IVF0005421 (gene) Melon (IVF77) v1

Overview
NameIVF0005421
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr09: 13251278 .. 13252594 (+)
RNA-Seq ExpressionIVF0005421
SyntenyIVF0005421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTTTTGTGCTGCTAATGAGCCCTCTATCAGATTTATCCGAGAATGTCTTCAGAAGTTTGGTGAGCTTTCAGGTTTGTTCGCAAATCCTAGGAAAAGCTCTATTTTTGTTGCAGGAGTTAATAATGAGAATGCTTCTCATCTGGCTACTTGTATGGGTTTTGTCCGTGGTAATCTCTCTGTTCGTTATCTTGGCCTTCCTCTTTTGGCGGGTCGATTACGTTCTAATGATTATGCTCCTCTGATTCAGCGTATCACTAGCAGGATTCGTTCTTGGACCGCTCGAGTTCTTTCGTTTGCAGGTAGACTGCAGCTTGTTCATTCTGTGCTTCGTAGTTTTCAAGTGTACTGGGCTAGTGTGTTTGTTCTTCCTGCGTATGTGCACAATGAGGTGGACAAGATTCTCAGATCTTATCTTTGGAGGGGTAAGGAGGAAGGTAGAGGAGGTATTAAGGTGGCTTGGGTGGATGTGTGTCTTCCTTTGAGGAGGGCGGTCTTGGTATTCGAGATGGCCCTTCTTGGAATATTGCGAGTACTTTGAAGATCTTGTGGCTTATGTTAACAAATTCGGGGTCTCTTTGGGTGGCTTGGGTGGAAGCTTATATACTGAAAGGGAGGTCATTGTGGGATGTGGATAGTAGAGTGGGTAAATCTTGGTGTCTTCGGGCGATCTTACGTAAGCGAGAGAAGCTGAAGCATCTTGTAAGGATGAAGGTGGGAAATGGTAATAGTTTTAGAGTTTGGCTTGATCCGTGGTTGCCGGAGGGTGCCATTTTGGAGCAGGTTGGGGAGAGAGTGATGTATGATGCAGCTAGTCGGAGGAAGGCTAGACTCTCTGACTTTATTGACCCAGATGGAGAATGGCTTTGGCCTCGAGTTTCTTTGGAATTAATTGACCTATGGGAGAGGGTTCAGGAGGTCAGTCCGTGTCTTAGTGTTAGTGATAGTTGGGTATGGGTTCCTGGTCGTCGGGGTGGTTTCTCTATTGCAAGTGCATGGGAAGCTGTTCGTCCTAGGGGTGGTCGGGTTCTATGGGATGGTTTATTGTGGGGTGGGGGAAATATCCAAAAACATTTCTTCTGTGCGTGGTTGGCTATTAAAGATAGGTTGGGTACTATAGATAGATTGCATAGGTGGGATAGTTCAGTTCCGATGTTGTGCATTCTATGTCGGGGGTGTGGAGTCTCGCGATCACTTATTCTTTTCGTGTTCGTTTGGGGGGGGGGGGGATGTTTGGTCTAGGGTTCTTCGGATCATGGGTTCCTCTCATAGGATTGGGCATTGGAGGGTTGAGTTGTCTTGGATTTGTCATTAA

mRNA sequence

ATGATTTTTTGTGCTGCTAATGAGCCCTCTATCAGATTTATCCGAGAATGTCTTCAGAAGTTTGGTGAGCTTTCAGGTTTGTTCGCAAATCCTAGGAAAAGCTCTATTTTTGTTGCAGGAGTTAATAATGAGAATGCTTCTCATCTGGCTACTTGTATGGGTTTTGTCCGTGGTAATCTCTCTGTTCGTTATCTTGGCCTTCCTCTTTTGGCGGGTCGATTACGTTCTAATGATTATGCTCCTCTGATTCAGCGTATCACTAGCAGGATTCGTTCTTGGACCGCTCGAGTTCTTTCGTTTGCAGGTAGACTGCAGCTTGTTCATTCTGTGCTTCGTAGTTTTCAAGTGTACTGGGCTAGTGTGTTTGTTCTTCCTGCGTATGTGCACAATGAGGTGGACAAGATTCTCAGATCTTATCTTTGGAGGGGTAAGGAGGAAGGTAGAGGAGGTATTAAGGTGGCTTGGGTGGATGTGTTGGGTAAATCTTGGTGTCTTCGGGCGATCTTACGTAAGCGAGAGAAGCTGAAGCATCTTGTAAGGATGAAGGTGGGAAATGGTAATAGTTTTAGAGTTTGGCTTGATCCGTGGTTGCCGGAGGGTGCCATTTTGGAGCAGGTTGGGGAGAGAGTGATGTATGATGCAGCTAGTCGGAGGAAGGCTAGACTCTCTGACTTTATTGACCCAGATGGAGAATGGCTTTGGCCTCGAGTTTCTTTGGAATTAATTGACCTATGGGAGAGGGTTCAGGAGGTCAGTCCGTGTCTTAGTGTTAGTGATAGTTGGGTATGGGTTCCTGGTCGTCGGGGTGGTTTCTCTATTGCAAGTGCATGGGAAGCTGTTCGTCCTAGGGGTGGTCGGGTTCTATGGGATGGTTTATTGTGGGGTGGGGGAAATATCCAAAAACATTTCTTCTGTGCGTGGTTGGCTATTAAAGATAGGTTGGGTACTATAGATAGATTGCATAGGTGGGATAGTTCATCTCGCGATCACTTATTCTTTTCGTGTTCGTTTGGGGGGGGGGGGGATGTTTGGTCTAGGGTTCTTCGGATCATGGGTTCCTCTCATAGGATTGGGCATTGGAGGGTTGAGTTGTCTTGGATTTGTCATTAA

Coding sequence (CDS)

ATGATTTTTTGTGCTGCTAATGAGCCCTCTATCAGATTTATCCGAGAATGTCTTCAGAAGTTTGGTGAGCTTTCAGGTTTGTTCGCAAATCCTAGGAAAAGCTCTATTTTTGTTGCAGGAGTTAATAATGAGAATGCTTCTCATCTGGCTACTTGTATGGGTTTTGTCCGTGGTAATCTCTCTGTTCGTTATCTTGGCCTTCCTCTTTTGGCGGGTCGATTACGTTCTAATGATTATGCTCCTCTGATTCAGCGTATCACTAGCAGGATTCGTTCTTGGACCGCTCGAGTTCTTTCGTTTGCAGGTAGACTGCAGCTTGTTCATTCTGTGCTTCGTAGTTTTCAAGTGTACTGGGCTAGTGTGTTTGTTCTTCCTGCGTATGTGCACAATGAGGTGGACAAGATTCTCAGATCTTATCTTTGGAGGGGTAAGGAGGAAGGTAGAGGAGGTATTAAGGTGGCTTGGGTGGATGTGTTGGGTAAATCTTGGTGTCTTCGGGCGATCTTACGTAAGCGAGAGAAGCTGAAGCATCTTGTAAGGATGAAGGTGGGAAATGGTAATAGTTTTAGAGTTTGGCTTGATCCGTGGTTGCCGGAGGGTGCCATTTTGGAGCAGGTTGGGGAGAGAGTGATGTATGATGCAGCTAGTCGGAGGAAGGCTAGACTCTCTGACTTTATTGACCCAGATGGAGAATGGCTTTGGCCTCGAGTTTCTTTGGAATTAATTGACCTATGGGAGAGGGTTCAGGAGGTCAGTCCGTGTCTTAGTGTTAGTGATAGTTGGGTATGGGTTCCTGGTCGTCGGGGTGGTTTCTCTATTGCAAGTGCATGGGAAGCTGTTCGTCCTAGGGGTGGTCGGGTTCTATGGGATGGTTTATTGTGGGGTGGGGGAAATATCCAAAAACATTTCTTCTGTGCGTGGTTGGCTATTAAAGATAGGTTGGGTACTATAGATAGATTGCATAGGTGGGATAGTTCATCTCGCGATCACTTATTCTTTTCGTGTTCGTTTGGGGGGGGGGGGGATGTTTGGTCTAGGGTTCTTCGGATCATGGGTTCCTCTCATAGGATTGGGCATTGGAGGGTTGAGTTGTCTTGGATTTGTCATTAA

Protein sequence

MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNLSVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWASVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDVLGKSWCLRAILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRWDSSSRDHLFFSCSFGGGGDVWSRVLRIMGSSHRIGHWRVELSWICH
Homology
BLAST of IVF0005421 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.0e-10
Identity = 31/92 (33.70%), Postives = 53/92 (57.61%), Query Frame = 0

Query: 67  LPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWASVFVLPA 126
           +P+L  R+  + +  +++R++SR+  W  + LSFAGRL L  +VL S  V+  S  +LP 
Sbjct: 1   MPVLQKRINKDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQ 60

