IVF0008035 (gene) Melon (IVF77) v1

Overview
NameIVF0008035
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionExopolygalacturonase clone
Locationchr01: 29165962 .. 29168837 (-)
RNA-Seq ExpressionIVF0008035
SyntenyIVF0008035
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAGAACAAAAGAGCTCATACTCATAACAAAGCACAATCTGGGGGGGTTAAGAAGTCACCATCACCCATGGCCATTACAGAGAGATTGCACATCGCTGCCGCCCGTGGTTTAATATTAGGGTTGGCATTGCTCTCTTCGATGGCCCGGTGCAGCTCTGCGATTGCATCTGATTATTCCGGTGACTTTGTTGGCATTCGAAGCCGCTTCCTCATTGGAGCAGCGCCCGCCACCACCACCCCCACTGCAAAGGTTTTCGATATTATAGCCCATGGTGCCAAAGCTGATGGCAGGACGGATAGTACACAAGTGAGTGATGGTTTAGATCGCATATATTATTTGGTGATCGTTGGAATGCCTTAAATTGCACAAGTTTCACATGTACTGTATTTACGATTTCTGGGATTAGGGTTTAGAGAGTTGTTATATATATATATATATTTTGTTGCATCTCACCCTAATAACAAACTAATCTCACCATCTACCCATTCAATAATATAATGTTAGCTACCACACAATTTTTTTTTCTTGTTTTCTTTATTTGTCGACTTTGTAATGAGCATGGAGGTTAAATATTTGAGATTTGGAAATGTGCAGGCATTCATGCAGACGTGGGTTAAAGCATGTCACTCGGGTGGCCCGGCCAAGGTGGTATTCCCACCGGGGACGTTTTTGACAGGGCCATTGGTGTATGCTGGACCGTGTGATGGGCCAATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCCCCAGAGTGGATTCTATTTGAATCTGTCACTAAGTTGAATCTCATTGGTAAGGGAACCTTTGATGGACAAGGTGCAGAGGCATGGAAATATAATGATTGTAGCAAACACCCTCAATGCACTCTTCCCCCAACCGTAAGCATTCATTCAATTGTCTTTTAATTTTGTGTCTAATAGATTGAGAAGCTTTAAATCATATCTTCATTTTAGGCATGATCCACGTTAATTATTGACAATATCAAAGTTACCGATTGGGTGCTAGCTCTAAAATTAAAAGACTAAAATTGACAATTTAAGACCCTTATTTGTTTGTATATTCACACTCTTCATAATATATACAAATAGGCTACTCCTAATCTTTCATTAGATATTGATGTTATCTAATACTATCTGATAACTATTACTGAAAAGCAAACTAAAGATTTGAACATGCTAGAAAATAATTTTTTTTCCTTAACATTGTTACATTTTTGTTTTATAACATAGATATGAATTCAAATGTTTCAAAGCATAGTTTTGGGTTCTAGGATTTAAACATTTTTTTTTTAACAAAATACAAAAGGAAGATGAGGTAAGTAGGTGCACCAGAGTTGAACCAAGATTGATGGTTCTAGGGTTTCAGACACTTTTGCATTATAAACACAACATTATTTTGAATGATCAAGTAAATGAATTTAATAAATGTATGGAAGAAAGGTTTTTGTCAATTATTTTAGGGTTTTCTGTAATAAATTTGGATGCAGTCAATAAAGTTCAACAAGGTAACCCATGGGTCGATGGAGGGTTTTACTTCAGTGAACAGCAAAGCGTTCCACATATTCGTGGTCTTAAGCCACAATATAAAAATCAACAATATACACATAGTTGCTCCTGGTAATAGCCCAAACACTGATGGTGTTCATATAAGCCAGACTGATGTAGTTAACGTTACAAATAGCATCATTGGAACCGGAGATGATTGTGTTTCAATTGGCCATGGATCCACCAATATCAATGTTCTCAACATCACTTGTGGCCCTGGACATGGCATCAGGTATATACCAATGTTAAGAGAATCCCCGATTAGAAAGCTCAGAGAAAGCTAGTCACTTCTATAAATTGCATAGAGTTGACTCTTCCATTATGGATTAATTTTAAGATGAAGTCTGATGTTTAACTAATACATATTCAAACAATCTAATTTAATTCATTAGAAATATGAGTTTTAAGAGTTGAGATCAGACCATTTGGATTGACTTTAAAGCATTTGGTCCAAATCCGTCCTAAAATTTAGTTTACAAGCTTTGTGATTTGTAGGCTTGAGGTTTTATTGAAAACAGTTTTGTAACCAAAACAATTGATAACAAAAGTAGCAATTCATTTTATCTCATAAAACATCAATTGCGAAATCTCATTTTTTGTTTTATAACAGAGTTAAATGGATAGACCAAATTTATGTATTGAAATTTTGCAGTGTGGGAAGCCTTGGGAAATATAAAGATGAGAAGGAAGTACGTGGAATTTTTGTGTCAAATTGCACAATAAGAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACTAGAATCACCTTCCAAGACATTGTCTTAGATAAGGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCAAAAAAGAAGAGAAGTGTAAGTAACTAATTAGACTCTGTGTTCGATAACAATTACAAACATGCTTAATTACGTAATCAACATTTAACATTGTGTCATGATTGTCATGCAGCCATCTCAGGTGAAGATCAGCGACGTCCAGTTCAAGAACATTCGAGGCACCACGATATCTCCCGTTGCCGTTTCGTTGCAGTGCAGCGCTGCCTTGCCGTGCGACAGTATTAAATTGGAACAGATTGATGTGGCCTTTTCTGGCTCTCGTTCAAAGCAACCATTTGGAAATTCATGTTTGAATGCCAAGATTACAACCATAGGAAAACAGAACCCACCGGCTTGTGTTTAGCTTTATGACAATGTATAGCTCAATTTATATAATCATTCCATATTCATTTATAACTAATTGGTTGACTAAGTATTATAATAGTGAGGGTTTCGTTACGAGTCTTTCGAC

