IVF0006951 (gene) Melon (IVF77) v1

Overview
NameIVF0006951
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionMethylenetetrahydrofolate reductase
Locationchr05: 8979275 .. 8984444 (+)
RNA-Seq ExpressionIVF0006951
SyntenyIVF0006951
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTAACCGACAATGCTCAGATGCGATTTCCTATCGTCATTCCCCTTCTCTTTCACTCATTTCTCTCTTTCCATCTCTCATTCTCCTCTTTTTTTTTTTCTCTTTCGTAAGTCTTCACTTTCGATTCTATCCTTCTTCTACTTTCCCCCATATGTTTCACATTGTTCATTATTTCCATTGATTTCCAGGTTGCTGCTTCCGATCTCTAACTCCTACACCACTCAAGGGAAGAGAAGATGAAGGTGATTGACAAGATCCGGGCAGCCACCACCGGCGATGGCGGAAGAGTTGTTTTCTCCTTTGAGTACTTCCCTCCCAAGACAGAGGATGGTGTCGATAATCTTTTTGAGAGGATGGACCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCTGGTGGCTCCACTGCCGATCTCACCCTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTTGAGACCATGATGCATCTCACTTGCACCAATATGCCTGTTGAGAAGATCGACCACGCTCTTCAGACTATCAAGGCCAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGTGACCCTCCCCATGGACAGGACAAATTTGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTACTCTCTAAACCTTCGTCTGTGATTGTTTTTTGAATGTTAATTGTTTTTGCTCTCTAAATGTTTCCATTTCTTATTCATAGGTTAAACATATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGGTTTTTTTTTTTCTGATCAACAGTTACAGATCTACTTTTGGCTGCCTTTTCTTTTCTGTAACTAACGGAATGCTAATGGTTACTTCCACTCTGTTCTTCTCAGAGGCTCATCCGGATGTGATTGGAAGCAATGGCGTGGCGACGACTGAAGGCTACCAAAGCGATCTGGCTTATCTTAAGAAAAAGGTGACTCTGATCTTAAAGCAATCTTGATTGATCTTTTTCCTGTTCTTACATGGGTTACTTTTGATATGCTACTTTATTTGTTTTCTGCTTGCTGCTATTTTATTATTTTGTTTTGGGTTCTTCTCAGTTCGATGCTGGGGCTGATCTGATTGTTACTCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGTCAAATTGGAATTACTTGCCCAATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACTAAGGTATAACCTTCCTCTGATCTTCATTAATGGTCTTGATATAGGTTATAAGTATTAACACTTGTTCGTCGCACTTCATGATACTTCAAAATTTACAATCGGTGATCATCAATTTTTTATTTTTCTTCTCCAAAGAAAAACTACTTTGATGTATAGTGTTAAATTTTTTCTCTTCTACCTATATGCGTGATTTTTGAAAACCTACAGATTCCAGACGAGATCATGGCTGCCTTAGAGCCTATCAAGGATAATGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGTAAGAAGATTTTGGCTCATGGAATCAAGACTCTGCATATTTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGGTATATTCCAGTATACAATGAATGGCATTCTTGTTTTACTTCAAGATATTGTTTCTGTGGGTAATTTTGTCTGTGGATGCTCTGGTACAAGGGGAAGTTTCTGAAGCTTTTGTTGACGTGTTGTATTGGTGGGTGGTTTCCGTTTATTGAATATGGCTTCCTTTCATTTCTTAATCTTGGTTCATGTTTTATTTGAACAACTGAGAATCTGCATCTACCTTTGAAGTAAACTTGTAAGGAGTCATAAAGTAATTGTTATAATGAAAACTTCTGAATGTTAATTGCTGATACGCCTGTTAGAAGTATGTTTTGAAACACTGCCACAATTATCGTATACTAAGAGGAACATGAGACTCATACACTTCTATACGTTTTAATATAGAATAAATATATTATAACATGAGAGTTTTTTTTTTTCATTGGATGTCCTTAGGCTCCCTTTTTCTATTATAAAGTAATTTATACTTCATTGTTCTTTTTCTCATTCTTTTTGTACATTATTATTAGGGATAGTTGCAAATATAGCAATTAGATTCAAAATATTAGTATATATAGCAACATTTTTTTTAAATTACAAATATAACAAAATCTGTCAAAGTTCATCAATGATAGAAGTCTATCGTTGATAGACCATGTTGCAAATATTGGTCTATCACTAGTAGACCATAAGTGTCTATCGGCGGTAGAAGTCTATCACCTATTGATTTTGCTATATTTGCAATATTTTTAAAATGTTGCTATATACTTAATTATTATTTCTAAAATTGCTTCTTATTGTAATTACCCTTATTATTGTTACTATTTATTTATTTTGGAGGCTTGTAGTTTATTTATTTATTATTGTTATTATTTATTTATTTCATGTCGATAAATCATGTCTGAGTGCACTTCTACACTCATGGCAGAATCTTGGTCTCATTGAAGAGAGCAAAGTCTCTAGATCCTTGCCTTGGAGGCGACCGACAAATGTTTTCCGTGTCAGGGAGGATGTTCGTCCAATCTTCTGGTATTTCTTCTGCTTATTGCAAACCAAGGATGCATTCCTAATGGTTTTTGGGTGCATAAATTTTTTTTACACCAGGTTAATATTGAGATAGAGCTGATCTATTTAGTTGTTGATTACAGGGCTAATCGTCCTCGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTACCCCCATGGTAGATGGAGTGATTCTAGAAATCCTTCATATGGGGCATTGACTGACTATCAGGTATGAATAACGAACTAGAACTTTTAATACATAATCTTTGTGAGTGCCGTTCATTAATACATGCTCTATGTTTATATTAGTTATATGTGTGTGAGAGAGAGAGAGCTGTAGGGAGGAGATTTCCTTTTACATCCGACTTCTTGTTGACCGCCAGAAAACATCTACATTAATTCAGGACTTAAAAATTGTCTTTGATTAGTCTGACACCATAACATAATCACAATATTCTGTTGAGTCGAATGAAGCTTATGATTTTAGGAAAAGATAATTTTCTAGAAAGTCAGTAACAACTCCTGTTGGAAGCTTTTGGTAGACTTTTGCCCCCAAATAACGTGTTTGTTTTTAGTGTTATTATTGCCAAGCTGGTTAGATGATTTGTTAGAAGTTTAATCAGAGTTATTTTTGCTTTGTTTTCCACTTAAGTTTATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGGTACTGTTCCTAACCAAGAGTGTAGAGAAATTTTGTTACAACTGCTAGCGATCAACTAGTAATGTTTAGACTTTTTTGTATCAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGTAAATACAAGTTAATGCATTTGTACAATTAACTTTTTTGACAGCCCAACTGACCAAGTGTTTGAAAAAGTAGGAAAAGTACTTCAAAACAAAAACTGAACTAGATATTAATAAAAATGAAAGACAATATATTATTTGCCAAGTTACATATTGGAGGGAGTAAATTTAATATCCCCAAGGGAATGAAGCCCACTTCAGTTTCTATTCATATTCTTCTTGAAATCAGAAACCTGTACAGGGCCCATCTGTCAAAAGACAGTTAATGGTCATTTGAAGGGATGAAACACGTGTAGATGGTATGTAAACTTTAACCACGAATATAACATCTTAACAATACAATCATCATGCATGAGTCGCCCTTGTGTAGTGTAGGGGAATTATTATTTTCTTGCTGGAAAATTCAACACTTGGTCTCTACTCTTTTTGTAGTGATCTCATTAGGTTCTAACTTCTGAAATCTGTGCTAAATGGTGGCAAAATGTTTAGTAATCTGACCAATGTTGGGACGATTGATAATGATTTACAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAGTTGCTTGATGAGGTTGGTACACTTGCTATATCTTTTCAGTGCTCAATTAATTTCGGTTTAATATTTCCTAAAAAATAACATCCAAATGTGTGATTGAAAGGCTTAATTAGTAAAAAGATTAAATGTTGTTTTATCTACAAGGTTATTAATATATATAATGATAGAAGATGAGAAGGTATGTGGAGGATTATTATTGATGACGAAGCTATGTTATTTGTTTCAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGAGATGAGCTCTGCTATTCCTTTCCATGATTCTAGCTTTTTCTTCCATCTTTCGATTTCTCATTGAAGCTTGGATAAGGTTAATTCAAGAAGAATTTAATTTTATTTCCTTTTTATCTTTTTTTTTTCCCTACATTTTCCGTTTGTTTGAGTTTATAATATAATTTCCCAAAGGGGAGGGGTGTCCATTTAAATACATTAGAAACAATTTTGGGATTTTAAACTTCCCGAGGTTGGATGTAATGTATAATTCATTTGTGACTGAAGTGTATTAGATTATAAATATCTCTAAAGGTGGTCAAACCTTGTGCCTGGGATACTACATTTATATTTTATGGTTCTTTTAAAGTTTTAAAATCAGAC

mRNA sequence

CTAACCGACAATGCTCAGATGCGATTTCCTATCGTCATTCCCCTTCTCTTTCACTCATTTCTCTCTTTCCATCTCTCATTCTCCTCTTTTTTTTTTTCTCTTTCGTAAGTCTTCACTTTCGATTCTATCCTTCTTCTACTTTCCCCCATATGTTTCACATTGTTCATTATTTCCATTGATTTCCAGGTTGCTGCTTCCGATCTCTAACTCCTACACCACTCAAGGGAAGAGAAGATGAAGGTGATTGACAAGATCCGGGCAGCCACCACCGGCGATGGCGGAAGAGTTGTTTTCTCCTTTGAGTACTTCCCTCCCAAGACAGAGGATGGTGTCGATAATCTTTTTGAGAGGATGGACCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCTGGTGGCTCCACTGCCGATCTCACCCTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTTGAGACCATGATGCATCTCACTTGCACCAATATGCCTGTTGAGAAGATCGACCACGCTCTTCAGACTATCAAGGCCAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGTGACCCTCCCCATGGACAGGACAAATTTGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACATATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCTCATCCGGATGTGATTGGAAGCAATGGCGTGGCGACGACTGAAGGCTACCAAAGCGATCTGGCTTATCTTAAGAAAAAGTTCGATGCTGGGGCTGATCTGATTGTTACTCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGTCAAATTGGAATTACTTGCCCAATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACTAAGATTCCAGACGAGATCATGGCTGCCTTAGAGCCTATCAAGGATAATGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGTAAGAAGATTTTGGCTCATGGAATCAAGACTCTGCATATTTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTCATTGAAGAGAGCAAAGTCTCTAGATCCTTGCCTTGGAGGCGACCGACAAATGTTTTCCGTGTCAGGGAGGATGTTCGTCCAATCTTCTGGGCTAATCGTCCTCGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTACCCCCATGGTAGATGGAGTGATTCTAGAAATCCTTCATATGGGGCATTGACTGACTATCAGTTTATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAGTTGCTTGATGAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGAGATGAGCTCTGCTATTCCTTTCCATGATTCTAGCTTTTTCTTCCATCTTTCGATTTCTCATTGAAGCTTGGATAAGGTTAATTCAAGAAGAATTTAATTTTATTTCCTTTTTATCTTTTTTTTTTCCCTACATTTTCCGTTTGTTTGAGTTTATAATATAATTTCCCAAAGGGGAGGGGTGTCCATTTAAATACATTAGAAACAATTTTGGGATTTTAAACTTCCCGAGGTTGGATGTAATGTATAATTCATTTGTGACTGAAGTGTATTAGATTATAAATATCTCTAAAGGTGGTCAAACCTTGTGCCTGGGATACTACATTTATATTTTATGGTTCTTTTAAAGTTTTAAAATCAGAC

Coding sequence (CDS)

ATGAAGGTGATTGACAAGATCCGGGCAGCCACCACCGGCGATGGCGGAAGAGTTGTTTTCTCCTTTGAGTACTTCCCTCCCAAGACAGAGGATGGTGTCGATAATCTTTTTGAGAGGATGGACCGTATGGTGGCTCATAATCCTTCCTTCTGCGACATTACTTGGGGCGCTGGTGGCTCCACTGCCGATCTCACCCTCGACATTGCCAACAAGATGCAGAACATGATCTGTGTTGAGACCATGATGCATCTCACTTGCACCAATATGCCTGTTGAGAAGATCGACCACGCTCTTCAGACTATCAAGGCCAATGGCCTTCAGAATGTCCTCGCGCTTCGCGGTGACCCTCCCCATGGACAGGACAAATTTGTCCAGATCGAAGGCGGCTTCGCTTGTGCTCTCGATCTTGTTAAACATATCAGGGAGAAATATGGTGACTACTTCGGCATTACTGTAGCTGGATATCCAGAGGCTCATCCGGATGTGATTGGAAGCAATGGCGTGGCGACGACTGAAGGCTACCAAAGCGATCTGGCTTATCTTAAGAAAAAGTTCGATGCTGGGGCTGATCTGATTGTTACTCAACTCTTCTATGACACTGACATTTTCCTCAAGTTTGTTAATGATTGTCGTCAAATTGGAATTACTTGCCCAATTGTACCTGGTATTATGCCCATCAATAATTACAAAGGTTTCTTACGCATGACTGGCTTTTGCAAAACTAAGATTCCAGACGAGATCATGGCTGCCTTAGAGCCTATCAAGGATAATGAAGAAGCTGTCAAAGCTTATGGAATTCACCTTGGAACTGAGATGTGTAAGAAGATTTTGGCTCATGGAATCAAGACTCTGCATATTTATACACTGAATATGGAGAAATCGGCATTGGCTATACTAATGAATCTTGGTCTCATTGAAGAGAGCAAAGTCTCTAGATCCTTGCCTTGGAGGCGACCGACAAATGTTTTCCGTGTCAGGGAGGATGTTCGTCCAATCTTCTGGGCTAATCGTCCTCGAAGCTACATATCAAGGACCATAGGTTGGGACCAGTACCCCCATGGTAGATGGAGTGATTCTAGAAATCCTTCATATGGGGCATTGACTGACTATCAGTTTATGCGGCCACGGGCACGTGACAAGAAGCTTCTTGAGGAATGGGCACTCCCATTGAAAAGCATTGAAGATGTTTATGAGAAATTTATGAAGTACTGCCTTGGAAAGTTGAGAAGCAGTCCGTGGTCTGAATTAGAAGGTCTTCAGGCTGAGACAAGGATGATAAGTGAACAGCTTGGGAAAATTAACATGAAAGGTTTTCTTTCTATCAACAGCCAACCAGCAGTTAATGGAGAAAGATCTGATTCTCCTTCCGTAGGATGGGGTGGGCCAGGAGGTTACGTTTATCAAAAGGCGTACATTGAGTTTTTCTGTTCCAGGGAAAAGTTGGATGCTATTGTTGACAAATGCAGGGCCCTTCCATCTCTCACCTACATGGCGGTGAATAAGGAAGGCATTTGGGTATCCAACGTCACTCAAAAGGATGTGAATGCTGTAACATGGGGAGTTTTCCCAGCCAAAGAGATCATCCAGCCAACCGTTGTCGATCCTGCCAGTTTTCTTGTGTGGAAAGATGAGGCGTTTGAGATTTGGTCAAGGGGATGGGCTGCCTTGTATCCTGAGGGCGACTGGTCCCGAAAGTTGCTTGATGAGATACAGAGCAGCTACTACTTGGTGAGTCTGGTGGATAACGATTATATCAATTCCGACCTCTTTGCTATTTTGGAGGACTTCTGA

Protein sequence

MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGSTADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQDKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILMNLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSRNPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQAETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREKLDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLVWKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Homology
BLAST of IVF0006951 vs. ExPASy Swiss-Prot
Match: O80585 (Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 PE=1 SV=2)

HSP 1 Score: 1043.5 bits (2697), Expect = 9.2e-304
Identity = 481/595 (80.84%), Postives = 543/595 (91.26%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI+  +  D G+  FSFE+FPPKTEDGVDNLFERMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQ--SLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMTGFCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+MAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +P GRW DSR
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSEL+GLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ETR+I+EQL K+N KGFL+INSQP+VN ERSDSP+VGWGGP GYVYQKAY+EFFCS+EK
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+V+KC+ALPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 596
           WKDEAFE WSR WA LYPE D SR LL+E+++SYYLVSLV+NDYIN D+FA+  D
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

BLAST of IVF0006951 vs. ExPASy Swiss-Prot
Match: Q75HE6 (Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0815200 PE=2 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 1.0e-302
Identity = 486/595 (81.68%), Postives = 537/595 (90.25%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI+ A     GR VFSFEYFPPKTE+G+DNLFERMDRMVAH P+FCDITWGAGGS
Sbjct: 1   MKVIEKIQEAAA--DGRTVFSFEYFPPKTEEGLDNLFERMDRMVAHGPNFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKID AL TIK+NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDDALTTIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+ GGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I S   AT E Y +DLAY
Sbjct: 121 DKFVQVAGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQSTEGATPEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LK+K DAGADLI+TQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKQKVDAGADLIITQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP EI AALEPIKDNEEAVKAYGIHLGTEMCKKILA GIKTLH+YTLNMEKSAL ILM
Sbjct: 241 TKIPAEITAALEPIKDNEEAVKAYGIHLGTEMCKKILATGIKTLHLYTLNMEKSALGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESK+SRSLPWR PTNVFRV+EDVRPIFWANRP+SYISRT+GWDQYPHGRW DSR
Sbjct: 301 NLGLIEESKISRSLPWRPPTNVFRVKEDVRPIFWANRPKSYISRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQF RPR R KKL EEWA+P+KS+ED+ E+FM +C GKL SSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFTRPRGRGKKLQEEWAVPVKSVEDINERFMNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ET++I +QL KIN KGFL+INSQPAVNGERSDS SVGWGGPGGYVYQKAY+EFFCS+EK
Sbjct: 421 PETKIIDDQLVKINQKGFLTINSQPAVNGERSDSTSVGWGGPGGYVYQKAYLEFFCSKEK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LD +++K +A PSLTY+AVNK+G   SN+    VNAVTWGVFP KEI+QPTVVD ASF+V
Sbjct: 481 LDQLIEKSKAFPSLTYIAVNKDGESFSNIPTNAVNAVTWGVFPGKEIVQPTVVDSASFMV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 596
           WKDEAFEIWS+GWA L+PEGD SR++LD++Q SY+LVSLVDNDYIN DLFA  ++
Sbjct: 541 WKDEAFEIWSKGWACLFPEGDSSREILDKVQKSYFLVSLVDNDYINGDLFAAFKE 593

BLAST of IVF0006951 vs. ExPASy Swiss-Prot
Match: Q9SE94 (Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 2.6e-298
Identity = 476/591 (80.54%), Postives = 532/591 (90.02%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KI  A  GD GR  FSFEYFPPKTE+GV+NLFERMDRMVAH PSFCDITWGAGGS
Sbjct: 1   MKVIEKILEA-AGD-GRTAFSFEYFPPKTEEGVENLFERMDRMVAHGPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IAN+MQNM+CVETMMHLTCTNMPVEKIDHAL+TIK+NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIANRMQNMVCVETMMHLTCTNMPVEKIDHALETIKSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV+HIR KYGDYFGITVAGYPEAHPD I   G AT E Y +DLAY
Sbjct: 121 DKFVQVEGGFACALDLVQHIRAKYGDYFGITVAGYPEAHPDAIQGEGGATLEAYSNDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LK+K DAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKRKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP EI AAL+PIKDNEEAV+ YGIHLGTEMCKKILA GIKTLH+YTLNM+KSA+ ILM
Sbjct: 241 TKIPSEITAALDPIKDNEEAVRQYGIHLGTEMCKKILATGIKTLHLYTLNMDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSR LPWR  TNVFRV+EDVRPIFWANRP+SY+ RT+GWDQYPHGRW DSR
Sbjct: 301 NLGLIEESKVSRPLPWRPATNVFRVKEDVRPIFWANRPKSYLKRTLGWDQYPHGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTD+QF RPR R KKL EEWA+PLKS+ED+ E+F  +C GKL SSPWSEL+GLQ
Sbjct: 361 NPSYGALTDHQFTRPRGRGKKLQEEWAVPLKSVEDISERFTNFCQGKLTSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ET++I +QL  IN KGFL+INSQPAVNGE+SDSP+VGWGGPGGYVYQKAY+EFFC++EK
Sbjct: 421 PETKIIDDQLVNINQKGFLTINSQPAVNGEKSDSPTVGWGGPGGYVYQKAYLEFFCAKEK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LD +++K +A PSLTY+AVNK+G   SN++   VNAVTWGVFP KEIIQPTVVD ASF+V
Sbjct: 481 LDQLIEKIKAFPSLTYIAVNKDGETFSNISPNAVNAVTWGVFPGKEIIQPTVVDHASFMV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFA 592
           WKDEAFEIW+RGW  ++PEGD SR+LL+++Q +YYLVSLVDNDY+  DLFA
Sbjct: 541 WKDEAFEIWTRGWGCMFPEGDSSRELLEKVQKTYYLVSLVDNDYVQGDLFA 589

BLAST of IVF0006951 vs. ExPASy Swiss-Prot
Match: Q9SE60 (Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 PE=1 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 3.9e-294
Identity = 460/592 (77.70%), Postives = 534/592 (90.20%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  + G+  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSEL+GLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ET++I+EQLGKIN  GFL+INSQP+VN  +SDSP++GWGGPGGYVYQKAY+EFFCS++K
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LD +V+K +A PS+TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAI 593
           WKDEAFEIWSR WA LYPE D SRKLL+E+++SYYLVSLVDN+YIN D+F++
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSV 590

BLAST of IVF0006951 vs. ExPASy Swiss-Prot
Match: P42898 (Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3)

HSP 1 Score: 527.3 bits (1357), Expect = 2.2e-148
Identity = 266/598 (44.48%), Postives = 377/598 (63.04%), Query Frame = 0

Query: 14  DGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGSTAD----LTLDIA 73
           + G   FS E+FPP+T +G  NL  R DRM A  P + D+TW   G         ++ IA
Sbjct: 54  ESGDKWFSLEFFPPRTAEGAVNLISRFDRMAAGGPLYIDVTWHPAGDPGSDKETSSMMIA 113

Query: 74  NKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQDKFVQIEGG 133
           +   N   +ET++H+TC    +E+I   L   K  GL+N++ALRGDP    D++ + EGG
Sbjct: 114 STAVNYCGLETILHMTCCRQRLEEITGHLHKAKQLGLKNIMALRGDPI--GDQWEEEEGG 173

