IVF0007934 (gene) Melon (IVF77) v1

Overview
NameIVF0007934
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptiontRNA-5-taurinomethyluridine 2-sulfurtransferase
Locationchr05: 1014303 .. 1018883 (-)
RNA-Seq ExpressionIVF0007934
SyntenyIVF0007934
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTCTGTTATGTTATATGGTTCTTGTGTTTTCTTGCAAATTATGGTAGCTGATATAGTGAATGCTGGTGCAATTGTGAGCAATCAAATGGGCATTTTCAGCTCGAAGGAAATATAGCCTTTTCGTGTGAACGGACTTTAATGGGTTCTGTTTTCGTAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCTTATAAATCCATCTAAACTTGCAAAGGTTAATTTTTCCAACGAATTTTCAAATGAAATAAAGGATTTGTTGGGAAAATTAAACATTATTCAAGATACCCACAAGTAATGTCTTTCTTCTGTAGTTTGTGCTCAAGAGAGATCTTTGAGCTGAAAATGTTATGTAAATTGGTTTTCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTAAGTGCATTTACAACTACAATTTTATCCTATAACTTTGTTGGATGTGTTAACGATATATTAATACATCCAATCAATTTACTTGTAGCTGTTCCAGCTGACCATCTTTCTCATTCATTCTTTTCTAGTAGCACAAACTGGTATTCGGTTCTCATTTATGTCTACTGAATACTGTCTATTTTATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCGATGTCCTTTGTAACACAAGAATAAAGTTTGGTATGTTCATTAGATGGACATTGGTTATATCGTCAAGCTTTCTATTAATGTTTATGATTTCTTTTGTATTAATCTAATATATGGCAAGCCCTGCTTTTTCCAGGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTATCTGGGAACGATTGCACAGACATTTCTTTTAATCTAACCTGTCCATTAGGGAGTATCGGTTATTCCTTGCTTAACTTTAATATTTTGTGGTAGGGAAAGGGATCATAGTGAGATTTGGTCTTTCATTAGATTTCATGTGTCTCTTTGAGCTTTGATTTCAAAGTCCTTTTTTAATTATTCAATAGGTGGTATCTTTCTCAGTTGGTCCCCCTTTCTTTAGTTAGGGTGTTTTCTGTGGGTTGTTTTTTTTGTATGCCCTTGTATTCTTGTGATTTCTCATGAAAAAATTGTAGTGGATTGTTTTTGTAACCCTTTAGGTAGGACACTATTTTGCTATTTTCATGACTTTTTTCTATATTCTACACTACACATGGCAGTCAGTAACGCCTATAGTAATTTAACTTGAAGTTTCATGTATAAAGTCAGTGGAATAGATATGACTAATATTGCATTATACGGCCACTATTTGTTGCAATCCTTCAGGAAACAACATCATTAATAAGCTTTATGATTTATATATCGCTTACCTAGTTCAAGGTTTATCATCAAACACATTTATCCCTTCCTTGTTCCTTCTTGCTCTAAAATAAAGATTTCTTATTTTACTTGATTATTATTGTTCTTAGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGTTTGTTCAAGCTAATTTTATAGTATTTATTATGATGTATTTTTTTGAGGTATCTCTTATGTTTCAGACTTCAGGGTGTATTCCGAGATGTTCAAGTTCAACTATTGTAAATTTGTACAATTTGGTACTGGCCTTCTAAGCTTTGTACAGACATTAACACCTAGTGGCTAACATTGGTTGCATTCAGTTGAGAAATTTATGTATTTAATAAAAGCTAAAAACTGTATTACAGACTTTCGCAAGCCAAATGATGTAGATAACATTTTAGAGATTTGTGGATGTTATATTGCAGTTTGAACCCATTTAGTTTTTACTATTCTCGTGTGCAAAACTTATATAAGTGCCTCAATTTATTAGTTCATATCATGCCAACTCCAATCCCAACTAGCTATATTCTTTCAACTTATGCTCTATCTTTTCTGAAGGAAAAAGGAATACTTGTGTGCTTGTTATCTTGTTAATATGTTTTTCTAGGGTTGTGACTTCAGCAATATTTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGGTACTTTGGATGTGGTGATTCATACATTTTTTTTTTTGTTTGTTTGTCTTTTTTCACGGGAGGCTAATGACTTTCTCAAAATTCATTGGTCAATGCTGATCTATTTCTAATATTTTGGTGTTGCTACTTTATATTTTAGAATATTCTTTACTACTTTTTAGAACAATTTATAGTAACATAGGCTGGCTTAATGGATTTAGTACAAGATTTGCCAACTCTTGTTGAAATTTTCTGATGGCTATACTTTTCCTGAGCAAGTGTTTTATTTCCTTATTCTTCATGACAGCAACAATTTTCATTCATCCTGTTTCTGAAGATGGGTCTCCTGTTTAAGGTATCTTGTGGTAGAATTCATGTAGAACCTGTTTCTTTGATGTGTAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTCTCAGTTTCTTTTCTTTAAGAATAAATTTGATGATCTCTAGCTGTTGTATGGCCATTTTACCAAAACTTCAAGAACATCTTCAACTCTATTTGGTGCAGAGATTTGAGCATTCCGATGTGCCAGTTTAGTTTTTAGTGACCGATTACATTGGGATTATTTCCAATCTGATGCTTCACAAGCTCACATTCTTTACTTACGTCTCTTGCTGAGCTAACAATGACGATTTCAGCTTTTTTGCCTTCGTCTAATGCAGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTATAGTTTCAGCTTCCATCTCCCATATTTCTTGTTAGTTTCACTACTTCAAGTTGGCCTGGCCATAACGATTTACCATAAAAGGAAAATTTCTTAACTTTTTTACAAACCTTTCCAGAGAACCCATCAGTTTATTCCTGCAAGTTTGTGTTGGAAGTTTGACAAGCATGCTTGGCAATATTGATAATCCGGAAATGTTGACTATTACATAGAAAAGTAATTTATCTTCCATGATTATTTAATAGAACAAGCATCTCGATATCATCGATTTTTCTTTCAAACAAAGGATTTAAGTTTAGAATTCAATTTTATCTGTTAAGGGACTTTTTTTGGGGGCCTTTCTCTTTGAGAGGGGAAAATGTATTAAGATTAAGTAGATCTAGAAATGTTATGGGGAGGCTTTGTCTGCTATATCCCGCCCATTTGCTTTGAGATTGTGTAATTGGACTCGAGTTCTCATCTGTCATTTCCAGTTCATTGTTTCATAATTTTATTCTATTTCTAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAG

mRNA sequence

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAG

Coding sequence (CDS)

ATGATGCTGAGACTGAGAGTGAATCCCGCCGTGTGGTGCTCTGTTCCGATTTCGCATTTCACCTCTCTTCCCCTTTCACCCTCGATTCTCAGACCTCTCCTCCGCCCCCACAACCCTCGTCTCTTCTTCACCCGATTCTCCGCTTCCTCTGCCGTGCAGCATAATACCTCCCAATCTTCTTTACCCAAACCCCTCTCCGATGCCGACTTCCTCCCTTTCCTCTCATGTTCGATGCCTACTTATCCCCTCAAAGTCGCCGTTCTTCTCAGCGGCGGCGTCGATAGTAGCGTCGCACTCCGCCTTCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGACTTCGAGAATTTTTGGTCAGAATGTCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGATTGATGTACCGTTGGAAGTAGTTCATTTGACAGAGGAATATTGGAGGAACGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGAGCATTCATGGATGCGATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCGAATGTTATTCACCCATCTGCGGATCAAATGGATAAACCTTCTGTTTTAGAACTGTCCAAGGATATGGTCAAGGATCAAACGTATTTTCTATCACATCTCTCACAGAATCAACTTAAACGGCTTTTATTCCCTCTCGGTTGTATTCCAAAGGATGAGGTTCGCAAGCTTGCTGCAAAATTCAACCTACCAAACAAAGACAGAAAGGATTCACAAGGAATATGTTTCCTTGGGAAGATAAAATTTAGTGAATTTGTTGCAAGACATATTGGGGAGGATGAAGGTGTGATATTAGAGGCAGAGTCGGGAGATTTCCTTGGCAAACATCGAGGATTTTGGTTCTACACTATGGGCCAACGCCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTCGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAACTATTTCTCATTTGACAAAAGGAGGCGGGTGTTTCGTGTTGGATCTCTAAAATGGCTTAGTGGTATGCCTCCAGACCGATTTACTGGGCTTCAATGTAAGGTCCGACATGGCCCGGGTTTCTATAGTTGCAATTTGACAATGGAACACAATAAGGATGGTCATGAAGATGTTGCAGTCGTCCACCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGCCAGTTTGCCGCCTTTTATAAGGGAAGGAGCTGCATTGGGTCAGGTGTGATTTTAGAATCATGGGATGATCAAGGGTTTCCTGTATGTCAAAAGGCTATTGAAAATGCACAAATGGAAGATAAATCGAAACTTGGCAAACCAATAAAGATAAAAGTGAAACCAGACAGTGGTATGAACGAATCTAAAGCCAAAGATGATACAAAAATACAAGACAAGTCAAGGAACGAAGAAGCCACTGTGGTTGAGCAAATGAGAAAAGTATCTCCAGATGAAGCAATGGCCACAACCCCTATGAAATGGCTTGGAAGCCTTAGGAAGAAGTTGCTGCAGATATTTTAG

Protein sequence

MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSLPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Homology
BLAST of IVF0007934 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 328.6 bits (841), Expect = 1.3e-88
Identity = 159/365 (43.56%), Postives = 236/365 (64.66%), Query Frame = 0

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------------AFMDAISNMEFD 202
           C + +VP E+++  +EY+  VVSY I+E + G                AF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 432
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

BLAST of IVF0007934 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.7e-88
Identity = 157/365 (43.01%), Postives = 237/365 (64.93%), Query Frame = 0

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------------AFMDAISNMEFD 202
           C++ ++P E+++  +EY+  VV Y IEE + G                AF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++GMP D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 432
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

BLAST of IVF0007934 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.9e-88
Identity = 157/365 (43.01%), Postives = 237/365 (64.93%), Query Frame = 0

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------------AFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G                AF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 432
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

BLAST of IVF0007934 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 2.9e-88
Identity = 157/365 (43.01%), Postives = 237/365 (64.93%), Query Frame = 0

Query: 83  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------------AFMDAISNMEFD 202
           C++ +VP E+++  +EY+  VVSY IEE + G                AF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W++  P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 432
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

BLAST of IVF0007934 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 314.3 bits (804), Expect = 2.6e-84
Identity = 155/366 (42.35%), Postives = 231/366 (63.11%), Query Frame = 0

Query: 83  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 142
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 143 CDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------------AFMDAISNMEFD 202
           C + +VP E++ L +EY++ VV+Y IEE + G                AF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 203 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 262
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 263 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 322
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 323 FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 382
           F+T+GQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W++   P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 383 FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 433
              L  K+RHG     C + M  N     D+  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

BLAST of IVF0007934 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 9.5e-308
Identity = 524/539 (97.22%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 525
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of IVF0007934 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1065.1 bits (2753), Expect = 9.5e-308
Identity = 524/539 (97.22%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 525
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of IVF0007934 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.4e-306
Identity = 521/539 (96.66%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPD+FTGLQCKVRHGPGFYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 525
           PDSGMNESKAKDDTKIQD+SRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of IVF0007934 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 3.6e-291
Identity = 499/539 (92.58%), Postives = 510/539 (94.62%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 525
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

BLAST of IVF0007934 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.2e-265
Identity = 457/538 (84.94%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------- 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGR       
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 --------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
                   AFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 525
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

BLAST of IVF0007934 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1062 bits (2747), Expect = 0.0
Identity = 524/539 (97.22%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 524
           PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of IVF0007934 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 521/539 (96.66%), Postives = 524/539 (97.22%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
           LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSGMPPD+FTGLQCKVRHGPGFYSCNLTMEHN+DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 524
           PDSGMNESKAKDDTKIQD+SRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF
Sbjct: 481 PDSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

BLAST of IVF0007934 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1007 bits (2604), Expect = 0.0
Identity = 499/539 (92.58%), Postives = 510/539 (94.62%), Query Frame = 0

Query: 1   MMLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSS 60
           MMLRLRVNPAVWCSVPISHFTSLPL+PSILRPLLR HNPRLF TRFSASSAV HNTS SS
Sbjct: 1   MMLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAV-HNTSLSS 60

Query: 61  LPKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120
             KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE
Sbjct: 61  SSKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE 120

Query: 121 DFENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------ 180
           DFENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGR      
Sbjct: 121 DFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDV 180

Query: 181 ---------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240
                    AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS
Sbjct: 181 LCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLS 240

Query: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300
           HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE
Sbjct: 241 HLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGE 300

Query: 301 DEGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360
           DEGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD
Sbjct: 301 DEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFD 360

Query: 361 KRRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSED 420
           KRRRVFRVGSLKWLSG PPD+FT LQCKVRHGP FYSCNLTMEH++DGHEDVAVVHLSED
Sbjct: 361 KRRRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSED 420

Query: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480
           DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK
Sbjct: 421 DQGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVK 480

Query: 481 PDSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 524
           P+SGM ESKAKDDT+I DK RNEEATVVEQ+R+VSPDEAMAT  MKWLGSL+KKLLQIF
Sbjct: 481 PESGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

BLAST of IVF0007934 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 962 bits (2487), Expect = 0.0
Identity = 472/538 (87.73%), Postives = 497/538 (92.38%), Query Frame = 0

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALC-KTLESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------- 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGR       
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 --------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
                   AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRRVFRVGSLKWL+GMPPD+F+GLQCKVRHGP FYSCNLTMEHN+DG ED+AVVHLSEDD
Sbjct: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 524
           +S + ES+AKDDTKI  +S N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

BLAST of IVF0007934 vs. NCBI nr
Match: XP_022944568.1 (uncharacterized protein LOC111448983 [Cucurbita moschata])

HSP 1 Score: 923 bits (2385), Expect = 0.0
Identity = 457/538 (84.94%), Postives = 483/538 (89.78%), Query Frame = 0

Query: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61
           MLRLRVNP VWC++PISHFTSLPL PSILRP+LR H PRLF TRFSASSAV H  S+SS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAV-HKASESSS 60

Query: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121
            K LSD D  PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 61  AKGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120

Query: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR------- 181
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGR       
Sbjct: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180

Query: 182 --------AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241
                   AFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSH
Sbjct: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSH 240

Query: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300

Query: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361
           EGVILEAESGDFLGKHRGFWFYT+GQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DK
Sbjct: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDK 360

Query: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421
           RRR+FRVGSLKWLSG+ PD+FT LQCKVRHGP FYSCNLTME N+D  EDVAVVHLSEDD
Sbjct: 361 RRRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDD 420

Query: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481
           QGLAAGQFAAFY+GR CIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKP
Sbjct: 421 QGLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKP 480

Query: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 524
           ++   E + KDDTKI    +N++ TVVEQ  +V+ DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 481 ENATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

BLAST of IVF0007934 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 671.8 bits (1732), Expect = 4.5e-193
Identity = 337/477 (70.65%), Postives = 379/477 (79.45%), Query Frame = 0

Query: 28  SILRPLLRPHNP-------------RLFFTRFSASSAVQHNTSQSSLP-KPLSDAD---- 87
           ++ +P L P  P             R+F + FS+SS+ Q  + QSS P  PL  ++    
Sbjct: 4   AVAKPFLSPSPPLFRLPLSSISISFRVFPSFFSSSSSSQLPSPQSSNPITPLISSNRNYS 63

Query: 88  ---FL--PFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFEN 147
              F+   +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FEN
Sbjct: 64  RHAFIVDQYLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFEN 123

Query: 148 FWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGR---------- 207
           FW++CPW+DDLKYAK VC+Q+DVPLEVVHLT+EYW  VVSYIIEEYRCGR          
Sbjct: 124 FWNQCPWEDDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNT 183

Query: 208 -----AFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQ 267
                AFMDAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ
Sbjct: 184 RIKFGAFMDAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQ 243

Query: 268 NQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGV 327
            QLKRLLFPLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+
Sbjct: 244 TQLKRLLFPLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGI 303

Query: 328 ILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRR 387
           ILEAESGDFLG HRGFWFYT+GQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR
Sbjct: 304 ILEAESGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRR 363

Query: 388 VFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGL 447
           +FRVGSL+W SG P  +   L+CKVRHGPGFYSC+  ME    G  DVAVVHL EDDQGL
Sbjct: 364 IFRVGSLRWNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGL 423

Query: 448 AAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 467
           AAGQFAAFY+G +CIGSGVILESWDDQ FPVC KA++ A +EDK+KLGKP+KI   P
Sbjct: 424 AAGQFAAFYEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMP 476

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0SMH11.3e-8843.56tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN... [more]
Q660I81.7e-8843.01tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496... [more]
O516252.9e-8843.01tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... [more]
B7J2N72.9e-8843.01tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... [more]
B2S1252.6e-8442.35tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... [more]
Match NameE-valueIdentityDescription
A0A5D3B8G89.5e-30897.22tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BNL89.5e-30897.22tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5A7TDN62.4e-30696.66tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
A0A0A0KX163.6e-29192.58tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A6J1FY631.2e-26584.94tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
XP_008449720.10.097.22PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp... [more]
KAA0041410.10.096.66tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa][more]
XP_004142116.10.092.58uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy... [more]
XP_038901654.10.087.73tRNA-specific 2-thiouridylase MnmA [Benincasa hispida][more]
XP_022944568.10.084.94uncharacterized protein LOC111448983 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G51310.14.5e-19370.65transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023382Adenine nucleotide alpha hydrolase-like domainsGENE3D2.30.30.280coord: 282..341
e-value: 3.3E-18
score: 66.8
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 84..429
e-value: 1.2E-105
score: 353.3
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 348..437
e-value: 2.7E-13
score: 51.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 464..494
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 72..462
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 84..314
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 75..281
e-value: 3.5E-67
score: 228.0
IPR004506tRNA-specific 2-thiouridylaseTIGRFAMTIGR00420TIGR00420coord: 84..429
e-value: 1.1E-89
score: 298.7
IPR004506tRNA-specific 2-thiouridylaseCDDcd01998tRNA_Me_transcoord: 84..429
e-value: 8.83472E-140
score: 405.367

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0007934.2IVF0007934.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0005524 ATP binding
molecular_function GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function GO:0000049 tRNA binding
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0016740 transferase activity