mRNA sequence

GTTAGAACAAAAGAGCTCATACTCATAACAAAGCACAATCTGGGGGGGTTAAGAAGTCACCATCACCCATGGCCATTACAGAGAGATTGCACATCGCTGCCGCCCGTGGTTTAATATTAGGGTTGGCATTGCTCTCTTCGATGGCCCGGTGCAGCTCTGCGATTGCATCTGATTATTCCGGTGACTTTGTTGGCATTCGAAGCCGCTTCCTCATTGGAGCAGCGCCCGCCACCACCACCCCCACTGCAAAGGTTTTCGATATTATAGCCCATGGTGCCAAAGCTGATGGCAGGACGGATAGTACACAAGCATTCATGCAGACGTGGGTTAAAGCATGTCACTCGGGTGGCCCGGCCAAGGTGGTATTCCCACCGGGGACGTTTTTGACAGGGCCATTGGTGTATGCTGGACCGTGTGATGGGCCAATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCCCCAGAGTGGATTCTATTTGAATCTGTCACTAAGTTGAATCTCATTGGTAAGGGAACCTTTGATGGACAAGGTGCAGAGGCATGGAAATATAATGATTGTAGCAAACACCCTCAATGCACTCTTCCCCCAACCTCAATAAAGTTCAACAAGGTAACCCATGGGTCGATGGAGGGTTTTACTTCAGTGAACAGCAAAGCGTTCCACATATTCGTGGTCTTAAGCCACAATATAAAAATCAACAATATACACATAGTTGCTCCTGGTAATAGCCCAAACACTGATGGTGTTCATATAAGCCAGACTGATGTAGTTAACGTTACAAATAGCATCATTGGAACCGGAGATGATTGTGTTTCAATTGGCCATGGATCCACCAATATCAATGTTCTCAACATCACTTGTGGCCCTGGACATGGCATCAGTGTGGGAAGCCTTGGGAAATATAAAGATGAGAAGGAAGTACGTGGAATTTTTGTGTCAAATTGCACAATAAGAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACTAGAATCACCTTCCAAGACATTGTCTTAGATAAGGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCAAAAAAGAAGAGAAGTCCATCTCAGGTGAAGATCAGCGACGTCCAGTTCAAGAACATTCGAGGCACCACGATATCTCCCGTTGCCGTTTCGTTGCAGTGCAGCGCTGCCTTGCCGTGCGACAGTATTAAATTGGAACAGATTGATGTGGCCTTTTCTGGCTCTCGTTCAAAGCAACCATTTGGAAATTCATGTTTGAATGCCAAGATTACAACCATAGGAAAACAGAACCCACCGGCTTGTGTTTAGCTTTATGACAATGTATAGCTCAATTTATATAATCATTCCATATTCATTTATAACTAATTGGTTGACTAAGTATTATAATAGTGAGGGTTTCGTTACGAGTCTTTCGAC

Coding sequence (CDS)

ATGGCCATTACAGAGAGATTGCACATCGCTGCCGCCCGTGGTTTAATATTAGGGTTGGCATTGCTCTCTTCGATGGCCCGGTGCAGCTCTGCGATTGCATCTGATTATTCCGGTGACTTTGTTGGCATTCGAAGCCGCTTCCTCATTGGAGCAGCGCCCGCCACCACCACCCCCACTGCAAAGGTTTTCGATATTATAGCCCATGGTGCCAAAGCTGATGGCAGGACGGATAGTACACAAGCATTCATGCAGACGTGGGTTAAAGCATGTCACTCGGGTGGCCCGGCCAAGGTGGTATTCCCACCGGGGACGTTTTTGACAGGGCCATTGGTGTATGCTGGACCGTGTGATGGGCCAATGACGGTTGAAATTCAAGGGACTGTGAAGGCAACAACTGATATTACTGAATATTCATCCCCAGAGTGGATTCTATTTGAATCTGTCACTAAGTTGAATCTCATTGGTAAGGGAACCTTTGATGGACAAGGTGCAGAGGCATGGAAATATAATGATTGTAGCAAACACCCTCAATGCACTCTTCCCCCAACCTCAATAAAGTTCAACAAGGTAACCCATGGGTCGATGGAGGGTTTTACTTCAGTGAACAGCAAAGCGTTCCACATATTCGTGGTCTTAAGCCACAATATAAAAATCAACAATATACACATAGTTGCTCCTGGTAATAGCCCAAACACTGATGGTGTTCATATAAGCCAGACTGATGTAGTTAACGTTACAAATAGCATCATTGGAACCGGAGATGATTGTGTTTCAATTGGCCATGGATCCACCAATATCAATGTTCTCAACATCACTTGTGGCCCTGGACATGGCATCAGTGTGGGAAGCCTTGGGAAATATAAAGATGAGAAGGAAGTACGTGGAATTTTTGTGTCAAATTGCACAATAAGAAACACCACAAATGGAGTAAGGATCAAGACATGGGCTGCTTCACCTCCTGGCCAAGCTACTAGAATCACCTTCCAAGACATTGTCTTAGATAAGGTCCGAAACCCTATCATCATCGATCAAACTTACGGCTCAAAAAAGAAGAGAAGTCCATCTCAGGTGAAGATCAGCGACGTCCAGTTCAAGAACATTCGAGGCACCACGATATCTCCCGTTGCCGTTTCGTTGCAGTGCAGCGCTGCCTTGCCGTGCGACAGTATTAAATTGGAACAGATTGATGTGGCCTTTTCTGGCTCTCGTTCAAAGCAACCATTTGGAAATTCATGTTTGAATGCCAAGATTACAACCATAGGAAAACAGAACCCACCGGCTTGTGTTTAG

Protein sequence

MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTIGKQNPPACV
Homology
BLAST of IVF0008035 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 7.7e-103
Identity = 195/427 (45.67%), Postives = 273/427 (63.93%), Query Frame = 0

Query: 9   IAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTAKVFDIIAH 68
           +A A  + +G  +L ++    S +A   +G+++G R     G A  +  PT  V+DI   
Sbjct: 8   VAKANNINVGSLILMALV-FGSCVA---NGEYLGGRR----GLAANSGNPT--VYDITKF 67

Query: 69  GAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTV 128
           GA  DG T++ +AF+ TW++ C S  PA ++ P GTFL GP+++AGPC   +TV + GT+
Sbjct: 68  GAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVTVNVIGTI 127

Query: 129 KATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFN 188
            ATT  + Y++PEW LFE V  L L G GTF G+G   WK + C K  QC LPPTS+KF 
Sbjct: 128 IATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQCNLPPTSLKFR 187

Query: 189 KVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNS 248
            + +  + G +SVN+KAFH+F+V + N+ I NI + AP  SPNTDG+H+S  D V++ +S
Sbjct: 188 NMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDS 247

Query: 249 IIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTN 308
            I TGDDCVS+G GS N+ V  + CGPGHG+SVGSLGKYK+E++V GI V+NCT+  T N
Sbjct: 248 TIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDN 307

Query: 309 GVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIR 368
           G+RIKTW  S P +A  I F++I++  V+NPIIIDQ YGS  +   SQV ISD+ FKNIR
Sbjct: 308 GLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGS--RGGDSQVAISDILFKNIR 367

Query: 369 GTTISPVAVSLQCSAALPCDSIKLEQIDVAFSG-------SRSKQPFGNSCLNAKITTIG 428
           GTTI+   V + CS ++PC  + +  +++ + G       S S    G  C NA +   G
Sbjct: 368 GTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVGALCDNANVIFGG 420

BLAST of IVF0008035 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.4e-91
Identity = 166/370 (44.86%), Postives = 236/370 (63.78%), Query Frame = 0

Query: 63  FDIIA-HGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMT 122
           FD++A  GAKADG+TD ++ F+  W +AC S  P+ VV P GT+L   +   GPC  P+ 
Sbjct: 29  FDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIE 88

Query: 123 VEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLP 182
           + +QGT++A  D + +  P W+ F SV    + G G FDGQG+ A++ N C      +  
Sbjct: 89  INVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKL 148

Query: 183 PTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTD 242
           P +I+F+ VT+  ++  TS +SK FHI V    NI +  + I AP  SPNTDG+H+ +++
Sbjct: 149 PVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSE 208

Query: 243 VVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNC 302
            VN+  S I TGDDC+SIG G+ N+ +  ITCGPGHGIS+GSLGK+++E+ V GI +SNC
Sbjct: 209 GVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNC 268

Query: 303 TIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTY---GSKKKRSPSQVK 362
           TI NT+NG RIKTW     G  + I F+DI ++ V +PI+IDQ Y      KK   S+VK
Sbjct: 269 TITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVK 328

Query: 363 ISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKIT 422
           +S++ FKNIRGT+  P A+   CS + PC +++L  ID+  +G+   +P  + CLN K  
Sbjct: 329 LSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIQHNGA---EPATSQCLNVKPI 388

Query: 423 TIGKQNPPAC 429
           TIGK NP  C
Sbjct: 389 TIGKLNPIPC 395

BLAST of IVF0008035 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.9e-91
Identity = 162/371 (43.67%), Postives = 236/371 (63.61%), Query Frame = 0

Query: 59  TAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDG 118
           +  VF++  +GAK  G  D +QA M+ W  AC S GP+ V+ P G +  G +   GPC G
Sbjct: 6   SGSVFNVNDYGAKGAG--DISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCKG 65

Query: 119 -PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQ 178
             +  +I G VKA  D +++ S  W+ F  +  L + G GT DGQG  AW  N+C K+P 
Sbjct: 66  SKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVSGTGTLDGQGQTAWAKNNCDKNPN 125

Query: 179 CTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHI 238
           C     +++F+ + H  +   TS+NSK FHI V+   +I   ++ + APG S NTDG+H+
Sbjct: 126 CKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINTDGIHV 185

Query: 239 SQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIF 298
             +  V +TN+ I TGDDC+SIG GS N+ +  + CGPGHGIS+GSLG+Y +EKEVRGI 
Sbjct: 186 GISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKEVRGIT 245

Query: 299 VSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQ---TYGSKKKRSP 358
           V  CT   T NGVR+KTW  SPPG AT +TFQD+ ++ V+NP+I+DQ    YG   +++P
Sbjct: 246 VKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQCSRQAP 305

Query: 359 SQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLN 418
           S++K+S++ F NIRGT+   VAV + CS  +PC ++K+ +I++++ G+    P  ++C N
Sbjct: 306 SRIKLSNINFNNIRGTSTGKVAVVIACSHGMPCSNMKIGEINLSYRGAGG--PATSTCSN 365

Query: 419 AKITTIGKQNP 426
            K T  GKQ P
Sbjct: 366 VKPTFSGKQVP 372

BLAST of IVF0008035 vs. ExPASy Swiss-Prot
Match: Q39786 (Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.5e-90
Identity = 164/370 (44.32%), Postives = 235/370 (63.51%), Query Frame = 0

Query: 63  FDIIA-HGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMT 122
           FD++A  GAKADG+TD ++ F+  W +AC S  P+ VV P GT+L   +   GPC  P+ 
Sbjct: 29  FDVVAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPCKAPIE 88

Query: 123 VEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLP 182
           + +QGT++A  D + +  P W+ F SV    + G G FDGQG+ A++ N C      +  
Sbjct: 89  INVQGTIQAPADPSAFKDPNWVRFYSVENFKMFGGGIFDGQGSIAYEKNTCENREFRSKL 148

Query: 183 PTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTD 242
           P +I+F+ +T+  ++  TS +SK FHI V    NI +  + I AP  SPNTDG+H+ +++
Sbjct: 149 PVNIRFDFLTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMGKSE 208

Query: 243 VVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNC 302
            VN+  S I TGDDC+SIG G+ N+ +  ITCGPGHGIS+GSLGK+++E+ V GI +SNC
Sbjct: 209 GVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKISNC 268

Query: 303 TIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTY---GSKKKRSPSQVK 362
           TI NT+NG RIKTW     G  + I F+DI ++ V +PI+IDQ Y      KK   S+VK
Sbjct: 269 TITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNEESKVK 328

Query: 363 ISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKIT 422
           +S++ FKNIRGT+  P A+   CS + PC +++L  ID+  +G+   +P  + CLN K  
Sbjct: 329 LSNISFKNIRGTSALPEAIKFICSGSSPCQNVELADIDIKHNGA---EPATSQCLNVKPI 388

Query: 423 TIGKQNPPAC 429
           T GK NP  C
Sbjct: 389 TSGKLNPIPC 395

BLAST of IVF0008035 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 325.9 bits (834), Expect = 7.0e-88
Identity = 173/385 (44.94%), Postives = 237/385 (61.56%), Query Frame = 0

Query: 49  IGAAPATTTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTG 108
           +GA  +   P A V D+ A GAK D +TD + AF   W +AC +G  + +  P G ++  
Sbjct: 57  VGAKVSGAKPGAAV-DVKASGAKGDSKTDDSAAFAAAWKEACAAG--STITVPKGEYMVE 116

Query: 109 PLVYAGPCDGPMTVEIQGTVKATTDI-TEYSSPEWILFESVTKLNLIG-KGTFDGQGAEA 168
            L + GPC GP+T+E+ G  KA   + T      WI FE++    L G K  FDGQG+ A
Sbjct: 117 SLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLA 176

Query: 169 WKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAP 228
           WK NDC+K  +C   P +I+F  +T+  +   TS NSK FH+ ++   NI +++I I AP
Sbjct: 177 WKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAP 236

Query: 229 GNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGK 288
             S NTDG+HI +++ VN+  + I TGDDCVSIG G+ N+ V N+ CGPGHGIS+GSLG+
Sbjct: 237 PESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGR 296

Query: 289 YKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQ-- 348
           Y +E+ V+G+ V  C I+NT NGVRIKTW  SPPG A+ I F+DI +D V  P++IDQ  
Sbjct: 297 YPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEY 356

Query: 349 -TYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSR 408
             YG  K   PSQVK+SDV  K I+GT+ + VAV L CS  +PC +I L  I++  +G  
Sbjct: 357 CPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKE 416

Query: 409 SKQPFGNSCLNAKITTIGKQNPPAC 429
              P  ++C N K    GK  P AC
Sbjct: 417 G--PAVSACSNIKPILSGKLVPAAC 436

BLAST of IVF0008035 vs. ExPASy TrEMBL
Match: A0A5D3D1R6 (Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001270 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 5.9e-247
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60
           MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA
Sbjct: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60

Query: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120
           KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM
Sbjct: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120

Query: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180
           TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL
Sbjct: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180

Query: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240
           PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT
Sbjct: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240

Query: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300
           DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN
Sbjct: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300

Query: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360
           CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS
Sbjct: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360

Query: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420
           DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI
Sbjct: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420

Query: 421 GKQNPPACV 430
           GKQNPPACV
Sbjct: 421 GKQNPPACV 429

BLAST of IVF0008035 vs. ExPASy TrEMBL
Match: A0A1S3BRS3 (exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 SV=1)

HSP 1 Score: 862.8 bits (2228), Expect = 5.9e-247
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60
           MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA
Sbjct: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60

Query: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120
           KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM
Sbjct: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120

Query: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180
           TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL
Sbjct: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180

Query: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240
           PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT
Sbjct: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240

Query: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300
           DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN
Sbjct: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300

Query: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360
           CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS
Sbjct: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360

Query: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420
           DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI
Sbjct: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420

Query: 421 GKQNPPACV 430
           GKQNPPACV
Sbjct: 421 GKQNPPACV 429

BLAST of IVF0008035 vs. ExPASy TrEMBL
Match: A0A5A7TXI9 (Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold427G00200 PE=3 SV=1)

HSP 1 Score: 861.3 bits (2224), Expect = 1.7e-246
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60
           MAITERLHIAAARGLILGLALLSSMA+CSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA
Sbjct: 1   MAITERLHIAAARGLILGLALLSSMAQCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60

Query: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120
           KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM
Sbjct: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120

Query: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180
           TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL
Sbjct: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180

Query: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240
           PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT
Sbjct: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240

Query: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300
           DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN
Sbjct: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300

Query: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360
           CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS
Sbjct: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360

Query: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420
           DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI
Sbjct: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420

Query: 421 GKQNPPACV 430
           GKQNPPACV
Sbjct: 421 GKQNPPACV 429

BLAST of IVF0008035 vs. ExPASy TrEMBL
Match: A0A0A0K9C4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.9e-223
Identity = 387/433 (89.38%), Postives = 401/433 (92.61%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPA----TT 60
           M I ER H+AA RGL+LGL LLSSM  CSSA A  YSGDF G RSRFL GAAPA    TT
Sbjct: 1   MTIPERFHVAAVRGLVLGLTLLSSMVWCSSAAAPHYSGDFFGTRSRFLTGAAPATTTTTT 60

Query: 61  TPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPC 120
           TPTAKVF+IIAHGAKADGRTDSTQAFMQ WVKACHS GPAKVVFPPGTFLTGPLVYAGPC
Sbjct: 61  TPTAKVFNIIAHGAKADGRTDSTQAFMQAWVKACHSSGPAKVVFPPGTFLTGPLVYAGPC 120

Query: 121 DGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHP 180
           DGPMTVEIQGTVKATTDI++YSS EWILFESVT LNLIG+GTFDGQGA+ WKYNDCSKHP
Sbjct: 121 DGPMTVEIQGTVKATTDISQYSSAEWILFESVTGLNLIGRGTFDGQGADTWKYNDCSKHP 180

Query: 181 QCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVH 240
           QC +PPTSIKFNKVT G MEG TSVNSKAFHIFVVLSHNIKINN+HIVAPGNSPNTDGVH
Sbjct: 181 QCIIPPTSIKFNKVTQGLMEGITSVNSKAFHIFVVLSHNIKINNVHIVAPGNSPNTDGVH 240

Query: 241 ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGI 300
           ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKY+DEKEVRGI
Sbjct: 241 ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYRDEKEVRGI 300

Query: 301 FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQ 360
           FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQ+IVLDKVRNPIIIDQ YGSK K+SPSQ
Sbjct: 301 FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLDKVRNPIIIDQNYGSKTKKSPSQ 360

Query: 361 VKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAK 420
           VK+SDVQFKNIRGTTISPVAVSLQCSAALPCD IKLEQIDVAFSG+  KQPF NSCLNAK
Sbjct: 361 VKVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQIDVAFSGTHLKQPFANSCLNAK 420

Query: 421 ITTIGKQNPPACV 430
           ITTIGKQNPPACV
Sbjct: 421 ITTIGKQNPPACV 433

BLAST of IVF0008035 vs. ExPASy TrEMBL
Match: A0A6J1GQF2 (exopolygalacturonase clone GBGE184 OS=Cucurbita moschata OX=3662 GN=LOC111456159 PE=3 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 3.4e-178
Identity = 314/437 (71.85%), Postives = 357/437 (81.69%), Query Frame = 0

Query: 1   MAITERLHIA----AARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPAT- 60
           MAI  R HIA    A RG++LGLA LS +ARCSS  A  +SGDF+ +R RFL   AP+T 
Sbjct: 1   MAIAGRFHIAVAASAVRGVVLGLAWLSCVARCSSTPAPHFSGDFLPLRGRFLANTAPSTP 60

Query: 61  ---TTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVY 120
              TTP  KVFDI  +GAK+DG+  +TQAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVY
Sbjct: 61  STPTTPGTKVFDITTYGAKSDGKKINTQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVY 120

Query: 121 AGPCDGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDC 180
           AGPCD PMTVEIQGTV ATTDITEYSSP WILFES+T LNLIG GTFDGQG E W YNDC
Sbjct: 121 AGPCDAPMTVEIQGTVLATTDITEYSSPVWILFESITGLNLIGNGTFDGQGQETWMYNDC 180

Query: 181 SKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNT 240
             +P C +PPTS+KFNKVT+G MEG +S+NSK FHIF+VLSH++++NN++I+AP  SPNT
Sbjct: 181 RDNPSCIMPPTSLKFNKVTNGLMEGISSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNT 240

Query: 241 DGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKE 300
           DG+HISQTD V +T+SIIGTGDDCVSIGHGS N+ V NI CGPGHGISVGSLGKYKDEK+
Sbjct: 241 DGIHISQTDNVTITSSIIGTGDDCVSIGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKD 300

Query: 301 VRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKR 360
           V GIFVSNCT++NTT GVRIKTWA SPP QAT I FQDIVLD V++PIIIDQ YGSKK +
Sbjct: 301 VSGIFVSNCTLKNTTFGVRIKTWADSPPSQATGIHFQDIVLDSVKSPIIIDQFYGSKKNK 360

Query: 361 SPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNS 420
            PS+VKISDV +KNIRGTTIS V VSLQCS+A+PCD I LE ID+AF GS R K   GNS
Sbjct: 361 KPSKVKISDVSYKNIRGTTISVVPVSLQCSSAVPCDGITLEDIDLAFIGSDRKKALVGNS 420

Query: 421 CLNAKITTIGKQNPPAC 429
           CLNAK    GKQNPPAC
Sbjct: 421 CLNAKFKATGKQNPPAC 437

BLAST of IVF0008035 vs. NCBI nr
Match: XP_008451547.1 (PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopolygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 863 bits (2230), Expect = 0.0
Identity = 429/429 (100.00%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60
           MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA
Sbjct: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60

Query: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120
           KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM
Sbjct: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120

Query: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180
           TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL
Sbjct: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180

Query: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240
           PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT
Sbjct: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240

Query: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300
           DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN
Sbjct: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300

Query: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360
           CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS
Sbjct: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360

Query: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420
           DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI
Sbjct: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420

Query: 421 GKQNPPACV 429
           GKQNPPACV
Sbjct: 421 GKQNPPACV 429

BLAST of IVF0008035 vs. NCBI nr
Match: KAA0046656.1 (exopolygalacturonase clone [Cucumis melo var. makuwa])

HSP 1 Score: 862 bits (2226), Expect = 1.79e-315
Identity = 428/429 (99.77%), Postives = 429/429 (100.00%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60
           MAITERLHIAAARGLILGLALLSSMA+CSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA
Sbjct: 1   MAITERLHIAAARGLILGLALLSSMAQCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTA 60

Query: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120
           KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM
Sbjct: 61  KVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPM 120

Query: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180
           TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL
Sbjct: 121 TVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTL 180

Query: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240
           PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT
Sbjct: 181 PPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQT 240

Query: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300
           DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN
Sbjct: 241 DVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSN 300

Query: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360
           CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS
Sbjct: 301 CTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKIS 360

Query: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420
           DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI
Sbjct: 361 DVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAKITTI 420

Query: 421 GKQNPPACV 429
           GKQNPPACV
Sbjct: 421 GKQNPPACV 429

BLAST of IVF0008035 vs. NCBI nr
Match: XP_011659369.1 (exopolygalacturonase clone GBGE184 [Cucumis sativus] >KGN44972.1 hypothetical protein Csa_015742 [Cucumis sativus])

HSP 1 Score: 783 bits (2023), Expect = 1.62e-284
Identity = 387/433 (89.38%), Postives = 401/433 (92.61%), Query Frame = 0

Query: 1   MAITERLHIAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTT--- 60
           M I ER H+AA RGL+LGL LLSSM  CSSA A  YSGDF G RSRFL GAAPATTT   
Sbjct: 1   MTIPERFHVAAVRGLVLGLTLLSSMVWCSSAAAPHYSGDFFGTRSRFLTGAAPATTTTTT 60

Query: 61  -PTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPC 120
            PTAKVF+IIAHGAKADGRTDSTQAFMQ WVKACHS GPAKVVFPPGTFLTGPLVYAGPC
Sbjct: 61  TPTAKVFNIIAHGAKADGRTDSTQAFMQAWVKACHSSGPAKVVFPPGTFLTGPLVYAGPC 120

Query: 121 DGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHP 180
           DGPMTVEIQGTVKATTDI++YSS EWILFESVT LNLIG+GTFDGQGA+ WKYNDCSKHP
Sbjct: 121 DGPMTVEIQGTVKATTDISQYSSAEWILFESVTGLNLIGRGTFDGQGADTWKYNDCSKHP 180

Query: 181 QCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVH 240
           QC +PPTSIKFNKVT G MEG TSVNSKAFHIFVVLSHNIKINN+HIVAPGNSPNTDGVH
Sbjct: 181 QCIIPPTSIKFNKVTQGLMEGITSVNSKAFHIFVVLSHNIKINNVHIVAPGNSPNTDGVH 240

Query: 241 ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGI 300
           ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKY+DEKEVRGI
Sbjct: 241 ISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYRDEKEVRGI 300

Query: 301 FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQ 360
           FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQ+IVLDKVRNPIIIDQ YGSK K+SPSQ
Sbjct: 301 FVSNCTIRNTTNGVRIKTWAASPPGQATRITFQNIVLDKVRNPIIIDQNYGSKTKKSPSQ 360

Query: 361 VKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQPFGNSCLNAK 420
           VK+SDVQFKNIRGTTISPVAVSLQCSAALPCD IKLEQIDVAFSG+  KQPF NSCLNAK
Sbjct: 361 VKVSDVQFKNIRGTTISPVAVSLQCSAALPCDGIKLEQIDVAFSGTHLKQPFANSCLNAK 420

Query: 421 ITTIGKQNPPACV 429
           ITTIGKQNPPACV
Sbjct: 421 ITTIGKQNPPACV 433

BLAST of IVF0008035 vs. NCBI nr
Match: XP_038899827.1 (exopolygalacturonase clone GBGE184 [Benincasa hispida])

HSP 1 Score: 736 bits (1899), Expect = 1.21e-265
Identity = 362/432 (83.80%), Postives = 388/432 (89.81%), Query Frame = 0

Query: 1   MAITERLHIA--AARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTP 60
           MAI ER HIA  A RGL+LGLA LS MA CSSA+A DY GDF GI+ RFL+GAAPAT TP
Sbjct: 1   MAIPERFHIATTAVRGLVLGLAWLSCMAWCSSAVAPDYYGDFAGIKGRFLVGAAPATPTP 60

Query: 61  TAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDG 120
           TAKVFDI  +GAKADG T STQAFMQTWV ACHSGGPAKVVFP GTFLTGPLVYAGPCDG
Sbjct: 61  TAKVFDIRTYGAKADGTTISTQAFMQTWVAACHSGGPAKVVFPAGTFLTGPLVYAGPCDG 120

Query: 121 PMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQC 180
           PMTVEIQGTVKATTDITEYSS EWILFES+T L+LIG GTFDGQGAE W YNDC KH +C
Sbjct: 121 PMTVEIQGTVKATTDITEYSSAEWILFESITGLSLIGSGTFDGQGAETWMYNDCRKHSRC 180

Query: 181 TLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHIS 240
            LPPTSIKFNKVT+G MEG +S+NSKAFHIFVVLSHNIK+NN+HI APGNSPNTDG+HIS
Sbjct: 181 VLPPTSIKFNKVTNGLMEGISSLNSKAFHIFVVLSHNIKVNNLHITAPGNSPNTDGIHIS 240

Query: 241 QTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFV 300
           QTDVVN+TNSIIGTGDDCVSIGHGSTNI V N+TCGPGHGISVGSLGKY DEKEVRGIFV
Sbjct: 241 QTDVVNITNSIIGTGDDCVSIGHGSTNITVSNVTCGPGHGISVGSLGKYDDEKEVRGIFV 300

Query: 301 SNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVK 360
           SNCTIRNTTNG+RIKTWAASP GQATRITFQDI+LD VRNPIIIDQTYGSKKK++PSQVK
Sbjct: 301 SNCTIRNTTNGLRIKTWAASPAGQATRITFQDIILDSVRNPIIIDQTYGSKKKKAPSQVK 360

Query: 361 ISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSRSKQP-FGNSCLNAKI 420
           ISDV FKNIRGTT+S V VSLQCSAA+PCD IKLE+ID+AF+G+R K P F NSCLNAKI
Sbjct: 361 ISDVVFKNIRGTTVSDVGVSLQCSAAVPCDGIKLEEIDLAFTGTRLKHPTFANSCLNAKI 420

Query: 421 TTIGKQNPPACV 429
           T IGKQNPPACV
Sbjct: 421 TAIGKQNPPACV 432

BLAST of IVF0008035 vs. NCBI nr
Match: XP_022953710.1 (exopolygalacturonase clone GBGE184 [Cucurbita moschata])

HSP 1 Score: 635 bits (1638), Expect = 8.23e-226
Identity = 314/437 (71.85%), Postives = 357/437 (81.69%), Query Frame = 0

Query: 1   MAITERLHIAAA----RGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPAT- 60
           MAI  R HIA A    RG++LGLA LS +ARCSS  A  +SGDF+ +R RFL   AP+T 
Sbjct: 1   MAIAGRFHIAVAASAVRGVVLGLAWLSCVARCSSTPAPHFSGDFLPLRGRFLANTAPSTP 60

Query: 61  ---TTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVY 120
              TTP  KVFDI  +GAK+DG+  +TQAFM+ WV+ACHSGGPAKVVFPPG FLTGPLVY
Sbjct: 61  STPTTPGTKVFDITTYGAKSDGKKINTQAFMKAWVEACHSGGPAKVVFPPGKFLTGPLVY 120

Query: 121 AGPCDGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDC 180
           AGPCD PMTVEIQGTV ATTDITEYSSP WILFES+T LNLIG GTFDGQG E W YNDC
Sbjct: 121 AGPCDAPMTVEIQGTVLATTDITEYSSPVWILFESITGLNLIGNGTFDGQGQETWMYNDC 180

Query: 181 SKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNT 240
             +P C +PPTS+KFNKVT+G MEG +S+NSK FHIF+VLSH++++NN++I+AP  SPNT
Sbjct: 181 RDNPSCIMPPTSLKFNKVTNGLMEGISSLNSKGFHIFLVLSHHLRVNNLNIIAPDQSPNT 240

Query: 241 DGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKE 300
           DG+HISQTD V +T+SIIGTGDDCVSIGHGS N+ V NI CGPGHGISVGSLGKYKDEK+
Sbjct: 241 DGIHISQTDNVTITSSIIGTGDDCVSIGHGSVNVTVSNIKCGPGHGISVGSLGKYKDEKD 300

Query: 301 VRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKR 360
           V GIFVSNCT++NTT GVRIKTWA SPP QAT I FQDIVLD V++PIIIDQ YGSKK +
Sbjct: 301 VSGIFVSNCTLKNTTFGVRIKTWADSPPSQATGIHFQDIVLDSVKSPIIIDQFYGSKKNK 360

Query: 361 SPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS-RSKQPFGNS 420
            PS+VKISDV +KNIRGTTIS V VSLQCS+A+PCD I LE ID+AF GS R K   GNS
Sbjct: 361 KPSKVKISDVSYKNIRGTTISVVPVSLQCSSAVPCDGITLEDIDLAFIGSDRKKALVGNS 420

Query: 421 CLNAKITTIGKQNPPAC 428
           CLNAK    GKQNPPAC
Sbjct: 421 CLNAKFKATGKQNPPAC 437

BLAST of IVF0008035 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 375.6 bits (963), Expect = 5.5e-104
Identity = 195/427 (45.67%), Postives = 273/427 (63.93%), Query Frame = 0

Query: 9   IAAARGLILGLALLSSMARCSSAIASDYSGDFVGIRSRFLIGAAPATTTPTAKVFDIIAH 68
           +A A  + +G  +L ++    S +A   +G+++G R     G A  +  PT  V+DI   
Sbjct: 8   VAKANNINVGSLILMALV-FGSCVA---NGEYLGGRR----GLAANSGNPT--VYDITKF 67

Query: 69  GAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTGPLVYAGPCDGPMTVEIQGTV 128
           GA  DG T++ +AF+ TW++ C S  PA ++ P GTFL GP+++AGPC   +TV + GT+
Sbjct: 68  GAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVTVNVIGTI 127

Query: 129 KATTDITEYSSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLPPTSIKFN 188
            ATT  + Y++PEW LFE V  L L G GTF G+G   WK + C K  QC LPPTS+KF 
Sbjct: 128 IATT--SGYATPEWFLFERVDNLVLTGTGTFHGKGEAVWKADGCGKKVQCNLPPTSLKFR 187

Query: 189 KVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTDVVNVTNS 248
            + +  + G +SVN+KAFH+F+V + N+ I NI + AP  SPNTDG+H+S  D V++ +S
Sbjct: 188 NMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHLSNADNVSILDS 247

Query: 249 IIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNCTIRNTTN 308
            I TGDDCVS+G GS N+ V  + CGPGHG+SVGSLGKYK+E++V GI V+NCT+  T N
Sbjct: 248 TIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIHVNNCTMIETDN 307

Query: 309 GVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQTYGSKKKRSPSQVKISDVQFKNIR 368
           G+RIKTW  S P +A  I F++I++  V+NPIIIDQ YGS  +   SQV ISD+ FKNIR
Sbjct: 308 GLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNYGS--RGGDSQVAISDILFKNIR 367

Query: 369 GTTISPVAVSLQCSAALPCDSIKLEQIDVAFSG-------SRSKQPFGNSCLNAKITTIG 428
           GTTI+   V + CS ++PC  + +  +++ + G       S S    G  C NA +   G
Sbjct: 368 GTTITKDVVQIMCSKSVPCQGVNVVDVNLDYVGKTGGEKKSSSGGLVGALCDNANVIFGG 420

BLAST of IVF0008035 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 328.9 bits (842), Expect = 5.9e-90
Identity = 173/385 (44.94%), Postives = 235/385 (61.04%), Query Frame = 0

Query: 49  IGAAPATTTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTG 108
           +GA      P     D+ A GAK DG+TD + AF   W +AC +G  + +  P G +L  
Sbjct: 57  VGAKVTGAKPGGASLDVKASGAKGDGKTDDSAAFAAAWKEACAAG--STITVPKGEYLVE 116

Query: 109 PLVYAGPCDGPMTVEIQGTVKATTDI-TEYSSPEWILFESVTKLNLIG-KGTFDGQGAEA 168
            L + GPC GP+T+E+ G  KA   + T      WI FE++    L G K  FDGQG+ A
Sbjct: 117 SLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENLADFTLNGNKAIFDGQGSLA 176

Query: 169 WKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAP 228
           WK NDC+K  +C   P +I+F  +T+  +   TS NSK FH+ ++   NI + +I I AP
Sbjct: 177 WKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLTDIGIDAP 236

Query: 229 GNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGK 288
             S NTDG+HI +++ VN+  + I TGDDCVSIG G+ N+ V N+ CGPGHGIS+GSLG+
Sbjct: 237 PESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGR 296

Query: 289 YKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQ-- 348
           Y +E+ V+G+ V  C I+NT NGVRIKTW  SPPG A+ I F+DI +D V  P++IDQ  
Sbjct: 297 YPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEY 356

Query: 349 -TYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSR 408
             YG  K   PS+VK+SDV  KNI+GT+ + VAV L CS  +PC +I L  I++  +G  
Sbjct: 357 CPYGHCKAGVPSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKE 416

Query: 409 SKQPFGNSCLNAKITTIGKQNPPAC 429
              P  ++C N K    GK  P AC
Sbjct: 417 G--PAVSACSNIKPILSGKLVPAAC 437

BLAST of IVF0008035 vs. TAIR 10
Match: AT3G07850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 325.9 bits (834), Expect = 5.0e-89
Identity = 173/385 (44.94%), Postives = 237/385 (61.56%), Query Frame = 0

Query: 49  IGAAPATTTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAKVVFPPGTFLTG 108
           +GA  +   P A V D+ A GAK D +TD + AF   W +AC +G  + +  P G ++  
Sbjct: 57  VGAKVSGAKPGAAV-DVKASGAKGDSKTDDSAAFAAAWKEACAAG--STITVPKGEYMVE 116

Query: 109 PLVYAGPCDGPMTVEIQGTVKATTDI-TEYSSPEWILFESVTKLNLIG-KGTFDGQGAEA 168
            L + GPC GP+T+E+ G  KA   + T      WI FE++    L G K  FDGQG+ A
Sbjct: 117 SLEFKGPCKGPVTLELNGNFKAPATVKTTKPHAGWIDFENIADFTLNGNKAIFDGQGSLA 176

Query: 169 WKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAP 228
           WK NDC+K  +C   P +I+F  +T+  +   TS NSK FH+ ++   NI +++I I AP
Sbjct: 177 WKANDCAKTGKCNSLPINIRFTGLTNSKINSITSTNSKLFHMNILNCKNITLSDIGIDAP 236

Query: 229 GNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGK 288
             S NTDG+HI +++ VN+  + I TGDDCVSIG G+ N+ V N+ CGPGHGIS+GSLG+
Sbjct: 237 PESLNTDGIHIGRSNGVNLIGAKIKTGDDCVSIGDGTENLIVENVECGPGHGISIGSLGR 296

Query: 289 YKDEKEVRGIFVSNCTIRNTTNGVRIKTWAASPPGQATRITFQDIVLDKVRNPIIIDQ-- 348
           Y +E+ V+G+ V  C I+NT NGVRIKTW  SPPG A+ I F+DI +D V  P++IDQ  
Sbjct: 297 YPNEQPVKGVTVRKCLIKNTDNGVRIKTWPGSPPGIASNILFEDITMDNVSLPVLIDQEY 356

Query: 349 -TYGSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGSR 408
             YG  K   PSQVK+SDV  K I+GT+ + VAV L CS  +PC +I L  I++  +G  
Sbjct: 357 CPYGHCKAGVPSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINLVHNGKE 416

Query: 409 SKQPFGNSCLNAKITTIGKQNPPAC 429
              P  ++C N K    GK  P AC
Sbjct: 417 G--PAVSACSNIKPILSGKLVPAAC 436

BLAST of IVF0008035 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 323.2 bits (827), Expect = 3.2e-88
Identity = 171/380 (45.00%), Postives = 236/380 (62.11%), Query Frame = 0

Query: 64  DIIAHGAKADGRTDSTQAFMQTWVKACHSGGPA-KVVFPPGTFLTGPLVYAGPCDGPMTV 123
           D+ + GA+A+   D T+AF+  W KAC S   +  ++ P G F  G L ++GPC     +
Sbjct: 36  DVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSNL 95

Query: 124 EIQGTVKATTDITEY-SSPEWILFESVTKLNLIGKGTFDGQGAEAWKYNDCSKHPQCTLP 183
            ++  VKA+TD+++Y S   WI F  +  L L G GTFDGQGA AW +N+C+    C L 
Sbjct: 96  TVR--VKASTDLSKYRSGGGWIQFGWINGLTLTGGGTFDGQGALAWPFNNCTSDSNCKLL 155

Query: 184 PTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIKINNIHIVAPGNSPNTDGVHISQTD 243
           PTS+KF  +    +   +SVNSK FHI +V   + K   ++I AP +SPNTDG+HI ++ 
Sbjct: 156 PTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIERSS 215

Query: 244 VVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGHGISVGSLGKYKDEKEVRGIFVSNC 303
            V  + S I TGDDCVSIG G++ I + +I CGPGHGISVGSLG+Y +EK+V G+ V +C
Sbjct: 216 NVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVKDC 275

Query: 304 TIRNTTNGVRIKTWAASPP-GQATRITFQDIVLDKVRNPIIIDQTY---GSKKKRSPSQV 363
            I  TTNG+RIKTWA SP    AT +TF++I+++ V NPIIIDQ+Y    S     PS+V
Sbjct: 276 KISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNVPSKV 335

Query: 364 KISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLEQIDVAFSGS---------RSKQPF 423
           ++S++ FKNIRGT+ S VAV L CS  +PC  + LE + +  S S         R  +  
Sbjct: 336 ELSEIYFKNIRGTSSSLVAVQLHCSRGMPCKKVYLENVHLDLSSSDGGRKQSSNRGNEAV 395

Query: 424 GNSCLNAKITTIGKQNPPAC 429
            +SC N +   IG Q PP C
Sbjct: 396 SSSCRNVRANYIGTQIPPPC 413

BLAST of IVF0008035 vs. TAIR 10
Match: AT3G07820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 313.5 bits (802), Expect = 2.6e-85
Identity = 163/395 (41.27%), Postives = 242/395 (61.27%), Query Frame = 0

Query: 38  GDFVGIRSRFLIGAAPATTTPTAKVFDIIAHGAKADGRTDSTQAFMQTWVKACHSGGPAK 97
           G + G+ + F+I       +  A+VF I      +   +D TQA ++ +  AC S  P+K
Sbjct: 2   GGYFGVSTIFII--CLLGISANAEVFTI-----GSSSGSDITQALLKAFTSACQSSSPSK 61

Query: 98  VVFPPGTFLTGPLVYAGPCDGPMTVEIQGTVKATTDITEYSSPEWILFESVTKLNLIGKG 157
           VV P G F  G +   GPC  P+ V +QGTVKA  +  +    +W++F ++    L G G
Sbjct: 62  VVIPKGEFKLGEIEMRGPCKAPIEVTLQGTVKADGNAIQ-GKEKWVVFGNIDGFKLNGGG 121

Query: 158 TFDGQGAEAWKYNDCSKHPQCTLPPTSIKFNKVTHGSMEGFTSVNSKAFHIFVVLSHNIK 217
            FDG+G  AW+ N+C K  +C   P SI+F+ + +  +   +S+++K FHI V+ + N+ 
Sbjct: 122 AFDGEGNAAWRVNNCHKTFECKKLPISIRFDFILNSEIRDISSIDAKNFHINVLGAKNMT 181

Query: 218 INNIHIVAPGNSPNTDGVHISQTDVVNVTNSIIGTGDDCVSIGHGSTNINVLNITCGPGH 277
           +NNI IVAP +SPNTDG+H+ ++D V + NS I TGDDC+S+G G  N++V  +TCGPGH
Sbjct: 182 MNNIKIVAPEDSPNTDGIHLGRSDGVKILNSFISTGDDCISVGDGMKNLHVEKVTCGPGH 241

Query: 278 GISVGSLGKYKDEKEVRGIFVSNCTIRNTTNGVRIKTW-AASPPGQATRITFQDIVLDKV 337
           GISVGSLG+Y  E++V GI V NCT++ T NG+RIKTW +A+    A+ I F+DI+L  V
Sbjct: 242 GISVGSLGRYGHEQDVSGIKVINCTLQETDNGLRIKTWPSAACSTTASDIHFEDIILKDV 301

Query: 338 RNPIIIDQTY---GSKKKRSPSQVKISDVQFKNIRGTTISPVAVSLQCSAALPCDSIKLE 397
            NPI+IDQ Y       K+  S +K+ ++ FKNIRGT+ +  AV L CS   PC ++++ 
Sbjct: 302 SNPILIDQEYCPWNQCNKQKASTIKLVNISFKNIRGTSGNKDAVKLLCSKGYPCQNVEIG 361

Query: 398 QIDVAFSGSRSKQPFGNSCLNAKITTIGKQNPPAC 429
            ID+ ++G+     F   C N     +G Q+P AC
Sbjct: 362 DIDIKYNGADGPATF--HCSNVSPKILGSQSPKAC 386

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P490627.7e-10345.67Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ... [more]
Q397661.4e-9144.86Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
Q6H9K08.9e-9143.67Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
Q397861.5e-9044.32Polygalacturonase OS=Gossypium hirsutum OX=3635 GN=G9 PE=2 SV=1[more]
P490637.0e-8844.94Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ... [more]
Match NameE-valueIdentityDescription
A0A5D3D1R65.9e-247100.00Exopolygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411... [more]
A0A1S3BRS35.9e-247100.00exopolygalacturonase clone GBGE184 OS=Cucumis melo OX=3656 GN=LOC103492794 PE=3 ... [more]
A0A5A7TXI91.7e-24699.77Exopolygalacturonase clone OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A0A0K9C45.9e-22389.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G404800 PE=3 SV=1[more]
A0A6J1GQF23.4e-17871.85exopolygalacturonase clone GBGE184 OS=Cucurbita moschata OX=3662 GN=LOC111456159... [more]
Match NameE-valueIdentityDescription
XP_008451547.10.0100.00PREDICTED: exopolygalacturonase clone GBGE184 [Cucumis melo] >TYK18191.1 exopoly... [more]
KAA0046656.11.79e-31599.77exopolygalacturonase clone [Cucumis melo var. makuwa][more]
XP_011659369.11.62e-28489.38exopolygalacturonase clone GBGE184 [Cucumis sativus] >KGN44972.1 hypothetical pr... [more]
XP_038899827.11.21e-26583.80exopolygalacturonase clone GBGE184 [Benincasa hispida][more]
XP_022953710.18.23e-22671.85exopolygalacturonase clone GBGE184 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G02790.15.5e-10445.67polygalacturonase 4 [more]
AT3G14040.15.9e-9044.94Pectin lyase-like superfamily protein [more]
AT3G07850.15.0e-8944.94Pectin lyase-like superfamily protein [more]
AT4G18180.13.2e-8845.00Pectin lyase-like superfamily protein [more]
AT3G07820.12.6e-8541.27Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 293..314
e-value: 0.51
score: 19.4
coord: 263..283
e-value: 8400.0
score: 0.6
coord: 354..376
e-value: 7000.0
score: 1.2
coord: 323..344
e-value: 5000.0
score: 2.2
coord: 213..239
e-value: 16.0
score: 14.4
coord: 240..261
e-value: 5000.0
score: 2.3
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 50..427
e-value: 3.0E-113
score: 380.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 90..403
e-value: 3.2E-91
score: 305.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 270..283
NoneNo IPR availablePANTHERPTHR31375:SF158POLYGALACTURONASEcoord: 19..425
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 19..425
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 51..429

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0008035.2IVF0008035.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity