IVF0002490 (gene) Melon (IVF77) v1

Overview
NameIVF0002490
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionshort-chain dehydrogenase TIC 32, chloroplastic-like
Locationchr09: 23161417 .. 23165110 (-)
RNA-Seq ExpressionIVF0002490
SyntenyIVF0002490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCCCCTCCAAAAAATCCTTAAACGAAGCAATTTGCACTTGGCGAGTGGCCGTGACGATTGATCGGATAGAAAGAGAGGAATGTGGTTTGTTAACAGGAAGGGGCCATCTGGGTTCTCAGCTTCTTCCACGGCTGAAGAAGTTACACAAGGAATCAATGGAAATGGCCTCACAGCCATTGTTACAGGTCACTTTCCACCTTACTTCATTTCTCTATTGTTGAATTGATTAAACAAATCGCCCTATTTCTTATGAATTTTGTTTCTGTTACTTATCAGAGGTTAAGGGGTTACTGGATTTCATTCCTTTTAAATGGGAGAACCTGTATTTGCACGTATCATTGATTGAAAATATGATATGCCCAAATGGCCAAAATGTTTCACAGCTATGTATTATACAATGTTTTGCTTTGTTGATTGTCCAAATTATATATTCACTGATCAAAAAGGTAAATTAATGTGTAGACAATGAGCATAATCCTGAGGGTTTTGGTTGCCTTTGGTGTTTGATGAAAATTAAAGTTTCAGTTTTTGTGGAGCTTGGATTTTCTCATTATAAGGTCTTCTGCATTAACACAGGTGCTTCAAGTGGCATTGGCAGTGAAACTGCTCGTGTTCTTGCACTGCGCGGAGTACATGTAATAATGGCGGTTAGAAATGTAGCAACTGGTAGGGAGGTTCAAAAAGCCATTGTCAAGGAAAACCCAACTGCCAAAGTTGACACCATGGAGTTGGACCTGAGCTCAATGGCATCTGTTCGGAATTTTACATCAAATTTCAAGTCCTCAGGTCTTCCACTGAATATTCTTGTGTAAGATGGCGAACTGCATGAATTTCTCTGCTACTTTTGCGATCTGAATATATGGATCTTCTTATTTCTTCATCTAGCCAATGATCTTCATGTATAAGCTGTCCTTGCCTAAGTGCTTTTATATGTGCACGTATATGCTATTAGCATAAAGAGGAGAAAGCTGAACATTTTCGCGATTCGTAGTTATCCTTTTTTTTTGTTCGTTCAGTAATAATGCAGGAGTCATGGCTTCACCCTTCTTGCTCTCGAAGGACAAGCTAGAATTGCAGTTTGCAACAAACCATGTGGGTAAGTACATCATCAATAGAGGACTGATTGCTGCTCTCTTTTGTAGTGGATGGTAGGAAATTGATGGTCTAGTCATTCTTGTTCTATGGTGCCCTAATTCGTGATAGTTCTTTTTGCAGGACATTTTCTTTTGACAAATCTATTGTTGGAGACATTGAAAAAGACAGCAAAAGATAGTGAAAGAGAAGGAAGGATTGTCAATGTCTCGTCACGACGGCACCAATTTTCGTATCGTGAAGGCATCCGATTTGACAAAATCAACGATCAATCAGGGTCCTCTTTCTATCACCCTTTGCTATAGTTCTTTTTCTCCCAGCATAAGCTAAATTCACGGTTCCTTCAGAACATATTTGTTCTGCTTAGAGGTTTGTGAAGAGTCTGAGGGATGTCCACAACATTAAATATGAGGATTATTTCCTTGTTATTTCTTGGCTTATAACTTCAAATTGAGGAAATTTAGAATCAAATTCCTGCATAATGTCGTTGCTATGCTTTGGTTGTGGCGAATACTGTATCAAATTTGAGCCATATAATCTCTACTGATTCATTCTATACAGCTATAATGGTCTATCTGCGTATGGTCAATCAAAGCTTGCTAATATTTTGCATGCTTCCGAACTTGCAAGACAGCTGAAGGTTCGTGACATGTTTGTTAAATTTTCTCTTTTTATAAATTTAGGCAATCAAAGAATGTAACGGATTGTATTATTATCATTTAGAGAATACATAGTTTCCTTTCGGCTGACAAAGAACGTTTTAAAACATTTGGAAGTTTGGGTTGAGCAGTAATTTAACATAATCTTAAATTAGGATGTCTTAGGTTCAAGTTCTCGTTGCACATTGTTGCCTTCAAATGATTATCGATAATCCACTTGTTGGGTCTCTCTTGTTTCAACTCTACAAACAAGGGAAGTGTTAAGCACTCTTAAGGGTTTATATATCGAGTAGTTTAACACTTTTCAGTTTTCATTGAAACCAGGCTTTGGTGTTGTTTCTACCATTTTTTGCCTTTTCTATCCTTTTAGGGCAAACAGACACTATTACCAGGCAATCGTTTGCTTTTTTAAACAGGAAGAAGGGGTGAAGATCACTGCAAATTCACTACATCCTGGAGCCATTCCTACTAACCTATTTCGTTATCATAGCCTCATCAACGGTACTAAATCTTTTATTGTACTATTTTTGGTATCGTCATCTAATCATTTTCATAAAGCTCTGAATTTCAACTTGCAATACTGTGCTCCTTGACACTAATAACATGTTTTTCACATCTCCAAGGTCTTTTTACCTGTTTCTCAATATGCTTTGTTGCAGTATTACCTTTTTTTTTTTTAATTTATTGTTTCAGTGTTGTCTTACTGCTACATCACACGTCTTTATAAAAATTATTTTCTCTGTCTTTGTCCCGAATTTTGTGCAGGTTTTCTTGGCGTTCTGGGTAAACATGTCATGAAGAATGTACAACAGGTAATGGTCTATTTCAGAAAAAGCTATATGCACAAATTTGTAGGCCTTTCACTTCTGCATGTTTAATAAATCAACTTGATTACAAAATTTAGGCTTCTGTTAAGGGTTTTGCTACTTTTGACCGATAGATTTTCGGGTTTCCAGAACCCTAACCTATTTTAGTAAAATCCTACGCAGAGATCTGAATCTCCAAATTGATAAAAAAAAAAAAAAATAGCACATGCTAATTACTATTGAACTATGCTATATTGGTGATTTTAATCCTATTATGTTCTATAACTTTTTTGTAATTATTTGGTCTTAATTTCTAAACCTTAAAGGCTGTGTTAATAGTTGCTAAATTTTGTATAGAAAATATTTCAGTAAAACCATTTGGTTGTGTTTTGGGACTCATTGTCCTGCTTTTTTAACAAATCTCAAAATTAGTCTTAAATTACTTTTATATAATTTGGTAAAATAATAATAAAAATTCTTATTCAAGAAAGAAATTAATAAATAAATAAACAAAAAAAGATGTTTGTGTTAAATTTTTGTTAAATAGTTTATTAAAAATTAAAAATAGTTTTTCTAATGAAAAATTCATGAACAAGAATGGAAGTTGAATCATTTTTATAAGTGAGCCCGGTTTCTATCAAATTCATTCATTTAACAAATTTGATAGCCTCCCTAGTTGCTCCTGCCTTTGGTATGGGAACAATTATGTTTCCCATTTGAACTTTTCAATTAGACGATCCTAGTCGTGAACATTCTATCCTTTAAAATTAGAAAGTCACCTTTAAAATTAGAAAGTCAAAAACACGATGGATTTATATGAACAGTGAAGAAATAGTTGTGATTATTGTTCCGTTTTCAGGGAGCAGCGACGACATGCTATGTTGCATTGCATCCACAAGTCAATGGGATCAGTGCCCAGTATTTTGCTGACAGTAACATTGCCAAAGCAAGTTCACAAGCCAATGATAGTGAATTGGCAAAGAGACTGTGGGATTTCACCCAGAAGTTGGTATCTAGTGCTTTACAAATTCCTTAAAAGCTTCTATAATGCACTCCTCATTCATCTGTGAATTTGAATAAATTTGTAAATCAAACATCTCCAACTGTTGAGATTTTCAGTTTACATCCTGTGTTTA

mRNA sequence

GTCCCCTCCAAAAAATCCTTAAACGAAGCAATTTGCACTTGGCGAGTGGCCGTGACGATTGATCGGATAGAAAGAGAGGAATGTGGTTTGTTAACAGGAAGGGGCCATCTGGGTTCTCAGCTTCTTCCACGGCTGAAGAAGTTACACAAGGAATCAATGGAAATGGCCTCACAGCCATTGTTACAGGTGCTTCAAGTGGCATTGGCAGTGAAACTGCTCGTGTTCTTGCACTGCGCGGAGTACATGTAATAATGGCGGTTAGAAATGTAGCAACTGGTAGGGAGGTTCAAAAAGCCATTGTCAAGGAAAACCCAACTGCCAAAGTTGACACCATGGAGTTGGACCTGAGCTCAATGGCATCTGTTCGGAATTTTACATCAAATTTCAAGTCCTCAGGTCTTCCACTGAATATTCTTGTTAATAATGCAGGAGTCATGGCTTCACCCTTCTTGCTCTCGAAGGACAAGCTAGAATTGCAGTTTGCAACAAACCATGTGGGACATTTTCTTTTGACAAATCTATTGTTGGAGACATTGAAAAAGACAGCAAAAGATAGTGAAAGAGAAGGAAGGATTGTCAATGTCTCGTCACGACGGCACCAATTTTCGTATCGTGAAGGCATCCGATTTGACAAAATCAACGATCAATCAGGCTATAATGGTCTATCTGCGTATGGTCAATCAAAGCTTGCTAATATTTTGCATGCTTCCGAACTTGCAAGACAGCTGAAGGAAGAAGGGGTGAAGATCACTGCAAATTCACTACATCCTGGAGCCATTCCTACTAACCTATTTCGTTATCATAGCCTCATCAACGGTTTTCTTGGCGTTCTGGGTAAACATGTCATGAAGAATGTACAACAGGGAGCAGCGACGACATGCTATGTTGCATTGCATCCACAAGTCAATGGGATCAGTGCCCAGTATTTTGCTGACAGTAACATTGCCAAAGCAAGTTCACAAGCCAATGATAGTGAATTGGCAAAGAGACTGTGGGATTTCACCCAGAAGTTGGTATCTAGTGCTTTACAAATTCCTTAAAAGCTTCTATAATGCACTCCTCATTCATCTGTGAATTTGAATAAATTTGTAAATCAAACATCTCCAACTGTTGAGATTTTCAGTTTACATCCTGTGTTTA

Coding sequence (CDS)

ATGTGGTTTGTTAACAGGAAGGGGCCATCTGGGTTCTCAGCTTCTTCCACGGCTGAAGAAGTTACACAAGGAATCAATGGAAATGGCCTCACAGCCATTGTTACAGGTGCTTCAAGTGGCATTGGCAGTGAAACTGCTCGTGTTCTTGCACTGCGCGGAGTACATGTAATAATGGCGGTTAGAAATGTAGCAACTGGTAGGGAGGTTCAAAAAGCCATTGTCAAGGAAAACCCAACTGCCAAAGTTGACACCATGGAGTTGGACCTGAGCTCAATGGCATCTGTTCGGAATTTTACATCAAATTTCAAGTCCTCAGGTCTTCCACTGAATATTCTTGTTAATAATGCAGGAGTCATGGCTTCACCCTTCTTGCTCTCGAAGGACAAGCTAGAATTGCAGTTTGCAACAAACCATGTGGGACATTTTCTTTTGACAAATCTATTGTTGGAGACATTGAAAAAGACAGCAAAAGATAGTGAAAGAGAAGGAAGGATTGTCAATGTCTCGTCACGACGGCACCAATTTTCGTATCGTGAAGGCATCCGATTTGACAAAATCAACGATCAATCAGGCTATAATGGTCTATCTGCGTATGGTCAATCAAAGCTTGCTAATATTTTGCATGCTTCCGAACTTGCAAGACAGCTGAAGGAAGAAGGGGTGAAGATCACTGCAAATTCACTACATCCTGGAGCCATTCCTACTAACCTATTTCGTTATCATAGCCTCATCAACGGTTTTCTTGGCGTTCTGGGTAAACATGTCATGAAGAATGTACAACAGGGAGCAGCGACGACATGCTATGTTGCATTGCATCCACAAGTCAATGGGATCAGTGCCCAGTATTTTGCTGACAGTAACATTGCCAAAGCAAGTTCACAAGCCAATGATAGTGAATTGGCAAAGAGACTGTGGGATTTCACCCAGAAGTTGGTATCTAGTGCTTTACAAATTCCTTAA

Protein sequence

MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAVRNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMASPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREGIRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRYHSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSELAKRLWDFTQKLVSSALQIP
Homology
BLAST of IVF0002490 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-118
Identity = 212/313 (67.73%), Postives = 259/313 (82.75%), Query Frame = 0

Query: 1   MW-FVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMA 60
           MW F ++KG SGFS SSTAE+VT GI+  GLTAIVTGASSGIG+ET RVLALRG HVIM 
Sbjct: 1   MWPFSSKKGVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMG 60

Query: 61  VRNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVM 120
           VRN+   ++V+  I+K+ P+AKVD +ELDLSS+ SV+ F S F SSG PLNIL+NNAG+M
Sbjct: 61  VRNMVAAKDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIM 120

Query: 121 ASPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYRE 180
           A PF LSKD +ELQFATNH+GHFLLTNLLL+T+KKT ++S++EGRIVNV+S  H+F+Y E
Sbjct: 121 ACPFKLSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPE 180

Query: 181 GIRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFR 240
           GIRFDKINDQS YN   AYGQSKLAN+LHA++L + LKE+GV ITANSLHPG I TNLFR
Sbjct: 181 GIRFDKINDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR 240

Query: 241 YHSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSE 300
           ++S +NG + V+GK V+KNVQQGAATTCYVALHPQV G+S +YF+DSN+ K +    D +
Sbjct: 241 HNSAVNGLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVD 300

Query: 301 LAKRLWDFTQKLV 313
           LAK+LWDF+  LV
Sbjct: 301 LAKKLWDFSINLV 313

BLAST of IVF0002490 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 7.7e-116
Identity = 210/315 (66.67%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF   KG SGFS+ STAEEVT G++G GLTAIVTGASSGIG ETARVL+LRGVHV+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RN  +G +V++ IVK+ P AK+D MELDLSSM SVR F S +KS+GLPLN+L+NNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
            PF+LSKD +ELQFATNH+GHFLLT LLL+T+K T+++S+REGRIVN+SS  H+FSY EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           +RFDKIND+S Y+ + AYGQSKL N+LHA+EL +QLKE+GV ITANSLHPGAI TNL RY
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 -HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSE 300
            +  +   +G + K+++K+V QGAATTCYVAL+PQV G+S +YF DSNIAK      D+E
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKRLWDFTQKLVSS 315
           LAK++WDF+ KL  S
Sbjct: 301 LAKKVWDFSTKLTDS 315

BLAST of IVF0002490 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.6e-89
Identity = 168/309 (54.37%), Postives = 224/309 (72.49%), Query Frame = 0

Query: 6   RKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAVRNVAT 65
           + G SG+ ++STAE+VT  I+   LTAI+TG +SGIG E ARVL +RG HVI+A RN   
Sbjct: 10  KAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAARNTKA 69

Query: 66  GREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMASPFLL 125
             + ++ I++  P A++D ++LDLSS+ SVR+F   F +  +PLNIL+NNAGVM  PF L
Sbjct: 70  ANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQL 129

Query: 126 SKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREGIRFDK 185
           S+D +E QFATNH+GHFLLTNLLL+ +K +A++S  EGRIVN+SS  H ++Y EGI FD 
Sbjct: 130 SEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDY 189

Query: 186 INDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRYHSLIN 245
           IND   Y+   AYGQSKLAN+LH++ L+R+L+EEGV IT NS+HPG I TNLFR+  L  
Sbjct: 190 INDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSGLGM 249

Query: 246 GFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSELAKRLW 305
             L  +   + KN+ QGAATTCYVALHP + G++ +YF D N+   S+ A D+ LA +LW
Sbjct: 250 AVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTLADKLW 309

Query: 306 DFTQKLVSS 315
           DF+ KLV S
Sbjct: 310 DFSIKLVDS 318

BLAST of IVF0002490 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 327.8 bits (839), Expect = 1.4e-88
Identity = 168/309 (54.37%), Postives = 224/309 (72.49%), Query Frame = 0

Query: 6   RKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAVRNVAT 65
           + G SG+ ++STAE+VT  I+   LTAI+TG +SGIG E ARVL +RG HVI+A RN   
Sbjct: 10  KAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASRNTKA 69

Query: 66  GREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMASPFLL 125
             + ++ I++  P A++D ++LDLSS+ SVR+F   F +  +PLNIL+NNAGVM  PF L
Sbjct: 70  ANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFCPFQL 129

Query: 126 SKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREGIRFDK 185
           S+D +E QFATNH+GHFLLTNLLL+ +K +A++S  EGRIVN+SS  H ++Y EGI FD 
Sbjct: 130 SEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGIMFDY 189

Query: 186 INDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRYHSLIN 245
           IND   Y+   AYGQSKLAN+LH++ L+R+L+EEGV IT NS+HPG I TNLFR+  L  
Sbjct: 190 INDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFRHSGLGM 249

Query: 246 GFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSELAKRLW 305
             L  +   + KN+ QGAATTCYVALHP +  ++ +YFAD N+   S+ A D+ LA +LW
Sbjct: 250 AVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTLADKLW 309

Query: 306 DFTQKLVSS 315
           DF+ KLV S
Sbjct: 310 DFSIKLVES 318

BLAST of IVF0002490 vs. ExPASy Swiss-Prot
Match: Q91WL8 (WW domain-containing oxidoreductase OS=Mus musculus OX=10090 GN=Wwox PE=1 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.6e-49
Identity = 123/321 (38.32%), Postives = 180/321 (56.07%), Query Frame = 0

Query: 12  FSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAVRNVATGREVQK 71
           +  S+TA E+ QG +  G   +VTGA+SGIG ETA+  AL G HVI+A RN++   E   
Sbjct: 107 YDGSTTAMEILQGRDFTGKVVLVTGANSGIGFETAKSFALHGAHVILACRNLSRASEAVS 166

Query: 72  AIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMASPFLLSKDKLE 131
            I++E   AKV+ M LDL+ + SV++F   FK+  + L++LV NAG  A P+ L+KD LE
Sbjct: 167 RILEEWHKAKVEAMTLDLAVLRSVQHFAEAFKAKNVSLHVLVCNAGTFALPWGLTKDGLE 226

Query: 132 LQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREGIRFDKINDQSG 191
             F  NH+GHF L  LL + L +++       R++ VSS  H        RF  IND SG
Sbjct: 227 TTFQVNHLGHFYLVQLLQDVLCRSS-----PARVIVVSSESH--------RFTDINDSSG 286

Query: 192 -------------YNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLF 251
                        Y  + AY +SKL NIL ++EL R+L   GV  T+N++HPG +  +  
Sbjct: 287 KLDLSRLSPPRSDYWAMLAYNRSKLCNILFSNELHRRLSPRGV--TSNAVHPGNMMYSAI 346

Query: 252 RYHSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDS 311
             +S +   L  L +   K++QQGAATT Y A+ P++ G+   YF +      S +A   
Sbjct: 347 HRNSWVYKLLFTLARPFTKSMQQGAATTVYCAVAPELEGLGGMYFNNCCRCLPSEEAQSE 406

Query: 312 ELAKRLWDFTQKLVSSALQIP 320
           E A+ LW+ +++L+   L  P
Sbjct: 407 ETARALWELSERLIQDRLGSP 412

BLAST of IVF0002490 vs. ExPASy TrEMBL
Match: A0A5A7TIJ9 (Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001080 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.0e-171
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV
Sbjct: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL
Sbjct: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 320
           AKRLWDFTQKLVSSALQIP
Sbjct: 301 AKRLWDFTQKLVSSALQIP 319

BLAST of IVF0002490 vs. ExPASy TrEMBL
Match: A0A1S3AUT5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103482904 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 4.3e-170
Identity = 315/319 (98.75%), Postives = 318/319 (99.69%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIG+ETARVLALRGVHVIMAV
Sbjct: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGTETARVLALRGVHVIMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQK IVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQKVIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           HSLI+GFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL
Sbjct: 241 HSLIDGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 320
           AKRLWDFTQKLVSSALQIP
Sbjct: 301 AKRLWDFTQKLVSSALQIP 319

BLAST of IVF0002490 vs. ExPASy TrEMBL
Match: A0A0A0KNI0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141110 PE=3 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 9.4e-165
Identity = 303/319 (94.98%), Postives = 313/319 (98.12%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFV+RKGPSGFSASSTAEEVTQGING GLTAIVTGASSGIG+ETARVLALRGVHV+MAV
Sbjct: 1   MWFVSRKGPSGFSASSTAEEVTQGINGTGLTAIVTGASSGIGTETARVLALRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQ+AI+KENPTAKVDTMELDLSSMASVRNF SNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQEAIIKENPTAKVDTMELDLSSMASVRNFASNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTAK+SEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTAKNSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H+LINGFLGVLGKHVMKNVQQGAATTCYVALH Q+NGIS QYFADSNIAKA+SQANDSEL
Sbjct: 241 HNLINGFLGVLGKHVMKNVQQGAATTCYVALHQQINGISGQYFADSNIAKANSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 320
           AKRLWDFTQKLVSS LQ P
Sbjct: 301 AKRLWDFTQKLVSSGLQTP 319

BLAST of IVF0002490 vs. ExPASy TrEMBL
Match: A0A6J1E3K6 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430279 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 1.8e-155
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF++RKGPSGFSASSTAEEVT+GI+G GL AIVTGASSGIGSETARVLALRGV V+MAV
Sbjct: 1   MWFLSRKGPSGFSASSTAEEVTEGIDGTGLIAIVTGASSGIGSETARVLALRGVRVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGR+VQ+AIVKENPTAKVDTMELDLSSMASVRNF SNF SSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGRKVQEAIVKENPTAKVDTMELDLSSMASVRNFASNFNSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTA +S++EGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTATESKKEGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKIND SGYNGLSAYGQSKLANILHASELARQLKEEGV ITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDPSGYNGLSAYGQSKLANILHASELARQLKEEGVNITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H LINGFLG++GKHVMKNVQQGAATTCYVALHPQ+ GIS QYFAD N+AKASSQANDSEL
Sbjct: 241 HGLINGFLGLVGKHVMKNVQQGAATTCYVALHPQMKGISGQYFADGNVAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSA 316
           AK+LWDFTQKL+S+A
Sbjct: 301 AKKLWDFTQKLISTA 315

BLAST of IVF0002490 vs. ExPASy TrEMBL
Match: A0A6J1I6C2 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111471521 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.4e-154
Identity = 282/313 (90.10%), Postives = 300/313 (95.85%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF++RKGPSGFSASSTAEEVT+GI+G GLTAIVTGASSGIGSETARVLALRGV V+MAV
Sbjct: 1   MWFLSRKGPSGFSASSTAEEVTEGIDGTGLTAIVTGASSGIGSETARVLALRGVRVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGR+VQ+AIVKENPTAKVDTMELDLSSMASVR F SNF SSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGRKVQEAIVKENPTAKVDTMELDLSSMASVRKFASNFNSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTA +S++EGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTATESKKEGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKIND SGYNGLSAYGQSKLANILHASELARQLKEEGV ITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDPSGYNGLSAYGQSKLANILHASELARQLKEEGVNITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H LINGFLG++GKHVMKNVQQGA TTCYVALHPQ+ GIS QYFAD N+AKASSQANDSEL
Sbjct: 241 HGLINGFLGLVGKHVMKNVQQGAVTTCYVALHPQMKGISGQYFADGNVAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVS 314
           A++LWDFTQKL+S
Sbjct: 301 ARKLWDFTQKLIS 313

BLAST of IVF0002490 vs. NCBI nr
Match: KAA0042608.1 (short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa] >TYK06010.1 short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa])

HSP 1 Score: 613 bits (1582), Expect = 4.38e-221
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV
Sbjct: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL
Sbjct: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 319
           AKRLWDFTQKLVSSALQIP
Sbjct: 301 AKRLWDFTQKLVSSALQIP 319

BLAST of IVF0002490 vs. NCBI nr
Match: XP_008437509.1 (PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo])

HSP 1 Score: 608 bits (1568), Expect = 5.96e-219
Identity = 315/319 (98.75%), Postives = 318/319 (99.69%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIG+ETARVLALRGVHVIMAV
Sbjct: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGTETARVLALRGVHVIMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQK IVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQKVIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           HSLI+GFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL
Sbjct: 241 HSLIDGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 319
           AKRLWDFTQKLVSSALQIP
Sbjct: 301 AKRLWDFTQKLVSSALQIP 319

BLAST of IVF0002490 vs. NCBI nr
Match: XP_004145893.1 (short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Cucumis sativus] >KGN49937.1 hypothetical protein Csa_000242 [Cucumis sativus])

HSP 1 Score: 590 bits (1522), Expect = 6.11e-212
Identity = 303/319 (94.98%), Postives = 313/319 (98.12%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWFV+RKGPSGFSASSTAEEVTQGING GLTAIVTGASSGIG+ETARVLALRGVHV+MAV
Sbjct: 1   MWFVSRKGPSGFSASSTAEEVTQGINGTGLTAIVTGASSGIGTETARVLALRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGREVQ+AI+KENPTAKVDTMELDLSSMASVRNF SNFKSSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGREVQEAIIKENPTAKVDTMELDLSSMASVRNFASNFKSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTAK+SEREGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTAKNSEREGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H+LINGFLGVLGKHVMKNVQQGAATTCYVALH Q+NGIS QYFADSNIAKA+SQANDSEL
Sbjct: 241 HNLINGFLGVLGKHVMKNVQQGAATTCYVALHQQINGISGQYFADSNIAKANSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSALQIP 319
           AKRLWDFTQKLVSS LQ P
Sbjct: 301 AKRLWDFTQKLVSSGLQTP 319

BLAST of IVF0002490 vs. NCBI nr
Match: XP_023549790.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 562 bits (1448), Expect = 1.11e-200
Identity = 286/315 (90.79%), Postives = 304/315 (96.51%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF++RKGPSGFSASSTAEEVT+GI+G GLTAIVTGASSGIGSETARVLALRGV V+MAV
Sbjct: 1   MWFLSRKGPSGFSASSTAEEVTEGIDGTGLTAIVTGASSGIGSETARVLALRGVRVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGR+VQ+AIVKENPTAKVDTMELDLSSMASVRNF SNF SSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGRKVQEAIVKENPTAKVDTMELDLSSMASVRNFASNFNSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTA +S++EGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTATESKKEGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKIND SGYNGLSAYGQSKLANILHASELARQLKEEGV ITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDPSGYNGLSAYGQSKLANILHASELARQLKEEGVNITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H LINGFLG++GKHVMKNVQQGAATTCYVALHPQ+ GIS QYFAD N+AKASSQANDSEL
Sbjct: 241 HGLINGFLGLVGKHVMKNVQQGAATTCYVALHPQMKGISGQYFADGNVAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSA 315
           AK+LWDFTQKL+S+A
Sbjct: 301 AKKLWDFTQKLISTA 315

BLAST of IVF0002490 vs. NCBI nr
Match: XP_022922236.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 560 bits (1442), Expect = 9.13e-200
Identity = 285/315 (90.48%), Postives = 303/315 (96.19%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF++RKGPSGFSASSTAEEVT+GI+G GL AIVTGASSGIGSETARVLALRGV V+MAV
Sbjct: 1   MWFLSRKGPSGFSASSTAEEVTEGIDGTGLIAIVTGASSGIGSETARVLALRGVRVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RNVATGR+VQ+AIVKENPTAKVDTMELDLSSMASVRNF SNF SSGLPLNILVNNAGVMA
Sbjct: 61  RNVATGRKVQEAIVKENPTAKVDTMELDLSSMASVRNFASNFNSSGLPLNILVNNAGVMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
           SPFLLSKDK+ELQFATNHVGHFLLTNLLLETLKKTA +S++EGRIVNVSSRRHQFSYREG
Sbjct: 121 SPFLLSKDKIELQFATNHVGHFLLTNLLLETLKKTATESKKEGRIVNVSSRRHQFSYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           IRFDKIND SGYNGLSAYGQSKLANILHASELARQLKEEGV ITANSLHPGAIPTNLFRY
Sbjct: 181 IRFDKINDPSGYNGLSAYGQSKLANILHASELARQLKEEGVNITANSLHPGAIPTNLFRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           H LINGFLG++GKHVMKNVQQGAATTCYVALHPQ+ GIS QYFAD N+AKASSQANDSEL
Sbjct: 241 HGLINGFLGLVGKHVMKNVQQGAATTCYVALHPQMKGISGQYFADGNVAKASSQANDSEL 300

Query: 301 AKRLWDFTQKLVSSA 315
           AK+LWDFTQKL+S+A
Sbjct: 301 AKKLWDFTQKLISTA 315

BLAST of IVF0002490 vs. TAIR 10
Match: AT4G23420.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.4e-119
Identity = 213/315 (67.62%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF   KG SGFS+ STAEEVT G++G GLTAIVTGASSGIG ETARVLALRGVHV+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RN   G +V++ IVK+ P AKVD MEL+LSSM SVR F S +KS+GLPLN+L+NNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
            PF+LSKD +ELQFATNH+GHFLLT LLL+T+K T+++S+REGRIVNVSS  H++SY EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           +RFDKIND+S Y+ + AYGQSKL N+LHA+ELA+QLKE+GV ITANSLHPGAI TNL+ Y
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 -HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSE 300
            +S + G +G + K+++K+V QGAATTCYVAL+PQV G++ +YF+DSNIAK      D+E
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKRLWDFTQKLVSS 315
           LAK+LWDF+ KL  S
Sbjct: 301 LAKKLWDFSTKLTDS 315

BLAST of IVF0002490 vs. TAIR 10
Match: AT4G23420.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.4e-119
Identity = 213/315 (67.62%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF   KG SGFS+ STAEEVT G++G GLTAIVTGASSGIG ETARVLALRGVHV+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RN   G +V++ IVK+ P AKVD MEL+LSSM SVR F S +KS+GLPLN+L+NNAG+MA
Sbjct: 61  RNTGAGAKVKEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
            PF+LSKD +ELQFATNH+GHFLLT LLL+T+K T+++S+REGRIVNVSS  H++SY EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           +RFDKIND+S Y+ + AYGQSKL N+LHA+ELA+QLKE+GV ITANSLHPGAI TNL+ Y
Sbjct: 181 VRFDKINDESSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGY 240

Query: 241 -HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSE 300
            +S + G +G + K+++K+V QGAATTCYVAL+PQV G++ +YF+DSNIAK      D+E
Sbjct: 241 FNSYLAGAVGAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTE 300

Query: 301 LAKRLWDFTQKLVSS 315
           LAK+LWDF+ KL  S
Sbjct: 301 LAKKLWDFSTKLTDS 315

BLAST of IVF0002490 vs. TAIR 10
Match: AT4G23430.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 418.3 bits (1074), Expect = 5.5e-117
Identity = 210/315 (66.67%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MWF   KG SGFS+ STAEEVT G++G GLTAIVTGASSGIG ETARVL+LRGVHV+MAV
Sbjct: 1   MWFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RN  +G +V++ IVK+ P AK+D MELDLSSM SVR F S +KS+GLPLN+L+NNAG+MA
Sbjct: 61  RNTDSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
            PF+LSKD +ELQFATNH+GHFLLT LLL+T+K T+++S+REGRIVN+SS  H+FSY EG
Sbjct: 121 CPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           +RFDKIND+S Y+ + AYGQSKL N+LHA+EL +QLKE+GV ITANSLHPGAI TNL RY
Sbjct: 181 VRFDKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRY 240

Query: 241 -HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSE 300
            +  +   +G + K+++K+V QGAATTCYVAL+PQV G+S +YF DSNIAK      D+E
Sbjct: 241 FNPYLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTE 300

Query: 301 LAKRLWDFTQKLVSS 315
           LAK++WDF+ KL  S
Sbjct: 301 LAKKVWDFSTKLTDS 315

BLAST of IVF0002490 vs. TAIR 10
Match: AT4G11410.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 1.6e-116
Identity = 210/311 (67.52%), Postives = 252/311 (81.03%), Query Frame = 0

Query: 1   MWFVNRKGPSGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAV 60
           MW    KG SGFSA STAEEVT GI+G GLTAIVTGASSGIG ET RVLALRGVHV+MAV
Sbjct: 1   MWPFWWKGASGFSARSTAEEVTHGIDGTGLTAIVTGASSGIGEETTRVLALRGVHVVMAV 60

Query: 61  RNVATGREVQKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMA 120
           RN  +G +V+  I+KE P AK+D M+LDLSSMASVR+F S ++S  LPLN+L+NNAG+MA
Sbjct: 61  RNTDSGNQVRDKILKEIPQAKIDVMKLDLSSMASVRSFASEYQSLDLPLNLLINNAGIMA 120

Query: 121 SPFLLSKDKLELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREG 180
            PFLLS D +ELQFATNH+GHFLLTNLLLE +KKTA +S REGRIV VSS  H+F+YREG
Sbjct: 121 CPFLLSSDNIELQFATNHLGHFLLTNLLLERMKKTASESNREGRIVIVSSEGHRFAYREG 180

Query: 181 IRFDKINDQSGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY 240
           ++FDKIND++ YN L AYGQSKL NILHA+ELAR  KE+GV ITANSLHPG+I TNL RY
Sbjct: 181 VQFDKINDEARYNTLQAYGQSKLGNILHATELARLFKEQGVNITANSLHPGSIMTNLLRY 240

Query: 241 HSLINGFLGVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSEL 300
           HS IN     +GK+V+K++ QGAATTCY ALHPQ  G+S +Y  D+NI+  +SQ  D +L
Sbjct: 241 HSFINTIGNAVGKYVLKSIPQGAATTCYAALHPQAKGVSGEYLMDNNISDPNSQGKDKDL 300

Query: 301 AKRLWDFTQKL 312
           AK+LW+F+ +L
Sbjct: 301 AKKLWEFSLRL 311

BLAST of IVF0002490 vs. TAIR 10
Match: AT4G23420.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 413.3 bits (1061), Expect = 1.8e-115
Identity = 208/306 (67.97%), Postives = 258/306 (84.31%), Query Frame = 0

Query: 10  SGFSASSTAEEVTQGINGNGLTAIVTGASSGIGSETARVLALRGVHVIMAVRNVATGREV 69
           SGFS+ STAEEVT G++G GLTAIVTGASSGIG ETARVLALRGVHV+MAVRN   G +V
Sbjct: 27  SGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLALRGVHVVMAVRNTGAGAKV 86

Query: 70  QKAIVKENPTAKVDTMELDLSSMASVRNFTSNFKSSGLPLNILVNNAGVMASPFLLSKDK 129
           ++ IVK+ P AKVD MEL+LSSM SVR F S +KS+GLPLN+L+NNAG+MA PF+LSKD 
Sbjct: 87  KEDIVKQVPGAKVDVMELELSSMESVRKFASEYKSAGLPLNLLINNAGIMACPFMLSKDN 146

Query: 130 LELQFATNHVGHFLLTNLLLETLKKTAKDSEREGRIVNVSSRRHQFSYREGIRFDKINDQ 189
           +ELQFATNH+GHFLLT LLL+T+K T+++S+REGRIVNVSS  H++SY EG+RFDKIND+
Sbjct: 147 IELQFATNHLGHFLLTKLLLDTMKNTSRESKREGRIVNVSSEAHRYSYPEGVRFDKINDE 206

Query: 190 SGYNGLSAYGQSKLANILHASELARQLKEEGVKITANSLHPGAIPTNLFRY-HSLINGFL 249
           S Y+ + AYGQSKL N+LHA+ELA+QLKE+GV ITANSLHPGAI TNL+ Y +S + G +
Sbjct: 207 SSYSSIRAYGQSKLCNVLHANELAKQLKEDGVNITANSLHPGAIMTNLWGYFNSYLAGAV 266

Query: 250 GVLGKHVMKNVQQGAATTCYVALHPQVNGISAQYFADSNIAKASSQANDSELAKRLWDFT 309
           G + K+++K+V QGAATTCYVAL+PQV G++ +YF+DSNIAK      D+ELAK+LWDF+
Sbjct: 267 GAVAKYMVKSVPQGAATTCYVALNPQVAGVTGEYFSDSNIAKPIELVKDTELAKKLWDFS 326

Query: 310 QKLVSS 315
            KL  S
Sbjct: 327 TKLTDS 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6RVV41.3e-11867.73Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3... [more]
A2RVM07.7e-11666.67Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
A0A078ISJ63.6e-8954.37Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
A0A078IS661.4e-8854.37Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T... [more]
Q91WL81.6e-4938.32WW domain-containing oxidoreductase OS=Mus musculus OX=10090 GN=Wwox PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7TIJ91.0e-171100.00Short-chain dehydrogenase TIC 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AUT54.3e-17098.75short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=... [more]
A0A0A0KNI09.4e-16594.98Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G141110 PE=3 SV=1[more]
A0A6J1E3K61.8e-15590.48short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36... [more]
A0A6J1I6C24.4e-15490.10short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661... [more]
Match NameE-valueIdentityDescription
KAA0042608.14.38e-221100.00short-chain dehydrogenase TIC 32 [Cucumis melo var. makuwa] >TYK06010.1 short-ch... [more]
XP_008437509.15.96e-21998.75PREDICTED: short-chain dehydrogenase TIC 32, chloroplastic-like [Cucumis melo][more]
XP_004145893.16.11e-21294.98short-chain dehydrogenase TIC 32, chloroplastic isoform X1 [Cucumis sativus] >KG... [more]
XP_023549790.11.11e-20090.79short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo... [more]
XP_022922236.19.13e-20090.48short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT4G23420.13.4e-11967.62NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23420.23.4e-11967.62NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23430.25.5e-11766.67NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G11410.11.6e-11667.52NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G23420.31.8e-11567.97NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 221..238
score: 31.98
coord: 157..173
score: 28.87
coord: 198..217
score: 27.74
coord: 107..118
score: 50.51
coord: 31..48
score: 46.81
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 163..171
score: 54.82
coord: 198..217
score: 28.03
coord: 107..118
score: 47.61
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 31..173
e-value: 1.8E-25
score: 89.6
NoneNo IPR availableGENE3D3.40.50.720coord: 25..317
e-value: 3.8E-78
score: 264.5
NoneNo IPR availablePANTHERPTHR24320RETINOL DEHYDROGENASEcoord: 2..312
NoneNo IPR availablePANTHERPTHR24320:SF230NAD(P)-BINDING ROSSMANN-FOLD PROTEINcoord: 2..312
NoneNo IPR availableCDDcd05327retinol-DH_like_SDR_c_likecoord: 29..305
e-value: 6.77254E-93
score: 275.257
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 29..288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0002490.4IVF0002490.4mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity