IVF0004180 (gene) Melon (IVF77) v1

BLAST of IVF0004180 vs. ExPASy Swiss-Prot
Match: Q8W4D4 (BAG-associated GRAM protein 1 OS=Arabidopsis thaliana OX=3702 GN=BAGP1 PE=1 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.9e-216
Identity = 378/591 (63.96%), Postives = 467/591 (79.02%), Query Frame = 0

Query: 16  VIAMALDFLLPSWLEIKITLATSLFVVFAYWFL---------LTGVGY----LMLIVLWL 75
           ++  A++ LLPS  EI++ +  S+F++ +YW           + GVG+    LM  +   
Sbjct: 1   MVQSAVEVLLPSAWEIEVAVVASVFLIASYWLFAYRGGGDDDVVGVGFDRSRLMQNLDSG 60

Query: 76  MVLEMIPPGHLR-DLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGTEKRFSSMI 135
              +    GHLR D Q+N+AY++K+ELLAAKNLI ANLNGTSDPYAI+ CG+EKRFSSM+
Sbjct: 61  DAFDKDKIGHLRGDTQTNAAYIVKVELLAAKNLIGANLNGTSDPYAIVNCGSEKRFSSMV 120

Query: 136 PGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHTGAVWYTLD 195
           PGSRNPMWGEEFNF  DELP +I+ TI+DWDI+WKS VLGSVT+ VE EG TG VW++LD
Sbjct: 121 PGSRNPMWGEEFNFPTDELPAKINVTIHDWDIIWKSTVLGSVTINVEREGQTGPVWHSLD 180

Query: 196 STSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPLQTIFELLP 255
           S SGQVCL+I  IKLPVNA   V GYAGA  RRR++LD+   T+VHQKPGPLQTIF+LLP
Sbjct: 181 SPSGQVCLNINAIKLPVNAPRAVTGYAGA-GRRRVTLDQQGPTIVHQKPGPLQTIFDLLP 240

Query: 256 DEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRRTQHAFI 315
           DE+VEHS+SCALERSFLYHGRMYVS+WHICFHSN+FSKQMKVV+PLGDIDEIRR+QHA I
Sbjct: 241 DEVVEHSYSCALERSFLYHGRMYVSAWHICFHSNVFSKQMKVVVPLGDIDEIRRSQHALI 300

Query: 316 NPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREMLEAEKK 375
           NPA+TIILRMGAGGHGVPPLG+PDGRVRYKFASFWNRNH ++ALQR+VNN+  MLE EKK
Sbjct: 301 NPAITIILRMGAGGHGVPPLGTPDGRVRYKFASFWNRNHTLKALQRAVNNYHAMLEVEKK 360

Query: 376 EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPERF 435
           E+A+SALRAHSSSV+    K ++ A +   +   + QAF+KEEVL +I+N VF  +PE+ 
Sbjct: 361 ERAQSALRAHSSSVK-GGGKVQVKAPEDTAAVPVKFQAFIKEEVLVNIYNDVFASTPEQV 420

Query: 436 FSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPMCPPDTAMT 495
            + LL+D S YT+ + + RKD NL +  WH A+EY+GQVRE+ +RS+C+SPMCPPDTA+T
Sbjct: 421 LNVLLADDSTYTNEYRSARKDKNLNIEPWHTAEEYDGQVREIKFRSICNSPMCPPDTAVT 480

Query: 496 EYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIKAGVHFKKW 555
           E+QHVVLS DKK LVFETVQ  HDVPFG+ FE+HCRW LE   E SS +DI+ GVHFKKW
Sbjct: 481 EWQHVVLSPDKKVLVFETVQQPHDVPFGSYFEVHCRWRLEAKDETSSVIDIRVGVHFKKW 540

Query: 556 CLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGN---ETDKVA-SAPS 589
           CLMQSKIKAGA+ EYK+ V++ LEVAL Y+ S +S +   + DK A S+P+
Sbjct: 541 CLMQSKIKAGAIDEYKKEVEVMLEVALSYLKSHSSSSSHGDIDKSALSSPT 589

BLAST of IVF0004180 vs. ExPASy Swiss-Prot
Match: Q9ZVT9 (C2 and GRAM domain-containing protein At1g03370 OS=Arabidopsis thaliana OX=3702 GN=At1g03370 PE=2 SV=4)

HSP 1 Score: 101.3 bits (251), Expect = 3.9e-20
Identity = 116/499 (23.25%), Postives = 203/499 (40.68%), Query Frame = 0

Query: 82  YLIKLELLAAKNLIAANLNGTSDPYAIITCGTEKRFSSMIPGSRNPMWGEEFNF-SVDEL 141
           +L+ + L+   +L A + +G  DPY + T   + R SS+     NP W E F F ++ + 
Sbjct: 535 WLLTVALIEGVDLAAVDPSGHCDPYIVFTSNGKTRTSSIKFQKSNPQWNEIFEFDAMADP 594

Query: 142 PVQIHATIYDWDIVWKSAV-LGSVTVTV--ENEGHTGAVWYTLDSTSGQVC---LHIKTI 201
           P  ++  ++D+D  +  AV LG   V     N      VW  L     Q C   LH++  
Sbjct: 595 PSVLNVEVFDFDGPFDEAVSLGHAEVNFVRSNISDLADVWVPLQGKLAQACQSKLHLRIF 654

Query: 202 KLPVNAGSTVNGYAGANAR---RRISLDKPELTVVHQKPGPLQTIFELLPDEIVEHSFSC 261
                 G  V  Y     +   ++I++  P+     QK      +F L  +E + + F+C
Sbjct: 655 LDHTGGGDVVRDYLNKMEKEVGKKINVRSPQTNSAFQK------LFGLPQEEFLINDFTC 714

Query: 262 ALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIR---RTQHAFINPAVTII 321
            L+R     GR+++S+  + F+++IF  + K      DI+EI+    T  +  +P V + 
Sbjct: 715 HLKRKMPLQGRLFLSARIVGFYASIFGNKTKFFFLWEDIEEIQVLPPTLASMGSPIVVMT 774

Query: 322 L--------RMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREMLEAEK 381
           L        R+GA  H        +GR+++ F SF + N      Q+++    +      
Sbjct: 775 LRPNRGLDARIGAKTH------DEEGRLKFHFHSFVSFN----VAQKTIMALWKAKSLTP 834

Query: 382 KEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPER 441
           ++K ++        ++  ES   +  DD+  S+               + +   P  P  
Sbjct: 835 EQKVQAVEEESEQKLQSEESGLFLGVDDVRFSE---------------VFSLTLPV-PVS 894

Query: 442 FFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAA--DEYEGQVRELTYRSLCHSPMCPPDT 501
           FF  L   G     A + R    +     W +   D YE   R+  YR          D 
Sbjct: 895 FFMELFGGGEVDRKA-MERAGCQSYSCSPWESEKDDVYE---RQTYYR----------DK 954

Query: 502 AMTEYQHVVLSEDKKKLV-------FETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVD 549
            ++ Y+  V S  +K LV        E V   H VP G  F LH R+ +E++     +  
Sbjct: 955 RISRYRGEVTSTQQKSLVPEKNGWLVEEVMTLHGVPLGDYFNLHLRYQMEESTSKPKTTY 987

BLAST of IVF0004180 vs. ExPASy Swiss-Prot
Match: Q9FGS8 (C2 and GRAM domain-containing protein At5g50170 OS=Arabidopsis thaliana OX=3702 GN=At5g50170 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-14
Identity = 110/540 (20.37%), Postives = 224/540 (41.48%), Query Frame = 0

Query: 50   TGVGYLMLIVLWLMVLEMIPP----GHLRDLQSN-SAYLIKLELLAAKNLIAANLNGTSD 109
            +G+  L+L  +++M +  +      G  + +++N   +++ + L+   NL +       D
Sbjct: 502  SGILVLLLERVYIMTVHFVQARLHRGRDQGVKANGKGWILTIALIKGTNLASVEATELFD 561

Query: 110  PYAIITCGTEKRFSSMIPGSRNPMWGEEFNF-SVDELPVQIHATIYDWDIVW-KSAVLGS 169
            PY + TC  + R SS+   +++P W E   F +++E P  +   ++D+D  + + A LG 
Sbjct: 562  PYVVFTCNGKTRTSSVKLQAQDPQWNEVIEFDAMEEPPSVLDVEVFDFDGPFDQGASLGH 621

Query: 170  VTV-----TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAG---ANARR 229
              +     T +             + + Q  L ++      N   T+  Y         +
Sbjct: 622  AEINFLKHTADELADLSVALVGNHAQASQSKLQLRIFLENKNGVETMKDYLSKVEKEVGK 681

Query: 230  RISLDKPELTVVHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHS 289
            ++++  P      QK    Q +F L  +E +   ++C L+R     G++++S+  + F+S
Sbjct: 682  KLNIRSP------QKNSAFQKLFGLPHEEFLLKEYTCYLKRKLPVQGKLFLSARIVAFYS 741

Query: 290  NIFSKQMKVVIPLGDIDEIRRTQHAFI---NPAVTIILRMGAG---GHGVPPLGSPDGRV 349
            N+F  + K      DID+I+     F    +P + IIL+   G    HG       +GR+
Sbjct: 742  NVFGHKTKFYFLWEDIDDIQVLPPTFASLGSPLLLIILKKNRGLDAKHGAKS-QDDEGRL 801

Query: 350  RYKFASFWNRNHVVRALQR-------SVNNFREMLEAEKKEKAESALRAHSSSVRVSESK 409
             + F SF + +   R +         SV++  +++E E ++ A+  L   + +V VS+  
Sbjct: 802  SFYFQSFVSFDATSRTIMALWKTRTLSVDHRAQIVE-EDQDVADPFLLPEAVTV-VSD-- 861

Query: 410  EKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRK 469
                AD L  SK               ++    PC  E      +  G       + +  
Sbjct: 862  ----ADALMMSK---------------VYTCDLPCDVELVMK--IFGGGELERKIMEKSG 921

Query: 470  DSNLVMGQWHAADE--YEGQVRELTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFET 529
              +     W +     YE   R L+Y+   +  +      +T  Q    + + +  +   
Sbjct: 922  CLSYASTTWESKKPGVYE---RRLSYKYNHYVSVF--GGGVTCAQQKSPAPNDEGWILNE 981

Query: 530  VQNAHDVPFGANFELHCRWSLEKNAED--SSSVDIKAGVHFKKWCLMQSKIKAGAMLEYK 558
            +   HDVPFG +F +H R+ ++K   D  +S  ++   + + K    + +I    M +++
Sbjct: 982  IVALHDVPFGDHFRVHIRYEVKKAGVDCKTSKCEVYLKIRWLKTIKFEQRISKSIMEKFR 1004

BLAST of IVF0004180 vs. ExPASy Swiss-Prot
Match: F4HVW5 (Protein VASCULAR ASSOCIATED DEATH 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VAD1 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 5.4e-14
Identity = 93/393 (23.66%), Postives = 147/393 (37.40%), Query Frame = 0

Query: 226 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 285
           V  K    + +F L  DEI+   F+CA + S L  G MY+   +ICF+SNIF  + K +I
Sbjct: 65  VISKSEEYRQLFRLPADEILVQDFNCACQESILMQGHMYLFIHYICFYSNIFGYETKKII 124

Query: 286 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 345
           P  +I  ++R + A I P    IL   AGG             +Y FASF +R+   + +
Sbjct: 125 PFAEISCVKRAKTAGIFPNAIEIL---AGGK------------KYFFASFLSRDEAFKLI 184

Query: 346 Q-------RSVNNFREMLEAEKKEKAESALRAHSSSVRVSE------------------- 405
                    +V +  E+L  E +       RA SS    +E                   
Sbjct: 185 HDGWLEYGSAVKSEGEILVTEPQVSDGVVKRARSSMDLANELDIPVRDETLHLSSSSSLP 244

Query: 406 --SKEKIPADDLPKS---------------KNTQTQAFLKEEVLTSIHNGVFPCSPERFF 465
             S+  +P   + +                K   T A       T +    F    E FF
Sbjct: 245 VISQNGVPPSSVQRHAEPDVDVVAANTFNWKPEDTDAPKLSSDFTKVAEAKFSIPVEEFF 304

Query: 466 STLLSDGS-GYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPMCPPDTAMT 525
               SDG+  +  +F     D       W   ++  G  R ++++               
Sbjct: 305 RLFFSDGAVSFVESFHKNCGDKEFRCTSWQPHEKL-GHTRNVSFQHPIKIYFGAKFGGCQ 364

Query: 526 EYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSS---VDIKAGVHF 572
           E Q   +  +   LV ET Q   DVP+   F +   W L+++  DS     +D+   V F
Sbjct: 365 ESQKFRMYRN-SHLVIETSQEISDVPYADYFTVEGVWDLKRDCRDSVEGCILDVYVNVAF 424

BLAST of IVF0004180 vs. ExPASy Swiss-Prot
Match: O42976 (Uncharacterized membrane protein C20F10.07 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC20F10.07 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 3.1e-09
Identity = 43/138 (31.16%), Postives = 67/138 (48.55%), Query Frame = 0

Query: 204 VNGYAGANARRRISLDKPELTVVHQKPGPLQTIFELLP--DEIVEHSFSCALERSFLYHG 263
           ++GYA AN++R     +               IF++LP  D +++  + CAL+R    HG
Sbjct: 185 LSGYAFANSKRNRDFHR---------------IFKVLPPEDHLID-DYGCALQRDIFLHG 244

Query: 264 RMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPL 323
           RMY+S  HICF+S+IF     +VIP+ +I  + +   A + P    I  + A        
Sbjct: 245 RMYLSESHICFNSSIFGWVTNIVIPVTEIVSVEKKSTAVVFPNAIQITTLHA-------- 291

Query: 324 GSPDGRVRYKFASFWNRN 340
                  RY FASF +R+
Sbjct: 305 -------RYIFASFISRD 291

BLAST of IVF0004180 vs. ExPASy TrEMBL
Match: A0A1S3BXU5 (BAG-associated GRAM protein 1 OS=Cucumis melo OX=3656 GN=LOC103494589 PE=4 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 575/600 (95.83%), Postives = 578/600 (96.33%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLLTGVGYL---ML 60
           MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF     G       
Sbjct: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFFAYWSGLFDADRS 60

Query: 61  IV----LWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120
           +V      + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT
Sbjct: 61  VVDGSGDGIHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120

Query: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHT 180
           EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTVTVENEGHT
Sbjct: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHVTIYDWDIVWKSAVLGSVTVTVENEGHT 180

Query: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240
           GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL
Sbjct: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240

Query: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300
           QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI
Sbjct: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300

Query: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360
           RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR
Sbjct: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360

Query: 361 EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV 420
           EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV
Sbjct: 361 EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV 420

Query: 421 FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480
           FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM
Sbjct: 421 FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480

Query: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540
           CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK
Sbjct: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540

Query: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 594
           AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS
Sbjct: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 600

BLAST of IVF0004180 vs. ExPASy TrEMBL
Match: A0A5A7TNV3 (BAG-associated GRAM protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G002960 PE=4 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 574/604 (95.03%), Postives = 578/604 (95.70%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLLTGVGYL---ML 60
           MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF     G       
Sbjct: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFFAYWSGLFDADRS 60

Query: 61  IV----LWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120
           +V      + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT
Sbjct: 61  VVDGSGDGIHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120

Query: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHT 180
           EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTVTVENEGHT
Sbjct: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHVTIYDWDIVWKSAVLGSVTVTVENEGHT 180

Query: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240
           GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL
Sbjct: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240

Query: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300
           QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI
Sbjct: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300

Query: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360
           RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDG+VRYKFASFWNRNHVVRALQRSVNNFR
Sbjct: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGQVRYKFASFWNRNHVVRALQRSVNNFR 360

Query: 361 EMLEAEKK----EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSI 420
           EMLEAEKK    EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSI
Sbjct: 361 EMLEAEKKACSLEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSI 420

Query: 421 HNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLC 480
           HNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLC
Sbjct: 421 HNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLC 480

Query: 481 HSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSS 540
           HSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSS
Sbjct: 481 HSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSS 540

Query: 541 VDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSE 594
           VDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSE
Sbjct: 541 VDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSE 600

BLAST of IVF0004180 vs. ExPASy TrEMBL
Match: A0A0A0KTV0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011670 PE=4 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 562/600 (93.67%), Postives = 571/600 (95.17%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLLTGVGYL---ML 60
           M+SSILLHFEFRPQMV+AMALDFLLPSWLEIKITLATSLFV+FAYWF     G       
Sbjct: 1   MSSSILLHFEFRPQMVMAMALDFLLPSWLEIKITLATSLFVIFAYWFFAYWSGLFDADRS 60

Query: 61  IV----LWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120
           +V      + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT
Sbjct: 61  VVDGSGDGIHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120

Query: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHT 180
           EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTVTVENEGHT
Sbjct: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHITIYDWDIVWKSAVLGSVTVTVENEGHT 180

Query: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240
           GAVWYTLDS SGQVCLHIKTIKLPVNAGS VNGYAGAN RRRISLDKPELTVVHQKPG L
Sbjct: 181 GAVWYTLDSPSGQVCLHIKTIKLPVNAGSPVNGYAGANPRRRISLDKPELTVVHQKPGAL 240

Query: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300
           QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI
Sbjct: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300

Query: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360
           RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR
Sbjct: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360

Query: 361 EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV 420
           EMLEAEKKEKAESALRAHSSSVRVSE+KEKIPADDLPKSKN  TQAFLKEEVLTSIHNGV
Sbjct: 361 EMLEAEKKEKAESALRAHSSSVRVSETKEKIPADDLPKSKN--TQAFLKEEVLTSIHNGV 420

Query: 421 FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480
           FPCSPERFFSTLLSDGSGYTSAFVA+RKD+NLVMGQWHAADEYEGQVRELTYRSLCHSPM
Sbjct: 421 FPCSPERFFSTLLSDGSGYTSAFVAKRKDTNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480

Query: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540
           CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK
Sbjct: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540

Query: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 594
           AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNS TSGNETDKVASAPSEAQSS
Sbjct: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSNTSGNETDKVASAPSEAQSS 598

BLAST of IVF0004180 vs. ExPASy TrEMBL
Match: A0A6J1EX96 (BAG-associated GRAM protein 1 OS=Cucurbita moschata OX=3662 GN=LOC111439209 PE=4 SV=1)

HSP 1 Score: 1022.3 bits (2642), Expect = 8.0e-295
Identity = 517/600 (86.17%), Postives = 545/600 (90.83%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF------LLTG-VG 60
           MTSSILLH EFRPQMVI MALDFLLPSWLEIKITLATSLFV+FAYWF      L  G   
Sbjct: 1   MTSSILLHSEFRPQMVIPMALDFLLPSWLEIKITLATSLFVIFAYWFFAYRSSLFDGDPS 60

Query: 61  YLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120
            L      +   + IPPGHLRDLQ+NSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT
Sbjct: 61  VLDNSGDSIDAKDKIPPGHLRDLQTNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120

Query: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHT 180
           EKRFSSMIPGSRNPMWGEEF+FSVDELP QI+ TIYDWDIVWKSAVLGSVTVTVE+EGHT
Sbjct: 121 EKRFSSMIPGSRNPMWGEEFDFSVDELPAQINVTIYDWDIVWKSAVLGSVTVTVESEGHT 180

Query: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240
           GAVWYTLDSTSGQVCLHIKT+KL  N GS +NGYAGANARRRISLDKPELTVVHQKPGPL
Sbjct: 181 GAVWYTLDSTSGQVCLHIKTLKLQGNGGSAINGYAGANARRRISLDKPELTVVHQKPGPL 240

Query: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300
           QTIFELLPDE+VEHSFSCALERSFLYHGRMYVS+WHICFHSNIFSKQMKV+IPL DIDE+
Sbjct: 241 QTIFELLPDEVVEHSFSCALERSFLYHGRMYVSAWHICFHSNIFSKQMKVIIPLEDIDEV 300

Query: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360
           RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNH VRALQR+V NFR
Sbjct: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHTVRALQRAVKNFR 360

Query: 361 EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV 420
           E LEAEKKEKAESALRAHSSSVRVSES EKI AD+LPK+KN  TQAF+KEEVLTSIHNGV
Sbjct: 361 ETLEAEKKEKAESALRAHSSSVRVSESTEKIAADNLPKNKN--TQAFIKEEVLTSIHNGV 420

Query: 421 FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480
           FPC+PE+FF+ LLSDGS YTSAFVARRKD+NLVMGQWHAADEYEGQVRELTYRSLCHSPM
Sbjct: 421 FPCTPEQFFTILLSDGSNYTSAFVARRKDTNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480

Query: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540
           CPPDTAMTEYQH VLSEDK KLVFE VQNAHDVPFGA FELHCRWSLE NAE+SSS++IK
Sbjct: 481 CPPDTAMTEYQHAVLSEDKTKLVFEMVQNAHDVPFGAYFELHCRWSLETNAENSSSIEIK 540

Query: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 594
           AGVHFKKWCLMQSKIKAGAMLEYKRAV+LRLEVALEY  S   G+E DK+A  P  AQS+
Sbjct: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVELRLEVALEYTKSNAPGSENDKIAPPPPPAQST 598

BLAST of IVF0004180 vs. ExPASy TrEMBL
Match: A0A6J1I8V7 (BAG-associated GRAM protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111470728 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.7e-292
Identity = 513/600 (85.50%), Postives = 544/600 (90.67%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF------LLTG-VG 60
           MTSSILL+ EFRPQMVI MALDFLLPSWLEIKITLATSLFV+FAYWF      L  G   
Sbjct: 1   MTSSILLYSEFRPQMVIPMALDFLLPSWLEIKITLATSLFVIFAYWFFAYRSSLFDGDPS 60

Query: 61  YLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120
            L      +   + I  GHLRDLQ+NSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT
Sbjct: 61  VLDNSGDSIDAKDKILTGHLRDLQTNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGT 120

Query: 121 EKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHT 180
           EKRFSSMIPGSRNPMWGEEF+FSVDELP QI+ TIYDWDIVWKSAVLGSVTVTVE+EGHT
Sbjct: 121 EKRFSSMIPGSRNPMWGEEFDFSVDELPAQINVTIYDWDIVWKSAVLGSVTVTVESEGHT 180

Query: 181 GAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPL 240
           GAVWYTLDSTSGQVCLHIKT+KL  N GS +NGYAGANARRRISLDKPELTVVHQKPGPL
Sbjct: 181 GAVWYTLDSTSGQVCLHIKTLKLQGNGGSAINGYAGANARRRISLDKPELTVVHQKPGPL 240

Query: 241 QTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEI 300
           QTIFELLPDE+VEHSFSCALERSFLYHGRMYVS+WHICFHSNIFSKQMKV+IPL DIDE+
Sbjct: 241 QTIFELLPDEVVEHSFSCALERSFLYHGRMYVSAWHICFHSNIFSKQMKVIIPLEDIDEV 300

Query: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFR 360
           RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNH VRALQR+V NFR
Sbjct: 301 RRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHTVRALQRAVKNFR 360

Query: 361 EMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGV 420
           E LEAEKKEKAESALRAHSSSVRVSES EKI AD+LPK+KN  TQAF+KEEVLTS+HNGV
Sbjct: 361 ETLEAEKKEKAESALRAHSSSVRVSESTEKIAADNLPKNKN--TQAFIKEEVLTSVHNGV 420

Query: 421 FPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480
           FPC+PE+FF+ LLSDGS YTSAFVARRKD+NLVMGQWHAADEYEGQVRELTYRSLCHSPM
Sbjct: 421 FPCTPEQFFTILLSDGSNYTSAFVARRKDTNLVMGQWHAADEYEGQVRELTYRSLCHSPM 480

Query: 481 CPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIK 540
           CPPDTAMTEYQH VLSEDK KLVFE VQNAHDVPFGA FELHCRWSLE NAE+SSS++I+
Sbjct: 481 CPPDTAMTEYQHAVLSEDKTKLVFEMVQNAHDVPFGAYFELHCRWSLETNAENSSSIEIR 540

Query: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 594
           AGVHFKKWCLMQSKIKAGAMLEYKRAV+LRLEVALEY  S  SG+E DK+A  P  AQS+
Sbjct: 541 AGVHFKKWCLMQSKIKAGAMLEYKRAVELRLEVALEYTKSNASGSENDKIAPPPPPAQST 598

BLAST of IVF0004180 vs. NCBI nr
Match: XP_008454047.1 (PREDICTED: BAG-associated GRAM protein 1 [Cucumis melo] >XP_008454048.1 PREDICTED: BAG-associated GRAM protein 1 [Cucumis melo])

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 575/608 (94.57%), Postives = 578/608 (95.07%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLL----------- 60
           MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF             
Sbjct: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFFAYWSGLFDADRS 60

Query: 61  ----TGVGYLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120
               +G G        + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP
Sbjct: 61  VVDGSGDG--------IHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120

Query: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTV 180
           YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTV
Sbjct: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHVTIYDWDIVWKSAVLGSVTV 180

Query: 181 TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV 240
           TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV
Sbjct: 181 TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV 240

Query: 241 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300
           VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI
Sbjct: 241 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300

Query: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 360
           PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL
Sbjct: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 360

Query: 361 QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEV 420
           QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEV
Sbjct: 361 QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEV 420

Query: 421 LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTY 480
           LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTY
Sbjct: 421 LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTY 480

Query: 481 RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE 540
           RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE
Sbjct: 481 RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE 540

Query: 541 DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVAS 593
           DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVAS
Sbjct: 541 DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVAS 600

BLAST of IVF0004180 vs. NCBI nr
Match: KAA0044548.1 (BAG-associated GRAM protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1118 bits (2893), Expect = 0.0
Identity = 574/612 (93.79%), Postives = 578/612 (94.44%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLL----------- 60
           MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF             
Sbjct: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFFAYWSGLFDADRS 60

Query: 61  ----TGVGYLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120
               +G G        + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP
Sbjct: 61  VVDGSGDG--------IHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120

Query: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTV 180
           YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTV
Sbjct: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHVTIYDWDIVWKSAVLGSVTV 180

Query: 181 TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV 240
           TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV
Sbjct: 181 TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV 240

Query: 241 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300
           VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI
Sbjct: 241 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300

Query: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 360
           PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDG+VRYKFASFWNRNHVVRAL
Sbjct: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGQVRYKFASFWNRNHVVRAL 360

Query: 361 QRSVNNFREMLEAEKK----EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFL 420
           QRSVNNFREMLEAEKK    EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFL
Sbjct: 361 QRSVNNFREMLEAEKKACSLEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFL 420

Query: 421 KEEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVR 480
           KEEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVR
Sbjct: 421 KEEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVR 480

Query: 481 ELTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLE 540
           ELTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLE
Sbjct: 481 ELTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLE 540

Query: 541 KNAEDSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETD 593
           KNAEDSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETD
Sbjct: 541 KNAEDSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETD 600

BLAST of IVF0004180 vs. NCBI nr
Match: KGN53020.1 (hypothetical protein Csa_014709 [Cucumis sativus])

HSP 1 Score: 1098 bits (2839), Expect = 0.0
Identity = 562/608 (92.43%), Postives = 571/608 (93.91%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLL----------- 60
           M+SSILLHFEFRPQMV+AMALDFLLPSWLEIKITLATSLFV+FAYWF             
Sbjct: 1   MSSSILLHFEFRPQMVMAMALDFLLPSWLEIKITLATSLFVIFAYWFFAYWSGLFDADRS 60

Query: 61  ----TGVGYLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120
               +G G        + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP
Sbjct: 61  VVDGSGDG--------IHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDP 120

Query: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTV 180
           YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTV
Sbjct: 121 YAIITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHITIYDWDIVWKSAVLGSVTV 180

Query: 181 TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTV 240
           TVENEGHTGAVWYTLDS SGQVCLHIKTIKLPVNAGS VNGYAGAN RRRISLDKPELTV
Sbjct: 181 TVENEGHTGAVWYTLDSPSGQVCLHIKTIKLPVNAGSPVNGYAGANPRRRISLDKPELTV 240

Query: 241 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300
           VHQKPG LQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI
Sbjct: 241 VHQKPGALQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 300

Query: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 360
           PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL
Sbjct: 301 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 360

Query: 361 QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEV 420
           QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSE+KEKIPADDLPKSKNTQ  AFLKEEV
Sbjct: 361 QRSVNNFREMLEAEKKEKAESALRAHSSSVRVSETKEKIPADDLPKSKNTQ--AFLKEEV 420

Query: 421 LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTY 480
           LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVA+RKD+NLVMGQWHAADEYEGQVRELTY
Sbjct: 421 LTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVAKRKDTNLVMGQWHAADEYEGQVRELTY 480

Query: 481 RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE 540
           RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE
Sbjct: 481 RSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAE 540

Query: 541 DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVAS 593
           DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNS TSGNETDKVAS
Sbjct: 541 DSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSNTSGNETDKVAS 598

BLAST of IVF0004180 vs. NCBI nr
Match: XP_031739869.1 (BAG-associated GRAM protein 1 [Cucumis sativus])

HSP 1 Score: 1081 bits (2796), Expect = 0.0
Identity = 553/598 (92.47%), Postives = 561/598 (93.81%), Query Frame = 0

Query: 11  FRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWFLL---------------TGVGYL 70
           FRPQMV+AMALDFLLPSWLEIKITLATSLFV+FAYWF                 +G G  
Sbjct: 11  FRPQMVMAMALDFLLPSWLEIKITLATSLFVIFAYWFFAYWSGLFDADRSVVDGSGDG-- 70

Query: 71  MLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGTEK 130
                 + V + IPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGTEK
Sbjct: 71  ------IHVKDKIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGTEK 130

Query: 131 RFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHTGA 190
           RFSSMIPGSRNPMWGEEFNFSVDELPVQIH TIYDWDIVWKSAVLGSVTVTVENEGHTGA
Sbjct: 131 RFSSMIPGSRNPMWGEEFNFSVDELPVQIHITIYDWDIVWKSAVLGSVTVTVENEGHTGA 190

Query: 191 VWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPLQT 250
           VWYTLDS SGQVCLHIKTIKLPVNAGS VNGYAGAN RRRISLDKPELTVVHQKPG LQT
Sbjct: 191 VWYTLDSPSGQVCLHIKTIKLPVNAGSPVNGYAGANPRRRISLDKPELTVVHQKPGALQT 250

Query: 251 IFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRR 310
           IFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRR
Sbjct: 251 IFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRR 310

Query: 311 TQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREM 370
           TQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREM
Sbjct: 311 TQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREM 370

Query: 371 LEAEKKEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFP 430
           LEAEKKEKAESALRAHSSSVRVSE+KEKIPADDLPKSKNTQ  AFLKEEVLTSIHNGVFP
Sbjct: 371 LEAEKKEKAESALRAHSSSVRVSETKEKIPADDLPKSKNTQ--AFLKEEVLTSIHNGVFP 430

Query: 431 CSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPMCP 490
           CSPERFFSTLLSDGSGYTSAFVA+RKD+NLVMGQWHAADEYEGQVRELTYRSLCHSPMCP
Sbjct: 431 CSPERFFSTLLSDGSGYTSAFVAKRKDTNLVMGQWHAADEYEGQVRELTYRSLCHSPMCP 490

Query: 491 PDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIKAG 550
           PDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIKAG
Sbjct: 491 PDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIKAG 550

Query: 551 VHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDKVASAPSEAQSS 593
           VHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNS TSGNETDKVASAPSEAQSS
Sbjct: 551 VHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSNTSGNETDKVASAPSEAQSS 598

BLAST of IVF0004180 vs. NCBI nr
Match: XP_038893958.1 (BAG-associated GRAM protein 1 [Benincasa hispida])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 545/610 (89.34%), Postives = 564/610 (92.46%), Query Frame = 0

Query: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIKITLATSLFVVFAYWF------------- 60
           MTSSILLHFEFRPQMVIAMALDFLLPSWLEI+ITLATSLFV+ AYWF             
Sbjct: 1   MTSSILLHFEFRPQMVIAMALDFLLPSWLEIEITLATSLFVILAYWFFAHWSGLFDADRS 60

Query: 61  LLTGVGYLMLIVLWLMVLEMIPPGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYA 120
           L+ G G        + V + IPPGHLRDLQ+NSAYLIKLELLAAKNLIAANLNGTSDPYA
Sbjct: 61  LIDGSGD------GIHVKDKIPPGHLRDLQTNSAYLIKLELLAAKNLIAANLNGTSDPYA 120

Query: 121 IITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTV 180
           IITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQI+ TIYDWDIVWKSAVLGSVTVTV
Sbjct: 121 IITCGTEKRFSSMIPGSRNPMWGEEFNFSVDELPVQINVTIYDWDIVWKSAVLGSVTVTV 180

Query: 181 ENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVH 240
           E+EGHTGAVWY+LDS SGQVCLHIKTIKLPVNAGSTVN YAGANARRRISLDKPELTVVH
Sbjct: 181 ESEGHTGAVWYSLDSPSGQVCLHIKTIKLPVNAGSTVNAYAGANARRRISLDKPELTVVH 240

Query: 241 QKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPL 300
           QKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPL
Sbjct: 241 QKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPL 300

Query: 301 GDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQR 360
           GDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQR
Sbjct: 301 GDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQR 360

Query: 361 SVNNFREMLEAEKKEKAESALRAHSSSVRVSESKEKI-----PADDLPKSKNTQTQAFLK 420
           SV NFREMLEAEKKEKAESALRAHSSSVRVSESKEKI     P D LPKSKNTQ  AFLK
Sbjct: 361 SVKNFREMLEAEKKEKAESALRAHSSSVRVSESKEKITADHLPNDHLPKSKNTQ--AFLK 420

Query: 421 EEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRE 480
           EEVL SIHNGVFPCSPE+FF+TLLSDGSGYTSAFV RRKD+NLVMGQWHAADEYEGQVRE
Sbjct: 421 EEVLASIHNGVFPCSPEQFFTTLLSDGSGYTSAFVLRRKDTNLVMGQWHAADEYEGQVRE 480

Query: 481 LTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEK 540
           LTYRSLCHSPMCPPDTAMTEYQHVVLSEDKK LVFETVQNAHDVPFGANFELHCRWSLEK
Sbjct: 481 LTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKNLVFETVQNAHDVPFGANFELHCRWSLEK 540

Query: 541 NAEDSSSVDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGNETDK 591
           NAEDSSS+DIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLR+EVAL+YMNS +SG+ T+K
Sbjct: 541 NAEDSSSIDIKAGVHFKKWCLMQSKIKAGAMLEYKRAVDLRIEVALDYMNSNSSGSVTEK 600

BLAST of IVF0004180 vs. TAIR 10
Match: AT3G59660.1 (C2 domain-containing protein / GRAM domain-containing protein )

HSP 1 Score: 753.4 bits (1944), Expect = 1.3e-217
Identity = 378/591 (63.96%), Postives = 467/591 (79.02%), Query Frame = 0

Query: 16  VIAMALDFLLPSWLEIKITLATSLFVVFAYWFL---------LTGVGY----LMLIVLWL 75
           ++  A++ LLPS  EI++ +  S+F++ +YW           + GVG+    LM  +   
Sbjct: 1   MVQSAVEVLLPSAWEIEVAVVASVFLIASYWLFAYRGGGDDDVVGVGFDRSRLMQNLDSG 60

Query: 76  MVLEMIPPGHLR-DLQSNSAYLIKLELLAAKNLIAANLNGTSDPYAIITCGTEKRFSSMI 135
              +    GHLR D Q+N+AY++K+ELLAAKNLI ANLNGTSDPYAI+ CG+EKRFSSM+
Sbjct: 61  DAFDKDKIGHLRGDTQTNAAYIVKVELLAAKNLIGANLNGTSDPYAIVNCGSEKRFSSMV 120

Query: 136 PGSRNPMWGEEFNFSVDELPVQIHATIYDWDIVWKSAVLGSVTVTVENEGHTGAVWYTLD 195
           PGSRNPMWGEEFNF  DELP +I+ TI+DWDI+WKS VLGSVT+ VE EG TG VW++LD
Sbjct: 121 PGSRNPMWGEEFNFPTDELPAKINVTIHDWDIIWKSTVLGSVTINVEREGQTGPVWHSLD 180

Query: 196 STSGQVCLHIKTIKLPVNAGSTVNGYAGANARRRISLDKPELTVVHQKPGPLQTIFELLP 255
           S SGQVCL+I  IKLPVNA   V GYAGA  RRR++LD+   T+VHQKPGPLQTIF+LLP
Sbjct: 181 SPSGQVCLNINAIKLPVNAPRAVTGYAGA-GRRRVTLDQQGPTIVHQKPGPLQTIFDLLP 240

Query: 256 DEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIRRTQHAFI 315
           DE+VEHS+SCALERSFLYHGRMYVS+WHICFHSN+FSKQMKVV+PLGDIDEIRR+QHA I
Sbjct: 241 DEVVEHSYSCALERSFLYHGRMYVSAWHICFHSNVFSKQMKVVVPLGDIDEIRRSQHALI 300

Query: 316 NPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREMLEAEKK 375
           NPA+TIILRMGAGGHGVPPLG+PDGRVRYKFASFWNRNH ++ALQR+VNN+  MLE EKK
Sbjct: 301 NPAITIILRMGAGGHGVPPLGTPDGRVRYKFASFWNRNHTLKALQRAVNNYHAMLEVEKK 360

Query: 376 EKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPERF 435
           E+A+SALRAHSSSV+    K ++ A +   +   + QAF+KEEVL +I+N VF  +PE+ 
Sbjct: 361 ERAQSALRAHSSSVK-GGGKVQVKAPEDTAAVPVKFQAFIKEEVLVNIYNDVFASTPEQV 420

Query: 436 FSTLLSDGSGYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPMCPPDTAMT 495
            + LL+D S YT+ + + RKD NL +  WH A+EY+GQVRE+ +RS+C+SPMCPPDTA+T
Sbjct: 421 LNVLLADDSTYTNEYRSARKDKNLNIEPWHTAEEYDGQVREIKFRSICNSPMCPPDTAVT 480

Query: 496 EYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVDIKAGVHFKKW 555
           E+QHVVLS DKK LVFETVQ  HDVPFG+ FE+HCRW LE   E SS +DI+ GVHFKKW
Sbjct: 481 EWQHVVLSPDKKVLVFETVQQPHDVPFGSYFEVHCRWRLEAKDETSSVIDIRVGVHFKKW 540

Query: 556 CLMQSKIKAGAMLEYKRAVDLRLEVALEYMNSITSGN---ETDKVA-SAPS 589
           CLMQSKIKAGA+ EYK+ V++ LEVAL Y+ S +S +   + DK A S+P+
Sbjct: 541 CLMQSKIKAGAIDEYKKEVEVMLEVALSYLKSHSSSSSHGDIDKSALSSPT 589

BLAST of IVF0004180 vs. TAIR 10
Match: AT1G03370.1 (C2 calcium/lipid-binding and GRAM domain containing protein )

HSP 1 Score: 101.3 bits (251), Expect = 2.8e-21
Identity = 116/499 (23.25%), Postives = 203/499 (40.68%), Query Frame = 0

Query: 82  YLIKLELLAAKNLIAANLNGTSDPYAIITCGTEKRFSSMIPGSRNPMWGEEFNF-SVDEL 141
           +L+ + L+   +L A + +G  DPY + T   + R SS+     NP W E F F ++ + 
Sbjct: 535 WLLTVALIEGVDLAAVDPSGHCDPYIVFTSNGKTRTSSIKFQKSNPQWNEIFEFDAMADP 594

Query: 142 PVQIHATIYDWDIVWKSAV-LGSVTVTV--ENEGHTGAVWYTLDSTSGQVC---LHIKTI 201
           P  ++  ++D+D  +  AV LG   V     N      VW  L     Q C   LH++  
Sbjct: 595 PSVLNVEVFDFDGPFDEAVSLGHAEVNFVRSNISDLADVWVPLQGKLAQACQSKLHLRIF 654

Query: 202 KLPVNAGSTVNGYAGANAR---RRISLDKPELTVVHQKPGPLQTIFELLPDEIVEHSFSC 261
                 G  V  Y     +   ++I++  P+     QK      +F L  +E + + F+C
Sbjct: 655 LDHTGGGDVVRDYLNKMEKEVGKKINVRSPQTNSAFQK------LFGLPQEEFLINDFTC 714

Query: 262 ALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVIPLGDIDEIR---RTQHAFINPAVTII 321
            L+R     GR+++S+  + F+++IF  + K      DI+EI+    T  +  +P V + 
Sbjct: 715 HLKRKMPLQGRLFLSARIVGFYASIFGNKTKFFFLWEDIEEIQVLPPTLASMGSPIVVMT 774

Query: 322 L--------RMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRALQRSVNNFREMLEAEK 381
           L        R+GA  H        +GR+++ F SF + N      Q+++    +      
Sbjct: 775 LRPNRGLDARIGAKTH------DEEGRLKFHFHSFVSFN----VAQKTIMALWKAKSLTP 834

Query: 382 KEKAESALRAHSSSVRVSESKEKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPER 441
           ++K ++        ++  ES   +  DD+  S+               + +   P  P  
Sbjct: 835 EQKVQAVEEESEQKLQSEESGLFLGVDDVRFSE---------------VFSLTLPV-PVS 894

Query: 442 FFSTLLSDGSGYTSAFVARRKDSNLVMGQWHAA--DEYEGQVRELTYRSLCHSPMCPPDT 501
           FF  L   G     A + R    +     W +   D YE   R+  YR          D 
Sbjct: 895 FFMELFGGGEVDRKA-MERAGCQSYSCSPWESEKDDVYE---RQTYYR----------DK 954

Query: 502 AMTEYQHVVLSEDKKKLV-------FETVQNAHDVPFGANFELHCRWSLEKNAEDSSSVD 549
            ++ Y+  V S  +K LV        E V   H VP G  F LH R+ +E++     +  
Sbjct: 955 RISRYRGEVTSTQQKSLVPEKNGWLVEEVMTLHGVPLGDYFNLHLRYQMEESTSKPKTTY 987

BLAST of IVF0004180 vs. TAIR 10
Match: AT5G50170.1 (C2 calcium/lipid-binding and GRAM domain containing protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.3e-15
Identity = 110/540 (20.37%), Postives = 224/540 (41.48%), Query Frame = 0

Query: 50   TGVGYLMLIVLWLMVLEMIPP----GHLRDLQSN-SAYLIKLELLAAKNLIAANLNGTSD 109
            +G+  L+L  +++M +  +      G  + +++N   +++ + L+   NL +       D
Sbjct: 502  SGILVLLLERVYIMTVHFVQARLHRGRDQGVKANGKGWILTIALIKGTNLASVEATELFD 561

Query: 110  PYAIITCGTEKRFSSMIPGSRNPMWGEEFNF-SVDELPVQIHATIYDWDIVW-KSAVLGS 169
            PY + TC  + R SS+   +++P W E   F +++E P  +   ++D+D  + + A LG 
Sbjct: 562  PYVVFTCNGKTRTSSVKLQAQDPQWNEVIEFDAMEEPPSVLDVEVFDFDGPFDQGASLGH 621

Query: 170  VTV-----TVENEGHTGAVWYTLDSTSGQVCLHIKTIKLPVNAGSTVNGYAG---ANARR 229
              +     T +             + + Q  L ++      N   T+  Y         +
Sbjct: 622  AEINFLKHTADELADLSVALVGNHAQASQSKLQLRIFLENKNGVETMKDYLSKVEKEVGK 681

Query: 230  RISLDKPELTVVHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHS 289
            ++++  P      QK    Q +F L  +E +   ++C L+R     G++++S+  + F+S
Sbjct: 682  KLNIRSP------QKNSAFQKLFGLPHEEFLLKEYTCYLKRKLPVQGKLFLSARIVAFYS 741

Query: 290  NIFSKQMKVVIPLGDIDEIRRTQHAFI---NPAVTIILRMGAG---GHGVPPLGSPDGRV 349
            N+F  + K      DID+I+     F    +P + IIL+   G    HG       +GR+
Sbjct: 742  NVFGHKTKFYFLWEDIDDIQVLPPTFASLGSPLLLIILKKNRGLDAKHGAKS-QDDEGRL 801

Query: 350  RYKFASFWNRNHVVRALQR-------SVNNFREMLEAEKKEKAESALRAHSSSVRVSESK 409
             + F SF + +   R +         SV++  +++E E ++ A+  L   + +V VS+  
Sbjct: 802  SFYFQSFVSFDATSRTIMALWKTRTLSVDHRAQIVE-EDQDVADPFLLPEAVTV-VSD-- 861

Query: 410  EKIPADDLPKSKNTQTQAFLKEEVLTSIHNGVFPCSPERFFSTLLSDGSGYTSAFVARRK 469
                AD L  SK               ++    PC  E      +  G       + +  
Sbjct: 862  ----ADALMMSK---------------VYTCDLPCDVELVMK--IFGGGELERKIMEKSG 921

Query: 470  DSNLVMGQWHAADE--YEGQVRELTYRSLCHSPMCPPDTAMTEYQHVVLSEDKKKLVFET 529
              +     W +     YE   R L+Y+   +  +      +T  Q    + + +  +   
Sbjct: 922  CLSYASTTWESKKPGVYE---RRLSYKYNHYVSVF--GGGVTCAQQKSPAPNDEGWILNE 981

Query: 530  VQNAHDVPFGANFELHCRWSLEKNAED--SSSVDIKAGVHFKKWCLMQSKIKAGAMLEYK 558
            +   HDVPFG +F +H R+ ++K   D  +S  ++   + + K    + +I    M +++
Sbjct: 982  IVALHDVPFGDHFRVHIRYEVKKAGVDCKTSKCEVYLKIRWLKTIKFEQRISKSIMEKFR 1004

BLAST of IVF0004180 vs. TAIR 10
Match: AT1G02120.1 (GRAM domain family protein )

HSP 1 Score: 80.9 bits (198), Expect = 3.9e-15
Identity = 93/393 (23.66%), Postives = 147/393 (37.40%), Query Frame = 0

Query: 226 VHQKPGPLQTIFELLPDEIVEHSFSCALERSFLYHGRMYVSSWHICFHSNIFSKQMKVVI 285
           V  K    + +F L  DEI+   F+CA + S L  G MY+   +ICF+SNIF  + K +I
Sbjct: 65  VISKSEEYRQLFRLPADEILVQDFNCACQESILMQGHMYLFIHYICFYSNIFGYETKKII 124

Query: 286 PLGDIDEIRRTQHAFINPAVTIILRMGAGGHGVPPLGSPDGRVRYKFASFWNRNHVVRAL 345
           P  +I  ++R + A I P    IL   AGG             +Y FASF +R+   + +
Sbjct: 125 PFAEISCVKRAKTAGIFPNAIEIL---AGGK------------KYFFASFLSRDEAFKLI 184

Query: 346 Q-------RSVNNFREMLEAEKKEKAESALRAHSSSVRVSE------------------- 405
                    +V +  E+L  E +       RA SS    +E                   
Sbjct: 185 HDGWLEYGSAVKSEGEILVTEPQVSDGVVKRARSSMDLANELDIPVRDETLHLSSSSSLP 244

Query: 406 --SKEKIPADDLPKS---------------KNTQTQAFLKEEVLTSIHNGVFPCSPERFF 465
             S+  +P   + +                K   T A       T +    F    E FF
Sbjct: 245 VISQNGVPPSSVQRHAEPDVDVVAANTFNWKPEDTDAPKLSSDFTKVAEAKFSIPVEEFF 304

Query: 466 STLLSDGS-GYTSAFVARRKDSNLVMGQWHAADEYEGQVRELTYRSLCHSPMCPPDTAMT 525
               SDG+  +  +F     D       W   ++  G  R ++++               
Sbjct: 305 RLFFSDGAVSFVESFHKNCGDKEFRCTSWQPHEKL-GHTRNVSFQHPIKIYFGAKFGGCQ 364

Query: 526 EYQHVVLSEDKKKLVFETVQNAHDVPFGANFELHCRWSLEKNAEDSSS---VDIKAGVHF 572
           E Q   +  +   LV ET Q   DVP+   F +   W L+++  DS     +D+   V F
Sbjct: 365 ESQKFRMYRN-SHLVIETSQEISDVPYADYFTVEGVWDLKRDCRDSVEGCILDVYVNVAF 424

BLAST of IVF0004180 vs. TAIR 10
Match: AT1G05500.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 56.6 bits (135), Expect = 7.8e-08
Identity = 43/156 (27.56%), Postives = 74/156 (47.44%), Query Frame = 0

Query: 64  VLEMIP--PGHLRDLQSNSAYLIKLELLAAKNLIAANLNGTSDPYA---IITCGTEKRFS 123
           V ++IP  PG   DL+     +++++L+ AKNL   +L G SDP+A   I     + + S
Sbjct: 242 VRKVIPIIPGDYSDLELKPVGMLEVKLVQAKNLTNKDLVGKSDPFAKMFIRPLREKTKRS 301

Query: 124 SMIPGSRNPMWGEEFNFSVDELPVQ-IHATIYDWDIVWKSAVLGSVTVTV--ENEGHTGA 183
             I    NP+W E F F V++   Q +   IYD + V  S ++G   + +     G    
Sbjct: 302 KTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGKVKD 361

Query: 184 VWYTL-------DSTSGQVCLHIKTIKLPVNAGSTV 205
           VW  L         T  +  +H++ + +P  +G+ +
Sbjct: 362 VWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSGNGI 397