IVF0006218 (gene) Melon (IVF77) v1

Overview


Name	IVF0006218
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	sterol 14-demethylase
Location	chr07: 23132067 .. 23133828 (+)
RNA-Seq Expression	IVF0006218
Synteny	IVF0006218

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCTGATAACAACAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCCGCTCTTCTCAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTCCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCCAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCCGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGTCAGTATCATAGATCTAATAAATTGATAGGAATTTAAGAATGAAAATGAAAGGACTATAATATTCATATTCATTTAATGCAAGCTTCAGATAAGGATTGTAAATGTTAGGATTCTCTAAGAACCTGTGCTCTGTCTAGTTTTACTGATCTCCTTGCTAATGACAATGCATGCTAAATTCAACATCCATCCTACTTTTCTGATGATTACGTCCAAATCTTTGATTTCCAGTCTCTTTGTTTCTACATTATTCTTTAGAATCTGTGTAACCTTGGGATGTGGTGGGGTATTTTGAGCAGGATTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTCGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCCTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAGATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATCGACTCAAAGTATAAAGATGGGCGGTCAACAACAGATTCCGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACTAGTTCTATTACCTCAACTTGGACTGGCGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTCTTAGAGGAACAGCAAAGACTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCACTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAAGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAACCGACTTCCTCATGTGTATAAGGATCCCGACAGGTATGATCCAGACAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCATTCTCCTACATCTCGTTCGGAGGTGGTAGGCATGGGTGCCTCGGCGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCAGTTAGTTAA

mRNA sequence

ATGGAACCTGATAACAACAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCCGCTCTTCTCAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTCCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCCAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCCGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGATTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTCGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCCTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAGATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATCGACTCAAAGTATAAAGATGGGCGGTCAACAACAGATTCCGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACTAGTTCTATTACCTCAACTTGGACTGGCGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTCTTAGAGGAACAGCAAAGACTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCACTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAAGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAACCGACTTCCTCATGTGTATAAGGATCCCGACAGGTATGATCCAGACAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCATTCTCCTACATCTCGTTCGGAGGTGGTAGGCATGGGTGCCTCGGCGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCAGTTAGTTAA

Coding sequence (CDS)

ATGGAACCTGATAACAACAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCCGCTCTTCTCAATTCCAGATCTAACAAACGGGCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTCCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCCAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCCGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGATTTCTTCTCTAAGTGGGGTGACAGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTCGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCCTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAGATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATCGACTCAAAGTATAAAGATGGGCGGTCAACAACAGATTCCGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACTAGTTCTATTACCTCAACTTGGACTGGCGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTCTTAGAGGAACAGCAAAGACTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCTATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCACTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAAGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTCGCTAACCGACTTCCTCATGTGTATAAGGATCCCGACAGGTATGATCCAGACAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCATTCTCCTACATCTCGTTCGGAGGTGGTAGGCATGGGTGCCTCGGCGAACCCTTCGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCTTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGGAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCAGTTAGTTAA

Protein sequence

MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSVS

Homology

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BLAST of IVF0006218 vs. ExPASy Swiss-Prot
Match: Q9SAA9 (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 3.5e-237
Identity = 401/488 (82.17%), Postives = 445/488 (91.19%), Query Frame = 0


Query: 1   MEPDN-NKFFNVGLLLLATLLLAKLISALLNSRS-NKRAPPTVKG-IPFIGGLLRFLKGP 60
           ME D+ NK    GL+++ATL++AKLI +   S S  KR PPT+K   P +G L++FLKGP
Sbjct: 1   MELDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGP 60

Query: 61  IVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG 120
           I+MLR+EYPKLGSVFTVNL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG
Sbjct: 61  IIMLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG 120

Query: 121 VVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLI 180
           VVFDVDYS+RQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LI
Sbjct: 121 VVFDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLI 180

Query: 181 ILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADI 240
           ILTASRCLLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++I
Sbjct: 181 ILTASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEI 240

Query: 241 FAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWT 300
           FAKII SRK +G ++NDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWT
Sbjct: 241 FAKIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWT 300

Query: 301 GAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRS 360
           GAYL+ +KEY SA L+EQ+ L+ KHGDKIDHDILSEMD LYRCIKEALRLHPPLIMLMR+
Sbjct: 301 GAYLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRA 360

Query: 361 SHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGP 420
           SHSDFSVT R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG 
Sbjct: 361 SHSDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGA 420

Query: 421 FSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMV 480
           FSYI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMV
Sbjct: 421 FSYIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMV 480

Query: 481 RYKRRKLS 486
           RYKRR+LS
Sbjct: 481 RYKRRQLS 488

[Close]

BLAST of IVF0006218 vs. ExPASy Swiss-Prot
Match: P93846 (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 766.1 bits (1977), Expect = 2.3e-220
Identity = 360/473 (76.11%), Postives = 422/473 (89.22%), Query Frame = 0


Query: 14  LLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFT 73
           L++AT++  KL+ +  +    KR PPT+ G P +GGL++F++GPI M+R++Y  LGSVFT
Sbjct: 18  LVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIREQYAALGSVFT 77

Query: 74  VNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRF 133
           V ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDVDYS+RQEQFRF
Sbjct: 78  VPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDVDYSVRQEQFRF 137

Query: 134 FTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDK 193
           FTEALR  KL+ YVDQMV EAE++FSKWG+SG VDLKYELEHLIILTASRCLLG EVR+K
Sbjct: 138 FTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREK 197

Query: 194 LFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDN 253
           LF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II SRK +G S+ 
Sbjct: 198 LFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATIIKSRKASGQSEE 257

Query: 254 DMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLE 313
           DMLQCFIDSKYK+GR TT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L  K+Y +  +E
Sbjct: 258 DMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYMLRFKQYFAEAVE 317

Query: 314 EQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYD 373
           EQ+ +M +HGDKIDHDIL+EMD LYRCIKEALRLHPPLIML+R SHSDF+VTT+EGKEYD
Sbjct: 318 EQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDFTVTTKEGKEYD 377

Query: 374 IPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGE 433
           IPKGHI+ATSP+FANRLPH+YK+PD YDPDRF PGR+EDKAAG FSYISFGGGRHGCLGE
Sbjct: 378 IPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLGE 437

Query: 434 PFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 487
           PFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG+VMV YKRRKL V
Sbjct: 438 PFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRRKLVV 490

[Close]

BLAST of IVF0006218 vs. ExPASy Swiss-Prot
Match: P93596 (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 5.1e-220
Identity = 362/451 (80.27%), Postives = 409/451 (90.69%), Query Frame = 0


Query: 36  RAPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFK 95
           R PPT+ G P +GGLLRFL+GPI ++R EY +LG VFTV +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 96  ASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAE 155
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY +RQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 156 DFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVM 215
           ++FSKWG+SG VDLKYELEHLIILTASRCLLG EVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 216 FPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEV 275
           FPYLPIPAHRRRDQAR++LA+IFA II SRK +G S+ DMLQCFIDSKYK+GR TT+SEV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 276 TGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMD 335
           TGLLIAALFAGQHTSSITSTWTGAYLL  ++Y +  +EEQ+ +M +HGDKIDHDIL+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 336 NLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYK 395
            LYRCIKEALRLHPPLIML+R SHSDFSVTTREGKE+DIPKGHI+ATSPAFANRLPH++K
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 396 DPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELEL 455
           +PD YDPDRFA GR+EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 456 ISPFPEIDWNAMVVGVKGKVMVRYKRRKLSV 487
           +SPFPE DWNAMVVG+KG+VMV YKRRKL V
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKLIV 451

[Close]

BLAST of IVF0006218 vs. ExPASy Swiss-Prot
Match: Q1ZXH9 (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 9.4e-105
Identity = 213/474 (44.94%), Postives = 295/474 (62.24%), Query Frame = 0


Query: 16  LATLLLAKL-ISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTV 75
           +A L++A L I A   S SN   PP V+ IPFIG   +F K P+ ++R  Y +LG +FT+
Sbjct: 5   IAVLVIAILVIFAFKKSPSN--IPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIFTL 64

Query: 76  NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFF 135
           +L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  I  EQ RF 
Sbjct: 65  HLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLRFV 124

Query: 136 TEALRVTKLKGYVDQMVREAEDFF-SKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDK 195
              L +++LK  V  +  E E +F +KWGDSGE+DL YE+  L ILTASRCL+G  +   
Sbjct: 125 KNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSINKS 184

Query: 196 L--FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS-RKCNGS 255
           L     ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+    
Sbjct: 185 LGQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRRSTDD 244

Query: 256 SDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSA 315
           S +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + EY   
Sbjct: 245 SVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFDE 304

Query: 316 VLEEQQRLMGKHGD-KIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREG 375
             ++   ++ K    +I+ D L  M+ L   I+E LRLHPPLI LMR       +T  E 
Sbjct: 305 TQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMTPMEY 364

Query: 376 KEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHG 435
           K   IP GHI+A SP    RLP VYK+PD ++P RF     EDK   PFS+I+FGGG+HG
Sbjct: 365 KGKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDV---EDKT--PFSFIAFGGGKHG 424

Query: 436 CLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRK 484
           C GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 CPGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

[Close]

BLAST of IVF0006218 vs. ExPASy Swiss-Prot
Match: Q4PJW3 (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 5.5e-89
Identity = 184/477 (38.57%), Postives = 269/477 (56.39%), Query Frame = 0


Query: 9   FNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKL 68
           F + L+ L  L +  L   L      K  P  V  IPF+G  + F K PI  L   Y K 
Sbjct: 34  FTLSLVYLFRLAVGHLAPPL--PTGAKSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKY 93

Query: 69  GSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVY-QFNVPTFGPGVVFDVDYSIR 128
           G VF+  ++ K  T+L+G E +A  F +   DL+ +EVY +   P FG GV +DV  ++ 
Sbjct: 94  GPVFSFTMVGKTFTYLLGSEAAALLFNSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVF 153

Query: 129 QEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLG 188
            EQ +     L +   + +V  + +E +++F  WG+SGE +L   L  LIILTAS CL G
Sbjct: 154 LEQKKMLKSGLNIAHFRQHVSIIEKETKEYFKSWGESGEKNLFEALSELIILTASHCLHG 213

Query: 189 SEVRDKLFADVSALFHDLDNGMLPISVMFP-YLPIPAHRRRDQARSKLADIFAKIIASRK 248
            E+R +L   V+ L+ DLD G    + + P +LP+P+ RRRD+A  ++ +IF K I  R+
Sbjct: 214 KEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRR 273

Query: 249 CNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKE 308
            +G   +D+LQ  ++S YKDGR  TD EV G+LI  L AGQHTSS TS W G +L   K 
Sbjct: 274 ESGEKIDDILQTLLESTYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKT 333

Query: 309 YMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTT 368
                  EQ+ + G++   + +D L +++ L RCIKE LRL PP++ +MR + +  +V  
Sbjct: 334 LQEKCFLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA- 393

Query: 369 REGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGG 428
                Y IP GH +  SP    RL   + +   ++PDR+    D   +   F+Y+ FG G
Sbjct: 394 ----GYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYL--EDSPASGEKFAYVPFGAG 453

Query: 429 RHGCLGEPFAYLQIKAIWSQLLRNFELELISP-FPEIDWNAMVVGVKGKVMVRYKRR 483
           RH C+GE FAY+QIK IWS +LR +E +LI   FP +++  M +    K ++RYKRR
Sbjct: 454 RHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

[Close]

BLAST of IVF0006218 vs. ExPASy TrEMBL
Match: A0A5A7TLX5 (Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002430 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-283
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 488
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of IVF0006218 vs. ExPASy TrEMBL
Match: A0A1S3BXE7 (sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 1.5e-283
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 488
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of IVF0006218 vs. ExPASy TrEMBL
Match: A0A0A0KYP3 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 971.1 bits (2509), Expect = 1.7e-279
Identity = 481/487 (98.77%), Postives = 483/487 (99.18%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KFFNVGLL+LATLLLAKLISALLNSRSNKR PPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQ LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 488
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of IVF0006218 vs. ExPASy TrEMBL
Match: A0A6J1J0F8 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.1e-271
Identity = 466/487 (95.69%), Postives = 479/487 (98.36%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPD NKF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPD-NKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 488
           RRKLSV+
Sbjct: 481 RRKLSVN 486

[Close]

BLAST of IVF0006218 vs. ExPASy TrEMBL
Match: A0A6J1GUN6 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV=1)

HSP 1 Score: 939.5 bits (2427), Expect = 5.6e-270
Identity = 464/487 (95.28%), Postives = 478/487 (98.15%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPD NKF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPD-NKFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 488
           RRKLSV+
Sbjct: 481 RRKLSVN 486

[Close]

BLAST of IVF0006218 vs. NCBI nr
Match: XP_008454145.1 (PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethylase [Cucumis melo var. makuwa] >TYK29631.1 sterol 14-demethylase [Cucumis melo var. makuwa])

HSP 1 Score: 983 bits (2540), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of IVF0006218 vs. NCBI nr
Match: NP_001295809.1 (sterol 14-demethylase [Cucumis sativus] >AIY67848.1 cytochrome P450 [Cucumis sativus] >KGN52966.1 hypothetical protein Csa_014475 [Cucumis sativus])

HSP 1 Score: 969 bits (2505), Expect = 0.0
Identity = 481/487 (98.77%), Postives = 483/487 (99.18%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KFFNVGLL+LATLLLAKLISALLNSRSNKR PPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQ LMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSVS
Sbjct: 481 RRKLSVS 487

[Close]

BLAST of IVF0006218 vs. NCBI nr
Match: XP_038882542.1 (sterol 14-demethylase [Benincasa hispida])

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 470/487 (96.51%), Postives = 479/487 (98.36%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           M+PDN KFFNVGLL+LATL LAKLIS +LNSRSNKR PPTVKG PFIGGLLRFLKGPI+M
Sbjct: 1   MDPDN-KFFNVGLLILATLFLAKLISVVLNSRSNKRPPPTVKGFPFIGGLLRFLKGPIMM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSS+NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSENDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYM+AVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLRHKEYMAAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DF+VTTREGKEYDIPKGHIIATSPAFANRL HVYKDP+RYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFTVTTREGKEYDIPKGHIIATSPAFANRLSHVYKDPNRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWS LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSVS
Sbjct: 481 RRKLSVS 486

[Close]

BLAST of IVF0006218 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 466/487 (95.69%), Postives = 479/487 (98.36%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSV+
Sbjct: 481 RRKLSVN 486

[Close]

BLAST of IVF0006218 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 941 bits (2432), Expect = 0.0
Identity = 466/487 (95.69%), Postives = 479/487 (98.36%), Query Frame = 0


Query: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKRAPPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGDSGEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLSVS 487
           RRKLSV+
Sbjct: 481 RRKLSVN 486

[Close]

BLAST of IVF0006218 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 822.0 bits (2122), Expect = 2.5e-238
Identity = 401/488 (82.17%), Postives = 445/488 (91.19%), Query Frame = 0


Query: 1   MEPDN-NKFFNVGLLLLATLLLAKLISALLNSRS-NKRAPPTVKG-IPFIGGLLRFLKGP 60
           ME D+ NK    GL+++ATL++AKLI +   S S  KR PPT+K   P +G L++FLKGP
Sbjct: 1   MELDSENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGP 60

Query: 61  IVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG 120
           I+MLR+EYPKLGSVFTVNL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG
Sbjct: 61  IIMLREEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPG 120

Query: 121 VVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLI 180
           VVFDVDYS+RQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+SGEVD+K ELE LI
Sbjct: 121 VVFDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLI 180

Query: 181 ILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADI 240
           ILTASRCLLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++I
Sbjct: 181 ILTASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEI 240

Query: 241 FAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWT 300
           FAKII SRK +G ++NDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWT
Sbjct: 241 FAKIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWT 300

Query: 301 GAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRS 360
           GAYL+ +KEY SA L+EQ+ L+ KHGDKIDHDILSEMD LYRCIKEALRLHPPLIMLMR+
Sbjct: 301 GAYLMRYKEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRA 360

Query: 361 SHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGP 420
           SHSDFSVT R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG 
Sbjct: 361 SHSDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGA 420

Query: 421 FSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMV 480
           FSYI+FGGGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMV
Sbjct: 421 FSYIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMV 480

Query: 481 RYKRRKLS 486
           RYKRR+LS
Sbjct: 481 RYKRRQLS 488

[Close]

BLAST of IVF0006218 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 129.4 bits (324), Expect = 7.8e-30
Identity = 116/465 (24.95%), Postives = 199/465 (42.80%), Query Frame = 0


Query: 19  LLLAKLISALLNSRSNK-RAPPTVKGIPFIGGLLR-FLKGPIVMLRKEYPKLGSVFTVNL 78
           L L + I+ +  S S K   PP   G P++G   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 79  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 138
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 139 ALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 198
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 199 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSD 258
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 259 NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVL 318
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 319 EEQQ--RLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGK 378
           EEQ   R   K G+ +  +   +M   YR I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 379 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 438
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 436

Query: 439 GCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG 474
            C G   A L+I  +   L   +   ++ P   I +    +   G
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNG 454

[Close]

BLAST of IVF0006218 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-28
Identity = 114/445 (25.62%), Postives = 193/445 (43.37%), Query Frame = 0


Query: 19  LLLAKLISALLNSRSNK-RAPPTVKGIPFIGGLLR-FLKGPIVMLRKEYPKLGSVFTVNL 78
           L L + I+ +  S S K   PP   G P++G   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 79  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 138
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 139 ALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 198
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 199 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSD 258
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 259 NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVL 318
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 319 EEQQ--RLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGK 378
           EEQ   R   K G+ +  +   +M   YR I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 379 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 438
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 434

Query: 439 GCLGEPFAYLQIKAIWSQLLRNFEL 454
            C G   A L+I  +   L   + L
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRL 434

[Close]

BLAST of IVF0006218 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 117.1 bits (292), Expect = 4.0e-26
Identity = 112/484 (23.14%), Postives = 202/484 (41.74%), Query Frame = 0


Query: 13  LLLLATLLLAKLISALLNSR----SNKRAPPTVKGIPFIGGLLR-FLKGPIVMLRKEYPK 72
           L L A  L    +  L++ R    S    PP   G P++G   + + + P V  + +  +
Sbjct: 8   LTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKR 67

Query: 73  LGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIR 132
            GSVF  ++L      +  PE +A F   ++S L +           G   +F       
Sbjct: 68  YGSVFKTHVLGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYH 127

Query: 133 QEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLG 192
            +  +    A     ++  V  +   A+D    W +   ++   E++      A   + G
Sbjct: 128 AKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW-EGTMINTYQEMKTYTFNVALLSIFG 187

Query: 193 SEVRDKLF-ADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRK 252
            +  + L+  D+   ++ L+ G   + V    LP     +  +AR +L+ I A+I++ R+
Sbjct: 188 KD--EVLYREDLKRCYYILEKGYNSMPV---NLPGTLFHKSMKARKELSQILARILSERR 247

Query: 253 CNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKE 312
            NGSS ND+L  F+  K       TD ++   +I  +FA + T++   +W   YL  +  
Sbjct: 248 QNGSSHNDLLGSFMGDK----EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPN 307

Query: 313 YMSAVLEEQQ--RLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSV 372
            + AV EEQ   R   + G+ +      +M    R I+E LR+   L    R +  D   
Sbjct: 308 VLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV-- 367

Query: 373 TTREGKEYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYIS 432
              E + Y IPKG  +   P F N      ++ +P ++DP RF      + A  P +++ 
Sbjct: 368 ---EYEGYLIPKGWKVL--PLFRNIHHSADIFSNPGKFDPSRF------EVAPKPNTFMP 427

Query: 433 FGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRR 487
           FG G H C G   A L++  +   L   +   ++     I +    +   G  +V  ++ 
Sbjct: 428 FGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARKP 467

[Close]

BLAST of IVF0006218 vs. TAIR 10
Match: AT2G29090.1 (cytochrome P450, family 707, subfamily A, polypeptide 2 )

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-25
Identity = 102/451 (22.62%), Postives = 174/451 (38.58%), Query Frame = 0


Query: 36  RAPPTVKGIPFIGGLLR-FLKGPIVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVS---- 95
           R PP   G+P+IG  LR + + P         K G +F  ++L      +  PE +    
Sbjct: 48  RLPPGSMGLPYIGETLRLYTENPNSFFATRQNKYGDIFKTHILGCPCVMISSPEAARMVL 107

Query: 96  ---AHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYV 155
              AH FK +     ++ +        GP  +F           R    +   + L+  V
Sbjct: 108 VSKAHLFKPTYPPSKERMI--------GPEALFFHQGPYHSTLKRLVQSSFMPSALRPTV 167

Query: 156 DQMVREAEDFFSKWGDSGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDN 215
             +        S W     ++    ++      A     G +        +  L+  L+ 
Sbjct: 168 SHIELLVLQTLSSWTSQKSINTLEYMKRYAFDVAIMSAFGDKEEPTTIDVIKLLYQRLER 227

Query: 216 G--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDNDMLQCFIDSKYK 275
           G   +P+      LP     +  +AR +L++   K+I  R+ NG  +  +L   + +K +
Sbjct: 228 GYNSMPLD-----LPGTLFHKSMKARIELSEELRKVIEKRRENGREEGGLLGVLLGAKDQ 287

Query: 276 DGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLEEQ---QRLMGKH 335
                +DS++   +I  +FA   T++   TW   YL  H   +  V  EQ   ++ + K 
Sbjct: 288 KRNGLSDSQIADNIIGVIFAATDTTASVLTWLLKYLHDHPNLLQEVSREQFSIRQKIKKE 347

Query: 336 GDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYDIPKGHIIAT 395
             +I  +   +M    R I+E LR    L    R +  D      E   Y IPKG  +  
Sbjct: 348 NRRISWEDTRKMPLTTRVIQETLRAASVLSFTFREAVQDV-----EYDGYLIPKGWKVLP 407

Query: 396 SPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKA 455
                +     + DP+++DP RF      + A  P++Y+ FG G H C G   A L++  
Sbjct: 408 LFRRIHHSSEFFPDPEKFDPSRF------EVAPKPYTYMPFGNGVHSCPGSELAKLEMLI 467

Query: 456 IWSQLLRNFELELISPFPEIDWNAMVVGVKG 474
           +   L  +F  E+I     I +    V  KG
Sbjct: 468 LLHHLTTSFRWEVIGDEEGIQYGPFPVPKKG 474

The following BLAST results are available for this feature:

BLAST of IVF0006218 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAA9	3.5e-237	82.17	Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1	[more]
P93846	2.3e-220	76.11	Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1	[more]
P93596	5.1e-220	80.27	Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP...	[more]
Q1ZXH9	9.4e-105	44.94	Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN...	[more]
Q4PJW3	5.5e-89	38.57	Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1	[more]

BLAST of IVF0006218 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TLX5	1.5e-283	100.00	Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65...	[more]
A0A1S3BXE7	1.5e-283	100.00	sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1	[more]
A0A0A0KYP3	1.7e-279	98.77	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1	[more]
A0A6J1J0F8	5.1e-271	95.69	sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1	[more]
A0A6J1GUN6	5.6e-270	95.28	sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV...	[more]

BLAST of IVF0006218 vs. NCBI nr


Match Name	Identity	Description
XP_008454145.1	100.00	PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethyl...	[more]
NP_001295809.1	98.77	sterol 14-demethylase [Cucumis sativus] >AIY67848.1 cytochrome P450 [Cucumis sat...	[more]
XP_038882542.1	96.51	sterol 14-demethylase [Benincasa hispida]	[more]
XP_023527569.1	95.69	sterol 14-demethylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980879.1	95.69	sterol 14-demethylase-like [Cucurbita maxima]	[more]

BLAST of IVF0006218 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11680.1	2.5e-238	82.17	CYTOCHROME P450 51G1	[more]
AT5G45340.1	7.8e-30	24.95	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G45340.2	1.5e-28	25.62	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	4.0e-26	23.14	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT2G29090.1	1.5e-25	22.62	cytochrome P450, family 707, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 334..350 score: 46.61 coord: 59..82 score: 35.9 coord: 388..406 score: 28.34 coord: 276..302 score: 37.32 coord: 430..448 score: 43.52 coord: 414..430 score: 34.39 coord: 372..386 score: 37.18 coord: 37..54 score: 29.91
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 421..430 score: 40.95 coord: 339..350 score: 40.69 coord: 285..302 score: 32.57 coord: 430..441 score: 38.9
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 38..457 e-value: 8.7E-66 score: 222.6
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 33..486 e-value: 5.1E-122 score: 409.9
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 33..482
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 11..482 e-value: 7.2E-35 score: 118.6
None	No IPR available	PANTHER	PTHR24286:SF251	STEROL 14-DEMETHYLASE	coord: 10..481
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 10..481
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 423..432

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0006218.1	IVF0006218.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen