IVF0006583 (gene) Melon (IVF77) v1

BLAST of IVF0006583 vs. ExPASy Swiss-Prot
Match: Q8L735 (Isoamylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA2 PE=1 SV=2)

HSP 1 Score: 627.9 bits (1618), Expect = 1.3e-178
Identity = 332/691 (48.05%), Postives = 434/691 (62.81%), Query Frame = 0

Query: 89  FGDFTPDGTTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKF--------QWVLIQ 148
           F + + D  T  TET FVK+S  +  + LEFD K +PFYLSF LK         Q +L  
Sbjct: 157 FENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTH 216

Query: 149 A---------------------------ALKLEVIGRLAFLYLLGLVE------------ 208
                                       +       R +   +L L +            
Sbjct: 217 RDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELD 276

Query: 209 ----VIQVRNIWHASFEGVSNFVSYGYQC------------------------------- 268
               V +  ++WHAS +   +FV YGY+C                               
Sbjct: 277 LDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVS 336

Query: 269 --------KVPAFDWDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQ 328
                   K P+FDW  +V PN+P+EKL VYRLNVK FT                 +  +
Sbjct: 337 QKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEK 396

Query: 329 VSFRQILPVHFWLDQEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKEL 388
           VS  + L  +  L  EPIF F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+L
Sbjct: 397 VSHLKTLGTNAVL-LEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKL 456

Query: 389 HANGVEVIVEVVYTHTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSL 448
           H+ G+EV++EVV+THT+  GAL+GIDDSSYY+  R  +L+ ++           + L+SL
Sbjct: 457 HSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESL 516

Query: 449 RYWVTEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELV 508
           RYWVTEFHVDGFCF+NAS LL+G HGE LSRPP VEAIAFDP+L++TKL+AD WDP E++
Sbjct: 517 RYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMM 576

Query: 509 SKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFI 568
            KE RFPHWKRWAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I
Sbjct: 577 PKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYI 636

Query: 569 ARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVP 628
           +RN GL LVD+VSFS   LASELSWNCGEEG TN   VL++RLKQIRNF+F+ ++SLGVP
Sbjct: 637 SRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVP 696

Query: 629 VLNMGDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNF 659
           VLNMGDECG S+ GS     R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+F
Sbjct: 697 VLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDF 756

BLAST of IVF0006583 vs. ExPASy Swiss-Prot
Match: Q6AU80 (Isoamylase 2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA2 PE=1 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 3.6e-147
Identity = 268/566 (47.35%), Postives = 357/566 (63.07%), Query Frame = 0

Query: 167 NIWHASFEGVSNFVSYGYQCK--------------------------------------- 226
           N+WH S   V  +VSY + C                                        
Sbjct: 207 NVWHVSLASVDGYVSYAFCCGGIRRPLLDPYAKVIGDFVSSNSVYDEGVTAPSMRCFASL 266

Query: 227 --VPAFDWDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQIL 286
              P+++W  +  P LP+EKL VYR NV  FT                 ++ +V   + L
Sbjct: 267 AIAPSYNWGRDRHPRLPLEKLVVYRANVALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSL 326

Query: 287 PVHFWLDQEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEV 346
            V+  L  EP+F F + +GPYFP+HFFSP N Y   G S+SAI SMK+MV+ +H NG+EV
Sbjct: 327 GVNAIL-LEPVFPFHQVKGPYFPYHFFSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEV 386

Query: 347 IVEVVYTHTSVDG------ALQGIDDSSYYFANRVANLE----------ERTITLDSLRY 406
           ++EVV+THT+         +++GID+SSYY AN +A  +           + + LDSLR+
Sbjct: 387 LLEVVFTHTAEGESECQTISMRGIDNSSYYIANGIAGCKASILNCNHPVTQKLILDSLRH 446

Query: 407 WVTEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSK 466
           WV +FHVDGFCF+NA FL++G  GE LSRPP +EAI FDP+LS TK++AD W P ++ + 
Sbjct: 447 WVLDFHVDGFCFINAPFLVRGPGGEYLSRPPLLEAITFDPVLSMTKIIADPWSPLDISNV 506

Query: 467 ETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIAR 526
           +  FPHWKRWAEVN++F  D+R F + E LIS LATRLCGSGD+FS  RGPAFSFN ++R
Sbjct: 507 QFPFPHWKRWAEVNTRFSIDVRKFLKREALISDLATRLCGSGDLFST-RGPAFSFNHVSR 566

Query: 527 NVGLPLVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVL 586
           N GL LVDLVSFSN +L SE SWNCGEEGP+    VL+ RL+QIRNF+F+LFVSLGVPVL
Sbjct: 567 NSGLSLVDLVSFSNDDLLSESSWNCGEEGPSENSAVLQTRLRQIRNFLFILFVSLGVPVL 626

Query: 587 NMGDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLK 646
           NMGDECG S+ GSV++ DR   NW  +KT F  + T FI+FL++ RSRR D+FQ R FLK
Sbjct: 627 NMGDECGHSAAGSVSYKDRGPLNWRGMKTTFVKEVTGFISFLTALRSRRGDIFQRREFLK 686

Query: 647 GENIDWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSES 659
            ENI W+ ++   P W+D +  FL + + A+ +E  +       R D+++ FNA+++S S
Sbjct: 687 LENIHWYGSDLCEPGWDDPTSNFLCMHINAEVDEMAA----DSVRGDLYICFNANEESVS 746

BLAST of IVF0006583 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 253.1 bits (645), Expect = 9.0e-66
Identity = 183/562 (32.56%), Postives = 265/562 (47.15%), Query Frame = 0

Query: 170 HASFEGVSNFVS--YG-YQCKVPAFDWDGEVR-PNLPMEKLFVYRLNVKRFT-------- 229
           H+SF   S   +  YG Y  +   FDW  + + PN+P + L +Y +NV+ FT        
Sbjct: 196 HSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMD 255

Query: 230 ---------MTNQVSFRQILPVHFWLDQEPIFQFDEKE--------------GPYFPFHF 289
                       ++   Q L ++  ++  P+F+FDE E                Y   +F
Sbjct: 256 PAIGGSYLGFIEKIPHLQDLGINA-VELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNF 315

Query: 290 FSPTNNYGP-SGASISAINSMKEMVKELHANGVEVIVEVVYTHTS-------VDGALQGI 349
           F+P + Y    G  I A    KEMVK LH+ G+EVI++VVY HT+          + +GI
Sbjct: 316 FAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGI 375

Query: 350 DDSSYYFA---NRVANLE------------ERTITLDSLRYWVTEFHVDGFCFVNASFLL 409
           D+  YY     N++ N                 + LDSLR+WVTE+HVDGF F  AS L 
Sbjct: 376 DNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLC 435

Query: 410 QGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCS 469
           +   G  LS PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  
Sbjct: 436 RATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRD 495

Query: 470 DIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS---- 529
           D+R F +G+ G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++    
Sbjct: 496 DVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHN 555

Query: 530 -------NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDEC 589
                  N       SWNCG EG T    +   R +Q++NF   L +S G P++ MGDE 
Sbjct: 556 EANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEY 615

Query: 590 GQSS-GGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQSRNFLKGENI 649
           G +  G + ++    S N    K     +   F  F    + R    + +  NFL    I
Sbjct: 616 GHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEI 675

Query: 650 DWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALP 659
            W ++N     W+++  KFLA  L      ++ I        DI++AFNA D      +P
Sbjct: 676 TWHEDN-----WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIP 735

BLAST of IVF0006583 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.7e-64
Identity = 179/546 (32.78%), Postives = 261/546 (47.80%), Query Frame = 0

Query: 191 FDWDGEVR-PNLPMEKLFVYRLNVKRFT--------MTNQVSFRQILP--VHFW------ 250
           FDW  + R PNLP   L +Y +NV+ FT         T++ S+  ++    H        
Sbjct: 235 FDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNA 294

Query: 251 LDQEPIFQFDEKEGPYFP--------------FHFFSPTNNYGPSGAS-ISAINSMKEMV 310
           ++  P+F++DE E   +P               +FF+P + Y  +G   ++A   +K+MV
Sbjct: 295 VELLPVFEYDELEFKRYPNPRDHMVNTWGYSTINFFAPMSRYASAGGGPVAASKELKQMV 354

Query: 311 KELHANGVEVIVEVVYTHTS-VDGA------LQGIDDSSYYFANRVANLE---------- 370
           KELH  G+EVI++VVY HT+  D A       +GID+  YY  +   N E          
Sbjct: 355 KELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNT 414

Query: 371 -------ERTITLDSLRYWVTEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPIL 430
                   + + LDSLR+WV E+H+DGF F  AS L +G  G  L  PP ++ IA D +L
Sbjct: 415 LNCNHPVVKELILDSLRHWVEEYHIDGFRFDLASVLCRGPDGCPLDAPPLIKEIAKDAVL 474

Query: 431 SKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCSDIRDFFRGE-GLISSLATRLCGS 490
           S+ K++A+ WD   L     RFP+W RWAE N K+  D+R F +G+ G+    ATR+ GS
Sbjct: 475 SRCKIIAEPWDCGGLY-LVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGS 534

Query: 491 GDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFSNSN-----------LASELSWNCGEEG 550
            D++  + R P    NF+  + G  L DLVS++  +                SWNCG EG
Sbjct: 535 ADLYQVNERKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEG 594

Query: 551 PTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECG-------QSSGGSVAFNDRRSF 610
            TN L VL  R +Q++NF   L +S G P++ MGDE G        S G     N   +F
Sbjct: 595 ETNDLNVLSLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCIN---NF 654

Query: 611 NWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSPPQWEDASCK 661
            W+ L+        +F + +  FR     L + R FL   ++ W ++      WE+   K
Sbjct: 655 QWEQLEQRRDGH-FRFFSEMIKFRHSNPILRRDR-FLNKNDVTWHED-----CWENQESK 714

BLAST of IVF0006583 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 2.1e-59
Identity = 169/550 (30.73%), Postives = 252/550 (45.82%), Query Frame = 0

Query: 191 FDWDGEVRPNLPMEKLFVYRLNVKRFT---------------MTNQVSFRQILPVHFWLD 250
           FDW G++    P + L +Y ++++ FT                 +++ + + L V+  ++
Sbjct: 230 FDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVEHPGTYIGAISKLDYLKELGVN-CVE 289

Query: 251 QEPIFQFDEKE----------GPYFPFHFFSPTNNYGPSG---ASISAINSMKEMVKELH 310
             P  +F+E E            Y   +FFSP   Y   G       AIN  K  V+E H
Sbjct: 290 LMPCHEFNELEYFSCSSKMNFWGYSTINFFSPMIRYSSGGIRNCGRDAINEFKTFVREAH 349

Query: 311 ANGVEVIVEVVYTHTSVDG------ALQGIDDSSYYF------------ANRVANLEE-- 370
             G+EVI++VV+ HT+         + +GID+S+YY                  N     
Sbjct: 350 KRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPKGEFYNYSGCGNTFNCNHPV 409

Query: 371 -RTITLDSLRYWVTEFHVDGFCFVNASFLLQG-------------------HHGELLSRP 430
            R   +D LRYWVTE HVDGF F  AS + +G                     G  L+ P
Sbjct: 410 VREFIVDCLRYWVTEMHVDGFRFDLASIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATP 469

Query: 431 PFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCSDIRDFFRG-EG 490
           P ++ I+ DPIL   KL+A+ WD   L  +  +FPHWK W+E N K+   +R F +G +G
Sbjct: 470 PLIDMISNDPILGDVKLIAEAWDAGGLY-QVGQFPHWKIWSEWNGKYRDIVRQFIKGTDG 529

Query: 491 LISSLATRLCGSGDVF-SDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLAS--------- 550
                A  LCGS  ++ + GR P  S NF+  + G  L DLV+++    +S         
Sbjct: 530 FAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGE 589

Query: 551 --ELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDECGQSSGG--SVA 610
              LSWNCGEEG    L V   R +Q+RNF   L VS GVP+  MGDE G + GG  +  
Sbjct: 590 NHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTY 649

Query: 611 FNDR--RSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLKGENIDWFDNNQSP 656
            +D     F WD  K +  +   +F + ++ FR ++ +     +F   + + W  +    
Sbjct: 650 CHDHYVNYFRWD--KKEESSDLQRFCSLMTKFR-KQCESLGLADFPTAQRLHWHGHQPGK 709

BLAST of IVF0006583 vs. ExPASy TrEMBL
Match: A0A5A7UR47 (Isoamylase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold328G00230 PE=4 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 3.0e-309
Identity = 584/854 (68.38%), Postives = 607/854 (71.08%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT------------------SISL 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT                   +  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  EKWTRLECYICYI--GNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
            K +       Y+  G    ++GQRLGIGGKSKEQRKVATYLFR  +G            
Sbjct: 61  VKSSHRNLSKAYVTSGISVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PDGTTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------- 240
           KFSVELEFDAKHTPFYLSFVLKF   +   + ++    + +F   +G             
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLSI 240

Query: 241 -------------------------------LVE------VIQVRNIWHASFEGVSNFVS 300
                                          L+E      + +  NIWHASFEGVSNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFVS 300

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGYQCK                                                 VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLD 420
           WDGEVRPNLPMEKLFVYRLNVKRFT                 +T ++   + L V+  L 
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT
Sbjct: 421 LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HTSVDGALQGIDDSSYYFANRVANLEER+           + LDSLRYWVTEFHVDGFCF
Sbjct: 481 HTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE
Sbjct: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 659
           VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. ExPASy TrEMBL
Match: A0A1S3BRF8 (isoamylase 2, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492696 PE=4 SV=1)

HSP 1 Score: 1069.7 bits (2765), Expect = 5.0e-309
Identity = 583/854 (68.27%), Postives = 606/854 (70.96%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT------------------SISL 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT                   +  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  EKWTRLECYICYI--GNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
            K +       Y+  G    ++GQRLGIGGKSKEQRKVATYLFR  +G            
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PDGTTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------- 240
           KFSVELEFDAKHTPFYLSFVLKF   +   + ++    + +F   +G             
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 -------------------------------LVE------VIQVRNIWHASFEGVSNFVS 300
                                          L+E      + +  NIWHASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGYQCK                                                 VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLD 420
           WDGEVRPNLPMEKLFVYRLNVKRFT                 +T ++   + L V+  L 
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT
Sbjct: 421 LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HTSVDGALQGIDDSSYYFANRVANLEER+           + LDSLRYWVTEFHVDGFCF
Sbjct: 481 HTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE
Sbjct: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 659
           VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. ExPASy TrEMBL
Match: A0A0A0K551 (Aamy domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G392380 PE=4 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 1.1e-295
Identity = 559/854 (65.46%), Postives = 591/854 (69.20%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKW--------------- 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKT     K                
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  -----TRLECYICYIGNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
                  L       G    ++GQRLGIGGKSKEQR+VATYLFR  +G            
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PD TTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------- 240
           KFSVELEFDAKHTPFYLSFVLK+   +   + ++    + +F   +G             
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 -------------------------------LVE------VIQVRNIWHASFEGVSNFVS 300
                                          L+E      + +  NIWHASFEG S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQC-------------------------------------------------KVPAFD 360
           YGYQC                                                 KVP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTM-----------------TNQVSFRQILPVHFWLD 420
           WDGEV PNLPMEKLFVYRLNV+RFTM                 T ++   + L V+  L 
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYT
Sbjct: 421 LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HTS +GALQGIDDSSYYF NRVANLEE++           + LDSLRYWVTEFHVDGFCF
Sbjct: 481 HTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           VNASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAE
Sbjct: 541 VNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 659
           VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. ExPASy TrEMBL
Match: A0A6J1GPU3 (isoamylase 2, chloroplastic isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 8.2e-264
Identity = 500/854 (58.55%), Postives = 564/854 (66.04%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKWTRLECYICYIGNFGW 60
           MATFLSSL IQPFC +N G TES KLAVSD+ TYGQK +    K              G+
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  --------------------ENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
                               ++GQ+ G GGK++E RKVATYLFR  +G            
Sbjct: 61  TKSLHRNVCRAYAPSRISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    P+ T GATETPFV+TSEG
Sbjct: 121 GFMFSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLGLVE---------- 240
           KF+VELEFDAKH PFYLSF+LK+   +   + +++   + +F   +G             
Sbjct: 181 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 ----------------------------------------VIQVRNIWHASFEGVSNFVS 300
                                                   + +  NIWHASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGY+C+                                                 VPAF 
Sbjct: 301 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFS 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLD 420
           WDGEVRPNLPMEKLF+YRLNVK FT                 +T ++   + L V+  L 
Sbjct: 361 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+T
Sbjct: 421 LEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HT++DGALQGIDDS YYF NRVAN E ++           + LDSLR+WVTEFH+DGFCF
Sbjct: 481 HTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           +NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++ADFWDPQE V KETRFPHWKRWAE
Sbjct: 541 INASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 659
           +NSKF +DI++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 LNSKFSNDIKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. ExPASy TrEMBL
Match: A0A6J1GN63 (isoamylase 2, chloroplastic isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456039 PE=4 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 5.9e-262
Identity = 498/845 (58.93%), Postives = 560/845 (66.27%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKWTRLECYICYIGNFGW 60
           MATFLSSL IQPFC +N G TES KLAVSD+ TYGQK +    K              G+
Sbjct: 1   MATFLSSL-IQPFCGYNRGGTESFKLAVSDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 60

Query: 61  ENG-----------QRLGIGGKSKEQRKVATYLFRRVWG--------------------- 120
                          R+ + GKS+E RKVATYLFR  +G                     
Sbjct: 61  TKSLHRNVCRAYAPSRISV-GKSEEDRKVATYLFRTEFGDLVYAFVGKRGSGFMFSIEIS 120

Query: 121 ----------------------------FGDFTPDGTTGATETPFVKTSEGKFSVELEFD 180
                                       F    P+ T GATETPFV+TSEGKF+VELEFD
Sbjct: 121 SLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEGKFAVELEFD 180

Query: 181 AKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLGLVE------------------- 240
           AKH PFYLSF+LK+   +   + +++   + +F   +G                      
Sbjct: 181 AKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSISGDGSINFS 240

Query: 241 -------------------------------VIQVRNIWHASFEGVSNFVSYGYQCK--- 300
                                          + +  NIWHASFEG SNFVSYGY+C+   
Sbjct: 241 IFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHASFEGASNFVSYGYRCRGSK 300

Query: 301 ----------------------------------------------VPAFDWDGEVRPNL 360
                                                         VPAF WDGEVRPNL
Sbjct: 301 FHENQDEAEVSRILVDPYAKILTASIPKTRGQGLGLPSKFLGQICNVPAFSWDGEVRPNL 360

Query: 361 PMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLDQEPIFQFDE 420
           PMEKLF+YRLNVK FT                 +T ++   + L V+  L  EPIF FDE
Sbjct: 361 PMEKLFIYRLNVKHFTVDKSSQLPIDTAGTFSGLTQKLLHFKKLGVNAVL-LEPIFPFDE 420

Query: 421 KEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTHTSVDGALQ 480
           K+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+THT++DGALQ
Sbjct: 421 KDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFTHTAMDGALQ 480

Query: 481 GIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCFVNASFLLQG 540
           GIDDS YYF NRVAN E ++           + LDSLR+WVTEFH+DGFCF+NAS LLQG
Sbjct: 481 GIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRHWVTEFHIDGFCFINASSLLQG 540

Query: 541 HHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCSDI 600
           HHGELLSRPPFVEAIAFDP+LSKTK++ADFWDPQE V KETRFPHWKRWAE+NSKF +DI
Sbjct: 541 HHGELLSRPPFVEAIAFDPLLSKTKIIADFWDPQESVPKETRFPHWKRWAELNSKFSNDI 600

Query: 601 RDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSNSNLASEL 659
           ++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS+S LASEL
Sbjct: 601 KEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSSSKLASEL 660

BLAST of IVF0006583 vs. NCBI nr
Match: KAA0057598.1 (isoamylase 2 [Cucumis melo var. makuwa] >TYK20996.1 isoamylase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1065 bits (2753), Expect = 0.0
Identity = 584/855 (68.30%), Postives = 607/855 (70.99%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKWTRL------------ 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT     K                
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  ---------ECYICYIGNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG----------- 120
                    + Y+    + G ++GQRLGIGGKSKEQRKVATYLFR  +G           
Sbjct: 61  VKSSHRNLSKAYVTSGISVG-KSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTG 120

Query: 121 --------------------------------------FGDFTPDGTTGATETPFVKTSE 180
                                                 F    PDGTTGATETPFVKTSE
Sbjct: 121 SAFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSE 180

Query: 181 GKFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------ 240
           GKFSVELEFDAKHTPFYLSFVLKF   +   + ++    + +F   +G            
Sbjct: 181 GKFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPSGLS 240

Query: 241 --------------------------------LVE------VIQVRNIWHASFEGVSNFV 300
                                           L+E      + +  NIWHASFEGVSNFV
Sbjct: 241 ISGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGVSNFV 300

Query: 301 SYGYQCK-------------------------------------------------VPAF 360
           SYGYQCK                                                 VPAF
Sbjct: 301 SYGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAF 360

Query: 361 DWDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWL 420
           DWDGEVRPNLPMEKLFVYRLNVKRFT                 +T ++   + L V+  L
Sbjct: 361 DWDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVL 420

Query: 421 DQEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVY 480
             EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVY
Sbjct: 421 -LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVY 480

Query: 481 THTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFC 540
           THTSVDGALQGIDDSSYYFANRVANLEER+           + LDSLRYWVTEFHVDGFC
Sbjct: 481 THTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFC 540

Query: 541 FVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWA 600
           FVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWA
Sbjct: 541 FVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWA 600

Query: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 658
           EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS
Sbjct: 601 EVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVS 660

BLAST of IVF0006583 vs. NCBI nr
Match: XP_008451396.1 (PREDICTED: isoamylase 2, chloroplastic [Cucumis melo])

HSP 1 Score: 1064 bits (2751), Expect = 0.0
Identity = 583/854 (68.27%), Postives = 606/854 (70.96%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTS------------------ISL 60
           MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKT                   +  
Sbjct: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTKYQSGKMDEARMLAHGESKVGA 60

Query: 61  EKWTRLECYICYI--GNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
            K +       Y+  G    ++GQRLGIGGKSKEQRKVATYLFR  +G            
Sbjct: 61  VKSSHRNLSKAYVTSGILVGKSGQRLGIGGKSKEQRKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PDGTTGATETPFVKTSEG
Sbjct: 121 AFTVNIEISSMQLVSTDETLLLSWRVYRSDSAPVTSNFQSSPPDGTTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------- 240
           KFSVELEFDAKHTPFYLSFVLKF   +   + ++    + +F   +G             
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKFPMGVDSGSSEIRSHRKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 -------------------------------LVE------VIQVRNIWHASFEGVSNFVS 300
                                          L+E      + +  NIWHASFEG SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASNFVS 300

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGYQCK                                                 VPAFD
Sbjct: 301 YGYQCKGLKSHENQDGLEVSRIVVDPYAKILAPSIPKSRDQGLGFPLKFLGQISIVPAFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLD 420
           WDGEVRPNLPMEKLFVYRLNVKRFT                 +T ++   + L V+  L 
Sbjct: 361 WDGEVRPNLPMEKLFVYRLNVKRFTIDKSSQLPADIAGTFSGLTKKLLHLKNLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT
Sbjct: 421 LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HTSVDGALQGIDDSSYYFANRVANLEER+           + LDSLRYWVTEFHVDGFCF
Sbjct: 481 HTSVDGALQGIDDSSYYFANRVANLEERSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE
Sbjct: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 658
           VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. NCBI nr
Match: XP_004136188.1 (isoamylase 2, chloroplastic [Cucumis sativus] >KGN44865.1 hypothetical protein Csa_016566 [Cucumis sativus])

HSP 1 Score: 1019 bits (2636), Expect = 0.0
Identity = 558/854 (65.34%), Postives = 590/854 (69.09%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKWTRLECYICYIGNFG- 60
           MATFLSSLVIQPFCV+NCGVTES KLA SDHLTYGQKT     K              G 
Sbjct: 1   MATFLSSLVIQPFCVYNCGVTESPKLAASDHLTYGQKTKYQFGKMDEARMLAHGENKVGA 60

Query: 61  -------------------WENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
                               ++GQRLGIGGKSKEQR+VATYLFR  +G            
Sbjct: 61  VKSSHRNLSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGKKGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PD TTGATETPFVKTSEG
Sbjct: 121 TFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTPNFESSPPDETTGATETPFVKTSEG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLG------------- 240
           KFSVELEFDAKHTPFYLSFVLK+   +   + ++    + +F   +G             
Sbjct: 181 KFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPLGLSI 240

Query: 241 -------------------------------LVE------VIQVRNIWHASFEGVSNFVS 300
                                          L+E      + +  NIWHASFEG S FVS
Sbjct: 241 SGDGSVNFSIFSSSAESLVLCLYNDSTSEKPLLELDLDPYINRSGNIWHASFEGASKFVS 300

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGYQCK                                                 VP FD
Sbjct: 301 YGYQCKGSKSHENQDGLEVSRIVVDPYAKILAPSIPKSSGQGLGLPSKFLGQISKVPTFD 360

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFTM-----------------TNQVSFRQILPVHFWLD 420
           WDGEV PNLPMEKLFVYRLNV+RFTM                 T ++   + L V+  L 
Sbjct: 361 WDGEVHPNLPMEKLFVYRLNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVL- 420

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVI+EVVYT
Sbjct: 421 LEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYT 480

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HTS +GALQGIDDSSYYF NRVANLEE++           + LDSLRYWVTEFHVDGFCF
Sbjct: 481 HTSGNGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCF 540

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           VNASFLL+GHHGELLSRPPFVEAIAFDP+LSKTKLVADFWDPQEL SKETRFPHWKRWAE
Sbjct: 541 VNASFLLRGHHGELLSRPPFVEAIAFDPLLSKTKLVADFWDPQELESKETRFPHWKRWAE 600

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 658
           VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 660

BLAST of IVF0006583 vs. NCBI nr
Match: XP_038898155.1 (isoamylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 520/853 (60.96%), Postives = 566/853 (66.35%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKWTR------------- 60
           MATFLSSLVIQPFCV+N GVTES KLAV DH TYGQK      K                
Sbjct: 1   MATFLSSLVIQPFCVYNSGVTESFKLAVGDHHTYGQKIKYHSGKMDEARMLAHGESRVGS 60

Query: 61  -------LECYICYIGNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
                  L       G    ++GQR G GGKSKE  KVATYLFR  +G            
Sbjct: 61  TKSSHRNLSKVYATSGILVGKSGQRFGTGGKSKEHVKVATYLFRTEFGDLVNVFVGKTGS 120

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    PD T GATETPFVKTS G
Sbjct: 121 GFMVNVEISSVQLASIEKTLLLSWGVYRSDSAIVTPNFQSSLPDETIGATETPFVKTSGG 180

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLGLVE---------- 240
           KF+VELEFDAKHTPFYLSF+LK+   +   + ++    + +F   +G             
Sbjct: 181 KFAVELEFDAKHTPFYLSFLLKYPVGVDSGSSEIRSHRKTSFTVPVGFGRGYPSPLGLSI 240

Query: 241 ----------------------------------------VIQVRNIWHASFEGVSNFVS 300
                                                   + +  +IWHASFE  SNFVS
Sbjct: 241 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDSYINRSGSIWHASFESASNFVS 300

Query: 301 YGYQCK------------------------------------------------VPAFDW 360
           YGY+CK                                                VPAFDW
Sbjct: 301 YGYRCKGSKFHENQDGVEVNRIVVDPYAKILTASVAKTLGQGLGLPKFLGQICKVPAFDW 360

Query: 361 DGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLDQ 420
           DGEV PNLPMEKLF YRLNVKRFT                 +T ++   + L V+  L  
Sbjct: 361 DGEVCPNLPMEKLFAYRLNVKRFTVDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVL-L 420

Query: 421 EPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTH 480
           EPIFQFDEKEG YFPFHFFSP NNYGPSG SISAINSMK MVK+LHANGVEVI+EVV+TH
Sbjct: 421 EPIFQFDEKEGLYFPFHFFSPANNYGPSGVSISAINSMKGMVKQLHANGVEVILEVVFTH 480

Query: 481 TSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCFV 540
           T++DGALQGIDDSSYYF NRVANLE ++           + LDSLRYWVTEFH+DGFCF+
Sbjct: 481 TAMDGALQGIDDSSYYFVNRVANLEGKSALNCNYPIVQQLLLDSLRYWVTEFHIDGFCFI 540

Query: 541 NASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEV 600
           NAS LL+GHHGE LSR PFVEAIAFDP+LSKTKLVADFWDPQELVS+ET FPHWKRWAEV
Sbjct: 541 NASSLLRGHHGEFLSRSPFVEAIAFDPLLSKTKLVADFWDPQELVSEETSFPHWKRWAEV 600

Query: 601 NSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFS 658
           NSKFC+D+RDFFRGEGLISSLATRLCGSGDVFS+GRGPAFSFNFI RNVGLPLVDLVSFS
Sbjct: 601 NSKFCNDVRDFFRGEGLISSLATRLCGSGDVFSNGRGPAFSFNFITRNVGLPLVDLVSFS 660

BLAST of IVF0006583 vs. NCBI nr
Match: KAG7014422.1 (Isoamylase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 914 bits (2363), Expect = 0.0
Identity = 499/854 (58.43%), Postives = 571/854 (66.86%), Query Frame = 0

Query: 1   MATFLSSLVIQPFCVHNCGVTESLKLAVSDHLTYGQKTSISLEKW--TRLECY------- 60
           MATFLSSL IQPFC +N G TES KLAV+D+ TYGQK +    K    R++ +       
Sbjct: 24  MATFLSSL-IQPFCGYNRGGTESFKLAVNDYHTYGQKMNYQSGKLDEARMQAHGESKVGF 83

Query: 61  -------ICYI----GNFGWENGQRLGIGGKSKEQRKVATYLFRRVWG------------ 120
                  +C      G    ++GQ+ G GGK++E RKVATYLFR  +G            
Sbjct: 84  TKSLHRNVCKAYAPSGISVGKSGQKFGTGGKTEEDRKVATYLFRTEFGDLVYAFVGKRGS 143

Query: 121 -------------------------------------FGDFTPDGTTGATETPFVKTSEG 180
                                                F    P+ T GATETPFV+TSEG
Sbjct: 144 GFLVSIEISSLKLASIDETLLLSWGVYRSNSAIVTPNFQSILPEETIGATETPFVRTSEG 203

Query: 181 KFSVELEFDAKHTPFYLSFVLKFQWVLIQAALKLEVIGRLAFLYLLGLVE---------- 240
           KF+VELEFDAKH PFYLSF+LK+   +   + +++   + +F   +G             
Sbjct: 204 KFAVELEFDAKHDPFYLSFLLKYPVGVDSGSSEIKSHRKTSFSVPVGFGRGYPSPLGLSI 263

Query: 241 ----------------------------------------VIQVRNIWHASFEGVSNFVS 300
                                                   + +  NIWH SFEG SNFVS
Sbjct: 264 SGDGSINFSIFSSTAESVVLCLYNDSTSEKPVLELDLDPYINRSGNIWHVSFEGASNFVS 323

Query: 301 YGYQCK-------------------------------------------------VPAFD 360
           YGY+C+                                                 VPAF 
Sbjct: 324 YGYRCRGSKFHENQDEAEVSRILVDPYAKILTASTPKTRGQGVGLPSKFLGQICNVPAFS 383

Query: 361 WDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQVSFRQILPVHFWLD 420
           WDGEVRPNLPMEKLF+YRLNVK FT                 +T ++   + L V+  L 
Sbjct: 384 WDGEVRPNLPMEKLFIYRLNVKHFTVDKSSQLPIDIAGTFSGLTQKLLHFKKLGVNAVL- 443

Query: 421 QEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYT 480
            EPIF FDEK+GPYFPFHFFSPTN Y PSGASIS++NS+KEMVKELHANGVEVI+EVV+T
Sbjct: 444 LEPIFPFDEKDGPYFPFHFFSPTNIYVPSGASISSLNSVKEMVKELHANGVEVILEVVFT 503

Query: 481 HTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSLRYWVTEFHVDGFCF 540
           HT++DGALQGIDDS YYF NRVAN E ++           + LDSLRYWVTEFH+DGFCF
Sbjct: 504 HTAMDGALQGIDDSCYYFVNRVANSEGKSALNCNHPIVQQLLLDSLRYWVTEFHIDGFCF 563

Query: 541 VNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAE 600
           +NAS LLQGHHGELLSRPPFVEAIAFDP+LSKTK++A+FWDPQELV KETRFPHWKRWAE
Sbjct: 564 INASSLLQGHHGELLSRPPFVEAIAFDPLLSKTKIIAEFWDPQELVPKETRFPHWKRWAE 623

Query: 601 VNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 658
           +NSKF +D+++FFRGEGL+SSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF
Sbjct: 624 LNSKFSNDMKEFFRGEGLVSSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSF 683

BLAST of IVF0006583 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 627.9 bits (1618), Expect = 9.5e-180
Identity = 332/691 (48.05%), Postives = 434/691 (62.81%), Query Frame = 0

Query: 89  FGDFTPDGTTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKF--------QWVLIQ 148
           F + + D  T  TET FVK+S  +  + LEFD K +PFYLSF LK         Q +L  
Sbjct: 157 FENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTH 216

Query: 149 A---------------------------ALKLEVIGRLAFLYLLGLVE------------ 208
                                       +       R +   +L L +            
Sbjct: 217 RDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELD 276

Query: 209 ----VIQVRNIWHASFEGVSNFVSYGYQC------------------------------- 268
               V +  ++WHAS +   +FV YGY+C                               
Sbjct: 277 LDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVS 336

Query: 269 --------KVPAFDWDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQ 328
                   K P+FDW  +V PN+P+EKL VYRLNVK FT                 +  +
Sbjct: 337 QKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEK 396

Query: 329 VSFRQILPVHFWLDQEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKEL 388
           VS  + L  +  L  EPIF F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+L
Sbjct: 397 VSHLKTLGTNAVL-LEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKL 456

Query: 389 HANGVEVIVEVVYTHTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSL 448
           H+ G+EV++EVV+THT+  GAL+GIDDSSYY+  R  +L+ ++           + L+SL
Sbjct: 457 HSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESL 516

Query: 449 RYWVTEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELV 508
           RYWVTEFHVDGFCF+NAS LL+G HGE LSRPP VEAIAFDP+L++TKL+AD WDP E++
Sbjct: 517 RYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMM 576

Query: 509 SKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFI 568
            KE RFPHWKRWAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I
Sbjct: 577 PKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYI 636

Query: 569 ARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVP 628
           +RN GL LVD+VSFS   LASELSWNCGEEG TN   VL++RLKQIRNF+F+ ++SLGVP
Sbjct: 637 SRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVP 696

Query: 629 VLNMGDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNF 659
           VLNMGDECG S+ GS     R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+F
Sbjct: 697 VLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDF 756

BLAST of IVF0006583 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 627.9 bits (1618), Expect = 9.5e-180
Identity = 332/691 (48.05%), Postives = 434/691 (62.81%), Query Frame = 0

Query: 89  FGDFTPDGTTGATETPFVKTSEGKFSVELEFDAKHTPFYLSFVLKF--------QWVLIQ 148
           F + + D  T  TET FVK+S  +  + LEFD K +PFYLSF LK         Q +L  
Sbjct: 157 FENSSQDSQTHTTETTFVKSSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGQEMLTH 216

Query: 149 A---------------------------ALKLEVIGRLAFLYLLGLVE------------ 208
                                       +       R +   +L L +            
Sbjct: 217 RDTDFCIPVGFTAGHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYDDSTTDKPALELD 276

Query: 209 ----VIQVRNIWHASFEGVSNFVSYGYQC------------------------------- 268
               V +  ++WHAS +   +FV YGY+C                               
Sbjct: 277 LDPYVNRTGDVWHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVS 336

Query: 269 --------KVPAFDWDGEVRPNLPMEKLFVYRLNVKRFT-----------------MTNQ 328
                   K P+FDW  +V PN+P+EKL VYRLNVK FT                 +  +
Sbjct: 337 QKYLGSLSKSPSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEK 396

Query: 329 VSFRQILPVHFWLDQEPIFQFDEKEGPYFPFHFFSPTNNYGPSGASISAINSMKEMVKEL 388
           VS  + L  +  L  EPIF F E++GPYFPFHFFSP + YGPS +  SA+NSMK MVK+L
Sbjct: 397 VSHLKTLGTNAVL-LEPIFSFSEQKGPYFPFHFFSPMDIYGPSNSLESAVNSMKVMVKKL 456

Query: 389 HANGVEVIVEVVYTHTSVDGALQGIDDSSYYFANRVANLEERT-----------ITLDSL 448
           H+ G+EV++EVV+THT+  GAL+GIDDSSYY+  R  +L+ ++           + L+SL
Sbjct: 457 HSEGIEVLLEVVFTHTADSGALRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESL 516

Query: 449 RYWVTEFHVDGFCFVNASFLLQGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELV 508
           RYWVTEFHVDGFCF+NAS LL+G HGE LSRPP VEAIAFDP+L++TKL+AD WDP E++
Sbjct: 517 RYWVTEFHVDGFCFINASSLLRGVHGEQLSRPPLVEAIAFDPLLAETKLIADCWDPLEMM 576

Query: 509 SKETRFPHWKRWAEVNSKFCSDIRDFFRGEGLISSLATRLCGSGDVFSDGRGPAFSFNFI 568
            KE RFPHWKRWAE+N+++C ++R+F RG G++S LATR+CGSGDVF+DGRGPAFSFN+I
Sbjct: 577 PKEVRFPHWKRWAELNTRYCRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNYI 636

Query: 569 ARNVGLPLVDLVSFSNSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVP 628
           +RN GL LVD+VSFS   LASELSWNCGEEG TN   VL++RLKQIRNF+F+ ++SLGVP
Sbjct: 637 SRNSGLSLVDIVSFSGPELASELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVP 696

Query: 629 VLNMGDECGQSSGGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNF 659
           VLNMGDECG S+ GS     R+ F+W+LL + FGTQ TQFI+F++S R+RR D+FQ R+F
Sbjct: 697 VLNMGDECGISTRGSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDF 756

BLAST of IVF0006583 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 253.1 bits (645), Expect = 6.4e-67
Identity = 183/562 (32.56%), Postives = 265/562 (47.15%), Query Frame = 0

Query: 170 HASFEGVSNFVS--YG-YQCKVPAFDWDGEVR-PNLPMEKLFVYRLNVKRFT-------- 229
           H+SF   S   +  YG Y  +   FDW  + + PN+P + L +Y +NV+ FT        
Sbjct: 196 HSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMD 255

Query: 230 ---------MTNQVSFRQILPVHFWLDQEPIFQFDEKE--------------GPYFPFHF 289
                       ++   Q L ++  ++  P+F+FDE E                Y   +F
Sbjct: 256 PAIGGSYLGFIEKIPHLQDLGINA-VELLPVFEFDELELQRRSNPRDHMVNTWGYSTVNF 315

Query: 290 FSPTNNYGP-SGASISAINSMKEMVKELHANGVEVIVEVVYTHTS-------VDGALQGI 349
           F+P + Y    G  I A    KEMVK LH+ G+EVI++VVY HT+          + +GI
Sbjct: 316 FAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGI 375

Query: 350 DDSSYYFA---NRVANLE------------ERTITLDSLRYWVTEFHVDGFCFVNASFLL 409
           D+  YY     N++ N                 + LDSLR+WVTE+HVDGF F  AS L 
Sbjct: 376 DNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLC 435

Query: 410 QGHHGELLSRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSKFCS 469
           +   G  LS PP + AIA D +LS+ K++A+ WD   L     +FP+W RWAE N  +  
Sbjct: 436 RATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLY-LVGKFPNWDRWAEWNGMYRD 495

Query: 470 DIRDFFRGE-GLISSLATRLCGSGDVFS-DGRGPAFSFNFIARNVGLPLVDLVSFS---- 529
           D+R F +G+ G+  S ATR+ GS D++  + R P    NF+  + G  L DLVS++    
Sbjct: 496 DVRRFIKGDSGMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHN 555

Query: 530 -------NSNLASELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGDEC 589
                  N       SWNCG EG T    +   R +Q++NF   L +S G P++ MGDE 
Sbjct: 556 EANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEY 615

Query: 590 GQSS-GGSVAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRR-FDLFQSRNFLKGENI 649
           G +  G + ++    S N    K     +   F  F    + R    + +  NFL    I
Sbjct: 616 GHTRYGNNNSYGHDTSLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEI 675

Query: 650 DWFDNNQSPPQWEDASCKFLAVMLRADKEENESITENPKTRSDIFMAFNASDQSESVALP 659
            W ++N     W+++  KFLA  L      ++ I        DI++AFNA D      +P
Sbjct: 676 TWHEDN-----WDNSESKFLAFTL------HDGIGGR-----DIYVAFNAHDYFVKALIP 735

BLAST of IVF0006583 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 226.1 bits (575), Expect = 8.4e-59
Identity = 174/563 (30.91%), Postives = 254/563 (45.12%), Query Frame = 0

Query: 186 CKVPA----FDWDGEVRPNLPMEKLFVYRLNVKRFTM--TNQVSFR-------------- 245
           C VP     FDW+G++   LP + L +Y ++V+ FT   ++++ F               
Sbjct: 194 CMVPTREEEFDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLK 253

Query: 246 -------QILPVHFWLDQEPIFQFDEKEGP-------YFPFHFFSPTNNYGPSGASI--- 305
                  +++P H + + E  + ++   G        Y    FFSP   Y  + ++    
Sbjct: 254 ELGINCIELMPCHEFNELE-YYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAG 313

Query: 306 SAINSMKEMVKELHANGVEVIVEVVYTHTSVDG------ALQGIDDSSYYF--------- 365
            AIN  K +VKE H  G+EVI++VV  HT+         + +G+D+S YY          
Sbjct: 314 RAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYN 373

Query: 366 ---ANRVANLEE---RTITLDSLRYWVTEFHVDGFCFVNASFLLQGH------------- 425
                   N      R   LD LRYWVTE HVDGF F   S + +               
Sbjct: 374 YSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADV 433

Query: 426 HGELL------SRPPFVEAIAFDPILSKTKLVADFWDPQELVSKETRFPHWKRWAEVNSK 485
            G+LL      S PP ++ I+ DPIL   KL+A+ WD   L  +   FPHW  W+E N K
Sbjct: 434 EGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLY-QVGMFPHWGIWSEWNGK 493

Query: 486 FCSDIRDFFRG-EGLISSLATRLCGSGDVFSDGRGPAFSFNFIARNVGLPLVDLVSFSN- 545
           F   +R F +G +G   + A  LCGS +++  GR P  S NFI  + G  L DLV+++N 
Sbjct: 494 FRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGRKPWHSINFICAHDGFTLADLVTYNNK 553

Query: 546 SNLAS----------ELSWNCGEEGPTNTLKVLEKRLKQIRNFIFVLFVSLGVPVLNMGD 605
           +NLA+            SWNCGEEG   ++ V   R +Q+RNF   L VS GVP++ MGD
Sbjct: 554 NNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVSLMVSQGVPMIYMGD 613

Query: 606 ECGQSSGGS----VAFNDRRSFNWDLLKTDFGTQTTQFIAFLSSFRSRRFDLFQSRNFLK 656
           E G + GG+       N    F WD  K +  +   +F   L  FR    +     +F  
Sbjct: 614 EYGHTKGGNNNTYCHDNYMNYFRWD-KKEEAHSDFFRFCRILIKFRD-ECESLGLNDFPT 673

BLAST of IVF0006583 vs. TAIR 10
Match: AT3G20440.1 (Alpha amylase family protein )

HSP 1 Score: 45.8 bits (107), Expect = 1.5e-04
Identity = 26/121 (21.49%), Postives = 57/121 (47.11%), Query Frame = 0

Query: 253 HFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVIVEVVYTHTSVD-----GALQGID 312
           +FF+ ++ YG         +  K +V E H  G+ V +++V+++ + D         G +
Sbjct: 426 NFFAASSRYGTP-------DDFKRLVDEAHGLGLLVFLDIVHSYAAADQMVGLSLFDGSN 485

Query: 313 DSSYYFANR------------VANLEERTITLDSLRYWVTEFHVDGFCFVNASFLLQGHH 357
           D  +++  R              +L+     + +L +W+TE+ VDG+ F + + ++  H+
Sbjct: 486 DCYFHYGKRGHHKHWGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHN 539