IVF0003358 (gene) Melon (IVF77) v1

Overview
NameIVF0003358
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon 17.6
Locationchr10: 18890538 .. 18890822 (-)
RNA-Seq ExpressionIVF0003358
SyntenyIVF0003358
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTACGACGAAAGTGATGTAGCGGTAGGAGCTATGCTAGGTCAAAAGGAGGATAAAATGATTCATCTTAGATCCTACACAAGCAAGACTCTGAATGAAGGTCAAGAGAACTACACAACCACAAAAAAGGAATTGCTCGCGGTAGTATTCGAGATAGAGAAATATAGGAGCTACATTATTGGTTCCAAAGTCACGATACACTCTAAATCATTCTGTGATCAGATATTTGATGGCAAAGGACACCAAGCTGCGAATCAAATGGATATTATTGCTCCAAGAATTTGA

mRNA sequence

ATGTACGACGAAAGTGATGTAGCGGTAGGAGCTATGCTAGGTCAAAAGGAGGATAAAATGATTCATCTTAGATCCTACACAAGCAAGACTCTGAATGAAGGTCAAGAGAACTACACAACCACAAAAAAGGAATTGCTCGCGGTAGTATTCGAGATAGAGAAATATAGGAGCTACATTATTGGTTCCAAAGTCACGATACACTCTAAATCATTCTGTGATCAGATATTTGATGGCAAAGGACACCAAGCTGCGAATCAAATGGATATTATTGCTCCAAGAATTTGA

Coding sequence (CDS)

ATGTACGACGAAAGTGATGTAGCGGTAGGAGCTATGCTAGGTCAAAAGGAGGATAAAATGATTCATCTTAGATCCTACACAAGCAAGACTCTGAATGAAGGTCAAGAGAACTACACAACCACAAAAAAGGAATTGCTCGCGGTAGTATTCGAGATAGAGAAATATAGGAGCTACATTATTGGTTCCAAAGTCACGATACACTCTAAATCATTCTGTGATCAGATATTTGATGGCAAAGGACACCAAGCTGCGAATCAAATGGATATTATTGCTCCAAGAATTTGA

Protein sequence

MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYIIGSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI
Homology
BLAST of IVF0003358 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.9e-07
Identity = 31/66 (46.97%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 3   DESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYIIGS 62
           D SDVA+GA+L Q      H  SY S+TLNE + NY+T +KELLA+V+  + +R Y++G 
Sbjct: 514 DASDVALGAVLSQDG----HPLSYISRTLNEHEINYSTIEKELLAIVWATKTFRHYLLGR 573

Query: 63  KVTIHS 69
              I S
Sbjct: 574 HFEISS 575

BLAST of IVF0003358 vs. ExPASy Swiss-Prot
Match: Q8I7P9 (Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.6e-05
Identity = 22/60 (36.67%), Postives = 41/60 (68.33%), Query Frame = 0

Query: 3   DESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYIIGS 62
           D S+ A+GA+L Q +       +Y S++LN+ +ENY T +KE+LA+++ ++  R+Y+ G+
Sbjct: 440 DASNWAIGAVLSQDDQGRDRPIAYISRSLNKTEENYATIEKEMLAIIWSLDNLRAYLYGA 499

BLAST of IVF0003358 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 6.2e-05
Identity = 26/66 (39.39%), Postives = 43/66 (65.15%), Query Frame = 0

Query: 3   DESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYIIGS 62
           D S++A+GA+L Q      H  S+ S+TLN+ + NY+  +KELLA+V+  + +R Y++G 
Sbjct: 513 DASNLALGAVLSQNG----HPISFISRTLNDHELNYSAIEKELLAIVWATKTFRHYLLGR 572

Query: 63  KVTIHS 69
           +  I S
Sbjct: 573 QFLIAS 574

BLAST of IVF0003358 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 8.9e-04
Identity = 23/58 (39.66%), Postives = 38/58 (65.52%), Query Frame = 0

Query: 3   DESDVAVGAMLGQK-EDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYI 60
           D SDVAVGA+L QK +D   +   Y S  +++ Q NY+ + KE+LA++  ++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

BLAST of IVF0003358 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 44.3 bits (103), Expect = 8.9e-04
Identity = 23/58 (39.66%), Postives = 38/58 (65.52%), Query Frame = 0

Query: 3   DESDVAVGAMLGQK-EDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYI 60
           D SDVAVGA+L QK +D   +   Y S  +++ Q NY+ + KE+LA++  ++ +R Y+
Sbjct: 713 DASDVAVGAVLSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLAIIKSLKHWRHYL 770

BLAST of IVF0003358 vs. ExPASy TrEMBL
Match: A0A5A7US86 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G002330 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.9e-45
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 0

Query: 1  MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
          MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII
Sbjct: 1  MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60

Query: 61 GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI 95
          GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI
Sbjct: 61 GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI 94

BLAST of IVF0003358 vs. ExPASy TrEMBL
Match: A0A5A7V8R9 (RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G003510 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 8.7e-18
Identity = 52/71 (73.24%), Postives = 57/71 (80.28%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV +GAMLGQK DK+I+     SKTLNE QENYTTT+KELLAVVF IEKY SYI+
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSKSF 72
           GSKVTIHS  F
Sbjct: 90  GSKVTIHSDHF 100

BLAST of IVF0003358 vs. ExPASy TrEMBL
Match: A0A5A7TFL9 (Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35G00930 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.3e-17
Identity = 48/66 (72.73%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV VGAM+GQ++DK+IH  +YTSKTLNE QENYTTT+KELL VVF IEKYRSYI+
Sbjct: 99  MCDVSDVEVGAMVGQRKDKVIHPINYTSKTLNEVQENYTTTEKELLMVVFAIEKYRSYIV 158

Query: 61  GSKVTI 67
           G+KV +
Sbjct: 159 GTKVMV 164

BLAST of IVF0003358 vs. ExPASy TrEMBL
Match: A0A5D3DZR6 (Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2406G00210 PE=4 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 7.3e-17
Identity = 48/66 (72.73%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV VGAM+GQ++DK+IH  +YTSKTLNE QENYTTT+KELL VVF IEKYRSYI+
Sbjct: 99  MCDVSDVEVGAMVGQRKDKVIHPINYTSKTLNEVQENYTTTEKELLMVVFAIEKYRSYIV 158

Query: 61  GSKVTI 67
           G+KV +
Sbjct: 159 GTKVMV 164

BLAST of IVF0003358 vs. ExPASy TrEMBL
Match: A0A5D3C0M6 (Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001400 PE=4 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 1.2e-16
Identity = 46/67 (68.66%), Postives = 55/67 (82.09%), Query Frame = 0

Query: 1  MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
          MYD SDV V AMLGQK+DK+IHL  Y SKTLNE +ENY T +KELL VVF +EKY+SYI+
Sbjct: 1  MYDTSDVVVWAMLGQKKDKVIHLIYYASKTLNEAEENYMTVEKELLVVVFVVEKYKSYIV 60

Query: 61 GSKVTIH 68
          G +VT+H
Sbjct: 61 GYEVTMH 67

BLAST of IVF0003358 vs. NCBI nr
Match: KAA0056421.1 (Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa] >TYK29099.1 Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuwa])

HSP 1 Score: 189 bits (481), Expect = 1.22e-60
Identity = 94/94 (100.00%), Postives = 94/94 (100.00%), Query Frame = 0

Query: 1  MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
          MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII
Sbjct: 1  MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60

Query: 61 GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI 94
          GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI
Sbjct: 61 GSKVTIHSKSFCDQIFDGKGHQAANQMDIIAPRI 94

BLAST of IVF0003358 vs. NCBI nr
Match: KAA0062295.1 (hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK26691.1 hypothetical protein E5676_scaffold313G003510 [Cucumis melo var. makuwa])

HSP 1 Score: 100 bits (250), Expect = 3.87e-25
Identity = 52/71 (73.24%), Postives = 57/71 (80.28%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV +GAMLGQK DK+I+     SKTLNE QENYTTT+KELLAVVF IEKY SYI+
Sbjct: 30  MCDASDVVIGAMLGQKNDKVIYPIYNMSKTLNEAQENYTTTEKELLAVVFAIEKYVSYIV 89

Query: 61  GSKVTIHSKSF 71
           GSKVTIHS  F
Sbjct: 90  GSKVTIHSDHF 100

BLAST of IVF0003358 vs. NCBI nr
Match: KAA0066344.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK00934.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 94.4 bits (233), Expect = 3.23e-22
Identity = 44/69 (63.77%), Postives = 58/69 (84.06%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D S+VAV AMLGQK+DK+IHL  Y +KTLN+ +EN+TT +KELLA++  IEK+RSYI+
Sbjct: 75  MCDSSNVAVEAMLGQKKDKVIHLIYYVNKTLNKARENFTTIEKELLAIILTIEKFRSYIV 134

Query: 61  GSKVTIHSK 69
           G KVT+H+K
Sbjct: 135 GFKVTVHNK 143

BLAST of IVF0003358 vs. NCBI nr
Match: KAA0040451.1 (Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 97.8 bits (242), Expect = 7.01e-22
Identity = 48/66 (72.73%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV VGAM+GQ++DK+IH  +YTSKTLNE QENYTTT+KELL VVF IEKYRSYI+
Sbjct: 99  MCDVSDVEVGAMVGQRKDKVIHPINYTSKTLNEVQENYTTTEKELLMVVFAIEKYRSYIV 158

Query: 61  GSKVTI 66
           G+KV +
Sbjct: 159 GTKVMV 164

BLAST of IVF0003358 vs. NCBI nr
Match: TYK28899.1 (Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 97.8 bits (242), Expect = 7.01e-22
Identity = 48/66 (72.73%), Postives = 57/66 (86.36%), Query Frame = 0

Query: 1   MYDESDVAVGAMLGQKEDKMIHLRSYTSKTLNEGQENYTTTKKELLAVVFEIEKYRSYII 60
           M D SDV VGAM+GQ++DK+IH  +YTSKTLNE QENYTTT+KELL VVF IEKYRSYI+
Sbjct: 99  MCDVSDVEVGAMVGQRKDKVIHPINYTSKTLNEVQENYTTTEKELLMVVFAIEKYRSYIV 158

Query: 61  GSKVTI 66
           G+KV +
Sbjct: 159 GTKVMV 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P043233.9e-0746.97Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
Q8I7P91.6e-0536.67Retrovirus-related Pol polyprotein from transposon opus OS=Drosophila melanogast... [more]
P208256.2e-0539.39Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
Q9UR078.9e-0439.66Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT418.9e-0439.66Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
Match NameE-valueIdentityDescription
A0A5A7US864.9e-45100.00Retrovirus-related Pol polyprotein from transposon 17.6 OS=Cucumis melo var. mak... [more]
A0A5A7V8R98.7e-1873.24RT_RNaseH domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
A0A5A7TFL97.3e-1772.73Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3DZR67.3e-1772.73Transposon Ty3-I Gag-Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3C0M61.2e-1668.66Pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G0014... [more]
Match NameE-valueIdentityDescription
KAA0056421.11.22e-60100.00Retrovirus-related Pol polyprotein from transposon 17.6 [Cucumis melo var. makuw... [more]
KAA0062295.13.87e-2573.24hypothetical protein E6C27_scaffold154G00380 [Cucumis melo var. makuwa] >TYK2669... [more]
KAA0066344.13.23e-2263.77reverse transcriptase [Cucumis melo var. makuwa] >TYK00934.1 reverse transcripta... [more]
KAA0040451.17.01e-2272.73Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa][more]
TYK28899.17.01e-2272.73Transposon Ty3-I Gag-Pol polyprotein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 3..66
e-value: 9.6E-17
score: 60.8
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 2..72

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0003358.1IVF0003358.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
biological_process GO:0006278 RNA-dependent DNA biosynthetic process
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0003964 RNA-directed DNA polymerase activity