IVF0003091 (gene) Melon (IVF77) v1

Overview
NameIVF0003091
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionSplicing factor 3B subunit 4
Locationtig00000656: 574797 .. 578547 (-)
RNA-Seq ExpressionIVF0003091
SyntenyIVF0003091
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCCATAGAAAATTGTTAGTTCTCGCGAGTGTGCAAAGGGGTTTTGGTCTGTTGTAATTTGTGAACTTCAAACGGAGGCAACTGCTTCACCGACTAGCCGGAGGAGAAGATGACGACGAAGATAGCGCCGGGAGTCGGAGCCAATCTGCTCGGCCAGCACGCCCCCGAGAGGAATCAAGACGCCACTGCCTATGTCGGAAATCTAGATCCCCAGGTTTTTTTTTTTCCCTTATTTCTCCTTTCTGAATCAGCTAATTCACCTTCAAAACCCTTCGTCTGGGGTTTCCCCGTTCTTCTAATTTCATTTTTATCATACCCTTTTACTGTTTTTTGTGCGCCCTGCCAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGTCCAGTTGGTGAGTTCGTTCATTTCTTTTTCCTAATGAACTCTTAGGGTTTTCTGAAAATTCTGTTGAAGTTATTTTTGTTCCCTTCTTATTCTTGGTTGGATTATTTCCTTAAACAAATTGTACCTACTTCTTGTTTTGTAGTTAATGTGTATGTTCCCAAGGATAGAGTCACTAATTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGCGAGGAAGATGCAGATTATGTATGTTATGTTGTGGACTCTTTTGCCTTTTTATCCTCATAGTTATATGCTTTCGGAAGTTATCTTGATTTTCCATTCTCAATGGTTTTGCAGGCAATCAAGGTGCTAAATATGATCAAGCTCTATGGGAAGCCAATTCGCGTAAACAAGGCATGTAGGAAACTTTTTCGTTCAACTGCACTATAATGTGGTCTGATGTACTGTTCAGCAAAGGAGGATGGTATTTTATCTTCTTATAAAAATGGATATTTAACTTTTATTATGTGCATTGCATATTCCAGGCGTCTCAAGACAAAAAGAGTTTGGATGTGGGGGCAAATCTCTTCATAGGAAACCTAGACCCGGTAAGTCTTTATTTTATTATGATTATATCTGGAAAACTTGCAAGACTGATGTTTTAAAGATTGAAACCAAACCATGGTGTTTCTTTCAGGATGTTGATGAGAAGCTTCTCTACGATACTTTCAGTGCATTTGGGGTCATCGTGACGAATCCTAAGGTTATTACTTTTTCCATAAACCCATCACTCTCATATGTTTTATTTATCATAGGAGATTAGAGTTCATTTTCGAGCAAACTACTCACTAGTAAATTATAGTTCCTTTTCAAGTCAACTACTTACTCTTTAAAGAAGTGATTTAATGACAATTTAACGTAGTGAAGGCCTTTCATGGAAACATTAAACAATCACAATTGCTTTTTAGTATGTTTTACCCGTATACAATGTTTACGGTTGTTTATTTTTTCTGGAATATGCTGAACATCATTGGTCTCTATTCTGTATTTTCTTGATGGGAGTTGTAAGTAAACTTTTAGGAAGTTTGTGTTATTTGTGGTTTTTATTAATGAATTTCTCTTTAGTTTGCAATGAGAAAATTCATTTCCTATACGATATTGCCTCTTTGGCCAGAAAGTCAATCTAGATTATGAAATAGTATAACATTCCTTTTCTTATTCTTTTTGTTTTATCCCTCTCTCCTTTAGTTGTAGTGTATTGACATGTCTCTTCTATTTCATGTTTCAATCATAATGGTGGTTGAACAGCCAGTTTATGATTTCTTTTGTCTGTATTCATTGTCTTTTTTAATAATATATAATTTCTATCACTTCCTTAACTGTTTTCTTGTCTTACCATTACTTAGAATTTCTTTTTACTCCAAGGATTTTTTAGCTGGGTTTTCTTCCACGTGGGTTACTCCACCTCTCTATACTATCATGTTCTTTATGGAAATGGGACTTGTTATATGACCTTACTTTATATTTACTGTTACTCTCCTGAAGTGTGTAGTTGGGTACGTTGGATCAGGAATTTGTTAGGACTTTAAGGGGTTAATAACATCATGGCCGTTGGAATGCTTAGACTTCTTGGCTACTGAATTTTGCTATCAAATCTGTTTCCTACCATGTTTCAAGAGCAATATGAACCAAAGTTTTGAACTTTTGAGTGCATAAGGCACCACATTATTCCCTTTACCATCCTTTTAAATAAAAGATCCCTTCTATTTAGCATGTGGAATACAAAATTTCAGTCTGAAGTGGGCACTTGAAAAGTGTTTATATTTTCTTTGTATTAAGCTATTTACCAATGTTTCAATATTCTTTTGCTTGGTTCTTTTGTTGTTTCTCCTTTGTGATCGTTTGTAGATCGTTTTCACATTTGTATTGTCCGAGTTCGTCTTTGTTTAGAGCATATTATCCTTTTCTTATTTTTCTCTTAGTATAATACTCTTGTACTTTAAGCATTAGTCTCATTTATTATTAATAAAGAGTTTCGTCTTTGTTTCAAAAAATGAAATAAAAGGCCCCATCTTGTTGTATGCCCTATTTATCCTCTTTGGTTCTTTCTCATCTGGTGTGTACTTGCTACTGAGCTCTTTTTTGGGATGTGTTAGAACTGTTTTGCATAGATCTGAGTCTTAATACATTTATTTTCCGACTTGAAATGTCTTTCTTTTTTTATTAACCAGATTATGAGGGATCCAGATACAGGAAATTCGCGTGGGTTTGGTTTTATTAGCTATGACTCCTTTGAGGCATCTGATGCAGCAATTGAGGTGTGTTATTGCTTTGTTTATCGGCAATCATTTCAATCCCGCAACATTTGCTGTCTTTATATATTATATATGAAGTTTCAAATGCCAACAAGATTTAAAATAATTTGTCCACTTGGTAACTCAAACCTCGAAAAAGAATGAAGTAACCTTATCTTGACACAATTTTTTGCTACTTTGCAATAGGCAATGAATGGTCAGTATCTCTGTAATCGTCAAATAACAGTTTCGTATGCCTATAAGAAAGATACCAAGGGAGAGCGGCACGGCACACCTGCAGGTAAGAGTTGTTGCTGATCTTTGAAAATTTGTGAATTTTTTTTAGGTCAGGAACAGGATTCAAATTTTTGGCGACTGAGATGAGAAGTTTATCATCATTGATTAATTTACCTCTGGTGTTATCTTTTTCTATGTGTAGAGAGAGTATTGGCTGCTAGCAATCCCCAAAAGAGTAGGCCTCACACTTTGTTCGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAATGGTGCGATTGGAGCTCCGGTGCCACCGCGCCCCTTTGCTAACGGTGCAATCACTCCCAACCCCATTCCTGCAATACGTCCTTCGCCACCACAAGGGGTGGCTTTCCCCCCTATGCCAATGGCCGGACAACCACCAGCCTGGCAAGGTCAGCCGCAGCAACCAGGTCAAATGATGCCGGGTTCCATGATTCCACCACCAGTTCAGCAGTTCCGGCCACCTCCCCAAACATGCCAATGCCACCACCGCAGGCTCAAGCACATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGCACAGCCACCGCTTTGGCGACCACCGCCACCGCCAATGCAGCAGCAGCAGCCTGGAAGGCCACCTATGCAAATGTCATCAATGCCCCCGCCGCCACCTCCAAATCATATTCCACCACCTCTACCTTCTTCTGGCTGATACATCAATATATTGCTAGCCATGAAAATGAGATCATGTTTATTTCCTTTTTGTGATAATATTCAGTAGGCTGTCATATTGTCATACAAATTGTTAACACTTCAATCCGAGAAATTAGCAGGAGGAAATTCGGC

mRNA sequence

CCCCATAGAAAATTGTTAGTTCTCGCGAGTGTGCAAAGGGGTTTTGGTCTGTTGTAATTTGTGAACTTCAAACGGAGGCAACTGCTTCACCGACTAGCCGGAGGAGAAGATGACGACGAAGATAGCGCCGGGAGTCGGAGCCAATCTGCTCGGCCAGCACGCCCCCGAGAGGAATCAAGACGCCACTGCCTATGTCGGAAATCTAGATCCCCAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGTCCAGTTGTTAATGTGTATGTTCCCAAGGATAGAGTCACTAATTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGCGAGGAAGATGCAGATTATGCAATCAAGGTGCTAAATATGATCAAGCTCTATGGGAAGCCAATTCGCGTAAACAAGGCGTCTCAAGACAAAAAGAGTTTGGATGTGGGGGCAAATCTCTTCATAGGAAACCTAGACCCGGATGTTGATGAGAAGCTTCTCTACGATACTTTCAGTGCATTTGGGGTCATCGTGACGAATCCTAAGATTATGAGGGATCCAGATACAGGAAATTCGCGTGGGTTTGGTTTTATTAGCTATGACTCCTTTGAGGCATCTGATGCAGCAATTGAGGCAATGAATGGTCAGTATCTCTGTAATCGTCAAATAACAGTTTCGTATGCCTATAAGAAAGATACCAAGGGAGAGCGGCACGGCACACCTGCAGAGAGAGTATTGGCTGCTAGCAATCCCCAAAAGAGTAGGCCTCACACTTTGTTCGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAATGGTGCGATTGGAGCTCCGGTGCCACCGCGCCCCTTTGCTAACGGTGCAATCACTCCCAACCCCATTCCTGCAATACGTCCTTCGCCACCACAAGGGGTGGCTTTCCCCCCTATGCCAATGGCCGGACAACCACCAGCCTGGCAAGGTCAGCCGCAGCAACCAGGTCAAATGATGCCGGGTTCCATGATTCCACCACCAGTTCAGCAGTTCCGGCCACCTCCCCAAACATGCCAATGCCACCACCGCAGGCTCAAGCACATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGCACAGCCACCGCTTTGGCGACCACCGCCACCGCCAATGCAGCAGCAGCAGCCTGGAAGGCCACCTATGCAAATGTCATCAATGCCCCCGCCGCCACCTCCAAATCATATTCCACCACCTCTACCTTCTTCTGGCTGATACATCAATATATTGCTAGCCATGAAAATGAGATCATGTTTATTTCCTTTTTGTGATAATATTCAGTAGGCTGTCATATTGTCATACAAATTGTTAACACTTCAATCCGAGAAATTAGCAGGAGGAAATTCGGC

Coding sequence (CDS)

ATGACGACGAAGATAGCGCCGGGAGTCGGAGCCAATCTGCTCGGCCAGCACGCCCCCGAGAGGAATCAAGACGCCACTGCCTATGTCGGAAATCTAGATCCCCAGGTTTCTGAGGAGTTGCTTTGGGAGCTGTTTGTTCAAGCCGGTCCAGTTGTTAATGTGTATGTTCCCAAGGATAGAGTCACTAATTTGCATCAAGGATATGGATTCATTGAGTTTCGCAGCGAGGAAGATGCAGATTATGCAATCAAGGTGCTAAATATGATCAAGCTCTATGGGAAGCCAATTCGCGTAAACAAGGCGTCTCAAGACAAAAAGAGTTTGGATGTGGGGGCAAATCTCTTCATAGGAAACCTAGACCCGGATGTTGATGAGAAGCTTCTCTACGATACTTTCAGTGCATTTGGGGTCATCGTGACGAATCCTAAGATTATGAGGGATCCAGATACAGGAAATTCGCGTGGGTTTGGTTTTATTAGCTATGACTCCTTTGAGGCATCTGATGCAGCAATTGAGGCAATGAATGGTCAGTATCTCTGTAATCGTCAAATAACAGTTTCGTATGCCTATAAGAAAGATACCAAGGGAGAGCGGCACGGCACACCTGCAGAGAGAGTATTGGCTGCTAGCAATCCCCAAAAGAGTAGGCCTCACACTTTGTTCGCAAGTGGCCCTCCGACACTTCCAAAGGTTGCACAGCCTAATGGTGCGATTGGAGCTCCGGTGCCACCGCGCCCCTTTGCTAACGGTGCAATCACTCCCAACCCCATTCCTGCAATACGTCCTTCGCCACCACAAGGGGTGGCTTTCCCCCCTATGCCAATGGCCGGACAACCACCAGCCTGGCAAGGTCAGCCGCAGCAACCAGGTCAAATGATGCCGGGTTCCATGATTCCACCACCAGTTCAGCAGTTCCGGCCACCTCCCCAAACATGCCAATGCCACCACCGCAGGCTCAAGCACATTCCATGCCTATCCCTCCACCAATGGGAATGGGAGGAGCACAGCCACCGCTTTGGCGACCACCGCCACCGCCAATGCAGCAGCAGCAGCCTGGAAGGCCACCTATGCAAATGTCATCAATGCCCCCGCCGCCACCTCCAAATCATATTCCACCACCTCTACCTTCTTCTGGCTGATACATCAATATATTGCTAG

Protein sequence

MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGAPVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPPPVQQFRPPPQTCQCHHRRLKHIPCLSLHQWEWEEHSHRFGDHRHRQCSSSSLEGHLCKCHQCPRRHLQIIFHHLYLLLADTSIYC
Homology
BLAST of IVF0003091 vs. ExPASy Swiss-Prot
Match: Q15427 (Splicing factor 3B subunit 4 OS=Homo sapiens OX=9606 GN=SF3B4 PE=1 SV=1)

HSP 1 Score: 347.4 bits (890), Expect = 2.0e-94
Identity = 193/309 (62.46%), Postives = 219/309 (70.87%), Query Frame = 0

Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPP---------------TLPKVAQPNGAIGAPV 259
           G+ AER+LAA NP  Q  RPH LFA  PP                 P    P G+   PV
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPV 247

Query: 260 PPRPFANGAITPNPIPAIRPSP-PQGVAFPPMPMAGQPPAWQGQP----QQPGQMMPGSM 307
           PP     GA+ P   PA+ P P P G A    P AG P A  G P      P    PG M
Sbjct: 248 PP----PGALPPGIPPAMPPPPMPPGAAGHGPPSAGTPGA--GHPGHGHSHPHPFPPGGM 307

BLAST of IVF0003091 vs. ExPASy Swiss-Prot
Match: Q8QZY9 (Splicing factor 3B subunit 4 OS=Mus musculus OX=10090 GN=Sf3b4 PE=1 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.9e-94
Identity = 192/309 (62.14%), Postives = 218/309 (70.55%), Query Frame = 0

Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPP---------------TLPKVAQPNGAIGAPV 259
           G+ AER+LAA NP  Q  RPH LFA  PP                 P    P G+   PV
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPV 247

Query: 260 PPRPFANGAITPNPIPAIRPSP-PQGVAFPPMPMAGQPPAWQGQP----QQPGQMMPGSM 307
           PP     GA+ P   PA+ P P P G      P AG P A  G P      P    PG M
Sbjct: 248 PP----PGALPPGIPPAMPPPPMPPGAGGHGPPAAGTPGA--GHPGHGHSHPHPFPPGGM 307

BLAST of IVF0003091 vs. ExPASy Swiss-Prot
Match: Q6AYL5 (Splicing factor 3B subunit 4 OS=Rattus norvegicus OX=10116 GN=Sf3b4 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.9e-94
Identity = 192/309 (62.14%), Postives = 218/309 (70.55%), Query Frame = 0

Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE LLWELF+QAGPVVN ++PKDRVT  HQGYGF+EF SEEDA
Sbjct: 8   ERNQDATVYVGGLDEKVSEPLLWELFLQAGPVVNTHMPKDRVTGQHQGYGFVEFLSEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK++NMIKLYGKPIRVNKAS   K+LDVGAN+FIGNLDP++DEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKIMNMIKLYGKPIRVNKASAHNKNLDVGANIFIGNLDPEIDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRDPDTGNS+G+ FI++ SF+ASDAAIEAMNGQYLCNR ITVSYA+KKD+KGERH
Sbjct: 128 QTPKIMRDPDTGNSKGYAFINFASFDASDAAIEAMNGQYLCNRPITVSYAFKKDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFASGPP---------------TLPKVAQPNGAIGAPV 259
           G+ AER+LAA NP  Q  RPH LFA  PP                 P    P G+   PV
Sbjct: 188 GSAAERLLAAQNPLSQADRPHQLFADAPPPPSAPNPVVSSLGSGLPPPGMPPPGSFPPPV 247

Query: 260 PPRPFANGAITPNPIPAIRPSP-PQGVAFPPMPMAGQPPAWQGQP----QQPGQMMPGSM 307
           PP     GA+ P   PA+ P P P G      P AG P A  G P      P    PG M
Sbjct: 248 PP----PGALPPGIPPAMPPPPMPPGAGGHGPPAAGTPGA--GHPGHGHSHPHPFPPGGM 307

BLAST of IVF0003091 vs. ExPASy Swiss-Prot
Match: Q09442 (Splicing factor 3B subunit 4 OS=Caenorhabditis elegans OX=6239 GN=sap-49 PE=1 SV=2)

HSP 1 Score: 324.7 bits (831), Expect = 1.4e-87
Identity = 187/327 (57.19%), Postives = 222/327 (67.89%), Query Frame = 0

Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           ERNQDAT YVG LD +VSE +LWEL VQAGPVV+V +PKDRVT  HQG+GF+EF  EEDA
Sbjct: 8   ERNQDATIYVGGLDEKVSESILWELMVQAGPVVSVNMPKDRVTANHQGFGFVEFMGEEDA 67

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVIV 139
           DYAIK+LNMIKLYGKPI+VNKAS  +K++DVGAN+F+GNLDP+VDEKLLYDTFSAFGVI+
Sbjct: 68  DYAIKILNMIKLYGKPIKVNKASAHEKNMDVGANIFVGNLDPEVDEKLLYDTFSAFGVIL 127

Query: 140 TNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGERH 199
             PKIMRD D+G S+GF FI++ SFEASD A+EAMNGQ+LCNR ITVSYA+K+D+KGERH
Sbjct: 128 QVPKIMRDVDSGTSKGFAFINFASFEASDTALEAMNGQFLCNRAITVSYAFKRDSKGERH 187

Query: 200 GTPAERVLAASNP--QKSRPHTLFAS---GPPTLPKVAQP--NGAIGA------------ 259
           GT AER+LAA NP   K RPH +F+    G P    +A P  + AI A            
Sbjct: 188 GTAAERMLAAQNPLFPKDRPHQVFSDVPLGVPANTPLAMPGVHAAIAAHATGRPGYQPPP 247

Query: 260 --------------PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQ-----PPA 309
                         PVPP P    ++TP P P + P+P      PP P +G      PP 
Sbjct: 248 LMGMAQSGYQGQYPPVPPPP---PSVTPMP-PPMPPTPGMTPRPPPPPSSGMWPPPPPPP 307

BLAST of IVF0003091 vs. ExPASy Swiss-Prot
Match: O14102 (Spliceosome-associated protein 49 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sap49 PE=3 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 6.8e-66
Identity = 144/298 (48.32%), Postives = 193/298 (64.77%), Query Frame = 0

Query: 20  ERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDA 79
           +RNQDAT Y+GNLD +V++ +L+EL +QAGPVVN+++P+DRV N H G+GF EF  E+D 
Sbjct: 6   DRNQDATIYLGNLDEKVTDSILFELCLQAGPVVNIHIPRDRVRNSHNGFGFCEFLHEQDV 65

Query: 80  DYAIKVLNMIKLYGKPIRVNKASQDK-KSLDVGANLFIGNLDPDVDEKLLYDTFSAFGVI 139
           +YA ++LN +KL+GKPIRVN+ASQD+  +  +GANLF+GNLDP VDE++LYDTFSA G +
Sbjct: 66  EYACQILNQVKLFGKPIRVNRASQDRGVNTLIGANLFVGNLDPLVDERVLYDTFSALGQL 125

Query: 140 VTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKGER 199
           V  P++ RD + G S+G+GF+SYDSFE +DAAIEAMN Q+L N+ ITVSYA+K++ KGER
Sbjct: 126 VKAPQVARD-ENGRSKGYGFVSYDSFETADAAIEAMNNQFLMNKPITVSYAFKREGKGER 185

Query: 200 HGTPAERVLAASNPQKSRPHTLFASGPPTL-PKVAQPNGAIGAP-------VPPRP---- 259
           HG  AER LAA+  +     T  ++ PP   P    P  A   P       +PP P    
Sbjct: 186 HGDIAERKLAAAAKKNKVAVTPQSTLPPGFSPATPAPTSAANTPATIAATSIPPVPNVPL 245

Query: 260 -FANGAITPNPIPAIRP--SPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPPP 302
             A  A+ P  IP + P  +       P MPM   P    G P          ++PPP
Sbjct: 246 VGATTAVPPLSIPNVLPFTAAQHFPGMPAMPMMNVPMGPGGAP----------LVPPP 292

BLAST of IVF0003091 vs. ExPASy TrEMBL
Match: A0A5D3DJ06 (Splicing factor 3B subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00160 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.1e-175
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 310
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. ExPASy TrEMBL
Match: A0A0A0LAZ0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G172960 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.1e-175
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 310
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. ExPASy TrEMBL
Match: A0A1S3AYG3 (splicing factor 3B subunit 4 OS=Cucumis melo OX=3656 GN=LOC103483958 PE=3 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 1.6e-174
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPG MMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGHMMPGSMIPP 300

Query: 301 PVQQFRPPP 310
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. ExPASy TrEMBL
Match: A0A5A7VR80 (Splicing factor 3B subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00160 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.2e-171
Identity = 306/309 (99.03%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKA   KKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKAY--KKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 310
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 307

BLAST of IVF0003091 vs. ExPASy TrEMBL
Match: A0A6J1C8Z7 (splicing factor 3B subunit 4 OS=Momordica charantia OX=3673 GN=LOC111009481 PE=3 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 5.8e-169
Identity = 301/309 (97.41%), Postives = 302/309 (97.73%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNG IGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGVIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANG I P+PIPAIRP PPQGVAFPPM M GQ PAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGTIAPSPIPAIRPPPPQGVAFPPMQMPGQ-PAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 310
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 308

BLAST of IVF0003091 vs. NCBI nr
Match: TYK23651.1 (splicing factor 3B subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 623 bits (1607), Expect = 8.78e-223
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 309
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. NCBI nr
Match: XP_004147654.1 (splicing factor 3B subunit 4 [Cucumis sativus] >KGN57241.1 hypothetical protein Csa_009897 [Cucumis sativus])

HSP 1 Score: 623 bits (1607), Expect = 8.78e-223
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 309
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. NCBI nr
Match: XP_008439058.1 (PREDICTED: splicing factor 3B subunit 4 [Cucumis melo])

HSP 1 Score: 621 bits (1602), Expect = 5.07e-222
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPG MMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGHMMPGSMIPP 300

Query: 301 PVQQFRPPP 309
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. NCBI nr
Match: XP_038878136.1 (splicing factor 3B subunit 4 [Benincasa hispida])

HSP 1 Score: 618 bits (1593), Expect = 1.15e-220
Identity = 306/309 (99.03%), Postives = 308/309 (99.68%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITP+PIPAIRP PPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGS+IPP
Sbjct: 241 PVPPRPFANGAITPSPIPAIRPPPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSIIPP 300

Query: 301 PVQQFRPPP 309
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 309

BLAST of IVF0003091 vs. NCBI nr
Match: KAA0067649.1 (splicing factor 3B subunit 4 [Cucumis melo var. makuwa])

HSP 1 Score: 612 bits (1577), Expect = 3.02e-218
Identity = 306/309 (99.03%), Postives = 306/309 (99.03%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKA   KKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKAY--KKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC
Sbjct: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240
           NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNPQKSRPHTLFASGPPTLPKVAQPNGAIGA 240

Query: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300
           PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP
Sbjct: 241 PVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMIPP 300

Query: 301 PVQQFRPPP 309
           PVQQFRPPP
Sbjct: 301 PVQQFRPPP 307

BLAST of IVF0003091 vs. TAIR 10
Match: AT2G18510.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 465.3 bits (1196), Expect = 4.7e-131
Identity = 246/310 (79.35%), Postives = 261/310 (84.19%), Query Frame = 0

Query: 1   MTTKIAPGVGANLLGQHAPERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDR 60
           MTT+IAPGVGANLLGQH+ ERNQDAT YVG LD Q+SEELLWELFVQAGPVVNVYVPKDR
Sbjct: 1   MTTRIAPGVGANLLGQHSAERNQDATVYVGGLDAQLSEELLWELFVQAGPVVNVYVPKDR 60

Query: 61  VTNLHQGYGFIEFRSEEDADYAIKVLNMIKLYGKPIRVNKASQDKKSLDVGANLFIGNLD 120
           VTNLHQ YGFIE+RSEEDADYAIKVLNMIKL+GKPIRVNKASQDKKSLDVGANLFIGNLD
Sbjct: 61  VTNLHQNYGFIEYRSEEDADYAIKVLNMIKLHGKPIRVNKASQDKKSLDVGANLFIGNLD 120

Query: 121 PDVDEKLLYDTFSAFGVIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLC 180
           PDVDEKLLYDTFSAFGVI +NPKIMRDPDTGNSRGFGFISYDSFEASDAAIE+M GQYL 
Sbjct: 121 PDVDEKLLYDTFSAFGVIASNPKIMRDPDTGNSRGFGFISYDSFEASDAAIESMTGQYLS 180

Query: 181 NRQITVSYAYKKDTKGERHGTPAERVLAASNP--QKSRPHTLFASGPPTLPKVAQPNGAI 240
           NRQITVSYAYKKDTKGERHGTPAER+LAA+NP  QKSRPHTLFA GPP+     Q NG  
Sbjct: 181 NRQITVSYAYKKDTKGERHGTPAERLLAATNPTAQKSRPHTLFAMGPPS--SAPQVNGL- 240

Query: 241 GAPVPPRPFANGAITPNPIPAIRPSPPQGVAFPPMPMAGQPPAWQGQPQQPGQMMPGSMI 300
                PRPFANG++ P PIPA R  PP     PP     QPP+W  QPQQ       SM+
Sbjct: 241 -----PRPFANGSMQPVPIPAPRQPPPP----PPQVYQTQPPSWPSQPQQ------HSMV 291

Query: 301 PPPVQQFRPP 309
           PPP+ QFRPP
Sbjct: 301 PPPM-QFRPP 291

BLAST of IVF0003091 vs. TAIR 10
Match: AT4G34110.1 (poly(A) binding protein 2 )

HSP 1 Score: 131.7 bits (330), Expect = 1.2e-30
Identity = 73/164 (44.51%), Postives = 105/164 (64.02%), Query Frame = 0

Query: 24  DATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAI 83
           + + YVG+LD  V++  L++ F Q G VV V V +D VT    GYG++ F + +DA  AI
Sbjct: 35  NTSLYVGDLDFNVTDSQLFDAFGQMGTVVTVRVCRDLVTRRSLGYGYVNFTNPQDAARAI 94

Query: 84  KVLNMIKLYGKPIRVNKASQDKKSLDVGA-NLFIGNLDPDVDEKLLYDTFSAFGVIVTNP 143
           + LN I LYGKPIRV  + +D      GA N+FI NLD  +D K L+DTFS+FG IV + 
Sbjct: 95  QELNYIPLYGKPIRVMYSHRDPSVRRSGAGNIFIKNLDESIDHKALHDTFSSFGNIV-SC 154

Query: 144 KIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITV 187
           K+  D  +G S+G+GF+ Y + E++  AIE +NG  L ++Q+ V
Sbjct: 155 KVAVD-SSGQSKGYGFVQYANEESAQKAIEKLNGMLLNDKQVYV 196

BLAST of IVF0003091 vs. TAIR 10
Match: AT1G49760.1 (poly(A) binding protein 8 )

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-30
Identity = 71/175 (40.57%), Postives = 109/175 (62.29%), Query Frame = 0

Query: 28  YVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLN 87
           YVG+LD  V++  L+E F QAG VV+V V +D  T    GYG++ + + +DA  A+  LN
Sbjct: 48  YVGDLDATVTDSQLFEAFTQAGQVVSVRVCRDMTTRRSLGYGYVNYATPQDASRALNELN 107

Query: 88  MIKLYGKPIRVNKASQDKKSLDVG-ANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMR 147
            + L G+ IRV  + +D      G  N+FI NLD  +D K L++TFSAFG I+ + K+  
Sbjct: 108 FMALNGRAIRVMYSVRDPSLRKSGVGNIFIKNLDKSIDHKALHETFSAFGPIL-SCKVAV 167

Query: 148 DPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVS---YAYKKDTKGER 199
           DP +G S+G+GF+ YD+ EA+  AI+ +NG  L ++Q+ V    +  ++D  GE+
Sbjct: 168 DP-SGQSKGYGFVQYDTDEAAQGAIDKLNGMLLNDKQVYVGPFVHKLQRDPSGEK 220

BLAST of IVF0003091 vs. TAIR 10
Match: AT1G49760.2 (poly(A) binding protein 8 )

HSP 1 Score: 131.3 bits (329), Expect = 1.6e-30
Identity = 71/175 (40.57%), Postives = 109/175 (62.29%), Query Frame = 0

Query: 28  YVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEEDADYAIKVLN 87
           YVG+LD  V++  L+E F QAG VV+V V +D  T    GYG++ + + +DA  A+  LN
Sbjct: 48  YVGDLDATVTDSQLFEAFTQAGQVVSVRVCRDMTTRRSLGYGYVNYATPQDASRALNELN 107

Query: 88  MIKLYGKPIRVNKASQDKKSLDVG-ANLFIGNLDPDVDEKLLYDTFSAFGVIVTNPKIMR 147
            + L G+ IRV  + +D      G  N+FI NLD  +D K L++TFSAFG I+ + K+  
Sbjct: 108 FMALNGRAIRVMYSVRDPSLRKSGVGNIFIKNLDKSIDHKALHETFSAFGPIL-SCKVAV 167

Query: 148 DPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVS---YAYKKDTKGER 199
           DP +G S+G+GF+ YD+ EA+  AI+ +NG  L ++Q+ V    +  ++D  GE+
Sbjct: 168 DP-SGQSKGYGFVQYDTDEAAQGAIDKLNGMLLNDKQVYVGPFVHKLQRDPSGEK 220

BLAST of IVF0003091 vs. TAIR 10
Match: AT1G22760.1 (poly(A) binding protein 3 )

HSP 1 Score: 123.6 bits (309), Expect = 3.4e-28
Identity = 65/189 (34.39%), Postives = 114/189 (60.32%), Query Frame = 0

Query: 18  APERNQDATAYVGNLDPQVSEELLWELFVQAGPVVNVYVPKDRVTNLHQGYGFIEFRSEE 77
           A + + +++ Y G+LDP+V+E  L++LF     VV+V V +D+      GY +I F +  
Sbjct: 42  ADQTHPNSSLYAGDLDPKVTEAHLFDLFKHVANVVSVRVCRDQ-NRRSLGYAYINFSNPN 101

Query: 78  DADYAIKVLNMIKLYGKPIRVNKASQDKKS-LDVGANLFIGNLDPDVDEKLLYDTFSAFG 137
           DA  A++ LN   L+ +PIR+  +++D  + L    N+FI NLD  +D K L++TFS+FG
Sbjct: 102 DAYRAMEALNYTPLFDRPIRIMLSNRDPSTRLSGKGNIFIKNLDASIDNKALFETFSSFG 161

Query: 138 VIVTNPKIMRDPDTGNSRGFGFISYDSFEASDAAIEAMNGQYLCNRQITVSYAYKKDTKG 197
            I+ + K+  D  TG S+G+GF+ ++  E++ AAI+ +NG  + ++Q+ V +  ++  + 
Sbjct: 162 TIL-SCKVAMDV-TGRSKGYGFVQFEKEESAQAAIDKLNGMLMNDKQVFVGHFIRRQERA 221

Query: 198 ERHGTPAER 206
               TP  R
Sbjct: 222 RDENTPTPR 227

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q154272.0e-9462.46Splicing factor 3B subunit 4 OS=Homo sapiens OX=9606 GN=SF3B4 PE=1 SV=1[more]
Q8QZY95.9e-9462.14Splicing factor 3B subunit 4 OS=Mus musculus OX=10090 GN=Sf3b4 PE=1 SV=1[more]
Q6AYL55.9e-9462.14Splicing factor 3B subunit 4 OS=Rattus norvegicus OX=10116 GN=Sf3b4 PE=2 SV=1[more]
Q094421.4e-8757.19Splicing factor 3B subunit 4 OS=Caenorhabditis elegans OX=6239 GN=sap-49 PE=1 SV... [more]
O141026.8e-6648.32Spliceosome-associated protein 49 OS=Schizosaccharomyces pombe (strain 972 / ATC... [more]
Match NameE-valueIdentityDescription
A0A5D3DJ064.1e-175100.00Splicing factor 3B subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A0A0LAZ04.1e-175100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G172960 PE=3 SV=1[more]
A0A1S3AYG31.6e-17499.68splicing factor 3B subunit 4 OS=Cucumis melo OX=3656 GN=LOC103483958 PE=3 SV=1[more]
A0A5A7VR801.2e-17199.03Splicing factor 3B subunit 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A6J1C8Z75.8e-16997.41splicing factor 3B subunit 4 OS=Momordica charantia OX=3673 GN=LOC111009481 PE=3... [more]
Match NameE-valueIdentityDescription
TYK23651.18.78e-223100.00splicing factor 3B subunit 4 [Cucumis melo var. makuwa][more]
XP_004147654.18.78e-223100.00splicing factor 3B subunit 4 [Cucumis sativus] >KGN57241.1 hypothetical protein ... [more]
XP_008439058.15.07e-22299.68PREDICTED: splicing factor 3B subunit 4 [Cucumis melo][more]
XP_038878136.11.15e-22099.03splicing factor 3B subunit 4 [Benincasa hispida][more]
KAA0067649.13.02e-21899.03splicing factor 3B subunit 4 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT2G18510.14.7e-13179.35RNA-binding (RRM/RBD/RNP motifs) family protein [more]
AT4G34110.11.2e-3044.51poly(A) binding protein 2 [more]
AT1G49760.11.6e-3040.57poly(A) binding protein 8 [more]
AT1G49760.21.6e-3040.57poly(A) binding protein 8 [more]
AT1G22760.13.4e-2834.39poly(A) binding protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000504RNA recognition motif domainSMARTSM00360rrm1_1coord: 26..99
e-value: 2.2E-25
score: 100.3
coord: 113..187
e-value: 1.4E-24
score: 97.7
IPR000504RNA recognition motif domainPFAMPF00076RRM_1coord: 28..97
e-value: 2.0E-19
score: 69.2
coord: 114..184
e-value: 5.1E-20
score: 71.1
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 25..103
score: 18.563835
IPR000504RNA recognition motif domainPROSITEPS50102RRMcoord: 112..191
score: 17.267626
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 107..204
e-value: 4.0E-26
score: 93.6
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 17..106
e-value: 2.2E-26
score: 93.9
NoneNo IPR availablePANTHERPTHR15241:SF330SPLICING FACTOR 3B SUBUNIT 4coord: 1..326
NoneNo IPR availablePANTHERPTHR15241TRANSFORMER-2-RELATEDcoord: 1..326
IPR034159SF3B4, RNA recognition motif 2CDDcd12335RRM2_SF3B4coord: 111..193
e-value: 1.12563E-64
score: 198.673
IPR034158SF3B4, RNA recognition motif 1CDDcd12334RRM1_SF3B4coord: 28..100
e-value: 5.8266E-55
score: 173.549
IPR035979RNA-binding domain superfamilySUPERFAMILY54928RNA-binding domain, RBDcoord: 25..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0003091.1IVF0003091.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0048026 positive regulation of mRNA splicing, via spliceosome
biological_process GO:0010075 regulation of meristem growth
biological_process GO:0006357 regulation of transcription by RNA polymerase II
cellular_component GO:0005689 U12-type spliceosomal complex
molecular_function GO:0003729 mRNA binding
molecular_function GO:0070063 RNA polymerase binding
molecular_function GO:0003676 nucleic acid binding