IVF0001602 (gene) Melon (IVF77) v1

Overview
NameIVF0001602
Typegene
OrganismCucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Descriptionsubtilisin-like protease SBT5.3
Locationchr06: 23663740 .. 23669081 (-)
RNA-Seq ExpressionIVF0001602
SyntenyIVF0001602
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTATTATATATACTCTATTTACATTAGTGACTTCTTTTTTATTATTATTCTTATGTTTTCTATTGATCTTATCGATTTTGAACCTAGGAAAATGTAAAGATATTTTTAAAAAATTAATACATTAATAAATATCTAGATATCAAGTTTTCATTTCGTAAGTCAATGATTCAAATCTCTGGTTTCTAGAATTGTGACTTAAAAAAACCAACTTTCTAGGATGAAACTACCTATACAAATTTCCATTTTTATAACCTTGACAATAATAATTATATATATCCTTATCATTCTCAACAAAAACTTTCTAATTTTATTGAACCAAAAGATTTCTCATCCAGTGATTTTTTTCCCTTTTGATTTTCTGTATGTTCTAAAGATAAACTCATCGTTGTTCAAACGGAACAACAAAGTTCAGGTTTTAAATCTTAATTAAGCAAATACTTTTCCTCTATTGTATAAATAAAACCCCTTTGAAACGAAAAAAGAAATAATCTCAAGCAAAACCAATGGAGGCTTCCAATCTTTCTCCATTGCTTTTGTTCTTCTTCTACTTCTCTCTATTGCAAACTTCCACCATTGCCACCAAAAGGTTCGATAAGTTCATGCGTATGTTGAGAGCTGCATTCATCTTCATCCAATTGTCCAACTACAGTTTTTGACATGATTGGATGCAGCTTGGGATAATGCATCTAACTATTGTTCATATTTGTTATTTGTTCAAATCTCTATGTTCTTGTGTTGATTGTTTAATTCTTTTTTTGTGGTGCGACGCAGTCTTACATCGTTTACTTGGGTTCGGAACATTCCTCATCTTTGGATCCTTCCTCTTTATCAGAGCATTCCCAACAAGTTACTGCATTACATTATGATTTGTTGGGATCTTCGTTGGGAAGGTAAGAGCTGACTTACCATTCGAAATGACAGTACTCATGAGTTGATTAATTAACTTAATTTGATTCCATCGTTAAAGTTCAAAATTCTATCTATAATTTGTGTTTGGCGAGTGACTGTTTTATCATTACAACTTTGTCACAACCACATAGCAAAGATAACAAATCCAATCGTCAACCTTTAATAAATGTTCATCATTACTTCATCAACCAAATATATTAAATACCTGCAGAAAAACAAAACATCCTCCCTATTTCCTAACTATGATGCATCTTATATATTGCACTAAATATTTTTAGATATCTAATAGATATCTCAATGCATTTGAAGAGTTTATTAAATCACTTCAATTATTGTTTCTCTCATAAGCATGCATGAACGCAATTTTTATTTTCCTGTTTTATGACTAAACTTTCAATTGTGTAAACAGTAAAACGATGGCTGAGGAAGCGATTTTCTACTCATATACAAGAAGTTTCAATGGTTTCGCTGCCATACTTGATGACAAAGAAGCTGAAAATCTAGCAAGTAAAAATAACTTTTACATTATCTCTTAATTACTTGATATTTATATATGAGTTATATATGAATATATATTTGCTCACATAGATTAGTTTTTGTTTATTGTTTCTTTATATTATTTAATTACTATAAGATTCATTTGAGATATTAATAATAATCTTTATTTTAATGATGAGATTGATGAGGAGATTGATGTTTAATAAAACATTGGCATACAGGAAACCCTAAGGTGATATCAGTGTTTGAAAATAAGGCAAGAAAATTGCACACAACACGTTCTTGGAACTTTCTTGGAGTGGAAAATGATATCGGAATTCCTTCAAATTCCATTTGGAATGCTGCAAAGTTTGGTGAAGATGTAATCATAGCCAACATTGACACAGGTAATTTTATTTTTTCTCTTTACTATTTACTCTTTCCAAAATTAATAATAATTTCTTTTCTTCTTTTTCTCTTATCTACGGAAAATAATTGCTTTAAATGACAAAACTTAACGTGTATATGTATATATATATGAACTCGAGATTTGATTTAATCTAATTTTGTATGTTGAGCTCAAAACCAAAACCAAAATTACACAGTCCAACCTTGACATATGCATTACTTAACCGGTTAAGGCATTTTGTTGAATTAAATACAAACGAAAGGTGATGATTGACAATGCGCGAATGAGGTTTGAGAATCTTTTTATGTATTATTGATCATATGATGATTGGGAAATTTGGGATTTGTTTATTGTGAAGGTGTTTGGCCAGAATCAAAGAGCTTTAGTGATGAAGGGTATGGACCTGTGCCATCAAAATGGAGAGGCATTTGTCAAACTGACTCCAACTTCCTTTGCAATAGGTTTCATATCTTCAACTTTTTCATTTTTATTTTCTCTCTAAATTGATGACCTATCTAGAGATTAATTTCTTCACTGAATAGTCAAATTTAATTGCTATTCATAACATTTTTTCTAAAATTTAACCATATTAAAATGGAATATTAAAATGGAAAATGATTACGATTGAAGGAATAAATTCTCATTGATTTAAAAAAATTTATTAGAAAAAGAATTATTCAAACTAATGTGTTTTTTTTTTTAATTATAAAACATTAAAAATTATAAATTATTTAGGCAAAGAAAATTTGAAAATTGAAATATTGATGGAAAATATCCAGGAAGCTTATTGGAGGAAGATATTTCCACAAAGGATATGAAGCAGCCGGAGGCAAACTCAACGCCACTTTACTCACCGTACGCGACCACGACGGCCATGGAACCCACACTCTCTCAACCGCTGCCGGAAACTTTGTCACCGGAGCCAACGTTTTTGGCCATGGTAATGGCACGGCCAAAGGTGGTGCTCCGAAGGCTCGCGCCGTTGCCTACAAGGTCTGCTGGCCTCCGCTCTTTGACTCACAATGCTTCGACGCCGATATTCTTGCCGCCTTCGAAGCCGCTATTGCCGATGGAGTCGACGTGCTATCAACTTCCCTTGGTGGAGCAGCCGACGAATATTTCAACGATCCACTTGCTATCGCCGCCTTCCTCGCCGTTCAACGAGGCATTCTCGTTGTCTTCTCTGGTGGCAATTCCGGTCCTTTCCCAATGACCGTCACCAATGTTTCCCCTTGGGTATTCACCGTCGCGGCCAACACCATAGACAGAGATTTCGTCAGTTATGTGGGGCTTGGAAACAAGAAACACATCAAGGTACACGTATATACTCATCTTTTAGGGTTAGGTGCATCTCGGTTTGCACCCAACTTTATTTGTGCACCCGAATTATATAATCAATATTCAATAGGTTCAACTCTAGAAACATCGAAGTATTCTAGAAAAAATGTTTACCGGTTTTTACTCCATAACATTTCACCATTTTTTTTCCATGGTAGGGTTTAAGCCTTTCTTCAGTTGCTTCATTACCAAAGAAGTTCTATCCATTGATCAATTCAGTGGATGCAAAATTCAGCAATGTCACCGAATTCCATGCGTAAGTTTTTGAGATTTTTCATAGCTTCCCTATATGTTTCTTTGGATGAAACATATGAGTTTATAATGTGTCTATTTGCAGTCAATTTTGCGGCAAGGGGACGCTTGATCCAATGAAAGTAAAGGGAAAGATAGTGATTTGTCAAGTAGGGGAGACCGAAGGAGTAGACAAGGGCTACCAAGCTTCTCTAGCTGGTGCCGCAGGAGTAATTGTAGCTAACGATATCGAAAAAGGAGACGAAATCTATCCCGAATTACACTTCATTCCCGCTTCGGATATTACGAATACCGACGCACAAATAGTCCAAAAGTATCTCAAATCCACCAAAACACCAATAGCTCATTTAACCAGTGTCAAAACATTGTTGAGTGTCAAACCAGCTCCAATCATTGCTACCTTCTCATCTAGAGGCCCCAACCCAATTGATAGTACCATTCTCAAGGTTTGCTTTTCTTTCCCAATTCTTGTTTTTCACTTCTTGTTTTGGAACTTTTATTTATTACTCTCTGTTGTTGTAGCCTGATGTCACAGCCCCTGGTGTGAATATACTGGCTTCTTATCCCACTGGAATTGCTCCGACGTTCTCCGCTGCTGACCGGCGGCGTATCCCATTTAATGTTATCTCTGGTACTTCCATGTCTTGTCCTCACGTTGCCGGTATCGCCGGTCTTGTTAAGTCCATTCATCCTAATTGGAGCCCTGCTGCTATCAAATCTGCTATTATGACTACTGGTATATGCTTCTTGCTGCCTCCTTGTGCTTTCCTAAGTTTGATCATTTTCAACATTTCTTCTTGATTATACCAAGATTGAAGTGTTGATAAGATTAAATTTAGATCAGCCTCTAGACTTCGAAATATTATAATTTTATTATTGATATTTGAGTTTTGTTTAGGTTGGTTTGCAGTAATTTTAAACCCTAATTTCTCATTAATTAAATTCTGAATTTTTGTGTTTAAGGTTAATATATATTGACCAAATTAAAAGATTTTAAAAAATTCTCATTAACCAAATTTTACTTAATTTTCATGATGACTATTAAAATTGAATTTAAAATTTTCCTTTATAATTATTTTTAATTAATTAATAGATATTAACATTAATGATAATAAATGAAAAATCAAAATTAATTAAAAGTTTAAATCTCGAAAATCAAATTGAAATAAAACTCAAATTTCTTAGTGCTAATTCTAATTAACGTCATGAGAAACTACACTAAAAACTGGACTAAATGTATAATATTTTGAAACATATAAACGAAATGAAAATTAGACCCGTCCACTAGTGAGTGGAAGTGCATAAAGTATTAGATATAATTAAAGTTTAAGTTCTTGAGTCAATTGACGATTTTACATATATCTTTATAGATTAATGACCTATTCTTTTGCAGCCAAAACAAGAGGCAACAACAACCAAACAATCCTCGACTCAACGAAACTCAAGGCCACACCTTACGCTTATGGTGCAGGACAAGTATATCCAAACGATGCAGCGGACCCCGGCCTCGTTTATGATATCACTATTAATGACTACTTAAACTTCTTGTGCGCTCGAGGCTACGATGCAATGAAAATTAAAAAATTCTATGCTAAGCCATTTAACTGTGTTAGATCGTTCAAAGTCACGGATCTCAACTACCCGTCGATCTCGGTCGGGGAGCTGAAAATTGGTGCTCCTTTGACAATCAATAGAAGAGTTAAGAACGTGGGAAGTCCAGGGACGTATGTCGCACGGGTCAAAGCGTCACCCGGTGTTGCGGTTACGATCGAGCCGAGCACATTGGTGTTTAGAAGTGTGGGTGAAGAGAAGGGGTTTAAGGTTGTATTGCAAAACACAGGAAAAGTGAAAAGTGGAGATGTGTTTGGAACATTGATTTGGTCTGATGGGAAGCACTTTGTTAGAAGTCCCATTGCTGTGCATTTGGGACCCGGCATTGGAAATTAATTTGTG

mRNA sequence

ATGTCTATTATATATACTCTATTTACATTAGTGACTTCTTTTTTATTATTATTCTTATGTTTTCTATTGATCTTATCGATTTTGAACCTAGGAAAATCGATTTTCTACTCATATACAAGAAGTTTCAATGGTTTCGCTGCCATACTTGATGACAAAGAAGCTGAAAATCTAGCAAGAAACCCTAAGGTGATATCAGTGTTTGAAAATAAGGCAAGAAAATTGCACACAACACGTTCTTGGAACTTTCTTGGAGTGGAAAATGATATCGGAATTCCTTCAAATTCCATTTGGAATGCTGCAAAGTTTGGTGAAGATGTAATCATAGCCAACATTGACACAGGTGTTTGGCCAGAATCAAAGAGCTTTAGTGATGAAGGGTATGGACCTGTGCCATCAAAATGGAGAGGCATTTGTCAAACTGACTCCAACTTCCTTTGCAATAGGAAGCTTATTGGAGGAAGATATTTCCACAAAGGATATGAAGCAGCCGGAGGCAAACTCAACGCCACTTTACTCACCGTACGCGACCACGACGGCCATGGAACCCACACTCTCTCAACCGCTGCCGGAAACTTTGTCACCGGAGCCAACGTTTTTGGCCATGGTAATGGCACGGCCAAAGGTGGTGCTCCGAAGGCTCGCGCCGTTGCCTACAAGGTCTGCTGGCCTCCGCTCTTTGACTCACAATGCTTCGACGCCGATATTCTTGCCGCCTTCGAAGCCGCTATTGCCGATGGAGTCGACGTGCTATCAACTTCCCTTGGTGGAGCAGCCGACGAATATTTCAACGATCCACTTGCTATCGCCGCCTTCCTCGCCGTTCAACGAGGCATTCTCGTTGTCTTCTCTGGTGGCAATTCCGGTCCTTTCCCAATGACCGTCACCAATGTTTCCCCTTGGGTATTCACCGTCGCGGCCAACACCATAGACAGAGATTTCGTCAGTTATGTGGGGCTTGGAAACAAGAAACACATCAAGGGTTTAAGCCTTTCTTCAGTTGCTTCATTACCAAAGAAGTTCTATCCATTGATCAATTCAGTGGATGCAAAATTCAGCAATGTCACCGAATTCCATGCTCAATTTTGCGGCAAGGGGACGCTTGATCCAATGAAAGTAAAGGGAAAGATAGTGATTTGTCAAGTAGGGGAGACCGAAGGAGTAGACAAGGGCTACCAAGCTTCTCTAGCTGGTGCCGCAGGAGTAATTGTAGCTAACGATATCGAAAAAGGAGACGAAATCTATCCCGAATTACACTTCATTCCCGCTTCGGATATTACGAATACCGACGCACAAATAGTCCAAAAGTATCTCAAATCCACCAAAACACCAATAGCTCATTTAACCAGTGTCAAAACATTGTTGAGTGTCAAACCAGCTCCAATCATTGCTACCTTCTCATCTAGAGGCCCCAACCCAATTGATAGTACCATTCTCAAGCCTGATGTCACAGCCCCTGGTGTGAATATACTGGCTTCTTATCCCACTGGAATTGCTCCGACGTTCTCCGCTGCTGACCGGCGGCGTATCCCATTTAATGTTATCTCTGGTACTTCCATGTCTTGTCCTCACGTTGCCGGTATCGCCGGTCTTGTTAAGTCCATTCATCCTAATTGGAGCCCTGCTGCTATCAAATCTGCTATTATGACTACTGCCAAAACAAGAGGCAACAACAACCAAACAATCCTCGACTCAACGAAACTCAAGGCCACACCTTACGCTTATGGTGCAGGACAAGTATATCCAAACGATGCAGCGGACCCCGGCCTCGTTTATGATATCACTATTAATGACTACTTAAACTTCTTGTGCGCTCGAGGCTACGATGCAATGAAAATTAAAAAATTCTATGCTAAGCCATTTAACTGTGTTAGATCGTTCAAAGTCACGGATCTCAACTACCCGTCGATCTCGGTCGGGGAGCTGAAAATTGGTGCTCCTTTGACAATCAATAGAAGAGTTAAGAACGTGGGAAGTCCAGGGACGTATGTCGCACGGGTCAAAGCGTCACCCGGTGTTGCGGTTACGATCGAGCCGAGCACATTGGTGTTTAGAAGTGTGGGTGAAGAGAAGGGGTTTAAGGTTGTATTGCAAAACACAGGAAAAGTGAAAAGTGGAGATGTGTTTGGAACATTGATTTGGTCTGATGGGAAGCACTTTGTTAGAAGTCCCATTGCTGTGCATTTGGGACCCGGCATTGGAAATTAATTTGTG

Coding sequence (CDS)

ATGTCTATTATATATACTCTATTTACATTAGTGACTTCTTTTTTATTATTATTCTTATGTTTTCTATTGATCTTATCGATTTTGAACCTAGGAAAATCGATTTTCTACTCATATACAAGAAGTTTCAATGGTTTCGCTGCCATACTTGATGACAAAGAAGCTGAAAATCTAGCAAGAAACCCTAAGGTGATATCAGTGTTTGAAAATAAGGCAAGAAAATTGCACACAACACGTTCTTGGAACTTTCTTGGAGTGGAAAATGATATCGGAATTCCTTCAAATTCCATTTGGAATGCTGCAAAGTTTGGTGAAGATGTAATCATAGCCAACATTGACACAGGTGTTTGGCCAGAATCAAAGAGCTTTAGTGATGAAGGGTATGGACCTGTGCCATCAAAATGGAGAGGCATTTGTCAAACTGACTCCAACTTCCTTTGCAATAGGAAGCTTATTGGAGGAAGATATTTCCACAAAGGATATGAAGCAGCCGGAGGCAAACTCAACGCCACTTTACTCACCGTACGCGACCACGACGGCCATGGAACCCACACTCTCTCAACCGCTGCCGGAAACTTTGTCACCGGAGCCAACGTTTTTGGCCATGGTAATGGCACGGCCAAAGGTGGTGCTCCGAAGGCTCGCGCCGTTGCCTACAAGGTCTGCTGGCCTCCGCTCTTTGACTCACAATGCTTCGACGCCGATATTCTTGCCGCCTTCGAAGCCGCTATTGCCGATGGAGTCGACGTGCTATCAACTTCCCTTGGTGGAGCAGCCGACGAATATTTCAACGATCCACTTGCTATCGCCGCCTTCCTCGCCGTTCAACGAGGCATTCTCGTTGTCTTCTCTGGTGGCAATTCCGGTCCTTTCCCAATGACCGTCACCAATGTTTCCCCTTGGGTATTCACCGTCGCGGCCAACACCATAGACAGAGATTTCGTCAGTTATGTGGGGCTTGGAAACAAGAAACACATCAAGGGTTTAAGCCTTTCTTCAGTTGCTTCATTACCAAAGAAGTTCTATCCATTGATCAATTCAGTGGATGCAAAATTCAGCAATGTCACCGAATTCCATGCTCAATTTTGCGGCAAGGGGACGCTTGATCCAATGAAAGTAAAGGGAAAGATAGTGATTTGTCAAGTAGGGGAGACCGAAGGAGTAGACAAGGGCTACCAAGCTTCTCTAGCTGGTGCCGCAGGAGTAATTGTAGCTAACGATATCGAAAAAGGAGACGAAATCTATCCCGAATTACACTTCATTCCCGCTTCGGATATTACGAATACCGACGCACAAATAGTCCAAAAGTATCTCAAATCCACCAAAACACCAATAGCTCATTTAACCAGTGTCAAAACATTGTTGAGTGTCAAACCAGCTCCAATCATTGCTACCTTCTCATCTAGAGGCCCCAACCCAATTGATAGTACCATTCTCAAGCCTGATGTCACAGCCCCTGGTGTGAATATACTGGCTTCTTATCCCACTGGAATTGCTCCGACGTTCTCCGCTGCTGACCGGCGGCGTATCCCATTTAATGTTATCTCTGGTACTTCCATGTCTTGTCCTCACGTTGCCGGTATCGCCGGTCTTGTTAAGTCCATTCATCCTAATTGGAGCCCTGCTGCTATCAAATCTGCTATTATGACTACTGCCAAAACAAGAGGCAACAACAACCAAACAATCCTCGACTCAACGAAACTCAAGGCCACACCTTACGCTTATGGTGCAGGACAAGTATATCCAAACGATGCAGCGGACCCCGGCCTCGTTTATGATATCACTATTAATGACTACTTAAACTTCTTGTGCGCTCGAGGCTACGATGCAATGAAAATTAAAAAATTCTATGCTAAGCCATTTAACTGTGTTAGATCGTTCAAAGTCACGGATCTCAACTACCCGTCGATCTCGGTCGGGGAGCTGAAAATTGGTGCTCCTTTGACAATCAATAGAAGAGTTAAGAACGTGGGAAGTCCAGGGACGTATGTCGCACGGGTCAAAGCGTCACCCGGTGTTGCGGTTACGATCGAGCCGAGCACATTGGTGTTTAGAAGTGTGGGTGAAGAGAAGGGGTTTAAGGTTGTATTGCAAAACACAGGAAAAGTGAAAAGTGGAGATGTGTTTGGAACATTGATTTGGTCTGATGGGAAGCACTTTGTTAGAAGTCCCATTGCTGTGCATTTGGGACCCGGCATTGGAAATTAA

Protein sequence

MSIIYTLFTLVTSFLLLFLCFLLILSILNLGKSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Homology
BLAST of IVF0001602 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 1.5e-223
Identity = 382/700 (54.57%), Postives = 509/700 (72.71%), Query Frame = 0

Query: 33  SIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIP 92
           +IFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+E++  +P
Sbjct: 74  AIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVP 133

Query: 93  SNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSNFLCNRKL 152
           S+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ F CNRKL
Sbjct: 134 SSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKL 193

Query: 153 IGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGA 212
           IG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GNGTAKGG+
Sbjct: 194 IGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGS 253

Query: 213 PKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAA 272
           P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FND +AI +
Sbjct: 254 PRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGS 313

Query: 273 FLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSL 332
           F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN KH KG SL
Sbjct: 314 FHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSL 373

Query: 333 SSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKG 392
           SS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+   V+KG
Sbjct: 374 SSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKG 433

Query: 393 YQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSV 452
              +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK PIAH+T  
Sbjct: 434 RAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPS 493

Query: 453 KTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIP 512
           +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT    D RR+ 
Sbjct: 494 RTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLL 553

Query: 513 FNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKAT 572
           FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I ++T +KAT
Sbjct: 554 FNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKAT 613

Query: 573 PYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVR-SFKVT 632
           P+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C      + 
Sbjct: 614 PFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLV 673

Query: 633 DLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEE 692
           +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L F  VGE+
Sbjct: 674 NLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQ 733

Query: 693 KGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 729
           K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 734 KTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of IVF0001602 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 763.5 bits (1970), Expect = 2.3e-219
Identity = 386/703 (54.91%), Postives = 493/703 (70.13%), Query Frame = 0

Query: 29  NLGKSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 88
           N  ++IFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT SWNF+ +  +
Sbjct: 80  NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 139

Query: 89  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNR 148
             +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C  D    CNR
Sbjct: 140 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD--VPCNR 199

Query: 149 KLIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 208
           KLIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GANVFG GNGTA 
Sbjct: 200 KLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTAS 259

Query: 209 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 268
           GG+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG A +Y +D +A
Sbjct: 260 GGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 319

Query: 269 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKG 328
           I +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V L N +  KG
Sbjct: 320 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 379

Query: 329 LSLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEG 388
            SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI++C  G+   
Sbjct: 380 TSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 439

Query: 389 VDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAH 448
           VDKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  YL STK P  +
Sbjct: 440 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 499

Query: 449 LTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADR 508
           + +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++     PT   +D 
Sbjct: 500 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 559

Query: 509 RRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTK 568
           RR PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N  + ++D + 
Sbjct: 560 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 619

Query: 569 LKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKP-FNCVRS 628
            KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F   P + C + 
Sbjct: 620 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 679

Query: 629 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 688
             + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V++EP  L F  
Sbjct: 680 ANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 739

Query: 689 VGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 729
            GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 740 TGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of IVF0001602 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 657.1 bits (1694), Expect = 2.3e-187
Identity = 361/720 (50.14%), Postives = 470/720 (65.28%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++I YSY R  NGFAA+L+++EA ++A+NP V+SVF +K  KLHTTRSW FLG+      
Sbjct: 73  EAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR--- 132

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWR-GICQTDS-----NFL 151
             NS W   +FGE+ II NIDTGVWPES+SFSD+GYG VPSKWR G+CQ +         
Sbjct: 133 GQNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNT 192

Query: 152 CNRKLIGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGT 211
           CNRKLIG RY++K +EA  G+L+  L T RD  GHGTHTLSTA GNFV GA VF  GNGT
Sbjct: 193 CNRKLIGARYYNKAFEAHNGQLDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGT 252

Query: 212 AKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA----ADEY 271
           AKGG+P+AR  AYKVCW     + C+ AD+LAA + AI DGVDV++ S G +    A+  
Sbjct: 253 AKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGI 312

Query: 272 FNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGN 331
           F D ++I AF A+ + IL+V S GN GP P TV NV+PWVFT+AA+T+DRDF S + + N
Sbjct: 313 FTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTINN 372

Query: 332 KKHIKGLSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVIC-Q 391
           +  I+G SL  V   P + + LI S DAK +N T   AQ C +GTLD  KV GKIV+C +
Sbjct: 373 QL-IEGASL-FVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTR 432

Query: 392 VGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKST 451
            G+ + V +G +A  AGA G+I+ N ++ G  +  E H     +     A+     +K+T
Sbjct: 433 EGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTT 492

Query: 452 ---------KT-PIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNIL 511
                    KT     ++  +TL   KPAP++A+FSSRGPN I  +ILKPDVTAPGVNIL
Sbjct: 493 AIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNIL 552

Query: 512 ASYPTGIAPTFSAADRRR-IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMT 571
           A+Y    + +    D RR   FNV+ GTSMSCPH +GIAGL+K+ HP+WSPAAIKSAIMT
Sbjct: 553 AAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMT 612

Query: 572 TAKTRGNNNQTILDS-TKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYD 631
           TA T  N N+ I D+  K  A  +AYG+G V P+ A +PGLVYD+++ DYLNFLCA GYD
Sbjct: 613 TATTLDNTNRPIQDAFDKTLADAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYD 672

Query: 632 AMKIKKF-YAKPFNCVRSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVK 691
              I    + + F C  S  V DLNYPSI++  L++  P+TI R V NVG P TY    +
Sbjct: 673 QQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRL-KPVTIARTVTNVGPPSTYTVSTR 732

Query: 692 ASPGVAVTIEPSTLVFRSVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 727
           +  G ++ + P +L F  +GE K FKV++Q ++   +    FG L W+DGKH VRSPI V
Sbjct: 733 SPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSPITV 786

BLAST of IVF0001602 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.6e-172
Identity = 321/703 (45.66%), Postives = 451/703 (64.15%), Query Frame = 0

Query: 34  IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 93
           + Y+Y  + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 94  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF---LCNRKL 153
             ++  A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF   LCNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 154 IGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 213
           IG R+F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 214 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 273
            AP+AR   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI
Sbjct: 245 MAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 304

Query: 274 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGL 333
            AF A++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  G+
Sbjct: 305 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 364

Query: 334 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 393
           SL    +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V 
Sbjct: 365 SLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 424

Query: 394 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 453
           KG     AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++
Sbjct: 425 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 484

Query: 454 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 513
            + T++ VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR
Sbjct: 485 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 544

Query: 514 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKL 573
           + FN+ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T  
Sbjct: 545 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 604

Query: 574 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRS 633
            +TP+ +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S
Sbjct: 605 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 664

Query: 634 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFR 693
           + V DLNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+
Sbjct: 665 YSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 694 SVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 727
              E+K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of IVF0001602 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 1.9e-165
Identity = 322/714 (45.10%), Postives = 451/714 (63.17%), Query Frame = 0

Query: 34  IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 93
           I + Y   F+GF+A++   EA+NL  +P V++VFE++ R+LHTTRS  FLG++N  G   
Sbjct: 58  IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG--- 117

Query: 94  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFL---CNRKL 153
             +W+ + +G DVII   DTG+WPE +SFSD   GP+P +WRG+C++ + F    CNRK+
Sbjct: 118 --LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 177

Query: 154 IGGRYFHKGYEAAG-GKLNATL--LTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 213
           IG R+F KG +AA  G +N T+  L+ RD DGHGTHT STAAG     A++ G+ +G AK
Sbjct: 178 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 237

Query: 214 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA---ADEYFND 273
           G APKAR  AYKVCW    DS C D+DILAAF+AA+ DGVDV+S S+GG       Y+ D
Sbjct: 238 GVAPKARIAAYKVCWK---DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLD 297

Query: 274 PLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH 333
           P+AI ++ A  +GI V  S GN GP  M+VTN++PWV TV A+TIDR+F +   LG+   
Sbjct: 298 PIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHR 357

Query: 334 IKGLSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGET 393
           ++G+SL +   L  + +P++    +  S+     A  C + TLDP +V+GKIVIC  G +
Sbjct: 358 LRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIVICDRGSS 417

Query: 394 EGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPI 453
             V KG     AG  G+I+AN    G+ +  + H IPA  + + +   ++ Y  S   PI
Sbjct: 418 PRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPI 477

Query: 454 AHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAA 513
           A +    T++ +KPAP+IA+FS RGPN +   ILKPD+ APGVNILA++   + PT   +
Sbjct: 478 ASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPS 537

Query: 514 DRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD- 573
           D R+  FN++SGTSM+CPHV+G A L+KS HP+WSPA I+SA+MTT     N+N++++D 
Sbjct: 538 DPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDE 597

Query: 574 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 633
           ST   ATPY YG+G +    A +PGLVYDIT +DY+ FLC+ GY    I+     P  C 
Sbjct: 598 STGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCP 657

Query: 634 RSFKVT--DLNYPSISV---GELKIGAPLTINRRVKNVG-SPGTYVARVKASPGVAVTIE 693
            + K +  +LNYPSI+       +     T+ R   NVG +   Y AR+++  GV VT++
Sbjct: 658 TTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVK 717

Query: 694 PSTLVFRSVGEEKGFKV-VLQNTGKV---KSGDVFGTLIWSD-GKHFVRSPIAV 727
           P  LVF S  + + + V V  NT  V   ++G VFG++ W D GKH VRSPI V
Sbjct: 718 PPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of IVF0001602 vs. ExPASy TrEMBL
Match: A0A1S4DX90 (subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=1)

HSP 1 Score: 1411.0 bits (3651), Expect = 0.0e+00
Identity = 698/703 (99.29%), Postives = 702/703 (99.86%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 151 EAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 210

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF CNRKLI
Sbjct: 211 PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLI 270

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 271 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 330

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 331 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 390

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH+KG+SLS
Sbjct: 391 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLS 450

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY
Sbjct: 451 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 510

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
           QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK
Sbjct: 511 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 570

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF
Sbjct: 571 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 630

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
           NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP
Sbjct: 631 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 690

Query: 572 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
           YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL
Sbjct: 691 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 750

Query: 632 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
           NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG
Sbjct: 751 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 810

Query: 692 FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 735
           FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 811 FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of IVF0001602 vs. ExPASy TrEMBL
Match: A0A0A0L241 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1)

HSP 1 Score: 1345.5 bits (3481), Expect = 0.0e+00
Identity = 660/704 (93.75%), Postives = 684/704 (97.16%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 48  EAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 107

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS F CNRKLI
Sbjct: 108 PSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLI 167

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 168 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 227

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 228 KARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 287

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKHIKGLSLS
Sbjct: 288 LAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLS 347

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           SV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+
Sbjct: 348 SVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGF 407

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
           QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVK
Sbjct: 408 QASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVK 467

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           TLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPF
Sbjct: 468 TLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPF 527

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
           NVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP
Sbjct: 528 NVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 587

Query: 572 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
           YAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKVTDL
Sbjct: 588 YAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDL 647

Query: 632 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
           NYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGEEKG
Sbjct: 648 NYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKG 707

Query: 692 FKVVLQNTGKVKSG-DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 735
           FKVVLQNTGKVK+G DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 708 FKVVLQNTGKVKNGSDVFGTLIWSDGKHFVRSSIAVHLGPGIGN 751

BLAST of IVF0001602 vs. ExPASy TrEMBL
Match: A0A5D3D763 (Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00150 PE=3 SV=1)

HSP 1 Score: 1341.3 bits (3470), Expect = 0.0e+00
Identity = 674/711 (94.80%), Postives = 680/711 (95.64%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 74  EAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 133

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI
Sbjct: 134 PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 193

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 194 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 253

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 254 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 313

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIK    +
Sbjct: 314 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKVHVYT 373

Query: 332 SVASL-PKKFYP-------LINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGE 391
            +  L   +F P       L N       +  E   QFCGKGTLDPMKVKGKIVICQVGE
Sbjct: 374 HLLGLGASRFAPNFICAPELYNQY--SIGSTLETSNQFCGKGTLDPMKVKGKIVICQVGE 433

Query: 392 TEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTP 451
           TEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTP
Sbjct: 434 TEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTP 493

Query: 452 IAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSA 511
           IAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSA
Sbjct: 494 IAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSA 553

Query: 512 ADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD 571
           ADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD
Sbjct: 554 ADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD 613

Query: 572 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 631
           STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV
Sbjct: 614 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 673

Query: 632 RSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVF 691
           RSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVF
Sbjct: 674 RSFKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVF 733

Query: 692 RSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 735
           RSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 734 RSVGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 782

BLAST of IVF0001602 vs. ExPASy TrEMBL
Match: A0A6J1CQS4 (subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 PE=3 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.6e-295
Identity = 497/701 (70.90%), Postives = 587/701 (83.74%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           +SIFYSYTRSFNGFAA LD+  A  LA+NP+VISVFEN+ARKLHTT SWNFLG+EN  G+
Sbjct: 72  ESIFYSYTRSFNGFAATLDENVAARLAKNPQVISVFENRARKLHTTHSWNFLGMENAQGV 131

Query: 92  -PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKL 151
            P NSIWNAAKFG+D+I+ANIDTGVWPESKSFSDEG+GP+PS+W+G CQT  NF CNRKL
Sbjct: 132 PPPNSIWNAAKFGQDIIVANIDTGVWPESKSFSDEGFGPIPSRWKGTCQTYLNFHCNRKL 191

Query: 152 IGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGA 211
           IGGRYF+KGYEAAG KL+ + LTVRDHDGHGTHTL+TAAGNFV GANVFG GNGTAKGGA
Sbjct: 192 IGGRYFYKGYEAAGAKLSPSSLTVRDHDGHGTHTLATAAGNFVPGANVFGQGNGTAKGGA 251

Query: 212 PKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAA 271
           P+AR  AYKVCWPPL + +CFDAD LA FEAAIADGVDV+STSLGG+  ++ +DPLAIAA
Sbjct: 252 PRARVAAYKVCWPPLPEGECFDADTLAGFEAAIADGVDVISTSLGGSVRDFADDPLAIAA 311

Query: 272 FLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSL 331
           F A+Q+GI+ VFS GN GP P +V+NV+PW FTVAA+TIDRDF SY+ LGNKK IKG SL
Sbjct: 312 FHAMQQGIVTVFSAGNDGPNPGSVSNVAPWKFTVAASTIDRDFASYIALGNKKRIKGASL 371

Query: 332 SSVASLPKKFYPLINSVDAKF--SNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 391
           +SVA LPKKFYPLI+SV  +    NVT++ AQFCG+GT DP KVKGKI++C  GE  G +
Sbjct: 372 ASVAPLPKKFYPLIDSVAGRIISRNVTDYRAQFCGEGTQDPAKVKGKILVCLEGEIVGFE 431

Query: 392 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 451
           KG +A   GAAG+IV ND + GD+I+PELHF+PASD+  +D Q++ +Y+ ST+ P+ HL 
Sbjct: 432 KGIEAMRVGAAGLIVVNDKDSGDDIFPELHFLPASDVKYSDRQVLTQYMDSTRNPVGHLM 491

Query: 452 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 511
            V+T L +KPAP++A FSSRGPN I+ +ILKPD+TAPGVNILA+Y +G APT S  DRRR
Sbjct: 492 KVRTELGIKPAPVMALFSSRGPNAIEKSILKPDITAPGVNILAAYSSGNAPTTSRYDRRR 551

Query: 512 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLK 571
           IPF+VISGTSMSCPH++GIA L+KSIHP WSPAAIKSAIMTTAKTR NN  +ILD TK+K
Sbjct: 552 IPFSVISGTSMSCPHISGIAALLKSIHPQWSPAAIKSAIMTTAKTRDNNLGSILDLTKVK 611

Query: 572 ATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKV 631
           ATP+AYGAG V+PNDA DPGLVYD T+ DYLNFLCARGY A +I++FYAK + C RSFK+
Sbjct: 612 ATPFAYGAGHVHPNDAMDPGLVYDTTVKDYLNFLCARGYTAKQIRRFYAKSYVCDRSFKL 671

Query: 632 TDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGE 691
           TDLNYPSISV  L +G P+T+NRRVKNVGS GTYVARVK  P V++++EPSTL F SVGE
Sbjct: 672 TDLNYPSISVSNLTMG-PVTVNRRVKNVGSLGTYVARVKVHPEVSISVEPSTLQFSSVGE 731

Query: 692 EKGFKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 729
           EK FKVV Q  GK K  G +FGTLIWSDGKHFVRSPI ++L
Sbjct: 732 EKAFKVVFQYKGKGKREGSMFGTLIWSDGKHFVRSPIVMNL 771

BLAST of IVF0001602 vs. ExPASy TrEMBL
Match: A0A6J1L3L8 (subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3 SV=1)

HSP 1 Score: 977.2 bits (2525), Expect = 3.7e-281
Identity = 471/698 (67.48%), Postives = 571/698 (81.81%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           +SI YSYTRSFNGFAAIL++KEA +LAR+P+VISV EN+ RKLHTT SW FLGVE+D GI
Sbjct: 75  ESILYSYTRSFNGFAAILNEKEAADLARSPQVISVLENRGRKLHTTNSWRFLGVEDDEGI 134

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAA+FG D+IIANIDTGVWPES SFSDEGYGP+PSKWRG C  D +F CNRKLI
Sbjct: 135 PSNSIWNAAQFGRDMIIANIDTGVWPESNSFSDEGYGPIPSKWRGTCPDDPSFRCNRKLI 194

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYF+KGY+ AGG LNAT +++RDH+GHGTHTLSTAAGNFV GAN+FGHGNGTAKGGAP
Sbjct: 195 GGRYFYKGYKFAGGVLNATSVSIRDHNGHGTHTLSTAAGNFVPGANIFGHGNGTAKGGAP 254

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KAR  AYK CWP + + +CFDAD+LAAFEAAI DGVDV+S SLGG   E+F DP+ IAAF
Sbjct: 255 KARVAAYKACWPLIPEGECFDADVLAAFEAAINDGVDVISASLGGGDQEFFRDPVGIAAF 314

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
            A Q+GI+V+FS GN GP P TV NV+PW  TVAA+T  R+FVS V LGN K +KG SLS
Sbjct: 315 HAAQQGIIVIFSAGNDGPQPETVGNVAPWEITVAASTTGRNFVSNVALGNNKILKGSSLS 374

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           SV++LP +FYPLI+SV+AKFSNV+EF A+FCGKGTL+P KVKGKI+IC  G+  GV+KGY
Sbjct: 375 SVSALP-QFYPLIDSVNAKFSNVSEFQAKFCGKGTLNPKKVKGKILICTAGKITGVEKGY 434

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
            A+ AGA G+I+A +I+  +EI PEL+F+PAS IT +D +++  Y+ ST TP+A + +V+
Sbjct: 435 NAAEAGAVGMILATNIDNLEEIKPELYFLPASGITYSDGELLDDYINSTSTPVAQIMNVR 494

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           T + + P+P++A FSSRGPNP D  ILKPD+TAPG  ILASYPT  APT S  D+RR PF
Sbjct: 495 TEVEINPSPVVAAFSSRGPNPFDKAILKPDITAPGFTILASYPTTRAPTRSGFDQRRTPF 554

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
           NV SGTSM+CPH++ IA L+KSIHP WSPAAIKSA+MTTAKT  NNN        L ATP
Sbjct: 555 NVFSGTSMACPHISAIAALLKSIHPEWSPAAIKSALMTTAKTSDNNN------LLLNATP 614

Query: 572 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
           +A+GAG V PNDA DPGLVYDIT+++YLNFLCARGY+A+++++F  +PF C +SFK  DL
Sbjct: 615 FAFGAGHVRPNDAMDPGLVYDITVDEYLNFLCARGYNAIQLRRFSKQPFVCEKSFKAIDL 674

Query: 632 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
           NYPSIS+  L + AP+TINRRVKNVG PGTYVARV+   GVA ++EPSTL F SVGEEK 
Sbjct: 675 NYPSISIPNLNVNAPVTINRRVKNVGGPGTYVARVELPEGVAASVEPSTLQFSSVGEEKA 734

Query: 692 FKVVLQNTGKVK-SGDVFGTLIWSDGKHFVRSPIAVHL 729
           F+VV+QNTG++K  G VFG L+WSDGKH V SPI+++L
Sbjct: 735 FRVVVQNTGELKHEGYVFGKLVWSDGKHSVGSPISMNL 765

BLAST of IVF0001602 vs. NCBI nr
Match: XP_016900593.1 (PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo])

HSP 1 Score: 1408 bits (3644), Expect = 0.0
Identity = 698/703 (99.29%), Postives = 702/703 (99.86%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 151 EAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 210

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF CNRKLI
Sbjct: 211 PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLI 270

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 271 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 330

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 331 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 390

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH+KG+SLS
Sbjct: 391 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHVKGVSLS 450

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY
Sbjct: 451 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 510

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
           QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK
Sbjct: 511 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 570

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF
Sbjct: 571 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 630

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
           NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP
Sbjct: 631 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 690

Query: 572 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
           YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL
Sbjct: 691 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 750

Query: 632 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
           NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG
Sbjct: 751 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 810

Query: 692 FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 734
           FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 811 FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 853

BLAST of IVF0001602 vs. NCBI nr
Match: XP_031738719.1 (uncharacterized protein LOC101212014 [Cucumis sativus])

HSP 1 Score: 1342 bits (3474), Expect = 0.0
Identity = 660/704 (93.75%), Postives = 684/704 (97.16%), Query Frame = 0

Query: 32   KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
            ++IFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 841  EAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 900

Query: 92   PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
            PSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS F CNRKLI
Sbjct: 901  PSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLI 960

Query: 152  GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
            GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 961  GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 1020

Query: 212  KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
            KARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 1021 KARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 1080

Query: 272  LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
            LAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKHIKGLSLS
Sbjct: 1081 LAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLS 1140

Query: 332  SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
            SV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+
Sbjct: 1141 SVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGF 1200

Query: 392  QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
            QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVK
Sbjct: 1201 QASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVK 1260

Query: 452  TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
            TLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPF
Sbjct: 1261 TLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPF 1320

Query: 512  NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
            NVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP
Sbjct: 1321 NVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 1380

Query: 572  YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
            YAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRSFKVTDL
Sbjct: 1381 YAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRSFKVTDL 1440

Query: 632  NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
            NYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  VGEEKG
Sbjct: 1441 NYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSRVGEEKG 1500

Query: 692  FKVVLQNTGKVKSG-DVFGTLIWSDGKHFVRSPIAVHLGPGIGN 734
            FKVVLQNTGKVK+G DVFGTLIWSDGKHFVRS IAVHLGPGIGN
Sbjct: 1501 FKVVLQNTGKVKNGSDVFGTLIWSDGKHFVRSSIAVHLGPGIGN 1544

BLAST of IVF0001602 vs. NCBI nr
Match: TYK19384.1 (subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa])

HSP 1 Score: 1338 bits (3463), Expect = 0.0
Identity = 672/709 (94.78%), Postives = 680/709 (95.91%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 74  EAIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 133

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI
Sbjct: 134 PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 193

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 194 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 253

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 254 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 313

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIK    +
Sbjct: 314 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKVHVYT 373

Query: 332 SVASL-PKKFYPLINSVDAKFSNVT-----EFHAQFCGKGTLDPMKVKGKIVICQVGETE 391
            +  L   +F P        ++  +     E   QFCGKGTLDPMKVKGKIVICQVGETE
Sbjct: 374 HLLGLGASRFAPNFICAPELYNQYSIGSTLETSNQFCGKGTLDPMKVKGKIVICQVGETE 433

Query: 392 GVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIA 451
           GVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIA
Sbjct: 434 GVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIA 493

Query: 452 HLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAAD 511
           HLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAAD
Sbjct: 494 HLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAAD 553

Query: 512 RRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDST 571
           RRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDST
Sbjct: 554 RRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDST 613

Query: 572 KLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRS 631
           KLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRS
Sbjct: 614 KLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRS 673

Query: 632 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 691
           FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS
Sbjct: 674 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 733

Query: 692 VGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 734
           VGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN
Sbjct: 734 VGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPGIGN 782

BLAST of IVF0001602 vs. NCBI nr
Match: KAE8650095.1 (hypothetical protein Csa_011146 [Cucumis sativus])

HSP 1 Score: 1309 bits (3387), Expect = 0.0
Identity = 646/695 (92.95%), Postives = 670/695 (96.40%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYTRSFNGFAA LDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI
Sbjct: 48  EAIFYSYTRSFNGFAAKLDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 107

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWN AKFGEDVI+ANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDS F CNRKLI
Sbjct: 108 PSNSIWNTAKFGEDVIVANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSTFHCNRKLI 167

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP
Sbjct: 168 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 227

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KARAVAYK CWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF
Sbjct: 228 KARAVAYKACWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 287

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
           LAVQRGILVVFSGGNSGPFPMT+ N+SPWVFTVAA+TIDR+F SYVGLGNKKHIKGLSLS
Sbjct: 288 LAVQRGILVVFSGGNSGPFPMTIANISPWVFTVAASTIDREFASYVGLGNKKHIKGLSLS 347

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           SV SLPKKF+PLINSVDAKF NVTEFHAQFCGKGTLDPMKVKGKIVICQVGET+GVDKG+
Sbjct: 348 SVPSLPKKFFPLINSVDAKFRNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETDGVDKGF 407

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
           QAS AGA GVI+AND+EKGDEI+PELHFIPASDITNTDAQIVQ YLKST+TP+AHLTSVK
Sbjct: 408 QASRAGAVGVIIANDLEKGDEIFPELHFIPASDITNTDAQIVQNYLKSTRTPMAHLTSVK 467

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           TLLSVKPAP IATFS+RGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFS  DRRRIPF
Sbjct: 468 TLLSVKPAPTIATFSARGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSPVDRRRIPF 527

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTT------AKTRGNNNQTILDST 571
           NVISGTSMSCPHVAGIAGL+KSIHPNWSPAAIKSAIMTT      AKTRGNNNQTILDST
Sbjct: 528 NVISGTSMSCPHVAGIAGLIKSIHPNWSPAAIKSAIMTTVTYSFAAKTRGNNNQTILDST 587

Query: 572 KLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRS 631
           KLKATPYAYGAGQV PNDAADPGLVYDIT+NDYLNFLCARGY+AM+IKKFYAKPF+CVRS
Sbjct: 588 KLKATPYAYGAGQVNPNDAADPGLVYDITVNDYLNFLCARGYNAMQIKKFYAKPFSCVRS 647

Query: 632 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 691
           FKVTDLNYPSISVGELKIGAPLT+NRRVKNVGSPGTYVARVKASPGVAV+IEPSTLVF  
Sbjct: 648 FKVTDLNYPSISVGELKIGAPLTMNRRVKNVGSPGTYVARVKASPGVAVSIEPSTLVFSR 707

Query: 692 VGEEKGFKVVLQNTGKVKSG-DVFGTLIWSDGKHF 719
           VGEEKGFKVVLQNTGKVK+G DVFGTLIWSDGKHF
Sbjct: 708 VGEEKGFKVVLQNTGKVKNGSDVFGTLIWSDGKHF 742

BLAST of IVF0001602 vs. NCBI nr
Match: XP_038904203.1 (uncharacterized protein LOC120090550 [Benincasa hispida])

HSP 1 Score: 1230 bits (3182), Expect = 0.0
Identity = 601/700 (85.86%), Postives = 647/700 (92.43%), Query Frame = 0

Query: 32  KSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGI 91
           ++IFYSYT SFNGFAA LD+KEA NLARNPKVISVFENKARKLHTTRSWNFLGVEND+GI
Sbjct: 74  EAIFYSYTTSFNGFAAKLDEKEAANLARNPKVISVFENKARKLHTTRSWNFLGVENDVGI 133

Query: 92  PSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNRKLI 151
           PSNSIWNAAKFG+D+IIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF CNRKLI
Sbjct: 134 PSNSIWNAAKFGQDIIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFHCNRKLI 193

Query: 152 GGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGAP 211
           GGRYF+KGY AAGG LNAT LTVRDHDGHGTHTLSTAAGNFVTGANVFGHG+GTAKGGAP
Sbjct: 194 GGRYFYKGYVAAGGTLNATSLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGSGTAKGGAP 253

Query: 212 KARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAAF 271
           KAR  AYKVCWPP   SQC DADILAAFEAA+ADGVDV+STSLGGAADEYFNDPLAIAAF
Sbjct: 254 KARVAAYKVCWPPFLGSQCLDADILAAFEAAVADGVDVISTSLGGAADEYFNDPLAIAAF 313

Query: 272 LAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSLS 331
            AVQ+G++VVFS GNSGPFPMTVTN++PW+ TVAA T+DRDF S V LGNK+   G+SLS
Sbjct: 314 HAVQQGVVVVFSAGNSGPFPMTVTNIAPWINTVAAGTVDRDFASNVALGNKE---GVSLS 373

Query: 332 SVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKGY 391
           S+A LPKKFYPLI+SV+AK SNVTEFHA+FCG+GTLDPMKVKGKIVICQVGE EGV+K Y
Sbjct: 374 SIAPLPKKFYPLIDSVNAKLSNVTEFHARFCGEGTLDPMKVKGKIVICQVGEIEGVEKSY 433

Query: 392 QASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSVK 451
           QA+ AGA GVIVANDIEKGDEIYPELHFIPASDITN DAQ++QKYL ST TP+AHLT VK
Sbjct: 434 QAARAGAVGVIVANDIEKGDEIYPELHFIPASDITNNDAQLLQKYLNSTTTPMAHLTKVK 493

Query: 452 TLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIPF 511
           TLL++KPAPIIATFSSRGPNPID  ILKPD+TAPGVNILASY TG APTFS+ D+RRIPF
Sbjct: 494 TLLNIKPAPIIATFSSRGPNPIDPFILKPDITAPGVNILASYSTGNAPTFSSNDQRRIPF 553

Query: 512 NVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKATP 571
           NVISGTSMSCPH+AGIAGL+KSIHP+WSPAAIKSAIMTTAKTRGNN QTILDSTKLKAT 
Sbjct: 554 NVISGTSMSCPHIAGIAGLLKSIHPDWSPAAIKSAIMTTAKTRGNNLQTILDSTKLKATS 613

Query: 572 YAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVRSFKVTDL 631
           YAYGAG V+PNDA DPGLVYD TI DYLNFLCARGY+AM++KKFYAKPFNCV+SFK TDL
Sbjct: 614 YAYGAGMVHPNDARDPGLVYDTTIEDYLNFLCARGYNAMEMKKFYAKPFNCVKSFKATDL 673

Query: 632 NYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEEKG 691
           NYPSISVG L+IGAP+TINRRVK+VGSPGTYVARVK SPGVAV +EP TL F SVGEEK 
Sbjct: 674 NYPSISVGVLRIGAPVTINRRVKSVGSPGTYVARVKVSPGVAVLVEPRTLQFSSVGEEKA 733

Query: 692 FKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHLGPG 731
           FKVVLQNTGKVK GDVFGTLIWSDGKHFVRSPIAVHLGPG
Sbjct: 734 FKVVLQNTGKVKRGDVFGTLIWSDGKHFVRSPIAVHLGPG 770

BLAST of IVF0001602 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 777.3 bits (2006), Expect = 1.1e-224
Identity = 382/700 (54.57%), Postives = 509/700 (72.71%), Query Frame = 0

Query: 33  SIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIP 92
           +IFYSYT+  NGFAA LD   A  ++++P+V+SVF NKA KLHTTRSW+FLG+E++  +P
Sbjct: 74  AIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTTRSWDFLGLEHNSYVP 133

Query: 93  SNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQT--DSNFLCNRKL 152
           S+SIW  A+FGED IIAN+DTGVWPESKSF DEG GP+PS+W+GICQ   D+ F CNRKL
Sbjct: 134 SSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKL 193

Query: 153 IGGRYFHKGYEAAGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKGGA 212
           IG RYF+KGY AA G LN++  + RD DGHG+HTLSTAAG+FV G ++FG GNGTAKGG+
Sbjct: 194 IGARYFNKGYAAAVGHLNSSFDSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGS 253

Query: 213 PKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAIAA 272
           P+AR  AYKVCWPP+  ++C+DAD+LAAF+AAI DG DV+S SLGG    +FND +AI +
Sbjct: 254 PRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGS 313

Query: 273 FLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGLSL 332
           F A ++ I+VV S GNSGP   TV+NV+PW  TV A+T+DR+F S + LGN KH KG SL
Sbjct: 314 FHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSL 373

Query: 333 SSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVDKG 392
           SS A    KFYP++ SV+AK  N +   AQ C  G+LDP+K KGKI++C  G+   V+KG
Sbjct: 374 SSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKG 433

Query: 393 YQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLTSV 452
              +L G  G+++ N    G+++  + H +PA+ +T+ D+  V +Y+  TK PIAH+T  
Sbjct: 434 RAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPS 493

Query: 453 KTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRRIP 512
           +T L +KPAP++A+FSS+GP+ +   ILKPD+TAPGV+++A+Y   ++PT    D RR+ 
Sbjct: 494 RTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLL 553

Query: 513 FNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTKLKAT 572
           FN ISGTSMSCPH++GIAGL+K+ +P+WSPAAI+SAIMTTA    +    I ++T +KAT
Sbjct: 554 FNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKAT 613

Query: 573 PYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCVR-SFKVT 632
           P+++GAG V PN A +PGLVYD+ I DYLNFLC+ GY+A +I  F    F C      + 
Sbjct: 614 PFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLV 673

Query: 633 DLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRSVGEE 692
           +LNYPSI+V  L   + +T++R VKNVG P  Y  +V    GV V ++P++L F  VGE+
Sbjct: 674 NLNYPSITVPNL-TSSKVTVSRTVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQ 733

Query: 693 KGFKVVL-QNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 729
           K FKV+L ++ G V  G VFG L+WSD KH VRSPI V L
Sbjct: 734 KTFKVILVKSKGNVAKGYVFGELVWSDKKHRVRSPIVVKL 772

BLAST of IVF0001602 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 763.5 bits (1970), Expect = 1.6e-220
Identity = 386/703 (54.91%), Postives = 493/703 (70.13%), Query Frame = 0

Query: 29  NLGKSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVEND 88
           N  ++IFYSY R  NGFAAILD+ EA  +A++P V+SVF NK RKLHTT SWNF+ +  +
Sbjct: 80  NAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAKN 139

Query: 89  IGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFLCNR 148
             +  +S+WN A +GED IIAN+DTGVWPESKSFSDEGYG VP++W+G C  D    CNR
Sbjct: 140 GVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEGYGAVPARWKGRCHKD--VPCNR 199

Query: 149 KLIGGRYFHKGYEA-AGGKLNATLLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 208
           KLIG RYF+KGY A  G   NA+  T RDHDGHG+HTLSTAAGNFV GANVFG GNGTA 
Sbjct: 200 KLIGARYFNKGYLAYTGLPSNASYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTAS 259

Query: 209 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLA 268
           GG+PKAR  AYKVCWPP+  ++CFDADILAA EAAI DGVDVLS S+GG A +Y +D +A
Sbjct: 260 GGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIA 319

Query: 269 IAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKG 328
           I +F AV+ G+ VV S GNSGP   TV+NV+PWV TV A+++DR+F ++V L N +  KG
Sbjct: 320 IGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKG 379

Query: 329 LSLSSVASLP-KKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEG 388
            SLS    LP +K Y LI++ DA  +N     A  C KG+LDP KVKGKI++C  G+   
Sbjct: 380 TSLSK--PLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNAR 439

Query: 389 VDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAH 448
           VDKG QA+ AGAAG+++ ND   G+EI  + H +PAS I   D + +  YL STK P  +
Sbjct: 440 VDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGY 499

Query: 449 LTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADR 508
           + +    L+ KPAP +A+FSSRGPN I   ILKPD+TAPGVNI+A++     PT   +D 
Sbjct: 500 IKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDN 559

Query: 509 RRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILDSTK 568
           RR PFN  SGTSMSCPH++G+ GL+K++HP+WSPAAI+SAIMTT++TR N  + ++D + 
Sbjct: 560 RRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESF 619

Query: 569 LKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKP-FNCVRS 628
            KA P++YG+G V PN AA PGLVYD+T  DYL+FLCA GY+   ++ F   P + C + 
Sbjct: 620 KKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQG 679

Query: 629 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKASPGVAVTIEPSTLVFRS 688
             + D NYPSI+V  L     +T+ R++KNVG P TY AR +   GV V++EP  L F  
Sbjct: 680 ANLLDFNYPSITVPNLT--GSITVTRKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNK 739

Query: 689 VGEEKGFKVVLQNTGKVKSGDVFGTLIWSDGKHFVRSPIAVHL 729
            GE K F++ L+      SG VFG L W+D  H+VRSPI V L
Sbjct: 740 TGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVVQL 776

BLAST of IVF0001602 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 1.1e-173
Identity = 321/703 (45.66%), Postives = 451/703 (64.15%), Query Frame = 0

Query: 34  IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 93
           + Y+Y  + +GF+  L  +EA++L   P VISV      +LHTTR+  FLG++      +
Sbjct: 65  LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEH----T 124

Query: 94  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNF---LCNRKL 153
             ++  A    DV++  +DTGVWPESKS+SDEG+GP+PS W+G C+  +NF   LCNRKL
Sbjct: 125 ADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKL 184

Query: 154 IGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAKG 213
           IG R+F +GYE+  G ++ +    + RD DGHGTHT STAAG+ V GA++ G+ +GTA+G
Sbjct: 185 IGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARG 244

Query: 214 GAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAADEYFNDPLAI 273
            AP+AR   YKVCW       CF +DILAA + AIAD V+VLS SLGG   +Y+ D +AI
Sbjct: 245 MAPRARVAVYKVCWL----GGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAI 304

Query: 274 AAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKHIKGL 333
            AF A++RGILV  S GN+GP   +++NV+PW+ TV A T+DRDF +   LGN K+  G+
Sbjct: 305 GAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGV 364

Query: 334 SLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGETEGVD 393
           SL    +LP K  P I + +A  SN T  +   C  GTL P KVKGKIV+C  G    V 
Sbjct: 365 SLFKGEALPDKLLPFIYAGNA--SNAT--NGNLCMTGTLIPEKVKGKIVMCDRGINARVQ 424

Query: 394 KGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPIAHLT 453
           KG     AG  G+I+AN    G+E+  + H +PA+ +      I++ Y+ +   P A ++
Sbjct: 425 KGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASIS 484

Query: 454 SVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAADRRR 513
            + T++ VKP+P++A FSSRGPN I   ILKPD+ APGVNILA++     PT  A+D RR
Sbjct: 485 ILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRR 544

Query: 514 IPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD-STKL 573
           + FN+ISGTSMSCPHV+G+A L+KS+HP WSPAAI+SA+MTTA     + + +LD +T  
Sbjct: 545 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 604

Query: 574 KATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNC--VRS 633
            +TP+ +GAG V P  A +PGL+YD+T  DYL FLCA  Y + +I+    + + C   +S
Sbjct: 605 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 664

Query: 634 FKVTDLNYPSISVGELKIGAPLTINRRVKNVGSPGTYVARVKA-SPGVAVTIEPSTLVFR 693
           + V DLNYPS +V    +GA     R V +VG  GTY  +V + + GV +++EP+ L F+
Sbjct: 665 YSVADLNYPSFAVNVDGVGA-YKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFK 724

Query: 694 SVGEEKGFKVVLQ-NTGKVKSGDVFGTLIWSDGKHFVRSPIAV 727
              E+K + V    ++ K    + FG++ WSDGKH V SP+A+
Sbjct: 725 EANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of IVF0001602 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-166
Identity = 322/714 (45.10%), Postives = 451/714 (63.17%), Query Frame = 0

Query: 34  IFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFLGVENDIGIPS 93
           I + Y   F+GF+A++   EA+NL  +P V++VFE++ R+LHTTRS  FLG++N  G   
Sbjct: 58  IVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLGLQNQKG--- 117

Query: 94  NSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSNFL---CNRKL 153
             +W+ + +G DVII   DTG+WPE +SFSD   GP+P +WRG+C++ + F    CNRK+
Sbjct: 118 --LWSESDYGSDVIIGVFDTGIWPERRSFSDLNLGPIPKRWRGVCESGARFSPRNCNRKI 177

Query: 154 IGGRYFHKGYEAAG-GKLNATL--LTVRDHDGHGTHTLSTAAGNFVTGANVFGHGNGTAK 213
           IG R+F KG +AA  G +N T+  L+ RD DGHGTHT STAAG     A++ G+ +G AK
Sbjct: 178 IGARFFAKGQQAAVIGGINKTVEFLSPRDADGHGTHTSSTAAGRHAFKASMSGYASGVAK 237

Query: 214 GGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGA---ADEYFND 273
           G APKAR  AYKVCW    DS C D+DILAAF+AA+ DGVDV+S S+GG       Y+ D
Sbjct: 238 GVAPKARIAAYKVCWK---DSGCLDSDILAAFDAAVRDGVDVISISIGGGDGITSPYYLD 297

Query: 274 PLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVGLGNKKH 333
           P+AI ++ A  +GI V  S GN GP  M+VTN++PWV TV A+TIDR+F +   LG+   
Sbjct: 298 PIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGASTIDRNFPADAILGDGHR 357

Query: 334 IKGLSLSSVASLPKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIVICQVGET 393
           ++G+SL +   L  + +P++    +  S+     A  C + TLDP +V+GKIVIC  G +
Sbjct: 358 LRGVSLYAGVPLNGRMFPVVYPGKSGMSS-----ASLCMENTLDPKQVRGKIVICDRGSS 417

Query: 394 EGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYLKSTKTPI 453
             V KG     AG  G+I+AN    G+ +  + H IPA  + + +   ++ Y  S   PI
Sbjct: 418 PRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSNEGDRIKAYASSHPNPI 477

Query: 454 AHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILASYPTGIAPTFSAA 513
           A +    T++ +KPAP+IA+FS RGPN +   ILKPD+ APGVNILA++   + PT   +
Sbjct: 478 ASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVNILAAWTDAVGPTGLPS 537

Query: 514 DRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTAKTRGNNNQTILD- 573
           D R+  FN++SGTSM+CPHV+G A L+KS HP+WSPA I+SA+MTT     N+N++++D 
Sbjct: 538 DPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMMTTTNLVDNSNRSLIDE 597

Query: 574 STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAMKIKKFYAKPFNCV 633
           ST   ATPY YG+G +    A +PGLVYDIT +DY+ FLC+ GY    I+     P  C 
Sbjct: 598 STGKSATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSIGYGPKTIQVITRTPVRCP 657

Query: 634 RSFKVT--DLNYPSISV---GELKIGAPLTINRRVKNVG-SPGTYVARVKASPGVAVTIE 693
            + K +  +LNYPSI+       +     T+ R   NVG +   Y AR+++  GV VT++
Sbjct: 658 TTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAEAVYRARIESPRGVTVTVK 717

Query: 694 PSTLVFRSVGEEKGFKV-VLQNTGKV---KSGDVFGTLIWSD-GKHFVRSPIAV 727
           P  LVF S  + + + V V  NT  V   ++G VFG++ W D GKH VRSPI V
Sbjct: 718 PPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFDGGKHVVRSPIVV 758

BLAST of IVF0001602 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 572.8 bits (1475), Expect = 4.0e-163
Identity = 324/731 (44.32%), Postives = 440/731 (60.19%), Query Frame = 0

Query: 24  ILSILNLGKSIFYSYTRSFNGFAAILDDKEAENLARNPKVISVFENKARKLHTTRSWNFL 83
           + S+ +   SI ++Y   F+GF+A L  ++A  L  +P VISV   + R LHTTRS  FL
Sbjct: 52  LASLTSSPPSIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFL 111

Query: 84  GVENDIGIPSNSIWNAAKFGEDVIIANIDTGVWPESKSFSDEGYGPVPSKWRGICQTDSN 143
           G+ +        +   + FG D++I  IDTGVWPE  SF D G GPVP KW+G C    +
Sbjct: 112 GLRS---TDKAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQD 171

Query: 144 F---LCNRKLIGGRYFHKGYEAAGGKLNAT--LLTVRDHDGHGTHTLSTAAGNFVTGANV 203
           F    CNRKL+G R+F  GYEA  GK+N T    + RD DGHGTHT S +AG +V  A+ 
Sbjct: 172 FPESACNRKLVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPAST 231

Query: 204 FGHGNGTAKGGAPKARAVAYKVCWPPLFDSQCFDADILAAFEAAIADGVDVLSTSLGGAA 263
            G+ +G A G APKAR  AYKVCW    +S C+D+DILAAF+ A+ADGVDV+S S+GG  
Sbjct: 232 LGYAHGVAAGMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVV 291

Query: 264 DEYFNDPLAIAAFLAVQRGILVVFSGGNSGPFPMTVTNVSPWVFTVAANTIDRDFVSYVG 323
             Y+ D +AI AF A+ RGI V  S GN GP  +TVTNV+PW+ TV A TIDRDF + V 
Sbjct: 292 VPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVK 351

Query: 324 LGNKKHIKGLSLSSVASL-PKKFYPLINSVDAKFSNVTEFHAQFCGKGTLDPMKVKGKIV 383
           LGN K I G+S+     L P + YPL+            + +  C +G+LDP  VKGKIV
Sbjct: 352 LGNGKMISGVSVYGGPGLDPGRMYPLV--YGGSLLGGDGYSSSLCLEGSLDPNLVKGKIV 411

Query: 384 ICQVGETEGVDKGYQASLAGAAGVIVANDIEKGDEIYPELHFIPASDITNTDAQIVQKYL 443
           +C  G      KG      G  G+I+AN +  G+ +  + H +PA+ +  +    +++Y+
Sbjct: 412 LCDRGINSRATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYI 471

Query: 444 ------KSTKTPIAHLTSVKTLLSVKPAPIIATFSSRGPNPIDSTILKPDVTAPGVNILA 503
                 +S+K P A +    T L ++PAP++A+FS+RGPNP    ILKPDV APG+NILA
Sbjct: 472 SESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILA 531

Query: 504 SYPTGIAPTFSAADRRRIPFNVISGTSMSCPHVAGIAGLVKSIHPNWSPAAIKSAIMTTA 563
           ++P  I P+   +D RR  FN++SGTSM+CPHV+G+A L+K+ HP+WSPAAI+SA++TTA
Sbjct: 532 AWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTA 591

Query: 564 KTRGNNNQTILD-STKLKATPYAYGAGQVYPNDAADPGLVYDITINDYLNFLCARGYDAM 623
            T  N+ + ++D ST   ++   YG+G V+P  A DPGLVYDIT  DY+NFLC   Y   
Sbjct: 592 YTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRT 651

Query: 624 KIKKFYAKPFNC---VRSFKVTDLNYPSISV-----GELKIGAPLTINRRVKNVG-SPGT 683
            I     +  +C    R+  V +LNYPS SV     GE K+       R V NVG S   
Sbjct: 652 NIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFI--RTVTNVGDSDSV 711

Query: 684 YVARVKASPGVAVTIEPSTLVFRSVGEEKGFKVVLQNTG-KVKSGDV---FGTLIWSDGK 729
           Y  +++   G  VT+EP  L FR VG++  F V ++ T  K+  G      G ++WSDGK
Sbjct: 712 YEIKIRPPRGTTVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGK 771

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZSP51.5e-22354.57Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... [more]
F4JXC52.3e-21954.91Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... [more]
I1N4622.3e-18750.14Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... [more]
O653511.6e-17245.66Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... [more]
O496071.9e-16545.10Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A1S4DX900.0e+0099.29subtilisin-like protease SBT5.3 OS=Cucumis melo OX=3656 GN=LOC103491001 PE=3 SV=... [more]
A0A0A0L2410.0e+0093.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G045090 PE=3 SV=1[more]
A0A5D3D7630.0e+0094.80Subtilisin-like protease SBT5.3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A6J1CQS42.6e-29570.90subtilisin-like protease SBT5.3 OS=Momordica charantia OX=3673 GN=LOC111013337 P... [more]
A0A6J1L3L83.7e-28167.48subtilisin-like protease SBT5.3 OS=Cucurbita maxima OX=3661 GN=LOC111498821 PE=3... [more]
Match NameE-valueIdentityDescription
XP_016900593.10.099.29PREDICTED: subtilisin-like protease SBT5.3 [Cucumis melo][more]
XP_031738719.10.093.75uncharacterized protein LOC101212014 [Cucumis sativus][more]
TYK19384.10.094.78subtilisin-like protease SBT5.3 [Cucumis melo var. makuwa][more]
KAE8650095.10.092.95hypothetical protein Csa_011146 [Cucumis sativus][more]
XP_038904203.10.085.86uncharacterized protein LOC120090550 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT2G04160.11.1e-22454.57Subtilisin-like serine endopeptidase family protein [more]
AT5G59810.11.6e-22054.91Subtilase family protein [more]
AT5G67360.11.1e-17345.66Subtilase family protein [more]
AT4G34980.11.3e-16645.10subtilisin-like serine protease 2 [more]
AT3G14240.14.0e-16344.32Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 176..189
score: 43.56
coord: 515..531
score: 57.35
coord: 103..122
score: 30.51
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 103..558
e-value: 9.3E-40
score: 137.1
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 105..598
e-value: 5.4E-181
score: 604.4
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 74..589
IPR003137PA domainPFAMPF02225PAcoord: 361..431
e-value: 2.6E-10
score: 40.3
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 33..75
e-value: 8.0E-10
score: 39.3
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 25..75
e-value: 3.4E-13
score: 51.8
NoneNo IPR availableGENE3D2.60.40.2310coord: 602..728
e-value: 1.6E-33
score: 117.2
NoneNo IPR availableGENE3D3.50.30.30coord: 310..454
e-value: 5.4E-181
score: 604.4
NoneNo IPR availablePANTHERPTHR10795:SF662SUBTILISIN-LIKE PROTEASE SBT5.4coord: 26..729
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 79..585
score: 29.516796
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 316..445
e-value: 8.81824E-26
score: 100.951
NoneNo IPR availableSUPERFAMILY52025PA domaincoord: 361..440
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 630..726
e-value: 1.5E-26
score: 92.5
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 26..729
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 516..526
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 73..551
e-value: 1.7582E-134
score: 397.354

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
IVF0001602.2IVF0001602.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity