IVF0001950 (gene) Melon (IVF77) v1

Overview
NameIVF0001950
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionMetalloendoproteinase 2-MMP-like
Locationchr09: 2830265 .. 2831155 (-)
RNA-Seq ExpressionIVF0001950
SyntenyIVF0001950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTTCAACTCTCTCTTCAACTTTTCCTTCTAATTGCCTCTGTTGCGCCTCACGTGATCACCGCACGTGTTCGTCACACCCACATACCTTCCCATCTTAAAGGAAGTCGTATAGGTAACAACATCGAAGGAATCTACAGTGTCAAACTTTACCTCAAACGTTATGGTTACTTAATTACCAATGTAGCGAATACCGATTCTAACGACACATTCGACGTTCTCCTAGAGTCGGCTATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGTGTTTTAGATAAGGAGACATTAACCCTGATGTCTCTACCTCGATGCGGGATTTCAGATATCGTGCACGATAATAATGTCATGGATAACAACATTCAAATGAATAGTAGCAGTTTTCATTCTCATTACACATTCCTTCCGGGGAACCCGAAATGGCCGATATCAAAATACAACCTGAAGTACACATTTCTTGAGGGCTTTCCAAATGACTTCAAAGAACCGGTGATGAACGCAATGGACCAATGGGCGTTGTTTAGCTTGTTCAGATTCTCAGAAACCATAGGAGGTGAAGAGGCTGACATCACATTTAACTTTGTGAGAGGGAACCATGGAGATGGTCATCCATTCGATGGAAAAGGAGGGGTTTTGGCACATGCTTTTGGGCCATTGGATGGGAGGGTTCACTTTGATTGGGATGAGGATTGGGCAGATGGTTCCGTTGGTGGTTTTATAAATGTGGGGATGGTTGCTTTGCATGAGCTCGGGCATGTGCTTGGGCTTGCTCACAGCACCATCAGAGACGCCATTATGTGGCCCTACATGGACGTCGGTGAACAAACTAGGGGCTTACAGTTTGATGATATTGAAGGCATCCAAACTTTGTATGCTCCATGA

mRNA sequence

ATGGCTCTTCAACTCTCTCTTCAACTTTTCCTTCTAATTGCCTCTGTTGCGCCTCACGTGATCACCGCACGTGTTCGTCACACCCACATACCTTCCCATCTTAAAGGAAGTCGTATAGGTAACAACATCGAAGGAATCTACAGTGTCAAACTTTACCTCAAACGTTATGGTTACTTAATTACCAATGTAGCGAATACCGATTCTAACGACACATTCGACGTTCTCCTAGAGTCGGCTATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGTGTTTTAGATAAGGAGACATTAACCCTGATGTCTCTACCTCGATGCGGGATTTCAGATATCGTGCACGATAATAATGTCATGGATAACAACATTCAAATGAATAGTAGCAGTTTTCATTCTCATTACACATTCCTTCCGGGGAACCCGAAATGGCCGATATCAAAATACAACCTGAAGTACACATTTCTTGAGGGCTTTCCAAATGACTTCAAAGAACCGGTGATGAACGCAATGGACCAATGGGCGTTGTTTAGCTTGTTCAGATTCTCAGAAACCATAGGAGGTGAAGAGGCTGACATCACATTTAACTTTGTGAGAGGGAACCATGGAGATGGTCATCCATTCGATGGAAAAGGAGGGGTTTTGGCACATGCTTTTGGGCCATTGGATGGGAGGGTTCACTTTGATTGGGATGAGGATTGGGCAGATGGTTCCGTTGGTGGTTTTATAAATGTGGGGATGGTTGCTTTGCATGAGCTCGGGCATGTGCTTGGGCTTGCTCACAGCACCATCAGAGACGCCATTATGTGGCCCTACATGGACGTCGGTGAACAAACTAGGGGCTTACAGTTTGATGATATTGAAGGCATCCAAACTTTGTATGCTCCATGA

Coding sequence (CDS)

ATGGCTCTTCAACTCTCTCTTCAACTTTTCCTTCTAATTGCCTCTGTTGCGCCTCACGTGATCACCGCACGTGTTCGTCACACCCACATACCTTCCCATCTTAAAGGAAGTCGTATAGGTAACAACATCGAAGGAATCTACAGTGTCAAACTTTACCTCAAACGTTATGGTTACTTAATTACCAATGTAGCGAATACCGATTCTAACGACACATTCGACGTTCTCCTAGAGTCGGCTATTAAAACATTCCAAAAATACCATAGCCTCAACGTGAGTGGTGTTTTAGATAAGGAGACATTAACCCTGATGTCTCTACCTCGATGCGGGATTTCAGATATCGTGCACGATAATAATGTCATGGATAACAACATTCAAATGAATAGTAGCAGTTTTCATTCTCATTACACATTCCTTCCGGGGAACCCGAAATGGCCGATATCAAAATACAACCTGAAGTACACATTTCTTGAGGGCTTTCCAAATGACTTCAAAGAACCGGTGATGAACGCAATGGACCAATGGGCGTTGTTTAGCTTGTTCAGATTCTCAGAAACCATAGGAGGTGAAGAGGCTGACATCACATTTAACTTTGTGAGAGGGAACCATGGAGATGGTCATCCATTCGATGGAAAAGGAGGGGTTTTGGCACATGCTTTTGGGCCATTGGATGGGAGGGTTCACTTTGATTGGGATGAGGATTGGGCAGATGGTTCCGTTGGTGGTTTTATAAATGTGGGGATGGTTGCTTTGCATGAGCTCGGGCATGTGCTTGGGCTTGCTCACAGCACCATCAGAGACGCCATTATGTGGCCCTACATGGACGTCGGTGAACAAACTAGGGGCTTACAGTTTGATGATATTGAAGGCATCCAAACTTTGTATGCTCCATGA

Protein sequence

MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP
Homology
BLAST of IVF0001950 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.3e-48
Identity = 104/280 (37.14%), Postives = 159/280 (56.79%), Query Frame = 0

Query: 32  SHLKGSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNV 91
           S+  G   G N++G+Y +K Y +R+GY I    + +  D FD +L++A++ +Q   +LNV
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGY-IPETFSGNFTDDFDDILKAAVELYQTNFNLNV 104

Query: 92  SGVLDKETLTLMSLPRCGISDIVHDNNVMD----NNIQMNSSSFHSH----YTFLPGNPK 151
           +G LD  T+  + +PRCG  D+V+  ++M        ++N S  H H    YT  PG P+
Sbjct: 105 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 164

Query: 152 WPISKYNLKYTFLEGFP--NDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGN 211
           WP ++ +L Y F    P   + K     A  +W+  +   F+ +     +DIT  F  G+
Sbjct: 165 WPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGD 224

Query: 212 HGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW-ADGSVGGFINV------GMVALHELG 271
           HGDG PFDG  G LAHAF P  G+ H D DE+W   G +  F++V        VA+HE+G
Sbjct: 225 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 284

Query: 272 HVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLY 295
           H+LGL HS++ ++IM+P +  G++   L  DD+EGIQ LY
Sbjct: 285 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of IVF0001950 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.4e-48
Identity = 111/302 (36.75%), Postives = 162/302 (53.64%), Query Frame = 0

Query: 5   LSLQLFLLIASVAP-HVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLITNV 64
           +S + +  ++S+ P   + A        S L G  IG NI G+  +K Y +R+GY+ T  
Sbjct: 18  ISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYFRRFGYITT-- 77

Query: 65  ANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDNN 124
              +  D FD +L+SAI T+QK  +L V+G LD  TL  +  PRCG  D++   + M+  
Sbjct: 78  -TGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGG 137

Query: 125 IQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFL--EGFPNDFKEPVMNAMDQWALFSLFR 184
             + ++     Y+F PG P+WP  K +L Y F       ++ K     A  +WA  +   
Sbjct: 138 KILRTT---EKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLN 197

Query: 185 FSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW--ADGSV 244
           F+ +     ADI   F  G HGDG PFDG  G LAHA  P  G +H D DEDW  ++G +
Sbjct: 198 FTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEI 257

Query: 245 G-------GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQT 295
                     +++  VA+HE+GH+LGL HS++ DAIM+P +  G++   L  DDIEGIQ 
Sbjct: 258 SRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQH 313

BLAST of IVF0001950 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.0e-45
Identity = 98/278 (35.25%), Postives = 147/278 (52.88%), Query Frame = 0

Query: 36  GSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVL 95
           G   G   +G+Y +K Y + +GY+     + +  D FD +L++A++ +Q+   LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFH---------SHYTFLPGNPKWPI 155
           D+ TL  + +PRCG  D+V+  + M +  +    SF           HY+F PG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYNLKYTF--LEGFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGNHGD 215
           ++ +L Y F        + K     A  +W   +   F+       +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG--GFINVG------MVALHELGHV 275
           G PFDG    LAHAF P  G  H D +E+W     G  GFI+V        VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLY 295
           LGL HS++  +IM+P +  G +   L  DD+EG+Q LY
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of IVF0001950 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.3e-43
Identity = 107/263 (40.68%), Postives = 138/263 (52.47%), Query Frame = 0

Query: 40  GNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKET 99
           G N +G+ +VK Y    GY I N  + D  D FD  L SAIKT+QK ++LNV+G  D  T
Sbjct: 48  GQNYKGLSNVKNYFHHLGY-IPNAPHFD--DNFDDTLVSAIKTYQKNYNLNVTGKFDINT 107

Query: 100 LTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFL--E 159
           L  +  PRCG+ DI+ + N      +  S    S YTF    P+W      L Y F    
Sbjct: 108 LKQIMTPRCGVPDIIINTN------KTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEP 167

Query: 160 GFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAH 219
              + FK  +  A  +W       F ET   E A+I   F   NHGD +PFDG GG+L H
Sbjct: 168 RLDDTFKSAIARAFSKWTPVVNIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGH 227

Query: 220 AFGPLDGRVHFDWDEDW-ADGS-----VGGFINVGMVALHELGHVLGLAHSTIRDAIMWP 279
           AF P DGR HFD DE W A G      V    ++  VA+HE+GH+LGL HS+   AIM+P
Sbjct: 228 AFAPTDGRCHFDADEYWVASGDVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYP 287

Query: 280 YMDVGEQTRGLQFDDIEGIQTLY 295
            +    +   L  DDI+GI+ LY
Sbjct: 288 SIPPRTRKVNLAQDDIDGIRKLY 301

BLAST of IVF0001950 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.4e-42
Identity = 108/283 (38.16%), Postives = 148/283 (52.30%), Query Frame = 0

Query: 29  HIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHS 88
           H  S L   +IG+++ G+  +K YL R+GY+  N  +   +D FD  LESAI  +Q+   
Sbjct: 53  HDFSRLVDVQIGSHVSGVSELKRYLHRFGYV--NDGSEIFSDVFDGPLESAISLYQENLG 112

Query: 89  LNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFH--SHYTFLPGNPKW-- 148
           L ++G LD  T+TLMSLPRCG+S         D ++ +N+   H  +HYT+  G PKW  
Sbjct: 113 LPITGRLDTSTVTLMSLPRCGVS---------DTHMTINNDFLHTTAHYTYFNGKPKWNR 172

Query: 149 -----PISK-YNLKYTFLEGFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFV 208
                 ISK + L Y   E    D K     A  QW+      F E      AD+   F 
Sbjct: 173 DTLTYAISKTHKLDYLTSE----DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFY 232

Query: 209 RGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW-----ADGSVGGFINVGMVALHEL 268
            G+HGDG PFDG  G LAHAF P +GR+H D  E W       GS    +++  VA HE+
Sbjct: 233 AGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEI 292

Query: 269 GHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 297
           GH+LGL HS+   A+M+P +    +   L  DD+ G+  LY P
Sbjct: 293 GHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGP 320

BLAST of IVF0001950 vs. ExPASy TrEMBL
Match: A0A5D3BRW3 (Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00130 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.3e-172
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM
Sbjct: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 297
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 296

BLAST of IVF0001950 vs. ExPASy TrEMBL
Match: A0A1S3C931 (metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498173 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.2e-169
Identity = 292/296 (98.65%), Postives = 293/296 (98.99%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNV NTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVH+NNVM
Sbjct: 61  TNVPNTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHNNNVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSETIGGEE DITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSETIGGEEVDITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 297
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYM VGEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMHVGEQTRGLQFDDIEGIQTLYAP 296

BLAST of IVF0001950 vs. ExPASy TrEMBL
Match: A0A0A0KU02 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632080 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.2e-152
Identity = 261/296 (88.18%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQ SLQLFLLIAS+APHVITARVRHTHI SHL+GSRIGNNIEGI++VKLYLKRYGYL 
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYL- 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNV +T+SN+TFDVLLESAIKTFQKYHSLNVSGV+D+ETLTLMSLPRCGI DI+H+ NVM
Sbjct: 61  TNVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNN+QMNSSSFHSH+TF P N KWP+SKYNL+YTFL+ FPNDFKEPVMNAM+QWALFSLF
Sbjct: 121 DNNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSE +  +EADITFNFVRGNHGDG+PFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 297
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYM+ GEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYAP 295

BLAST of IVF0001950 vs. ExPASy TrEMBL
Match: A0A6J1BZK9 (metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.7e-80
Identity = 159/298 (53.36%), Postives = 199/298 (66.78%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYL- 60
           MA +      LL+ASVAP VI +   H   P HL+G R G+N+EGI +VK++L+RYGY+ 
Sbjct: 1   MAFKCLKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMR 60

Query: 61  --ITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDN 120
               N  N +S + FD  LESAIK +QK  +LN+SG LD+ETL LMS PRCG+ DIV  +
Sbjct: 61  KDNNNTVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASS 120

Query: 121 NVMDNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALF 180
           N   N   M +S   SHY F PGN KWP +KY+L YTF+  +P+++   V  A+  WA  
Sbjct: 121 N---NETMMWNS---SHYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGA 180

Query: 181 SLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADG 240
           S F F+E   GE ADI  +F RGNHGDG+PFDG+GGVLAHAF P DGR+H D DE W +G
Sbjct: 181 SQFTFAEAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEG 240

Query: 241 SVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYA 296
            V    +V  VALHELGH+LGL HS I  AIMWP++D G  T+GL  DDI+GI  LYA
Sbjct: 241 VVFNEFDVETVALHELGHILGLGHSKIEAAIMWPFLDPG-ATKGLNADDIDGIHALYA 291

BLAST of IVF0001950 vs. ExPASy TrEMBL
Match: A0A6J1BWG7 (metalloendoproteinase 5-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006148 PE=3 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 8.9e-77
Identity = 156/305 (51.15%), Postives = 201/305 (65.90%), Query Frame = 0

Query: 1   MALQLSLQ-LFLLIASVAPHVITARVRHTH------IPSHLKGSRIGNNIEGIYSVKLYL 60
           MA + SLQ LFL++A+VAPHVI AR  H H       P HL GS  GN IEGI+ +K+YL
Sbjct: 1   MASKFSLQVLFLVLATVAPHVILARDLHGHKSYPRLFPQHLAGSDKGNTIEGIHDMKIYL 60

Query: 61  KRYGYLITNVANTDSNDT-FDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISD 120
           +RYGYL  N     +ND  FD +LESA+K +QK+  LNVSG+LD ETL LMS PRCG++D
Sbjct: 61  QRYGYLTNNNNVEITNDNGFDDVLESAVKKYQKFFKLNVSGILDAETLDLMSKPRCGVAD 120

Query: 121 IVHDNNVMD---NNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMN 180
               +N  +   +N+++N     SHYT  P   +WP+SKY+L + F+  FP +F  PV  
Sbjct: 121 FFTKDNEDEQEHHNLRVNG----SHYTLFPNRQRWPVSKYHLTFGFIHQFPREFIAPVAR 180

Query: 181 AMDQWALFSLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFD 240
           A+  W   ++F FS       ADI  +F R +HGDG PFDG GG+LAHA GP +GRVHFD
Sbjct: 181 ALKSWDSNTMFSFSPAAHWPAADIVLSFERRDHGDGAPFDGLGGILAHASGPNEGRVHFD 240

Query: 241 WDEDWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEG 295
            DE+W+ G     +N+ MVALHELGHVLGL HST+++A+MWPY  +GE    L  DDI G
Sbjct: 241 GDENWSVGVAADRLNLEMVALHELGHVLGLGHSTVKEAVMWPYAGLGEGKVVLHPDDIAG 300

BLAST of IVF0001950 vs. NCBI nr
Match: KAA0037451.1 (metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01925.1 metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa])

HSP 1 Score: 609 bits (1571), Expect = 3.47e-220
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM
Sbjct: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 296
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 296

BLAST of IVF0001950 vs. NCBI nr
Match: XP_008458910.1 (PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo])

HSP 1 Score: 601 bits (1550), Expect = 5.53e-217
Identity = 292/296 (98.65%), Postives = 293/296 (98.99%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI
Sbjct: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNV NTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVH+NNVM
Sbjct: 61  TNVPNTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHNNNVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF
Sbjct: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSETIGGEE DITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSETIGGEEVDITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 296
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYM VGEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMHVGEQTRGLQFDDIEGIQTLYAP 296

BLAST of IVF0001950 vs. NCBI nr
Match: XP_004142465.1 (metalloendoproteinase 5-MMP [Cucumis sativus] >KGN52409.1 hypothetical protein Csa_009097 [Cucumis sativus])

HSP 1 Score: 544 bits (1401), Expect = 2.75e-194
Identity = 261/296 (88.18%), Postives = 282/296 (95.27%), Query Frame = 0

Query: 1   MALQLSLQLFLLIASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI 60
           MALQ SLQLFLLIAS+APHVITARVRHTHI SHL+GSRIGNNIEGI++VKLYLKRYGYL 
Sbjct: 1   MALQFSLQLFLLIASIAPHVITARVRHTHISSHLQGSRIGNNIEGIHNVKLYLKRYGYL- 60

Query: 61  TNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVM 120
           TNV +T+SN+TFDVLLESAIKTFQKYHSLNVSGV+D+ETLTLMSLPRCGI DI+H+ NVM
Sbjct: 61  TNVESTNSNNTFDVLLESAIKTFQKYHSLNVSGVIDEETLTLMSLPRCGIPDIMHNINVM 120

Query: 121 DNNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLF 180
           DNN+QMNSSSFHSH+TF P N KWP+SKYNL+YTFL+ FPNDFKEPVMNAM+QWALFSLF
Sbjct: 121 DNNVQMNSSSFHSHFTFFPDNLKWPVSKYNLRYTFLDDFPNDFKEPVMNAMEQWALFSLF 180

Query: 181 RFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240
           RFSE +  +EADITFNFVRGNHGDG+PFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG
Sbjct: 181 RFSEAVEAQEADITFNFVRGNHGDGYPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG 240

Query: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 296
           GFINVGMVALHELGHVLGLAHSTIRDAIMWPYM+ GEQTRGLQFDDIEGIQTLYAP
Sbjct: 241 GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMEAGEQTRGLQFDDIEGIQTLYAP 295

BLAST of IVF0001950 vs. NCBI nr
Match: XP_022133603.1 (metalloendoproteinase 1-like [Momordica charantia])

HSP 1 Score: 306 bits (784), Expect = 1.74e-100
Identity = 160/293 (54.61%), Postives = 200/293 (68.26%), Query Frame = 0

Query: 7   LQLFLLI-ASVAPHVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLI---TN 66
           L+LFLL+ ASVAP VI +   H   P HL+G R G+N+EGI +VK++L+RYGY+     N
Sbjct: 6   LKLFLLLLASVAPRVILSHDHHIQFPEHLQGCRKGDNVEGIQNVKMHLQRYGYMRKDNNN 65

Query: 67  VANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDN 126
             N +S + FD  LESAIK +QK  +LN+SG LD+ETL LMS PRCG+ DIV  +N   N
Sbjct: 66  TVNKESTNAFDEALESAIKKYQKNFNLNLSGALDRETLDLMSRPRCGVPDIVASSN---N 125

Query: 127 NIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMNAMDQWALFSLFRF 186
              M +SS   HY F PGN KWP +KY+L YTF+  +P+++   V  A+  WA  S F F
Sbjct: 126 ETMMWNSS---HYAFFPGNLKWPNTKYHLTYTFINNYPSNYVAAVARALATWAGASQFTF 185

Query: 187 SETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVGGF 246
           +E   GE ADI  +F RGNHGDG+PFDG+GGVLAHAF P DGR+H D DE W +G V   
Sbjct: 186 AEAFEGETADIKISFERGNHGDGNPFDGRGGVLAHAFAPSDGRLHLDADESWTEGVVFNE 245

Query: 247 INVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYA 295
            +V  VALHELGH+LGL HS I  AIMWP++D G  T+GL  DDI+GI  LYA
Sbjct: 246 FDVETVALHELGHILGLGHSKIEAAIMWPFLDPGA-TKGLNADDIDGIHALYA 291

BLAST of IVF0001950 vs. NCBI nr
Match: XP_022133609.1 (metalloendoproteinase 5-MMP-like [Momordica charantia])

HSP 1 Score: 293 bits (750), Expect = 3.57e-95
Identity = 156/305 (51.15%), Postives = 201/305 (65.90%), Query Frame = 0

Query: 1   MALQLSLQ-LFLLIASVAPHVITARVRHTH------IPSHLKGSRIGNNIEGIYSVKLYL 60
           MA + SLQ LFL++A+VAPHVI AR  H H       P HL GS  GN IEGI+ +K+YL
Sbjct: 1   MASKFSLQVLFLVLATVAPHVILARDLHGHKSYPRLFPQHLAGSDKGNTIEGIHDMKIYL 60

Query: 61  KRYGYLITNVANTDSNDT-FDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISD 120
           +RYGYL  N     +ND  FD +LESA+K +QK+  LNVSG+LD ETL LMS PRCG++D
Sbjct: 61  QRYGYLTNNNNVEITNDNGFDDVLESAVKKYQKFFKLNVSGILDAETLDLMSKPRCGVAD 120

Query: 121 IVHDNNVMD---NNIQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFLEGFPNDFKEPVMN 180
               +N  +   +N+++N S    HYT  P   +WP+SKY+L + F+  FP +F  PV  
Sbjct: 121 FFTKDNEDEQEHHNLRVNGS----HYTLFPNRQRWPVSKYHLTFGFIHQFPREFIAPVAR 180

Query: 181 AMDQWALFSLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFD 240
           A+  W   ++F FS       ADI  +F R +HGDG PFDG GG+LAHA GP +GRVHFD
Sbjct: 181 ALKSWDSNTMFSFSPAAHWPAADIVLSFERRDHGDGAPFDGLGGILAHASGPNEGRVHFD 240

Query: 241 WDEDWADGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEG 294
            DE+W+ G     +N+ MVALHELGHVLGL HST+++A+MWPY  +GE    L  DDI G
Sbjct: 241 GDENWSVGVAADRLNLEMVALHELGHVLGLGHSTVKEAVMWPYAGLGEGKVVLHPDDIAG 300

BLAST of IVF0001950 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 194.9 bits (494), Expect = 9.2e-50
Identity = 104/280 (37.14%), Postives = 159/280 (56.79%), Query Frame = 0

Query: 32  SHLKGSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNV 91
           S+  G   G N++G+Y +K Y +R+GY I    + +  D FD +L++A++ +Q   +LNV
Sbjct: 45  SNFTGCHHGQNVDGLYRIKKYFQRFGY-IPETFSGNFTDDFDDILKAAVELYQTNFNLNV 104

Query: 92  SGVLDKETLTLMSLPRCGISDIVHDNNVMD----NNIQMNSSSFHSH----YTFLPGNPK 151
           +G LD  T+  + +PRCG  D+V+  ++M        ++N S  H H    YT  PG P+
Sbjct: 105 TGELDALTIQHIVIPRCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPR 164

Query: 152 WPISKYNLKYTFLEGFP--NDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGN 211
           WP ++ +L Y F    P   + K     A  +W+  +   F+ +     +DIT  F  G+
Sbjct: 165 WPRNRRDLTYAFDPKNPLTEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGD 224

Query: 212 HGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW-ADGSVGGFINV------GMVALHELG 271
           HGDG PFDG  G LAHAF P  G+ H D DE+W   G +  F++V        VA+HE+G
Sbjct: 225 HGDGEPFDGVLGTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIG 284

Query: 272 HVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLY 295
           H+LGL HS++ ++IM+P +  G++   L  DD+EGIQ LY
Sbjct: 285 HLLGLGHSSVEESIMYPTITTGKRKVDLTNDDVEGIQYLY 323

BLAST of IVF0001950 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 192.6 bits (488), Expect = 4.6e-49
Identity = 111/302 (36.75%), Postives = 162/302 (53.64%), Query Frame = 0

Query: 5   LSLQLFLLIASVAP-HVITARVRHTHIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLITNV 64
           +S + +  ++S+ P   + A        S L G  IG NI G+  +K Y +R+GY+ T  
Sbjct: 18  ISAKFYTNVSSIPPLQFLNATQNAWETFSKLAGCHIGENINGLSKLKQYFRRFGYITT-- 77

Query: 65  ANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDNN 124
              +  D FD +L+SAI T+QK  +L V+G LD  TL  +  PRCG  D++   + M+  
Sbjct: 78  -TGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGG 137

Query: 125 IQMNSSSFHSHYTFLPGNPKWPISKYNLKYTFL--EGFPNDFKEPVMNAMDQWALFSLFR 184
             + ++     Y+F PG P+WP  K +L Y F       ++ K     A  +WA  +   
Sbjct: 138 KILRTT---EKYSFFPGKPRWPKRKRDLTYAFAPQNNLTDEVKRVFSRAFTRWAEVTPLN 197

Query: 185 FSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW--ADGSV 244
           F+ +     ADI   F  G HGDG PFDG  G LAHA  P  G +H D DEDW  ++G +
Sbjct: 198 FTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEI 257

Query: 245 G-------GFINVGMVALHELGHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQT 295
                     +++  VA+HE+GH+LGL HS++ DAIM+P +  G++   L  DDIEGIQ 
Sbjct: 258 SRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQH 313

BLAST of IVF0001950 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 185.3 bits (469), Expect = 7.3e-47
Identity = 98/278 (35.25%), Postives = 147/278 (52.88%), Query Frame = 0

Query: 36  GSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVL 95
           G   G   +G+Y +K Y + +GY+     + +  D FD +L++A++ +Q+   LNV+GVL
Sbjct: 51  GCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVL 110

Query: 96  DKETLTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFH---------SHYTFLPGNPKWPI 155
           D+ TL  + +PRCG  D+V+  + M +  +    SF           HY+F PG P+WP 
Sbjct: 111 DELTLKHVVIPRCGNPDVVNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPR 170

Query: 156 SKYNLKYTF--LEGFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGNHGD 215
           ++ +L Y F        + K     A  +W   +   F+       +DI+  F  G HGD
Sbjct: 171 NRRDLTYAFDPRNALTEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGD 230

Query: 216 GHPFDGKGGVLAHAFGPLDGRVHFDWDEDWADGSVG--GFINVG------MVALHELGHV 275
           G PFDG    LAHAF P  G  H D +E+W     G  GFI+V        VA+HE+GH+
Sbjct: 231 GEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHL 290

Query: 276 LGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLY 295
           LGL HS++  +IM+P +  G +   L  DD+EG+Q LY
Sbjct: 291 LGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLY 328

BLAST of IVF0001950 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 174.9 bits (442), Expect = 9.8e-44
Identity = 108/283 (38.16%), Postives = 148/283 (52.30%), Query Frame = 0

Query: 29  HIPSHLKGSRIGNNIEGIYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHS 88
           H  S L   +IG+++ G+  +K YL R+GY+  N  +   +D FD  LESAI  +Q+   
Sbjct: 53  HDFSRLVDVQIGSHVSGVSELKRYLHRFGYV--NDGSEIFSDVFDGPLESAISLYQENLG 112

Query: 89  LNVSGVLDKETLTLMSLPRCGISDIVHDNNVMDNNIQMNSSSFH--SHYTFLPGNPKW-- 148
           L ++G LD  T+TLMSLPRCG+S         D ++ +N+   H  +HYT+  G PKW  
Sbjct: 113 LPITGRLDTSTVTLMSLPRCGVS---------DTHMTINNDFLHTTAHYTYFNGKPKWNR 172

Query: 149 -----PISK-YNLKYTFLEGFPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFV 208
                 ISK + L Y   E    D K     A  QW+      F E      AD+   F 
Sbjct: 173 DTLTYAISKTHKLDYLTSE----DVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFY 232

Query: 209 RGNHGDGHPFDGKGGVLAHAFGPLDGRVHFDWDEDW-----ADGSVGGFINVGMVALHEL 268
            G+HGDG PFDG  G LAHAF P +GR+H D  E W       GS    +++  VA HE+
Sbjct: 233 AGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEI 292

Query: 269 GHVLGLAHSTIRDAIMWPYMDVGEQTRGLQFDDIEGIQTLYAP 297
           GH+LGL HS+   A+M+P +    +   L  DD+ G+  LY P
Sbjct: 293 GHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGP 320

BLAST of IVF0001950 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 157.9 bits (398), Expect = 1.2e-38
Identity = 93/262 (35.50%), Postives = 135/262 (51.53%), Query Frame = 0

Query: 46  IYSVKLYLKRYGYLITNVANTDSNDTFDVLLESAIKTFQKYHSLNVSGVLDKETLTLMSL 105
           I  +K +L++YGYL  N       ++ DV  E A+  +QK   L ++G  D +TL+ + L
Sbjct: 50  IPEIKRHLQQYGYLPQN------KESDDVSFEQALVRYQKNLGLPITGKPDSDTLSQILL 109

Query: 106 PRCGISDIVHDNNVMDNNIQMNSSSFHS--HYTFLPGNPKWPIS-KYNLKYTFLEG---- 165
           PRCG  D          +++  ++ FH+   Y + PG P+W       L Y F +     
Sbjct: 110 PRCGFPD----------DVEPKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTP 169

Query: 166 --FPNDFKEPVMNAMDQWALFSLFRFSETIGGEEADITFNFVRGNHGDGHPFDGKGGVLA 225
              P D +     A  +WA      F ET     ADI   F  G+HGDG PFDG  GVLA
Sbjct: 170 YLAPTDIRRVFRRAFGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLA 229

Query: 226 HAFGPLDGRVHFDWDEDWA----DGSVGGFINVGMVALHELGHVLGLAHSTIRDAIMWPY 285
           H F P +GR+H D  E WA    +      +++  VA+HE+GHVLGL HS+++DA M+P 
Sbjct: 230 HTFSPENGRLHLDKAETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPT 289

Query: 286 MDVGEQTRGLQFDDIEGIQTLY 295
           +    +   L  DD+ G+Q+LY
Sbjct: 290 LKPRSKKVNLNMDDVVGVQSLY 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O045291.3e-4837.14Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ56.4e-4836.75Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
Q5XF511.0e-4535.25Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
P291361.3e-4340.68Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
O235071.4e-4238.16Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BRW34.3e-172100.00Metalloendoproteinase 2-MMP-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C9311.2e-16998.65metalloendoproteinase 2-MMP-like OS=Cucumis melo OX=3656 GN=LOC103498173 PE=3 SV... [more]
A0A0A0KU022.2e-15288.18ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G632080 PE=3 S... [more]
A0A6J1BZK91.7e-8053.36metalloendoproteinase 1-like OS=Momordica charantia OX=3673 GN=LOC111006143 PE=3... [more]
A0A6J1BWG78.9e-7751.15metalloendoproteinase 5-MMP-like OS=Momordica charantia OX=3673 GN=LOC111006148 ... [more]
Match NameE-valueIdentityDescription
KAA0037451.13.47e-220100.00metalloendoproteinase 2-MMP-like [Cucumis melo var. makuwa] >TYK01925.1 metalloe... [more]
XP_008458910.15.53e-21798.65PREDICTED: metalloendoproteinase 2-MMP-like [Cucumis melo][more]
XP_004142465.12.75e-19488.18metalloendoproteinase 5-MMP [Cucumis sativus] >KGN52409.1 hypothetical protein C... [more]
XP_022133603.11.74e-10054.61metalloendoproteinase 1-like [Momordica charantia][more]
XP_022133609.13.57e-9551.15metalloendoproteinase 5-MMP-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G70170.19.2e-5037.14matrix metalloproteinase [more]
AT1G59970.14.6e-4936.75Matrixin family protein [more]
AT1G24140.17.3e-4735.25Matrixin family protein [more]
AT4G16640.19.8e-4438.16Matrixin family protein [more]
AT2G45040.11.2e-3835.50Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 103..116
score: 42.51
coord: 282..295
score: 57.06
coord: 191..219
score: 61.1
coord: 248..273
score: 56.29
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 140..296
e-value: 4.5E-28
score: 109.3
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 46..103
e-value: 1.9E-12
score: 47.2
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 161..295
e-value: 5.2E-39
score: 133.6
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 36..296
e-value: 1.0E-68
score: 233.4
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 26..295
NoneNo IPR availablePANTHERPTHR10201:SF272METALLOENDOPROTEINASE 2-MMP-LIKEcoord: 26..295
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 136..294
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 143..294
e-value: 7.18243E-52
score: 165.84
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 40..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0001950.2IVF0001950.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity