IVF0003161 (gene) Melon (IVF77) v1

Overview
NameIVF0003161
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionCytochrome P450
Locationchr01: 29953422 .. 29955701 (-)
RNA-Seq ExpressionIVF0003161
SyntenyIVF0003161
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTTATATTGCATGACTATTCAACTTATTCTCCACTTTTTCACAACAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCCATCATGGATTCTGGAAACCTCTCCTCCTCCTCTTCCTCCTCCTTGCCCCCTTCGATTTCCGTCCGACACTTTGGGCTCCAATCGCTCCTCGATGTCCAAATCTTAGAGATCTCTTTTGCCCTTTTTGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACATGGCTTGCCTATATGGCCAATCGTCGGAATGTTACCAGCTTTGATTACGGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTTCAATTTCGAGGTCCGTGGCTTACTAGTCTCAATTGCGTCGTCACTGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTCCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACGGTAGAGTCGTTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGCGGGGAAGTCGACAACGGTGGACTTGCAAGATGTTCTTTTGAGGTTGACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCGGTGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTATTTTCTTTTTCTAGTTTTTTAATTGCATATTCAAATACTTAATTCTCTTTTGTTTGAAAATAGTTTATCATAGAGCTGCCCACCAAAACCTAACTAAATCAGTTAAGATATTACTACCAATCAATGTCACTAAAATTTTCACTCCATAATTCTACAGTAATAAAGTATTGGTTAAATTGTGAGTAAGAAAAAGGGCTAAACTTTGAATGTTATGTGCAATTTCTTATAATATCTATGTATCAAACTATTATTCTTACGAAAAACCCAAAAAAACTATAAATAAATTTAGTTAATTTAATTTTAAGCAGGTACCTGAACATAGGAATTGAGAAGAAGTTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGAACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCCGACAACAAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGAGACGAGCAAGGGCGCCCATTCTCTGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGCAGAAATTTGCAGAATTGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAAGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTAATTATAACAATTACTCAATCACATTCTAAATTCGAAAGTAAACTTCAGAAATCTAATCGATAATCCGTCTGATTCAGGTTGTGGAGGACGATACATTCCCAGACGGAACGATTCTGAAGAAAGGAATGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGATTCATGAGCGAATCGGTGTATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAAGATTTTGCGTATTATCAAATGAAATACGTGGCGGCTTCTATCATCAGCCGGTACCGGGTGGCGGTGGTGGCGGGGCATCCGGTGGAGCCGAAGCTTTCCCTAACTTTCTACATGAAGAAGGGGTTGAAGGTCAATTTGATTAAACGAGAAGAGGGGCAAATTCGGAAATACATGAAGTTAAATTGATTTTAGCCCTTCAAATTAGTGGGAGTGTTAATAAATTTGAAATAATTATACTTTTCTTTTTCTTTTTAATTATTATTGTTTTTAGATTGGAAGAAAAATGTTTGTTTTAAGTGAAAGAGTGAAAAGAAAAATGAG

mRNA sequence

CCTTATATTGCATGACTATTCAACTTATTCTCCACTTTTTCACAACAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCCATCATGGATTCTGGAAACCTCTCCTCCTCCTCTTCCTCCTCCTTGCCCCCTTCGATTTCCGTCCGACACTTTGGGCTCCAATCGCTCCTCGATGTCCAAATCTTAGAGATCTCTTTTGCCCTTTTTGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACATGGCTTGCCTATATGGCCAATCGTCGGAATGTTACCAGCTTTGATTACGGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTTCAATTTCGAGGTCCGTGGCTTACTAGTCTCAATTGCGTCGTCACTGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTCCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACGGTAGAGTCGTTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGCGGGGAAGTCGACAACGGTGGACTTGCAAGATGTTCTTTTGAGGTTGACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCGGTGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTACCTGAACATAGGAATTGAGAAGAAGTTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGAACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCCGACAACAAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGAGACGAGCAAGGGCGCCCATTCTCTGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGCAGAAATTTGCAGAATTGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAAGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATACATTCCCAGACGGAACGATTCTGAAGAAAGGAATGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGATTCATGAGCGAATCGGTGTATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAAGATTTTGCGTATTATCAAATGAAATACGTGGCGGCTTCTATCATCAGCCGGTACCGGGTGGCGGTGGTGGCGGGGCATCCGGTGGAGCCGAAGCTTTCCCTAACTTTCTACATGAAGAAGGGGTTGAAGGTCAATTTGATTAAACGAGAAGAGGGGCAAATTCGGAAATACATGAAGTTAAATTGATTTTAGCCCTTCAAATTAGTGGGAGTGTTAATAAATTTGAAATAATTATACTTTTCTTTTTCTTTTTAATTATTATTGTTTTTAGATTGGAAGAAAAATGTTTGTTTTAAGTGAAAGAGTGAAAAGAAAAATGAG

Coding sequence (CDS)

ATGACTATTCAACTTATTCTCCACTTTTTCACAACAAAAACTTCCTCCATGTTTTCCCATGCATTTCCATTCCTTCATCACAAACCCATCATGGATTCTGGAAACCTCTCCTCCTCCTCTTCCTCCTCCTTGCCCCCTTCGATTTCCGTCCGACACTTTGGGCTCCAATCGCTCCTCGATGTCCAAATCTTAGAGATCTCTTTTGCCCTTTTTGTGTTCTTGGTTATTCATGTTGTTAGACAGAAGAGACGACATGGCTTGCCTATATGGCCAATCGTCGGAATGTTACCAGCTTTGATTACGGCAACACGTGGGAATCTCTACGAGTGGATTACCGACCTCTTGTGTCGTCACAAGGGCACATTTCAATTTCGAGGTCCGTGGCTTACTAGTCTCAATTGCGTCGTCACTGCTGATCCTCGAAACTTAGAACACCTTCTCAAGACAAAATTTTCTTCCTACCCAAAAGGGTCGTTCTTCCGCGACACCGTGAGAGACTTACTTGGTGATGGAATATTCGGGGCTGACGACGAGACATGGCAACGACAGAGGAAGGTGGCGAGCATTGAGTTTCACTCGGCAAGGTTTCGACAAATGACGGTAGAGTCGTTGGTGGAGCTTGTGCACTCGAGGCTGGAAGCAGTGTTGGAGGATTTCGCGGGGAAGTCGACAACGGTGGACTTGCAAGATGTTCTTTTGAGGTTGACGTTTGACAATGTGTGTATGATTGCCTTTGGGGTGGATCCCGGTTGCTTGCGCGGTGGCTTGCCGGAGATTCCATTTGCTAAGGCCTTTGAGGATGCAACTGAAGTCACTGTTATGCGCTTCATCACACCTACATTCATATGGAAAGCCATGAGGTACCTGAACATAGGAATTGAGAAGAAGTTGAAGCAATCGATCAAAGCCGTCGACGAATTTGCGGAGAACGTGATTCAAACACGGAAGAGAGAACTCTCTACGCCGGCGGAATCTCAAGCCGACAACAAAAGATCCGACCTATTAACTGTATTCATGAAGCTAAGAGACGAGCAAGGGCGCCCATTCTCTGATAAGTTTCTCAGAGATATTTGCGTCAATTTCATACTCGCCGGAAGAGACACGTCTTCCGTAGCATTGAGTTGGTTCTTCTGGTTGCTCGATCAAAATCCCGATGTAGAAGATAAAATTCTAGCAGAAATTTGCAGAATTGTCGGCGAACGAGAAGAGGCCAAAACCAGAGAGCTTCGATTCGATTCATTAGTGTTCAAACCAGAAGAGATAAAAAAAATGGAGTATTTGCACGCCGCCATTTCCGAAGCCCTAAGATTGTATCCATCAGTTCCATTCGATCACAAAGAGGTTGTGGAGGACGATACATTCCCAGACGGAACGATTCTGAAGAAAGGAATGAAAATCATCTACGCAATATACGCAATGGGAAGAATGGAAGCGATTTGGGGGAAAGATTGTCGCGAATTCAAACCTGAGAGATGGATGAGAGACGGTCGATTCATGAGCGAATCGGTGTATCGATTCACCGCCTTCAATGGGGGCCCTCGGCTGTGTCTTGGAAAAGATTTTGCGTATTATCAAATGAAATACGTGGCGGCTTCTATCATCAGCCGGTACCGGGTGGCGGTGGTGGCGGGGCATCCGGTGGAGCCGAAGCTTTCCCTAACTTTCTACATGAAGAAGGGGTTGAAGGTCAATTTGATTAAACGAGAAGAGGGGCAAATTCGGAAATACATGAAGTTAAATTGA

Protein sequence

MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN
Homology
BLAST of IVF0003161 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 5.0e-222
Identity = 377/538 (70.07%), Postives = 438/538 (81.41%), Query Frame = 0

Query: 39  SSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPA 98
           SSSSS  P +S R F L+   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 99  LITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGS 158
           L    RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 159 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLED 218
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 219 FAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFIT 278
               S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 279 PTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVF 338
           PT +WK MRYL+IG EKKLK+SIK VD+FA+ VI+TRK+ELS   E+    KRSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVF 316

Query: 339 MKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIV 398
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFFWLL++NP+VE+KI+ E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 399 GEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGT 458
            +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVP DHKEV EDD FPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 459 ILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLG 518
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSES Y+FTAFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 519 KDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKY 577
           KDFAYYQMK  AA+I+ RY+V VV GH VEPKL+LT YMK GL VNLI R   +I +Y
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of IVF0003161 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 6.5e-129
Identity = 242/517 (46.81%), Postives = 334/517 (64.60%), Query Frame = 0

Query: 59  LDVQILEISFALFVFLVIHVVRQKRRH-GLPIWPIVGMLPALITATRGNLYEWITDLLCR 118
           +D     I   L V  +I  V  +R + G  +WP+VG LPALIT     ++++I D L  
Sbjct: 1   MDGSTAAIILTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAH-RMHDFIADNLRM 60

Query: 119 HKGTFQ---FRGPWLTSL--NCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGI 178
             GT+Q   F  P+L     +  VT DP+NLEH+LKT+F +YPKG  ++    DLLGDGI
Sbjct: 61  CGGTYQTCIFPIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGI 120

Query: 179 FGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVL 238
           F +D +TW+ QRK A++EF +   RQ     +   + +RL  +LE    ++  +DLQDVL
Sbjct: 121 FNSDGDTWRFQRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVL 180

Query: 239 LRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIG 298
           LRLTFDN+C + FG DP  L    PE  FA AF+ ATE T+ RFI P FIWK  ++L +G
Sbjct: 181 LRLTFDNICGLTFGKDPRTLSPEFPENGFAVAFDGATEATLQRFIMPEFIWKIRKWLRLG 240

Query: 299 IEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDK 358
           +E  + +SI  VD +   +I TRK EL    + Q +++  DLL+ FMK ++     +SDK
Sbjct: 241 LEDDMSRSISHVDNYLSEIINTRKLELL--GQQQDESRHDDLLSRFMKKKES----YSDK 300

Query: 359 FLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFD 418
           +L+ + +NFILAGRDTSSVA+SWFFWL+  NP VE+KI+ EIC I+ +  +    +   +
Sbjct: 301 YLKYVALNFILAGRDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTDE 360

Query: 419 SLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYA 478
            L F  +EI ++ YL AA+SE LRLYPSVP D K VV +D  PDGT +  G  + Y+IY+
Sbjct: 361 PLTF--DEIDQLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYS 420

Query: 479 MGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 538
           +GRM+ IWG+DC EFKPERW+ + R    + Y+F AFN GPR+CLGKD AY QMK + AS
Sbjct: 421 VGRMKFIWGEDCLEFKPERWLEESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITAS 480

Query: 539 IISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 570
           I+ R+R+ V  GH VE K+SLT +MK GLK+++ KR+
Sbjct: 481 ILLRHRLTVAPGHRVEQKMSLTLFMKFGLKMDVHKRD 508

BLAST of IVF0003161 vs. ExPASy Swiss-Prot
Match: B3RFJ6 (Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1)

HSP 1 Score: 457.2 bits (1175), Expect = 2.7e-127
Identity = 244/491 (49.69%), Postives = 319/491 (64.97%), Query Frame = 0

Query: 86  GLPIWPIVGMLPALITATRGN-LYEWITDLLCRHKGTFQ---FRGPWLTSLNCV-VTADP 145
           G  +WP+VG LP LI    GN ++EWI D L    GT+Q      P+L     V VT DP
Sbjct: 32  GPTMWPVVGSLPGLI--ENGNRMHEWIADNLRACSGTYQTCICAIPFLARKQLVTVTCDP 91

Query: 146 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 205
           +NLEH+LK +F +YPKG  ++    DLLG+GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 92  KNLEHILKVRFDNYPKGPTWQAVFHDLLGEGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 151

Query: 206 VESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 265
              +   + +R   +LE    +   VDLQD+LLRLTFDN+C +AFG DP  L   LPE  
Sbjct: 152 GRWVNRAIKNRFCPILEMAQVQGKPVDLQDLLLRLTFDNICGLAFGKDPETLSPELPENN 211

Query: 266 FAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELS 325
           FA +F+ ATE T+ RFI P F+WK  + L +G+E  L  S+K VD +  +VI TRK EL 
Sbjct: 212 FATSFDRATEATLHRFIMPEFVWKLKKMLGLGLEVSLNNSLKQVDNYMTDVINTRKLELL 271

Query: 326 TPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLL 385
                    +  DLL+ FMK ++     +SDKFL+ + +NFILAGRDTSSVALSWFFWL+
Sbjct: 272 N--HQNGGPQHDDLLSRFMKKKES----YSDKFLQHVALNFILAGRDTSSVALSWFFWLV 331

Query: 386 DQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPS 445
             NP VE+KIL EIC I+ E     T +   + LVF  EE+ ++ YL AA+SE LRLYPS
Sbjct: 332 SSNPRVEEKILVEICTILAETRGNDTSKWLEEPLVF--EEVDQLMYLKAALSETLRLYPS 391

Query: 446 VPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWM-RDG-RF 505
           VP D K V+ DD  PDGT +  G  I Y+IY+ GRM+ IWG+DC EFKPERWM +DG +F
Sbjct: 392 VPEDSKHVISDDYLPDGTFVPAGSNITYSIYSTGRMKFIWGEDCLEFKPERWMSQDGDKF 451

Query: 506 MSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMK 565
             +  +RF AFN GPR+CLGKD AY QMK +AA+++ R+R+AV  GH VE K+SLT +MK
Sbjct: 452 QVQDTFRFVAFNAGPRICLGKDLAYLQMKSIAAAVLLRHRLAVAPGHKVEQKMSLTLFMK 511

Query: 566 KGLKVNLIKRE 570
            GL +N+  R+
Sbjct: 512 DGLVMNVTPRD 512

BLAST of IVF0003161 vs. ExPASy Swiss-Prot
Match: Q9LMM1 (Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 4.7e-127
Identity = 239/492 (48.58%), Postives = 316/492 (64.23%), Query Frame = 0

Query: 86  GLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQ---FRGPWLTSLN--CVVTADP 145
           G  +WP++G LP LI   R  ++EWIT+ L    GT+Q   F  P+L        VT DP
Sbjct: 29  GPRVWPLLGSLPGLI-EQRDRMHEWITENLRACGGTYQTCIFAVPFLAKKQGLVTVTCDP 88

Query: 146 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 205
           +NLEH+LKT+F +YPKG  ++    DLLG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNLEHMLKTRFDNYPKGPTWQSVFHDLLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 206 VESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 265
              +   +  R   +L      +  VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILATAQDNAEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 266 FAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELS 325
           FA AF+ ATE ++ RFI P F+WK  ++L +G+E  L +S+  +DE+   VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFIIPKFMWKLKKWLGLGLEVSLSRSLGEIDEYLAAVINTRKQELM 268

Query: 326 TPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLL 385
           +  ES    +  DLL+ FM  + E    +SD FL+ + +NFILAGRDTSSVALSWFFWL+
Sbjct: 269 SQQESGTHQRHDDLLSRFMMKKTES---YSDTFLQHVALNFILAGRDTSSVALSWFFWLI 328

Query: 386 DQNPDVEDKILAEICRIVGE-REEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYP 445
             +P VEDKI+ EIC ++ E R          + L F  +EI ++ YL AAISE LRLYP
Sbjct: 329 TMHPTVEDKIVREICSVLIETRGTDDVASWTEEPLGF--DEIDRLVYLKAAISETLRLYP 388

Query: 446 SVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMR--DGR 505
           SVP D K V  DD  PDGT +  G  + Y+IYA GRM++ WG+DC EF PERW+   DG+
Sbjct: 389 SVPEDSKHVENDDVLPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFNPERWISPIDGK 448

Query: 506 FMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYM 565
           F++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ VV GH VE K+SLT +M
Sbjct: 449 FINHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVVPGHKVEQKMSLTLFM 508

Query: 566 KKGLKVNLIKRE 570
           K GL VNL KR+
Sbjct: 509 KNGLLVNLYKRD 514

BLAST of IVF0003161 vs. ExPASy Swiss-Prot
Match: O23066 (Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1)

HSP 1 Score: 451.1 bits (1159), Expect = 2.0e-125
Identity = 232/491 (47.25%), Postives = 316/491 (64.36%), Query Frame = 0

Query: 86  GLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQ---FRGPWLTSLN--CVVTADP 145
           G  +WP++G LP LI   R  +++WIT+ L    GT+Q      P+L        VT DP
Sbjct: 29  GPRVWPVLGSLPGLI-EQRDRMHDWITENLRACGGTYQTCICAVPFLAKKQGLVTVTCDP 88

Query: 146 RNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMT 205
           +N+EH+LKT+F +YPKG  ++    D LG GIF +D +TW  QRK A++EF +   RQ  
Sbjct: 89  KNIEHMLKTRFDNYPKGPTWQAVFHDFLGQGIFNSDGDTWLFQRKTAALEFTTRTLRQAM 148

Query: 206 VESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIP 265
              +   +  R   +LE        VDLQD++LRLTFDN+C +AFG D      GLPE  
Sbjct: 149 GRWVNRGIKLRFCPILETAQNNYEPVDLQDLILRLTFDNICGLAFGKDTRTCAPGLPENG 208

Query: 266 FAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELS 325
           FA AF+ ATE ++ RFI P F+W+  ++L +G+E  L +S+  +D + + VI TRK+EL 
Sbjct: 209 FASAFDRATEASLQRFILPEFLWRLKKWLGLGLEVSLSRSLGEIDGYLDAVINTRKQELL 268

Query: 326 TPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLL 385
           +  ES    +  DLL+ FMK +D+    +S+ FLR + +NFILAGRDTSSVALSWFFWL+
Sbjct: 269 SQRESGV-QRHDDLLSRFMKKKDQS---YSETFLRHVALNFILAGRDTSSVALSWFFWLI 328

Query: 386 DQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPS 445
             +P VEDKI+ EIC ++ E           + L F  +E+ ++ YL AA+SE LRLYPS
Sbjct: 329 TTHPTVEDKIVREICSVLIETRGTDVSSWTAEPLEF--DEVDRLVYLKAALSETLRLYPS 388

Query: 446 VPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMR--DGRF 505
           VP D K VV DD  PDGT +  G  + Y+IYA GRM++ WG+DC EFKPERW+   DG+F
Sbjct: 389 VPEDSKHVVNDDILPDGTFVPAGSSVTYSIYAAGRMKSTWGEDCLEFKPERWISPDDGKF 448

Query: 506 MSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMK 565
           ++   YRF AFN GPR+CLGKD AY QMK +AA+++ R+R+ V  GH VE K+SLT +MK
Sbjct: 449 VNHDQYRFVAFNAGPRICLGKDLAYLQMKTIAAAVLLRHRLTVAPGHKVEQKMSLTLFMK 508

Query: 566 KGLKVNLIKRE 570
            GL VN+ KR+
Sbjct: 509 NGLLVNVHKRD 512

BLAST of IVF0003161 vs. ExPASy TrEMBL
Match: A0A1S3CEY8 (LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103499965 PE=3 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 578/580 (99.66%), Postives = 578/580 (99.66%), Query Frame = 0

Query: 1   MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD 60
           MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD
Sbjct: 9   MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD 68

Query: 61  VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG 120
           VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG
Sbjct: 69  VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG 128

Query: 121 TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW 180
           TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW
Sbjct: 129 TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW 188

Query: 181 QRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV 240
           QRQRKVASIEFHSARFRQMTVESLVEL HSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV
Sbjct: 189 QRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV 248

Query: 241 CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS 300
           CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS
Sbjct: 249 CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS 308

Query: 301 IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN 360
           IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN
Sbjct: 309 IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN 368

Query: 361 FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE 420
           FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE
Sbjct: 369 FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE 428

Query: 421 IKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW 480
            KKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW
Sbjct: 429 XKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW 488

Query: 481 GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA 540
           GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA
Sbjct: 489 GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA 548

Query: 541 VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 581
           VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN
Sbjct: 549 VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of IVF0003161 vs. ExPASy TrEMBL
Match: A0A5A7UTT4 (Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G00200 PE=3 SV=1)

HSP 1 Score: 1104.4 bits (2855), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 31  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 90
           MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 91  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 150
           PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 151 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 210
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

Query: 211 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 270
           RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE
Sbjct: 181 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 240

Query: 271 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 330
           VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK
Sbjct: 241 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 300

Query: 331 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 390
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI
Sbjct: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 360

Query: 391 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 450
           LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 361 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420

Query: 451 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 510
           DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN
Sbjct: 421 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 480

Query: 511 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 570
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE
Sbjct: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 540

Query: 571 GQIRKYMKLN 581
           GQIRKYMKLN
Sbjct: 541 GQIRKYMKLN 550

BLAST of IVF0003161 vs. ExPASy TrEMBL
Match: A0A0A0K879 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.7e-303
Identity = 522/551 (94.74%), Postives = 537/551 (97.46%), Query Frame = 0

Query: 31  MDSGNL-SSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPI 90
           MDSGNL SSSSSSS  P IS+ HFGL+SLLDVQILEI+FALFVFLVIHVVRQKRRHGLPI
Sbjct: 1   MDSGNLSSSSSSSSFSPPISIGHFGLRSLLDVQILEITFALFVFLVIHVVRQKRRHGLPI 60

Query: 91  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 150
           WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT
Sbjct: 61  WPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKT 120

Query: 151 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 210
           KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH
Sbjct: 121 KFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVH 180

Query: 211 SRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDAT 270
           SRLEAVLEDF G++TTVDLQDVLLRLTFDNVCMIAFGVDPGCLR GLPEIPFAKAFEDAT
Sbjct: 181 SRLEAVLEDFEGQATTVDLQDVLLRLTFDNVCMIAFGVDPGCLRSGLPEIPFAKAFEDAT 240

Query: 271 EVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADN 330
           EVTV+RFITPTFIWK MRYLNIGIEKKLKQSIKAVDEFAE+VIQTRKRELSTPAESQA+N
Sbjct: 241 EVTVIRFITPTFIWKFMRYLNIGIEKKLKQSIKAVDEFAEDVIQTRKRELSTPAESQAEN 300

Query: 331 KRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDK 390
           +RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFF LLDQNPDVEDK
Sbjct: 301 QRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFLLLDQNPDVEDK 360

Query: 391 ILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVV 450
           IL EICRIVGEREEAKTRELRFDSLVFKPEEIK+MEYLHAAISEALRLYPSVPFDHKEVV
Sbjct: 361 ILEEICRIVGEREEAKTRELRFDSLVFKPEEIKRMEYLHAAISEALRLYPSVPFDHKEVV 420

Query: 451 EDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAF 510
           EDD FPDGT+LKKG KIIYAIYAMGRMEAIWGKDCREFKPERWMR+GRFMSES YRFTAF
Sbjct: 421 EDDVFPDGTVLKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMREGRFMSESAYRFTAF 480

Query: 511 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 570
           NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFYMKKGLKVNLIKRE
Sbjct: 481 NGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVRGHPVEPKLALTFYMKKGLKVNLIKRE 540

Query: 571 EGQIRKYMKLN 581
           EGQ+RKY+KLN
Sbjct: 541 EGQLRKYIKLN 551

BLAST of IVF0003161 vs. ExPASy TrEMBL
Match: A0A6J1GQ36 (cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=1)

HSP 1 Score: 1024.6 bits (2648), Expect = 1.6e-295
Identity = 505/564 (89.54%), Postives = 531/564 (94.15%), Query Frame = 0

Query: 17  MFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVI 76
           MFSHA PFLHHKP+MDSGNL  SSSS+LP  +S  +FGL  LLD QILEISFALFVFL I
Sbjct: 1   MFSHAIPFLHHKPMMDSGNL--SSSSALPALVSAGNFGLWPLLDFQILEISFALFVFLAI 60

Query: 77  HVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVV 136
           HVVRQ+RR+GLP+WPIVGMLP+LITA RGNLYEWITDLL  HKGTFQFRGPW +SLNCVV
Sbjct: 61  HVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRHHKGTFQFRGPWFSSLNCVV 120

Query: 137 TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 196
           TADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF
Sbjct: 121 TADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 180

Query: 197 RQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 256
           RQMTVESLVELVHSRLE VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL
Sbjct: 181 RQMTVESLVELVHSRLETVLEDFLGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 240

Query: 257 PEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRK 316
           PEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAE+VIQTRK
Sbjct: 241 PEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTRK 300

Query: 317 RELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 376
           RELST A+ QA+N+RSDLLTVFM+LRDEQ RPFSDKFLRDICVNFILAGRDTSSVALSWF
Sbjct: 301 RELSTTAQPQAENQRSDLLTVFMRLRDEQRRPFSDKFLRDICVNFILAGRDTSSVALSWF 360

Query: 377 FWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALR 436
           FWLLD+NPDVEDKILAEI RIVGEREEAKTRE RFDSLVFKPEEIKKMEYLHAAISEALR
Sbjct: 361 FWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALR 420

Query: 437 LYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDG 496
           LYPSVP DHKEVVEDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWM+DG
Sbjct: 421 LYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDG 480

Query: 497 RFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFY 556
           RFMSES YRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFY
Sbjct: 481 RFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFY 540

Query: 557 MKKGLKVNLIKREEGQIRKYMKLN 581
           MK GLKVNLIKREEGQIRKY+KLN
Sbjct: 541 MKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of IVF0003161 vs. ExPASy TrEMBL
Match: A0A6J1JMG1 (cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.8e-294
Identity = 504/564 (89.36%), Postives = 529/564 (93.79%), Query Frame = 0

Query: 17  MFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVI 76
           MFSHA PFLHHKPIMDSGNL  SSSS+LP  +S  +F L  LLDVQILE+SFALFVFL I
Sbjct: 1   MFSHAIPFLHHKPIMDSGNL--SSSSALPAVVSAENFRLWPLLDVQILELSFALFVFLAI 60

Query: 77  HVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVV 136
           HV+RQ+RR+GL +WPIVGMLP+LITA RGNLYEWITDLL RHKGTFQFRGPW +SLNCVV
Sbjct: 61  HVIRQQRRYGLSVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCVV 120

Query: 137 TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 196
           TADPRNLEHLLKTKF  YPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF
Sbjct: 121 TADPRNLEHLLKTKFPYYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 180

Query: 197 RQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 256
           RQMTVESLVELVHSRLE VLEDF  + T VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL
Sbjct: 181 RQMTVESLVELVHSRLETVLEDFVERRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 240

Query: 257 PEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRK 316
           PEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSIK VDEFAE+VIQTRK
Sbjct: 241 PEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIKTVDEFAEDVIQTRK 300

Query: 317 RELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 376
           RELST A+ QA+N+RSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF
Sbjct: 301 RELSTTAQPQANNQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 360

Query: 377 FWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALR 436
           FWLLDQNPDVE+KILAEI RIVGER EAKTRE RFDSLVFKPEEIKKMEYLHAAISEALR
Sbjct: 361 FWLLDQNPDVENKILAEIYRIVGERGEAKTREFRFDSLVFKPEEIKKMEYLHAAISEALR 420

Query: 437 LYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDG 496
           LYPSVP DHKEVVEDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWM+DG
Sbjct: 421 LYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKDG 480

Query: 497 RFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFY 556
           RFMSES YRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTFY
Sbjct: 481 RFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTFY 540

Query: 557 MKKGLKVNLIKREEGQIRKYMKLN 581
           MK GLKVNLIKREEGQIRKYMKLN
Sbjct: 541 MKDGLKVNLIKREEGQIRKYMKLN 562

BLAST of IVF0003161 vs. NCBI nr
Match: XP_008461361.1 (PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo])

HSP 1 Score: 1155 bits (2989), Expect = 0.0
Identity = 578/580 (99.66%), Postives = 578/580 (99.66%), Query Frame = 0

Query: 1   MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD 60
           MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD
Sbjct: 9   MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLD 68

Query: 61  VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG 120
           VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG
Sbjct: 69  VQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKG 128

Query: 121 TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW 180
           TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW
Sbjct: 129 TFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETW 188

Query: 181 QRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV 240
           QRQRKVASIEFHSARFRQMTVESLVEL HSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV
Sbjct: 189 QRQRKVASIEFHSARFRQMTVESLVELAHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNV 248

Query: 241 CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS 300
           CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS
Sbjct: 249 CMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQS 308

Query: 301 IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN 360
           IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN
Sbjct: 309 IKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVN 368

Query: 361 FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE 420
           FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE
Sbjct: 369 FILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEE 428

Query: 421 IKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW 480
            KKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW
Sbjct: 429 XKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIW 488

Query: 481 GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA 540
           GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA
Sbjct: 489 GKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVA 548

Query: 541 VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 580
           VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN
Sbjct: 549 VVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 588

BLAST of IVF0003161 vs. NCBI nr
Match: XP_004135954.3 (cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_015511 [Cucumis sativus])

HSP 1 Score: 1102 bits (2850), Expect = 0.0
Identity = 551/581 (94.84%), Postives = 566/581 (97.42%), Query Frame = 0

Query: 1   MTIQLILHFFTTKTSSMFSHAFPFLHHKPIMDSGNLSSSSSSS-LPPSISVRHFGLQSLL 60
           MTIQLILHFFTT TSSMFSHAFPFLHHKPIMDSGNLSSSSSSS   P IS+ HFGL+SLL
Sbjct: 9   MTIQLILHFFTTITSSMFSHAFPFLHHKPIMDSGNLSSSSSSSSFSPPISIGHFGLRSLL 68

Query: 61  DVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHK 120
           DVQILEI+FALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHK
Sbjct: 69  DVQILEITFALFVFLVIHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHK 128

Query: 121 GTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDET 180
           GTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDET
Sbjct: 129 GTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDET 188

Query: 181 WQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDN 240
           WQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDF G++TTVDLQDVLLRLTFDN
Sbjct: 189 WQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFEGQATTVDLQDVLLRLTFDN 248

Query: 241 VCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQ 300
           VCMIAFGVDPGCLR GLPEIPFAKAFEDATEVTV+RFITPTFIWK MRYLNIGIEKKLKQ
Sbjct: 249 VCMIAFGVDPGCLRSGLPEIPFAKAFEDATEVTVIRFITPTFIWKFMRYLNIGIEKKLKQ 308

Query: 301 SIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICV 360
           SIKAVDEFAE+VIQTRKRELSTPAESQA+N+RSDLLTVFMKLRDEQGRPFSDKFLRDICV
Sbjct: 309 SIKAVDEFAEDVIQTRKRELSTPAESQAENQRSDLLTVFMKLRDEQGRPFSDKFLRDICV 368

Query: 361 NFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPE 420
           NFILAGRDTSSVALSWFF LLDQNPDVEDKIL EICRIVGEREEAKTRELRFDSLVFKPE
Sbjct: 369 NFILAGRDTSSVALSWFFLLLDQNPDVEDKILEEICRIVGEREEAKTRELRFDSLVFKPE 428

Query: 421 EIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAI 480
           EIK+MEYLHAAISEALRLYPSVPFDHKEVVEDD FPDGT+LKKG KIIYAIYAMGRMEAI
Sbjct: 429 EIKRMEYLHAAISEALRLYPSVPFDHKEVVEDDVFPDGTVLKKGTKIIYAIYAMGRMEAI 488

Query: 481 WGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRV 540
           WGKDCREFKPERWMR+GRFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRV
Sbjct: 489 WGKDCREFKPERWMREGRFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRV 548

Query: 541 AVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKYMKLN 580
           AVV GHPVEPKL+LTFYMKKGLKVNLIKREEGQ+RKY+KLN
Sbjct: 549 AVVRGHPVEPKLALTFYMKKGLKVNLIKREEGQLRKYIKLN 589

BLAST of IVF0003161 vs. NCBI nr
Match: KAA0058578.1 (cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450 86B1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1100 bits (2844), Expect = 0.0
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0

Query: 31  MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 90
           MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW
Sbjct: 1   MDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIW 60

Query: 91  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 150
           PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK
Sbjct: 61  PIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTK 120

Query: 151 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 210
           FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS
Sbjct: 121 FSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHS 180

Query: 211 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 270
           RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE
Sbjct: 181 RLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATE 240

Query: 271 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 330
           VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK
Sbjct: 241 VTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNK 300

Query: 331 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 390
           RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI
Sbjct: 301 RSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKI 360

Query: 391 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 450
           LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE
Sbjct: 361 LAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVE 420

Query: 451 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 510
           DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN
Sbjct: 421 DDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFN 480

Query: 511 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 570
           GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE
Sbjct: 481 GGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREE 540

Query: 571 GQIRKYMKLN 580
           GQIRKYMKLN
Sbjct: 541 GQIRKYMKLN 550

BLAST of IVF0003161 vs. NCBI nr
Match: XP_038899430.1 (cytochrome P450 86B1-like [Benincasa hispida])

HSP 1 Score: 1026 bits (2653), Expect = 0.0
Identity = 512/564 (90.78%), Postives = 533/564 (94.50%), Query Frame = 0

Query: 17  MFSHAFPFLHHKPIMDSGNLSSSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVI 76
           MFSHA PFLHHKP+MDSGNLSS SSS LPP ISV +F L+ LLDVQILEISFALFVFLVI
Sbjct: 1   MFSHACPFLHHKPMMDSGNLSS-SSSPLPPPISVANFRLRPLLDVQILEISFALFVFLVI 60

Query: 77  HVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVV 136
           HVVRQKRR+ LP+WP+VGMLPALITA RGN+YEWIT+LL RHKGTFQFRGPWL+SL+CVV
Sbjct: 61  HVVRQKRRYDLPVWPLVGMLPALITAARGNIYEWITELLHRHKGTFQFRGPWLSSLHCVV 120

Query: 137 TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 196
           TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF
Sbjct: 121 TADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARF 180

Query: 197 RQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 256
           RQMTV+SLVELVHSRLE VLE+FAG+S  VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL
Sbjct: 181 RQMTVDSLVELVHSRLEPVLENFAGRSEVVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGL 240

Query: 257 PEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRK 316
           PEIPFAKAFEDATEVTV+RFITPTFIWK MRYLN+G+EKKLKQSIKAVDEFAENVIQTRK
Sbjct: 241 PEIPFAKAFEDATEVTVLRFITPTFIWKVMRYLNLGMEKKLKQSIKAVDEFAENVIQTRK 300

Query: 317 RELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 376
           RE  T A+ QAD++RSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF
Sbjct: 301 REFYTAAQPQADDQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWF 360

Query: 377 FWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALR 436
           FWLLDQNPDVE KIL EICRIV EREEAKTRELR DSLVFKPEEIKKMEYLHAAISEALR
Sbjct: 361 FWLLDQNPDVEHKILEEICRIVSEREEAKTRELRLDSLVFKPEEIKKMEYLHAAISEALR 420

Query: 437 LYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDG 496
           LYPSVP DHKEVVEDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDC EFKPERWMRDG
Sbjct: 421 LYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCCEFKPERWMRDG 480

Query: 497 RFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFY 556
           RFMSES YRFTAFNGGPRLCLGKDFAYYQMKYVAAS ISRYRVAVV GHPVEPKL+LTFY
Sbjct: 481 RFMSESAYRFTAFNGGPRLCLGKDFAYYQMKYVAASTISRYRVAVVTGHPVEPKLALTFY 540

Query: 557 MKKGLKVNLIKREEGQIRKYMKLN 580
           MK GLKVNLIKREEGQI KYMKLN
Sbjct: 541 MKNGLKVNLIKREEGQIWKYMKLN 563

BLAST of IVF0003161 vs. NCBI nr
Match: KAG7014270.1 (Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1021 bits (2639), Expect = 0.0
Identity = 505/565 (89.38%), Postives = 533/565 (94.34%), Query Frame = 0

Query: 17  MFSHAFPFLHHKPIMDSGNLSSSSSSSLPPS-ISVRHFGLQSLLDVQILEISFALFVFLV 76
           MFSHA PFLHHKP+MDSGNL   S+SS PP+ +S  +FGL  LLD QILEISFALF+FL 
Sbjct: 1   MFSHAIPFLHHKPMMDSGNL---STSSAPPALVSAGNFGLWPLLDFQILEISFALFMFLA 60

Query: 77  IHVVRQKRRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCV 136
           IHVVRQ+RR+GLP+WPIVGMLP+LITA RGNLYEWITDLL RHKGTFQFRGPW +SLNCV
Sbjct: 61  IHVVRQQRRYGLPVWPIVGMLPSLITAARGNLYEWITDLLRRHKGTFQFRGPWFSSLNCV 120

Query: 137 VTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 196
           VTADPRNLEHLLKTKF SYPKG FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR
Sbjct: 121 VTADPRNLEHLLKTKFPSYPKGPFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSAR 180

Query: 197 FRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 256
           FRQMTVESLVELVHSRLE VLEDF G+ T VDLQDVLLRLTFDNVCMIAFGVDPGCLRGG
Sbjct: 181 FRQMTVESLVELVHSRLETVLEDFVGRRTAVDLQDVLLRLTFDNVCMIAFGVDPGCLRGG 240

Query: 257 LPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTR 316
           LPEIPFAKAFEDATEVTV+RFITPTF+WK +RYLN+G+EKKLKQSI+AVDEFAE+VIQTR
Sbjct: 241 LPEIPFAKAFEDATEVTVLRFITPTFVWKILRYLNLGVEKKLKQSIQAVDEFAEDVIQTR 300

Query: 317 KRELSTPAESQADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 376
           KRELST A+ QA+N+RSDLLTVFM+LRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW
Sbjct: 301 KRELSTTAQPQAENQRSDLLTVFMRLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSW 360

Query: 377 FFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEAL 436
           FFWLLD+NPDVEDKILAEI RIVGEREEAKTRE RFDSLVFKPEEIKKMEYLHAAISEAL
Sbjct: 361 FFWLLDRNPDVEDKILAEIYRIVGEREEAKTREFRFDSLVFKPEEIKKMEYLHAAISEAL 420

Query: 437 RLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRD 496
           RLYPSVP DHKEVVEDD FPDGTILKKG KIIYAIYAMGRMEAIWGKDCREFKPERWM+D
Sbjct: 421 RLYPSVPVDHKEVVEDDVFPDGTILKKGTKIIYAIYAMGRMEAIWGKDCREFKPERWMKD 480

Query: 497 GRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTF 556
           GRFMSES YRFTAFNGGPRLCLG+DFAYYQMKYVAASIISRYRVAVV GHPVEPKL+LTF
Sbjct: 481 GRFMSESAYRFTAFNGGPRLCLGRDFAYYQMKYVAASIISRYRVAVVKGHPVEPKLALTF 540

Query: 557 YMKKGLKVNLIKREEGQIRKYMKLN 580
           YMK GLKVNLIKREEGQIRKY+KLN
Sbjct: 541 YMKDGLKVNLIKREEGQIRKYVKLN 562

BLAST of IVF0003161 vs. TAIR 10
Match: AT5G23190.1 (cytochrome P450, family 86, subfamily B, polypeptide 1 )

HSP 1 Score: 771.9 bits (1992), Expect = 3.6e-223
Identity = 377/538 (70.07%), Postives = 438/538 (81.41%), Query Frame = 0

Query: 39  SSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPIWPIVGMLPA 98
           SSSSS  P +S R F L+   DVQILE+  A+FVF+ IH +RQK+  GLP+WP +GMLP+
Sbjct: 17  SSSSSSDPLVSRRLFLLR---DVQILELLIAIFVFVAIHALRQKKYQGLPVWPFLGMLPS 76

Query: 99  LITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGS 158
           L    RGN+YEW++D+LC   GTFQFRGPW +SLN  +T DPRN+EHLLK +FS +PKGS
Sbjct: 77  LAFGLRGNIYEWLSDVLCLQNGTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGS 136

Query: 159 FFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLED 218
           +FRD +RDLLGDGIF ADDETWQRQRK ASIEFHSA+FRQ+T +SL ELVH RL  VLE 
Sbjct: 137 YFRDNLRDLLGDGIFNADDETWQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLET 196

Query: 219 FAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFIT 278
               S+ +DLQDVLLRLTFDNVCMIAFGVDPGCL    P IPFAKAFEDATE  V+RF+ 
Sbjct: 197 SVKSSSPIDLQDVLLRLTFDNVCMIAFGVDPGCLGPDQPVIPFAKAFEDATEAAVVRFVM 256

Query: 279 PTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVF 338
           PT +WK MRYL+IG EKKLK+SIK VD+FA+ VI+TRK+ELS   E+    KRSDLLTVF
Sbjct: 257 PTCVWKFMRYLDIGTEKKLKESIKGVDDFADEVIRTRKKELSLEGET---TKRSDLLTVF 316

Query: 339 MKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIV 398
           M LRDE+G  FSDKFLRDICVNFILAGRDTSSVALSWFFWLL++NP+VE+KI+ E+C+I+
Sbjct: 317 MGLRDEKGESFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKIL 376

Query: 399 GEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGT 458
            +R++    E      VF PEEIKKM+YL AA+SEALRLYPSVP DHKEV EDD FPDGT
Sbjct: 377 RQRDDHGNAEKSDYEPVFGPEEIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGT 436

Query: 459 ILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLG 518
           +LKKG K+IYAIYAMGRMEAIWGKDC EF+PERW+RDGRFMSES Y+FTAFNGGPRLCLG
Sbjct: 437 MLKKGDKVIYAIYAMGRMEAIWGKDCLEFRPERWLRDGRFMSESAYKFTAFNGGPRLCLG 496

Query: 519 KDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKREEGQIRKY 577
           KDFAYYQMK  AA+I+ RY+V VV GH VEPKL+LT YMK GL VNLI R   +I +Y
Sbjct: 497 KDFAYYQMKSTAAAIVYRYKVKVVNGHKVEPKLALTMYMKHGLMVNLINRSVSEIDQY 548

BLAST of IVF0003161 vs. TAIR 10
Match: AT5G08250.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 722.2 bits (1863), Expect = 3.2e-208
Identity = 346/493 (70.18%), Postives = 410/493 (83.16%), Query Frame = 0

Query: 87  LPIWPIVGMLPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHL 146
           +P+WP+VGMLP+LI+A R N+YEW++D+L    GTF+FRGPW ++LNCVVT DPRN+EHL
Sbjct: 1   MPVWPLVGMLPSLISAVRSNIYEWLSDVLISQNGTFRFRGPWFSTLNCVVTCDPRNVEHL 60

Query: 147 LKTKFSSYPKGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVE 206
           LKT+FS YPKGS+FR+T++DLLGDGIF  DD TWQRQRK AS+EFHSA+FRQ+T +SL E
Sbjct: 61  LKTRFSIYPKGSYFRETMQDLLGDGIFNTDDGTWQRQRKAASVEFHSAKFRQLTSQSLHE 120

Query: 207 LVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFE 266
           LVH+RL  VLE     S  +DLQD+LLRLTFDNVCMIAFGVDPGCL   LPEIPFAKAFE
Sbjct: 121 LVHNRLLPVLE----TSGKIDLQDILLRLTFDNVCMIAFGVDPGCLSPKLPEIPFAKAFE 180

Query: 267 DATEVTVMRFITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQ 326
           DATE TV+RF+ P F+WK MR LN+G EKKLK+SI  VD+FAE VI+TRK+E+S   E  
Sbjct: 181 DATEATVVRFVMPKFVWKLMRSLNLGTEKKLKESINGVDDFAEEVIRTRKKEMSLETEIA 240

Query: 327 ADNKRSDLLTVFMKLRDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDV 386
              KR DLLT+FM LRDE G+ FSDKFLRDICVNFILAGRDTSSVALSWFFWL+++NP+V
Sbjct: 241 ---KRPDLLTIFMGLRDENGQKFSDKFLRDICVNFILAGRDTSSVALSWFFWLIEKNPEV 300

Query: 387 EDKILAEICRIVGER-EEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDH 446
           E+KI+  IC+I+ +R +   T++      VF+PEEIKKM+YL AA+SE LRLYPSVP DH
Sbjct: 301 EEKIMMGICKILEQRVDHGDTKKNMEYEPVFRPEEIKKMDYLQAALSETLRLYPSVPVDH 360

Query: 447 KEVVEDDTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYR 506
           KEV+EDD FPDGT LKKG K+IYAIYAMGRME IWGKDCREFKPERW+RDGR+MSES Y+
Sbjct: 361 KEVLEDDVFPDGTKLKKGEKVIYAIYAMGRMETIWGKDCREFKPERWLRDGRYMSESAYK 420

Query: 507 FTAFNGGPRLCLGKDFAYYQMKYVAASIISRYRVAV--VAGHPVEPKLSLTFYMKKGLKV 566
           FTAFNGGPRLCLGKDFAYYQM+YVAA+II RY+V V    GH VEPK++LT YMK GLKV
Sbjct: 421 FTAFNGGPRLCLGKDFAYYQMRYVAAAIIYRYKVRVDDKGGHKVEPKMALTMYMKHGLKV 480

Query: 567 NLIKREEGQIRKY 577
           N++KR   +I  Y
Sbjct: 481 NMVKRSVSEIDHY 486

BLAST of IVF0003161 vs. TAIR 10
Match: AT1G24540.1 (cytochrome P450, family 86, subfamily C, polypeptide 1 )

HSP 1 Score: 497.7 bits (1280), Expect = 1.3e-140
Identity = 241/517 (46.62%), Postives = 349/517 (67.50%), Query Frame = 0

Query: 61  VQILEISFALFVFLVIHVVRQK--RRHGLPIWPIVGMLPALITATRGNLYEWITDLLCRH 120
           +++ +++ AL    ++  +R+K   + G  +WP++G++P ++   + +L+ W T  + R 
Sbjct: 15  LRLSDVALALVGLFLLSYLREKLVSKGGPVMWPVLGIIP-MLALNKHDLFTWCTRCVVRS 74

Query: 121 KGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVRDLLGDGIFGADDE 180
            GTF +RG W      ++TADP N+EH+LKT F +YPKG+F+R+  RDLL DGIF ADDE
Sbjct: 75  GGTFHYRGIWFGGAYGIMTADPANVEHILKTNFKNYPKGAFYRERFRDLLEDGIFNADDE 134

Query: 181 TWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTTVDLQDVLLRLTFD 240
            W+ +R+VA  E HS+RF + T  ++ +LV  +L  ++E+ +      DLQD+LLR TFD
Sbjct: 135 LWKEERRVAKTEMHSSRFLEHTFTTMRDLVDQKLVPLMENLSTSKRVFDLQDLLLRFTFD 194

Query: 241 NVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKAMRYLNIGIEKKLK 300
           N+C+ AFGV PG L  GLPEIPFAKAFEDATE T+ RF+ P F+WK MR+L IG E+KL 
Sbjct: 195 NICISAFGVYPGSLETGLPEIPFAKAFEDATEYTLARFLIPPFVWKPMRFLGIGYERKLN 254

Query: 301 QSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKLRDE------QGRPFSDK 360
            +++ V  FA   ++ R+ ++         N  +DLL+  M+   E      +G  FSDK
Sbjct: 255 NAVRIVHAFANKTVRERRNKMRKLGNL---NDYADLLSRLMQREYEKEEDTTRGNYFSDK 314

Query: 361 FLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGEREEAKTRELRFD 420
           + R+ C +FI+AGRDT+SVAL WFFWL+ ++P+VE +IL EI  I  +    +T +    
Sbjct: 315 YFREFCTSFIIAGRDTTSVALVWFFWLVQKHPEVEKRILREIREIKRKLTTQETED---- 374

Query: 421 SLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDGTILKKGMKIIYAIYA 480
              F+ E+ ++M YL AA++E+LRLYPSVP + K+ +EDD  PDGT +KKG +I Y++Y+
Sbjct: 375 --QFEAEDFREMVYLQAALTESLRLYPSVPMEMKQALEDDVLPDGTRVKKGARIHYSVYS 434

Query: 481 MGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCLGKDFAYYQMKYVAAS 540
           MGR+E+IWGKD  EFKPERW+++GR +SE  +++  FNGGPRLC+GK FAY QMK VAA+
Sbjct: 435 MGRIESIWGKDWEEFKPERWIKEGRIVSEDQFKYVVFNGGPRLCVGKKFAYTQMKMVAAA 494

Query: 541 IISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKRE 570
           I+ RY V VV G  + PKL+ T YMK G+ V L  R+
Sbjct: 495 ILMRYSVKVVQGQEIVPKLTTTLYMKNGMNVMLQPRD 521

BLAST of IVF0003161 vs. TAIR 10
Match: AT1G13140.1 (cytochrome P450, family 86, subfamily C, polypeptide 3 )

HSP 1 Score: 472.2 bits (1214), Expect = 5.8e-133
Identity = 237/534 (44.38%), Postives = 344/534 (64.42%), Query Frame = 0

Query: 38  SSSSSSLPPSISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQK--RRHGLPIWPIVGM 97
           S  SSS+P  ++ R +    L DV     S AL    V   VR+K  ++ G  IWP+ G+
Sbjct: 2   SEISSSMP--LTERVYNHLCLSDV-----SLALLGLFVFCCVREKVTKKLGPTIWPVFGI 61

Query: 98  LPALITATRGNLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYP 157
            P      R ++Y W T  L + +GTF + G WL      VT  P N+E++LKT F ++P
Sbjct: 62  TPEFF-FHRNDVYGWATRCLKKCRGTFLYNGIWLGGSYGAVTCVPANVEYMLKTNFKNFP 121

Query: 158 KGSFFRDTVRDLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAV 217
           KG+FF++   DLL DGIF AD E+W+ QR++   E HS RF + + ++  +LV  +L  V
Sbjct: 122 KGAFFKERFNDLLEDGIFNADAESWKEQRRIIITEMHSTRFVEHSFQTTQDLVRKKLLKV 181

Query: 218 LEDFAGKSTTVDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMR 277
           +E FA      DLQDVLLRLTFDN+C+   G DPG L   LP +PFA+AFE+ATE T+ R
Sbjct: 182 MESFARSQEAFDLQDVLLRLTFDNICIAGLGDDPGTLDSDLPLVPFAQAFEEATESTMFR 241

Query: 278 FITPTFIWKAMRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLL 337
           F+ P FIWK +++ +IG EK L++++  V EF + ++  R  +L    E      RSD+L
Sbjct: 242 FMIPPFIWKPLKFFDIGYEKGLRKAVDVVHEFVDKMVVDRICKLK---EEGTLGNRSDVL 301

Query: 338 TVFMKLRD----EQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKIL 397
           +  +++      ++  P + KF R  C +FILAGRDTSSVAL+WFFW++ ++P+VE+KI+
Sbjct: 302 SRIIEIESHKTTDEKDPSTIKFFRQFCTSFILAGRDTSSVALTWFFWVIQKHPEVENKII 361

Query: 398 AEICRIVGEREEAKTRELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVED 457
            EI  I+ +R ++ T +   +  +F  +E+  M YL AA+SE +RLYP +P + K+ +ED
Sbjct: 362 REISEILRQRGDSPTSK---NESLFTVKELNDMVYLQAALSETMRLYPPIPMEMKQAIED 421

Query: 458 DTFPDGTILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNG 517
           D FPDGT ++KG ++ +A YAMGRME+IWGKDC  FKPERW++ G F+++  +++  FN 
Sbjct: 422 DVFPDGTFIRKGSRVYFATYAMGRMESIWGKDCESFKPERWIQSGNFVNDDQFKYVVFNA 481

Query: 518 GPRLCLGKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNL 566
           GPRLCLGK FAY QMK +AAS++SRY + V   H V P+++ T YM+ GLKV +
Sbjct: 482 GPRLCLGKTFAYLQMKTIAASVLSRYSIKVAKDHVVVPRVTTTLYMRHGLKVTI 521

BLAST of IVF0003161 vs. TAIR 10
Match: AT3G26125.1 (cytochrome P450, family 86, subfamily C, polypeptide 2 )

HSP 1 Score: 463.0 bits (1190), Expect = 3.5e-130
Identity = 230/531 (43.31%), Postives = 339/531 (63.84%), Query Frame = 0

Query: 47  SISVRHFGLQSLLDVQILEISFALFVFLVIHVVRQKRRHGLPI-WPIVGMLPALITATRG 106
           S+  R  G   L DV I  +   LF  L   +  +    G P+ WP+ G+ P        
Sbjct: 9   SLIDRISGHLCLQDVSIALLGLFLFGCLQAKLTNK----GEPVQWPVFGITPEFFLHVH- 68

Query: 107 NLYEWITDLLCRHKGTFQFRGPWLTSLNCVVTADPRNLEHLLKTKFSSYPKGSFFRDTVR 166
           +++ W+T  L + +GTF ++G W +     +T+ P N+E++LKT F ++PKG F+++  R
Sbjct: 69  DMFGWVTRCLTKSRGTFPYQGIWFSGSYGAMTSVPANIEYMLKTNFKNFPKGEFYKERFR 128

Query: 167 DLLGDGIFGADDETWQRQRKVASIEFHSARFRQMTVESLVELVHSRLEAVLEDFAGKSTT 226
           DLL DGIF ADDE+W+ QR++   E HS RF   + ++  +L+  +L  V+E F+     
Sbjct: 129 DLLEDGIFNADDESWKEQRRIIITEMHSTRFVDHSFQTTRDLIERKLLKVMESFSKSQEA 188

Query: 227 VDLQDVLLRLTFDNVCMIAFGVDPGCLRGGLPEIPFAKAFEDATEVTVMRFITPTFIWKA 286
            DLQ++LLRLTFDN+C+   G DPG L   LP +PFAKAFE+ATE T+ RF+ P F+WK 
Sbjct: 189 FDLQEILLRLTFDNICIAGLGDDPGTLDDDLPHVPFAKAFEEATESTLFRFMIPPFVWKP 248

Query: 287 MRYLNIGIEKKLKQSIKAVDEFAENVIQTRKRELSTPAESQADNKRSDLLTVFMKL---- 346
           M++ +IG EK L+++++ V  F + ++  R   L    +    N +SD+L+  +++    
Sbjct: 249 MKFFDIGYEKGLREAVETVHNFIDKMVVERIAMLKD--QGTLANSKSDVLSRLIQIESHK 308

Query: 347 RDEQGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPDVEDKILAEICRIVGER 406
           R ++   F+ KF R  C +FILAGRDTSSVAL+WFFWL+ ++P+VE KIL EI  I+ +R
Sbjct: 309 RGDENDRFTAKFFRQFCTSFILAGRDTSSVALTWFFWLITKHPEVETKILHEIREILNQR 368

Query: 407 EEAKTR----ELRFDSLVFKPEEIKKMEYLHAAISEALRLYPSVPFDHKEVVEDDTFPDG 466
           E+ K +      +  S  F  +E+  M YL AA+SE+LRLYP +P + K+  E+D FPDG
Sbjct: 369 EKNKYKLDETGEKESSRHFTVKELNDMVYLQAALSESLRLYPPIPMEMKQATEEDVFPDG 428

Query: 467 TILKKGMKIIYAIYAMGRMEAIWGKDCREFKPERWMRDGRFMSESVYRFTAFNGGPRLCL 526
           T L+KG ++ +++YAMGRME+IWGKDC  FKPERW++ G+++S+  +++  FN GPRLCL
Sbjct: 429 TFLRKGSRVYFSVYAMGRMESIWGKDCEMFKPERWIQGGQYVSDDQFKYVVFNAGPRLCL 488

Query: 527 GKDFAYYQMKYVAASIISRYRVAVVAGHPVEPKLSLTFYMKKGLKVNLIKR 569
           GK FAY QMK VAASI+  Y + V   H V P+++ T YMK GLKV +  R
Sbjct: 489 GKTFAYLQMKMVAASILLNYSIKVDQDHVVVPRVTTTLYMKHGLKVRITPR 532

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMY15.0e-22270.07Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1[more]
Q9CAD66.5e-12946.81Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1[more]
B3RFJ62.7e-12749.69Cytochrome P450 86A22 OS=Petunia hybrida OX=4102 GN=CYP86A22 PE=1 SV=1[more]
Q9LMM14.7e-12748.58Cytochrome P450 86A4 OS=Arabidopsis thaliana OX=3702 GN=CYP86A4 PE=1 SV=1[more]
O230662.0e-12547.25Cytochrome P450 86A2 OS=Arabidopsis thaliana OX=3702 GN=CYP86A2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A1S3CEY80.0e+0099.66LOW QUALITY PROTEIN: cytochrome P450 86B1-like OS=Cucumis melo OX=3656 GN=LOC103... [more]
A0A5A7UTT40.0e+00100.00Cytochrome P450 86B1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A0A0K8792.7e-30394.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G420770 PE=3 SV=1[more]
A0A6J1GQ361.6e-29589.54cytochrome P450 86B1-like OS=Cucurbita moschata OX=3662 GN=LOC111456469 PE=3 SV=... [more]
A0A6J1JMG11.8e-29489.36cytochrome P450 86B1-like OS=Cucurbita maxima OX=3661 GN=LOC111488206 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_008461361.10.099.66PREDICTED: LOW QUALITY PROTEIN: cytochrome P450 86B1-like [Cucumis melo][more]
XP_004135954.30.094.84cytochrome P450 86B1 [Cucumis sativus] >KAE8646384.1 hypothetical protein Csa_01... [more]
KAA0058578.10.0100.00cytochrome P450 86B1-like [Cucumis melo var. makuwa] >TYK10378.1 cytochrome P450... [more]
XP_038899430.10.090.78cytochrome P450 86B1-like [Benincasa hispida][more]
KAG7014270.10.089.38Cytochrome P450 86B1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G23190.13.6e-22370.07cytochrome P450, family 86, subfamily B, polypeptide 1 [more]
AT5G08250.13.2e-20870.18Cytochrome P450 superfamily protein [more]
AT1G24540.11.3e-14046.62cytochrome P450, family 86, subfamily C, polypeptide 1 [more]
AT1G13140.15.8e-13344.38cytochrome P450, family 86, subfamily C, polypeptide 3 [more]
AT3G26125.13.5e-13043.31cytochrome P450, family 86, subfamily C, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 353..370
score: 28.15
coord: 428..446
score: 31.1
coord: 506..516
score: 42.0
coord: 516..539
score: 19.46
coord: 373..399
score: 33.21
IPR001128Cytochrome P450PRINTSPR00385P450coord: 507..516
score: 46.03
coord: 429..440
score: 44.94
coord: 516..527
score: 35.08
coord: 364..381
score: 38.3
IPR001128Cytochrome P450PFAMPF00067p450coord: 87..553
e-value: 6.0E-76
score: 256.0
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 82..578
e-value: 2.7E-105
score: 354.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 86..568
NoneNo IPR availablePANTHERPTHR24296:SF177CYTOCHROME P450 86B1-LIKEcoord: 51..569
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 51..569
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 509..518

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0003161.1IVF0003161.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen