IVF0008195 (gene) Melon (IVF77) v1

Overview
NameIVF0008195
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionGDSL esterase/lipase
Locationchr08: 7599713 .. 7601417 (+)
RNA-Seq ExpressionIVF0008195
SyntenyIVF0008195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCATTTCATTATATAATATAAATAGGCCGTTATAGCATTGCACATTATTAAACACAATTCCCCTATGCATTGCATATATATGAGCTTATAATAACATTAATTATCATTCAGTAATGGACTTTAATCTGAAGCTTCTCTTCACTTTCTTCTTCTTCACGGTCTCCCTCTTTTCAGGTTGGTACTTGGTTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATTTGAATTGATGAATGAATCATCCCATGAGGTTAATTTGACGTAAAACAGGACAAGGATCCGCCGGTGGGTATTACAGTAACCGCCACCACCGGAGCCACCGTTTACGGGCGACGAAACTATTTGTTTTTGGAGATTCATATGTTGATACCGGCAACATTCTGGTTCCGTTTTCAAGTGCTCAACAATTTCCTTATGGAATCACTTTCCCCGGAAAACCCAGTGGTCGGTTTTCGGATGGTCGTGTTCTGACTGATTTTGCAGGGAAACATTTGGGGGTGAAATCTCCGATACCATTCAGTATACGAAATGAAGTTGGGGAGGAGAGATTGAAAGAATCGGGGATAAATTTTGCATTTGGTGGAACAGGGGTTTTCGACACGTCGGTTCCTCTCCCAAATATGACAACACAAATTGACTTGTTTGAACAACTTCGACATGCCGAATCTGGGTTGAGTAATAGAGATGTTCATCTCTCTGTTGCACTTGTCTCTGTTTCTGGGAACGATTACTCTTTTTATCTTGCAACTAATGGCTCTGCCCAGGTAATTCATTCATTATTTTTATCCTCTCTTAGTGATTATGTTTATGTTTGTTTATCTTTTAGAATCTCTGTTTACACTGAAAAGGAGAACGAGAAGTGGACAGAAATAGAGAATAAAATGGATATAATCCACCCCATTCCACATAATCTATACCCATATAGAGAATTTAAATGGGGGGCGTGGACAAGGACGAATATTTGTTGTTACAATTTATTAGATAATATAAAGTAAGAATTGAAAGTGAGATTCTTCATATATCTATTGGAGATGATGTCTTTTTGGGCTTTCCAAATTTTTATTTCTACATTTTTTGATGACGTGGTGAGTTAGTTAACTTGTTTGATATTTACTCGTTGTTTTACAGGGATTAAAACCATTCATAAACTCAGTAGTGAATCAAGTAATGATAGATTTGAAGAGGATTAAGAGGTTAGGAGTAAAGAAAATAGTAGTGACGGGGCTAGGGCCATTGGGATGTCTTCCAATATTTACAGCTCCATTCTCATTCAAGCAATGCAATCAAACCATAAACTCATTTGTCCAATTTCACAACTTTTTGTTGAAGCAAGCCGTGGATAAACTCAACAAACAAATTACTAACCAGCAACATTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAAAATCTTCATTCTTGATGTGTATGATGCATTCTTGTCCTTGATCCAAGGCCGTGGCAGTGGCAGAGTTGGGTTATTAAAGTTCAAGACCCCATTAAAGCCATGTTGTTTTGGGGTGAGCAGTGGATTTAACTGTGGGAGTGTGGATGAAAAAGGGAATAAAAAATTTGTGTTATGTAATGATCCCAAATCAGCTTTCTTTTGGGATTCAGTTCATCCTACTCAAACAGGATGGTCTCTTGCATTTTCCTCTTTTACATCAATTCTCTAA

mRNA sequence

TTTTCATTTCATTATATAATATAAATAGGCCGTTATAGCATTGCACATTATTAAACACAATTCCCCTATGCATTGCATATATATGAGCTTATAATAACATTAATTATCATTCAGTAATGGACTTTAATCTGAAGCTTCTCTTCACTTTCTTCTTCTTCACGGTCTCCCTCTTTTCAGGACAAGGATCCGCCGGTGGGTATTACAGTAACCGCCACCACCGGAGCCACCGTTTACGGGCGACGAAACTATTTGTTTTTGGAGATTCATATGTTGATACCGGCAACATTCTGGTTCCGTTTTCAAGTGCTCAACAATTTCCTTATGGAATCACTTTCCCCGGAAAACCCAGTGGTCGGTTTTCGGATGGTCGTGTTCTGACTGATTTTGCAGGGAAACATTTGGGGGTGAAATCTCCGATACCATTCAGTATACGAAATGAAGTTGGGGAGGAGAGATTGAAAGAATCGGGGATAAATTTTGCATTTGGTGGAACAGGGGTTTTCGACACGTCGGTTCCTCTCCCAAATATGACAACACAAATTGACTTGTTTGAACAACTTCGACATGCCGAATCTGGGTTGAGTAATAGAGATGTTCATCTCTCTGTTGCACTTGTCTCTGTTTCTGGGAACGATTACTCTTTTTATCTTGCAACTAATGGCTCTGCCCAGGGATTAAAACCATTCATAAACTCAGTAGTGAATCAAGTAATGATAGATTTGAAGAGGATTAAGAGGTTAGGAGTAAAGAAAATAGTAGTGACGGGGCTAGGGCCATTGGGATGTCTTCCAATATTTACAGCTCCATTCTCATTCAAGCAATGCAATCAAACCATAAACTCATTTGTCCAATTTCACAACTTTTTGTTGAAGCAAGCCGTGGATAAACTCAACAAACAAATTACTAACCAGCAACATTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAAAATCTTCATTCTTGATGTGTATGATGCATTCTTGTCCTTGATCCAAGGCCGTGGCAGTGGCAGACCATGTTGTTTTGGGGTGAGCAGTGGATTTAACTGTGGGAGTGTGGATGAAAAAGGGAATAAAAAATTTGTGTTATGTAATGATCCCAAATCAGCTTTCTTTTGGGATTCAGTTCATCCTACTCAAACAGGATGGTCTCTTGCATTTTCCTCTTTTACATCAATTCTCTAA

Coding sequence (CDS)

ATGGACTTTAATCTGAAGCTTCTCTTCACTTTCTTCTTCTTCACGGTCTCCCTCTTTTCAGGACAAGGATCCGCCGGTGGGTATTACAGTAACCGCCACCACCGGAGCCACCGTTTACGGGCGACGAAACTATTTGTTTTTGGAGATTCATATGTTGATACCGGCAACATTCTGGTTCCGTTTTCAAGTGCTCAACAATTTCCTTATGGAATCACTTTCCCCGGAAAACCCAGTGGTCGGTTTTCGGATGGTCGTGTTCTGACTGATTTTGCAGGGAAACATTTGGGGGTGAAATCTCCGATACCATTCAGTATACGAAATGAAGTTGGGGAGGAGAGATTGAAAGAATCGGGGATAAATTTTGCATTTGGTGGAACAGGGGTTTTCGACACGTCGGTTCCTCTCCCAAATATGACAACACAAATTGACTTGTTTGAACAACTTCGACATGCCGAATCTGGGTTGAGTAATAGAGATGTTCATCTCTCTGTTGCACTTGTCTCTGTTTCTGGGAACGATTACTCTTTTTATCTTGCAACTAATGGCTCTGCCCAGGGATTAAAACCATTCATAAACTCAGTAGTGAATCAAGTAATGATAGATTTGAAGAGGATTAAGAGGTTAGGAGTAAAGAAAATAGTAGTGACGGGGCTAGGGCCATTGGGATGTCTTCCAATATTTACAGCTCCATTCTCATTCAAGCAATGCAATCAAACCATAAACTCATTTGTCCAATTTCACAACTTTTTGTTGAAGCAAGCCGTGGATAAACTCAACAAACAAATTACTAACCAGCAACATTCTTATTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTAAAATCTTCATTCTTGATGTGTATGATGCATTCTTGTCCTTGATCCAAGGCCGTGGCAGTGGCAGACCATGTTGTTTTGGGGTGAGCAGTGGATTTAACTGTGGGAGTGTGGATGAAAAAGGGAATAAAAAATTTGTGTTATGTAATGATCCCAAATCAGCTTTCTTTTGGGATTCAGTTCATCCTACTCAAACAGGATGGTCTCTTGCATTTTCCTCTTTTACATCAATTCTCTAA

Protein sequence

MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGRPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL
Homology
BLAST of IVF0008195 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.2e-94
Identity = 183/364 (50.27%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 1   MDFNLKLLF-TFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILV 60
           MD  +KL F  F F   SL  G+ + G   SN++H  +  R TKLFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEIN-GVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  PFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGI 120
            FSS+ +FPYGITFPGKP+GRFSDGRV TDF  K +G+KSPIP+  ++  G++RL + G+
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGM 120

Query: 121 NFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLA 180
           NFA+GGTGVF+T  PLPNMTTQID+F+ +          ++  SVALVSV+GNDYS ++A
Sbjct: 121 NFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIA 180

Query: 181 TNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQT 240
            N  A     FI  VV+Q  ++L+RI  LGVKKI V  L PLGCLP FT   SF++CN+T
Sbjct: 181 LNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNET 240

Query: 241 INSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR 300
            N+ V  HN LL+Q V KLN +                +  S   ILD+Y+AFL++ + +
Sbjct: 241 QNALVNLHNNLLQQVVAKLNNE----------------TKQSTFIILDLYNAFLTVFKNK 300

Query: 301 GSG----------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 354
           GS           +PCC GVS  +NCGSVDEKG KK+++C++PK+AFFWD +HPT+ GW 
Sbjct: 301 GSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWR 346

BLAST of IVF0008195 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.3e-83
Identity = 164/359 (45.68%), Postives = 226/359 (62.95%), Query Frame = 0

Query: 6   KLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQ 65
           KLL + F F+ S F   G+   Y   +H    RLR  +LFVFGDSY DTGNI    S + 
Sbjct: 5   KLLISLFLFSFSSFF-LGAESDY--PQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSW 64

Query: 66  QFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGG 125
           + PYGITFP KPSGRFSDGRV TDF  ++LG+KSPIP++ ++  G+ERL   G+N+A+GG
Sbjct: 65  KIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGG 124

Query: 126 TGVFDT-SVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSA 185
           TGVF T   PLPNMTTQID F+++  A +  S  D+  S+ALVSV+GNDY+ +LA     
Sbjct: 125 TGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPL 184

Query: 186 QGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFV 245
             L  F+  VV+Q+ ++  RI +LGV KIV+  + PLGCLP  T   SF++CN T N+  
Sbjct: 185 TELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAST 244

Query: 246 QFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG-- 305
             HN+LL +A+ +LN +                +  S   +LD Y+AFL++ + +G    
Sbjct: 245 NLHNYLLHKAIARLNNE----------------TKPSTFVVLDHYNAFLTVFKNKGPEPG 304

Query: 306 --------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFS 354
                   +PCC GV+S ++C +VDEKG KK+++C DPK+AFFWD  HP++ GW   +S
Sbjct: 305 VSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of IVF0008195 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 2.1e-61
Identity = 142/350 (40.57%), Postives = 203/350 (58.00%), Query Frame = 0

Query: 13  FFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGIT 72
           FF++SLF G   A G  SN+  +++  +  KLFVFG+SY DTGN + P + + + PYGIT
Sbjct: 8   FFSLSLFFGINGAVG--SNQPSQAN--KNPKLFVFGNSYADTGN-MKPTALSWKLPYGIT 67

Query: 73  FPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLK-ESGINFAFGGTGVFDT 132
           FPGKPSGR+SDG   TDF  K LG K  +P+  R   G++++K   G+NFAFGG+ VFD+
Sbjct: 68  FPGKPSGRYSDGLTATDFLAKQLGAK--LPYLWRTH-GKKKVKLNRGMNFAFGGSEVFDS 127

Query: 133 SVP-LPNMTTQIDLFEQLRHAESGLS-NRDVHLSVALVSVSGNDYSFYLATNGSAQGLKP 192
            V   PN++TQ+     L  A    + + D+  S AL+S SG DY  ++  N +      
Sbjct: 128 PVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYPA 187

Query: 193 FINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNF 252
           F+  +V  +   L  +  L  K I VT L PLGCLP  T   SF+ CN++ +  V+ HN 
Sbjct: 188 FVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHNE 247

Query: 253 LLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR------ 312
            LK+AV KLNK+                +   +  I+D++ AF+++++ +G+ R      
Sbjct: 248 SLKKAVAKLNKE------------DKFRTKGDRFVIVDLHKAFMTILEKKGNKRFKSPLK 307

Query: 313 PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFS 354
           PCC G     +C  +D KG KK+ LCNDPKSAFFWD ++PTQ GW   +S
Sbjct: 308 PCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYS 332

BLAST of IVF0008195 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.8e-60
Identity = 133/323 (41.18%), Postives = 188/323 (58.20%), Query Frame = 0

Query: 40  RATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKS 99
           +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG +S
Sbjct: 11  KVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 70

Query: 100 PIPFSIRNEVGEERLKESGINFAFGGTGVFDTS--VPLPNMTTQIDLFEQLRHAESGLSN 159
           P  +      G ++  + G+NFAFGG+ + D+S   P PN+T Q++    L    +G   
Sbjct: 71  PYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGRVY 130

Query: 160 RDVHLS-VALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVT 219
            D+  S V+L+S +G DY +Y+  N  A GLK  +  VV+ + +++  +  L  KKI VT
Sbjct: 131 GDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVT 190

Query: 220 GLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSS 279
            L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q            S  
Sbjct: 191 SLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQ------------SRV 250

Query: 280 SSSSSKIFILDVYDAFLSLIQGRGSGR------PCCFGVSSGFNCGSVDEKGNKKFVLCN 339
                  FI+D+++AF+++++ +GS R       CC G      CG   + G K + LC+
Sbjct: 251 MKKEQHFFIIDIHNAFMTVMKNKGSKRFKNPMKSCCEGY-----CGRSSD-GGKLYTLCD 309

Query: 340 DPKSAFFWDSVHPTQTGWSLAFS 354
           DPKS FFWD+VHPTQ GW   +S
Sbjct: 311 DPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of IVF0008195 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 227.3 bits (578), Expect = 2.8e-58
Identity = 137/326 (42.02%), Postives = 186/326 (57.06%), Query Frame = 0

Query: 40  RATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKS 99
           + TKLFVFGDSY DTGN       + +FP GITFPG P+GRFSDGRV TD+  K++GV++
Sbjct: 44  KPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRT 103

Query: 100 PIPFSIRNEVGEERLK-ESGINFAFGGTGVFDTSVPL-PNMTTQIDLFEQLRHAESGLSN 159
           PI +    + G  RL  + G+NFA+GG G F+T   L P  + QID FEQL    +  S 
Sbjct: 104 PITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLL-MRNVYSP 163

Query: 160 RDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTG 219
            D++ SVA  S+ GNDY  Y   NGS +G       VV Q+++D+KRIK LGV+K++V  
Sbjct: 164 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 223

Query: 220 LGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN-KQITNQQHSYSSSSSSS 279
             P  CLP    P   K C+ T ++    HN LL++ + KLN K+I N   S+ +     
Sbjct: 224 SPPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMT----- 283

Query: 280 SSSSSKIFILDVYDAFLSLIQGRG-SG--------RPCCFGVSSGFNCGSVDEKGNKKFV 339
                    LD+Y+AF+++ + +G SG        + CC     G  CG     G K + 
Sbjct: 284 ---------LDLYNAFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYT 343

Query: 340 LCNDPKSAFFWDSVHPTQTGWSLAFS 354
           LC+DPKS FFWD+VH +  GW   FS
Sbjct: 344 LCDDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of IVF0008195 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 1.1e-201
Identity = 359/370 (97.03%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP 60
           MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP
Sbjct: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP 60

Query: 61  FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN 120
           FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN
Sbjct: 61  FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN 120

Query: 121 FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT 180
           FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT
Sbjct: 121 FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT 180

Query: 181 NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 240
           NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI
Sbjct: 181 NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 240

Query: 241 NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG 300
           NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG
Sbjct: 241 NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG 300

Query: 301 SGR-----------PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 360
           SGR           PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS
Sbjct: 301 SGRVGLLKFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 360

BLAST of IVF0008195 vs. ExPASy TrEMBL
Match: A0A1S3CJ29 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 6.5e-191
Identity = 339/351 (96.58%), Postives = 340/351 (96.87%), Query Frame = 0

Query: 20  SGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 79
           +GQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG
Sbjct: 2   TGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 61

Query: 80  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 139
           RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT
Sbjct: 62  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 121

Query: 140 TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM 199
           TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM
Sbjct: 122 TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM 181

Query: 200 IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN 259
           IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN
Sbjct: 182 IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN 241

Query: 260 KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR-----------PCCFG 319
           KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR           PCCFG
Sbjct: 242 KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPCCFG 301

Query: 320 VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 360
           VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL
Sbjct: 302 VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 352

BLAST of IVF0008195 vs. ExPASy TrEMBL
Match: A0A5D3C686 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G001210 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.2e-190
Identity = 338/349 (96.85%), Postives = 338/349 (96.85%), Query Frame = 0

Query: 22  QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF 81
           QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF
Sbjct: 5   QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF 64

Query: 82  SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ 141
           SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ
Sbjct: 65  SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ 124

Query: 142 IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID 201
           IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID
Sbjct: 125 IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID 184

Query: 202 LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ 261
           LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ
Sbjct: 185 LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ 244

Query: 262 ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR-----------PCCFGVS 321
           ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR           PCCFGVS
Sbjct: 245 ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPCCFGVS 304

Query: 322 SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 360
           SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL
Sbjct: 305 SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of IVF0008195 vs. ExPASy TrEMBL
Match: A0A6J1IKX2 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478379 PE=3 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 6.0e-136
Identity = 243/363 (66.94%), Postives = 285/363 (78.51%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP 60
           MDF L+LLF+ F   +SLFSG+GSAGG Y++ HH+ HRLR TKLFVFGDSYVDTGNIL+P
Sbjct: 1   MDFVLRLLFSLFLL-ISLFSGRGSAGGVYNHHHHQRHRLRPTKLFVFGDSYVDTGNILIP 60

Query: 61  FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN 120
            SSAQ FPYGITFP KPSGRFSDGRVLTDFA +++G+KSPIPFSIRN VG  RLKE+G+N
Sbjct: 61  LSSAQSFPYGITFPRKPSGRFSDGRVLTDFAARYVGLKSPIPFSIRNRVGLRRLKETGVN 120

Query: 121 FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT 180
           FAFGGTGVFDTS P PNMTTQID F+QL+H  S  +  D   S ALVSVSGNDYS  + T
Sbjct: 121 FAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGHSLSTKTDNQFSAALVSVSGNDYSLDIIT 180

Query: 181 NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 240
           NG  QGLKPFIN+VVNQ+++DLKRI  LGVKKI VTGLGPLGCLP FTAP SFK+CN++I
Sbjct: 181 NGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNESI 240

Query: 241 NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQG-- 300
           NSFV+FHNFLLK+AV KLN++               +  SSK+F+LDVY AF+S+I+G  
Sbjct: 241 NSFVKFHNFLLKKAVKKLNQE---------------TKHSSKVFVLDVYGAFMSIIEGGK 300

Query: 301 -------RGSGRPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLA 355
                  +   +PCCFGV SGFNCGSVD +G K FVLC DPK AF+WD+VHPTQ GW+ A
Sbjct: 301 RYNNGEFKTPLKPCCFGVKSGFNCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAAA 347

BLAST of IVF0008195 vs. ExPASy TrEMBL
Match: A0A6J1EC94 (GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.7e-133
Identity = 240/364 (65.93%), Postives = 286/364 (78.57%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYS-NRHHRSHRLRATKLFVFGDSYVDTGNILV 60
           MDF L+LLF+     +SLFSG+ SAGG Y+ + HH+ HRLR TKLFVFGDSYVDTGNIL+
Sbjct: 1   MDFVLRLLFSLLLL-ISLFSGRVSAGGVYNHHHHHQRHRLRPTKLFVFGDSYVDTGNILI 60

Query: 61  PFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGI 120
           P SSAQQFPYGITFPGKPSGRFSDGRVLTDFA +++G+KSPIPF+++N VG  R KE+G+
Sbjct: 61  PLSSAQQFPYGITFPGKPSGRFSDGRVLTDFAARYVGLKSPIPFTVQNRVGLRRFKETGV 120

Query: 121 NFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLA 180
           NFAFGGTGVFDTS P PNMTTQID F+QL+H ES  +  D   S ALVSVSGNDYS  + 
Sbjct: 121 NFAFGGTGVFDTSFPFPNMTTQIDFFQQLQHGESLSTKTDNQFSAALVSVSGNDYSLDII 180

Query: 181 TNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQT 240
           TNG  QGLKPFIN+VVNQ+++DLKRI  LGVKKI VTGLGPLGCLP FTAP SFK+CN+T
Sbjct: 181 TNGPKQGLKPFINTVVNQIIVDLKRIGELGVKKIAVTGLGPLGCLPAFTAPSSFKKCNET 240

Query: 241 INSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR 300
           INSFV+FHNFLLK+AV KLN++               +  SSK+F+LD+Y AF+S+I+G 
Sbjct: 241 INSFVKFHNFLLKKAVKKLNQE---------------TKHSSKVFVLDMYGAFMSIIEGE 300

Query: 301 ---GSG------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSL 355
               +G      +PCCFGV SGF+CGSVD +G K FVLC DPK AF+WD+VHPTQ GW+ 
Sbjct: 301 KRYNNGVFKTPLKPCCFGVKSGFDCGSVDGEGKKMFVLCKDPKKAFYWDAVHPTQQGWAA 348

BLAST of IVF0008195 vs. NCBI nr
Match: KAA0025271.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 704 bits (1818), Expect = 1.58e-255
Identity = 359/370 (97.03%), Postives = 359/370 (97.03%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP 60
           MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP
Sbjct: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVP 60

Query: 61  FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN 120
           FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN
Sbjct: 61  FSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGIN 120

Query: 121 FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT 180
           FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT
Sbjct: 121 FAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLAT 180

Query: 181 NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 240
           NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI
Sbjct: 181 NGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTI 240

Query: 241 NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG 300
           NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG
Sbjct: 241 NSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRG 300

Query: 301 SGR-----------PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 359
           SGR           PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS
Sbjct: 301 SGRVGLLKFKTPLKPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 360

BLAST of IVF0008195 vs. NCBI nr
Match: XP_008462669.1 (PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo])

HSP 1 Score: 668 bits (1724), Expect = 1.71e-241
Identity = 339/351 (96.58%), Postives = 340/351 (96.87%), Query Frame = 0

Query: 20  SGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 79
           +GQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG
Sbjct: 2   TGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 61

Query: 80  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 139
           RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT
Sbjct: 62  RFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMT 121

Query: 140 TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM 199
           TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM
Sbjct: 122 TQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVM 181

Query: 200 IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN 259
           IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN
Sbjct: 182 IDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN 241

Query: 260 KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR-----------PCCFG 319
           KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR           PCCFG
Sbjct: 242 KQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPCCFG 301

Query: 320 VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 359
           VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL
Sbjct: 302 VSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 352

BLAST of IVF0008195 vs. NCBI nr
Match: TYK07397.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 665 bits (1717), Expect = 2.07e-240
Identity = 338/349 (96.85%), Postives = 338/349 (96.85%), Query Frame = 0

Query: 22  QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF 81
           QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF
Sbjct: 5   QGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRF 64

Query: 82  SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ 141
           SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ
Sbjct: 65  SDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTTQ 124

Query: 142 IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID 201
           IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID
Sbjct: 125 IDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMID 184

Query: 202 LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ 261
           LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ
Sbjct: 185 LKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQ 244

Query: 262 ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR-----------PCCFGVS 321
           ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGR           PCCFGVS
Sbjct: 245 ITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSGRVGLLKFKTPLKPCCFGVS 304

Query: 322 SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 359
           SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL
Sbjct: 305 SGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFSSFTSIL 353

BLAST of IVF0008195 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 625 bits (1613), Expect = 2.45e-224
Identity = 325/373 (87.13%), Postives = 338/373 (90.62%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSAGGYYSN-RH-HRSHRLRATKLFVFGDSYVDTGNIL 60
           MDFNLKLLFTFFFFT+SLFSGQGSAGG+Y N RH HRSH  R TKLFVFGDSYVDTGNIL
Sbjct: 1   MDFNLKLLFTFFFFTLSLFSGQGSAGGFYGNLRHRHRSHPSRGTKLFVFGDSYVDTGNIL 60

Query: 61  VPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESG 120
            PFSSA+QFPYGITFPGKPSGRFSDGRVLTDFA KHLGVKSPIPFSIR+EVGEERLKESG
Sbjct: 61  FPFSSAEQFPYGITFPGKPSGRFSDGRVLTDFAAKHLGVKSPIPFSIRSEVGEERLKESG 120

Query: 121 INFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGL-SNRDVHLSVALVSVSGNDYSFY 180
           INFAFGGTGVF+T VPLPNMTTQIDLFEQLR  ESGL SNRDVHLS+ALVSVSGNDYSFY
Sbjct: 121 INFAFGGTGVFNTLVPLPNMTTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFY 180

Query: 181 LATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240
           LATNGSAQGLKPFINSVVNQ+M+DL+RI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN
Sbjct: 181 LATNGSAQGLKPFINSVVNQIMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 240

Query: 241 QTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQ 300
           QTINSFVQFHNFLLKQAVDKLNKQIT QQH       SSSSSSSKIFILDVYDAFLS+IQ
Sbjct: 241 QTINSFVQFHNFLLKQAVDKLNKQITKQQHY------SSSSSSSKIFILDVYDAFLSIIQ 300

Query: 301 GRGSGR-----------PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQT 359
           GRGSGR           PCCFGVSS F CGSVDE+GNKKFVLCNDPKSAFFWDSVHPTQT
Sbjct: 301 GRGSGRVGLLKFKTPLKPCCFGVSSEFECGSVDEQGNKKFVLCNDPKSAFFWDSVHPTQT 360

BLAST of IVF0008195 vs. NCBI nr
Match: XP_038883594.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 573 bits (1476), Expect = 1.55e-203
Identity = 296/373 (79.36%), Postives = 316/373 (84.72%), Query Frame = 0

Query: 1   MDFNLKLLFTFFFFTVSLFSGQGSA--GGYYSNRHH---RSHRLRATKLFVFGDSYVDTG 60
           M+FNL LLF+FFFF +SLFSGQGSA  G YY + HH   R HRLR TKLFVFGDSYVDTG
Sbjct: 1   MNFNLSLLFSFFFFMISLFSGQGSASAGRYYGDHHHHHHRRHRLRPTKLFVFGDSYVDTG 60

Query: 61  NILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLK 120
           NIL+PFSSAQQFPYGITFPGKPSGRFSDGRVLTDF  KHLGVKSPIPFSIR+EVG ERLK
Sbjct: 61  NILLPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFLAKHLGVKSPIPFSIRSEVGVERLK 120

Query: 121 ESGINFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYS 180
           ESGINFAFGGTGVFDTSVPLPNMTTQID F+ LRH ES  SN D   SVALVSVSGNDYS
Sbjct: 121 ESGINFAFGGTGVFDTSVPLPNMTTQIDFFQHLRHVESVFSNGDAQHSVALVSVSGNDYS 180

Query: 181 FYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240
           FYLATNGSAQGLKPFINSVVNQ++IDLKRI+RLGVKKIVVTGLGPLGCLPIFTAPFSFKQ
Sbjct: 181 FYLATNGSAQGLKPFINSVVNQIVIDLKRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQ 240

Query: 241 CNQTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSL 300
           CNQTINSFV+FHNFLL QAV KLNK+  +            +SSSSKIF+LDVYDAFLS+
Sbjct: 241 CNQTINSFVRFHNFLLNQAVAKLNKETKH------------ASSSSKIFVLDVYDAFLSI 300

Query: 301 IQGRGSG-----------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPT 357
           IQ R              +PCCFGVSSGF CGSVDE+GNKKFVLCN+PKSAFFWDSVHPT
Sbjct: 301 IQSRSKKPGVVLEFKTPLKPCCFGVSSGFECGSVDEQGNKKFVLCNNPKSAFFWDSVHPT 360

BLAST of IVF0008195 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.0e-95
Identity = 183/364 (50.27%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 1   MDFNLKLLF-TFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILV 60
           MD  +KL F  F F   SL  G+ + G   SN++H  +  R TKLFVFGDSY DTGNI  
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEIN-GVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKK 60

Query: 61  PFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGI 120
            FSS+ +FPYGITFPGKP+GRFSDGRV TDF  K +G+KSPIP+  ++  G++RL + G+
Sbjct: 61  AFSSSWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRL-QYGM 120

Query: 121 NFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLA 180
           NFA+GGTGVF+T  PLPNMTTQID+F+ +          ++  SVALVSV+GNDYS ++A
Sbjct: 121 NFAYGGTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIA 180

Query: 181 TNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQT 240
            N  A     FI  VV+Q  ++L+RI  LGVKKI V  L PLGCLP FT   SF++CN+T
Sbjct: 181 LNRPASEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNET 240

Query: 241 INSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGR 300
            N+ V  HN LL+Q V KLN +                +  S   ILD+Y+AFL++ + +
Sbjct: 241 QNALVNLHNNLLQQVVAKLNNE----------------TKQSTFIILDLYNAFLTVFKNK 300

Query: 301 GSG----------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWS 354
           GS           +PCC GVS  +NCGSVDEKG KK+++C++PK+AFFWD +HPT+ GW 
Sbjct: 301 GSNPGSTRFESPLKPCCVGVSREYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWR 346

BLAST of IVF0008195 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 2.4e-84
Identity = 164/359 (45.68%), Postives = 226/359 (62.95%), Query Frame = 0

Query: 6   KLLFTFFFFTVSLFSGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQ 65
           KLL + F F+ S F   G+   Y   +H    RLR  +LFVFGDSY DTGNI    S + 
Sbjct: 5   KLLISLFLFSFSSFF-LGAESDY--PQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDSW 64

Query: 66  QFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGG 125
           + PYGITFP KPSGRFSDGRV TDF  ++LG+KSPIP++ ++  G+ERL   G+N+A+GG
Sbjct: 65  KIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLL-YGMNYAYGG 124

Query: 126 TGVFDT-SVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSA 185
           TGVF T   PLPNMTTQID F+++  A +  S  D+  S+ALVSV+GNDY+ +LA     
Sbjct: 125 TGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPL 184

Query: 186 QGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFV 245
             L  F+  VV+Q+ ++  RI +LGV KIV+  + PLGCLP  T   SF++CN T N+  
Sbjct: 185 TELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAST 244

Query: 246 QFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQGRGSG-- 305
             HN+LL +A+ +LN +                +  S   +LD Y+AFL++ + +G    
Sbjct: 245 NLHNYLLHKAIARLNNE----------------TKPSTFVVLDHYNAFLTVFKNKGPEPG 304

Query: 306 --------RPCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLAFS 354
                   +PCC GV+S ++C +VDEKG KK+++C DPK+AFFWD  HP++ GW   +S
Sbjct: 305 VSRFGNPLKPCCVGVNSSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYS 343

BLAST of IVF0008195 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 6.3e-61
Identity = 133/323 (41.18%), Postives = 188/323 (58.20%), Query Frame = 0

Query: 40  RATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKS 99
           +  KLFVFGDSY DTGN     + A   PYGITFPGKPSGR+ DG + TDF  K LG +S
Sbjct: 11  KVPKLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAES 70

Query: 100 PIPFSIRNEVGEERLKESGINFAFGGTGVFDTS--VPLPNMTTQIDLFEQLRHAESGLSN 159
           P  +      G ++  + G+NFAFGG+ + D+S   P PN+T Q++    L    +G   
Sbjct: 71  PYLYRTH---GRDKGLKRGMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDL--VLAGRVY 130

Query: 160 RDVHLS-VALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVT 219
            D+  S V+L+S +G DY +Y+  N  A GLK  +  VV+ + +++  +  L  KKI VT
Sbjct: 131 GDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVT 190

Query: 220 GLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSS 279
            L P+GCLP +T+  SFK CN++ ++ V+ HN LLK+ V KLN+Q            S  
Sbjct: 191 SLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQ------------SRV 250

Query: 280 SSSSSKIFILDVYDAFLSLIQGRGSGR------PCCFGVSSGFNCGSVDEKGNKKFVLCN 339
                  FI+D+++AF+++++ +GS R       CC G      CG   + G K + LC+
Sbjct: 251 MKKEQHFFIIDIHNAFMTVMKNKGSKRFKNPMKSCCEGY-----CGRSSD-GGKLYTLCD 309

Query: 340 DPKSAFFWDSVHPTQTGWSLAFS 354
           DPKS FFWD+VHPTQ GW   +S
Sbjct: 311 DPKSFFFWDAVHPTQEGWRSIYS 309

BLAST of IVF0008195 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 227.3 bits (578), Expect = 2.0e-59
Identity = 137/326 (42.02%), Postives = 186/326 (57.06%), Query Frame = 0

Query: 40  RATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKS 99
           + TKLFVFGDSY DTGN       + +FP GITFPG P+GRFSDGRV TD+  K++GV++
Sbjct: 44  KPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIGVRT 103

Query: 100 PIPFSIRNEVGEERLK-ESGINFAFGGTGVFDTSVPL-PNMTTQIDLFEQLRHAESGLSN 159
           PI +    + G  RL  + G+NFA+GG G F+T   L P  + QID FEQL    +  S 
Sbjct: 104 PITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLL-MRNVYSP 163

Query: 160 RDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTG 219
            D++ SVA  S+ GNDY  Y   NGS +G       VV Q+++D+KRIK LGV+K++V  
Sbjct: 164 ADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLVAL 223

Query: 220 LGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAVDKLN-KQITNQQHSYSSSSSSS 279
             P  CLP    P   K C+ T ++    HN LL++ + KLN K+I N   S+ +     
Sbjct: 224 SPPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMT----- 283

Query: 280 SSSSSKIFILDVYDAFLSLIQGRG-SG--------RPCCFGVSSGFNCGSVDEKGNKKFV 339
                    LD+Y+AF+++ + +G SG        + CC     G  CG     G K + 
Sbjct: 284 ---------LDLYNAFVTIFKNKGVSGVSTFPDPFKACC-ATKRGTFCGDRSLSGKKLYT 343

Query: 340 LCNDPKSAFFWDSVHPTQTGWSLAFS 354
           LC+DPKS FFWD+VH +  GW   FS
Sbjct: 344 LCDDPKSFFFWDNVHISDQGWRSVFS 348

BLAST of IVF0008195 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 90/302 (29.80%), Postives = 132/302 (43.71%), Query Frame = 0

Query: 60  PFSSAQQFPYGITFPGKPSGRFSDGRVLTDFAGKHLGVKSPIPFSIRNEVGEERLKESGI 119
           P + + + PYGITFPGKPSGR+SDG   TDF G                           
Sbjct: 3   PTALSWKLPYGITFPGKPSGRYSDGLTATDFLGSE------------------------- 62

Query: 120 NFAFGGTGVFDTSVP-LPNMTTQIDLFEQLRHAESGLS-NRDVHLSVALVSVSGNDYSFY 179
                   VFD+ V   PN++TQ+     L  A    + + D+  S AL+S SG DY  +
Sbjct: 63  --------VFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGF 122

Query: 180 LATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCN 239
           +  N +      F+  +V  +                                     CN
Sbjct: 123 IDQNPNMAAYPAFVEFIVEDI------------------------------------HCN 182

Query: 240 QTINSFVQFHNFLLKQAVDKLNKQITNQQHSYSSSSSSSSSSSSKIFILDVYDAFLSLIQ 299
           ++ +  V+ HN  LK+AV KLNK+                +   +  I+D++ AF+++++
Sbjct: 183 ESYSDLVRLHNESLKKAVAKLNKE------------DKFRTKGDRFVIVDLHKAFMTILE 218

Query: 300 GRGSGR------PCCFGVSSGFNCGSVDEKGNKKFVLCNDPKSAFFWDSVHPTQTGWSLA 354
            +G+ R      PCC G     +C  +D KG KK+ LCNDPKSAFFWD ++PTQ GW   
Sbjct: 243 KKGNKRFKSPLKPCCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSI 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LZS74.2e-9450.27GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2... [more]
Q9SF943.3e-8345.68GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2... [more]
Q9LZS92.1e-6140.57GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3... [more]
Q9LZS88.8e-6041.18GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3... [more]
Q8RWJ42.8e-5842.02GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2... [more]
Match NameE-valueIdentityDescription
A0A5A7SLZ41.1e-20197.03GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541... [more]
A0A1S3CJ296.5e-19196.58GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3... [more]
A0A5D3C6864.2e-19096.85GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202... [more]
A0A6J1IKX26.0e-13666.94GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111478379 ... [more]
A0A6J1EC943.7e-13365.93GDSL esterase/lipase At5g03610-like isoform X3 OS=Cucurbita moschata OX=3662 GN=... [more]
Match NameE-valueIdentityDescription
KAA0025271.11.58e-25597.03GDSL esterase/lipase [Cucumis melo var. makuwa][more]
XP_008462669.11.71e-24196.58PREDICTED: GDSL esterase/lipase At5g03610-like, partial [Cucumis melo][more]
TYK07397.12.07e-24096.85GDSL esterase/lipase [Cucumis melo var. makuwa][more]
XP_004143379.22.45e-22487.13GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protei... [more]
XP_038883594.11.55e-20379.36GDSL esterase/lipase At5g03610-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G03610.13.0e-9550.27GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT3G09930.12.4e-8445.68GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03600.16.3e-6141.18SGNH hydrolase-type esterase superfamily protein [more]
AT2G36325.12.0e-5942.02GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT5G03590.11.2e-3029.80GDSL-like Lipase/Acylhydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 29..359
e-value: 1.2E-54
score: 187.8
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 44..352
e-value: 4.6E-26
score: 92.7
NoneNo IPR availablePANTHERPTHR46020OSJNBB0059K02.9 PROTEINcoord: 17..356
NoneNo IPR availablePANTHERPTHR46020:SF4OSJNBB0059K02.9 PROTEINcoord: 17..356
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 43..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008195.1IVF0008195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016788 hydrolase activity, acting on ester bonds