ AGY  K+ IGMDVAASEF+ + GKYDL+F K P+D A   
Sbjct: 214 APNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFY-RNGKYDLDF-KSPDDPARHI 273

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           S   LGELYK F+K++P+VSIEDPFDQDDW++W S  S V+IQ+VGDDL VTNPKRIA+ 
Sbjct: 274 SGEKLGELYKNFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQA 333

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           ++KKACN LLLKVNQIGSVTESIQA   +++ GWGVMVSHRSGETED FIADL VGL +G
Sbjct: 334 VEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTG 393

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELG-SVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQL+RIEE LG    +AG  FR+P
Sbjct: 394 QIKTGAPCRSERLAKYNQLMRIEEALGDKAVFAGRKFRNP 431

BLAST of IVF0008293 vs. ExPASy Swiss-Prot
Match: P42896 (Enolase OS=Ricinus communis OX=3988 PE=2 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 1.1e-139
Identity = 250/345 (72.46%), Postives = 285/345 (82.61%), Query Frame = 0

Query: 3   EIDGTPN-----KSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAF 62
           E+DGT N     K KLGANAIL VSL++C+AGA  KG+PLY+HI  L+G K LV+PVPAF
Sbjct: 97  ELDGTVNEWGWCKQKLGANAILAVSLALCKAGAHVKGIPLYKHIANLAGNKNLVLPVPAF 156

Query: 63  NVINGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDE 122
           NVINGGSHAGN LAMQEFMILPVGASSF EA++MG+EVYH LK +IK KYGQDA NVGDE
Sbjct: 157 NVINGGSHAGNKLAMQEFMILPVGASSFKEAMKMGAEVYHHLKSVIKKKYGQDATNVGDE 216

Query: 123 GGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGA 182
           GGFAPN+Q+N+EGL LL  AI KAGYTGK+ IGMDVAASEF+     YDLNFK++ NDG+
Sbjct: 217 GGFAPNIQENKEGLELLKTAIAKAGYTGKVVIGMDVAASEFYGSDKTYDLNFKEENNDGS 276

Query: 183 HVHSAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSV--DIQLVGDDLLVTNPK 242
              S  AL +LYK F  ++PIVSIEDPFDQDDW  ++ L S +   +Q+VGDDLLVTNPK
Sbjct: 277 QKISGEALKDLYKSFASEYPIVSIEDPFDQDDWEHYSKLTSEIGEKVQIVGDDLLVTNPK 336

Query: 243 RIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSV 302
           R+ + IQ+KACNALLLKVNQIGSVTESI+A   SK AGWGVM SHRSGETED FIADLSV
Sbjct: 337 RVEKAIQEKACNALLLKVNQIGSVTESIEAVRMSKRAGWGVMASHRSGETEDTFIADLSV 396

Query: 303 GLASGQIKTGAPCRSERLAKYNQLLRIEEELGS-VRYAGASFRSP 340
           GLA+GQIKTGAPCRSERLAKYNQLLRIEEELG+   YAGA FR+P
Sbjct: 397 GLATGQIKTGAPCRSERLAKYNQLLRIEEELGAEAVYAGAKFRTP 441

BLAST of IVF0008293 vs. ExPASy TrEMBL
Match: A0A5D3DYS6 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00060 PE=3 SV=1)

HSP 1 Score: 677.6 bits (1747), Expect = 2.8e-191
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 338 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 496

BLAST of IVF0008293 vs. ExPASy TrEMBL
Match: A0A5A7US84 (Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G003390 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.3e-190
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 338 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 496

BLAST of IVF0008293 vs. ExPASy TrEMBL
Match: A0A1S3BX56 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.3e-190
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 338 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 496

BLAST of IVF0008293 vs. ExPASy TrEMBL
Match: A0A0A0LPK6 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 9.9e-189
Identity = 335/339 (98.82%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKS+LGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 154 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 213

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 214 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 273

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVH
Sbjct: 274 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 333

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAH LGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 334 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 393

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 394 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 453

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 454 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 492

BLAST of IVF0008293 vs. ExPASy TrEMBL
Match: A0A6J1BZW1 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 5.1e-185
Identity = 328/339 (96.76%), Postives = 331/339 (97.64%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGT NKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGA+SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAH LGELYKQF+KDFPIVSIEDPFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 338 SAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEA 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           I KKAC ALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IHKKACTALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAG SFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGESFRSP 496

BLAST of IVF0008293 vs. NCBI nr
Match: TYK28380.1 (enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 672 bits (1734), Expect = 4.67e-241
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 338 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 339
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 496

BLAST of IVF0008293 vs. NCBI nr
Match: XP_008453338.1 (PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 669 bits (1726), Expect = 7.71e-240
Identity = 338/339 (99.71%), Postives = 338/339 (99.71%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 338 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 339
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 496

BLAST of IVF0008293 vs. NCBI nr
Match: XP_004138202.1 (enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_014309 [Cucumis sativus])

HSP 1 Score: 664 bits (1712), Expect = 9.02e-238
Identity = 335/339 (98.82%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKS+LGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 154 MLEIDGTPNKSELGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 213

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 214 NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 273

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFT  GKYDLNFKKQPNDGAHVH
Sbjct: 274 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTAEGKYDLNFKKQPNDGAHVH 333

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAH LGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG
Sbjct: 334 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 393

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 394 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 453

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 339
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP
Sbjct: 454 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 492

BLAST of IVF0008293 vs. NCBI nr
Match: XP_038879680.1 (enolase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 661 bits (1706), Expect = 6.87e-237
Identity = 332/339 (97.94%), Postives = 336/339 (99.12%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 152 MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 211

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGA SFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF
Sbjct: 212 NGGSHAGNNLAMQEFMILPVGAISFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 271

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDN+EGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 272 APNVQDNKEGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKEGKYDLNFKKQPNDGAHVH 331

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAH LGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIA+G
Sbjct: 332 SAHGLGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAKG 391

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 392 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 451

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 339
           QIKTGAPCRSERLAKYNQLLRIEEE+GS+RYAGASFRSP
Sbjct: 452 QIKTGAPCRSERLAKYNQLLRIEEEIGSIRYAGASFRSP 490

BLAST of IVF0008293 vs. NCBI nr
Match: XP_022134829.1 (enolase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 651 bits (1680), Expect = 7.74e-233
Identity = 328/339 (96.76%), Postives = 331/339 (97.64%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLEIDGT NKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI
Sbjct: 158 MLEIDGTTNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 217

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGNNLAMQEFMILPVGA+SFAEALRMGSEVYH LKGIIKAKYGQDACNVGDEGGF
Sbjct: 218 NGGSHAGNNLAMQEFMILPVGATSFAEALRMGSEVYHILKGIIKAKYGQDACNVGDEGGF 277

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTK GKYDLNFKKQPNDGAHVH
Sbjct: 278 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKDGKYDLNFKKQPNDGAHVH 337

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SAH LGELYKQF+KDFPIVSIEDPFDQDDWSSW+SLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 338 SAHGLGELYKQFIKDFPIVSIEDPFDQDDWSSWSSLQSSVDIQLVGDDLLVTNPKRIAEA 397

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           I KKAC ALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 398 IHKKACTALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 457

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 339
           QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAG SFRSP
Sbjct: 458 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGESFRSP 496

BLAST of IVF0008293 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 629.8 bits (1623), Expect = 1.3e-180
Identity = 309/339 (91.15%), Postives = 329/339 (97.05%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           MLE+DGTPNKSKLGANAILGVSLSVCRAGAGAKG+PLY+HIQE SGTKELVMPVPAFNVI
Sbjct: 139 MLELDGTPNKSKLGANAILGVSLSVCRAGAGAKGVPLYKHIQETSGTKELVMPVPAFNVI 198

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           NGGSHAGN+LAMQEFMILPVGA+SF+EA +MGSEVYHTLKGIIK KYGQDACNVGDEGGF
Sbjct: 199 NGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSEVYHTLKGIIKTKYGQDACNVGDEGGF 258

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFF K G+YDLNFKKQPNDGAHV 
Sbjct: 259 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVL 318

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SA +L +LY++F+KDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAE 
Sbjct: 319 SAESLADLYREFIKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEA 378

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           I+K++CNALLLKVNQIG+VTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG
Sbjct: 379 IKKQSCNALLLKVNQIGTVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 438

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELGSVRYAGASFRSP 340
           QIKTGAPCRSERL+KYNQLLRIEEELG+VRYAG +FRSP
Sbjct: 439 QIKTGAPCRSERLSKYNQLLRIEEELGNVRYAGEAFRSP 477

BLAST of IVF0008293 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 490.0 bits (1260), Expect = 1.6e-138
Identity = 244/345 (70.72%), Postives = 286/345 (82.90%), Query Frame = 0

Query: 3   EIDGTPN-----KSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAF 62
           E+DGT N     K KLGANAIL VSL+VC+AGA   G+PLY+HI  L+G  ++V+PVPAF
Sbjct: 96  ELDGTQNEWGWCKQKLGANAILAVSLAVCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAF 155

Query: 63  NVINGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDE 122
           NVINGGSHAGN LAMQEFMILPVGA+SF EA++MG EVYH LK +IK KYGQDA NVGDE
Sbjct: 156 NVINGGSHAGNKLAMQEFMILPVGAASFKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDE 215

Query: 123 GGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGA 182
           GGFAPN+Q+N+EGL LL  AIEKAGYTGK+ IGMDVAASEF+++   YDLNFK++ N+G+
Sbjct: 216 GGFAPNIQENKEGLELLKTAIEKAGYTGKVVIGMDVAASEFYSEDKTYDLNFKEENNNGS 275

Query: 183 HVHSAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASL--QSSVDIQLVGDDLLVTNPK 242
              S  AL +LYK FV ++PIVSIEDPFDQDDW  +A +  +   ++Q+VGDDLLVTNPK
Sbjct: 276 QKISGDALKDLYKSFVAEYPIVSIEDPFDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPK 335

Query: 243 RIAEGIQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSV 302
           R+A+ I +K+CNALLLKVNQIGSVTESI+A   SK AGWGVM SHRSGETED FIADL+V
Sbjct: 336 RVAKAIAEKSCNALLLKVNQIGSVTESIEAVKMSKKAGWGVMTSHRSGETEDTFIADLAV 395

Query: 303 GLASGQIKTGAPCRSERLAKYNQLLRIEEELGS-VRYAGASFRSP 340
           GL++GQIKTGAPCRSERLAKYNQLLRIEEELGS   YAG +FR P
Sbjct: 396 GLSTGQIKTGAPCRSERLAKYNQLLRIEEELGSEAIYAGVNFRKP 440

BLAST of IVF0008293 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 374.0 bits (959), Expect = 1.3e-103
Identity = 185/338 (54.73%), Postives = 241/338 (71.30%), Query Frame = 0

Query: 1   MLEIDGTPNKSKLGANAILGVSLSVCRAGAGAKGLPLYRHIQELSGTKELVMPVPAFNVI 60
           M+++D T  KS+LGANAIL VS++ C+AGA  K +PL +H+ +LSG   +V+PVPAF V+
Sbjct: 136 MIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLSGRANMVLPVPAFTVL 195

Query: 61  NGGSHAGNNLAMQEFMILPVGASSFAEALRMGSEVYHTLKGIIKAKYGQDACNVGDEGGF 120
           +GG HA N  A+QE MILP+GAS F EAL+ GSE YH LK +I  K G   CNVG++GG 
Sbjct: 196 SGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISEKNGGLGCNVGEDGGL 255

Query: 121 APNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVAASEFFTKGGKYDLNFKKQPNDGAHVH 180
           AP++   +EGL L+ +AI + GY  KIKI +D+AA+  F  G KYDL+ K     G +  
Sbjct: 256 APDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATN-FCLGTKYDLDIKSPNKSGQNFK 315

Query: 181 SAHALGELYKQFVKDFPIVSIEDPFDQDDWSSWASLQSSVDIQLVGDDLLVTNPKRIAEG 240
           SA  + ++YK+   D+PIVSIEDPFD++DW       S    Q+VGDDLL++N KR+   
Sbjct: 316 SAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVGDDLLMSNSKRVERA 375

Query: 241 IQKKACNALLLKVNQIGSVTESIQAALDSKAAGWGVMVSHRSGETEDNFIADLSVGLASG 300
           IQ+ +CNALLLKVNQIG+VTE+I+    ++ A WGV+ SHR GETED+FI+DLSVGLA+G
Sbjct: 376 IQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETEDSFISDLSVGLATG 435

Query: 301 QIKTGAPCRSERLAKYNQLLRIEEELG-SVRYAGASFR 338
            IK GAPCR ER  KYNQLLRIEEELG    YAG  ++
Sbjct: 436 VIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C9C41.8e-17991.15Enolase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ENO1 PE=1 SV=1[more]
P154294.8e-14072.65Beta-enolase OS=Rattus norvegicus OX=10116 GN=Eno3 PE=1 SV=3[more]
Q54RK58.2e-14073.67Enolase A OS=Dictyostelium discoideum OX=44689 GN=enoA PE=3 SV=1[more]
Q3ZC091.1e-13972.35Beta-enolase OS=Bos taurus OX=9913 GN=ENO3 PE=2 SV=1[more]
P428961.1e-13972.46Enolase OS=Ricinus communis OX=3988 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3DYS62.8e-191100.00Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7US842.3e-19099.71Phosphopyruvate hydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
A0A1S3BX562.3e-19099.71Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103494088 PE=3 SV=1[more]
A0A0A0LPK69.9e-18998.82Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G014400 PE=3 SV=1[more]
A0A6J1BZW15.1e-18596.76Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111007005 PE=3 SV... [more]
Match NameE-valueIdentityDescription
TYK28380.14.67e-241100.00enolase 1 [Cucumis melo var. makuwa][more]
XP_008453338.17.71e-24099.71PREDICTED: enolase 1, chloroplastic [Cucumis melo] >KAA0058034.1 enolase 1 [Cucu... [more]
XP_004138202.19.02e-23898.82enolase 1, chloroplastic [Cucumis sativus] >KGN63743.1 hypothetical protein Csa_... [more]
XP_038879680.16.87e-23797.94enolase 1, chloroplastic [Benincasa hispida][more]
XP_022134829.17.74e-23396.76enolase 1, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G74030.11.3e-18091.15enolase 1 [more]
AT2G36530.11.6e-13870.72Enolase [more]
AT2G29560.11.3e-10354.73cytosolic enolase [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000941EnolasePRINTSPR00148ENOLASEcoord: 249..263
score: 80.2
coord: 226..237
score: 78.16
coord: 71..84
score: 77.06
coord: 14..30
score: 76.74
coord: 278..295
score: 83.0
IPR000941EnolasePANTHERPTHR11902ENOLASEcoord: 1..339
IPR000941EnolaseCDDcd03313enolasecoord: 1..325
e-value: 0.0
score: 587.912
IPR020811Enolase, N-terminalSMARTSM01193Enolase_N_3coord: 1..41
e-value: 0.0014
score: -24.0
IPR020811Enolase, N-terminalPFAMPF03952Enolase_Ncoord: 1..41
e-value: 1.1E-14
score: 54.7
IPR020810Enolase, C-terminal TIM barrel domainSMARTSM01192Enolase_C_3coord: 49..339
e-value: 3.2E-193
score: 657.9
IPR020810Enolase, C-terminal TIM barrel domainPFAMPF00113Enolase_Ccoord: 50..337
e-value: 1.2E-147
score: 491.0
IPR029017Enolase-like, N-terminalGENE3D3.30.390.10coord: 1..34
e-value: 5.9E-11
score: 44.6
IPR029017Enolase-like, N-terminalSUPERFAMILY54826Enolase N-terminal domain-likecoord: 1..47
IPR036849Enolase-like, C-terminal domain superfamilyGENE3D3.20.20.120coord: 35..339
e-value: 6.0E-153
score: 510.4
IPR036849Enolase-like, C-terminal domain superfamilySUPERFAMILY51604Enolase C-terminal domain-likecoord: 49..333
NoneNo IPR availableSFLDSFLDG00178enolasecoord: 1..326
e-value: 0.0
score: 493.5
NoneNo IPR availableSFLDSFLDS00001Enolasecoord: 1..326
e-value: 0.0
score: 493.5
NoneNo IPR availablePANTHERPTHR11902:SF42ENOLASE 1, CHLOROPLASTICcoord: 1..339
IPR020809Enolase, conserved sitePROSITEPS00164ENOLASEcoord: 249..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008293.1IVF0008293.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006096 glycolytic process
biological_process GO:0010090 trichome morphogenesis
cellular_component GO:0009507 chloroplast
cellular_component GO:0000015 phosphopyruvate hydratase complex
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0004634 phosphopyruvate hydratase activity