Query: 127 YVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV 159
            + N +D++ R++LW    E +    V W  V
Sbjct: 61  SILNRLDQLSRTFLWGSTAEKKKQHLVKWSKV 92

BLAST of IVF0005421 vs. ExPASy TrEMBL
Match: A0A5A7V3Z0 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold154G00690 PE=4 SV=1)

HSP 1 Score: 658.7 bits (1698), Expect = 1.5e-185
Identity = 325/383 (84.86%), Postives = 326/383 (85.12%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL
Sbjct: 452 MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 511

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
           SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS
Sbjct: 512 SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 571

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV---------------------- 180
           VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV                      
Sbjct: 572 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDVCLPFEEGGLGIRDGPSWNIAST 631

Query: 181 -----------------------------------LGKSWCLRAILRKREKLKHLVRMKV 240
                                              +GKSWCLRAILRKREKLKHLVRMKV
Sbjct: 632 LKILWLMLTNSGSLWVAWVEAYILKGRSLWDVDSRVGKSWCLRAILRKREKLKHLVRMKV 691

Query: 241 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 300
           GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID
Sbjct: 692 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 751

Query: 301 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 327
           LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF
Sbjct: 752 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 811

BLAST of IVF0005421 vs. ExPASy TrEMBL
Match: A0A5A7TZS0 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19358G00020 PE=4 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 4.7e-152
Identity = 292/437 (66.82%), Postives = 305/437 (69.79%), Query Frame = 0

Query: 1    MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
            MIFCAA+E SI FIRECLQKFGE SGLFANPRKSSIFV GVNNE ASHLA C+G      
Sbjct: 657  MIFCAADELSISFIRECLQKFGEFSGLFANPRKSSIFVVGVNNEYASHLAACIGC--SWK 716

Query: 61   SVRYLGLPLLA-GRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWA 120
            S   L  P  +   LRS D APLIQRITSRIRSWTARVLSFAGRLQLV SVLRS QVYWA
Sbjct: 717  SPCSLSWPSSSHWWLRSIDCAPLIQRITSRIRSWTARVLSFAGRLQLVRSVLRSLQVYWA 776

Query: 121  SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV--------------------- 180
            SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV                     
Sbjct: 777  SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDVCLPFEEGGLGIRDGPSWNIAN 836

Query: 181  ---------------------------------LGKSWCLRAILRKREKLKHLVRMKVGN 240
                                             +G+SWCLRAILRKREK+KH        
Sbjct: 837  TLKILLTNLGSLWVAWMEAYILKGKSLWDVDSRVGRSWCLRAILRKREKMKH-------- 896

Query: 241  GNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLW 300
                                VGERV+YDAASRR+A+LSDFIDP+GEWLWPRVSLELIDLW
Sbjct: 897  -------------------HVGERVLYDAASRREAKLSDFIDPNGEWLWPRVSLELIDLW 956

Query: 301  ERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFC 360
            ERVQEVSPCLSVSDSWVWVPGR+GGFSIASAWEA+ PRGGRVLWDGLLWGGGNI KH FC
Sbjct: 957  ERVQEVSPCLSVSDSWVWVPGRQGGFSIASAWEAICPRGGRVLWDGLLWGGGNIPKHSFC 1016

Query: 361  AWLAIKDRLGTIDRLHRWDSS-------------SRDHLFFSCSFGGGGDVWSRVLRIMG 370
            AWLAIKDRL T DRLHRWDSS             SRDHLFFSC F  GGDVWSRV +IM 
Sbjct: 1017 AWLAIKDRLDTRDRLHRWDSSIPLSCILCQGGVESRDHLFFSCPF--GGDVWSRVFQIME 1062

BLAST of IVF0005421 vs. ExPASy TrEMBL
Match: A0A5A7TKU4 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00080 PE=4 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.5e-150
Identity = 271/412 (65.78%), Postives = 305/412 (74.03%), Query Frame = 0

Query: 1    MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
            MIFC A+EPS+ F+RE LQKFGEL GL+AN  K SIFVAG  NE AS LA  MGF+ GNL
Sbjct: 704  MIFCVADEPSLSFVRETLQKFGELLGLYANLGKISIFVAGSTNEVASRLAANMGFILGNL 763

Query: 61   SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
             VRYL LPLL GRLR +D APLIQRITSRIRSW ARVLSFAGR QLV SV RS QVYWAS
Sbjct: 764  PVRYLSLPLLTGRLRPSDCAPLIQRITSRIRSWNARVLSFAGRFQLVRSVFRSLQVYWAS 823

Query: 121  VFVLPAYVHNEVDKILRSYLWRGKE----------------EGRGGIKVAWVDV------ 180
            VFVLP+ VHN+VDKILRSYLWR ++                     + VAWV+       
Sbjct: 824  VFVLPSCVHNKVDKILRSYLWRVRDGPSWNITSTLKILWLLTSLSSLWVAWVEAYILKRK 883

Query: 181  --------LGKSWCLRAILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERV 240
                    + +SWCLRAILRKR+ LK+ V ++VG+G+S RVWLDPWL    ILEQVGERV
Sbjct: 884  SLWAVDSGVCRSWCLRAILRKRDSLKYHVWIEVGDGSSCRVWLDPWLQGVPILEQVGERV 943

Query: 241  MYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRRGG 300
            +YDAASRR+ARLS+FI PDGEW WPRVS+ELIDLW+RVQ V PCLSV D WVWVPG +GG
Sbjct: 944  LYDAASRREARLSEFIGPDGEWQWPRVSIELIDLWDRVQAVRPCLSVRDRWVWVPGHQGG 1003

Query: 301  FSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRWDSS---- 360
            FSIASA + +RPRGGRV W GLLWGGGN+ KH FCAWL IK++LGT DRLHRWDSS    
Sbjct: 1004 FSIASALDTIRPRGGRVRWVGLLWGGGNVPKHSFCAWLTIKNKLGTRDRLHRWDSSVPMS 1063

Query: 361  ---------SRDHLFFSCSFGGGGDVWSRVLRIMGSSHRIGHWRVELSWICH 370
                     SRDHLFFSC F  G DVWSRVL++M SSHRI +W VELSWICH
Sbjct: 1064 FILCQGGVESRDHLFFSCPF--GRDVWSRVLQVMASSHRIRYWGVELSWICH 1113

BLAST of IVF0005421 vs. ExPASy TrEMBL
Match: A0A5D3CLU1 (Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00420 PE=4 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 8.6e-146
Identity = 275/390 (70.51%), Postives = 289/390 (74.10%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAA+EPSIRFIRECLQKFGELSGLFANPRKS IFVAGVNNENAS LA CMGFVRGNL
Sbjct: 384 MIFCAADEPSIRFIRECLQKFGELSGLFANPRKSFIFVAGVNNENASQLAACMGFVRGNL 443

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
            VRYLGLPLL GRLRSND AP+IQRITSRIRSW ARVLSFAGRLQLV SVLRS QVYWAS
Sbjct: 444 PVRYLGLPLLTGRLRSNDCAPMIQRITSRIRSWIARVLSFAGRLQLVCSVLRSLQVYWAS 503

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEE---------------GRGGIKVAWVDV------- 180
           VFVLPAYVHNEVDKILRSYLWR                     G + VAWV+        
Sbjct: 504 VFVLPAYVHNEVDKILRSYLWRDGPSWNIASTLKILWLMLTSLGSLWVAWVEAYILKGRS 563

Query: 181 -------LGKSWCLRAILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERVM 240
                  +G+SWCLRAILR                             GAILEQVGERV+
Sbjct: 564 LWDVDSRVGRSWCLRAILR-----------------------------GAILEQVGERVL 623

Query: 241 YDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRRGGF 300
           YDAASRR+ARLSDFI  +GEWLW RV LELI+LW+RVQEVSPCLSVSDSWVWVPGR+GGF
Sbjct: 624 YDAASRREARLSDFIGSNGEWLWSRVFLELINLWDRVQEVSPCLSVSDSWVWVPGRQGGF 683

Query: 301 SIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRWDSS----- 349
           SIASAW+A+RPRGGRVLWDGLLWGG NI KH FCAWLAIKDRLGT DRLHRWDSS     
Sbjct: 684 SIASAWKAIRPRGGRVLWDGLLWGGRNIPKHSFCAWLAIKDRLGTRDRLHRWDSSVPMWC 742

BLAST of IVF0005421 vs. ExPASy TrEMBL
Match: A0A5A7UV01 (F17F8.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold896G00020 PE=4 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.6e-139
Identity = 267/386 (69.17%), Postives = 276/386 (71.50%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAA+EPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLA CMGFVRGNL
Sbjct: 346 MIFCAADEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLAACMGFVRGNL 405

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
            +RYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLV  VLRS QVYWAS
Sbjct: 406 PIRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVRFVLRSLQVYWAS 465

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV---------------------- 180
           VFVLPAYVH+EVDKIL SYLWRGKEEGRGGIKVAWVDV                      
Sbjct: 466 VFVLPAYVHHEVDKILISYLWRGKEEGRGGIKVAWVDVCLPFEESGLGIRDGPSWNIAST 525

Query: 181 -----------------------------------LGKSWCLRAILRKREKLKHLVRMKV 240
                                              +G+SW LRAILRKREKLKHLV MKV
Sbjct: 526 LKILLLMLTNSGSLWVAWVKAYILKGRSLWDVDSRVGRSWRLRAILRKREKLKHLVWMKV 585

Query: 241 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 300
           GN NS RVWLD WL   AILEQVGERVMYDAAS R+ARLSDFIDPDGEWLWP        
Sbjct: 586 GNSNSCRVWLDSWLLGDAILEQVGERVMYDAASWREARLSDFIDPDGEWLWP-------- 645

Query: 301 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 329
                                   RGGFSIASAWEAVRPRGG+VLWDGLLWGGGNI K+ 
Sbjct: 646 ------------------------RGGFSIASAWEAVRPRGGQVLWDGLLWGGGNIPKYS 699

BLAST of IVF0005421 vs. NCBI nr
Match: KAA0062318.1 (uncharacterized protein E6C27_scaffold154G00690 [Cucumis melo var. makuwa])

HSP 1 Score: 654 bits (1686), Expect = 2.44e-227
Identity = 325/383 (84.86%), Postives = 326/383 (85.12%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL
Sbjct: 452 MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 511

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
           SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS
Sbjct: 512 SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 571

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV---------------------- 180
           VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV                      
Sbjct: 572 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDVCLPFEEGGLGIRDGPSWNIAST 631

Query: 181 -----------------------------------LGKSWCLRAILRKREKLKHLVRMKV 240
                                              +GKSWCLRAILRKREKLKHLVRMKV
Sbjct: 632 LKILWLMLTNSGSLWVAWVEAYILKGRSLWDVDSRVGKSWCLRAILRKREKLKHLVRMKV 691

Query: 241 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 300
           GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID
Sbjct: 692 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 751

Query: 301 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 326
           LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF
Sbjct: 752 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 811

BLAST of IVF0005421 vs. NCBI nr
Match: KAA0046851.1 (uncharacterized protein E6C27_scaffold19358G00020 [Cucumis melo var. makuwa])

HSP 1 Score: 543 bits (1398), Expect = 3.77e-181
Identity = 292/437 (66.82%), Postives = 305/437 (69.79%), Query Frame = 0

Query: 1    MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
            MIFCAA+E SI FIRECLQKFGE SGLFANPRKSSIFV GVNNE ASHLA C+G      
Sbjct: 657  MIFCAADELSISFIRECLQKFGEFSGLFANPRKSSIFVVGVNNEYASHLAACIGC--SWK 716

Query: 61   SVRYLGLPLLAGR-LRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWA 120
            S   L  P  +   LRS D APLIQRITSRIRSWTARVLSFAGRLQLV SVLRS QVYWA
Sbjct: 717  SPCSLSWPSSSHWWLRSIDCAPLIQRITSRIRSWTARVLSFAGRLQLVRSVLRSLQVYWA 776

Query: 121  SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV--------------------- 180
            SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV                     
Sbjct: 777  SVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDVCLPFEEGGLGIRDGPSWNIAN 836

Query: 181  ---------------------------------LGKSWCLRAILRKREKLKHLVRMKVGN 240
                                             +G+SWCLRAILRKREK+KH        
Sbjct: 837  TLKILLTNLGSLWVAWMEAYILKGKSLWDVDSRVGRSWCLRAILRKREKMKH-------- 896

Query: 241  GNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLW 300
                                VGERV+YDAASRR+A+LSDFIDP+GEWLWPRVSLELIDLW
Sbjct: 897  -------------------HVGERVLYDAASRREAKLSDFIDPNGEWLWPRVSLELIDLW 956

Query: 301  ERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFC 360
            ERVQEVSPCLSVSDSWVWVPGR+GGFSIASAWEA+ PRGGRVLWDGLLWGGGNI KH FC
Sbjct: 957  ERVQEVSPCLSVSDSWVWVPGRQGGFSIASAWEAICPRGGRVLWDGLLWGGGNIPKHSFC 1016

Query: 361  AWLAIKDRLGTIDRLHRWDSS-------------SRDHLFFSCSFGGGGDVWSRVLRIMG 369
            AWLAIKDRL T DRLHRWDSS             SRDHLFFSC FGG  DVWSRV +IM 
Sbjct: 1017 AWLAIKDRLDTRDRLHRWDSSIPLSCILCQGGVESRDHLFFSCPFGG--DVWSRVFQIME 1062

BLAST of IVF0005421 vs. NCBI nr
Match: TYK12108.1 (non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 522 bits (1344), Expect = 9.74e-178
Identity = 275/390 (70.51%), Postives = 289/390 (74.10%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAA+EPSIRFIRECLQKFGELSGLFANPRKS IFVAGVNNENAS LA CMGFVRGNL
Sbjct: 384 MIFCAADEPSIRFIRECLQKFGELSGLFANPRKSFIFVAGVNNENASQLAACMGFVRGNL 443

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
            VRYLGLPLL GRLRSND AP+IQRITSRIRSW ARVLSFAGRLQLV SVLRS QVYWAS
Sbjct: 444 PVRYLGLPLLTGRLRSNDCAPMIQRITSRIRSWIARVLSFAGRLQLVCSVLRSLQVYWAS 503

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEEGR---------------GGIKVAWVDV------- 180
           VFVLPAYVHNEVDKILRSYLWR                     G + VAWV+        
Sbjct: 504 VFVLPAYVHNEVDKILRSYLWRDGPSWNIASTLKILWLMLTSLGSLWVAWVEAYILKGRS 563

Query: 181 -------LGKSWCLRAILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERVM 240
                  +G+SWCLRAILR                             GAILEQVGERV+
Sbjct: 564 LWDVDSRVGRSWCLRAILR-----------------------------GAILEQVGERVL 623

Query: 241 YDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRRGGF 300
           YDAASRR+ARLSDFI  +GEWLW RV LELI+LW+RVQEVSPCLSVSDSWVWVPGR+GGF
Sbjct: 624 YDAASRREARLSDFIGSNGEWLWSRVFLELINLWDRVQEVSPCLSVSDSWVWVPGRQGGF 683

Query: 301 SIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRWDSS----- 348
           SIASAW+A+RPRGGRVLWDGLLWGG NI KH FCAWLAIKDRLGT DRLHRWDSS     
Sbjct: 684 SIASAWKAIRPRGGRVLWDGLLWGGRNIPKHSFCAWLAIKDRLGTRDRLHRWDSSVPMWC 742

BLAST of IVF0005421 vs. NCBI nr
Match: KAA0042317.1 (non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa] >TYK15441.1 non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 534 bits (1375), Expect = 3.60e-177
Identity = 272/412 (66.02%), Postives = 305/412 (74.03%), Query Frame = 0

Query: 1    MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
            MIFC A+EPS+ F+RE LQKFGEL GL+AN  K SIFVAG  NE AS LA  MGF+ GNL
Sbjct: 704  MIFCVADEPSLSFVRETLQKFGELLGLYANLGKISIFVAGSTNEVASRLAANMGFILGNL 763

Query: 61   SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
             VRYL LPLL GRLR +D APLIQRITSRIRSW ARVLSFAGR QLV SV RS QVYWAS
Sbjct: 764  PVRYLSLPLLTGRLRPSDCAPLIQRITSRIRSWNARVLSFAGRFQLVRSVFRSLQVYWAS 823

Query: 121  VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIK----------------VAWVDV------ 180
            VFVLP+ VHN+VDKILRSYLWR ++     I                 VAWV+       
Sbjct: 824  VFVLPSCVHNKVDKILRSYLWRVRDGPSWNITSTLKILWLLTSLSSLWVAWVEAYILKRK 883

Query: 181  --------LGKSWCLRAILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERV 240
                    + +SWCLRAILRKR+ LK+ V ++VG+G+S RVWLDPWL    ILEQVGERV
Sbjct: 884  SLWAVDSGVCRSWCLRAILRKRDSLKYHVWIEVGDGSSCRVWLDPWLQGVPILEQVGERV 943

Query: 241  MYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERVQEVSPCLSVSDSWVWVPGRRGG 300
            +YDAASRR+ARLS+FI PDGEW WPRVS+ELIDLW+RVQ V PCLSV D WVWVPG +GG
Sbjct: 944  LYDAASRREARLSEFIGPDGEWQWPRVSIELIDLWDRVQAVRPCLSVRDRWVWVPGHQGG 1003

Query: 301  FSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRWDSS---- 360
            FSIASA + +RPRGGRV W GLLWGGGN+ KH FCAWL IK++LGT DRLHRWDSS    
Sbjct: 1004 FSIASALDTIRPRGGRVRWVGLLWGGGNVPKHSFCAWLTIKNKLGTRDRLHRWDSSVPMS 1063

Query: 361  ---------SRDHLFFSCSFGGGGDVWSRVLRIMGSSHRIGHWRVELSWICH 369
                     SRDHLFFSC FG   DVWSRVL++M SSHRI +W VELSWICH
Sbjct: 1064 FILCQGGVESRDHLFFSCPFGR--DVWSRVLQVMASSHRIRYWGVELSWICH 1113

BLAST of IVF0005421 vs. NCBI nr
Match: KAA0059752.1 (F17F8.5 [Cucumis melo var. makuwa] >TYJ99767.1 F17F8.5 [Cucumis melo var. makuwa])

HSP 1 Score: 502 bits (1293), Expect = 7.22e-170
Identity = 267/386 (69.17%), Postives = 276/386 (71.50%), Query Frame = 0

Query: 1   MIFCAANEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLATCMGFVRGNL 60
           MIFCAA+EPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLA CMGFVRGNL
Sbjct: 346 MIFCAADEPSIRFIRECLQKFGELSGLFANPRKSSIFVAGVNNENASHLAACMGFVRGNL 405

Query: 61  SVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVHSVLRSFQVYWAS 120
            +RYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLV  VLRS QVYWAS
Sbjct: 406 PIRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARVLSFAGRLQLVRFVLRSLQVYWAS 465

Query: 121 VFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVDV---------------------- 180
           VFVLPAYVH+EVDKIL SYLWRGKEEGRGGIKVAWVDV                      
Sbjct: 466 VFVLPAYVHHEVDKILISYLWRGKEEGRGGIKVAWVDVCLPFEESGLGIRDGPSWNIAST 525

Query: 181 -----------------------------------LGKSWCLRAILRKREKLKHLVRMKV 240
                                              +G+SW LRAILRKREKLKHLV MKV
Sbjct: 526 LKILLLMLTNSGSLWVAWVKAYILKGRSLWDVDSRVGRSWRLRAILRKREKLKHLVWMKV 585

Query: 241 GNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELID 300
           GN NS RVWLD WL   AILEQVGERVMYDAAS R+ARLSDFIDPDGEWLWPR       
Sbjct: 586 GNSNSCRVWLDSWLLGDAILEQVGERVMYDAASWREARLSDFIDPDGEWLWPR------- 645

Query: 301 LWERVQEVSPCLSVSDSWVWVPGRRGGFSIASAWEAVRPRGGRVLWDGLLWGGGNIQKHF 328
                                    GGFSIASAWEAVRPRGG+VLWDGLLWGGGNI K+ 
Sbjct: 646 -------------------------GGFSIASAWEAVRPRGGQVLWDGLLWGGGNIPKYS 699

BLAST of IVF0005421 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-39
Identity = 111/351 (31.62%), Postives = 158/351 (45.01%), Query Frame = 0

Query: 38  VAGVNNENASHLATCMGFVRGNLSVRYLGLPLLAGRLRSNDYAPLIQRITSRIRSWTARV 97
           +AGV + + + +     F  G L VRYLGLPLL  ++ ++DY PL+++I  RI  WTAR 
Sbjct: 1   MAGVKDNDKADILHSFPFASGALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARH 60

Query: 98  LSFAGRLQLVHSVLRSFQVYWASVFVLPAYVHNEVDKILRSYLWRGKEEGRGGIKVAWVD 157
           LSFAGRLQL+ SV+ S   +W S F LP+    E+D I  S+LW G E      KVAW D
Sbjct: 61  LSFAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSD 120

Query: 158 V--------LG---------------------KSWCLRAILRKREKLKHLVRMKVGNGNS 217
           V        LG                      SW  + IL+ R      V+  + NG++
Sbjct: 121 VCTPKDEGGLGIRSLKEANKGSFWSISGNTTLGSWMWKKILKHRALASGFVKHDIHNGSN 180

Query: 218 FRVWLDPWLPEGAILEQVGERVMYDAASRRKARLSDFIDPDGEWLWPRVSLELIDLWERV 277
              W D W   G +++  G R   D      A +++ +      +  R      D   R+
Sbjct: 181 TSFWFDNWSKIGRLIDVTGHRGCIDMGITLHASVAEAV------VNHRPRRHRHDTLLRI 240

Query: 278 QEV------SPCLSVSDSWVWVPGR---RGGFSIASAWEAVRPRGGRVLWDGLLWGGGNI 337
           ++V          S  D+  W       +  F+    W A R    +V W   +W     
Sbjct: 241 EDVIAEVRHQGLTSGEDTVRWKGNGDIFKPCFNTKETWAATREPKLKVNWYKGVWFSHAT 300

BLAST of IVF0005421 vs. TAIR 10
Match: AT1G60720.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 91.7 bits (226), Expect = 1.4e-18
Identity = 66/223 (29.60%), Postives = 97/223 (43.50%), Query Frame = 0

Query: 168 ILRKREKLKHLVRMKVGNGNSFRVWLDPWLPEGAILEQVGERVMYDAASRR---KARLSD 227
           +L  R   +  V+  +GNG     W D W   G +++ +G+   Y + S R    AR+ +
Sbjct: 2   LLFLRPLAEQFVKCNLGNGRIAHFWHDSWTSLGPLIKVMGD---YGSRSLRIPLNARVVE 61

Query: 228 FIDPDGEWLWP----RVSLELIDLWERVQEVSPCLSVSDSWVWVPGR--RGGFSIASAWE 287
            +  +G W  P      +  + D    +   SP  ++ DS+ WV G     GFS A  W+
Sbjct: 62  ALGVNG-WKLPLSRSAPAQAIHDHISTITTPSPA-TIEDSFDWVVGGVVCQGFSSARTWD 121

Query: 288 AVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRW-------------DS 347
           A+RPR   + W   +W  G + KH F  W++  DRL T  RL  W             ++
Sbjct: 122 AIRPRAPELDWAKAVWFKGAVPKHAFNMWISQLDRLPTRQRLASWGHIQSFDCCLCTIET 181

Query: 348 SSRDHLFFSCSFGGGGDVWSRVL-RIMGSSHRIGHWRVELSWI 368
            SRDHL FSC F     VW     R+         W   LSW+
Sbjct: 182 ESRDHLLFSCEF--AAQVWRLAFSRLCPRQRLFCSWAELLSWM 217

BLAST of IVF0005421 vs. TAIR 10
Match: AT2G02520.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 55.1 bits (131), Expect = 1.4e-07
Identity = 29/83 (34.94%), Postives = 38/83 (45.78%), Query Frame = 0

Query: 275 SAWEAVRPRGGRVLWDGLLWGGGNIQKHFFCAWLAIKDRLGTIDRLHRW----------- 334
           + W  + P G RV W   +W  G I KH F AW+ ++ RL T DR+  W           
Sbjct: 29  ATWLHLNPIGERVDWFKAIWFKGKIPKHAFIAWVNMRHRLHTKDRMISWGFIFPPLCLFC 88

Query: 335 --DSSSRDHLFFSCSFGGGGDVW 345
                +R HLFF C F    +VW
Sbjct: 89  NTHDETRQHLFFDCEF--AREVW 109

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P0C2F63.0e-1033.70Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A5A7V3Z01.5e-18584.86Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A5A7TZS04.7e-15266.82Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A5A7TKU47.5e-15065.78Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak... [more]
A0A5D3CLU18.6e-14670.51Non-LTR retroelement reverse transcriptase-like protein OS=Cucumis melo var. mak... [more]
A0A5A7UV011.6e-13969.17F17F8.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold896G00020 PE=4 S... [more]
Match NameE-valueIdentityDescription
KAA0062318.12.44e-22784.86uncharacterized protein E6C27_scaffold154G00690 [Cucumis melo var. makuwa][more]
KAA0046851.13.77e-18166.82uncharacterized protein E6C27_scaffold19358G00020 [Cucumis melo var. makuwa][more]
TYK12108.19.74e-17870.51non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuw... [more]
KAA0042317.13.60e-17766.02non-LTR retroelement reverse transcriptase-like protein [Cucumis melo var. makuw... [more]
KAA0059752.17.22e-17069.17F17F8.5 [Cucumis melo var. makuwa] >TYJ99767.1 F17F8.5 [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
AT3G24255.11.4e-3931.62RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT1G60720.11.4e-1829.60RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT2G02520.11.4e-0734.94RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 271..328
e-value: 2.4E-7
score: 31.3
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 13..294

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0005421.1IVF0005421.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane