IVF0001740 (gene) Melon (IVF77) v1

Overview
NameIVF0001740
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
DescriptionAMP-binding domain-containing protein
Locationchr03: 25590163 .. 25592277 (-)
RNA-Seq ExpressionIVF0001740
SyntenyIVF0001740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTATACGAGAATTTTGATCCATTATTCCCGGAGCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCGAGCCTTCCAGCGTTCGGGTCGAAGCCAGCGTTCATCTGGACCGAAGATGGCACCGTTGAGGCCATAAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGATACCGTTCAGTTGATCACCGAACAGTTGCTCCGGCAGTTGCGGCGGCGGGATACGGTGGTTGTATTGTGCTCGGCGGGGCTGGACTTTGTCCAGCTAATATATGGTTGTCAAAGAGCTGGGCTAGTGAGTGTCCCCATCAGCCCCCCCGACGTTTTTTCTGAGAATGAAAATTGTCACCATCTTGGTAGAGCTCTATCCCAGACTAAGCCCCGTGCCGCCATAGCTCACCAATCTTACATCACTACCGTTTTTCGGTACGTTTTCAACGAACCTTAGTTTTTATTTGTTCATTTATTTAATACTCAAATGCATCCTAATGTGCATTTATTTGTGTGTATATATATATATATAGGTACATCTCCTCATCTCCGGCCGACGAGAAGTTGGCTTTGTTATTGAAGAGCGTGCAATGGATTTCAATGGAGAGTTTAAAAGAACCCCACAAAGAATCAGAGCTCAATCAACACAAACACCATTCCTTTTCTCATCATTCTTCCTCGTACTCCGGATGTAATCCAGACGATCCTTATTTAATCCAATACACATCCGGCGCCACCGCAATTTCCAAGCCAGTGGTGATTACCGCCGGCGCCGCGGCCCACAACGTCCGTGCGGCAAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCATGGCTCCCACAGTTCCACGACTGTGGCCTTATGTTCCTTCTCTTAACCGTCATCACAGGGGCCACGTGTGTACTAACCTCACCCATATCATTCGTCACCCAACCAATCACGTGGCTCCACCTCATCACAGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGGGTAATTATTCAAGTGGGCGGGCGGGTAATTTGGATTTGCGGAGCTTGAGGAATTTGATTTTGATCAATGAACCGATTTACCGGTCTACGGTGGAGGAGTTTGTGGATGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGTGTTTCTCCGTCGTATGGGTTGGCGGAGAATTGTACTTTTGTTTCGACGGCGTGGTGTGGCGGTGGTGGGTTTCCGGCGATGCCAAGTTATCGGAAGTTGTTGCCAAGTGGGAGGTTGAGGGATGGGATGTGTAAGGAGATTGAGGTGGTGGTTGTTAATGAAGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAAATTTGGATCTCCTCGCCCAGCAATGCCTTAGGTAATTTTCTATTAAACTATAAATGTGGAATTCTTACCCTTACAAAAAATTTCAAAAATTAAAAACATTTGGTTTTTTACGAATTGATACTTTCAAAAGAAAAGCTCTCACAAAAGTTCTCTTTCAGGTTATCTTGGCCATCCATCTCTAACCGAAGAGACATTCCACCGTAAACTCAAGAACAAAGCAAGCCTTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGTTCCGACAGGTTCCTCTTCGTCATCGGCCGGTGTTCGGACGTAATCAAATTCAACGACAACAACCAACAAATCCATCCGCATTACATCGAATCCACATCTTACAAAAATTTCTCCGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCATCGGGGACAGTCGCCTTAGTTGCCGAGATGCAGAGAGACGACAGAAACGACGCCGAATTGCTGAGAAAAATATGCGAAGAAATCAGAAAAGCGGTGTTGATTGAAGAGGGAATTGAATTGGGGGTTGTGGTTTTGGTGAAGAGGGGAAATGTGTCCAAAACGACGTCGGGAAAAGTAAAGAGATGGGTGGTGAAGGAGAAGCTCGGCGGCGGGGGATTGGAGGTTTTGATGGCCGTCGAATTTGGAAAAGACTGTGAGGGTTTAAAGGATTTAGAAAGAAAAGGAGAGTTTGGAACTCGTCCTGTTCTGCTCTCCATGCTTTAA

mRNA sequence

ATGTTATACGAGAATTTTGATCCATTATTCCCGGAGCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCGAGCCTTCCAGCGTTCGGGTCGAAGCCAGCGTTCATCTGGACCGAAGATGGCACCGTTGAGGCCATAAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGATACCGTTCAGTTGATCACCGAACAGTTGCTCCGGCAGTTGCGGCGGCGGGATACGGTGGTTGTATTGTGCTCGGCGGGGCTGGACTTTGTCCAGCTAATATATGGTTGTCAAAGAGCTGGGCTAGTGAGTGTCCCCATCAGCCCCCCCGACGTTTTTTCTGAGAATGAAAATTGTCACCATCTTGGTAGAGCTCTATCCCAGACTAAGCCCCGTGCCGCCATAGCTCACCAATCTTACATCACTACCGTTTTTCGGTACATCTCCTCATCTCCGGCCGACGAGAAGTTGGCTTTGTTATTGAAGAGCGTGCAATGGATTTCAATGGAGAGTTTAAAAGAACCCCACAAAGAATCAGAGCTCAATCAACACAAACACCATTCCTTTTCTCATCATTCTTCCTCGTACTCCGGATGTAATCCAGACGATCCTTATTTAATCCAATACACATCCGGCGCCACCGCAATTTCCAAGCCAGTGGTGATTACCGCCGGCGCCGCGGCCCACAACGTCCGTGCGGCAAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCATGGCTCCCACAGTTCCACGACTGTGGCCTTATGTTCCTTCTCTTAACCGTCATCACAGGGGCCACGTGTGTACTAACCTCACCCATATCATTCGTCACCCAACCAATCACGTGGCTCCACCTCATCACAGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGGGTAATTATTCAAGTGGGCGGGCGGGTAATTTGGATTTGCGGAGCTTGAGGAATTTGATTTTGATCAATGAACCGATTTACCGGTCTACGGTGGAGGAGTTTGTGGATGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGTGTTTCTCCGTCGTATGGGTTGGCGGAGAATTGTACTTTTGTTTCGACGGCGTGGTGTGGCGGTGGTGGGTTTCCGGCGATGCCAAGTTATCGGAAGTTGTTGCCAAGTGGGAGGTTGAGGGATGGGATGTGTAAGGAGATTGAGGTGGTGGTTGTTAATGAAGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAAATTTGGATCTCCTCGCCCAGCAATGCCTTAGGTTATCTTGGCCATCCATCTCTAACCGAAGAGACATTCCACCGTAAACTCAAGAACAAAGCAAGCCTTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGTTCCGACAGGTTCCTCTTCGTCATCGGCCGGTGTTCGGACGTAATCAAATTCAACGACAACAACCAACAAATCCATCCGCATTACATCGAATCCACATCTTACAAAAATTTCTCCGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCATCGGGGACAGTCGCCTTAGTTGCCGAGATGCAGAGAGACGACAGAAACGACGCCGAATTGCTGAGAAAAATATGCGAAGAAATCAGAAAAGCGGTGTTGATTGAAGAGGGAATTGAATTGGGGGTTGTGGTTTTGGTGAAGAGGGGAAATGTGTCCAAAACGACGTCGGGAAAAGTAAAGAGATGGGTGGTGAAGGAGAAGCTCGGCGGCGGGGGATTGGAGGTTTTGATGGCCGTCGAATTTGGAAAAGACTGTGAGGGTTTAAAGGATTTAGAAAGAAAAGGAGAGTTTGGAACTCGTCCTGTTCTGCTCTCCATGCTTTAA

Coding sequence (CDS)

ATGTTATACGAGAATTTTGATCCATTATTCCCGGAGCAGCCGGTGGTCGACCGGTATCTCCCGGTTTGGGCGAGCCTTCCAGCGTTCGGGTCGAAGCCAGCGTTCATCTGGACCGAAGATGGCACCGTTGAGGCCATAAATGAAGGCTCATTTCTGACATACCGGCAACTTCACGATACCGTTCAGTTGATCACCGAACAGTTGCTCCGGCAGTTGCGGCGGCGGGATACGGTGGTTGTATTGTGCTCGGCGGGGCTGGACTTTGTCCAGCTAATATATGGTTGTCAAAGAGCTGGGCTAGTGAGTGTCCCCATCAGCCCCCCCGACGTTTTTTCTGAGAATGAAAATTGTCACCATCTTGGTAGAGCTCTATCCCAGACTAAGCCCCGTGCCGCCATAGCTCACCAATCTTACATCACTACCGTTTTTCGGTACATCTCCTCATCTCCGGCCGACGAGAAGTTGGCTTTGTTATTGAAGAGCGTGCAATGGATTTCAATGGAGAGTTTAAAAGAACCCCACAAAGAATCAGAGCTCAATCAACACAAACACCATTCCTTTTCTCATCATTCTTCCTCGTACTCCGGATGTAATCCAGACGATCCTTATTTAATCCAATACACATCCGGCGCCACCGCAATTTCCAAGCCAGTGGTGATTACCGCCGGCGCCGCGGCCCACAACGTCCGTGCGGCAAGAAAAGCCTACGACTTAAACCCAAACGACGTCATCGTTTCATGGCTCCCACAGTTCCACGACTGTGGCCTTATGTTCCTTCTCTTAACCGTCATCACAGGGGCCACGTGTGTACTAACCTCACCCATATCATTCGTCACCCAACCAATCACGTGGCTCCACCTCATCACAGCTTTCAAAGCCACGTGCACTCCCGTGCCGTCTTTCACTCTCCCACTCGTACTCAAGCGGGTGGGTAATTATTCAAGTGGGCGGGCGGGTAATTTGGATTTGCGGAGCTTGAGGAATTTGATTTTGATCAATGAACCGATTTACCGGTCTACGGTGGAGGAGTTTGTGGATGTGTTTAAGGCGGTTGGGTTGGACCCGGGTTGTGTTTCTCCGTCGTATGGGTTGGCGGAGAATTGTACTTTTGTTTCGACGGCGTGGTGTGGCGGTGGTGGGTTTCCGGCGATGCCAAGTTATCGGAAGTTGTTGCCAAGTGGGAGGTTGAGGGATGGGATGTGTAAGGAGATTGAGGTGGTGGTTGTTAATGAAGAAACTGGGGAGGTGGTTGAGGATGGCGTTGAAGGAGAAATTTGGATCTCCTCGCCCAGCAATGCCTTAGGTTATCTTGGCCATCCATCTCTAACCGAAGAGACATTCCACCGTAAACTCAAGAACAAAGCAAGCCTTAATTTCGTCCGCACCGGCGACAGAGGAGTAATTAAAGGTTCCGACAGGTTCCTCTTCGTCATCGGCCGGTGTTCGGACGTAATCAAATTCAACGACAACAACCAACAAATCCATCCGCATTACATCGAATCCACATCTTACAAAAATTTCTCCGCCTATCTTCGAGGCGGCTGCATCGCCGCCGTCAAGATATCATCGGGGACAGTCGCCTTAGTTGCCGAGATGCAGAGAGACGACAGAAACGACGCCGAATTGCTGAGAAAAATATGCGAAGAAATCAGAAAAGCGGTGTTGATTGAAGAGGGAATTGAATTGGGGGTTGTGGTTTTGGTGAAGAGGGGAAATGTGTCCAAAACGACGTCGGGAAAAGTAAAGAGATGGGTGGTGAAGGAGAAGCTCGGCGGCGGGGGATTGGAGGTTTTGATGGCCGTCGAATTTGGAAAAGACTGTGAGGGTTTAAAGGATTTAGAAAGAAAAGGAGAGTTTGGAACTCGTCCTGTTCTGCTCTCCATGCTTTAA

Protein sequence

MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML
Homology
BLAST of IVF0001740 vs. ExPASy Swiss-Prot
Match: P9WQ44 (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD25 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-42
Identity = 154/565 (27.26%), Postives = 243/565 (43.01%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVF 111
           LT+ QL+     +  QL       D  ++L    LD+V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 112 SENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK 171
           + +E    +    + T P   +   S +  V  Y+   P     A++   V  + +++  
Sbjct: 101 AHDERTVSV---FADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVI--EVDRLDLDA-- 160

Query: 172 EPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRA 231
            P   S    H H              PD  YL QYTSG+T     V+++      N   
Sbjct: 161 RPSSGSRSAAHGH--------------PDILYL-QYTSGSTRTPAGVMVSNKNLFANFEQ 220

Query: 232 ARKAY------DLNPNDVIVSWLPQFHDCGLMF-LLLTVITGATCVLTSPISFVTQPITW 291
              +Y         P   +VSWLP +HD G +  L+L ++ G   VLTSPI F+ +P  W
Sbjct: 221 IMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFLQRPARW 280

Query: 292 LHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEF 351
           + ++ +     T  P+F   L  ++  +        LDL  +  ++  +E +   T++ F
Sbjct: 281 IQMLASNTLAFTAAPNFAFDLASRKTKDED---MEGLDLGGVHGILNGSERVQPVTLKRF 340

Query: 352 VDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEI 411
           +D F    LDP  + PSYG+AE   +V+T   G       P         +L DG  +  
Sbjct: 341 IDRFAPFNLDPKAIRPSYGMAEATVYVATRKAG------QPPKIVQFDPQKLPDGQAERT 400

Query: 412 E-----------------VVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETF 471
           E                 V +V+ +TG     G  GEIW+   + A+GY   P  TE TF
Sbjct: 401 ESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYWQKPEATERTF 460

Query: 472 HRKLKNKASLN----FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTS 531
              + N +       ++RTGD G +  S+  LF++GR  D++     N    P  IE+T 
Sbjct: 461 SATIVNPSEGTPAGPWLRTGDSGFL--SEGELFIMGRIKDLLIVYGRNHS--PDDIEAT- 520

Query: 532 YKNFSAYLRGGCIAAVKISSGTVALVA--EMQRDDRND---AELLRKICEEIRKAVLIEE 584
                    G C A      G   LVA  E+++ D +D   AE L  +  E+  A+    
Sbjct: 521 ---IQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSH 566

BLAST of IVF0001740 vs. ExPASy Swiss-Prot
Match: P9WQ45 (Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD25 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-42
Identity = 154/565 (27.26%), Postives = 243/565 (43.01%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVF 111
           LT+ QL+     +  QL       D  ++L    LD+V       +AG+V+VP+S P   
Sbjct: 41  LTWSQLYRRTLNLAAQLREHGSTGDRALILAPQSLDYVVSFIASLQAGIVAVPLSIPQGG 100

Query: 112 SENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK 171
           + +E    +    + T P   +   S +  V  Y+   P     A++   V  + +++  
Sbjct: 101 AHDERTVSV---FADTAPAIVLTASSVVDNVVEYVQPQPGQNAPAVI--EVDRLDLDA-- 160

Query: 172 EPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRA 231
            P   S    H H              PD  YL QYTSG+T     V+++      N   
Sbjct: 161 RPSSGSRSAAHGH--------------PDILYL-QYTSGSTRTPAGVMVSNKNLFANFEQ 220

Query: 232 ARKAY------DLNPNDVIVSWLPQFHDCGLMF-LLLTVITGATCVLTSPISFVTQPITW 291
              +Y         P   +VSWLP +HD G +  L+L ++ G   VLTSPI F+ +P  W
Sbjct: 221 IMTSYYGVYGKVAPPGSTVVSWLPFYHDMGFVLGLILPILAGIPAVLTSPIGFLQRPARW 280

Query: 292 LHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEF 351
           + ++ +     T  P+F   L  ++  +        LDL  +  ++  +E +   T++ F
Sbjct: 281 IQMLASNTLAFTAAPNFAFDLASRKTKDED---MEGLDLGGVHGILNGSERVQPVTLKRF 340

Query: 352 VDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEI 411
           +D F    LDP  + PSYG+AE   +V+T   G       P         +L DG  +  
Sbjct: 341 IDRFAPFNLDPKAIRPSYGMAEATVYVATRKAG------QPPKIVQFDPQKLPDGQAERT 400

Query: 412 E-----------------VVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETF 471
           E                 V +V+ +TG     G  GEIW+   + A+GY   P  TE TF
Sbjct: 401 ESDGGTPLVSYGIVDTQLVRIVDPDTGIERPAGTIGEIWVHGDNVAIGYWQKPEATERTF 460

Query: 472 HRKLKNKASLN----FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTS 531
              + N +       ++RTGD G +  S+  LF++GR  D++     N    P  IE+T 
Sbjct: 461 SATIVNPSEGTPAGPWLRTGDSGFL--SEGELFIMGRIKDLLIVYGRNHS--PDDIEAT- 520

Query: 532 YKNFSAYLRGGCIAAVKISSGTVALVA--EMQRDDRND---AELLRKICEEIRKAVLIEE 584
                    G C A      G   LVA  E+++ D +D   AE L  +  E+  A+    
Sbjct: 521 ---IQTISPGRCAAIAVSEHGAEKLVAIIELKKKDESDDEAAERLGFVKREVTSAISKSH 566

BLAST of IVF0001740 vs. ExPASy Swiss-Prot
Match: B2HIL4 (4-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=fadD29 PE=1 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 1.9e-41
Identity = 158/571 (27.67%), Postives = 254/571 (44.48%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVF 111
           LT+ Q++   +++ E+L       D V VL   GL+++    G   AGL++VP+  P   
Sbjct: 79  LTWWQIYRRAKIVAEELRGYGASGDRVAVLAPQGLEYIIAFLGVLEAGLIAVPLPVPQFG 138

Query: 112 SENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK 171
             +E    +  AL  + P   +     I  V +Y   + A +    ++ +V  + ++S +
Sbjct: 139 IHDE---RISAALQDSTPSVILTTSPVIDEVTKYAPHARAGQGGTPIVVAVDLLDLDSAR 198

Query: 172 EPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRA 231
           E               +H S++Y          +QYTSG+T     VV++      N   
Sbjct: 199 ELDLTPP---------AHSSTAY----------LQYTSGSTRSPAGVVLSHKNVITNCVQ 258

Query: 232 ARKAYDLNPNDV---IVSWLPQFHDCGLMF-LLLTVITGATCVLTSPISFVTQPITWLHL 291
               Y      V    VSWLP +HD GLM  ++L +I   T VL +P++F+ +P  W+ L
Sbjct: 259 LMSDYLGETEKVPSTAVSWLPFYHDMGLMLGIILPMINQDTAVLLNPMAFLQRPARWMQL 318

Query: 292 ITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDV 351
           +  F+   +  P+F   L ++R    S      LDL  +R +    E +  +T++ F+D 
Sbjct: 319 MGKFRGQISCAPNFGFELAVRRT---SDEDMAGLDLGHVRGIGSGAERVNPATLQRFIDR 378

Query: 352 FKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRD--------- 411
           F    L    + PSYGLAE   FV+TA  G         Y+  L  GR+           
Sbjct: 379 FAPFNLRDTAIRPSYGLAEATVFVATAEPGRPPRSVNFDYQS-LSVGRVERCANEADDGA 438

Query: 412 -----GMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKN 471
                G     EV +V+ E       G  GEIW+   + A+GY  +P LTE TF  KL +
Sbjct: 439 KLVSYGSSWTSEVRIVDPEARTECPAGTVGEIWVQGDNVAMGYWRNPQLTERTFDAKLTD 498

Query: 472 KA---SLN-FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSA 531
            +   S+  ++RTGD GV+   +  LF+ GR  D++  + +N   +P  IEST  +    
Sbjct: 499 PSPGTSIGPWLRTGDLGVMFEGE--LFITGRIKDLLIVDGSNH--YPDDIESTIQE---- 558

Query: 532 YLRGGCIAAVKISSG------TVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELG 591
            + GG + A+ +         T+   A      +   + LR +  EI  A+     + + 
Sbjct: 559 -ITGGRVVAIAVPDADGEKLVTIVEFASWGHSGQEAIDKLRSVKREITSAISRAHRVRVA 614

Query: 592 VVVLVKRGNVSKTTSGKVKRWVVKEKLGGGG 595
            VVLV  G++  TTSGKV+R    E+    G
Sbjct: 619 DVVLVATGSIPVTTSGKVRRSSCAERYRNDG 614

BLAST of IVF0001740 vs. ExPASy Swiss-Prot
Match: P9WQ58 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=fadD28 PE=3 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-40
Identity = 158/577 (27.38%), Postives = 256/577 (44.37%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVF 111
           LT+ QL+     + ++L R     D VV+    GL++V    G  +AG ++VP+S P   
Sbjct: 39  LTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFLGALQAGRIAVPLSVPQGG 98

Query: 112 SENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK 171
             +E    +   LS + P A +   S +  V ++++  P +        S+  + +  L 
Sbjct: 99  VTDERSDSV---LSDSSPVAILTTSSAVDDVVQHVARRPGESP-----PSIIEVDLLDLD 158

Query: 172 EPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRA 231
            P      N +      + S++Y          +QYTSG+T     VV++      N   
Sbjct: 159 AP------NGYTFKEDEYPSTAY----------LQYTSGSTRTPAGVVMSHQNVRVNFEQ 218

Query: 232 ARKAYDLN------PNDVIVSWLPQFHDCGLMF-LLLTVITGATCVLTSPISFVTQPITW 291
               Y  +      PN  +VSWLP +HD GL+  +   ++ G   VLTSP+SF+ +P  W
Sbjct: 219 LMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAVLTSPVSFLQRPARW 278

Query: 292 LHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEF 351
           +HL+ +     +  P+F   L  +R  +     AG  DL ++  ++  +E +  +T++ F
Sbjct: 279 MHLMASDFHAFSAAPNFAFELAARRTTD--DDMAGR-DLGNILTILSGSERVQAATIKRF 338

Query: 352 VDVFKAVGLDPGCVSPSYGLAENCTFVSTAW----------------------CGGGGFP 411
            D F    L    + PSYGLAE   +V+T+                       C GGG  
Sbjct: 339 ADRFARFNLQERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAGHAKPCAGGGAT 398

Query: 412 AMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSL 471
           ++ SY  +LP   +         V +V+ +T     DG  GEIW+   + A GY   P  
Sbjct: 399 SLISY--MLPRSPI---------VRIVDSDTCIECPDGTVGEIWVHGDNVANGYWQKPDE 458

Query: 472 TEETFHRKLKNKASLN----FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHY 531
           +E TF  K+   +       ++RTGD G +  +D  +F+IGR  D++     N    P  
Sbjct: 459 SERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLLIVYGRNHS--PDD 518

Query: 532 IESTSYKNFSAYLRGGCIA-AVKISSGTVALVAEMQRDDRNDAE-----LLRKICEEIRK 590
           IE+T         RG C A +V     T  LVA ++   R D++      L  I  E+  
Sbjct: 519 IEAT----IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTS 569

BLAST of IVF0001740 vs. ExPASy Swiss-Prot
Match: P9WQ59 (Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=fadD28 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-40
Identity = 158/577 (27.38%), Postives = 256/577 (44.37%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVF 111
           LT+ QL+     + ++L R     D VV+    GL++V    G  +AG ++VP+S P   
Sbjct: 39  LTWSQLYRRTLNVAQELSRCGSTGDRVVISAPQGLEYVVAFLGALQAGRIAVPLSVPQGG 98

Query: 112 SENENCHHLGRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK 171
             +E    +   LS + P A +   S +  V ++++  P +        S+  + +  L 
Sbjct: 99  VTDERSDSV---LSDSSPVAILTTSSAVDDVVQHVARRPGESP-----PSIIEVDLLDLD 158

Query: 172 EPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRA 231
            P      N +      + S++Y          +QYTSG+T     VV++      N   
Sbjct: 159 AP------NGYTFKEDEYPSTAY----------LQYTSGSTRTPAGVVMSHQNVRVNFEQ 218

Query: 232 ARKAYDLN------PNDVIVSWLPQFHDCGLMF-LLLTVITGATCVLTSPISFVTQPITW 291
               Y  +      PN  +VSWLP +HD GL+  +   ++ G   VLTSP+SF+ +P  W
Sbjct: 219 LMSGYFADTDGIPPPNSALVSWLPFYHDMGLVIGICAPILGGYPAVLTSPVSFLQRPARW 278

Query: 292 LHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEF 351
           +HL+ +     +  P+F   L  +R  +     AG  DL ++  ++  +E +  +T++ F
Sbjct: 279 MHLMASDFHAFSAAPNFAFELAARRTTD--DDMAGR-DLGNILTILSGSERVQAATIKRF 338

Query: 352 VDVFKAVGLDPGCVSPSYGLAENCTFVSTAW----------------------CGGGGFP 411
            D F    L    + PSYGLAE   +V+T+                       C GGG  
Sbjct: 339 ADRFARFNLQERVIRPSYGLAEATVYVATSKPGQPPETVDFDTESLSAGHAKPCAGGGAT 398

Query: 412 AMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSL 471
           ++ SY  +LP   +         V +V+ +T     DG  GEIW+   + A GY   P  
Sbjct: 399 SLISY--MLPRSPI---------VRIVDSDTCIECPDGTVGEIWVHGDNVANGYWQKPDE 458

Query: 472 TEETFHRKLKNKASLN----FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHY 531
           +E TF  K+   +       ++RTGD G +  +D  +F+IGR  D++     N    P  
Sbjct: 459 SERTFGGKIVTPSPGTPEGPWLRTGDSGFV--TDGKMFIIGRIKDLLIVYGRNHS--PDD 518

Query: 532 IESTSYKNFSAYLRGGCIA-AVKISSGTVALVAEMQRDDRNDAE-----LLRKICEEIRK 590
           IE+T         RG C A +V     T  LVA ++   R D++      L  I  E+  
Sbjct: 519 IEAT----IQEITRGRCAAISVPGDRSTEKLVAIIELKKRGDSDQDAMARLGAIKREVTS 569

BLAST of IVF0001740 vs. ExPASy TrEMBL
Match: A0A0A0LL23 (AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690 PE=4 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 581/629 (92.37%), Postives = 604/629 (96.03%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           MLYENFDPLFPEQPVVDRYLPVWASLPAF SKPAFIW+EDGT +AINE SFLTYRQLHD+
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELN 180
            RALSQTKPRAAIAHQSYI TVFRYISSS AD+KLALLLK V+WISMESLK+PHKESELN
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 181 QHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNP 240
           QHKH S  +HSSSYSGCNPDDPYLIQYTSGATAISK VVITAGAAAHNVRAARKAYDLNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 241 NDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300
           ND+IVSWLPQ+HDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSP 360
           FTLPLVLKRVG YSSGRAGNLDLRSLRNLILINEPIYRSTVEEFV+VFK VGLDPGCVSP
Sbjct: 301 FTLPLVLKRVG-YSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSP 360

Query: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420
           SYGLAENCTFVSTAWCGGGGFPAMPSYR+LLPSGRLRDGMCKEIEVVVVNEETGEVVEDG
Sbjct: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420

Query: 421 VEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGR 480
           VEGEIW+SSPSNALGYLGHPS+TEETFH KLKNK+SLNFVRTGDRGVIKGSDRFLFVIGR
Sbjct: 421 VEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIGR 480

Query: 481 CSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDA 540
           CSDVIK N+NNQQIHPHYIESTSY NFSAYLRGGCIAAVK+ SGT+ALVAEMQRDDR+DA
Sbjct: 481 CSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKV-SGTIALVAEMQRDDRHDA 540

Query: 541 ELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMA 600
           ELLRKICE+IRKAVLIEEGIELG+VVLVKRGNVSKTTSGKVKRWVVKEKL GGGL VLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VEFGKDCEGLKDLERKGEFGTRPVLLSML 630
            EFGK+C    DL+RKGEF TRPVL+SML
Sbjct: 601 FEFGKNC---VDLKRKGEFETRPVLISML 623

BLAST of IVF0001740 vs. ExPASy TrEMBL
Match: A0A5A7VDG2 (Long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005700 PE=4 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 1.0e-279
Identity = 486/488 (99.59%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 142 VFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD 201
           ++RYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD
Sbjct: 11  IYRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD 70

Query: 202 PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL 261
           PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL
Sbjct: 71  PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL 130

Query: 262 TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL 321
           TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL
Sbjct: 131 TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL 190

Query: 322 DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF 381
           DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF
Sbjct: 191 DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF 250

Query: 382 PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS 441
           PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS
Sbjct: 251 PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS 310

Query: 442 LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES 501
           LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES
Sbjct: 311 LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES 370

Query: 502 TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE 561
           TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE
Sbjct: 371 TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE 430

Query: 562 LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT 621
           LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT
Sbjct: 431 LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT 490

Query: 622 RPVLLSML 630
           RPVLLSML
Sbjct: 491 RPVLLSML 498

BLAST of IVF0001740 vs. ExPASy TrEMBL
Match: A0A6J1HF51 (uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC111462961 PE=4 SV=1)

HSP 1 Score: 959.9 bits (2480), Expect = 5.2e-276
Identity = 493/640 (77.03%), Postives = 541/640 (84.53%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           M YENFDP+FP+QPVVD YLPVWASLPAF SKPAFIW+EDGT + +NEGSFLTY QLHD+
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQ I+++L+RQ+RRRDTVV+LCS GLD VQLIYGCQRAGLVSVPISPPD   ENENCHHL
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 133

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK-----EPHK 180
            RALSQTKPRAAIAHQ+YI +VFRY+S SP D KLALLL+SVQWISME+L+     E  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEADQ 193

Query: 181 ESELNQHKHHSFSHHS-SSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARK 240
              +   K   F +HS SSY GC P++PYLIQYTSGAT I KPVV+TAGAAAHNVRAARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 253

Query: 241 AYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQ+HDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLD 360
           CTPVPSFTLPLVLKRV    +   G LDL SLRNLILINEP+YRS VEEFVDVFKAVGL+
Sbjct: 314 CTPVPSFTLPLVLKRV-KEETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRKLLPSGRLRDGMCKEIEVVVV 420
           PGCVSPSYGLAENCTFVSTAW GG G     FPAMPSYRKLLPS RL D    EIEVVVV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIK 480
           N ETGE+VEDGVEGEIWISSPSNA GYLGHPSLT +TFH KL NK+S  FVRTGDRGVIK
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIK 493

Query: 481 GSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALV 540
           G+DRFLFVIGRCSDVI    NNQ+IHPHYIES +Y N S+YLRGGC+AA+KIS  T+A+V
Sbjct: 494 GTDRFLFVIGRCSDVITLT-NNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISD-TIAVV 553

Query: 541 AEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEK 600
           AEMQR D+NDAE LRKICE IRKA LIEEGIELG+VVLVKRGNV KTTSGKVKRW  KEK
Sbjct: 554 AEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEK 613

Query: 601 LGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 630
           L GGG+ VLMAV+FGK C   KDLE       RP+LLS+L
Sbjct: 614 LAGGGMSVLMAVKFGKTCGVTKDLET-----NRPLLLSLL 645

BLAST of IVF0001740 vs. ExPASy TrEMBL
Match: A0A6J1K7R6 (uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429 PE=4 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 5.4e-273
Identity = 487/640 (76.09%), Postives = 539/640 (84.22%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           M YENFDP+FP+QPVVD YLPVWASLPAF SKPAFIW+EDGT + +NEGSFLTY QLHD+
Sbjct: 1   MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 60

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQ I+EQL+RQ+RRRDTV+VLCS GLD VQLIYGCQRAGLVSVPISPPD   ENENCHH 
Sbjct: 61  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENENCHHF 120

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK-----EPHK 180
            RALSQTKPRA IAH+ YI +VFRY+SSS  D KLALLL+SVQWISME+L+     E  +
Sbjct: 121 ARALSQTKPRAVIAHEPYIVSVFRYLSSSQIDGKLALLLQSVQWISMETLRTCTAAEADQ 180

Query: 181 ESELNQHKHHSFSHHS-SSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARK 240
              +   K   F +HS SSY GC P++PYLIQYTSGAT I KPVV+TAGAAAHNVRAARK
Sbjct: 181 VVSVISSKDRPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 240

Query: 241 AYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQ+HDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 241 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 300

Query: 301 CTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLD 360
           CTPVPSFTLPLVLKRV    +   G+LDL  LRNLILINEP+YRS VEEFVDVFKAVGL+
Sbjct: 301 CTPVPSFTLPLVLKRV-KEETPPGGDLDLCCLRNLILINEPVYRSGVEEFVDVFKAVGLN 360

Query: 361 PGCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRKLLPSGRLRDGMCKEIEVVVV 420
           PGCVSPSYGLAEN TFVSTAW GG G     FPAMPSYRKLLP  RL DG   EIEVVVV
Sbjct: 361 PGCVSPSYGLAENSTFVSTAWSGGEGGLRRCFPAMPSYRKLLPCARLTDGSSAEIEVVVV 420

Query: 421 NEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIK 480
           N ETGE+V+DGVEGEIWISSPSNA GYLGHP LT +TFH KL NK+S  FVRTGDRGVIK
Sbjct: 421 NGETGEMVDDGVEGEIWISSPSNASGYLGHPLLTRDTFHCKLSNKSSHKFVRTGDRGVIK 480

Query: 481 GSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALV 540
           G+DRFLFVIGRCSDVI    NNQ+IHPHYIES +Y NFSAYLRGGC+AA+K+S  T+A+V
Sbjct: 481 GADRFLFVIGRCSDVITLT-NNQEIHPHYIESIAYNNFSAYLRGGCLAAIKVSD-TIAVV 540

Query: 541 AEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEK 600
           AEMQR+D+NDAE LR+ICE IRKA LIEEGIELG+VVLVKRGNV KTTSGKVKRW+ KEK
Sbjct: 541 AEMQREDKNDAETLRQICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWLAKEK 600

Query: 601 LGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 630
           L GGG+ VLMAV+FG  C   KDL+     G RP+LLS+L
Sbjct: 601 LAGGGMSVLMAVKFGTICGVTKDLD-----GNRPLLLSLL 632

BLAST of IVF0001740 vs. ExPASy TrEMBL
Match: A0A1S3BAI6 (long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC103487973 PE=4 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 3.7e-266
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 167 MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 226
           MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA
Sbjct: 1   MESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAA 60

Query: 227 HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 286
           HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH
Sbjct: 61  HNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLH 120

Query: 287 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 346
           LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD
Sbjct: 121 LITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVD 180

Query: 347 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEV 406
           VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEV
Sbjct: 181 VFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEV 240

Query: 407 VVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRG 466
           VVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRG
Sbjct: 241 VVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRG 300

Query: 467 VIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTV 526
           VIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTV
Sbjct: 301 VIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTV 360

Query: 527 ALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVV 586
           ALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVV
Sbjct: 361 ALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVV 420

Query: 587 KEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 630
           KEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML
Sbjct: 421 KEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 463

BLAST of IVF0001740 vs. NCBI nr
Match: XP_011649625.1 (uncharacterized protein LOC105434645 [Cucumis sativus] >KGN62605.1 hypothetical protein Csa_021877 [Cucumis sativus])

HSP 1 Score: 1151 bits (2977), Expect = 0.0
Identity = 581/629 (92.37%), Postives = 604/629 (96.03%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           MLYENFDPLFPEQPVVDRYLPVWASLPAF SKPAFIW+EDGT +AINE SFLTYRQLHD+
Sbjct: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFQSKPAFIWSEDGTAKAINEDSFLTYRQLHDS 60

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL
Sbjct: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELN 180
            RALSQTKPRAAIAHQSYI TVFRYISSS AD+KLALLLK V+WISMESLK+PHKESELN
Sbjct: 121 ARALSQTKPRAAIAHQSYINTVFRYISSS-ADKKLALLLKGVRWISMESLKQPHKESELN 180

Query: 181 QHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNP 240
           QHKH S  +HSSSYSGCNPDDPYLIQYTSGATAISK VVITAGAAAHNVRAARKAYDLNP
Sbjct: 181 QHKHQSSFYHSSSYSGCNPDDPYLIQYTSGATAISKAVVITAGAAAHNVRAARKAYDLNP 240

Query: 241 NDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300
           ND+IVSWLPQ+HDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS
Sbjct: 241 NDLIVSWLPQYHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSP 360
           FTLPLVLKRVG YSSGRAGNLDLRSLRNLILINEPIYRSTVEEFV+VFK VGLDPGCVSP
Sbjct: 301 FTLPLVLKRVG-YSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVEVFKTVGLDPGCVSP 360

Query: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420
           SYGLAENCTFVSTAWCGGGGFPAMPSYR+LLPSGRLRDGMCKEIEVVVVNEETGEVVEDG
Sbjct: 361 SYGLAENCTFVSTAWCGGGGFPAMPSYRQLLPSGRLRDGMCKEIEVVVVNEETGEVVEDG 420

Query: 421 VEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGR 480
           VEGEIW+SSPSNALGYLGHPS+TEETFH KLKNK+SLNFVRTGDRGVIKGSDRFLFVIGR
Sbjct: 421 VEGEIWVSSPSNALGYLGHPSVTEETFHSKLKNKSSLNFVRTGDRGVIKGSDRFLFVIGR 480

Query: 481 CSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDA 540
           CSDVIK N+NNQQIHPHYIESTSY NFSAYLRGGCIAAVK+S GT+ALVAEMQRDDR+DA
Sbjct: 481 CSDVIKLNNNNQQIHPHYIESTSYNNFSAYLRGGCIAAVKVS-GTIALVAEMQRDDRHDA 540

Query: 541 ELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMA 600
           ELLRKICE+IRKAVLIEEGIELG+VVLVKRGNVSKTTSGKVKRWVVKEKL GGGL VLMA
Sbjct: 541 ELLRKICEDIRKAVLIEEGIELGLVVLVKRGNVSKTTSGKVKRWVVKEKLAGGGLGVLMA 600

Query: 601 VEFGKDCEGLKDLERKGEFGTRPVLLSML 629
            EFGK+C    DL+RKGEF TRPVL+SML
Sbjct: 601 FEFGKNCV---DLKRKGEFETRPVLISML 623

BLAST of IVF0001740 vs. NCBI nr
Match: XP_038884878.1 (long-chain-fatty-acid--AMP ligase FadD26-like [Benincasa hispida])

HSP 1 Score: 1025 bits (2649), Expect = 0.0
Identity = 513/631 (81.30%), Postives = 566/631 (89.70%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           MLYENFDPLFP+QPVVDRYLPVWASLPAF SKPAFIW+EDGT  ++N+GSFLTY+QLHD+
Sbjct: 1   MLYENFDPLFPDQPVVDRYLPVWASLPAFRSKPAFIWSEDGTAGSLNDGSFLTYQQLHDS 60

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQ I++QLLRQLRRRDTV VLCSAGL+ VQLIYGCQRAGLVSVPISPPD F ENENCHHL
Sbjct: 61  VQFISDQLLRQLRRRDTVAVLCSAGLELVQLIYGCQRAGLVSVPISPPDPFLENENCHHL 120

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELN 180
            RALSQTKPRAAIAHQSYI T+FRY+SSSP DEKLALLL+SVQWISM+SLK+P K++E+N
Sbjct: 121 ARALSQTKPRAAIAHQSYIYTIFRYLSSSPTDEKLALLLQSVQWISMDSLKQPDKKAEVN 180

Query: 181 QHKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNP 240
           Q KH    +HSSSY GCNP +PYLIQYTSGATAI KPVV+TAGAAAHNVR ARKAY+LNP
Sbjct: 181 QDKHQPILYHSSSYYGCNPKEPYLIQYTSGATAIPKPVVVTAGAAAHNVRVARKAYNLNP 240

Query: 241 NDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPS 300
           NDVIVSWLPQ+HDCGLMFLLL+VITGATCVLTSP+SFVT PI WLHLITAFKATCTPVPS
Sbjct: 241 NDVIVSWLPQYHDCGLMFLLLSVITGATCVLTSPMSFVTNPIRWLHLITAFKATCTPVPS 300

Query: 301 FTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSP 360
           FTLPLVLKRV + +    G LDLRSLRNLILINEPIYR+ VEEFVD FKAVGLDPGCVSP
Sbjct: 301 FTLPLVLKRVKDETPSSKG-LDLRSLRNLILINEPIYRAAVEEFVDEFKAVGLDPGCVSP 360

Query: 361 SYGLAENCTFVSTAWCGGGG--FPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVE 420
           SYGLAENCTFVSTAW GG    FPAMP+YRKLLPSGRLRDG+C EI+VVVVN ETGEVVE
Sbjct: 361 SYGLAENCTFVSTAWSGGRRWWFPAMPNYRKLLPSGRLRDGLCTEIKVVVVNGETGEVVE 420

Query: 421 DGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVI 480
           DGVEGEIW+SSPSNA GYLGHPSLT++TFH K+ NK+S NF+RTGDRGVI+G+DRFLFVI
Sbjct: 421 DGVEGEIWVSSPSNASGYLGHPSLTKKTFHSKINNKSSPNFIRTGDRGVIRGADRFLFVI 480

Query: 481 GRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRN 540
           GRCSD+IKFN+  Q+IHPHYIEST+Y N SAYLRGGCIAAVKIS  T+A+VAEMQRDD+N
Sbjct: 481 GRCSDLIKFNNTQQEIHPHYIESTAYNNSSAYLRGGCIAAVKISE-TIAVVAEMQRDDKN 540

Query: 541 DAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVL 600
           DAELLRKICEEIRKAV IEEG ELG+VVLVKRGN+ KTTSGKVKRW VKEKL GGGL VL
Sbjct: 541 DAELLRKICEEIRKAVSIEEGTELGLVVLVKRGNIPKTTSGKVKRWAVKEKLAGGGLGVL 600

Query: 601 MAVEFGKDCEGLKDLERKGEFGTRPVLLSML 629
           MA+ F K+CE L   +R+ E  TRP LLS+L
Sbjct: 601 MAIGFEKNCEVL---QREEELRTRPALLSLL 626

BLAST of IVF0001740 vs. NCBI nr
Match: KAA0065230.1 (long-chain-fatty-acid--AMP ligase FadD26-like [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2509), Expect = 0.0
Identity = 486/488 (99.59%), Postives = 488/488 (100.00%), Query Frame = 0

Query: 142 VFRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD 201
           ++RYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD
Sbjct: 11  IYRYISSSPADEKLALLLKSVQWISMESLKEPHKESELNQHKHHSFSHHSSSYSGCNPDD 70

Query: 202 PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL 261
           PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL
Sbjct: 71  PYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLMFLLL 130

Query: 262 TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL 321
           TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL
Sbjct: 131 TVITGATCVLTSPISFVTQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNL 190

Query: 322 DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF 381
           DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF
Sbjct: 191 DLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGF 250

Query: 382 PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS 441
           PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS
Sbjct: 251 PAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPS 310

Query: 442 LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES 501
           LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES
Sbjct: 311 LTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIES 370

Query: 502 TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE 561
           TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE
Sbjct: 371 TSYKNFSAYLRGGCIAAVKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIE 430

Query: 562 LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT 621
           LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT
Sbjct: 431 LGVVVLVKRGNVSKTTSGKVKRWVVKEKLGGGGLEVLMAVEFGKDCEGLKDLERKGEFGT 490

Query: 622 RPVLLSML 629
           RPVLLSML
Sbjct: 491 RPVLLSML 498

BLAST of IVF0001740 vs. NCBI nr
Match: XP_023546350.1 (uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 961 bits (2484), Expect = 0.0
Identity = 493/640 (77.03%), Postives = 541/640 (84.53%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           M YENFDP+FP+QPVVD YLPVWASLPAF SKPAFIW+EDGT + +NEGSFLTY QLHD+
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQ I+EQL+RQ+RRRDTV+VLCS GLD VQLIYGCQRAGLVSVPISPPD   EN+NCHHL
Sbjct: 74  VQFISEQLIRQVRRRDTVIVLCSPGLDLVQLIYGCQRAGLVSVPISPPDPCFENKNCHHL 133

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK-----EPHK 180
            RALSQTKPRAAIAHQ+YI +VFRY+S SP D KLALLL+ VQWISME+L+     E  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLRGVQWISMETLRTCTAAEADQ 193

Query: 181 ESELNQHKHHSFSHHS-SSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARK 240
              +   K   F +HS SSY GC P++PYLIQYTSGAT I KPVV+TAGAAAHNVR ARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRVARK 253

Query: 241 AYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQ+HDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLD 360
           CTPVPSFTLPLVLKRV    +   G LDL SLRNLILINEP+YRS VEEFVDVFK VGL+
Sbjct: 314 CTPVPSFTLPLVLKRVKE-ETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKTVGLN 373

Query: 361 PGCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRKLLPSGRLRDGMCKEIEVVVV 420
           PGCVSPSYGLAENCTFVSTAW GG G     FPAMPSYRKLLP  +L D    EIEVVVV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPCAKLTDESSAEIEVVVV 433

Query: 421 NEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIK 480
           N ETGE+V+DGVEGEIWISSPSNA GYLGHPSLT +TFH KL NK+S  FVRTGDRGVIK
Sbjct: 434 NGETGEIVDDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIK 493

Query: 481 GSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALV 540
           G+DRFLFVIGRCSDVIK N NNQ+IHPHYIES +Y NFSAYLRGGC+AA+KIS  T+A+V
Sbjct: 494 GTDRFLFVIGRCSDVIKLN-NNQEIHPHYIESIAYNNFSAYLRGGCLAAIKISD-TIAVV 553

Query: 541 AEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEK 600
           AEMQRDD+NDAE LRKICE IRKA LIEEGIELG+VVLVKRGNV KTTSGKVKRW  KEK
Sbjct: 554 AEMQRDDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEK 613

Query: 601 LGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 629
           L GGG+ VLMAV+FG  C   KDLE     G RP+LLS+L
Sbjct: 614 LAGGGMSVLMAVKFGTTCGVTKDLE-----GNRPLLLSLL 645

BLAST of IVF0001740 vs. NCBI nr
Match: XP_022962563.1 (uncharacterized protein LOC111462961 [Cucurbita moschata])

HSP 1 Score: 957 bits (2474), Expect = 0.0
Identity = 493/640 (77.03%), Postives = 541/640 (84.53%), Query Frame = 0

Query: 1   MLYENFDPLFPEQPVVDRYLPVWASLPAFGSKPAFIWTEDGTVEAINEGSFLTYRQLHDT 60
           M YENFDP+FP+QPVVD YLPVWASLPAF SKPAFIW+EDGT + +NEGSFLTY QLHD+
Sbjct: 14  MSYENFDPIFPDQPVVDLYLPVWASLPAFRSKPAFIWSEDGTADVLNEGSFLTYGQLHDS 73

Query: 61  VQLITEQLLRQLRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPDVFSENENCHHL 120
           VQ I+++L+RQ+RRRDTVV+LCS GLD VQLIYGCQRAGLVSVPISPPD   ENENCHHL
Sbjct: 74  VQFISDELIRQVRRRDTVVILCSPGLDLVQLIYGCQRAGLVSVPISPPDPRFENENCHHL 133

Query: 121 GRALSQTKPRAAIAHQSYITTVFRYISSSPADEKLALLLKSVQWISMESLK-----EPHK 180
            RALSQTKPRAAIAHQ+YI +VFRY+S SP D KLALLL+SVQWISME+L+     E  +
Sbjct: 134 ARALSQTKPRAAIAHQAYIASVFRYLSLSPTDGKLALLLQSVQWISMETLRTCTAAEADQ 193

Query: 181 ESELNQHKHHSFSHHS-SSYSGCNPDDPYLIQYTSGATAISKPVVITAGAAAHNVRAARK 240
              +   K   F +HS SSY GC P++PYLIQYTSGAT I KPVV+TAGAAAHNVRAARK
Sbjct: 194 VVSVISSKDQPFFYHSNSSYYGCKPEEPYLIQYTSGATGIPKPVVVTAGAAAHNVRAARK 253

Query: 241 AYDLNPNDVIVSWLPQFHDCGLMFLLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT 300
           AYDLNPNDVIVSWLPQ+HDCGLMFLLLTV++GATCVLTSPISFVT+PITWLHLIT FKAT
Sbjct: 254 AYDLNPNDVIVSWLPQYHDCGLMFLLLTVVSGATCVLTSPISFVTRPITWLHLITTFKAT 313

Query: 301 CTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLD 360
           CTPVPSFTLPLVLKRV    +   G LDL SLRNLILINEP+YRS VEEFVDVFKAVGL+
Sbjct: 314 CTPVPSFTLPLVLKRVKE-ETPPCGGLDLCSLRNLILINEPVYRSGVEEFVDVFKAVGLN 373

Query: 361 PGCVSPSYGLAENCTFVSTAWCGGGG-----FPAMPSYRKLLPSGRLRDGMCKEIEVVVV 420
           PGCVSPSYGLAENCTFVSTAW GG G     FPAMPSYRKLLPS RL D    EIEVVVV
Sbjct: 374 PGCVSPSYGLAENCTFVSTAWSGGEGGRRRWFPAMPSYRKLLPSARLMDESSSEIEVVVV 433

Query: 421 NEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLNFVRTGDRGVIK 480
           N ETGE+VEDGVEGEIWISSPSNA GYLGHPSLT +TFH KL NK+S  FVRTGDRGVIK
Sbjct: 434 NGETGEIVEDGVEGEIWISSPSNASGYLGHPSLTRDTFHCKLSNKSSHKFVRTGDRGVIK 493

Query: 481 GSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAAVKISSGTVALV 540
           G+DRFLFVIGRCSDVI    NNQ+IHPHYIES +Y N S+YLRGGC+AA+KIS  T+A+V
Sbjct: 494 GTDRFLFVIGRCSDVITLT-NNQEIHPHYIESIAYNNCSSYLRGGCLAAIKISD-TIAVV 553

Query: 541 AEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTSGKVKRWVVKEK 600
           AEMQR D+NDAE LRKICE IRKA LIEEGIELG+VVLVKRGNV KTTSGKVKRW  KEK
Sbjct: 554 AEMQRQDKNDAESLRKICEGIRKAALIEEGIELGLVVLVKRGNVPKTTSGKVKRWAAKEK 613

Query: 601 LGGGGLEVLMAVEFGKDCEGLKDLERKGEFGTRPVLLSML 629
           L GGG+ VLMAV+FGK C   KDLE       RP+LLS+L
Sbjct: 614 LAGGGMSVLMAVKFGKTCGVTKDLET-----NRPLLLSLL 645

BLAST of IVF0001740 vs. TAIR 10
Match: AT5G38120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 67.8 bits (164), Expect = 3.6e-11
Identity = 123/545 (22.57%), Postives = 213/545 (39.08%), Query Frame = 0

Query: 52  LTYRQLHDTVQLITEQLLRQ--LRRRDTVVVLCSAGLDFVQLIYGCQRAGLVSVPISPPD 111
           +++  L   V  + + LL    +RR D V+VL    +    +       G V    +P +
Sbjct: 65  ISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAVLTTANPLN 124

Query: 112 VFSENENCHHLGRALSQTKPRAAIA--------HQSYITTVFRYISSSPADEKLALLLKS 171
             SE      + R ++ + P+ A            S I+ V   +  +    ++   LK 
Sbjct: 125 TASE------ILRQIADSNPKLAFTTPELAPKIASSGISIVLERVEDT---LRVPRGLKV 184

Query: 172 VQWISMESLKEPHKESELNQ-HKHHSFSHHSSSYSGCNPDDPYLIQYTSGATAISKPVVI 231
           V  ++    KEP  ++  NQ HK                DD  ++ Y+SG T  SK V  
Sbjct: 185 VGNLTEMMKKEPSGQAVRNQVHK----------------DDTAMLLYSSGTTGRSKGVNS 244

Query: 232 TAG-AAAHNVRAARKAYDLNPNDVIVSWLPQFHDCGLM-FLLLTVITGATCVLTSPISFV 291
           + G   AH  R   + ++  P    +  +P FH  GL+ F+L T+  G T V+       
Sbjct: 245 SHGNLIAHVARYIAEPFE-QPQQTFICTVPLFHTFGLLNFVLATLALGTTVVILPRFDLG 304

Query: 292 TQPITWLHLITAFKATCTPVPSFTLPLVLKRVGNYSSGRAGNLDLRSLRNLILINEPIYR 351
                 +  +  ++AT        +P VL  + N +       D+  LR +     P+ +
Sbjct: 305 EM----MAAVEKYRATTL----ILVPPVLVTMINKADQIMKKYDVSFLRTVRCGGAPLSK 364

Query: 352 STVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCGGGGFPAMPSYRKLLPSGRLRD 411
              + F+  +  V +  G     Y L E+          G    ++   R+    G L  
Sbjct: 365 EVTQGFMKKYPTVDVYQG-----YALTESNG-------AGASIESVEESRRYGAVGLLSC 424

Query: 412 GMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYLGHPSLTEETFHRKLKNKASLN 471
           G    +E  +V+  TG+V+     GE+W+  PS A GY          F  + +   S  
Sbjct: 425 G----VEARIVDPNTGQVMGLNQTGELWLKGPSIAKGY----------FRNEEEIITSEG 484

Query: 472 FVRTGDRGVIKGSDRFLFVIGRCSDVIKFNDNNQQIHPHYIESTSYKNFSAYLRGGCIAA 531
           +++TGD   I  +D FLF++ R  ++IK+     Q+ P  +E+    N    L    I  
Sbjct: 485 WLKTGDLCYI-DNDGFLFIVDRLKELIKY--KGYQVPPAELEAL-LLNHPDILDAAVIPF 539

Query: 532 VKISSGTVALVAEMQRDDRNDAELLRKICEEIRKAVLIEEGIELGVVVLVKRGNVSKTTS 584
               +G   +    ++ + N  E  +K+ + I K V   + I     +     ++ KT S
Sbjct: 545 PDKEAGQFPMAYVARKPESNLCE--KKVIDFISKQVAPYKKIRKVAFI----DSIPKTPS 539

BLAST of IVF0001740 vs. TAIR 10
Match: AT4G05160.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 66.2 bits (160), Expect = 1.0e-10
Identity = 79/290 (27.24%), Postives = 117/290 (40.34%), Query Frame = 0

Query: 201 DPYLIQYTSGATAISKPVVITAGAAAHNVRAARKAYDL--NPNDVIVSWLPQFHDCGLMF 260
           D   + Y+SG T  SK V +T G             DL    + V + +LP FH  GL  
Sbjct: 191 DTAALLYSSGTTGTSKGVELTHGNFIAASLMVTMDQDLMGEYHGVFLCFLPMFHVFGLAV 250

Query: 261 LLLTVITGATCVLTSPISFVTQPITWLHLITAFKAT-CTPVPSFTLPLVLKRVGNYSSGR 320
           +  + +      L S   F  + +  L  I  F+ T    VP   L L  + +       
Sbjct: 251 ITYSQLQRGN-ALVSMARFELELV--LKNIEKFRVTHLWVVPPVFLALSKQSI------- 310

Query: 321 AGNLDLRSLRNLILINEPIYRSTVEEFVDVFKAVGLDPGCVSPSYGLAENCTFVSTAWCG 380
               DL SL+ +     P+ +  +EE       V L  G     YG+ E C  VS     
Sbjct: 311 VKKFDLSSLKYIGSGAAPLGKDLMEECGRNIPNVLLMQG-----YGMTETCGIVSVE--- 370

Query: 381 GGGFPAMPSYRKLLPSGRLRDGMCKEIEVVVVNEETGEVVEDGVEGEIWISSPSNALGYL 440
                     R    +G L  G    +E  +V+ ETG+      +GEIW+  P+   GYL
Sbjct: 371 ----DPRLGKRNSGSAGMLAPG----VEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGYL 430

Query: 441 GHPSLTEETFHRKLKNKASLNFVRTGDRGVIKGSDRFLFVIGRCSDVIKF 488
            +P  T+ET  +K       ++V TGD G     D  L+V+ R  ++IK+
Sbjct: 431 NNPQATKETIDKK-------SWVHTGDLGYF-NEDGNLYVVDRIKELIKY 446

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P9WQ441.0e-4227.26Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain CDC... [more]
P9WQ451.0e-4227.26Putative fatty-acid--CoA ligase fadD25 OS=Mycobacterium tuberculosis (strain ATC... [more]
B2HIL41.9e-4127.674-hydroxyphenylalkanoate adenylyltransferase OS=Mycobacterium marinum (strain AT... [more]
P9WQ581.6e-4027.38Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain C... [more]
P9WQ591.6e-4027.38Long-chain-fatty-acid--AMP ligase FadD28 OS=Mycobacterium tuberculosis (strain A... [more]
Match NameE-valueIdentityDescription
A0A0A0LL230.0e+0092.37AMP-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G361690... [more]
A0A5A7VDG21.0e-27999.59Long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A6J1HF515.2e-27677.03uncharacterized protein LOC111462961 OS=Cucurbita moschata OX=3662 GN=LOC1114629... [more]
A0A6J1K7R65.4e-27376.09uncharacterized protein LOC111492429 OS=Cucurbita maxima OX=3661 GN=LOC111492429... [more]
A0A1S3BAI63.7e-266100.00long-chain-fatty-acid--AMP ligase FadD26-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
XP_011649625.10.092.37uncharacterized protein LOC105434645 [Cucumis sativus] >KGN62605.1 hypothetical ... [more]
XP_038884878.10.081.30long-chain-fatty-acid--AMP ligase FadD26-like [Benincasa hispida][more]
KAA0065230.10.099.59long-chain-fatty-acid--AMP ligase FadD26-like [Cucumis melo var. makuwa][more]
XP_023546350.10.077.03uncharacterized protein LOC111805474 [Cucurbita pepo subsp. pepo][more]
XP_022962563.10.077.03uncharacterized protein LOC111462961 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G38120.13.6e-1122.57AMP-dependent synthetase and ligase family protein [more]
AT4G05160.11.0e-1027.24AMP-dependent synthetase and ligase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 32..487
e-value: 3.5E-71
score: 240.0
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 9..481
e-value: 8.3E-94
score: 317.0
NoneNo IPR availableGENE3D3.30.300.30coord: 483..604
e-value: 1.5E-16
score: 62.9
NoneNo IPR availablePANTHERPTHR22754DISCO-INTERACTING PROTEIN 2 DIP2 -RELATEDcoord: 1..611
NoneNo IPR availablePANTHERPTHR22754:SF3510A19I.5coord: 1..611
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 26..592

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0001740.1IVF0001740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
molecular_function GO:0016874 ligase activity