IVF0004705 (gene) Melon (IVF77) v1

Overview
NameIVF0004705
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionAMP-dependent synthetase and ligase family protein
Locationchr07: 392532 .. 394660 (+)
RNA-Seq ExpressionIVF0004705
SyntenyIVF0004705
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAATCATCCCTTGCTCTGCCAATTCCCTTCCTCTCACTCCCATCACTTTCTTGGAGCGAGCCGCCGCCGTCTTTGCTGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGGGAGAAACTCTCCAACGATGTACTAAATTCGCTTCTGCTCTAGTTCACCTCGGAATCTCTCGCGGCGATGTGGTCTGTATTTTGAACTTTTTCATTTCGTTTGTTTGATATATTTTTGGTTTTTGGTTTGGCTGTTGATTGGCATTGTTAGTATCTGGATGTGGCTGTATTTGTAGAACCGGAGTGATTTGCTGCATTACTGTTAATGAAAATTTATATTGAATTGGAGATAGGTGAGAACGGTGAATTTGAGCTTTGATAGCGTGCATTGTGTATGACTGAAGATGTAGGTTTCGTTTTGAAGAAGAATCACTAAGTTGCTGATTTTGTTCTTTCTTGCTTCTGAATTGTGAATCAATTCGATTCTGTTTCTTATGGTGTTGATTCTTCTTAGTATCTAATTTTATGTTACGGATCTAATTTGAAGCTTCGATGTTCTAATTGTTTTCCGGATCATTCGGTTGGTTGTTGATTATAATCATTGATCTAGTTTTGCAACTAATTACTCGAATCAACTCGAGCAGGTTGCTGTTTTGGCACCGAATGTTCCAGCTACATACGAGCTTCATTTTGCTGTACCAATGGCTGGTGCAATTCTTTGCACTCTCAACATGCGCCATGATGCAGCAATGATTTCTACATTGTTGGGTCATTCAGAAGCTAAAATCATTGTTGTAGACCACCAATATCTACATATTGTAAAGGCAGCAATCGAAATTATGTCCAAGATGACGGGGGCAGAAGAGCTGCCTCGTATTGTCATTGTTCAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGACTCTGCTTCAGATGATTTAGAGTACGAGAGCCTTCTAAACACTGGAACACTCGATTTCGAAATCAGACGTCCTATAGATGAATGGGATCCAATCTCTCTTAACTATACTTCAGGCACAACATCAAGACCAAAAGGTGTGATTTACTCCCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCGATGCCTGTATATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTATGACTTGGGGTGTGGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGAATGCCAAAGAAATCTTCGCCAATATTTCTCTACACAAGGTCACTCACATGGGTGGCGCACCGACCGTATTAAACATGATCGTCAATGCACCAATTACTGAACAGAAGCCCCTTCCGGGGAAGGTAACTGTAATGACTGGGGGTGCTCCTCCGCCATCTAACATACTGTACAAGATGAGAGAATTAGGATTCCTTATTGTCCATTGTTATGGTTTAACCGAAACATGTGGTGTTGCAACATTTTGCCAATGGAAACCTGAATGGGATTCCCTTCCCGATGACAAGCAAGCAAAACTGAAATCTCGTCAAGGAATGCAACATATCGGAGTGGAGGGATTGGATATTAAGAATCCCATCACCATGGAAAGTGTTCCAGCTGATGGAAAAACCATGGGGGAAGTTATGATCAGAGGTAACACCGTAATGAGTGGTTATTTGAAAGATCTTAAAGCTACAGAAGAAGCCTTCAATGGGGGATGGTTTCGAAGTGGAGATTTGGGAGTAAGACATCCCGATGGTTACATAGAACTGAAAGATCGTTCAAAAGACATCATAATTTCTGGAGGAGAAAACATTAGTACAATTGAAGTTGAATCTGTGCTATTCAATCATCCATCAGTTCTTGAAGCTGCCATTGTTGGAAGACCTGATGATCATTGGGGTGAAACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTCTAATGCTACACAAGAAGAAATCATAGAGTTTTGTAGAGATAAACTTCCTCATTACATGGCTCCAAAAACTGTTGTGTTTAAATCAGATTTGCCTAAATCTTCTACAGGGAAAATTCAGAAGCTTATTCTCAAGGAAGAAGCTAAGGCCATGGGGAGCCTTTCCCCTGCTAAGAGGTTTGACTAA

mRNA sequence

ATGGAAGGAATCATCCCTTGCTCTGCCAATTCCCTTCCTCTCACTCCCATCACTTTCTTGGAGCGAGCCGCCGCCGTCTTTGCTGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGGGAGAAACTCTCCAACGATGTACTAAATTCGCTTCTGCTCTAGTTCACCTCGGAATCTCTCGCGGCGATGTGGTTGCTGTTTTGGCACCGAATGTTCCAGCTACATACGAGCTTCATTTTGCTGTACCAATGGCTGGTGCAATTCTTTGCACTCTCAACATGCGCCATGATGCAGCAATGATTTCTACATTGTTGGGTCATTCAGAAGCTAAAATCATTGTTGTAGACCACCAATATCTACATATTGTAAAGGCAGCAATCGAAATTATGTCCAAGATGACGGGGGCAGAAGAGCTGCCTCGTATTGTCATTGTTCAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGACTCTGCTTCAGATGATTTAGAGTACGAGAGCCTTCTAAACACTGGAACACTCGATTTCGAAATCAGACGTCCTATAGATGAATGGGATCCAATCTCTCTTAACTATACTTCAGGCACAACATCAAGACCAAAAGGTGTGATTTACTCCCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCGATGCCTGTATATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTATGACTTGGGGTGTGGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGAATGCCAAAGAAATCTTCGCCAATATTTCTCTACACAAGGTCACTCACATGGGTGGCGCACCGACCGTATTAAACATGATCGTCAATGCACCAATTACTGAACAGAAGCCCCTTCCGGGGAAGGTAACTGTAATGACTGGGGGTGCTCCTCCGCCATCTAACATACTGTACAAGATGAGAGAATTAGGATTCCTTATTGTCCATTGTTATGGTTTAACCGAAACATGTGGTGTTGCAACATTTTGCCAATGGAAACCTGAATGGGATTCCCTTCCCGATGACAAGCAAGCAAAACTGAAATCTCGTCAAGGAATGCAACATATCGGAGTGGAGGGATTGGATATTAAGAATCCCATCACCATGGAAAGTGTTCCAGCTGATGGAAAAACCATGGGGGAAGTTATGATCAGAGGTAACACCGTAATGAGTGGTTATTTGAAAGATCTTAAAGCTACAGAAGAAGCCTTCAATGGGGGATGGTTTCGAAGTGGAGATTTGGGAGTAAGACATCCCGATGGTTACATAGAACTGAAAGATCGTTCAAAAGACATCATAATTTCTGGAGGAGAAAACATTAGTACAATTGAAGTTGAATCTGTGCTATTCAATCATCCATCAGTTCTTGAAGCTGCCATTGTTGGAAGACCTGATGATCATTGGGGTGAAACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTCTAATGCTACACAAGAAGAAATCATAGAGTTTTGTAGAGATAAACTTCCTCATTACATGGCTCCAAAAACTGTTGTGTTTAAATCAGATTTGCCTAAATCTTCTACAGGGAAAATTCAGAAGCTTATTCTCAAGGAAGAAGCTAAGGCCATGGGGAGCCTTTCCCCTGCTAAGAGGTTTGACTAA

Coding sequence (CDS)

ATGGAAGGAATCATCCCTTGCTCTGCCAATTCCCTTCCTCTCACTCCCATCACTTTCTTGGAGCGAGCCGCCGCCGTCTTTGCTGACAGAATTTCTCTCGTCTATGGAAATGTCCGCTTCACTTGGGGAGAAACTCTCCAACGATGTACTAAATTCGCTTCTGCTCTAGTTCACCTCGGAATCTCTCGCGGCGATGTGGTTGCTGTTTTGGCACCGAATGTTCCAGCTACATACGAGCTTCATTTTGCTGTACCAATGGCTGGTGCAATTCTTTGCACTCTCAACATGCGCCATGATGCAGCAATGATTTCTACATTGTTGGGTCATTCAGAAGCTAAAATCATTGTTGTAGACCACCAATATCTACATATTGTAAAGGCAGCAATCGAAATTATGTCCAAGATGACGGGGGCAGAAGAGCTGCCTCGTATTGTCATTGTTCAAGAGTTTGATCATCCATCCTCCAACATCAACGGATTCGACTCTGCTTCAGATGATTTAGAGTACGAGAGCCTTCTAAACACTGGAACACTCGATTTCGAAATCAGACGTCCTATAGATGAATGGGATCCAATCTCTCTTAACTATACTTCAGGCACAACATCAAGACCAAAAGGTGTGATTTACTCCCATAGAGGTACTTATCTCAATACTCTATCCACAATCCTTCTGAATGATATGTCCTCGATGCCTGTATATTTGTGGACTGTTCCAATGTTCCATTGCAACGGATGGTCTATGACTTGGGGTGTGGCTGCACAAGGCGGCACAAACATCTGCCAAAGAAATGTGAATGCCAAAGAAATCTTCGCCAATATTTCTCTACACAAGGTCACTCACATGGGTGGCGCACCGACCGTATTAAACATGATCGTCAATGCACCAATTACTGAACAGAAGCCCCTTCCGGGGAAGGTAACTGTAATGACTGGGGGTGCTCCTCCGCCATCTAACATACTGTACAAGATGAGAGAATTAGGATTCCTTATTGTCCATTGTTATGGTTTAACCGAAACATGTGGTGTTGCAACATTTTGCCAATGGAAACCTGAATGGGATTCCCTTCCCGATGACAAGCAAGCAAAACTGAAATCTCGTCAAGGAATGCAACATATCGGAGTGGAGGGATTGGATATTAAGAATCCCATCACCATGGAAAGTGTTCCAGCTGATGGAAAAACCATGGGGGAAGTTATGATCAGAGGTAACACCGTAATGAGTGGTTATTTGAAAGATCTTAAAGCTACAGAAGAAGCCTTCAATGGGGGATGGTTTCGAAGTGGAGATTTGGGAGTAAGACATCCCGATGGTTACATAGAACTGAAAGATCGTTCAAAAGACATCATAATTTCTGGAGGAGAAAACATTAGTACAATTGAAGTTGAATCTGTGCTATTCAATCATCCATCAGTTCTTGAAGCTGCCATTGTTGGAAGACCTGATGATCATTGGGGTGAAACTCCATGTGCTTTTGTGAATCTCAAAGATGGTTCTAATGCTACACAAGAAGAAATCATAGAGTTTTGTAGAGATAAACTTCCTCATTACATGGCTCCAAAAACTGTTGTGTTTAAATCAGATTTGCCTAAATCTTCTACAGGGAAAATTCAGAAGCTTATTCTCAAGGAAGAAGCTAAGGCCATGGGGAGCCTTTCCCCTGCTAAGAGGTTTGACTAA

Protein sequence

MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLGISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQYLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEAKAMGSLSPAKRFD
Homology
BLAST of IVF0004705 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 8.0e-217
Identity = 353/556 (63.49%), Postives = 448/556 (80.58%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEG I   AN +PLTPI+FL+R+A V+ADR+S+VYG+V++TW +T  RC + ASAL  LG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           IS GDVV+VLAPNVPA  ELHF VPMAGA+LCTLN+RHD+++++ LL HS  K+I  DHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L I + A EI+S     +++P +V++ E    + +++    + + +EYE ++  G  DF
Sbjct: 123 FLQIAEGACEILSNK--GDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDF 182

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           E+ RP DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMF
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMF 242

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW + WGV A GGTNIC RNV AK IF NIS HKVTHMGGAPT+LNMI+NAP +EQK
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKV+ +TG APPP+++++KM ELGF + H YGLTET G  T C WKPEWDSLP ++Q
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AK+K+RQG+ H+G+E + +K+P+TM ++PADG TMGEV+ RGNTVM+GYLK+ +AT+EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVES LF HP VLEAA+V RP
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           D++WGET CAFV LKDGS A+ EE+I +CRD+LPHYMAP+++VF+ DLPK+STGK+QK +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFE-DLPKTSTGKVQKFV 542

Query: 541 LKEEAKAMGSLSPAKR 557
           L+ +AKA+ SLS   R
Sbjct: 543 LRTKAKALVSLSKKGR 553

BLAST of IVF0004705 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 733.0 bits (1891), Expect = 2.5e-210
Identity = 353/557 (63.38%), Postives = 436/557 (78.28%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           +EG++   AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T QRC + ASAL +LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVA LAPNVPA +ELHFAVPMAG ILC LN R D + +S LL HSEAKI+ VDHQ
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEF----DHPSSNINGFDSA-SDDLEYESLLNT 180
            L I   A+++++K     +  ++V++ +     D    + + F S  S D EYE+LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GTLDFEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +FEI +P  EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAP 300
           TVPMFHCNGW + WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMIVN  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 ITEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSL 360
           +TE KPLP +V +MTGG+PP   IL KM ELGF + H YGLTET G  T C WKPEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKA 420
             +++ KLK+RQG+QH+G+EGLD+K+P+TME+VP DG TMGEVM RGNTVMSGY KD++A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TEEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAA 480
           T +AF G WF SGDL V++PDGYIE+KDR KD+IISGGENIS++EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGSNATQ-EEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTG 540
           +V RPD HWG+TPC FV LK+G +  + EEII FCRD LPHYMAPKT+VF  D+PK+STG
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF-GDIPKTSTG 587

Query: 541 KIQKLILKEEAKAMGSL 552
           K+QK +L+++A  MGSL
Sbjct: 588 KVQKYLLRKKADEMGSL 603

BLAST of IVF0004705 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 714.1 bits (1842), Expect = 1.2e-204
Identity = 348/556 (62.59%), Postives = 435/556 (78.24%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASAL-VHL 60
           MEG++ CSAN +PL+PITFLER++  + D  SLVYG+VR+TW +T  RC K ASAL  HL
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  GISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDH 120
           GIS GDVVA  + N+P  YELHFAVPMAG ILCTLN R+D+AM+STLL HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QYLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLD 180
           Q L   +AA++++++     + P +V++ + +        F S+S D  Y  LL  G+ D
Sbjct: 149 QLLETARAALDLLAQKD--IKPPTLVLLTDSE-------SFTSSSYD-HYNHLLANGSDD 208

Query: 181 FEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPM 240
           FEIRRP +EWDPIS+NYTSGTT+RPK V+YSHRG YLN+++T+LL+ M +  VYLW+VPM
Sbjct: 209 FEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPM 268

Query: 241 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITE- 300
           FHCNGW   WG AAQG TNIC R V+ K IF NI LHKVTH G APTVLNMIVN+P    
Sbjct: 269 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 328

Query: 301 QKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDD 360
             PLP KV VMTGG+PPP  ++ +M E+GF + H YGLTETCG A  C  KPEWD+L  +
Sbjct: 329 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPE 388

Query: 361 KQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEE 420
           ++  LK+RQG+ H+ +E +D+++P+TMESV ADG T+GEVM RGNTVMSGY KDLKATEE
Sbjct: 389 ERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEE 448

Query: 421 AFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVG 480
           AF GGWFRSGDLGV+H DGYI+LKDR KD++ISGGENIST+EVE+VL++H +VLEAA+V 
Sbjct: 449 AFEGGWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVA 508

Query: 481 RPDDHWGETPCAFVNLKDG--SNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKI 540
           RPD  WGETPCAFV LK+G  ++ + ++II+FCRD+LPHYMAPKTVVF+ +LPK+STGKI
Sbjct: 509 RPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFE-ELPKTSTGKI 568

Query: 541 QKLILKEEAKAMGSLS 553
           QK ILKE+A AMGSLS
Sbjct: 569 QKYILKEKAMAMGSLS 573

BLAST of IVF0004705 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 9.5e-202
Identity = 344/556 (61.87%), Postives = 433/556 (77.88%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASAL-VHL 60
           MEG++ CSAN +PL+PITFLER++  + D  SLVYG+VR+TW +T  RC K ASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  GISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDH 120
           GIS GDVVA  + N+P  YELHFAVPMAG ILCTLN R+D+AM+STLL HSEAK+I V+ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QYLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLD 180
           Q L   +AA++++++     + P +V++ + +        F S+S D  Y  LL  G+ D
Sbjct: 149 QLLETARAALDLLAQKD--IKPPTLVLLTDSE-------SFTSSSYD-HYNHLLANGSDD 208

Query: 181 FEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPM 240
           FEIRRP +E DPIS+NYTSGTT+RPK V+YSHRG YLN+++T+LL+ M +  VYLW+VPM
Sbjct: 209 FEIRRPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPM 268

Query: 241 FHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITE- 300
           FHCNGW   WG AAQG TNIC R V+ K IF NI LHKVTH G APTVLNMIVN+P    
Sbjct: 269 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 328

Query: 301 QKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDD 360
             PLP KV VMTGG+PPP  ++ +M E+GF + H YGLTET G AT C  KPEWD+L  +
Sbjct: 329 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPE 388

Query: 361 KQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEE 420
           ++  LK+RQG+ H+ +E +D+++P++MESV ADG T+GEVM RGNTVMSGY KDLKATEE
Sbjct: 389 ERYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEE 448

Query: 421 AFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVG 480
           AF GGWFR+GDLGV+H DGYI+LKDR KD++ISGGEN+ST+EVE+VL++H +VLEAA+V 
Sbjct: 449 AFEGGWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVA 508

Query: 481 RPDDHWGETPCAFVNLKDG--SNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKI 540
           RPD  WGETPCAFV LK+G  +  + ++II+FCRD+LPHYMAPKTVVF+ +LPK+STGKI
Sbjct: 509 RPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFE-ELPKTSTGKI 568

Query: 541 QKLILKEEAKAMGSLS 553
           QK ILKE+AKAMGSLS
Sbjct: 569 QKYILKEKAKAMGSLS 573

BLAST of IVF0004705 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 597.8 bits (1540), Expect = 1.2e-169
Identity = 282/564 (50.00%), Postives = 395/564 (70.04%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           M+ +  C AN +PLTP+TFL RA   +A+R S++Y   RFTW +T +RC + AS+L  L 
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           I + DVV+VLAPNVPATYE+HFAVPMAGA+L T+N R D   I+ +L HSEAK++ VD++
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YLHIVKAAIEIM-------SKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLL 180
           YL   + A+E++       ++ +    +P+++++ +   P+          D LEYE L+
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTR-----IQQQDQLEYEQLV 180

Query: 181 NTGTLDFEIRRPI-DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPV 240
           + G  ++     + DEWDPI LNYTSGTTS PKGV+YSHRG +L+TL+TI+  +M + PV
Sbjct: 181 HQGNPEYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPV 240

Query: 241 YLWTVPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIV 300
           YLW++PMFH NGW++TWG+AA+GGTN+C RN  A+EI++NI+LHKVTHM  APTV N+++
Sbjct: 241 YLWSLPMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILL 300

Query: 301 NAPITEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEW 360
            A   E++ +   V VM GGAPPP+ ++ K+ ELGF +VHCYG+TE  G    C+W+ EW
Sbjct: 301 EAKPHERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEW 360

Query: 361 DSLPDDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKD 420
           + L  + QA LK+RQG+  + +E +D+KN  TM+SVP +GKTMGE+ +RG+++M GY K+
Sbjct: 361 NKLSREDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKN 420

Query: 421 LKATEEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVL 480
            KA  + F  GWF +GD+ V H DGY+E+KDR KDIIISGGENIS+IEVE+ +  HPSV+
Sbjct: 421 DKANSQVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVI 480

Query: 481 EAAIVGRPDDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSS 540
           EAA+V  P   WGETPCAFV         + +II  C+ +LP +M PK V F  +LPK+ 
Sbjct: 481 EAAVVAMPHPRWGETPCAFVIKTKNPEIKEADIIVHCKKELPGFMVPKHVQFLEELPKTG 540

Query: 541 TGKIQKLILKEEAKAMGSLSPAKR 557
           TGK++KL L+E AK+ G    A +
Sbjct: 541 TGKVKKLQLREMAKSFGIFDNANQ 559

BLAST of IVF0004705 vs. ExPASy TrEMBL
Match: A0A5D3D8V2 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G00710 PE=4 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPAKRFD 559
           LKEEAKAMGSLSPAKRFD
Sbjct: 541 LKEEAKAMGSLSPAKRFD 558

BLAST of IVF0004705 vs. ExPASy TrEMBL
Match: A0A1S3BV31 (probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103493806 PE=4 SV=1)

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPAKR 557
           LKEEAKAMGSLSPAKR
Sbjct: 541 LKEEAKAMGSLSPAKR 556

BLAST of IVF0004705 vs. ExPASy TrEMBL
Match: A0A5A7VBM0 (Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G003580 PE=4 SV=1)

HSP 1 Score: 1117.4 bits (2889), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAK 547
           LKEEAK
Sbjct: 541 LKEEAK 546

BLAST of IVF0004705 vs. ExPASy TrEMBL
Match: A0A0A0L3B4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1)

HSP 1 Score: 1067.4 bits (2759), Expect = 2.1e-308
Identity = 526/554 (94.95%), Postives = 536/554 (96.75%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEG I CSANS+PLTPITFLERAAAVF DRISLVYGNVRFTW ETLQRCTKFASALVH+G
Sbjct: 1   MEG-IRCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAM+STLLGHSEAKIIV DHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSK   AEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLH VTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PL GKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTET GVATFC WKPEWDSLPD+KQ
Sbjct: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEG+DIKNPITMESVPADGKTMGEVMIRGNTVM GYLKD+KATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK GSNAT+EEII+FCRD LPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPA 555
           LKEEAKAMG+LS A
Sbjct: 541 LKEEAKAMGTLSLA 553

BLAST of IVF0004705 vs. ExPASy TrEMBL
Match: A0A6J1C834 (probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN=LOC111009236 PE=4 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.0e-267
Identity = 445/555 (80.18%), Postives = 499/555 (89.91%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEG+  CSAN + LTPITFLER+AAV+ DR SLVYG VR+TW +TLQRCT+ ASALV  G
Sbjct: 1   MEGMERCSANCVSLTPITFLERSAAVYGDRASLVYGRVRYTWRDTLQRCTRLASALVRKG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVA LAPN+PA YELHFAVPMAGA+LCTLNMRHDAAM+STLL HSEAKII VD+Q
Sbjct: 61  ISRGDVVAALAPNIPAMYELHFAVPMAGAVLCTLNMRHDAAMVSTLLSHSEAKIIFVDYQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L +VK AI++MSK T  E+LP +V++QE+D PSS ING DSA +DLE+ESLL TG +DF
Sbjct: 121 FLPMVKRAIKLMSKTT--EKLPLVVVIQEYDQPSSCINGVDSALEDLEFESLLATGEVDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +IRRP DEWDPISLNYTSGTTSRPKGVIYSHRG YLNTLST+LLNDM S+PVYLWTVPMF
Sbjct: 181 KIRRPKDEWDPISLNYTSGTTSRPKGVIYSHRGAYLNTLSTVLLNDMPSLPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW  +WGVAAQGGTNICQR V AKEIFANIS H VTHMGGAPTVLNMI+NAPI+EQK
Sbjct: 241 HCNGWCFSWGVAAQGGTNICQRTVTAKEIFANISQHNVTHMGGAPTVLNMIINAPISEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPS++LYKMR LGFLI+H YGLTET G AT C WKPEWDSLP+D+Q
Sbjct: 301 PLPGKVTVMTGGAPPPSHVLYKMRALGFLIIHSYGLTETYGPATVCSWKPEWDSLPEDQQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKL SRQG+QH+G+EG+DIK+P+TMES PADGKTMGEVM RGNTVMSGYLKDLKAT+EAF
Sbjct: 361 AKLNSRQGLQHVGLEGVDIKDPVTMESAPADGKTMGEVMFRGNTVMSGYLKDLKATQEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVL++HPS+LE AIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLYSHPSILEVAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFV LKDG +A  EEII+FCR+ LPHYMAP++VVF+ DLPK+STGKIQK +
Sbjct: 481 DDHWGETPCAFVKLKDGCSANGEEIIKFCREHLPHYMAPRSVVFR-DLPKTSTGKIQKFV 540

Query: 541 LKEEAKAMGSLSPAK 556
           LKEEA AMGSL  +K
Sbjct: 541 LKEEATAMGSLQRSK 552

BLAST of IVF0004705 vs. NCBI nr
Match: TYK19958.1 (putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 558/558 (100.00%), Postives = 558/558 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPAKRFD 558
           LKEEAKAMGSLSPAKRFD
Sbjct: 541 LKEEAKAMGSLSPAKRFD 558

BLAST of IVF0004705 vs. NCBI nr
Match: XP_008452948.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo])

HSP 1 Score: 1132 bits (2927), Expect = 0.0
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPAKR 556
           LKEEAKAMGSLSPAKR
Sbjct: 541 LKEEAKAMGSLSPAKR 556

BLAST of IVF0004705 vs. NCBI nr
Match: KAA0064634.1 (putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa])

HSP 1 Score: 1113 bits (2880), Expect = 0.0
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG
Sbjct: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ
Sbjct: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAK 546
           LKEEAK
Sbjct: 541 LKEEAK 546

BLAST of IVF0004705 vs. NCBI nr
Match: XP_004145598.1 (probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN55494.1 hypothetical protein Csa_012849 [Cucumis sativus])

HSP 1 Score: 1063 bits (2750), Expect = 0.0
Identity = 526/554 (94.95%), Postives = 536/554 (96.75%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEGI  CSANS+PLTPITFLERAAAVF DRISLVYGNVRFTW ETLQRCTKFASALVH+G
Sbjct: 1   MEGI-RCSANSVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLQRCTKFASALVHVG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAM+STLLGHSEAKIIV DHQ
Sbjct: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMVSTLLGHSEAKIIVADHQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           YLHIVKAAIEIMSK   AEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF
Sbjct: 121 YLHIVKAAIEIMSKTMAAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           EIRRPIDE DPISLNYTSGTTSRPKGVIYSHRGTYLNTLST+LLNDMSSM VYLWTVPMF
Sbjct: 181 EIRRPIDERDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTVLLNDMSSMAVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLH VTHMGGAPTVLNMIVNAPITEQK
Sbjct: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHNVTHMGGAPTVLNMIVNAPITEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PL GKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTET GVATFC WKPEWDSLPD+KQ
Sbjct: 301 PLLGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETYGVATFCHWKPEWDSLPDEKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIGVEG+DIKNPITMESVPADGKTMGEVMIRGNTVM GYLKD+KATEEAF
Sbjct: 361 AKLKSRQGMQHIGVEGVDIKNPITMESVPADGKTMGEVMIRGNTVMIGYLKDVKATEEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLK GSNAT+EEII+FCRD LPHYMAPKTVVFKSDLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKHGSNATEEEIIKFCRDNLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLSPA 554
           LKEEAKAMG+LS A
Sbjct: 541 LKEEAKAMGTLSLA 553

BLAST of IVF0004705 vs. NCBI nr
Match: XP_038899534.1 (probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida])

HSP 1 Score: 998 bits (2580), Expect = 0.0
Identity = 488/552 (88.41%), Postives = 517/552 (93.66%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEG   CSAN +PLTPITFLERAAAVF DRISLVYGNVRFTW ETL+RCTK ASALV LG
Sbjct: 1   MEGTDRCSANCVPLTPITFLERAAAVFGDRISLVYGNVRFTWRETLERCTKLASALVQLG 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISR DVVAVLAPNVPATYELHFAVPMA A+LCTLNMRHDA M+STLL HSEAKII+VD+Q
Sbjct: 61  ISRRDVVAVLAPNVPATYELHFAVPMASAVLCTLNMRHDATMVSTLLSHSEAKIIIVDYQ 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L IVKAAI+IMSK T  +ELPRIVIV+EFDHPSSNINGF+SAS+DLEYESLL+TG LDF
Sbjct: 121 FLSIVKAAIKIMSKTT-TKELPRIVIVKEFDHPSSNINGFESASNDLEYESLLDTGKLDF 180

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           +I+ P DEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF
Sbjct: 181 KIKHPKDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGWS+TWGVAAQGGTNICQRNV AKEIF NISLHKVTHMGGAPTVLNMI+NAPI EQK
Sbjct: 241 HCNGWSLTWGVAAQGGTNICQRNVTAKEIFTNISLHKVTHMGGAPTVLNMIINAPINEQK 300

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
            LP KVTVMTGGAPPPS++LYKMRE GFLIVHCYGLTETCG AT C WKPEWD+LPDDKQ
Sbjct: 301 SLPCKVTVMTGGAPPPSHVLYKMREFGFLIVHCYGLTETCGTATVCHWKPEWDALPDDKQ 360

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AKLKSRQGMQHIG+EG++IK+PITMESVPADGKTMGEVM RGNTVMSGY KD KAT+EAF
Sbjct: 361 AKLKSRQGMQHIGLEGVEIKDPITMESVPADGKTMGEVMFRGNTVMSGYFKDFKATQEAF 420

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
           NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENIS+IEVESVLFNHPSVLEAAIVGRP
Sbjct: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISSIEVESVLFNHPSVLEAAIVGRP 480

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           DDHWGETPCAFVNLKDG NAT+EEII+FCRD LPHYMAPKTVVFK DLPKSSTGKIQKLI
Sbjct: 481 DDHWGETPCAFVNLKDGFNATEEEIIKFCRDNLPHYMAPKTVVFK-DLPKSSTGKIQKLI 540

Query: 541 LKEEAKAMGSLS 552
           LKEEAKAM SL+
Sbjct: 541 LKEEAKAMDSLT 550

BLAST of IVF0004705 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 754.6 bits (1947), Expect = 5.7e-218
Identity = 353/556 (63.49%), Postives = 448/556 (80.58%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           MEG I   AN +PLTPI+FL+R+A V+ADR+S+VYG+V++TW +T  RC + ASAL  LG
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           IS GDVV+VLAPNVPA  ELHF VPMAGA+LCTLN+RHD+++++ LL HS  K+I  DHQ
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDF 180
           +L I + A EI+S     +++P +V++ E    + +++    + + +EYE ++  G  DF
Sbjct: 123 FLQIAEGACEILSNK--GDKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDF 182

Query: 181 EIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMF 240
           E+ RP DE D IS+NYTSGTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMF
Sbjct: 183 EVIRPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMF 242

Query: 241 HCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQK 300
           HCNGW + WGV A GGTNIC RNV AK IF NIS HKVTHMGGAPT+LNMI+NAP +EQK
Sbjct: 243 HCNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQK 302

Query: 301 PLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQ 360
           PLPGKV+ +TG APPP+++++KM ELGF + H YGLTET G  T C WKPEWDSLP ++Q
Sbjct: 303 PLPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQ 362

Query: 361 AKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAF 420
           AK+K+RQG+ H+G+E + +K+P+TM ++PADG TMGEV+ RGNTVM+GYLK+ +AT+EAF
Sbjct: 363 AKMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAF 422

Query: 421 NGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRP 480
            GGWF SGDLGV+HPDGYIELKDRSKDIIISGGENIS+IEVES LF HP VLEAA+V RP
Sbjct: 423 KGGWFWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 DDHWGETPCAFVNLKDGSNATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLI 540
           D++WGET CAFV LKDGS A+ EE+I +CRD+LPHYMAP+++VF+ DLPK+STGK+QK +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFE-DLPKTSTGKVQKFV 542

Query: 541 LKEEAKAMGSLSPAKR 557
           L+ +AKA+ SLS   R
Sbjct: 543 LRTKAKALVSLSKKGR 553

BLAST of IVF0004705 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 733.0 bits (1891), Expect = 1.8e-211
Identity = 353/557 (63.38%), Postives = 436/557 (78.28%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           +EG++   AN  PL+PITFLER+A V+ DR SLV+G+V+ TW +T QRC + ASAL +LG
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLG 107

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           ISRGDVVA LAPNVPA +ELHFAVPMAG ILC LN R D + +S LL HSEAKI+ VDHQ
Sbjct: 108 ISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQ 167

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEF----DHPSSNINGFDSA-SDDLEYESLLNT 180
            L I   A+++++K     +  ++V++ +     D    + + F S  S D EYE+LL +
Sbjct: 168 LLEIAHGALDLLAKSDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKS 227

Query: 181 GTLDFEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLW 240
           G  +FEI +P  EWDPIS+NYTSGTTSRPKGV+YSHRG YLN+L+T+ L+ MS  PVYLW
Sbjct: 228 GDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLW 287

Query: 241 TVPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAP 300
           TVPMFHCNGW + WGVAAQGGTNIC R V+ K IF NI++HKVTHMGGAPTVLNMIVN  
Sbjct: 288 TVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYT 347

Query: 301 ITEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSL 360
           +TE KPLP +V +MTGG+PP   IL KM ELGF + H YGLTET G  T C WKPEWDSL
Sbjct: 348 VTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSL 407

Query: 361 PDDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKA 420
             +++ KLK+RQG+QH+G+EGLD+K+P+TME+VP DG TMGEVM RGNTVMSGY KD++A
Sbjct: 408 SLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEA 467

Query: 421 TEEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAA 480
           T +AF G WF SGDL V++PDGYIE+KDR KD+IISGGENIS++EVE VL +H +VLEAA
Sbjct: 468 TRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAA 527

Query: 481 IVGRPDDHWGETPCAFVNLKDGSNATQ-EEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTG 540
           +V RPD HWG+TPC FV LK+G +  + EEII FCRD LPHYMAPKT+VF  D+PK+STG
Sbjct: 528 VVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVF-GDIPKTSTG 587

Query: 541 KIQKLILKEEAKAMGSL 552
           K+QK +L+++A  MGSL
Sbjct: 588 KVQKYLLRKKADEMGSL 603

BLAST of IVF0004705 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 653.7 bits (1685), Expect = 1.4e-187
Identity = 304/478 (63.60%), Postives = 386/478 (80.75%), Query Frame = 0

Query: 79  ELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQYLHIVKAAIEIMSKMTGA 138
           ELHF VPMAGA+LCTLN+RHD+++++ LL HS  K+I  DHQ+L I + A EI+S     
Sbjct: 3   ELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNK--G 62

Query: 139 EELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTGTLDFEIRRPIDEWDPISLNYTS 198
           +++P +V++ E    + +++    + + +EYE ++  G  DFE+ RP DE D IS+NYTS
Sbjct: 63  DKVPILVLIPE--PLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTS 122

Query: 199 GTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWTVPMFHCNGWSMTWGVAAQGGTN 258
           GTTS PKGV+YSHRG YLN+L+ +LLN+M S P YLWT PMFHCNGW + WGV A GGTN
Sbjct: 123 GTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTN 182

Query: 259 ICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPITEQKPLPGKVTVMTGGAPPPSN 318
           IC RNV AK IF NIS HKVTHMGGAPT+LNMI+NAP +EQKPLPGKV+ +TG APPP++
Sbjct: 183 ICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAH 242

Query: 319 ILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLPDDKQAKLKSRQGMQHIGVEGLD 378
           +++KM ELGF + H YGLTET G  T C WKPEWDSLP ++QAK+K+RQG+ H+G+E + 
Sbjct: 243 VIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQ 302

Query: 379 IKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKATEEAFNGGWFRSGDLGVRHPDGY 438
           +K+P+TM ++PADG TMGEV+ RGNTVM+GYLK+ +AT+EAF GGWF SGDLGV+HPDGY
Sbjct: 303 VKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGY 362

Query: 439 IELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAIVGRPDDHWGETPCAFVNLKDGS 498
           IELKDRSKDIIISGGENIS+IEVES LF HP VLEAA+V RPD++WGET CAFV LKDGS
Sbjct: 363 IELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGS 422

Query: 499 NATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSSTGKIQKLILKEEAKAMGSLSPAKR 557
            A+ EE+I +CRD+LPHYMAP+++VF+ DLPK+STGK+QK +L+ +AKA+ SLS   R
Sbjct: 423 KASAEELISYCRDRLPHYMAPRSIVFE-DLPKTSTGKVQKFVLRTKAKALVSLSKKGR 475

BLAST of IVF0004705 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 587.4 bits (1513), Expect = 1.2e-167
Identity = 277/555 (49.91%), Postives = 394/555 (70.99%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           M+ ++ C AN++PLTPITFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ L 
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           I+R DVV++LAPNVPA YE+HF+VPM GA+L  +N R DA  I+ +L H+E KI+ VD++
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG---- 180
           +  +++  + ++     ++  PRI+++ E D  +         S +L+YE L+  G    
Sbjct: 121 FAPLIQEVLRLIPTYQ-SQPHPRIILINEIDSTTKPF------SKELDYEGLIRKGEPTP 180

Query: 181 TLDFEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWT 240
           +    + R  +E DPISLNYTSGTT+ PKGV+ SH+G YL+ LS+I+  +M   PVYLWT
Sbjct: 181 SSSASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWT 240

Query: 241 VPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPI 300
           +PMFHCNGW+ TW VAA+GGTN+C R+V A EI+ NI LH VTHM   PTV   ++    
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300

Query: 301 TEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLP 360
           T+Q P    V V+TGG+ PP+ ++ K+ +LGF ++H YGLTE  G   FC+W+ EW+ LP
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360

Query: 361 DDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKAT 420
           + +Q +L+ RQG++++ +  +D+KN  T+ESVP DGKTMGE++I+G+++M GYLK+ KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420

Query: 421 EEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAI 480
            EAF  GW  +GD+GV HPDGY+E+KDRSKDIIISGGENIS+IEVE VL+ +  VLEAA+
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAV 480

Query: 481 VGRPDDHWGETPCAFVNLKDGSN---ATQEEIIEFCRDKLPHYMAPKTVVFKSDLPKSST 540
           V  P   WGETPCAFV LK G      ++ ++I++CR+ +PH+M PK VVF  +LPK+S 
Sbjct: 481 VAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSN 540

Query: 541 GKIQKLILKEEAKAM 549
           GKI K  L++ AKA+
Sbjct: 541 GKILKSKLRDIAKAL 548

BLAST of IVF0004705 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 579.7 bits (1493), Expect = 2.5e-165
Identity = 272/563 (48.31%), Postives = 390/563 (69.27%), Query Frame = 0

Query: 1   MEGIIPCSANSLPLTPITFLERAAAVFADRISLVYGNVRFTWGETLQRCTKFASALVHLG 60
           M+ +  C AN++PLTP+TFL+RA+  + +R S++YG  RFTW +T  RC + A++L+ L 
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  ISRGDVVAVLAPNVPATYELHFAVPMAGAILCTLNMRHDAAMISTLLGHSEAKIIVVDHQ 120
           IS+ DVV+V+APN PA YE+HFAVPMAGA+L  +N R DA  I+ +L H++ KI+ +D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YLHIVKAAIEIMSKMTGAEELPRIVIVQEFDHPSSNINGFDSASDDLEYESLLNTG---- 180
           +  + + ++ ++S       LP ++ + E D P        ++ ++L+YE L+  G    
Sbjct: 121 FEALARESLHLLSSEDSNLNLP-VIFIHENDFPKR------ASFEELDYECLIQRGEPTP 180

Query: 181 TLDFEIRRPIDEWDPISLNYTSGTTSRPKGVIYSHRGTYLNTLSTILLNDMSSMPVYLWT 240
           ++   + R  DE DPISLNYTSGTT+ PKGV+ SHRG YL TLS I+  +M + PVYLWT
Sbjct: 181 SMVARMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWT 240

Query: 241 VPMFHCNGWSMTWGVAAQGGTNICQRNVNAKEIFANISLHKVTHMGGAPTVLNMIVNAPI 300
           +PMFHCNGW+ TWG AA+GGT++C R+V A EI+ NI +H VTHM   PTV N+++    
Sbjct: 241 LPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNS 300

Query: 301 TEQKPLPGKVTVMTGGAPPPSNILYKMRELGFLIVHCYGLTETCGVATFCQWKPEWDSLP 360
            +  P  G V V+TGG+PPP+ ++ K++ LGF ++H YG TE  G   FC+W+ EW+ LP
Sbjct: 301 LDLSPRSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLP 360

Query: 361 DDKQAKLKSRQGMQHIGVEGLDIKNPITMESVPADGKTMGEVMIRGNTVMSGYLKDLKAT 420
           +++Q +LK+RQG+  +G+  +D+KN  T +S P DGKTMGE++I+G+++M GYLK+ KAT
Sbjct: 361 ENQQMELKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKAT 420

Query: 421 EEAFNGGWFRSGDLGVRHPDGYIELKDRSKDIIISGGENISTIEVESVLFNHPSVLEAAI 480
            EAF  GW  +GD+GV HPDG++E+KDRSKDIIISGGENIS++EVE+VL+ +P VLE A+
Sbjct: 421 FEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAV 480

Query: 481 VGRPDDHWGETPCAFVNLKDGSNATQEE-----------IIEFCRDKLPHYMAPKTVVFK 540
           V  P   WGETPCAFV L+      +E+           +IE+CR+ LPH+M P+ VVF 
Sbjct: 481 VAMPHPTWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFL 540

Query: 541 SDLPKSSTGKIQKLILKEEAKAM 549
            +LPK+  GKI K  L++ AK +
Sbjct: 541 EELPKNGNGKILKPKLRDIAKGL 556

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4HUK68.0e-21763.49Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1[more]
Q9SEY52.5e-21063.38Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1[more]
M4IRL41.2e-20462.59Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1[more]
M4IS929.5e-20261.87Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1[more]
I3PB361.2e-16950.00Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5D3D8V20.0e+00100.00Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A1S3BV310.0e+00100.00probable acyl-activating enzyme 1, peroxisomal OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5A7VBM00.0e+00100.00Putative acyl-activating enzyme 1, peroxisomal OS=Cucumis melo var. makuwa OX=11... [more]
A0A0A0L3B42.1e-30894.95Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G658540 PE=4 SV=1[more]
A0A6J1C8343.0e-26780.18probable acyl-activating enzyme 1, peroxisomal OS=Momordica charantia OX=3673 GN... [more]
Match NameE-valueIdentityDescription
TYK19958.10.0100.00putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa][more]
XP_008452948.10.0100.00PREDICTED: probable acyl-activating enzyme 1, peroxisomal [Cucumis melo][more]
KAA0064634.10.0100.00putative acyl-activating enzyme 1, peroxisomal [Cucumis melo var. makuwa][more]
XP_004145598.10.094.95probable acyl-activating enzyme 1, peroxisomal [Cucumis sativus] >KGN55494.1 hyp... [more]
XP_038899534.10.088.41probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G20560.15.7e-21863.49acyl activating enzyme 1 [more]
AT2G17650.11.8e-21163.38AMP-dependent synthetase and ligase family protein [more]
AT1G20560.21.4e-18763.60acyl activating enzyme 1 [more]
AT1G66120.11.2e-16749.91AMP-dependent synthetase and ligase family protein [more]
AT1G65880.12.5e-16548.31benzoyloxyglucosinolate 1 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 460..535
e-value: 6.3E-23
score: 81.6
NoneNo IPR availableGENE3D3.30.300.30coord: 447..549
e-value: 4.1E-31
score: 109.7
NoneNo IPR availablePANTHERPTHR43859:SF5ACYL-ACTIVATING ENZYME 2-RELATEDcoord: 5..551
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 5..551
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 13..543
e-value: 0.0
score: 845.812
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 10..547
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 20..451
e-value: 6.4E-94
score: 314.9
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 4..446
e-value: 1.0E-98
score: 332.7
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 194..205

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0004705.2IVF0004705.2mRNA