IVF0002614 (gene) Melon (IVF77) v1

Overview
NameIVF0002614
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionDNA-directed RNA polymerases II and V subunit 8A-like
Locationchr07: 22794788 .. 22798018 (-)
RNA-Seq ExpressionIVF0002614
SyntenyIVF0002614
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTCCTTATTATTTTTAAAAAACCCTAATTAAATTTATTTTCCTAAGCGTGAGTCATCGCCTCGTACTCACCGAGTCACGGTGAACTTCCTTTTCAGTGAACGAGTCACGCCCCCCATTTCTTCCACGAAGTCCCTACGTTTGCCCTAACCCCTCGCCGCTCCTTGACGCCGTCGGTCAGTTTCAACCCTCTGTTTTTGTCCACTCTCTCTCTATATATATACATATATATCACACACGTAGCTTATCTTATGATATTGTTTGTGCTTCATTGGTTTTTGCTTTTATCGGGTTTTAGAGTGGCTTTGTAATCTGGTTGTTGATTTTTTTTTCTTCTTTCAATACAAAACAATGTCGCTGATGGAATGAAAACGATAATATAATGGTTGTTGAAGTTGGAGATTGGATTCTTATGGTTTTTTTTATGTTAGCACCCCAAGTTTTTGGCTGCCTCTAATTCAATGGATGAGTTTAGGTTTAAGTATGTTTATCAACAAGGCATTGTGTATCTCCAAATGCACTAGTCGAGTTGATCTTCATCCACTGCTTGACTACAACAAATGTCTTTTCACTCCATTTCTCTATTTGATGTGAATCTCCAACCTGGCAGTAACTCAGTTATGCTGAGTGCTTGTCACAGAGATGTCAATGCTATGATCGAGGAGTCCTTCCTCAATCCGCCTTTTGGGAGAAAAGCCGGTTTCTTTGGCTTGCCGGCGTGTGTGCAATGTTATGAGTCTTGTAGGATGAGAGGAACGGTAGGATTTTAGGGTTGTGGAGAGGGAGCTTTGGTCCCTTGTTCATTTTCATGTTTCTCTTTGGGCTTCAACTTCAAAGACTTTTTTGTAATTGTTCTATAGGAATGATTTTGCATAGTTCGAACCCCTTCTTGTAAAGGGTACCTGCCTTTTGTGGACTTGGTTTTTTGTACGGCCGTATATTCTTTCATTTTTTCCTCGCTGAAAGTGGTCATTTTCATTTAAAAACTCAGTGGGTTGCCTCCCCATTTACACCGAATGTTTAACAAAGTTCTTATTTTCCAATCTTGTGCTTTGAAACCCAATGCATCCAGTCAATAATGCCGTTTTTTTGGCTAATCTGGTTCTGTTATCTGATGGTCATACATTTTCTAGCACTGCTGGTTAATTTCTATTATTGCCCTGTTAATTTACAGGTTAGCACTAACTTTTGGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTATTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGGTACATTTTTGTATAGTAGTCACTTTCATTTCACTTATACAATATTTCCTGGAAAGCTTGTTAGCAGACTACGGCAGTTTGGTTGTTATATATCTGTAAAATCATTGTGCCATTCTTTAACTTTCTGATGGCGGTTGGAGATGTACTTCGAGGCTTTAAATCTTATCCATTCCTTAAGAGGTTTCCTTTCAATCAGTTAGGATATTATGTGGAATATTACAAATTTTGTATTATAACTCTATGTAAAATATGTTTCAAAATAATTTTTCAACATATTTAGGCAGATGACGTTTATGTTTTCACAATCTGTCCAACAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATGTAAATTCAGAGATATACCCACTGAAAGAAGGCGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGTAACTTGTTCTTCCATTGATTTTAATCTGTTGTACACTGAACTTAATGTGGTGGTATTGGTTTGAAAATGATGTCCTGGTAGATTGAAGGATTTAGGCCCTGTTTCAATTCCACATCTAAATTTCTTGCTGTGTTTTAAAAGCGAAGCCAAATTTTGAAAACATAGAAAAGTAGTTTTTAAAAACTAAGAAATTGAGAGGAAAAGTTTAATTTTAAAACCCAAAAGCAAAAAATAGAATGGTTACCAAATGGAGTCCGAGTTATTGTTTTTTTGTTTTTGAAAATGTTATAAACATCCCTTTGTCCTCTAAAAAATTTGTTATCTACGTTAAAACCAAGCCTAAATTTGAGAACTAAAAAATGTAGTTTTAAAAAACTTGTTTTACTTTTTAGGATTTGATTAAGACTTCAATTCTTAAGAAAAATAGAAACCATTGGAAGAAATTGAGAGGAAGTAAGCTTAATCTGAAGAAACAAAAACCAATGGTTACCAAATGGGATCTTATTAGGCCATTTTGTCATGAACTACTAGTTTTGACATATCACTTTGAATATTAATAGGTAACAAGAAACTTTCAATCTGCTCAGAAATATATGCTTTCCATAGTTATTGCATATGTCAAGACAGGTCTTCATTGTTTTCTGACAACTATATTTGTTCTTTCTTCTGTTCAAACACTGATTTGGTCTTGAGCATTCTCTACACTTGAATATGTCTACTTAACAATCGTTAGGCTGCATAACAGGGCAATCGGAAGTCGCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGTGGAAAAGCGTATGTGCTATAACTTCTCGAAACAATTTTGATGTGACACTTTAATTTTACTTATGAGCATTAGAGAATCGAGAGCTTGACATATATATTCTATTAGAAGTATTATCTAAAAAAATTATATTTGATCTGAATCTAGTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGATCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGACTTTCATTTGATTTGAACTCTAATGATTTGCTACAAAAGCTCTGGGCATTGCATATCGCTATCAAATCTGTGAATGTTTAGTAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGATTGGGCAATGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTAGCAGAACCTTTCAAGTTCTCGAAAACGGAGTTTGAGCATGGTATGTATAGTTTGTAGTGTTGTGCGGCCGTACCTGAACGGTTTTGGCAAGCTTGATTTCTTTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATGACAGATATTTAAGAGGGTATGAGATTTGAACTTCTCACC

mRNA sequence

TTTCCTTATTATTTTTAAAAAACCCTAATTAAATTTATTTTCCTAAGCGTGAGTCATCGCCTCGTACTCACCGAGTCACGGTGAACTTCCTTTTCAGTGAACGAGTCACGCCCCCCATTTCTTCCACGAAGTCCCTACGTTTGCCCTAACCCCTCGCCGCTCCTTGACGCCGTCGGTTAGCACTAACTTTTGGACAACAAAACTGCTGTTGAAGGATGGTTGAGCTTCTATTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATGTAAATTCAGAGATATACCCACTGAAAGAAGGCGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCGCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGTGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGATCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGAAGCGACTTTCATTTGATTTGAACTCTAATGATTTGCTACAAAAGCTCTGGGCATTGCATATCGCTATCAAATCTGTGAATGTTTAGTAATATGTATTGTTAGAATCAAATTTCTATCTGTATCTGATTAAAGATTGGGCAATGATCTTATCTTCTGAACTAAATTGATATCTTGCCTATAATGTCATGAATAGTAGCAGAACCTTTCAAGTTCTCGAAAACGGAGTTTGAGCATGGTATGTATAGTTTGTAGTGTTGTGCGGCCGTACCTGAACGGTTTTGGCAAGCTTGATTTCTTTGCAGTTTGGGAGGGTGGAGACTGGCTAATGAAAGATTTAATAAATGACAGATATTTAAGAGGGTATGAGATTTGAACTTCTCACC

Coding sequence (CDS)

ATGGTTGAGCTTCTATTTGACGATATTTTTAAGATTGAGCGATTAAACCCTGATGGTAAAAAGTTTGATAAAGTTACTCGAATAGAGGCAAAGAGCGAGAAGTTTGACATGTTCATGCATCTAGATGTAAATTCAGAGATATACCCACTGAAAGAAGGCGAGAAGTTCTCGATGGCACTAGCTCCAACTCTGAATCTTGATGGAACCCCTGACACTGGATATTTTCTTCAGGGCAATCGGAAGTCGCTTGCAGACAGATATGAATATGTCATGCAAGGGAAGCTGTTCAGAATTTCAGAAAGTTCTGGCCATGGTGGAAAAGCTGAAATAGATGCTTCTTTTGGTGGACTTTTGATGATGCTGAAGGGAGATCCCTCACATTGCAGCAAGTTTGAACTTGACCAGAGGCTATTTCTTCTCATCAGGAAGGTGTGA

Protein sequence

MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKGDPSHCSKFELDQRLFLLIRKV
Homology
BLAST of IVF0002614 vs. ExPASy Swiss-Prot
Match: O81097 (DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702 GN=NRPB8A PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.5e-54
Identity = 96/141 (68.09%), Postives = 121/141 (85.82%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLDVN+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

BLAST of IVF0002614 vs. ExPASy Swiss-Prot
Match: Q9M1A8 (DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=3702 GN=NRPB8B PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 8.5e-54
Identity = 94/141 (66.67%), Postives = 122/141 (86.52%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLDVN+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

BLAST of IVF0002614 vs. ExPASy Swiss-Prot
Match: Q54YW8 (DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium discoideum OX=44689 GN=polr2h PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-31
Identity = 65/143 (45.45%), Postives = 98/143 (68.53%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           M  +LF+D F+++ ++PDGKKFD+V+R    SE ++M + +D+ + IYPL   E+F M L
Sbjct: 1   MTSILFEDTFEVKEIDPDGKKFDRVSRFVCYSENYEMDLQIDIATHIYPLGH-ERFKMVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           A +LNLDG+ D       N+ SLAD+Y+YVM GK+F+  + +    K  + ASFGGLL++
Sbjct: 61  ASSLNLDGSSDNSGSYDTNKPSLADKYDYVMYGKIFKYQKENS-SSKVSVFASFGGLLVL 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRK 144
           L+GDP +    ELD R++LLI+K
Sbjct: 121 LQGDPRYLPGIELDARIYLLIKK 141

BLAST of IVF0002614 vs. ExPASy Swiss-Prot
Match: A8XT81 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis briggsae OX=6238 GN=rpb-8 PE=3 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.5e-29
Identity = 64/145 (44.14%), Postives = 94/145 (64.83%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           M  ++FDD+F ++ ++PDGKKFD+V+R    +E F M + +D+NS+IYPLK+ +KF + L
Sbjct: 1   MAGIIFDDMFSVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKFRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   +S   GG+    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDSGTDGGQLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

BLAST of IVF0002614 vs. ExPASy Swiss-Prot
Match: Q19826 (Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhabditis elegans OX=6239 GN=rpb-8 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 3.2e-29
Identity = 64/145 (44.14%), Postives = 94/145 (64.83%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           M  ++FDD+FK++ ++PDGKKFD+V+R    +E F M + +D+NS+IYPLK+ +K  + L
Sbjct: 1   MAGIIFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRI--SESSGHGGKAEIDASFGGLL 120
           A TL  DG  D G +          +YEYVM GK++R+   ++   GGK    ASFGGLL
Sbjct: 61  ATTLREDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLL 120

Query: 121 MMLKGDPSHCSKFELDQRLFLLIRK 144
           M LKG+  +   FE+D  L+LL++K
Sbjct: 121 MRLKGEAINLHGFEVDMNLYLLMKK 145

BLAST of IVF0002614 vs. ExPASy TrEMBL
Match: A0A1S3BXS2 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN=LOC103494598 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.8e-76
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. ExPASy TrEMBL
Match: A0A0A0KYV5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 6.3e-76
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. ExPASy TrEMBL
Match: A0A6J1EXM3 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3662 GN=LOC111439203 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.9e-74
Identity = 140/144 (97.22%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. ExPASy TrEMBL
Match: A0A6J1CWD5 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015176 PE=3 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.0e-73
Identity = 138/144 (95.83%), Postives = 143/144 (99.31%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGY+LQGNRKSLADRYEY+MQGKLFRISESSGHGG+AEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. ExPASy TrEMBL
Match: A0A6J1I8Y6 (DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=3661 GN=LOC111470753 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 6.5e-73
Identity = 139/144 (96.53%), Postives = 141/144 (97.92%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGH GKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHVGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 145
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. NCBI nr
Match: XP_008454076.1 (PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo])

HSP 1 Score: 291 bits (744), Expect = 3.76e-99
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 144
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. NCBI nr
Match: XP_004152137.1 (DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 hypothetical protein Csa_014470 [Cucumis sativus])

HSP 1 Score: 290 bits (743), Expect = 5.34e-99
Identity = 143/144 (99.31%), Postives = 144/144 (100.00%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIYPLKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYPLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 144
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. NCBI nr
Match: XP_038879439.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_038879448.1 DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida])

HSP 1 Score: 287 bits (734), Expect = 1.26e-97
Identity = 142/144 (98.61%), Postives = 143/144 (99.31%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+NSEIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINSEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 144
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. NCBI nr
Match: XP_022932746.1 (DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_022932747.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_023538873.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo] >XP_023538874.1 DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 284 bits (726), Expect = 2.09e-96
Identity = 140/144 (97.22%), Postives = 142/144 (98.61%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGYFLQGNRKSLADRYEYVM GKLFRISESSGHGGKAEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMHGKLFRISESSGHGGKAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 144
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. NCBI nr
Match: XP_022145813.1 (DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145814.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia] >XP_022145815.1 DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica charantia])

HSP 1 Score: 283 bits (724), Expect = 4.22e-96
Identity = 138/144 (95.83%), Postives = 143/144 (99.31%), Query Frame = 0

Query: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMAL 60
           MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLD+N+EIY LKEGEKFSMAL
Sbjct: 1   MVELLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDINTEIYNLKEGEKFSMAL 60

Query: 61  APTLNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMM 120
           APTLNLDGTPDTGY+LQGNRKSLADRYEY+MQGKLFRISESSGHGG+AEIDASFGGLLMM
Sbjct: 61  APTLNLDGTPDTGYYLQGNRKSLADRYEYIMQGKLFRISESSGHGGRAEIDASFGGLLMM 120

Query: 121 LKGDPSHCSKFELDQRLFLLIRKV 144
           LKGDPSHCSKFELDQRLFLLIRKV
Sbjct: 121 LKGDPSHCSKFELDQRLFLLIRKV 144

BLAST of IVF0002614 vs. TAIR 10
Match: AT1G54250.1 (RNA polymerase Rpb8 )

HSP 1 Score: 211.5 bits (537), Expect = 4.6e-55
Identity = 96/141 (68.09%), Postives = 121/141 (85.82%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPT 63
           +LF+DIF +++L+PDGKKFDKVTR++A S   +MFMHLDVN+E+YPL  G+KF++ALAPT
Sbjct: 6   ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+LKG
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

BLAST of IVF0002614 vs. TAIR 10
Match: AT3G59600.1 (RNA polymerase Rpb8 )

HSP 1 Score: 211.1 bits (536), Expect = 6.0e-55
Identity = 94/141 (66.67%), Postives = 122/141 (86.52%), Query Frame = 0

Query: 4   LLFDDIFKIERLNPDGKKFDKVTRIEAKSEKFDMFMHLDVNSEIYPLKEGEKFSMALAPT 63
           ++F+DIF +++L+PDGKKFDKVTR+EA+S   +MFMHLDVN+E+YPL  G+KF++A+APT
Sbjct: 6   IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65

Query: 64  LNLDGTPDTGYFLQGNRKSLADRYEYVMQGKLFRISESSGHGGKAEIDASFGGLLMMLKG 123
           LNLDGTPDTGYF  G +K+LAD+YEY+M GKL++ISE  G   KAE+  SFGGLLM+L+G
Sbjct: 66  LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125

Query: 124 DPSHCSKFELDQRLFLLIRKV 145
           DP+H S FELDQRLFLL+RK+
Sbjct: 126 DPAHISHFELDQRLFLLMRKL 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O810976.5e-5468.09DNA-directed RNA polymerases II and V subunit 8A OS=Arabidopsis thaliana OX=3702... [more]
Q9M1A88.5e-5466.67DNA-directed RNA polymerases II, IV and V subunit 8B OS=Arabidopsis thaliana OX=... [more]
Q54YW81.2e-3145.45DNA-directed RNA polymerases I, II, and III subunit rpabc3 OS=Dictyostelium disc... [more]
A8XT812.5e-2944.14Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
Q198263.2e-2944.14Probable DNA-directed RNA polymerases I, II, and III subunit RPABC3 OS=Caenorhab... [more]
Match NameE-valueIdentityDescription
A0A1S3BXS24.8e-76100.00DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucumis melo OX=3656 GN... [more]
A0A0A0KYV56.3e-7699.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G011050 PE=3 SV=1[more]
A0A6J1EXM35.9e-7497.22DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita moschata OX=3... [more]
A0A6J1CWD51.0e-7395.83DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 OS=Momordica ch... [more]
A0A6J1I8Y66.5e-7396.53DNA-directed RNA polymerases II and V subunit 8A-like OS=Cucurbita maxima OX=366... [more]
Match NameE-valueIdentityDescription
XP_008454076.13.76e-99100.00PREDICTED: DNA-directed RNA polymerases II and V subunit 8A-like [Cucumis melo][more]
XP_004152137.15.34e-9999.31DNA-directed RNA polymerases II and V subunit 8A [Cucumis sativus] >KGN53006.1 h... [more]
XP_038879439.11.26e-9798.61DNA-directed RNA polymerases II and V subunit 8A-like [Benincasa hispida] >XP_03... [more]
XP_022932746.12.09e-9697.22DNA-directed RNA polymerases II and V subunit 8A-like [Cucurbita moschata] >XP_0... [more]
XP_022145813.14.22e-9695.83DNA-directed RNA polymerases II and V subunit 8A-like isoform X2 [Momordica char... [more]
Match NameE-valueIdentityDescription
AT1G54250.14.6e-5568.09RNA polymerase Rpb8 [more]
AT3G59600.16.0e-5566.67RNA polymerase Rpb8 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005570RNA polymerase, Rpb8SMARTSM00658rpol8neucoord: 2..143
e-value: 7.3E-68
score: 241.4
IPR005570RNA polymerase, Rpb8PIRSFPIRSF000779RPB8coord: 1..144
e-value: 8.0E-50
score: 166.6
IPR005570RNA polymerase, Rpb8PFAMPF03870RNA_pol_Rpb8coord: 7..143
e-value: 1.8E-49
score: 167.5
IPR005570RNA polymerase, Rpb8PANTHERPTHR10917DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3coord: 1..144
NoneNo IPR availableGENE3D2.40.50.140coord: 6..144
e-value: 3.8E-56
score: 191.1
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 4..143

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0002614.2IVF0002614.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity