IVF0003676 (gene) Melon (IVF77) v1

Overview
NameIVF0003676
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionnicotianamine synthase-like
Locationchr05: 22755157 .. 22756961 (-)
RNA-Seq ExpressionIVF0003676
SyntenyIVF0003676
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTTGAACAACAACAACAAGAACAACAGGAAGCACTTCTGTTGCAAAAAGTTTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCAGCCACCAGCTGCCCCAATTGGCTTTGATGTAGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGAGCTCATCTCATTCAACTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAACTACTTGAAGCTTTCCCTTCTCGAGTTTGATATTCTTCGATCCCATTCTCGTCGTCTCCCCAATAAAGTTGCCTTCGTTGGCTCCGGTCCCCTCCCCCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTAACCGTTAGTTCCTGAATTAGTTACTAACAATATATACTGAGAAAGGTTGTTTTATGGTCATGGTTGAATTTTATTATTGCTAGCTTTGATGCCATGTTGAAAGTACTATTGAAATTATTAGATAAACACATGATGATGACTCATTTTAAAGTTATTTAATAATGAAAGGAGTAGTTCGTGTATCTTCTAAACATTGCGAGGTTGGTTTCTCCATCTTTTTAATACGAGATCGTCAGTATGTCCTGAAAATGATTTTTTTTTACCATTATTCTTATGGGCCATTTTTGTTTTGAATTAAATATTTATTTGAATTTTATTGACAAAGTTACCCTGTTAGAAAAACATGAAGTTCCAATTCAAACTCAATTGATAACATTGAGATGAGTAACTCATGTATATTATGAGATCTCTCCTATCTTTTTATCGTAAGGTTTAGGTTTAATGGTATATTTTGTCAATTGATTTGTGGATATTTCATTTGTGATGACAATATAGAGATTTCGAATATATATACAAACCTTATTTTGCTAGTGTTTAAGGTGGGGTAGTTAATATTTTAAGTTTACTTCTTGTTTGGTTTAAAAAGTTTTTAAACTAACAGTTGCCCACAACTTATTAACATCAATTCTATGACTCCTTGTATCGTAAATTCAAAAAGTTTTTAACTTCTTGCATGATATGCCAAACAATGATCAAACTAAACAACTCCGACTAAACAATTTCACTCTGTGTTCGATAATCGTAAATTGTTTTAATTACTTTCATGTTCACATCTATCTGAATAGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACTATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTTGTTATTGCAAGGAAGAAAGTCGTGTTCGATCAAAGCTCGGTCGAGGACGAGGACGAGGACGAGGAGGAGGAGGAGAAGCCAGGTATTTTAGTCGATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATCATTGAAGAGCAACTTTCTTGA

mRNA sequence

ATGGAATTTGAACAACAACAACAAGAACAACAGGAAGCACTTCTGTTGCAAAAAGTTTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCAGCCACCAGCTGCCCCAATTGGCTTTGATGTAGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGAGCTCATCTCATTCAACTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAACTACTTGAAGCTTTCCCTTCTCGAGTTTGATATTCTTCGATCCCATTCTCGTCGTCTCCCCAATAAAGTTGCCTTCGTTGGCTCCGGTCCCCTCCCCCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACTATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTTGTTATTGCAAGGAAGAAAGTCGTGTTCGATCAAAGCTCGGTCGAGGACGAGGACGAGGACGAGGAGGAGGAGGAGAAGCCAGGTATTTTAGTCGATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATCATTGAAGAGCAACTTTCTTGA

Coding sequence (CDS)

ATGGAATTTGAACAACAACAACAAGAACAACAGGAAGCACTTCTGTTGCAAAAAGTTTGTGCCCTTTACAACCAAATCTCAAGCCTTTCTAGCCTTAAGCCTTGTAAAAATGTCGACACTCTTTTCACTCAACTCGTCCTCACGTGCTCACAGCAGCCACCAGCTGCCCCAATTGGCTTTGATGTAGCTTCTTTGTCTCAGCCACTTCAAGCCATGAGAGCTCATCTCATTCAACTTTGTGCTAAAGCTGAGGCTCTTCTTGAACTTCATTTCTCTTCCCTTTTGGCTTCTTCTTTTCATCATCCTATTTCTAATTTGTCTATCTTCCCTTACTACTCTAACTACTTGAAGCTTTCCCTTCTCGAGTTTGATATTCTTCGATCCCATTCTCGTCGTCTCCCCAATAAAGTTGCCTTCGTTGGCTCCGGTCCCCTCCCCCTCTCCTCCATTGTCTTGGCGTCTATTCATCTTAAGGGTACTATTTTCCACAACTTTGACATTGATCCAACAGCAAACACTATGGCAAGTCAATTGGTTTGCTCTGACCCAGACTTATCACAAAGAATGATATTTCACACAAAAGATGTAATGGAAGTAACAAAGGGATTAAAAGATTATGAAGTAGTGTTCTTAGCAGCATTAGTTGGATTAGAAGAGGAAGAAAAAGGAAGAGTTTTAAAGCATTTGGGGAAACATATGGCAGCTGGTTCATATTTGATGCTAAGAAGTGCTCATGGAGCAAGAGCATTTTTGTACCCTGTTGTTGATATTTGTACTGTTGAAGCTTCTGGATTTGAGATTTTGTCTGTGTTCCATCCCACCGATGAAGTTATTAACTCTGTTGTTATTGCAAGGAAGAAAGTCGTGTTCGATCAAAGCTCGGTCGAGGACGAGGACGAGGACGAGGAGGAGGAGGAGAAGCCAGGTATTTTAGTCGATACTTCTTCTATTGTGTTGGCTGAGAAATGCTCTGGGTTTAATGGCTTTACTCCCATCATTGAAGAGCAACTTTCTTGA

Protein sequence

MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDEDEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS
Homology
BLAST of IVF0003676 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 4.7e-87
Identity = 177/331 (53.47%), Postives = 243/331 (73.41%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC    P      DV  +S+ +Q
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNP----NIDVTKMSENIQ 64

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH- 130
            MR++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + 
Sbjct: 65  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 124

Query: 131 SRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 190
           +  +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM
Sbjct: 125 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 184

Query: 191 IFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 250
            FHT D+M+VT+ LK ++VVFLAALVG++++EK +V++HL KHM+ G+ LMLRSAHG RA
Sbjct: 185 FFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRA 244

Query: 251 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDEDEEEEEKPG 310
           FLYP+V+ C +E  GFE+LSV+HPTDEVINS+VI+R K+  D + V  +  D+  +    
Sbjct: 245 FLYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVVHDHIDQASD---- 304

Query: 311 ILVDTSSI--VLAEKCSGFNGFTPIIEEQLS 339
           +  + S I  ++ +K S    F    EEQL+
Sbjct: 305 LACNCSKIHVIMNKKKSIIEEFAGANEEQLT 324

BLAST of IVF0003676 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 3.4e-85
Identity = 165/272 (60.66%), Postives = 213/272 (78.31%), Query Frame = 0

Query: 15  LLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQAMRA 74
           +++KVC LY QIS L +L P K+V+ LFT LV TC    P  PI  DV+ L Q +Q +R+
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCM---PPNPI--DVSKLCQKIQEIRS 68

Query: 75  HLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRRLP 134
           HLI+LC +AE LLE HFS +L SS+ +P+ +L IFPY+ NY+KLSLLE++IL  ++  +P
Sbjct: 69  HLIKLCGQAEGLLESHFSKIL-SSYENPLQHLHIFPYFDNYIKLSLLEYNILTKNTTNIP 128

Query: 135 NKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFHTK 194
            K+AF+GSGPLPL+S+VLA+ HLK T FHN+DID  AN MAS LV +DPD+S RM FHT 
Sbjct: 129 KKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFHTA 188

Query: 195 DVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLYPV 254
           DVM+VT  LKDY+VVFLAALVG+++E+K +V+ HL K+M+ G+ LMLRSAHGARAFLYPV
Sbjct: 189 DVMDVTCALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFLYPV 248

Query: 255 VDICTVEASGFEILSVFHPTDEVINSVVIARK 287
           +D    +  GFE+LSV+HPTDEVINSV+IARK
Sbjct: 249 LD--PRDLRGFEVLSVYHPTDEVINSVIIARK 272

BLAST of IVF0003676 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 3.7e-84
Identity = 164/286 (57.34%), Postives = 215/286 (75.17%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q+  L+Q +C LY +IS L SLKP ++V+ LF QLV TC    P      DV  +   +Q
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNP----NIDVTKMCDRVQ 63

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH- 130
            +R +LI++C  AE  LE HFSS+L S   +P+ +L+IFPYY+NYLKL  LEFD+L  + 
Sbjct: 64  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 123

Query: 131 SRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 190
           +  +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM
Sbjct: 124 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 183

Query: 191 IFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 250
            FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RA
Sbjct: 184 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 243

Query: 251 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSV 296
           FLYP+V+ C ++  GFE+LS++HPTD+VINSVVI++K  V    +V
Sbjct: 244 FLYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

BLAST of IVF0003676 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 161/278 (57.91%), Postives = 207/278 (74.46%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC           DV ++ + ++
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL----PTDTNIDVTNMCEEVK 63

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLAS--SFHHPISNLSIFPYYSNYLKLSLLEFDILRS 130
            MRA+LI+LC +AE  LE HFS++L S     +P+ +L IFPYYSNYLKL  LEFD+L  
Sbjct: 64  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 123

Query: 131 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQR 190
           HS  +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+R
Sbjct: 124 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 183

Query: 191 MIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGAR 250
           MIFHT DV+  T+GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ LMLRSAH  R
Sbjct: 184 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 243

Query: 251 AFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 287
           AFLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 244 AFLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of IVF0003676 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 310.8 bits (795), Expect = 1.8e-83
Identity = 158/274 (57.66%), Postives = 206/274 (75.18%), Query Frame = 0

Query: 14  LLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASL-SQPLQAM 73
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC           DV  +  + ++ M
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL----PTDTNIDVTEIHDEKVKDM 66

Query: 74  RAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRR 133
           R+HLI+LC +AE  LE HFS++L S   +P+++L IFPYY+NYLKL  LEFD+L  H+  
Sbjct: 67  RSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTH 126

Query: 134 LPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 193
           +P KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFH
Sbjct: 127 VPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFH 186

Query: 194 TKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 253
           T DV+   +GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ +MLRSAHG RAFLY
Sbjct: 187 TTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLY 246

Query: 254 PVVDICTVEASGFEILSVFHPTDEVINSVVIARK 287
           P+VD C  +  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 247 PIVDSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of IVF0003676 vs. ExPASy TrEMBL
Match: A0A5D3BBH0 (Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G001110 PE=3 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 339
           DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

BLAST of IVF0003676 vs. ExPASy TrEMBL
Match: A0A0A0LGI4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 9.0e-166
Identity = 313/338 (92.60%), Postives = 324/338 (95.86%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQ    QQEALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQ----QQEALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPP-PPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FDQSS    DE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSS----DE 300

Query: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 339
           DE+E+EK GILV+TSSIVLAEKCSGFNGFTP+IEE LS
Sbjct: 301 DEDEDEKQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

BLAST of IVF0003676 vs. ExPASy TrEMBL
Match: A0A1S3BXI8 (probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 4.9e-156
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 292
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

BLAST of IVF0003676 vs. ExPASy TrEMBL
Match: A0A1S3BWE4 (probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494174 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.6e-154
Identity = 291/293 (99.32%), Postives = 291/293 (99.32%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 292
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

BLAST of IVF0003676 vs. ExPASy TrEMBL
Match: A0A6J1G3L4 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 4.1e-126
Identity = 251/326 (76.99%), Postives = 278/326 (85.28%), Query Frame = 0

Query: 12  EALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQA 71
           E  LL++VCALY+QISSL+SLKP KNVDTLFTQLVLTCS   P AP G D+ASL QPLQA
Sbjct: 2   EGPLLREVCALYDQISSLASLKPSKNVDTLFTQLVLTCS---PPAP-GLDIASLPQPLQA 61

Query: 72  MRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSR 131
           MRAHLI+LCA AEALLE+HFSS+LA SF +PI NLSIFPYYSNYLKLSLLEFDIL SH R
Sbjct: 62  MRAHLIRLCATAEALLEVHFSSILA-SFENPIHNLSIFPYYSNYLKLSLLEFDILTSHCR 121

Query: 132 RLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIF 191
           R+P+ VAFVGSGPLPLSSIV+AS HLKGTIFHNFDIDP AN+MA QLVCSD DLS+RM+F
Sbjct: 122 RVPSSVAFVGSGPLPLSSIVMASTHLKGTIFHNFDIDPVANSMAGQLVCSDSDLSKRMVF 181

Query: 192 HTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFL 251
           HT DVMEVT+   +YEVVFLAALVGLEEEEKGRVL+HLGKHMA G+YLMLRSAHGARAFL
Sbjct: 182 HTTDVMEVTEAFGEYEVVFLAALVGLEEEEKGRVLRHLGKHMAPGAYLMLRSAHGARAFL 241

Query: 252 YPVVDICTVEASGFEILSVFHPTDEVINSVVIARK--KVVFDQSSVEDEDEDEEEEEKPG 311
           YPVVD+  VEASGFEILSVFHPTDEVINSVVIARK   +V D  +  D D D   +EK  
Sbjct: 242 YPVVDVRDVEASGFEILSVFHPTDEVINSVVIARKVSNLVPDNDNYNDNDTD---DEKRA 301

Query: 312 ILVDTSSIVLAEKCSGFNGFTPIIEE 336
           +LV+T  I+LAEKCSGF  F P+IEE
Sbjct: 302 VLVNT-PIILAEKCSGFKDFGPMIEE 318

BLAST of IVF0003676 vs. NCBI nr
Match: KAA0048069.1 (putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ96467.1 putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 648 bits (1672), Expect = 3.66e-234
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300

Query: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338
           DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS
Sbjct: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338

BLAST of IVF0003676 vs. NCBI nr
Match: XP_004144750.1 (probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical protein Csa_021149 [Cucumis sativus])

HSP 1 Score: 592 bits (1526), Expect = 4.66e-212
Identity = 314/338 (92.90%), Postives = 323/338 (95.56%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQ    EALLL+KVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQ PP  PIGF
Sbjct: 1   MEFEQQQ----EALLLEKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQHPPP-PIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPL+AMRAHLIQLCA+AEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DIASLSQPLRAMRAHLIQLCAQAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRR+P+KVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRIPDKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGL EEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLGEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKV+FDQSS EDEDE
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVMFDQSSDEDEDE 300

Query: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338
           DE    K GILV+TSSIVLAEKCSGFNGFTP+IEE LS
Sbjct: 301 DE----KQGILVNTSSIVLAEKCSGFNGFTPMIEEPLS 329

BLAST of IVF0003676 vs. NCBI nr
Match: XP_008453482.1 (PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo])

HSP 1 Score: 561 bits (1447), Expect = 3.50e-200
Identity = 291/291 (100.00%), Postives = 291/291 (100.00%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM
Sbjct: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291
           LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291

BLAST of IVF0003676 vs. NCBI nr
Match: XP_008453473.1 (PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo])

HSP 1 Score: 556 bits (1434), Expect = 3.60e-198
Identity = 291/293 (99.32%), Postives = 291/293 (99.32%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF
Sbjct: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL
Sbjct: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKG--TIFHNFDIDPTANTMASQL 180
           LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKG  TIFHNFDIDPTANTMASQL
Sbjct: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGNRTIFHNFDIDPTANTMASQL 180

Query: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240
           VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY
Sbjct: 181 VCSDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSY 240

Query: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 291
           LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD
Sbjct: 241 LMLRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFD 293

BLAST of IVF0003676 vs. NCBI nr
Match: XP_038890564.1 (probable nicotianamine synthase 4 [Benincasa hispida])

HSP 1 Score: 548 bits (1411), Expect = 1.48e-194
Identity = 287/338 (84.91%), Postives = 309/338 (91.42%), Query Frame = 0

Query: 1   MEFEQQQQEQQEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGF 60
           MEFE       EA LLQK+ ALY+QISSL+SL P KNVDTLFTQLVLTCS   P AP GF
Sbjct: 1   MEFE-------EAPLLQKISALYHQISSLTSLNPSKNVDTLFTQLVLTCS---PPAPAGF 60

Query: 61  DVASLSQPLQAMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSL 120
           D+ASLSQPLQAMRAHLI+LCAKAEALLELHFSS+LASSF +PISNLSIFPYYSNYLKLSL
Sbjct: 61  DIASLSQPLQAMRAHLIRLCAKAEALLELHFSSILASSFRNPISNLSIFPYYSNYLKLSL 120

Query: 121 LEFDILRSHSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVC 180
           LEFDILRSH RR+P  +AF+GSGPLPLSSIV+ASIHLKGTIFHNFD+DPTAN+MASQLVC
Sbjct: 121 LEFDILRSHCRRVPASIAFLGSGPLPLSSIVMASIHLKGTIFHNFDLDPTANSMASQLVC 180

Query: 181 SDPDLSQRMIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLM 240
           SDPDLSQRMIFHT DVM VT GLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAG+YLM
Sbjct: 181 SDPDLSQRMIFHTTDVMNVTNGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGAYLM 240

Query: 241 LRSAHGARAFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDE 300
           LRSAHGARAFLYPVVDIC VEASGFEILSVFHPTDEVINSVVIARKK +FD++SVEDED+
Sbjct: 241 LRSAHGARAFLYPVVDICDVEASGFEILSVFHPTDEVINSVVIARKKALFDRNSVEDEDD 300

Query: 301 DEEEEEKPGILVDTSSIVLAEKCSGFNGFTPIIEEQLS 338
           D+   EK GILVDTSSIVLAEKCSGFNGF+P++EEQLS
Sbjct: 301 DDNAAEKRGILVDTSSIVLAEKCSGFNGFSPMVEEQLS 328

BLAST of IVF0003676 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 322.8 bits (826), Expect = 3.3e-88
Identity = 177/331 (53.47%), Postives = 243/331 (73.41%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q+  L+ K+C LY +IS L +LKPC++VDTLF QLV TC    P      DV  +S+ +Q
Sbjct: 5   QDDQLVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNP----NIDVTKMSENIQ 64

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH- 130
            MR++LI++C +AE  LE HFSS+L S   +P+ +L++FPYY+NYLKLS LEFD+L  + 
Sbjct: 65  EMRSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNL 124

Query: 131 SRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 190
           +  +P  VAF+GSGPLPL+S+VLAS HLK +IFHNFDIDP+AN +A++LV SDPDLSQRM
Sbjct: 125 NGFVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRM 184

Query: 191 IFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 250
            FHT D+M+VT+ LK ++VVFLAALVG++++EK +V++HL KHM+ G+ LMLRSAHG RA
Sbjct: 185 FFHTVDIMDVTESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRA 244

Query: 251 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSVEDEDEDEEEEEKPG 310
           FLYP+V+ C +E  GFE+LSV+HPTDEVINS+VI+R K+  D + V  +  D+  +    
Sbjct: 245 FLYPIVEPCDLE--GFEVLSVYHPTDEVINSIVISR-KLGEDANGVVHDHIDQASD---- 304

Query: 311 ILVDTSSI--VLAEKCSGFNGFTPIIEEQLS 339
           +  + S I  ++ +K S    F    EEQL+
Sbjct: 305 LACNCSKIHVIMNKKKSIIEEFAGANEEQLT 324

BLAST of IVF0003676 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 313.2 bits (801), Expect = 2.6e-85
Identity = 164/286 (57.34%), Postives = 215/286 (75.17%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q+  L+Q +C LY +IS L SLKP ++V+ LF QLV TC    P      DV  +   +Q
Sbjct: 4   QDEQLVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNP----NIDVTKMCDRVQ 63

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSH- 130
            +R +LI++C  AE  LE HFSS+L S   +P+ +L+IFPYY+NYLKL  LEFD+L  + 
Sbjct: 64  EIRLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNL 123

Query: 131 SRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRM 190
           +  +P  VAF+GSGPLPL+SIVLAS HLK TIFHNFDIDP+AN++AS LV SDPD+SQRM
Sbjct: 124 NGFVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRM 183

Query: 191 IFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARA 250
            FHT D+M+VT+ LK ++VVFLAALVG+ +EEK +V++HL KHMA G+ LMLRSAHG RA
Sbjct: 184 FFHTVDIMDVTESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRA 243

Query: 251 FLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARKKVVFDQSSV 296
           FLYP+V+ C ++  GFE+LS++HPTD+VINSVVI++K  V    +V
Sbjct: 244 FLYPIVEPCDLQ--GFEVLSIYHPTDDVINSVVISKKHPVVSIGNV 283

BLAST of IVF0003676 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-84
Identity = 161/278 (57.91%), Postives = 207/278 (74.46%), Query Frame = 0

Query: 11  QEALLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASLSQPLQ 70
           Q  L+++++  LY+QIS L SLKP KNVDTLF QLV TC           DV ++ + ++
Sbjct: 4   QNNLVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL----PTDTNIDVTNMCEEVK 63

Query: 71  AMRAHLIQLCAKAEALLELHFSSLLAS--SFHHPISNLSIFPYYSNYLKLSLLEFDILRS 130
            MRA+LI+LC +AE  LE HFS++L S     +P+ +L IFPYYSNYLKL  LEFD+L  
Sbjct: 64  DMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKLEFDLLSQ 123

Query: 131 HSRRLPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQR 190
           HS  +P K+AFVGSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  DPDLS+R
Sbjct: 124 HSSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDPDLSKR 183

Query: 191 MIFHTKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGAR 250
           MIFHT DV+  T+GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ LMLRSAH  R
Sbjct: 184 MIFHTTDVLNATEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVLMLRSAHALR 243

Query: 251 AFLYPVVDICTVEASGFEILSVFHPTDEVINSVVIARK 287
           AFLYP+VD  + +  GF++L+++HPTD+V+NSVVIARK
Sbjct: 244 AFLYPIVD--SSDLKGFQLLTIYHPTDDVVNSVVIARK 275

BLAST of IVF0003676 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 310.8 bits (795), Expect = 1.3e-84
Identity = 158/274 (57.66%), Postives = 206/274 (75.18%), Query Frame = 0

Query: 14  LLLQKVCALYNQISSLSSLKPCKNVDTLFTQLVLTCSQQPPAAPIGFDVASL-SQPLQAM 73
           L+++++  LYNQIS+L SLKP KNVDTLF QLV TC           DV  +  + ++ M
Sbjct: 7   LVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL----PTDTNIDVTEIHDEKVKDM 66

Query: 74  RAHLIQLCAKAEALLELHFSSLLASSFHHPISNLSIFPYYSNYLKLSLLEFDILRSHSRR 133
           R+HLI+LC +AE  LE HFS++L S   +P+++L IFPYY+NYLKL  LEFD+L  H+  
Sbjct: 67  RSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLEFDLLSQHTTH 126

Query: 134 LPNKVAFVGSGPLPLSSIVLASIHLKGTIFHNFDIDPTANTMASQLVCSDPDLSQRMIFH 193
           +P KVAF+GSGP+PL+SIVLA  HL  T FHNFDID  ANT+AS LV  D DLS+RMIFH
Sbjct: 127 VPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSRDSDLSKRMIFH 186

Query: 194 TKDVMEVTKGLKDYEVVFLAALVGLEEEEKGRVLKHLGKHMAAGSYLMLRSAHGARAFLY 253
           T DV+   +GL  Y+VVFLAALVG+++E K + ++HL KHMA G+ +MLRSAHG RAFLY
Sbjct: 187 TTDVLNAKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVMLRSAHGLRAFLY 246

Query: 254 PVVDICTVEASGFEILSVFHPTDEVINSVVIARK 287
           P+VD C  +  GFE+L+++HP+D+V+NSVVIARK
Sbjct: 247 PIVDSC--DLKGFEVLTIYHPSDDVVNSVVIARK 274

BLAST of IVF0003676 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-07
Identity = 29/56 (51.79%), Postives = 39/56 (69.64%), Query Frame = 0

Query: 233 MAAGSYLMLRSAHGARAFLYPVVDICTVEASGFEILSVFHP--TDEVINSVVIARK 287
           M  G+ LMLRSA G R+FLY  VD C  +  GFE+L ++HP  +D  +NSV++ARK
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPC--DLKGFEVLEIYHPSMSDGFVNSVMVARK 54

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C7X54.7e-8753.47Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S... [more]
Q9XGI73.4e-8560.66Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1[more]
O804833.7e-8457.34Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1[more]
Q9FF791.8e-8357.91Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1[more]
Q9FKT91.8e-8357.66Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BBH01.8e-182100.00Putative nicotianamine synthase 4 isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0LGI49.0e-16692.60Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034530 PE=3 SV=1[more]
A0A1S3BXI84.9e-156100.00probable nicotianamine synthase 4 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A1S3BWE41.6e-15499.32probable nicotianamine synthase 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349... [more]
A0A6J1G3L44.1e-12676.99nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111450477 PE=3 S... [more]
Match NameE-valueIdentityDescription
KAA0048069.13.66e-234100.00putative nicotianamine synthase 4 isoform X2 [Cucumis melo var. makuwa] >TYJ9646... [more]
XP_004144750.14.66e-21292.90probable nicotianamine synthase 4 [Cucumis sativus] >KGN61000.1 hypothetical pro... [more]
XP_008453482.13.50e-200100.00PREDICTED: probable nicotianamine synthase 4 isoform X2 [Cucumis melo][more]
XP_008453473.13.60e-19899.32PREDICTED: probable nicotianamine synthase 4 isoform X1 [Cucumis melo][more]
XP_038890564.11.48e-19484.91probable nicotianamine synthase 4 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G56430.13.3e-8853.47nicotianamine synthase 4 [more]
AT1G09240.12.6e-8557.34nicotianamine synthase 3 [more]
AT5G04950.11.3e-8457.91nicotianamine synthase 1 [more]
AT5G56080.11.3e-8457.66nicotianamine synthase 2 [more]
AT4G26483.11.7e-0751.79nicotianamine synthases [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 16..288
e-value: 6.6E-60
score: 204.5
IPR004298Nicotianamine synthasePFAMPF03059NAScoord: 9..288
e-value: 5.2E-113
score: 377.0
IPR004298Nicotianamine synthasePANTHERPTHR32266NICOTIANAMINE SYNTHASE 3coord: 8..288
IPR004298Nicotianamine synthasePROSITEPS51142NAScoord: 7..291
score: 53.211639

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0003676.2IVF0003676.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030418 nicotianamine biosynthetic process
molecular_function GO:0030410 nicotianamine synthase activity