IVF0004458 (gene) Melon (IVF77) v1

Overview
NameIVF0004458
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionNitrile-specifier protein 5
Locationchr11: 13242234 .. 13243293 (-)
RNA-Seq ExpressionIVF0004458
SyntenyIVF0004458
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCCCACAAATGGAAAATGGATTAAGGTTAGTATCTTCACGTTATCATAATCATCAACCTATTCAATTCTTAATTCCATTTTTTCACAAATTTATTAAAAAATCTTTGCAGATTGATCAAAAAACGATAGGTCCAGGTGCAAGAAGCTCTCACGCCATTGCAATTGTAGACCAAAAGGCCTACTCCTTCGGCGGAGAATTCTCCCCCCGACTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCATGATCACATCTGGACAGTCGTCGCTGTCTCCGGCGACATCCCTCCACCGCGCGTTGGCGTCACAATGGCCTCCATCGGCCAAATCATTTACGTCTTCGGCGGTAGGGATACAGAGCACAAGGAACTAAATGAGCTCTACTCCTTTGACACATCGATTAACAAATGGACGCTCCTCTCTGGCGGCGAATCTGGACCACCGCATCGAAGCTACCACTCCACAACGGCGGACGACCACAACGTCTACATCTTTGGCGGATGCGGCAACTCCGGTCGCCTCAACGACTTGTGGGCTTACGATACGGTAGATGGAAAATGGATAGAGTATCCAGCAGCAGGGGAAGAGTTGAAGGGCCGAGGTGGACCAGGCCTAGCTGTGGCCCAAGATAAAATATGGGTAGTGTATGGATTTGCGGGTATGGAAATGGATGATGTTCACTATTTTGATCCGGACCAAAATAAATGGGCCCAAGTTGAGACAAGAGGGCAAAGGCCGACGGCTAGGAGCGTGTTTTGCACGTGCGCAATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATGTAGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGTTTACGTGTTGGACACGGAGGTGTTAGAGTGGAGTAGGGTGGACGATGGGCTGGGCGTTGAGGATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTAGGGGATATCTATTTTTTTGCTCCGTGTGAGTGA

mRNA sequence

ATGGCTCCCACAAATGGAAAATGGATTAAGATTGATCAAAAAACGATAGGTCCAGGTGCAAGAAGCTCTCACGCCATTGCAATTGTAGACCAAAAGGCCTACTCCTTCGGCGGAGAATTCTCCCCCCGACTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCATGATCACATCTGGACAGTCGTCGCTGTCTCCGGCGACATCCCTCCACCGCGCGTTGGCGTCACAATGGCCTCCATCGGCCAAATCATTTACGTCTTCGGCGGTAGGGATACAGAGCACAAGGAACTAAATGAGCTCTACTCCTTTGACACATCGATTAACAAATGGACGCTCCTCTCTGGCGGCGAATCTGGACCACCGCATCGAAGCTACCACTCCACAACGGCGGACGACCACAACGTCTACATCTTTGGCGGATGCGGCAACTCCGGTCGCCTCAACGACTTGTGGGCTTACGATACGGTAGATGGAAAATGGATAGAGTATCCAGCAGCAGGGGAAGAGTTGAAGGGCCGAGGTGGACCAGGCCTAGCTGTGGCCCAAGATAAAATATGGGTAGTGTATGGATTTGCGGGTATGGAAATGGATGATGTTCACTATTTTGATCCGGACCAAAATAAATGGGCCCAAGTTGAGACAAGAGGGCAAAGGCCGACGGCTAGGAGCGTGTTTTGCACGTGCGCAATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATGTAGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGTTTACGTGTTGGACACGGAGGTGTTAGAGTGGAGTAGGGTGGACGATGGGCTGGGCGTTGAGGATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTAGGGGATATCTATTTTTTTGCTCCGTGTGAGTGA

Coding sequence (CDS)

ATGGCTCCCACAAATGGAAAATGGATTAAGATTGATCAAAAAACGATAGGTCCAGGTGCAAGAAGCTCTCACGCCATTGCAATTGTAGACCAAAAGGCCTACTCCTTCGGCGGAGAATTCTCCCCCCGACTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCATGATCACATCTGGACAGTCGTCGCTGTCTCCGGCGACATCCCTCCACCGCGCGTTGGCGTCACAATGGCCTCCATCGGCCAAATCATTTACGTCTTCGGCGGTAGGGATACAGAGCACAAGGAACTAAATGAGCTCTACTCCTTTGACACATCGATTAACAAATGGACGCTCCTCTCTGGCGGCGAATCTGGACCACCGCATCGAAGCTACCACTCCACAACGGCGGACGACCACAACGTCTACATCTTTGGCGGATGCGGCAACTCCGGTCGCCTCAACGACTTGTGGGCTTACGATACGGTAGATGGAAAATGGATAGAGTATCCAGCAGCAGGGGAAGAGTTGAAGGGCCGAGGTGGACCAGGCCTAGCTGTGGCCCAAGATAAAATATGGGTAGTGTATGGATTTGCGGGTATGGAAATGGATGATGTTCACTATTTTGATCCGGACCAAAATAAATGGGCCCAAGTTGAGACAAGAGGGCAAAGGCCGACGGCTAGGAGCGTGTTTTGCACGTGCGCAATTGGGAAGTACGTGTATATCTACGGTGGGGAAGTGGACCCCAGTGATGTAGGGCACTTGGGGGCCGGAAAGTTCGCCGGAGAGGTTTACGTGTTGGACACGGAGGTGTTAGAGTGGAGTAGGGTGGACGATGGGCTGGGCGTTGAGGATCATCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAAGGGAGGGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCGCCGAGCAATGATCGGCTAGGGGATATCTATTTTTTTGCTCCGTGTGAGTGA

Protein sequence

MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVYGGNSPSNDRLGDIYFFAPCE
Homology
BLAST of IVF0004458 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.1e-101
Identity = 170/320 (53.12%), Postives = 215/320 (67.19%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           M P   KW+K+ QK  GPGARSSHA+ +V  K Y FGGE  P + +DN L+VFDL    W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           ++   +G+ P P  GV+M +IG  IYV+GGRD + +  N L+S+DT  N+W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
            P RSYHS   DD  VY+FGG    GR+N L AYD VD KW+EYPAAGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD    +W  VET G  P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E+ P  RGWCAF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of IVF0004458 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 349.0 bits (894), Expect = 5.9e-95
Identity = 158/316 (50.00%), Postives = 209/316 (66.14%), Query Frame = 0

Query: 6   GKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAV 65
           GKWIK++QK  GPG R SH IA V  K YSFGGEF+P  P+D  L+VFDL    W++   
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GD+P    +GV M S+G  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQD 185
           S+HS  AD+ NVY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R+D   G E+ P  RGW A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of IVF0004458 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.0e-94
Identity = 159/324 (49.07%), Postives = 213/324 (65.74%), Query Frame = 0

Query: 1   MAPT---NGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHD 60
           ++PT   +GKW K+D+   GPG R SH IA V  K YSFGGEF+P  P+D  L+VFD+  
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVVAVSGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSG 120
             W++   +GDIP    +GV M SIG  +YVFGGRD   ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GESGPPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGG 180
            E GP  RS+HS  AD+ NVY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKG 300
           I+GGE+    + H+G G+     + LDTE L+W R+D   G E+ P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLLVYGGNSPSNDRLGDIYFF 321
           G+ GL+++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

BLAST of IVF0004458 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-90
Identity = 154/316 (48.73%), Postives = 207/316 (65.51%), Query Frame = 0

Query: 6   GKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAV 65
           GKWIK++QK  GPG R SH IA V  K +SFGGE +P  P+D  L+VFDL    W++   
Sbjct: 152 GKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSISPA 211

Query: 66  SGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GD+P    +GV M SIG  +YVFGGRD   K  N  YSFDT+ N+W LL+  E GP  R
Sbjct: 212 TGDVPNLSCLGVRMVSIGSSLYVFGGRDASRK-YNGFYSFDTTKNEWKLLTPVEQGPTPR 271

Query: 126 SYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQD 185
           S+HS TAD++NVY+FGG   + RL  L AY+ VD KW++    G     RGG GL V Q 
Sbjct: 272 SFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVVQG 331

Query: 186 KIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVH +DP Q+KW QVET G++P ARSVF +  +GK++ ++GGE+  
Sbjct: 332 KVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEIAM 391

Query: 246 SDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVY 305
               H G G+ +G  + LDTE L+W ++D     E+ P  RGW A + G   G+ GL+++
Sbjct: 392 DPKAHEGPGQLSGGTFALDTETLKWEKLDKLGEEEETPSIRGWSASTTGTIDGKKGLVMF 451

Query: 306 GGNSPSNDRLGDIYFF 321
           GG + +NDR GD++F+
Sbjct: 452 GGKAQTNDRFGDLFFY 466

BLAST of IVF0004458 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.5e-87
Identity = 147/316 (46.52%), Postives = 198/316 (62.66%), Query Frame = 0

Query: 6   GKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAV 65
           G WIK++Q   GPG R SH IA V  K YSFGGE  P  P+D  L+VFDL    W++   
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GD+P    +GV M S+G  +Y FGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQD 185
           S+HS  AD+ NVY+FGG G   R+  L +Y+ VD  W      G+    RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+W+VYGF G E+DDVH++DP ++KW QVET G +P  RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVY 305
               H+G G+     + LDTE L+W R+D     E  P  RGW A + G   G+ GL+++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

BLAST of IVF0004458 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 3.5e-199
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

BLAST of IVF0004458 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 3.5e-199
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

BLAST of IVF0004458 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 659.4 bits (1700), Expect = 7.5e-186
Identity = 297/324 (91.67%), Postives = 310/324 (95.68%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPT G WIKIDQKT GPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLH+FDLHDHIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV A SGDIPPPRVGVTMAS+GQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGG+ G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PP RSYHSTTADD  +YIFGGCGNSGRLNDLWAYDT+DGKWIEYPAAGE LKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF P+QNKW QVET+G+RPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKF GEVYVLDTE LEWSRV+DGLG+EDHPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

BLAST of IVF0004458 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 284/322 (88.20%), Postives = 300/322 (93.17%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAP NGKW KIDQ   GPGARSSHAI IV +KAY+FGGEFSPR+PVDNKLHVFDL +H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTM ++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT+DGKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of IVF0004458 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 7.0e-176
Identity = 282/322 (87.58%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of IVF0004458 vs. NCBI nr
Match: XP_008464157.1 (PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >KAA0060815.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa] >TYK08820.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 698 bits (1802), Expect = 1.86e-254
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

BLAST of IVF0004458 vs. NCBI nr
Match: XP_004139996.1 (nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_004139998.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_011656888.1 nitrile-specifier protein 5 [Cucumis sativus] >KGN46612.1 hypothetical protein Csa_004873 [Cucumis sativus])

HSP 1 Score: 654 bits (1687), Expect = 6.37e-237
Identity = 297/324 (91.67%), Postives = 310/324 (95.68%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPT G WIKIDQKT GPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLH+FDLHDHIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV A SGDIPPPRVGVTMAS+GQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGG+ G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PP RSYHSTTADD  +YIFGGCGNSGRLNDLWAYDT+DGKWIEYPAAGE LKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF P+QNKW QVET+G+RPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKF GEVYVLDTE LEWSRV+DGLG+EDHPGPRGWCAF+VGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324
           LLVYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

BLAST of IVF0004458 vs. NCBI nr
Match: XP_038900567.1 (nitrile-specifier protein 5 [Benincasa hispida])

HSP 1 Score: 634 bits (1634), Expect = 7.65e-229
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAPTNGKW KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLHVFDLH+HIW
Sbjct: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV  VSGD+PPPRVGVTMAS+G+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFD  QN+WAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

BLAST of IVF0004458 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 624 bits (1608), Expect = 6.77e-225
Identity = 284/322 (88.20%), Postives = 300/322 (93.17%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAP NGKW KIDQ   GPGARSSHAI IV +KAY+FGGEFSPR+PVDNKLHVFDL +H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTM ++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT+DGKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGRKGGELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 322
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of IVF0004458 vs. NCBI nr
Match: XP_022947523.1 (nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier protein 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 621 bits (1601), Expect = 7.90e-224
Identity = 282/322 (87.58%), Postives = 301/322 (93.48%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           MAP NGKW KIDQ   GPGARSSHAI +V +KAY+FGGEFSPR+PVDN+LHVFDL +H W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           TV +VSGD+PPPRVGVTMA++GQI+YVFGGRD EHKELNELYSFDTSINKWTLLSGGESG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDT++GKWIEYP+AGE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFDP QNKWAQVET G+RPTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGW AFSVGRKGGELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 322
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

BLAST of IVF0004458 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 476.5 bits (1225), Expect = 1.7e-134
Identity = 207/320 (64.69%), Postives = 252/320 (78.75%), Query Frame = 0

Query: 3   PTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTV 62
           P  GKW+++ QK  GPGARSSHAIA+V  K Y+FGGEF PR+PVDN+L+VFDL    W++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  VAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPP 122
              SGD PPPRVGV MA++G IIY FGGRD+ H+ELNELY F+T  N+W LLS GE+GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 HRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVA 182
           +RSYHS TAD  NVY+FGGCG  GRLNDLWAY+ VD KWI++P+ GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEV 242
           Q KIWVVYGFAG E DDVH FD  + +W +VET+G++P+ARSVF T  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLL 302
           DPSD+GH+GAG F G+ Y LDTE LEW + +DG+G  +HPGPRGWCAF+ G + G+ GLL
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFAP 323
           VYGGNSPSNDRL DI+FF P
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

BLAST of IVF0004458 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 371.3 bits (952), Expect = 7.8e-103
Identity = 170/320 (53.12%), Postives = 215/320 (67.19%), Query Frame = 0

Query: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60
           M P   KW+K+ QK  GPGARSSHA+ +V  K Y FGGE  P + +DN L+VFDL    W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120
           ++   +G+ P P  GV+M +IG  IYV+GGRD + +  N L+S+DT  N+W LL+  E G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRD-DKRRYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180
            P RSYHS   DD  VY+FGG    GR+N L AYD VD KW+EYPAAGE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD    +W  VET G  P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300
           E +P ++ H+GAGK +GEVY LDTE L W R+  G   E+ P  RGWCAF+   K GE G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

BLAST of IVF0004458 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 349.0 bits (894), Expect = 4.2e-96
Identity = 158/316 (50.00%), Postives = 209/316 (66.14%), Query Frame = 0

Query: 6   GKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAV 65
           GKWIK++QK  GPG R SH IA V  K YSFGGEF+P  P+D  L+VFDL    W++   
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GD+P    +GV M S+G  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQD 185
           S+HS  AD+ NVY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R+D   G E+ P  RGW A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of IVF0004458 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 349.0 bits (894), Expect = 4.2e-96
Identity = 158/316 (50.00%), Postives = 209/316 (66.14%), Query Frame = 0

Query: 6   GKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIWTVVAV 65
           GKWIK++QK  GPG R SH IA V  K YSFGGEF+P  P+D  L+VFDL    W++   
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESGPPHR 125
           +GD+P    +GV M S+G  +YVFGGRD   ++ N  YSFDT+ N+W LL+  E GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLAVAQD 185
           S+HS  AD+ NVY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELGLLVY 305
             + H+G G+     + LDTE L+W R+D   G E+ P  RGW A +     G+ GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

BLAST of IVF0004458 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 348.2 bits (892), Expect = 7.1e-96
Identity = 159/324 (49.07%), Postives = 213/324 (65.74%), Query Frame = 0

Query: 1   MAPT---NGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHD 60
           ++PT   +GKW K+D+   GPG R SH IA V  K YSFGGEF+P  P+D  L+VFD+  
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVVAVSGDIPP-PRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSG 120
             W++   +GDIP    +GV M SIG  +YVFGGRD   ++ N  YSFDT+ N+W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDAS-RQYNGFYSFDTTTNEWKLLTP 411

Query: 121 GESGPPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGG 180
            E GP  RS+HS  AD+ NVY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+DP Q+KW QVET G RP+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKG 300
           I+GGE+    + H+G G+     + LDTE L+W R+D   G E+ P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 GELGLLVYGGNSPSNDRLGDIYFF 321
           G+ GL+++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93XW51.1e-10153.13Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1[more]
Q9SDM95.9e-9550.00Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2[more]
O043161.0e-9449.07Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1[more]
O493261.1e-9048.73Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1[more]
O043184.5e-8746.52Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CLA83.5e-199100.00nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1[more]
A0A5D3CBW13.5e-199100.00Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A0A0KDY97.5e-18691.67Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1[more]
A0A6J1I5Q11.1e-17688.20nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=... [more]
A0A6J1G6U47.0e-17687.58nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_008464157.11.86e-254100.00PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED:... [more]
XP_004139996.16.37e-23791.67nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier ... [more]
XP_038900567.17.65e-22988.89nitrile-specifier protein 5 [Benincasa hispida][more]
XP_022971053.16.77e-22588.20nitrile-specifier protein 5 [Cucurbita maxima][more]
XP_022947523.17.90e-22487.58nitrile-specifier protein 5 [Cucurbita moschata] >KAG6604919.1 Nitrile-specifier... [more]
Match NameE-valueIdentityDescription
AT3G07720.11.7e-13464.69Galactose oxidase/kelch repeat superfamily protein [more]
AT5G48180.17.8e-10353.13nitrile specifier protein 5 [more]
AT3G16400.14.2e-9650.00nitrile specifier protein 1 [more]
AT3G16400.24.2e-9650.00nitrile specifier protein 1 [more]
AT3G16410.17.1e-9649.07nitrile specifier protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006652Kelch repeat type 1SMARTSM00612kelc_smartcoord: 185..233
e-value: 3.3
score: 7.1
coord: 135..184
e-value: 0.33
score: 15.9
coord: 84..133
e-value: 4.0
score: 6.4
IPR006652Kelch repeat type 1PFAMPF01344Kelch_1coord: 72..114
e-value: 2.1E-8
score: 33.6
coord: 174..213
e-value: 7.8E-6
score: 25.4
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 197..324
e-value: 1.3E-15
score: 59.1
IPR015915Kelch-type beta propellerGENE3D2.120.10.80coord: 1..196
e-value: 1.8E-45
score: 157.2
IPR015915Kelch-type beta propellerSUPERFAMILY117281Kelch motifcoord: 3..241
NoneNo IPR availablePFAMPF13418Kelch_4coord: 123..161
e-value: 1.3E-12
score: 47.6
NoneNo IPR availablePANTHERPTHR47435KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)coord: 5..322
NoneNo IPR availablePANTHERPTHR47435:SF4KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)coord: 5..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0004458.2IVF0004458.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0080028 nitrile biosynthetic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0010043 response to zinc ion
cellular_component GO:0005634 nucleus
molecular_function GO:0030234 enzyme regulator activity
molecular_function GO:0005515 protein binding