Query: 134 FACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAYLKKKFDAGA 193
           F  A+DLVKHIR ++GDYF I VAGYP+ HP+  GS        +++DL +LK+K  AGA
Sbjct: 174 FNYAVDLVKHIRSEFGDYFDICVAGYPKGHPEA-GS--------FEADLKHLKEKVSAGA 233

Query: 194 DLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCKTKIPDEIMA 253
           D I+TQLF++ D F +FV  C  +GITCPIVPGI PI  Y    ++    K ++P EI  
Sbjct: 234 DFIITQLFFEADTFFRFVKACTDMGITCPIVPGIFPIQGYHSLRQLVKLSKLEVPQEIKD 293

Query: 254 ALEPIKDNEEAVKAYGIHLGTEMCKKILAHG-IKTLHIYTLNMEKSALAILMNLGLIEES 313
            +EPIKDN+ A++ YGI L   +C+++LA G +  LH YTLN E +   +L  LG+  E 
Sbjct: 294 VIEPIKDNDAAIRNYGIELAVSLCQELLASGLVPGLHFYTLNREMATTEVLKRLGMWTED 353

Query: 314 KVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSRNPSYGALT 373
              R LPW    +  R  EDVRPIFWA+RP+SYI RT  WD++P+GRW +S +P++G L 
Sbjct: 354 P-RRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELK 413

Query: 374 DYQ--FMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLG-------KLRSSPWSELEGL 433
           DY   +++ ++  ++LL+ W   L S E V+E F+ Y  G       K+   PW++ E L
Sbjct: 414 DYYLFYLKSKSPKEELLKMWGEELTSEESVFEVFVLYLSGEPNRNGHKVTCLPWND-EPL 473

Query: 434 QAETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSRE 493
            AET ++ E+L ++N +G L+INSQP +NG+ S  P VGWG  GGYV+QKAY+EFF SRE
Sbjct: 474 AAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRE 533

Query: 494 KLDAIVDKCRALP-SLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASF 553
             +A++   +     + Y  VN +G  ++N  +   NAVTWG+FP +EIIQPTVVDP SF
Sbjct: 534 TAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSF 593

Query: 554 LVWKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDY-INSDLFAILED 596
           + WKDEAF +W   W  LY E   SR ++  I  +Y+LV+LVDND+ +++ L+ ++ED
Sbjct: 594 MFWKDEAFALWIERWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVED 638

BLAST of IVF0006951 vs. ExPASy TrEMBL
Match: A0A5A7V2U8 (Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00900 PE=3 SV=1)

HSP 1 Score: 1241.9 bits (3212), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK
Sbjct: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV
Sbjct: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 597
           WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

BLAST of IVF0006951 vs. ExPASy TrEMBL
Match: A0A0A0LIC6 (Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 PE=3 SV=1)

HSP 1 Score: 1233.0 bits (3189), Expect = 0.0e+00
Identity = 590/596 (98.99%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAAT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLHIYTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETRMISEQLGKINMKGFLSINSQPAVNGERSDSP VGWGGPGGYVYQKAYIEFFCSREK
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDAIVDKCRALPSLTYMAVNKEGIWVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF+V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 597
           WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of IVF0006951 vs. ExPASy TrEMBL
Match: A0A6J1DPD3 (Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC111022964 PE=3 SV=1)

HSP 1 Score: 1203.0 bits (3111), Expect = 0.0e+00
Identity = 570/596 (95.64%), Postives = 588/596 (98.66%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KIRAAT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIEKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHVYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKV+RSLPWRRPTNVFR+REDVRPIFWANRP+SYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVARSLPWRRPTNVFRIREDVRPIFWANRPKSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAY+EFFCSRE 
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYLEFFCSREN 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+VDKCR LP+LTYMAVNKEGIWVSN+TQKDVNAVTWGVFPAKEIIQPTVVDPASF V
Sbjct: 481 LDALVDKCRVLPNLTYMAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 597
           WKDEAFEIW RGWA+LYPEGD SRKLL+E+QSSYYLVSLVDNDY++S+LFAILEDF
Sbjct: 541 WKDEAFEIWLRGWASLYPEGDLSRKLLEEVQSSYYLVSLVDNDYVHSNLFAILEDF 596

BLAST of IVF0006951 vs. ExPASy TrEMBL
Match: A0A6J1I4Y4 (Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 PE=3 SV=1)

HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 570/596 (95.64%), Postives = 583/596 (97.82%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI  AT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITEATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN+TQ DVNAVTWGVFPAKEI+QPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDNDY+NS+LFA LE F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

BLAST of IVF0006951 vs. ExPASy TrEMBL
Match: A0A6J1EQ45 (Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC111435611 PE=3 SV=1)

HSP 1 Score: 1194.5 bits (3089), Expect = 0.0e+00
Identity = 567/596 (95.13%), Postives = 583/596 (97.82%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI AAT GDGGRVVFSFEYFPPKTEDGVDNLF+RMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITAATAGDGGRVVFSFEYFPPKTEDGVDNLFDRMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATAEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGE+SDSPSVGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGEKSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN+TQ DVNAVTWGVFPAKEI+QPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 597
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDN+Y+NS+LFA LE F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDPSRKFLEEIQSSYYLVSLVDNNYVNSNLFAALEGF 596

BLAST of IVF0006951 vs. NCBI nr
Match: KAA0060205.1 (methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1229 bits (3179), Expect = 0.0
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK
Sbjct: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV
Sbjct: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596
           WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596

BLAST of IVF0006951 vs. NCBI nr
Match: XP_004138970.2 (methylenetetrahydrofolate reductase 2 [Cucumis sativus] >KGN61503.1 hypothetical protein Csa_006097 [Cucumis sativus])

HSP 1 Score: 1220 bits (3156), Expect = 0.0
Identity = 590/596 (98.99%), Postives = 592/596 (99.33%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIRAAT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 43  MKVIDKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 102

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 103 TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 162

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 163 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 222

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 223 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 282

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKIL HGIKTLHIYTLNMEKSALAILM
Sbjct: 283 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILTHGIKTLHIYTLNMEKSALAILM 342

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR
Sbjct: 343 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 402

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ
Sbjct: 403 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 462

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETRMISEQLGKINMKGFLSINSQPAVNGERSDSP VGWGGPGGYVYQKAYIEFFCSREK
Sbjct: 463 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPCVGWGGPGGYVYQKAYIEFFCSREK 522

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDAIVDKCRALPSLTYMAVNKEGIWVSN TQKDVNAVTWGVFPAKEIIQPTVVDPASF+V
Sbjct: 523 LDAIVDKCRALPSLTYMAVNKEGIWVSNATQKDVNAVTWGVFPAKEIIQPTVVDPASFIV 582

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596
           WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF
Sbjct: 583 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 638

BLAST of IVF0006951 vs. NCBI nr
Match: XP_038874836.1 (methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 methylenetetrahydrofolate reductase 2-like [Benincasa hispida])

HSP 1 Score: 1203 bits (3112), Expect = 0.0
Identity = 579/596 (97.15%), Postives = 590/596 (98.99%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKIR ATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKIREATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESK+SRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKISRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKS+EDVYEKFMKYCLGKLRSSPWSELEGLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSVEDVYEKFMKYCLGKLRSSPWSELEGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGK+NMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK
Sbjct: 421 AETRIISEQLGKLNMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LD +VDKCR+LPSLTYMAVNKEGIWVSN+TQKDVNAVTWGVFPAKEIIQPTVVDPASFLV
Sbjct: 481 LDVVVDKCRSLPSLTYMAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596
           WKDEAFEIWSRGWAALYPEGD SRKLL+EIQSSYYLVSLVDNDY+NS+LFA LEDF
Sbjct: 541 WKDEAFEIWSRGWAALYPEGDPSRKLLEEIQSSYYLVSLVDNDYVNSNLFAALEDF 596

BLAST of IVF0006951 vs. NCBI nr
Match: XP_022155988.1 (methylenetetrahydrofolate reductase 2-like [Momordica charantia])

HSP 1 Score: 1190 bits (3078), Expect = 0.0
Identity = 570/596 (95.64%), Postives = 588/596 (98.66%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVI+KIRAAT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIEKIRAATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVAT EGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATPEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHVYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKV+RSLPWRRPTNVFR+REDVRPIFWANRP+SYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVARSLPWRRPTNVFRIREDVRPIFWANRPKSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAY+EFFCSRE 
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYLEFFCSREN 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+VDKCR LP+LTYMAVNKEGIWVSN+TQKDVNAVTWGVFPAKEIIQPTVVDPASF V
Sbjct: 481 LDALVDKCRVLPNLTYMAVNKEGIWVSNITQKDVNAVTWGVFPAKEIIQPTVVDPASFHV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596
           WKDEAFEIW RGWA+LYPEGD SRKLL+E+QSSYYLVSLVDNDY++S+LFAILEDF
Sbjct: 541 WKDEAFEIWLRGWASLYPEGDLSRKLLEEVQSSYYLVSLVDNDYVHSNLFAILEDF 596

BLAST of IVF0006951 vs. NCBI nr
Match: XP_022970234.1 (probable methylenetetrahydrofolate reductase [Cucurbita maxima])

HSP 1 Score: 1186 bits (3069), Expect = 0.0
Identity = 570/596 (95.64%), Postives = 583/596 (97.82%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI  AT GDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS
Sbjct: 1   MKVIDKITEATAGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIK+NGLQNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKSNGLQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY
Sbjct: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMT FCK
Sbjct: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTSFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIPDE+MAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLH+YTLNMEKSALAILM
Sbjct: 241 TKIPDEVMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHMYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLIEESKVSRSLPWRRPTNVFR+REDVRPIFWANRPRSYISRTIGWDQYPHGRWSDS 
Sbjct: 301 NLGLIEESKVSRSLPWRRPTNVFRIREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSS 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSEL+GLQ
Sbjct: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
           AETR+ISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGG GGYVYQKAYIEFFCSRE 
Sbjct: 421 AETRIISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGSGGYVYQKAYIEFFCSREN 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+VDKCR LPSLTYMAVNKEGIWVSN+TQ DVNAVTWGVFPAKEI+QPTVVDPASFLV
Sbjct: 481 LDAVVDKCRGLPSLTYMAVNKEGIWVSNITQNDVNAVTWGVFPAKEIVQPTVVDPASFLV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILEDF 596
           WKDEAFEIWSRGWA+LYPEGD SRK L+EIQSSYYLVSLVDNDY+NS+LFA LE F
Sbjct: 541 WKDEAFEIWSRGWASLYPEGDLSRKFLEEIQSSYYLVSLVDNDYVNSNLFAALEGF 596

BLAST of IVF0006951 vs. TAIR 10
Match: AT2G44160.1 (methylenetetrahydrofolate reductase 2 )

HSP 1 Score: 1043.5 bits (2697), Expect = 6.5e-305
Identity = 481/595 (80.84%), Postives = 543/595 (91.26%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKVIDKI+  +  D G+  FSFE+FPPKTEDGVDNLFERMDRMVA+ P+FCDITWGAGGS
Sbjct: 1   MKVIDKIQ--SLADEGKTAFSFEFFPPKTEDGVDNLFERMDRMVAYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTLDIA++MQN++CVE+MMHLTCTNMPVEKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLDIASRMQNVVCVESMMHLTCTNMPVEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGF CALDLV HIR KYGDYFGITVAGYPEAHPDVIG NG+A+ E YQSDL Y
Sbjct: 121 DKFVQVEGGFDCALDLVNHIRSKYGDYFGITVAGYPEAHPDVIGENGLASNEAYQSDLEY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI+CPIVPGIMPINNY+GFLRMTGFCK
Sbjct: 181 LKKKIDAGADLIVTQLFYDTDIFLKFVNDCRQIGISCPIVPGIMPINNYRGFLRMTGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+MAALEPIKDNEEAVKAYGIHLGTEMCKK+LAHG+K+LH+YTLNMEKSALAILM
Sbjct: 241 TKIPVEVMAALEPIKDNEEAVKAYGIHLGTEMCKKMLAHGVKSLHLYTLNMEKSALAILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLG+I+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +P GRW DSR
Sbjct: 301 NLGMIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWEDFPQGRWGDSR 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           + SYGAL+D+QF RPRARDKKL +EW +PLKS+ED+ EKF + CLG L+SSPWSEL+GLQ
Sbjct: 361 SASYGALSDHQFSRPRARDKKLQQEWVVPLKSVEDIQEKFKELCLGNLKSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ETR+I+EQL K+N KGFL+INSQP+VN ERSDSP+VGWGGP GYVYQKAY+EFFCS+EK
Sbjct: 421 PETRIINEQLIKVNSKGFLTINSQPSVNAERSDSPTVGWGGPVGYVYQKAYLEFFCSKEK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LDA+V+KC+ALPS+TYMAVNK   WVSN  Q DVNAVTWGVFPAKEIIQPT+VDPASF V
Sbjct: 481 LDAVVEKCKALPSITYMAVNKGEQWVSNTAQADVNAVTWGVFPAKEIIQPTIVDPASFNV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAILED 596
           WKDEAFE WSR WA LYPE D SR LL+E+++SYYLVSLV+NDYIN D+FA+  D
Sbjct: 541 WKDEAFETWSRSWANLYPEADPSRNLLEEVKNSYYLVSLVENDYINGDIFAVFAD 593

BLAST of IVF0006951 vs. TAIR 10
Match: AT3G59970.3 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 1011.5 bits (2614), Expect = 2.7e-295
Identity = 460/592 (77.70%), Postives = 534/592 (90.20%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  + G+  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEKFMKYCLGKLRSSPWSELEGLQ 420
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV EKF + C+G L+SSPWSEL+GLQ
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQEKFKELCIGNLKSSPWSELDGLQ 420

Query: 421 AETRMISEQLGKINMKGFLSINSQPAVNGERSDSPSVGWGGPGGYVYQKAYIEFFCSREK 480
            ET++I+EQLGKIN  GFL+INSQP+VN  +SDSP++GWGGPGGYVYQKAY+EFFCS++K
Sbjct: 421 PETKIINEQLGKINSNGFLTINSQPSVNAAKSDSPAIGWGGPGGYVYQKAYLEFFCSKDK 480

Query: 481 LDAIVDKCRALPSLTYMAVNKEGIWVSNVTQKDVNAVTWGVFPAKEIIQPTVVDPASFLV 540
           LD +V+K +A PS+TYMAVNK   WVSN  + DVNAVTWGVFPAKE+IQPT+VDPASF V
Sbjct: 481 LDTLVEKSKAFPSITYMAVNKSENWVSNTGESDVNAVTWGVFPAKEVIQPTIVDPASFKV 540

Query: 541 WKDEAFEIWSRGWAALYPEGDWSRKLLDEIQSSYYLVSLVDNDYINSDLFAI 593
           WKDEAFEIWSR WA LYPE D SRKLL+E+++SYYLVSLVDN+YIN D+F++
Sbjct: 541 WKDEAFEIWSRSWANLYPEDDPSRKLLEEVKNSYYLVSLVDNNYINGDIFSV 590

BLAST of IVF0006951 vs. TAIR 10
Match: AT3G59970.2 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 699.1 bits (1803), Expect = 3.0e-201
Identity = 319/399 (79.95%), Postives = 364/399 (91.23%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  + G+  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYEK 400
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV E+
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQER 397

BLAST of IVF0006951 vs. TAIR 10
Match: AT3G59970.1 (methylenetetrahydrofolate reductase 1 )

HSP 1 Score: 698.4 bits (1801), Expect = 5.1e-201
Identity = 319/398 (80.15%), Postives = 363/398 (91.21%), Query Frame = 0

Query: 1   MKVIDKIRAATTGDGGRVVFSFEYFPPKTEDGVDNLFERMDRMVAHNPSFCDITWGAGGS 60
           MKV+DKI++ T  + G+  FSFE+FPPKTEDGV+NLFERMDR+V++ P+FCDITWGAGGS
Sbjct: 1   MKVVDKIKSVT--EQGQTAFSFEFFPPKTEDGVENLFERMDRLVSYGPTFCDITWGAGGS 60

Query: 61  TADLTLDIANKMQNMICVETMMHLTCTNMPVEKIDHALQTIKANGLQNVLALRGDPPHGQ 120
           TADLTL+IA++MQN+ICVETMMHLTCTNMP+EKIDHAL+TI++NG+QNVLALRGDPPHGQ
Sbjct: 61  TADLTLEIASRMQNVICVETMMHLTCTNMPIEKIDHALETIRSNGIQNVLALRGDPPHGQ 120

Query: 121 DKFVQIEGGFACALDLVKHIREKYGDYFGITVAGYPEAHPDVIGSNGVATTEGYQSDLAY 180
           DKFVQ+EGGFACALDLV HIR KYGDYFGITVAGYPEAHPDVI ++G+AT E YQSDLAY
Sbjct: 121 DKFVQVEGGFACALDLVNHIRSKYGDYFGITVAGYPEAHPDVIEADGLATPESYQSDLAY 180

Query: 181 LKKKFDAGADLIVTQLFYDTDIFLKFVNDCRQIGITCPIVPGIMPINNYKGFLRMTGFCK 240
           LKKK DAGADLIVTQLFYDTDIFLKFVNDCRQIGI CPIVPGIMPI+NYKGFLRM GFCK
Sbjct: 181 LKKKVDAGADLIVTQLFYDTDIFLKFVNDCRQIGINCPIVPGIMPISNYKGFLRMAGFCK 240

Query: 241 TKIPDEIMAALEPIKDNEEAVKAYGIHLGTEMCKKILAHGIKTLHIYTLNMEKSALAILM 300
           TKIP E+ AALEPIKDN+EAVKAYGIH  TEMCKKILAHGI +LH+YTLN++KSA+ ILM
Sbjct: 241 TKIPAELTAALEPIKDNDEAVKAYGIHFATEMCKKILAHGITSLHLYTLNVDKSAIGILM 300

Query: 301 NLGLIEESKVSRSLPWRRPTNVFRVREDVRPIFWANRPRSYISRTIGWDQYPHGRWSDSR 360
           NLGLI+ESK+SRSLPWRRP NVFR +EDVRPIFWANRP+SYISRT GW+ +PHGRW DS 
Sbjct: 301 NLGLIDESKISRSLPWRRPANVFRTKEDVRPIFWANRPKSYISRTKGWNDFPHGRWGDSH 360

Query: 361 NPSYGALTDYQFMRPRARDKKLLEEWALPLKSIEDVYE 399
           + +Y  L+DYQF RP+ RDKKL +EW +PLKSIEDV E
Sbjct: 361 SAAYSTLSDYQFARPKGRDKKLQQEWVVPLKSIEDVQE 396

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O805859.2e-30480.84Methylenetetrahydrofolate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=MTHFR2 ... [more]
Q75HE61.0e-30281.68Probable methylenetetrahydrofolate reductase OS=Oryza sativa subsp. japonica OX=... [more]
Q9SE942.6e-29880.54Methylenetetrahydrofolate reductase 1 OS=Zea mays OX=4577 PE=2 SV=1[more]
Q9SE603.9e-29477.70Methylenetetrahydrofolate reductase 1 OS=Arabidopsis thaliana OX=3702 GN=MTHFR1 ... [more]
P428982.2e-14844.48Methylenetetrahydrofolate reductase OS=Homo sapiens OX=9606 GN=MTHFR PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A5A7V2U80.0e+00100.00Methylenetetrahydrofolate reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A0A0LIC60.0e+0098.99Methylenetetrahydrofolate reductase OS=Cucumis sativus OX=3659 GN=Csa_2G149470 P... [more]
A0A6J1DPD30.0e+0095.64Methylenetetrahydrofolate reductase OS=Momordica charantia OX=3673 GN=LOC1110229... [more]
A0A6J1I4Y40.0e+0095.64Methylenetetrahydrofolate reductase OS=Cucurbita maxima OX=3661 GN=LOC111469179 ... [more]
A0A6J1EQ450.0e+0095.13Methylenetetrahydrofolate reductase OS=Cucurbita moschata OX=3662 GN=LOC11143561... [more]
Match NameE-valueIdentityDescription
KAA0060205.10.0100.00methylenetetrahydrofolate reductase 2 [Cucumis melo var. makuwa] >TYJ95579.1 met... [more]
XP_004138970.20.098.99methylenetetrahydrofolate reductase 2 [Cucumis sativus] >KGN61503.1 hypothetical... [more]
XP_038874836.10.097.15methylenetetrahydrofolate reductase 2-like [Benincasa hispida] >XP_038874837.1 m... [more]
XP_022155988.10.095.64methylenetetrahydrofolate reductase 2-like [Momordica charantia][more]
XP_022970234.10.095.64probable methylenetetrahydrofolate reductase [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G44160.16.5e-30580.84methylenetetrahydrofolate reductase 2 [more]
AT3G59970.32.7e-29577.70methylenetetrahydrofolate reductase 1 [more]
AT3G59970.23.0e-20179.95methylenetetrahydrofolate reductase 1 [more]
AT3G59970.15.1e-20180.15methylenetetrahydrofolate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003171Methylenetetrahydrofolate reductase-likePFAMPF02219MTHFRcoord: 15..303
e-value: 1.7E-123
score: 411.5
IPR003171Methylenetetrahydrofolate reductase-likeCDDcd00537MTHFRcoord: 20..302
e-value: 1.7827E-122
score: 360.778
NoneNo IPR availableGENE3D3.20.20.220coord: 1..306
e-value: 7.6E-120
score: 401.4
NoneNo IPR availablePANTHERPTHR45754:SF4METHYLENETETRAHYDROFOLATE REDUCTASE 1coord: 1..593
NoneNo IPR availablePANTHERPTHR45754METHYLENETETRAHYDROFOLATE REDUCTASEcoord: 1..593
IPR004621Eukaryotic-type methylenetetrahydrofolate reductaseTIGRFAMTIGR00677TIGR00677coord: 19..307
e-value: 1.1E-150
score: 498.6
IPR029041FAD-linked oxidoreductase-likeSUPERFAMILY51730FAD-linked oxidoreductasecoord: 14..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0006951.4IVF0006951.4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009086 methionine biosynthetic process
biological_process GO:0035999 tetrahydrofolate interconversion
biological_process GO:0006555 methionine metabolic process
cellular_component GO:0005829 cytosol
molecular_function GO:0071949 FAD binding
molecular_function GO:0106312 methylenetetrahydrofolate reductase NADH activity
molecular_function GO:0106313 methylenetetrahydrofolate reductase NADPH activity
molecular_function GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity