IVF0003254 (gene) Melon (IVF77) v1

Overview


Name	IVF0003254
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	RING/U-box superfamily protein
Location	chr01: 30779332 .. 30780012 (+)
RNA-Seq Expression	IVF0003254
Synteny	IVF0003254

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGTTGTGTGAGATTTGTCTAGAGTACAAAGAAGAGGGAAACATGTTCGAAATCAAGACGTGTCTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCCACGAAGATCGGCGATGGATATTCCTCCGTTTCTTGTCTTACTTTGAACTGTTCTTCCATTATAGATTTTCACTCTTGCTGGTAATTATAAGTAAATTTAGGGTTTATAAAAGTTGAGGTTTGTAGAAAATGACGATCGAGGGAATTACTTAAACTTTGCAGGACAATGCTACCGAATGAAACAGCTGAGAAATGGAACAAAGCACTTTGCGAAGTGGTTTATCGTGAAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCGGTGGGAATGATCGTTGAGAGAGACGCGAAAATAAGGGATTGTGAGTGTCCGCTTTGCCATAGATTGTTTTGCGGAACGTGTCGAGTCCCATGGCACGAGGGTGTTACGTGCGAGGATTATAAGAGACTTTATAAATATGAAGATGGTAGAGATGAAATTTTAATGAAAAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCCAATTTTTTGTTGAGAAGGTTTCTGGTTGTTTGCATATCACTTGCAGGTTCATTTCTAACCTCCCCCTCTTCTCTTACTTTTTTTCAAATTTTTAG

mRNA sequence

ATGAAGAAGTTGTGTGAGATTTGTCTAGAGTACAAAGAAGAGGGAAACATGTTCGAAATCAAGACGTGTCTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCCACGAAGATCGGCGATGGATATTCCTCCGTTTCTTGTCTTACTTTGAACTGTTCTTCCATTATAGATTTTCACTCTTGCTGGACAATGCTACCGAATGAAACAGCTGAGAAATGGAACAAAGCACTTTGCGAAGTGGTTTATCGTGAAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCGGTGGGAATGATCGTTGAGAGAGACGCGAAAATAAGGGATTGTGAGTGTCCGCTTTGCCATAGATTGTTTTGCGGAACGTGTCGAGTCCCATGGCACGAGGGTGTTACGTGCGAGGATTATAAGAGACTTTATAAATATGAAGATGGTAGAGATGAAATTTTAATGAAAAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCCAATTTTTTGTTGAGAAGGTTTCTGGTTGTTTGCATATCACTTGCAGGTTCATTTCTAACCTCCCCCTCTTCTCTTACTTTTTTTCAAATTTTTAG

Coding sequence (CDS)

ATGAAGAAGTTGTGTGAGATTTGTCTAGAGTACAAAGAAGAGGGAAACATGTTCGAAATCAAGACGTGTCTACACTCATTTTGCCGTGATTGTGTAATCAAACACGTCTCCACGAAGATCGGCGATGGATATTCCTCCGTTTCTTGTCTTACTTTGAACTGTTCTTCCATTATAGATTTTCACTCTTGCTGGACAATGCTACCGAATGAAACAGCTGAGAAATGGAACAAAGCACTTTGCGAAGTGGTTTATCGTGAAGAAGAGAAAGTGGTTTGCCCATTCAAGGATTGCTCGGTGGGAATGATCGTTGAGAGAGACGCGAAAATAAGGGATTGTGAGTGTCCGCTTTGCCATAGATTGTTTTGCGGAACGTGTCGAGTCCCATGGCACGAGGGTGTTACGTGCGAGGATTATAAGAGACTTTATAAATATGAAGATGGTAGAGATGAAATTTTAATGAAAAAATTAGCTTCTCAAATGAAATGGATGAAATGTCCCAAATGCCAATTTTTTGTTGAGAAGGTTTCTGGTTGTTTGCATATCACTTGCAGGTTCATTTCTAACCTCCCCCTCTTCTCTTACTTTTTTTCAAATTTTTAG

Protein sequence

MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHITCRFISNLPLFSYFFSNF

Homology

[Close]

BLAST of IVF0003254 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.2e-15
Identity = 47/193 (24.35%), Postives = 90/193 (46.63%), Query Frame = 0


Query: 5   CEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCW 64
           C +CL   E+ ++  +  C    C +C+ +++S+++  G + + C    C+  +D  +  
Sbjct: 3   CRVCL---EDRSIKPLPCCKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTIL 62

Query: 65  TMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRD---------CECP 124
             LP++   K+ K   E+   +     CP   C      +R   I +          +CP
Sbjct: 63  YSLPHDDIIKY-KYFLELSRMDSSTKPCP--QCKHFTTFKRKTHIPNPTKSENKLKIQCP 122

Query: 125 LCHRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKW-----MKCPKCQF 184
            C  ++C  C  PWHEGV C +YK        + + L++  A++++       KCP+C+ 
Sbjct: 123 SCQFIWCFRCHAPWHEGVNCREYK--------KGDKLLRHWANEIEHGQRNAQKCPRCKV 181

[Close]

BLAST of IVF0003254 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 8.2e-15
Identity = 50/195 (25.64%), Postives = 88/195 (45.13%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           M  +C +CLE K    +  +  C  + C +C+  ++S+++  G   + C    C   ++ 
Sbjct: 232 MVLMCRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFLEE 291

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCP-------FKDCSVGMIVERDAKIRDCE 120
            +    L +E + K+ K   E+   +     CP       FK         R       +
Sbjct: 292 TTVVYNLTHEDSIKY-KYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYKIQ 351

Query: 121 CPLCHRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKW-----MKCPKC 180
           CP C  ++C  C  PWHEGV C++YK        + + L++  AS+++       KCPKC
Sbjct: 352 CPTCQLIWCFKCHSPWHEGVNCKEYK--------KGDKLLRHWASEIEHGQRNAQKCPKC 411

Query: 181 QFFVEKVSGCLHITC 184
           +  +++  GC H+TC
Sbjct: 412 KIHIQRTEGCDHMTC 414

[Close]

BLAST of IVF0003254 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-14
Identity = 50/195 (25.64%), Postives = 87/195 (44.62%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           M  +C +CLE K    +  +  C  + C +C+  ++S ++  G   + C    C   ++ 
Sbjct: 259 MVLMCRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFLEE 318

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCP-------FKDCSVGMIVERDAKIRDCE 120
            +    L +E + K+ K   E+   +     CP       FK         R       +
Sbjct: 319 TTVVYNLTHEDSIKY-KYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYKIQ 378

Query: 121 CPLCHRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKW-----MKCPKC 180
           CP C  ++C  C  PWHEGV C++YK        + + L++  AS+++       KCPKC
Sbjct: 379 CPTCQFVWCFKCHSPWHEGVNCKEYK--------KGDKLLRHWASEIEHGQRNAQKCPKC 438

Query: 181 QFFVEKVSGCLHITC 184
           +  +++  GC H+TC
Sbjct: 439 KIHIQRTEGCDHMTC 441

[Close]

BLAST of IVF0003254 vs. ExPASy Swiss-Prot
Match: A2VEA3 (E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-13
Identity = 57/187 (30.48%), Postives = 88/187 (47.06%), Query Frame = 0


Query: 5   CEIC-LEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKI---GDGYSSVSCLTLNCSSIIDF 64
           C+IC L Y    + F    C H FC  C  ++++TKI   G G  ++SC    C  ++D 
Sbjct: 184 CQICYLNY--PNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMG-QTISCPAHGCDILVDD 243

Query: 65  HSCWTMLPNETAEKWNKALCEVVYREEEKVV--CPFKDC-SVGMIVERDAKIRDCECPLC 124
           ++   ++ +   +   + L    + E  +++  CP  DC  V  +   DAK   C+   C
Sbjct: 244 NTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCK---C 303

Query: 125 HRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSG 184
            R FC  C   WH+ V C+  K+  K  D  D      +A+  K  +CPKC   +EK  G
Sbjct: 304 GRQFCFNCGENWHDPVKCKWLKKWIKKCDD-DSETSNWIAANTK--ECPKCHVTIEKDGG 361

[Close]

BLAST of IVF0003254 vs. ExPASy Swiss-Prot
Match: Q9Y4X5 (E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 5.9e-13
Identity = 57/187 (30.48%), Postives = 88/187 (47.06%), Query Frame = 0


Query: 5   CEIC-LEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKI---GDGYSSVSCLTLNCSSIIDF 64
           C+IC L Y    + F    C H FC  C  ++++TKI   G G  ++SC    C  ++D 
Sbjct: 186 CQICYLNY--PNSYFTGLECGHKFCMQCWSEYLTTKIMEEGMG-QTISCPAHGCDILVDD 245

Query: 65  HSCWTMLPNETAEKWNKALCEVVYREEEKVV--CPFKDC-SVGMIVERDAKIRDCECPLC 124
           ++   ++ +   +   + L    + E  +++  CP  DC  V  +   DAK   C+   C
Sbjct: 246 NTVMRLITDSKVKLKYQHLITNSFVECNRLLKWCPAPDCHHVVKVQYPDAKPVRCK---C 305

Query: 125 HRLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSG 184
            R FC  C   WH+ V C+  K+  K  D  D      +A+  K  +CPKC   +EK  G
Sbjct: 306 GRQFCFNCGENWHDPVKCKWLKKWIKKCDD-DSETSNWIAANTK--ECPKCHVTIEKDGG 363

[Close]

BLAST of IVF0003254 vs. ExPASy TrEMBL
Match: A0A5A7UU31 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold339G001540 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.4e-110
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF
Sbjct: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120
           HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL
Sbjct: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120

Query: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180
           FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH
Sbjct: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180

Query: 181 ITCR 185
           ITCR
Sbjct: 181 ITCR 184

[Close]

BLAST of IVF0003254 vs. ExPASy TrEMBL
Match: A0A5D3CGK1 (Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G001400 PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.5e-109
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF
Sbjct: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120
           HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL
Sbjct: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120

Query: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180
           FCGTCRVPWHEGVTCEDYKRLYKYE+GRDEILMKKLASQMKWMKCPKCQ FVEKVSGCLH
Sbjct: 121 FCGTCRVPWHEGVTCEDYKRLYKYEEGRDEILMKKLASQMKWMKCPKCQLFVEKVSGCLH 180

Query: 181 ITCR 185
           ITCR
Sbjct: 181 ITCR 184

[Close]

BLAST of IVF0003254 vs. ExPASy TrEMBL
Match: A0A0A0K9X9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.4e-94
Identity = 158/187 (84.49%), Postives = 168/187 (89.84%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           MKKLCEICLE KEEGNMFEI TCLHSFCRDCVIKHVSTKI +GYSSVSCL LNC SIIDF
Sbjct: 1   MKKLCEICLENKEEGNMFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDF 60

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120
           HSCW MLP E  EKWNKALCEV+Y  EEKVVCPFKDCSVG+ +ER A IRDCECPLCHRL
Sbjct: 61  HSCWRMLPKEIGEKWNKALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRL 120

Query: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180
           FC TC VPWH+GVTCE+YKRLYK E+GR+EILMKKLASQMKWMKCPKC FFVEK+ GCLH
Sbjct: 121 FCATCEVPWHDGVTCEEYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLH 180

Query: 181 ITCRFIS 188
           ITCRFI+
Sbjct: 181 ITCRFIN 187

[Close]

BLAST of IVF0003254 vs. ExPASy TrEMBL
Match: A0A1S3CDQ5 (probable E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo OX=3656 GN=LOC103499864 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.8e-65
Identity = 115/119 (96.64%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 125
           MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC
Sbjct: 1   MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 60

Query: 126 RVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHITCR 185
           RVPWHEGVTCEDYKRLYKYE+ RDEILM+KLASQMKWMKCPKCQ FVEKVSGCLHITCR
Sbjct: 61  RVPWHEGVTCEDYKRLYKYEEIRDEILMEKLASQMKWMKCPKCQLFVEKVSGCLHITCR 119

[Close]

BLAST of IVF0003254 vs. ExPASy TrEMBL
Match: A0A6J1CX43 (probable E3 ubiquitin-protein ligase RNF217 OS=Momordica charantia OX=3673 GN=LOC111015371 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 7.1e-62
Identity = 111/189 (58.73%), Postives = 139/189 (73.54%), Query Frame = 0


Query: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93

Query: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMI---VERDAKIRDCECPLCH 121
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153

Query: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYED-GR--DEILMKKLASQMKWMKCPKCQFFVEKV 181
           RLFC  CRVPWH  VTCE+Y+R  K +  GR  D+ L+K +A +M+WM+CP+C+FFVEK 
Sbjct: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213

Query: 182 SGCLHITCR 185
            GCLHITCR
Sbjct: 214 YGCLHITCR 222

[Close]

BLAST of IVF0003254 vs. NCBI nr
Match: KAA0058684.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 406 bits (1044), Expect = 6.00e-143
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF
Sbjct: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120
           HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL
Sbjct: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120

Query: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180
           FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH
Sbjct: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180

Query: 181 ITCR 184
           ITCR
Sbjct: 181 ITCR 184

[Close]

BLAST of IVF0003254 vs. NCBI nr
Match: TYK10490.1 (putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa])

HSP 1 Score: 402 bits (1034), Expect = 2.01e-141
Identity = 182/184 (98.91%), Postives = 183/184 (99.46%), Query Frame = 0


Query: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60
           MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF
Sbjct: 1   MKKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 60

Query: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120
           HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL
Sbjct: 61  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRL 120

Query: 121 FCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLH 180
           FCGTCRVPWHEGVTCEDYKRLYKYE+GRDEILMKKLASQMKWMKCPKCQ FVEKVSGCLH
Sbjct: 121 FCGTCRVPWHEGVTCEDYKRLYKYEEGRDEILMKKLASQMKWMKCPKCQLFVEKVSGCLH 180

Query: 181 ITCR 184
           ITCR
Sbjct: 181 ITCR 184

[Close]

BLAST of IVF0003254 vs. NCBI nr
Match: KAE8646419.1 (hypothetical protein Csa_016113 [Cucumis sativus])

HSP 1 Score: 319 bits (817), Expect = 1.36e-108
Identity = 141/168 (83.93%), Postives = 150/168 (89.29%), Query Frame = 0


Query: 17  MFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCWTMLPNETAEKWN 76
           MFEI TCLHSFCRDCVIKHVSTKI +GYSSVSCL LNC SIIDFHSCW MLP E  EKWN
Sbjct: 1   MFEINTCLHSFCRDCVIKHVSTKIENGYSSVSCLALNCPSIIDFHSCWRMLPKEIGEKWN 60

Query: 77  KALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTCRVPWHEGVTCE 136
           KALCEV+Y  EEKVVCPFKDCSVG+ +ER A IRDCECPLCHRLFC TC VPWH+GVTCE
Sbjct: 61  KALCEVLYSTEEKVVCPFKDCSVGITLERGASIRDCECPLCHRLFCATCEVPWHDGVTCE 120

Query: 137 DYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHITCR 184
           +YKRLYK E+GR+EILMKKLASQMKWMKCPKC FFVEK+ GCLHITCR
Sbjct: 121 EYKRLYKNEEGRNEILMKKLASQMKWMKCPKCNFFVEKIYGCLHITCR 168

[Close]

BLAST of IVF0003254 vs. NCBI nr
Match: XP_008461214.1 (PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo])

HSP 1 Score: 258 bits (660), Expect = 2.09e-85
Identity = 115/119 (96.64%), Postives = 117/119 (98.32%), Query Frame = 0


Query: 66  MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 125
           MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC
Sbjct: 1   MLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCECPLCHRLFCGTC 60

Query: 126 RVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHITCR 184
           RVPWHEGVTCEDYKRLYKYE+ RDEILM+KLASQMKWMKCPKCQ FVEKVSGCLHITCR
Sbjct: 61  RVPWHEGVTCEDYKRLYKYEEIRDEILMEKLASQMKWMKCPKCQLFVEKVSGCLHITCR 119

[Close]

BLAST of IVF0003254 vs. NCBI nr
Match: XP_022146079.1 (probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia])

HSP 1 Score: 245 bits (626), Expect = 9.72e-79
Identity = 111/189 (58.73%), Postives = 139/189 (73.54%), Query Frame = 0


Query: 2   KKLCEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFH 61
           ++LCEICLE K   NMF I+ CLHSFCR CVIKHV+ KI  G S VSC +L+CS  ++F 
Sbjct: 34  RQLCEICLERKAIDNMFTIEACLHSFCRKCVIKHVAAKINGGSSRVSCPSLDCSRFVEFE 93

Query: 62  SCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMI---VERDAKIRDCECPLCH 121
           +CW MLP   AEKW++ALCE ++ E EKV+CPFKDCS  M+     R A I + ECP+CH
Sbjct: 94  TCWGMLPKRVAEKWDRALCEALFVEAEKVMCPFKDCSAAMVNLEKGRSADILESECPICH 153

Query: 122 RLFCGTCRVPWHEGVTCEDYKRLYKYED-GR--DEILMKKLASQMKWMKCPKCQFFVEKV 181
           RLFC  CRVPWH  VTCE+Y+R  K +  GR  D+ L+K +A +M+WM+CP+C+FFVEK 
Sbjct: 154 RLFCAKCRVPWHARVTCEEYQRQKKSKKRGREDDDALLKDMARKMEWMRCPRCKFFVEKT 213

Query: 182 SGCLHITCR 184
            GCLHITCR
Sbjct: 214 YGCLHITCR 222

[Close]

BLAST of IVF0003254 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 7.1e-46
Identity = 75/181 (41.44%), Postives = 110/181 (60.77%), Query Frame = 0


Query: 5   CEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCW 64
           CEIC++ K     F I  C H +C DCV K+++ K+ D   S+ C    CS  ++   C 
Sbjct: 115 CEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILSIECPVSGCSGRLEPDQCR 174

Query: 65  TMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVER-DAKIRDCECPLCHRLFCG 124
            +LP E  ++W  ALCE V    +K  CP+KDCS  + +E  + K++D ECP CHR+ C 
Sbjct: 175 QILPKEVFDRWGDALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRMVCV 234

Query: 125 TCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHITC 184
            C   WH  +TCE++++L   E GRD+IL+  +A Q KW +CP C+F++EK  GCL++ C
Sbjct: 235 ECGTQWHPEMTCEEFQKLAANERGRDDILLATMAKQKKWKRCPSCKFYIEKSQGCLYMKC 294

[Close]

BLAST of IVF0003254 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 3.3e-43
Identity = 71/189 (37.57%), Postives = 115/189 (60.85%), Query Frame = 0


Query: 2   KKLCEICLEYKEEGNMFEIKT-CLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDF 61
           ++LC IC++ K   ++F   T C H++C DC +++V+TKI +  S + C  + C+ +I+ 
Sbjct: 92  RRLCMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKENASRIKCPDVECTRLIEP 151

Query: 62  HSCWTMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVER--DAKIRDCECPLCH 121
           ++C  ++P +  ++W K LCE +    +K  CPFKDCS  M+     DA +   EC  CH
Sbjct: 152 YTCRDLIPKDVFDRWEKILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCH 211

Query: 122 RLFCGTCRVPWHEGVTCEDYKRL---YKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKV 181
           RLFC  C+V WH G+ C++++R     K     D+ L+ ++A   +W +CP C+F+V+KV
Sbjct: 212 RLFCVQCKVTWHAGIGCDEFQRFGNTKKKSSDEDDALLIQMAKNKQWRRCPSCKFYVDKV 271

Query: 182 SGCLHITCR 185
            GC HI CR
Sbjct: 272 EGCQHIKCR 280

[Close]

BLAST of IVF0003254 vs. TAIR 10
Match: AT5G37560.1 (RING/U-box superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 7.8e-29
Identity = 62/186 (33.33%), Postives = 96/186 (51.61%), Query Frame = 0


Query: 5   CEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCW 64
           C IC +  +  +M     CLH  C  CV + V  K+  G     CL   C S +   SC 
Sbjct: 181 CSICSDKTDAEHMLLNDKCLHRHCFSCVKQQVKVKLRSGIVP-PCLEDGCKSELTLESCS 240

Query: 65  TMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCE------CPLCH 124
            +L  +  E W + + E +  + EK+ CP++ CS  M++ +    R+ E      C  C 
Sbjct: 241 MVLTPKLIEMWKRKMEEDLIPDAEKIYCPYRSCS--MLMSKTELSREAEQSNVRACIKCS 300

Query: 125 RLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGC 184
            LFC  C+VPWH  ++C DYKR++  E   +++++K LA+   W +C +C+  +E   GC
Sbjct: 301 ELFCIDCKVPWHSDLSCADYKRIHS-ERLVNDMMLKVLANDQMWRQCSECKHMIELTEGC 360

[Close]

BLAST of IVF0003254 vs. TAIR 10
Match: AT3G45470.1 (IBR domain containing protein )

HSP 1 Score: 124.4 bits (311), Expect = 1.0e-28
Identity = 61/183 (33.33%), Postives = 95/183 (51.91%), Query Frame = 0


Query: 5   CEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCW 64
           C ICLE  +   MF +  CLH  C  CV + V  K+ +G +  +CL   C   +   +C+
Sbjct: 6   CVICLEETKADRMFVMDKCLHRHCYPCVNQLVEVKLRNG-TVPTCLDYECKLKLSLENCF 65

Query: 65  TMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMI---VERDAKIRDCECPLCHRLF 124
            +L  +  E W   + E      +++ CP+ +CS  M    + R  K  D  C  C  L 
Sbjct: 66  KVLKPKVIELWKHMMKEESIPLAKRIYCPYINCSTLMSKTEISRSNKSNDRACIKCSGLV 125

Query: 125 CGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGCLHI 184
           C  C+VPWH  ++C +YK+L+  +   D++ +K LA+  KW +C KC+  +E   GC H+
Sbjct: 126 CIDCKVPWHSDLSCAEYKKLHP-DPVLDDLTLKLLANDQKWRQCVKCRHLIELNQGCNHM 185

[Close]

BLAST of IVF0003254 vs. TAIR 10
Match: AT2G21420.1 (IBR domain containing protein )

HSP 1 Score: 124.0 bits (310), Expect = 1.3e-28
Identity = 67/186 (36.02%), Postives = 98/186 (52.69%), Query Frame = 0


Query: 5   CEICLEYKEEGNMFEIKTCLHSFCRDCVIKHVSTKIGDGYSSVSCLTLNCSSIIDFHSCW 64
           C IC E  +   MF  + C H  C  CV +HV   +  G S  +CL   C+S + F SC 
Sbjct: 187 CCICRENTDADRMFFTENCFHRQCFSCVNRHVQRMLLCGISP-TCLHFPCNSELTFESCS 246

Query: 65  TMLPNETAEKWNKALCEVVYREEEKVVCPFKDCSVGMIVERDAKIRDCE------CPLCH 124
            +L     E W + + E +    +K+ CP++ CS  M++ + A  R+ +      C  C 
Sbjct: 247 KVLTPNLIEFWKRKIEEDLVPAADKIYCPYRRCS--MLMSKTALSRETDQSNVRACIKCC 306

Query: 125 RLFCGTCRVPWHEGVTCEDYKRLYKYEDGRDEILMKKLASQMKWMKCPKCQFFVEKVSGC 184
           RLFC  C+VP H G++C DYK+L    D   ++ +K LA++ KW +C +C   VE   GC
Sbjct: 307 RLFCIDCKVPSHAGLSCVDYKKL--NPDPLYDVKLKSLANKKKWRQCVQCSNLVELFEGC 366

The following BLAST results are available for this feature:

BLAST of IVF0003254 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4KLT0	2.2e-15	24.35	Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ...	[more]
D3YYI7	8.2e-15	25.64	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q8TC41	1.8e-14	25.64	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
A2VEA3	5.9e-13	30.48	E3 ubiquitin-protein ligase ARIH1 OS=Bos taurus OX=9913 GN=ARIH1 PE=2 SV=1	[more]
Q9Y4X5	5.9e-13	30.48	E3 ubiquitin-protein ligase ARIH1 OS=Homo sapiens OX=9606 GN=ARIH1 PE=1 SV=2	[more]

BLAST of IVF0003254 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UU31	2.4e-110	100.00	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A5D3CGK1	3.5e-109	98.91	Putative E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A0A0K9X9	1.4e-94	84.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G430760 PE=3 SV=1	[more]
A0A1S3CDQ5	1.8e-65	96.64	probable E3 ubiquitin-protein ligase RNF217 OS=Cucumis melo OX=3656 GN=LOC103499...	[more]
A0A6J1CX43	7.1e-62	58.73	probable E3 ubiquitin-protein ligase RNF217 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of IVF0003254 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0058684.1	6.00e-143	100.00	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
TYK10490.1	2.01e-141	98.91	putative E3 ubiquitin-protein ligase RNF217 [Cucumis melo var. makuwa]	[more]
KAE8646419.1	1.36e-108	83.93	hypothetical protein Csa_016113 [Cucumis sativus]	[more]
XP_008461214.1	2.09e-85	96.64	PREDICTED: probable E3 ubiquitin-protein ligase RNF217 [Cucumis melo]	[more]
XP_022146079.1	9.72e-79	58.73	probable E3 ubiquitin-protein ligase RNF217 [Momordica charantia]	[more]

BLAST of IVF0003254 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53690.1	7.1e-46	41.44	RING/U-box superfamily protein	[more]
AT3G14250.1	3.3e-43	37.57	RING/U-box superfamily protein	[more]
AT5G37560.1	7.8e-29	33.33	RING/U-box superfamily protein	[more]
AT3G45470.1	1.0e-28	33.33	IBR domain containing protein	[more]
AT2G21420.1	1.3e-28	36.02	IBR domain containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 73..135 e-value: 9.7E-11 score: 51.7
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 75..135 e-value: 1.5E-8 score: 34.7
None	No IPR available	GENE3D	2.20.25.20		coord: 90..136 e-value: 9.5E-6 score: 27.6
None	No IPR available	GENE3D	1.20.120.1750		coord: 137..185 e-value: 3.9E-8 score: 35.5
None	No IPR available	PANTHER	PTHR11685:SF350	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 5..194
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 75..140
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 149..183
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 4..82
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..85 e-value: 1.0E-17 score: 65.8
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 5..44 e-value: 2.9E-7 score: 30.3
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 5..194
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 23..32
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 5..49 score: 8.919052
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 1..199 score: 21.762201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0003254.2	IVF0003254.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity