IVF0004488 (gene) Melon (IVF77) v1

Overview


Name	IVF0004488
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	DNA topoisomerase
Location	chr04: 2407929 .. 2412545 (-)
RNA-Seq Expression	IVF0004488
Synteny	IVF0004488

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCAGCTTCAGAGGAGCCTGTTATTCGCTTCTTCTTCCACTAACGCAATTTCTTCCTCTGTATTTTCTACTGCTCCCTCTGCTTCTTCCTGCTCCCGACTCCGTCCTTTCACGACCAAGGTTCTCATATTCTACCTATTATGAGTTCTATCCAGTTTTTCTTTTGTTCTTTTCAATAAATATTTTAACTGTTGCGTTTGGAGACTTACAATTTCTGTAGCGTTTGGTATTCGACTTTTTGGTTACTTCCGCTTATTTGTTCGTTTTTCTAGTTGTTTTAGGGTGAATTTATATATTTTTCGCATTGGAAGTGTGTTTGTCTACCGATTTTTATTCGCTGTTTAGCATGTTGTTTTTTTGAGTTATTTAAGTTATAGTCTTACATTCTGATTTTGGAGGTTTGTCTTGCAGCATGTATTACTTTTAGAAACTTAAGTGATAAGGGTCGATACAATCTCCTCAGAGAAAAGTTAGAAGAGTTGCAATTTTCTTATTGGCTTGTGTTTGGTATTATTTAAGTTACACTTACATTCTGATTCCGAAGTATAAACTTCTGGTGTTTGACTTTTTGGTTACTTCCGCTCACTTGTAGTTTTTCTGAATGAGTTCGAGCGGCTTAGTGAATTTACGTTTATATGGCAGTGTGATAATTTGTTTTCTATCAGTCTCTCTTTGCAGTTCCGTTGGTTGTGTTTGGGTTTTTTAATTCTCAGACTAATACACTCCTGTTTATGGATTCGTTTCGGACTTTTGCAGCAAATGTTAGAAATGGAATTTATGAACATTTTAAAAACTTATGTGGTAATGGGCAATCATATCTCATAAGGAAAACCATTCAAAAAGTTGCAGTTTGAATGTCTGTCAATGCTTTCTGCCATTTGACAATTTTAGTTCCTCTTGTTAACTTTCTCTTTTTCCCAAGTATTGTGCAGTCGCCTGGTGGTGATATTGTGTTATTTTGTTTGCCATTTGTGTTTCAGTTGCAGGGCAGCAATCTTCAAAATTATACTAGCAAATGCATACCTGTTTTACCATTTCCCACTGCTTGTAGTTCAGCCAGATGTTCTCAGCTTGTTTTTAGAAAAGGTGGAGTAACATTTGCTGTCAAGACTGTACGTGCAAAGGCTGGGCCAAGTGTTTTCACTCTGTATTCCCGCTTACCATTGAGATTTCCTCATTCTGCTGCATCACCAAATGCTTCTAACCAACAGCTGTTAAATGGCACTTTTACACATAAACCTCCTAATTATAGAGCGTTTGGAAGGAAGTTCTTTTCTAACACAACAGAAGCAAATTCAAGCAATCAGTTGGTTGGAGCTACAAAGGAGGCAAATCTAGTTTCATCTAGTTCTTCTTCATCCATTAGGAAAACATCCAAGGGCTTGAATGAAAAGAAGTCTTCGAGCAAGAAAAAGAAAGAAGCTATTTCTTCAGCCAATGGCAATGTTGAAGCCGTATGCAAGGATGGTCAGTCAATGAGCTTTGATAGTAGCAGTACTGACCTGGATGTTGTACAAGATCCAAAGGTTTGCAATTGGTTGATGTTTTCAATCATTTTTCTCATCTCATTGCATTATTTTAACTAACAGTGCAGTTTTTCAGAAAAATGTAACTGAGAGCAGTTCCAAAGATTCTGCTTCAACCCAACAACAACCCAATAAGAAAAAACGTAACTCCAGCAGAAAAAAAGAAGTTTCAAAAGATACTAATAAGTTGCCACAGGAGGCAAACGTCGTATTTGATGCAAAGTGGACATCTCAGGGAAGGACCAGTTTCAAGCAGCTATATCCCCCAATGGGAAAATCTGTTATGGTGGTGGAATCTGTTACAAAGGCAAAAGTTATTCAGAATTATCTTGGTGATATGTTTGTGGTGCTACCTAGCCATGGCCATGTTAGAGACTTGGCTGCGAAGTCAGGTTCTGTAAGACCTGATGATGACTTCAGTATGGTGTGGGAGGTTCCTTCTGCTGCTTATACTCATCTCAAAAACATGAAAGTTTCTTTAAATGGGTATGCATCTGGCTTCTATTGTTATTTTGGCCTGCATACCACTGTATTTTTGGCTAGTTTGATAATGATTGTTAAATTCATGTGTACACACACATGCACATTTGTATTTCTTTTATTAAGTTTGGCTTGCTTTTGTACACGGTTTGCATTGTTGTGCTTGTGCATTTGTGTCAATTGTTTTTGTGATGTCTAATATGCCTTACTTTCTATTTCTTCCAATTATGTCATTTTTTTTGTGATGTCTAATATACTTTCTTTTATCTTACCTCCAATAGCTTCAGTTTTAACTTTCTTTTCTTTCGCGGTGTGTTGATTCCTCAGAGCAGAAAACCTTATTCTTGCATCTGATCCAGATCGAGAAGGTGAAGCTATTGCGTGGCATATAATTGAGATGTTGCAGCACGAGGATGCTCTACATGAAGGTATTAGCATAGCGAGGGTTGTTTTTCATGAAATAACAGAGGCTTCTATAAAGAGTGCCCTGCAATCTCCTAGAGTAATTGATGCAAACTTGGTCCAGGCTTACCTTGCACGGCGTGCTTTAGATTATTTGATTGGGTTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCGATCAGCTGGGCGTGTTCAATCAGCTGCACTCGCCCTAATATGTGACAGAGAAATGGAAATTGATGAATTTCATGCACAAGAATATTGGACTATTGACATTAAGTTGAACCAAAAAGATACTTGTTCTTCAGTGGAAGATTTTGCAGTCCCTGCGCATTTGACCCATTTTGATTCTAAGAAGTTAAATCAATTTGCAATTAGCTCCAACATGGAGGCAAAGAATATAGAATCTGTGCTGAAATCAGTAAATTTTCAAGTGCTTAGCTCTAAAAAAAATAAAGTGCGGAGAAACCCTCCAATGCCATATATAACATCTACACTTCAGCAAGATGCAGCAAGTAAATTTAATTTCTCAACAACTTACACAATGCAGGTTGGTGGTAATTGTGTTTGCCCTCATCATGAGTTCTCTATGGTATAAAATAATGCAGTCGCGTTTAGTTAAAATTTTACTTCAAATCAGCATTCAGCACCAACTACTACTGTTTTACTGGATGCTGGTGTCTGTGCTAGTTGTAAGAGCCTGTTGGACATACCTCACCAAACTAATGATGATTTTATGTCGTGAGTTATCATCATCATTGTTTTTTATAATGAAACAATTACTTTTGTTTAGAAAAAAGATCATCTAAAAATCAAGTTCACTAAAGAGGGGAACACTGCTAAATAGGGGGCTGTAGCCGTACAAAATGGTGCTTATAGAATAGTTACAAAAGGTTTTCAAATTTGAAGGTTGGTGGAAAACATTGAATGGTCTGTATTCAAGTGGGCAGTCCTGTTATTAGATTTTGGGAGTGTTTTCCTCTGCATCTCAAACTCTGTTCTGTTTGTAGCGTTGTTTGTAAAAAATACTACCCTGTGAAAGTCAAATTGTTTTGCTGGTATATTGTGTTAGGGGTTCCCGATAAGGATAGTGGATCCAAATTATGAATCTTGTTTTTTGCCTGTCTTTAAGTTGGTGTCTTATATGATTTTCTATGCAGGTTGCCCAGAAACTTTATGAGGGAATTCAACTGGCTGACGGAAAGGCAGCTGGCCTAATAACTTATCTTAGAACAGATGGGCTACATGTAAAGACTTTTTGCTTTCAATTATATCTCCGTGCTGTCTCTTGTTATTTCTTTGGCAACACAGCTGGGTTTATCTTGTTGCCTAAGTCTATTTTTACCAATTAATTACTATGGGATTTTATTCTTTCTTTGATGTTACTGTTGTGATTTCTCGTATAAATGCATGGAAGAAAGTGAATTTAGTTTGTAGGTTGATGTTCTTTTTCACCTTTGTCCCAGTTGCTTTTTTGCCATTTCTAAAAAATCTTTCGTGCATCTAGTCTAATCCTAGTTGCTTGAACCACTTCCAATTTAGATCTCTGATGAAGCTGTTAAGGACATTCGCTCCCTGATTATGCAACGGTGAAAGTTCAAAATTCCACTTTTCTTTTGTTTGTAACTTATTTCAAATTTTTAGGTGCAATTTGTTTTCCCTCCTACCCATGTAAGGATTTTAGTGCATCTTATTATTTATTCTTAAAACGTACTGTTTACAGATATGGGAAGGATTTTGTACCTGAAAGTGGGCCCAAATATTTTAAGAAGGTGAAGAATGCTCAAGAGGCTCATGAAGCAATCAGACCCACTGATGTGCAACGATTACCCTGTAATTATATAATCTTCTCGCTGAATAATGCTTGATTTGGGAAAATATATATACATATATATATAAACATATAAGTTATTTTATTCTAGTAACATACTCCAACTTTGTTAACTTGAAGACTTTTTCTTTAGCCTTCCCATTTTTGTGAAAATATTTTTACCGATTATTGTTTGTTTTATTTCATTATTCACTTCCAGCAATGCTAGTGGGAATACTTGACGAAGATTCTCACAAACTATACTCTCTTATTTGGCTCAGAACGGTGGCATGTCAAATGGAATCTTCTATCACTGAACAGGTCAGGATGCATGTTCCATTATGCTATTTATTTTCTCTTATTTAG

mRNA sequence

ATGAGCCAGCTTCAGAGGAGCCTGTTATTCGCTTCTTCTTCCACTAACGCAATTTCTTCCTCTGTATTTTCTACTGCTCCCTCTGCTTCTTCCTGCTCCCGACTCCGTCCTTTCACGACCAAGTTGCAGGGCAGCAATCTTCAAAATTATACTAGCAAATGCATACCTGTTTTACCATTTCCCACTGCTTGTAGTTCAGCCAGATGTTCTCAGCTTGTTTTTAGAAAAGGTGGAGTAACATTTGCTGTCAAGACTGTACGTGCAAAGGCTGGGCCAAGTGTTTTCACTCTGTATTCCCGCTTACCATTGAGATTTCCTCATTCTGCTGCATCACCAAATGCTTCTAACCAACAGCTGTTAAATGGCACTTTTACACATAAACCTCCTAATTATAGAGCGTTTGGAAGGAAGTTCTTTTCTAACACAACAGAAGCAAATTCAAGCAATCAGTTGGTTGGAGCTACAAAGGAGGCAAATCTAGTTTCATCTAGTTCTTCTTCATCCATTAGGAAAACATCCAAGGGCTTGAATGAAAAGAAGTCTTCGAGCAAGAAAAAGAAAGAAGCTATTTCTTCAGCCAATGGCAATGTTGAAGCCGTATGCAAGGATGGTCAGTCAATGAGCTTTGATAGTAGCAGTACTGACCTGGATGTTGTACAAGATCCAAAGAAAAATGTAACTGAGAGCAGTTCCAAAGATTCTGCTTCAACCCAACAACAACCCAATAAGAAAAAACGTAACTCCAGCAGAAAAAAAGAAGTTTCAAAAGATACTAATAAGTTGCCACAGGAGGCAAACGTCGTATTTGATGCAAAGTGGACATCTCAGGGAAGGACCAGTTTCAAGCAGCTATATCCCCCAATGGGAAAATCTGTTATGGTGGTGGAATCTGTTACAAAGGCAAAAGTTATTCAGAATTATCTTGGTGATATGTTTGTGGTGCTACCTAGCCATGGCCATGTTAGAGACTTGGCTGCGAAGTCAGGTTCTGTAAGACCTGATGATGACTTCAGTATGGTGTGGGAGGTTCCTTCTGCTGCTTATACTCATCTCAAAAACATGAAAGTTTCTTTAAATGGAGCAGAAAACCTTATTCTTGCATCTGATCCAGATCGAGAAGGTGAAGCTATTGCGTGGCATATAATTGAGATGTTGCAGCACGAGGATGCTCTACATGAAGGTATTAGCATAGCGAGGGTTGTTTTTCATGAAATAACAGAGGCTTCTATAAAGAGTGCCCTGCAATCTCCTAGAGTAATTGATGCAAACTTGGTCCAGGCTTACCTTGCACGGCGTGCTTTAGATTATTTGATTGGGTTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCGATCAGCTGGGCGTGTTCAATCAGCTGCACTCGCCCTAATATGTGACAGAGAAATGGAAATTGATGAATTTCATGCACAAGAATATTGGACTATTGACATTAAGTTGAACCAAAAAGATACTTGTTCTTCAGTGGAAGATTTTGCAGTCCCTGCGCATTTGACCCATTTTGATTCTAAGAAGTTAAATCAATTTGCAATTAGCTCCAACATGGAGGCAAAGAATATAGAATCTGTGCTGAAATCAGTAAATTTTCAAGTGCTTAGCTCTAAAAAAAATAAAGTGCGGAGAAACCCTCCAATGCCATATATAACATCTACACTTCAGCAAGATGCAGCAAGTAAATTTAATTTCTCAACAACTTACACAATGCAGGTTGCCCAGAAACTTTATGAGGGAATTCAACTGGCTGACGGAAAGGCAGCTGGCCTAATAACTTATCTTAGAACAGATGGGCTACATATCTCTGATGAAGCTGTTAAGGACATTCGCTCCCTGATTATGCAACGATATGGGAAGGATTTTGTACCTGAAAGTGGGCCCAAATATTTTAAGAAGGTGAAGAATGCTCAAGAGGCTCATGAAGCAATCAGACCCACTGATGTGCAACGATTACCCTCAATGCTAGTGGGAATACTTGACGAAGATTCTCACAAACTATACTCTCTTATTTGGCTCAGAACGGTGGCATGTCAAATGGAATCTTCTATCACTGAACAGGTCAGGATGCATGTTCCATTATGCTATTTATTTTCTCTTATTTAG

Coding sequence (CDS)

ATGAGCCAGCTTCAGAGGAGCCTGTTATTCGCTTCTTCTTCCACTAACGCAATTTCTTCCTCTGTATTTTCTACTGCTCCCTCTGCTTCTTCCTGCTCCCGACTCCGTCCTTTCACGACCAAGTTGCAGGGCAGCAATCTTCAAAATTATACTAGCAAATGCATACCTGTTTTACCATTTCCCACTGCTTGTAGTTCAGCCAGATGTTCTCAGCTTGTTTTTAGAAAAGGTGGAGTAACATTTGCTGTCAAGACTGTACGTGCAAAGGCTGGGCCAAGTGTTTTCACTCTGTATTCCCGCTTACCATTGAGATTTCCTCATTCTGCTGCATCACCAAATGCTTCTAACCAACAGCTGTTAAATGGCACTTTTACACATAAACCTCCTAATTATAGAGCGTTTGGAAGGAAGTTCTTTTCTAACACAACAGAAGCAAATTCAAGCAATCAGTTGGTTGGAGCTACAAAGGAGGCAAATCTAGTTTCATCTAGTTCTTCTTCATCCATTAGGAAAACATCCAAGGGCTTGAATGAAAAGAAGTCTTCGAGCAAGAAAAAGAAAGAAGCTATTTCTTCAGCCAATGGCAATGTTGAAGCCGTATGCAAGGATGGTCAGTCAATGAGCTTTGATAGTAGCAGTACTGACCTGGATGTTGTACAAGATCCAAAGAAAAATGTAACTGAGAGCAGTTCCAAAGATTCTGCTTCAACCCAACAACAACCCAATAAGAAAAAACGTAACTCCAGCAGAAAAAAAGAAGTTTCAAAAGATACTAATAAGTTGCCACAGGAGGCAAACGTCGTATTTGATGCAAAGTGGACATCTCAGGGAAGGACCAGTTTCAAGCAGCTATATCCCCCAATGGGAAAATCTGTTATGGTGGTGGAATCTGTTACAAAGGCAAAAGTTATTCAGAATTATCTTGGTGATATGTTTGTGGTGCTACCTAGCCATGGCCATGTTAGAGACTTGGCTGCGAAGTCAGGTTCTGTAAGACCTGATGATGACTTCAGTATGGTGTGGGAGGTTCCTTCTGCTGCTTATACTCATCTCAAAAACATGAAAGTTTCTTTAAATGGAGCAGAAAACCTTATTCTTGCATCTGATCCAGATCGAGAAGGTGAAGCTATTGCGTGGCATATAATTGAGATGTTGCAGCACGAGGATGCTCTACATGAAGGTATTAGCATAGCGAGGGTTGTTTTTCATGAAATAACAGAGGCTTCTATAAAGAGTGCCCTGCAATCTCCTAGAGTAATTGATGCAAACTTGGTCCAGGCTTACCTTGCACGGCGTGCTTTAGATTATTTGATTGGGTTTAATATTTCACCATTATTATGGAGGAAATTACCAGGTTGCCGATCAGCTGGGCGTGTTCAATCAGCTGCACTCGCCCTAATATGTGACAGAGAAATGGAAATTGATGAATTTCATGCACAAGAATATTGGACTATTGACATTAAGTTGAACCAAAAAGATACTTGTTCTTCAGTGGAAGATTTTGCAGTCCCTGCGCATTTGACCCATTTTGATTCTAAGAAGTTAAATCAATTTGCAATTAGCTCCAACATGGAGGCAAAGAATATAGAATCTGTGCTGAAATCAGTAAATTTTCAAGTGCTTAGCTCTAAAAAAAATAAAGTGCGGAGAAACCCTCCAATGCCATATATAACATCTACACTTCAGCAAGATGCAGCAAGTAAATTTAATTTCTCAACAACTTACACAATGCAGGTTGCCCAGAAACTTTATGAGGGAATTCAACTGGCTGACGGAAAGGCAGCTGGCCTAATAACTTATCTTAGAACAGATGGGCTACATATCTCTGATGAAGCTGTTAAGGACATTCGCTCCCTGATTATGCAACGATATGGGAAGGATTTTGTACCTGAAAGTGGGCCCAAATATTTTAAGAAGGTGAAGAATGCTCAAGAGGCTCATGAAGCAATCAGACCCACTGATGTGCAACGATTACCCTCAATGCTAGTGGGAATACTTGACGAAGATTCTCACAAACTATACTCTCTTATTTGGCTCAGAACGGTGGCATGTCAAATGGAATCTTCTATCACTGAACAGGTCAGGATGCATGTTCCATTATGCTATTTATTTTCTCTTATTTAG

Protein sequence

MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPFPTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKKSSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQVRMHVPLCYLFSLI

Homology

[Close]

BLAST of IVF0004488 vs. ExPASy Swiss-Prot
Match: Q1RIM1 (DNA topoisomerase 1 OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=topA PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.0e-107
Identity = 203/403 (50.37%), Postives = 283/403 (70.22%), Query Frame = 0


Query: 292 VMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHL 351
           +++VES  KAK I  YLGD F V+ S GH+RDL +K GSV PD++FSM +++   A  ++
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVIPDENFSMKYDISEKAGKYV 62

Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
             +      AE++ LA+DPDREGE+I+WHI E+++ ++ +       RV F+EIT+ +I 
Sbjct: 63  DAIIKDAKKAESVYLATDPDREGESISWHIAEVIKEKNKVKSDDFFKRVAFNEITKKAIT 122

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDRE 471
            A+++PR +D NLV A  ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAIENPRKLDNNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 472 MEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIES 531
            EI+ F ++EYW I +K+      S+ E F   A LTH + +KL +F+I+++ EAK++  
Sbjct: 183 DEIERFKSEEYWDISLKMLN----SNNELFT--AKLTHINDQKLEKFSITNDKEAKDLTE 242

Query: 532 VLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLAD 591
            LKS NF V   +K + +R P  P+ITS+LQQ+AA K  FS   TMQ+AQKLYEG+ +  
Sbjct: 243 KLKSQNFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGK 302

Query: 592 GKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIR 651
            +  GLITY+RTDG+ +S++AV +IR LI + YG  ++P S   Y  KVKNAQEAHEAIR
Sbjct: 303 -ETIGLITYMRTDGVTLSNDAVDEIRKLINKDYGDKYLPSSPRIYKSKVKNAQEAHEAIR 362

Query: 652 PTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQV 695
           PT++  +P+ L   L++D +KLY LIW RT+ACQME+ I + V
Sbjct: 363 PTNINYIPNDLKEKLEKDYYKLYELIWKRTIACQMENVIMDLV 398

[Close]

BLAST of IVF0004488 vs. ExPASy Swiss-Prot
Match: Q4UM42 (DNA topoisomerase 1 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=topA PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 5.0e-107
Identity = 201/403 (49.88%), Postives = 279/403 (69.23%), Query Frame = 0


Query: 292 VMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHL 351
           +++VES  KAK I  YLGD F V+ S GH+RDL +K GSV PD++F+M +++   A  ++
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYV 62

Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
             +      A+ + LA+DPDREGEAI+WH+ E+++ ++ +       RV F+EIT+ +I 
Sbjct: 63  DAIVKDAKKADAVYLATDPDREGEAISWHVAEVIKEKNKVKSDDFFKRVAFNEITKKAII 122

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDRE 471
            A+++PR +DANLV A  ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAVENPRKLDANLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 472 MEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIES 531
            EI+ F ++EYW I +K+      S+ E F   A LTH + +KL +F+I +  +AK++  
Sbjct: 183 DEIERFKSEEYWDISLKMQN----SNNELFT--AKLTHVNDQKLEKFSIINEKDAKDLTE 242

Query: 532 VLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLAD 591
            LKS NF V   +K + +R P  P+ITS+LQQ+AA K  FS   TMQ+AQKLYEG+ +  
Sbjct: 243 KLKSQNFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGK 302

Query: 592 GKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIR 651
            +  GLITY+RTDG+ +S++A+ DIR LI + YG  ++P S   Y  KVKNAQEAHEAIR
Sbjct: 303 -ETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGDKYLPNSPRIYKSKVKNAQEAHEAIR 362

Query: 652 PTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQV 695
           PT++   P  L   L++D +KLY LIW RT+ACQME+ I + V
Sbjct: 363 PTNITYTPDSLKEKLEKDYYKLYELIWKRTIACQMENVIMDLV 398

[Close]

BLAST of IVF0004488 vs. ExPASy Swiss-Prot
Match: Q92IH1 (DNA topoisomerase 1 OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=topA PE=3 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 2.1e-105
Identity = 199/403 (49.38%), Postives = 276/403 (68.49%), Query Frame = 0


Query: 292 VMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHL 351
           +++VES  KAK I  YLGD F V+ S GH+RDL +K GSV PD++F+M +++   A  ++
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDENFAMKYDISDKAGKYV 62

Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
             +      A+ + LA+DPDREGE+I+WH+ E+++ ++ +       RV F+EIT+ +I 
Sbjct: 63  DAIVKDAKKADAVYLATDPDREGESISWHVAEVIKEKNQVKSDDFFKRVAFNEITKKAII 122

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDRE 471
            A+++PR +D NLV A  ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LIC+RE
Sbjct: 123 HAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICERE 182

Query: 472 MEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIES 531
            EI+ F ++EYW I +K+      S+ E F   A LTH + +KL +F+I +   AK++  
Sbjct: 183 DEIERFKSEEYWDISLKMQN----SNNELFT--AKLTHVNDQKLEKFSIINEKNAKDLTK 242

Query: 532 VLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLAD 591
            LKS  F V   +K + +R P  P+ITS+LQQ+AA K  FS   TMQ+AQKLYEG+ +  
Sbjct: 243 KLKSHKFHVDKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGK 302

Query: 592 GKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIR 651
            +  GLITY+RTDG+ +S++A+ DIR LI + YG  ++P S   Y  KVKNAQEAHEAIR
Sbjct: 303 -ETIGLITYMRTDGVTLSNDAIADIRKLIDKSYGDKYLPTSPRIYKSKVKNAQEAHEAIR 362

Query: 652 PTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQV 695
           PT++   P  L   LD+D +KLY LIW RT+ACQME+ I + V
Sbjct: 363 PTNITYTPDSLKEKLDKDYYKLYELIWKRTIACQMENVIMDLV 398

[Close]

BLAST of IVF0004488 vs. ExPASy Swiss-Prot
Match: Q68X45 (DNA topoisomerase 1 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257363 GN=topA PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.6e-105
Identity = 200/403 (49.63%), Postives = 275/403 (68.24%), Query Frame = 0


Query: 292 VMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHL 351
           +++VES  KAK I  YLGD F V+ S GH+RDL +K GSV PD +F+M +++   A  ++
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFAMEYDISDKASKYV 62

Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
             +      AE + LA+DPDREGE+I+WH+ E+++ ++ +       RV F+EIT+ +I 
Sbjct: 63  DAIVKYARKAEAVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAII 122

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDRE 471
            A+++PR +D NLV A  ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LICDRE
Sbjct: 123 HAVENPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDRE 182

Query: 472 MEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIES 531
            EI+ F A+EYW I +K+      S+ E F   A LTH + +KL +F+I +  EAK++  
Sbjct: 183 DEIERFKAEEYWDISLKMQN----SNNELFT--AKLTHVNDQKLKKFSIINEKEAKDLTR 242

Query: 532 VLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLAD 591
            LK   F V   +K + +R P  P+ITS+LQQ+AA K  FS   TMQ+AQKLYEG+ +  
Sbjct: 243 KLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGK 302

Query: 592 GKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIR 651
            +  GLITY+RTDG+ +S++A+ DIR LI + YG  ++P +   Y  KVKNAQEAHEAIR
Sbjct: 303 -ETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNKYLPINPRIYQSKVKNAQEAHEAIR 362

Query: 652 PTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQV 695
           PT++   P  L   L++D +KLY LIW RT+ACQME+ I + V
Sbjct: 363 PTNITYTPDNLKQKLEKDYYKLYELIWQRTIACQMENVIMDLV 398

[Close]

BLAST of IVF0004488 vs. ExPASy Swiss-Prot
Match: Q9ZDK2 (DNA topoisomerase 1 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=topA PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.8e-104
Identity = 196/403 (48.64%), Postives = 271/403 (67.25%), Query Frame = 0


Query: 292 VMVVESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHL 351
           +++VES  KAK I  YLGD F V+ S GH+RDL +K GSV PD +F M +++   A  ++
Sbjct: 3   LVIVESPAKAKTINKYLGDEFKVIASFGHIRDLPSKKGSVLPDKNFLMEYDISDKAGKYV 62

Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
             +      AE + LA+DPDREGE+I+WH+ E+++ ++ +       RV F+EIT+ +I 
Sbjct: 63  DAIVKEARKAEVVYLATDPDREGESISWHVAEVIKEKNKVESDDFFKRVAFNEITKKAIM 122

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDRE 471
           +A+ +PR +D NLV A  ARRALDYL+GF +SPLLWRKLPGC+SAGRVQS AL LICDRE
Sbjct: 123 NAVANPRKLDTNLVNAQQARRALDYLVGFTLSPLLWRKLPGCKSAGRVQSVALRLICDRE 182

Query: 472 MEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIES 531
            EI+ F ++EYW I +K+   +      +    A LTH + +KL +F+I +  EAK++  
Sbjct: 183 DEIERFKSEEYWDISLKMQNSN------NDLFTAKLTHVNDQKLKKFSIINEKEAKDLTQ 242

Query: 532 VLKSVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLAD 591
            LK   F V   +K + +R P  P+ITS+LQQ+AA K  FS   TMQ+AQKLYEG+ +  
Sbjct: 243 KLKLQKFYVEKIEKKQQKRQPQPPFITSSLQQEAARKLGFSAKKTMQIAQKLYEGVDIGK 302

Query: 592 GKAAGLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIR 651
            +  GLITY+RTDG+ +S++A+ DIR LI + YG  ++P     Y  KVKNAQEAHEAIR
Sbjct: 303 -ETIGLITYMRTDGVTLSNDAIADIRKLIDKNYGNQYLPIKPRIYQSKVKNAQEAHEAIR 362

Query: 652 PTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQMESSITEQV 695
           PT++   P  L   L++D +KLY LIW RT+ACQME+ I + V
Sbjct: 363 PTNITYTPDSLKQKLEKDYYKLYELIWHRTIACQMENVIMDLV 398

[Close]

BLAST of IVF0004488 vs. ExPASy TrEMBL
Match: A0A5A7TAX8 (Omega-protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006510 PE=3 SV=1)

HSP 1 Score: 1324.7 bits (3427), Expect = 0.0e+00
Identity = 693/696 (99.57%), Postives = 696/696 (100.00%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL 120
           PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL
Sbjct: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL 120

Query: 121 NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK 180
           NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK
Sbjct: 121 NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK 180

Query: 181 SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ 240
           SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ
Sbjct: 181 SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ 240

Query: 241 PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK 300
           PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK
Sbjct: 241 PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK 300

Query: 301 AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG 360
           AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG
Sbjct: 301 AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG 360

Query: 361 AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI 420
           AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI
Sbjct: 361 AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI 420

Query: 421 DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ 480
           DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ
Sbjct: 421 DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ 480

Query: 481 EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV 540
           EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV
Sbjct: 481 EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV 540

Query: 541 LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY 600
           LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY
Sbjct: 541 LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY 600

Query: 601 LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS 660
           LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS
Sbjct: 601 LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS 660

Query: 661 MLVGILDEDSHKLYSLIWLRTVACQMESSITEQVRM 697
           MLVGILDEDSHKLYSLIWLRTVACQMESSITEQ+++
Sbjct: 661 MLVGILDEDSHKLYSLIWLRTVACQMESSITEQIQI 696

[Close]

BLAST of IVF0004488 vs. ExPASy TrEMBL
Match: A0A5A7T7A4 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006520 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 600/822 (72.99%), Postives = 645/822 (78.47%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKA-------GPSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKA       GPSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 697
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. ExPASy TrEMBL
Match: A0A1S4E6F1 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103503742 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 600/822 (72.99%), Postives = 645/822 (78.47%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKA-------GPSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKA       GPSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 697
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. ExPASy TrEMBL
Match: A0A1S4E5M8 (Omega-protein OS=Cucumis melo OX=3656 GN=LOC103503742 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 600/822 (72.99%), Postives = 645/822 (78.47%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKA-------GPSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKA       GPSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 697
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. ExPASy TrEMBL
Match: A0A6J1IF86 (Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1)

HSP 1 Score: 1026.2 bits (2652), Expect = 6.6e-296
Identity = 569/761 (74.77%), Postives = 614/761 (80.68%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           M QLQRSL  A++S+NA+SSSVFST PSA S SRLRP   KLQ    QN TSKCIPVLPF
Sbjct: 1   MRQLQRSLFMATTSSNAVSSSVFSTTPSA-SFSRLRPLMAKLQSRTSQNNTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKA-------GPS-VFTLYSRLPLRFPHSAASP 120
            T C+ A+CSQLVFRK  VTFAVKT RAKA       GPS VFTL+SRLPL F HS ASP
Sbjct: 61  STVCNYAKCSQLVFRKSRVTFAVKTERAKAGQCRTCDGPSCVFTLHSRLPLGFHHSVASP 120

Query: 121 NASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA--------------------------- 180
           NAS +Q+LNGTFT   PNYRAF +KFFS +TEA                           
Sbjct: 121 NASKRQVLNGTFTPTFPNYRAFEKKFFSQSTEALNKTENENVKYASNGTSTFPFEGLNKK 180

Query: 181 -----------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGL 240
                                        NSS+QLVG TKEA LVSS+SSSS+RK SKGL
Sbjct: 181 WKGAKPVATSRKKQISASGSTGSTSVVGTNSSDQLVGVTKEAGLVSSNSSSSVRKASKGL 240

Query: 241 NEKKSSSKKKKEAISSAN-GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSA 300
            EK S SKKKKEAIS  N  +VEAVCKD QS S DSS+ D +VVQ+PKK  +  ++K S 
Sbjct: 241 KEKNSGSKKKKEAISPTNSSDVEAVCKDSQSKSLDSSNVDKNVVQNPKKKSSGGTAKGSV 300

Query: 301 STQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVV 360
           ST QQ NKKK NSSRKKE  KD +KLPQ+ N+V DA+  SQG T+ KQLYPPMGKSVMVV
Sbjct: 301 STTQQSNKKKGNSSRKKEAQKDASKLPQKPNLVVDARGKSQGITTLKQLYPPMGKSVMVV 360

Query: 361 ESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMK 420
           ESVTKAKVIQNYLGDMF VLPSHGHVRDLAA+SGSVRPDDDFSMVWEVPSAA+THLK+MK
Sbjct: 361 ESVTKAKVIQNYLGDMFEVLPSHGHVRDLAARSGSVRPDDDFSMVWEVPSAAWTHLKSMK 420

Query: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQ 480
           VSLNGAENLILASDPDREGEAIAWHIIEMLQ +DALHEGISIARVVFHEITEASIKSALQ
Sbjct: 421 VSLNGAENLILASDPDREGEAIAWHIIEMLQQQDALHEGISIARVVFHEITEASIKSALQ 480

Query: 481 SPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEID 540
           SPR IDANLVQAYLARRALDYLIGFNISPLLWRKLPGC+SAGRVQSAALALICDRE+EI+
Sbjct: 481 SPRGIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCQSAGRVQSAALALICDRELEIE 540

Query: 541 EFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKS 600
           EF  QEYWTIDI+LNQKD CSSVE+F VPAHLTHFDSKKLNQFAISS  EAKNIE+ LKS
Sbjct: 541 EFQPQEYWTIDIELNQKDPCSSVEEFTVPAHLTHFDSKKLNQFAISSTTEAKNIETALKS 600

Query: 601 VNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAA 660
           VNFQVLSSKKNKVRRNPPMPYITSTLQQDAA+K NF  +YTM++AQKLYEGIQLADGKAA
Sbjct: 601 VNFQVLSSKKNKVRRNPPMPYITSTLQQDAANKHNFPASYTMKLAQKLYEGIQLADGKAA 660

Query: 661 GLITYLRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDV 697
           GLITY RTDGLHISDEAVKDIRSLI QRYG +FV ES  KYFKKVKNAQEAHEAIRPTDV
Sbjct: 661 GLITYPRTDGLHISDEAVKDIRSLIKQRYGDNFVSESARKYFKKVKNAQEAHEAIRPTDV 720

[Close]

BLAST of IVF0004488 vs. NCBI nr
Match: KAA0038737.1 (DNA topoisomerase 1 isoform X2 [Cucumis melo var. makuwa] >TYK31350.1 DNA topoisomerase 1 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1320 bits (3416), Expect = 0.0
Identity = 693/697 (99.43%), Postives = 696/697 (99.86%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL 120
           PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL
Sbjct: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQLL 120

Query: 121 NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK 180
           NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK
Sbjct: 121 NGTFTHKPPNYRAFGRKFFSNTTEANSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKK 180

Query: 181 SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ 240
           SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ
Sbjct: 181 SSSKKKKEAISSANGNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQ 240

Query: 241 PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK 300
           PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK
Sbjct: 241 PNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTK 300

Query: 301 AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG 360
           AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG
Sbjct: 301 AKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNG 360

Query: 361 AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI 420
           AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI
Sbjct: 361 AENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVI 420

Query: 421 DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ 480
           DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ
Sbjct: 421 DANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQ 480

Query: 481 EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV 540
           EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV
Sbjct: 481 EYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQV 540

Query: 541 LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY 600
           LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY
Sbjct: 541 LSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITY 600

Query: 601 LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS 660
           LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS
Sbjct: 601 LRTDGLHISDEAVKDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPS 660

Query: 661 MLVGILDEDSHKLYSLIWLRTVACQMESSITEQVRMH 697
           MLVGILDEDSHKLYSLIWLRTVACQMESSITEQ+++ 
Sbjct: 661 MLVGILDEDSHKLYSLIWLRTVACQMESSITEQIQIE 697

[Close]

BLAST of IVF0004488 vs. NCBI nr
Match: XP_011652536.2 (uncharacterized protein LOC101210581 isoform X1 [Cucumis sativus])

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 625/744 (84.01%), Postives = 655/744 (88.04%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRS+L ASSS+NAISSSVFSTAPSASS SRLRPF  KLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSMLLASSSSNAISSSVFSTAPSASSSSRLRPFRIKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKT--VRAKAGPSVFTLYSRLPLRFPHSAASPNASNQQ 120
            T C+SARCSQL FRKGGVTFA+K    RA+ GPSVFTLYSRLPLRFPHSAASP  SNQQ
Sbjct: 61  STVCNSARCSQLGFRKGGVTFAIKAGQYRAREGPSVFTLYSRLPLRFPHSAASPIVSNQQ 120

Query: 121 LLNGTFTHKPPNYRAFGRKFFSNTTEA--------------------------------- 180
           LLN   THK P+YR  GRKFFSNTTEA                                 
Sbjct: 121 LLN--CTHKFPDYRESGRKFFSNTTEALNGTSAFPFEGLNKNWKVAKPVATSRKKRISAS 180

Query: 181 -----------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNEKKSSSKKKKEAISSAN 240
                      NSSNQLVGATKEANLVSSSSSSSIRK SKGLNEKKS SKKKKEAISS N
Sbjct: 181 EPLGSTSVDGTNSSNQLVGATKEANLVSSSSSSSIRKASKGLNEKKSWSKKKKEAISSTN 240

Query: 241 -GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDSASTQQQPNKKKRNSSRKKE 300
             +VEAV KDGQ+ SFD S+ D DVVQDPKKNVT  SSKDSASTQQQ NKKKRNSSRKKE
Sbjct: 241 DADVEAVGKDGQAKSFDRSNIDQDVVQDPKKNVTGGSSKDSASTQQQSNKKKRNSSRKKE 300

Query: 301 VSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTKAKVIQNYLGDMFV 360
           V KD NKLPQEA+VVFDA+WTSQGRT+FKQLYPPMGKSVMVVESVTKAKVIQNYLGDMFV
Sbjct: 301 VLKDANKLPQEASVVFDARWTSQGRTTFKQLYPPMGKSVMVVESVTKAKVIQNYLGDMFV 360

Query: 361 VLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNGAENLILASDPDRE 420
           VLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAA THLK+M+VSLNGAENLILASDPDRE
Sbjct: 361 VLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAALTHLKSMQVSLNGAENLILASDPDRE 420

Query: 421 GEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVIDANLVQAYLARRA 480
           GEAIAWHIIEML H+DALHEGISIARVVFHEITEASIKSALQSPRVIDANLVQAYLARRA
Sbjct: 421 GEAIAWHIIEMLHHQDALHEGISIARVVFHEITEASIKSALQSPRVIDANLVQAYLARRA 480

Query: 481 LDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQEYWTIDIKLNQKD 540
           LDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQEYW+IDI+LNQK+
Sbjct: 481 LDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQEYWSIDIELNQKE 540

Query: 541 TCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQVLSSKKNKVRRNPP 600
           +CS VEDFAVPAHLTHFDS+KLNQFAISSN EAKNIE+ LKSVNFQVLSSKK KVRRNPP
Sbjct: 541 SCSLVEDFAVPAHLTHFDSRKLNQFAISSNTEAKNIETALKSVNFQVLSSKKTKVRRNPP 600

Query: 601 MPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITYLRTDGLHISDEAV 660
           MPYITSTLQQDAA+KFNFS +YTMQ+AQKLYEGIQLADGKAAGLITYLRTDGLHISDEAV
Sbjct: 601 MPYITSTLQQDAANKFNFSASYTMQLAQKLYEGIQLADGKAAGLITYLRTDGLHISDEAV 660

Query: 661 KDIRSLIMQRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPSMLVGILDEDSHKL 697
           KDIR+LIMQRYG++FVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPSMLVG LDEDSHKL
Sbjct: 661 KDIRTLIMQRYGQNFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPSMLVGTLDEDSHKL 720

[Close]

BLAST of IVF0004488 vs. NCBI nr
Match: KAA0038738.1 (DNA topoisomerase 1 isoform X1 [Cucumis melo var. makuwa] >TYK31351.1 DNA topoisomerase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 601/826 (72.76%), Postives = 646/826 (78.21%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAG-------PSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKAG       PSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 700
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. NCBI nr
Match: XP_016903546.1 (PREDICTED: DNA topoisomerase 1 isoform X2 [Cucumis melo])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 601/826 (72.76%), Postives = 646/826 (78.21%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAG-------PSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKAG       PSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 700
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. NCBI nr
Match: XP_016903545.1 (PREDICTED: DNA topoisomerase 1 isoform X1 [Cucumis melo])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 601/826 (72.76%), Postives = 646/826 (78.21%), Query Frame = 0


Query: 1   MSQLQRSLLFASSSTNAISSSVFSTAPSASSCSRLRPFTTKLQGSNLQNYTSKCIPVLPF 60
           MSQLQRSL  ASSS+NAISSSVFSTA SA S SRLR F+TKLQGS LQNYTSKCIPVLPF
Sbjct: 1   MSQLQRSLSLASSSSNAISSSVFSTALSAFSSSRLRHFSTKLQGSTLQNYTSKCIPVLPF 60

Query: 61  PTACSSARCSQLVFRKGGVTFAVKTVRAKAG-------PSVFTLYSRLPLRFPHSAASPN 120
           PTACSSARCSQL+FRKGGVTFAVKT RAKAG       PSVFTLYSRLPLRFPHSAASPN
Sbjct: 61  PTACSSARCSQLIFRKGGVTFAVKTERAKAGQYRACDGPSVFTLYSRLPLRFPHSAASPN 120

Query: 121 ASNQQLLNGTFTHKPPNYRAFGRKFFSNTTEA---------------------------- 180
           ASNQQLLN TFTHK PNYRAFGRKFFSNTTEA                            
Sbjct: 121 ASNQQLLNCTFTHKLPNYRAFGRKFFSNTTEASSGTKNKNVKYLRNGTSTIEGLNKKSKG 180

Query: 181 ---------------------------NSSNQLVGATKEANLVSSSSSSSIRKTSKGLNE 240
                                      NSS+QLVGATKEANLVSS+SSSSIR+ SKGLNE
Sbjct: 181 AKPVATSRKKTRISASAPSGSTHVDGTNSSDQLVGATKEANLVSSNSSSSIRRASKGLNE 240

Query: 241 KKSSSKKKKEAISSAN-------------------------------------------- 300
           KKS SKKKK+AISS N                                            
Sbjct: 241 KKSWSKKKKKAISSTNDADVEAVCKDSQSKSFGSSNTDQDVVQDPKKEAISSTIRKASKG 300

Query: 301 --------------------GNVEAVCKDGQSMSFDSSSTDLDVVQDPKKNVTESSSKDS 360
                                +VEAVCKDGQS SF SS+TD DVVQDPKKNVT+ +SKDS
Sbjct: 301 LNEKKSWSKNKKEAISSTNDADVEAVCKDGQSKSFGSSNTDQDVVQDPKKNVTKGTSKDS 360

Query: 361 ASTQQQPNKKKRNSSRKKEVSKDTNKLPQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMV 420
            ST+QQ NKKK NSSR KE +K  NKL Q+ NV+ D + TSQG+T+FKQLYPPMGKSV+V
Sbjct: 361 GSTKQQSNKKKCNSSRNKEATKGANKLQQKPNVIVDTRGTSQGKTTFKQLYPPMGKSVLV 420

Query: 421 VESVTKAKVIQNYLGDMFVVLPSHGHVRDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNM 480
           VESVTKAKVIQ+YLGDMFVVLPSHGHVRDLAA+SGSVRP+DDF MVWEVPSAA+THLK++
Sbjct: 421 VESVTKAKVIQDYLGDMFVVLPSHGHVRDLAARSGSVRPEDDFIMVWEVPSAAWTHLKSI 480

Query: 481 KVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIKSAL 540
           ++SLNGAENLILASDPD+EGEAIAWHIIEMLQH+ ALHEGISIARVVFHEITEASIKSAL
Sbjct: 481 ELSLNGAENLILASDPDQEGEAIAWHIIEMLQHQHALHEGISIARVVFHEITEASIKSAL 540

Query: 541 QSPRVIDANLVQAYLARRALDYLIGFNISPLLWRKLPGCRSAGRVQSAALALICDREMEI 600
           QSPRVID NLVQAYLARRALDYLIGFNISPLLW+KLPGCRS GRVQSAALALICDRE EI
Sbjct: 541 QSPRVIDENLVQAYLARRALDYLIGFNISPLLWKKLPGCRSPGRVQSAALALICDRETEI 600

Query: 601 DEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQFAISSNMEAKNIESVLK 660
           D+F AQEYWTID+KLNQKD CSSVEDFAVPAHLTHFDSKKLNQF ISSNMEAKNIE+ LK
Sbjct: 601 DKFQAQEYWTIDVKLNQKDPCSSVEDFAVPAHLTHFDSKKLNQFPISSNMEAKNIETALK 660

Query: 661 SVNFQVLSSKKNKVRRNPPMPYITSTLQQDAASKFNFSTTYTMQVAQKLYEGIQLADGKA 700
           SVNFQVLSS+KN V+ NPPMPYITSTLQQDAA KFNF  TYTM++AQKLYEGIQLADGKA
Sbjct: 661 SVNFQVLSSRKNIVQTNPPMPYITSTLQQDAAKKFNFPATYTMKLAQKLYEGIQLADGKA 720

[Close]

BLAST of IVF0004488 vs. TAIR 10
Match: AT4G31210.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 585.9 bits (1509), Expect = 4.4e-167
Identity = 320/557 (57.45%), Postives = 411/557 (73.79%), Query Frame = 0


Query: 153 GATKEANLVSSSSSSSIRKTSKGLNEKKSSSKKKKEAISSANGNVEAVCKDGQSMSF--- 212
           G   E +  +S +S+  + T+   ++++  +KK ++  SS    +EAV  D   +S    
Sbjct: 336 GIKAELSTAASPASNGNQATTV-KSKRRPKNKKVEDKSSSVVPVLEAVSLDESPISVPKP 395

Query: 213 --------DSSSTDLDVVQDPKKNVTESSSKDSASTQQQPNKKKRNSSRKKEVSKDTNKL 272
                    SSS   +V ++      +S +  ++ ++QQ  K  + S   K+       +
Sbjct: 396 KHSGSGNRKSSSAKKEVAKNHPVEEPKSPAPSNSKSEQQHLKSTKASKAPKQ-----KLV 455

Query: 273 PQEANVVFDAKWTSQGRTSFKQLYPPMGKSVMVVESVTKAKVIQNYLGDMFVVLPSHGHV 332
           PQ      +     +G+ + K LYPP GKSV+VVES+TKAK+IQ YLGDM+ VLPS+GH+
Sbjct: 456 PQHMKNSIE----HRGQNASKPLYPPSGKSVIVVESMTKAKIIQGYLGDMYEVLPSYGHI 515

Query: 333 RDLAAKSGSVRPDDDFSMVWEVPSAAYTHLKNMKVSLNGAENLILASDPDREGEAIAWHI 392
           RDLA +SGSVRPDDDFSMVWEVPS+A+TH+K++KV+LNGAENLILASDPDREGEAIAWHI
Sbjct: 516 RDLATRSGSVRPDDDFSMVWEVPSSAWTHIKSIKVALNGAENLILASDPDREGEAIAWHI 575

Query: 393 IEMLQHEDALHEGISIARVVFHEITEASIKSALQSPRVIDANLVQAYLARRALDYLIGFN 452
           IEMLQ + ALHE +++ARVVFHEITE++IKSALQSPR ID +LV AYLARRALDYLIGFN
Sbjct: 576 IEMLQQQGALHESMTVARVVFHEITESAIKSALQSPREIDGDLVHAYLARRALDYLIGFN 635

Query: 453 ISPLLWRKLPGCRSAGRVQSAALALICDREMEIDEFHAQEYWTIDIKLNQKDTCSSVEDF 512
           ISPLLWRKLPGC SAGRVQSAALAL+CDRE EID F  QEYWT+ IK+  KD  ++    
Sbjct: 636 ISPLLWRKLPGCPSAGRVQSAALALVCDRESEIDGFKPQEYWTVGIKVKGKDNSATFS-- 695

Query: 513 AVPAHLTHFDSKKLNQFAISSNMEAKNIESVLKSVNFQVLSSKKNKVRRNPPMPYITSTL 572
              AHLT  +SK+LNQ +ISS   A++IE  +KS  F V  +K +  R+NPP PYITSTL
Sbjct: 696 ---AHLTSLNSKRLNQLSISSEANAQDIEQRIKSEGFLVKGTKTSTTRKNPPTPYITSTL 755

Query: 573 QQDAASKFNFSTTYTMQVAQKLYEGIQLADGKAAGLITYLRTDGLHISDEAVKDIRSLIM 632
           QQDAA+K +FST +TM++AQKLYEG+QL+DGK+AGLITY+RTDGLHI+DEA+KDI+SL+ 
Sbjct: 756 QQDAANKLHFSTAHTMKLAQKLYEGVQLSDGKSAGLITYMRTDGLHIADEAIKDIQSLVA 815

Query: 633 QRYGKDFVPESGPKYFKKVKNAQEAHEAIRPTDVQRLPSMLVGILDEDSHKLYSLIWLRT 692
           +RYGK+F  +S  KYFKKVKNAQEAHEAIRPTD++RLPS +  +LD DS KLY+LIW R 
Sbjct: 816 ERYGKNFTSDSPRKYFKKVKNAQEAHEAIRPTDIRRLPSTIASLLDADSLKLYTLIWSRA 875

Query: 693 VACQMESSITEQVRMHV 699
           VACQME +   Q+++ +
Sbjct: 876 VACQMEPASIAQIQLDI 877

[Close]

BLAST of IVF0004488 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 68.6 bits (166), Expect = 2.4e-11
Identity = 81/373 (21.72%), Postives = 154/373 (41.29%), Query Frame = 0


Query: 352 KNMKVSLNGAENLILASDPDREGEAIAWHIIEMLQHEDALHEGISIARVVFHEITEASIK 411
           K ++     ++ L+L  D DREGE IA+ ++++ +   A+   + I R  F  + +  I 
Sbjct: 106 KTLEEEARKSDWLVLWLDCDREGENIAFEVVDVCR---AVKHNLFIRRAHFSALIDRDIH 165

Query: 412 SALQSPRVIDANLVQAYLARRALDYLIGFNIS---PLLWRKLPGCRSAGRVQS------- 471
            A+Q+ R  +    +A  AR+ +D  IG + +    +L R      S G  +S       
Sbjct: 166 EAVQNLRDPNQLFAEAVDARQEIDLRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGP 225

Query: 472 ---AALALICDREMEIDEFHAQEYWTIDIKLNQKDTCSSVEDFAVPAHLTHFDSKKLNQF 531
                L  I +R  EI     +E+WTI+     ++  ++     +  HL  + S  +   
Sbjct: 226 CQFPTLGFIVERYWEIQAHEPEEFWTINCSHQSEEGLATFN--WMRGHLFDYASAVI--- 285

Query: 532 AISSNMEAKNIESVLKSVNFQVLSSKKNKVR-RNPPMPYITSTLQQDAASKFNFSTTYTM 591
                      E  ++     V++    + R + PP P  T  L++ A+  F  S+ +TM
Sbjct: 286 ---------LYEMCVEEPTATVMNVPHPRERFKYPPYPLNTIELEKRASRYFRLSSEHTM 345

Query: 592 QVAQKLYEGIQLADGKAAGLITYLRTDGLHISDEAVKDIRSLIMQR--------YGKDFV 651
           +VA++LY+         AG I+Y RT+    S     D+R+++ ++        Y +  +
Sbjct: 346 KVAEELYQ---------AGFISYPRTETDSFSSRT--DLRAMVEEQTRHPAWGSYAQRLL 405

Query: 652 PESGPKYFKKVKNAQE--AHEAIRPTDVQRLPSMLVGILDEDSHKLYSLIWLRTVACQME 701
              G  +        +  AH  I PT      S        D   +Y L+    +AC  +
Sbjct: 406 EPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESN----WSRDHLNVYELVVRHYLACVSQ 446

The following BLAST results are available for this feature:

BLAST of IVF0004488 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1RIM1	1.0e-107	50.37	DNA topoisomerase 1 OS=Rickettsia bellii (strain RML369-C) OX=336407 GN=topA PE=...	[more]
Q4UM42	5.0e-107	49.88	DNA topoisomerase 1 OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315...	[more]
Q92IH1	2.1e-105	49.38	DNA topoisomerase 1 OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272...	[more]
Q68X45	3.6e-105	49.63	DNA topoisomerase 1 OS=Rickettsia typhi (strain ATCC VR-144 / Wilmington) OX=257...	[more]
Q9ZDK2	1.8e-104	48.64	DNA topoisomerase 1 OS=Rickettsia prowazekii (strain Madrid E) OX=272947 GN=topA...	[more]

BLAST of IVF0004488 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TAX8	0.0e+00	99.57	Omega-protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006510...	[more]
A0A5A7T7A4	0.0e+00	72.99	DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G00...	[more]
A0A1S4E6F1	0.0e+00	72.99	DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103503742 PE=3 SV=1	[more]
A0A1S4E5M8	0.0e+00	72.99	Omega-protein OS=Cucumis melo OX=3656 GN=LOC103503742 PE=3 SV=1	[more]
A0A6J1IF86	6.6e-296	74.77	Omega-protein OS=Cucurbita maxima OX=3661 GN=LOC111476696 PE=3 SV=1	[more]

BLAST of IVF0004488 vs. NCBI nr


Match Name	Identity	Description
KAA0038737.1	99.43	DNA topoisomerase 1 isoform X2 [Cucumis melo var. makuwa] >TYK31350.1 DNA topois...	[more]
XP_011652536.2	84.01	uncharacterized protein LOC101210581 isoform X1 [Cucumis sativus]	[more]
KAA0038738.1	72.76	DNA topoisomerase 1 isoform X1 [Cucumis melo var. makuwa] >TYK31351.1 DNA topois...	[more]
XP_016903546.1	72.76	PREDICTED: DNA topoisomerase 1 isoform X2 [Cucumis melo]	[more]
XP_016903545.1	72.76	PREDICTED: DNA topoisomerase 1 isoform X1 [Cucumis melo]	[more]

BLAST of IVF0004488 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31210.1	4.4e-167	57.45	DNA topoisomerase, type IA, core	[more]
AT5G63920.1	2.4e-11	21.72	topoisomerase 3alpha	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013497	DNA topoisomerase, type IA, central	PRINTS	PR00417	PRTPISMRASEI	coord: 364..377 score: 67.53 coord: 454..472 score: 56.05 coord: 673..689 score: 43.44 coord: 595..604 score: 66.73
IPR013497	DNA topoisomerase, type IA, central	PFAM	PF01131	Topoisom_bac	coord: 420..698 e-value: 1.1E-68 score: 232.1
IPR003602	DNA topoisomerase, type IA, DNA-binding domain	SMART	SM00437	topIaneu2	coord: 544..707 e-value: 7.9E-10 score: 32.9
IPR006171	TOPRIM domain	SMART	SM00493	toprim5	coord: 290..390 e-value: 9.8E-20 score: 81.6
IPR006171	TOPRIM domain	PFAM	PF01751	Toprim	coord: 292..405 e-value: 1.2E-21 score: 76.9
IPR006171	TOPRIM domain	PROSITE	PS50880	TOPRIM	coord: 290..405 score: 22.229134
IPR003601	DNA topoisomerase, type IA, domain 2	SMART	SM00436	topIban2	coord: 396..484 e-value: 3.5E-41 score: 152.8
IPR013824	DNA topoisomerase, type IA, central region, subdomain 1	GENE3D	1.10.460.10	Topoisomerase I, domain 2	coord: 430..475 e-value: 9.1E-91 score: 307.2
None	No IPR available	GENE3D	3.40.50.140		coord: 287..426 e-value: 4.5E-37 score: 129.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 206..261
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..172
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 173..187
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..193
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 206..243
None	No IPR available	PANTHER	PTHR42785:SF4	BNAA01G05990D PROTEIN	coord: 156..697
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..32 score: 5.0
IPR013826	DNA topoisomerase, type IA, central region, subdomain 3	GENE3D	1.10.290.10	Topoisomerase I, domain 4	coord: 552..688 e-value: 9.1E-91 score: 307.2
IPR013825	DNA topoisomerase, type IA, central region, subdomain 2	GENE3D	2.70.20.10	Topoisomerase I, domain 3	coord: 476..698 e-value: 9.1E-91 score: 307.2
IPR005733	DNA topoisomerase I, bacterial-type	TIGRFAM	TIGR01051	TIGR01051	coord: 293..698 e-value: 1.5E-139 score: 464.8
IPR000380	DNA topoisomerase, type IA	PANTHER	PTHR42785	DNA TOPOISOMERASE, TYPE IA, CORE	coord: 156..697
IPR023406	DNA topoisomerase, type IA, active site	PROSITE	PS00396	TOPOISOMERASE_I_PROK	coord: 581..604
IPR034149	DNA topoisomerase 1, TOPRIM domain	CDD	cd03363	TOPRIM_TopoIA_TopoI	coord: 290..417 e-value: 3.55186E-51 score: 171.975
IPR023405	DNA topoisomerase, type IA, core domain	SUPERFAMILY	56712	Prokaryotic type I DNA topoisomerase	coord: 290..698

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0004488.1	IVF0004488.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006265	DNA topological change
cellular_component	GO:0005694	chromosome
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003917	DNA topoisomerase type I (single strand cut, ATP-independent) activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003916	DNA topoisomerase activity