IVF0001258 (gene) Melon (IVF77) v1

Overview


Name	IVF0001258
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Protease inhibitor protein
Location	tig00000135: 1215255 .. 1215755 (+)
RNA-Seq Expression	IVF0001258
Synteny	IVF0001258

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGAAGATCAATCTGCTCCAAGTAAGTTCATCCTATCAAAACGATCTTCATTTTGAGAAACTAGTTTCGATTGAGTGGTTTGTAAACTAACATGAATTTGATCAACTATCGCAAGTGTGATAGTCTTGGAAGTCAAATCAATTCACAGAAAACCAACTATCACAAGTATTTTTTGTTCTATAGGATTTAACTTTATTCATATTGAAGTTTTGTATATTTTGAGTCTGTCAGAGTATACACTTTGAATGGATTTAAGTTAATATCTATGGCAAAATTTAACATAAATATATAATTTTTAAATAGGAAAAACTCAATGGCCAGAGCTTGTCTTCATAAAATATTCTATTGCTGCCGCGATCATAGAAAGAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATACCAAACGAGTTTGGTGTGATGTCAATGTGGAAGATGTAATTGTGAAAATGCCCAAAGTTGGTTAG

mRNA sequence

ATGGCTGAAGATCAATCTGCTCCAAGAAAAACTCAATGGCCAGAGCTTGTCTTCATAAAATATTCTATTGCTGCCGCGATCATAGAAAGAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATACCAAACGAGTTTGGTGTGATGTCAATGTGGAAGATGTAATTGTGAAAATGCCCAAAGTTGGTTAG

Coding sequence (CDS)

ATGGCTGAAGATCAATCTGCTCCAAGAAAAACTCAATGGCCAGAGCTTGTCTTCATAAAATATTCTATTGCTGCCGCGATCATAGAAAGAGAGAATCCTGATGTGGAAGCTGTCAAGATTCTATCTGGTACACCTAGAATTCTGAATTTTGATACCAAACGAGTTTGGTGTGATGTCAATGTGGAAGATGTAATTGTGAAAATGCCCAAAGTTGGTTAG

Protein sequence

MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVKMPKVG

Homology

[Close]

BLAST of IVF0001258 vs. ExPASy Swiss-Prot
Match: P19873 (Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 9.2e-09
Identity = 30/67 (44.78%), Postives = 42/67 (62.69%), Query Frame = 0


Query: 6  SAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVI 65
          S P K+ WP LV +  S+A AIIER+NP+V+AV +  GTP   +F   RV   VN   ++
Sbjct: 2  SCPGKSSWPHLVGVGGSVAKAIIERQNPNVKAVILEEGTPVTKDFRCNRVRIWVNKRGLV 61

Query: 66 VKMPKVG 73
          V  P++G
Sbjct: 62 VSPPRIG 68

[Close]

BLAST of IVF0001258 vs. ExPASy Swiss-Prot
Match: P82381 (Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 3.3e-06
Identity = 25/64 (39.06%), Postives = 36/64 (56.25%), Query Frame = 0


Query: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVK 67
          P K  WPELV    ++AAA +EREN +V A+ +  G+    +F   RVW  VN   V+  
Sbjct: 5  PGKNAWPELVGKSGNMAAATVERENRNVHAIVLKEGSAMTKDFRCDRVWVIVNDHGVVTS 64

Query: 68 MPKV 72
          +P +
Sbjct: 65 VPHI 68

[Close]

BLAST of IVF0001258 vs. ExPASy Swiss-Prot
Match: Q6XNP7 (Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 9.6e-06
Identity = 28/67 (41.79%), Postives = 40/67 (59.70%), Query Frame = 0


Query: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTP--RILNFDTKRVWCDVNVEDVI 67
          P K  WPELV     IAA II+ EN +V+A+ +  G P  + LNF+  RV+ D N   V+
Sbjct: 6  PVKNSWPELVGTNGDIAAGIIQTENANVKAIVVKEGLPITQDLNFNRVRVFVDEN--RVV 65

Query: 68 VKMPKVG 73
           ++P +G
Sbjct: 66 TQVPAIG 70

[Close]

BLAST of IVF0001258 vs. ExPASy Swiss-Prot
Match: Q02214 (Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.1e-05
Identity = 25/65 (38.46%), Postives = 40/65 (61.54%), Query Frame = 0


Query: 9  RKTQWPELVFIKYSIAAAIIERENPDVEAV-KILSGTPRILNFDTKRVWCDVNVEDVIVK 68
          +K  WPEL+ +    A  II++EN  +  V  +L+G+P   +F  +RV   VNV D +V+
Sbjct: 30 KKETWPELIGVPAKFAREIIQKENSKLTNVPSVLNGSPVTKDFRCERVRLFVNVLDFVVQ 89

Query: 69 MPKVG 73
          +P+VG
Sbjct: 90 IPRVG 94

[Close]

BLAST of IVF0001258 vs. ExPASy Swiss-Prot
Match: P80211 (Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 2.8e-05
Identity = 27/67 (40.30%), Postives = 38/67 (56.72%), Query Frame = 0


Query: 5  QSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDV 64
          +  P K +WPELV      AAAIIERENP+V ++     +    +F   RVW  V+   V
Sbjct: 2  RECPGKQEWPELVGEYGYKAAAIIERENPNVRSIVKHERSGFTKDFRCDRVWVVVDSTGV 61

Query: 65 IVKMPKV 72
          +V+ P+V
Sbjct: 62 VVRTPRV 68

[Close]

BLAST of IVF0001258 vs. ExPASy TrEMBL
Match: A0A5A7U228 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001230 PE=3 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 2.8e-32
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN
Sbjct: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60

Query: 61 VEDVIVKMPKV 72
          VEDVIVKMPKV
Sbjct: 61 VEDVIVKMPKV 71

[Close]

BLAST of IVF0001258 vs. ExPASy TrEMBL
Match: A0A5A7U4L9 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001240 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.1e-28
Identity = 63/72 (87.50%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAEDQSAPRKTQWPELVF+KYS AAAIIE+ENPDV+AVKILSG  RI NFDTKRVWCDVN
Sbjct: 1  MAEDQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVN 60

Query: 61 VEDVIVKMPKVG 73
          VEDV+VK PKVG
Sbjct: 61 VEDVVVKTPKVG 72

[Close]

BLAST of IVF0001258 vs. ExPASy TrEMBL
Match: A0A0A0L8E6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 6.6e-26
Identity = 57/69 (82.61%), Postives = 65/69 (94.20%), Query Frame = 0


Query: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63
          ++SAPRKT+WPELVF+KYSIAAAIIE+ENPDVEAVKILSGTPRILNFD  RVW D+NVE+
Sbjct: 3  EESAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDINVEE 62

Query: 64 VIVKMPKVG 73
          V+VK PKVG
Sbjct: 63 VVVKKPKVG 71

[Close]

BLAST of IVF0001258 vs. ExPASy TrEMBL
Match: A0A5A7U4U0 (Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001250 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.0e-18
Identity = 45/69 (65.22%), Postives = 58/69 (84.06%), Query Frame = 0


Query: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63
          ++SA RKT+WPELVF+++S AA IIE+ENPDV+A+KIL+ +PRI NFD  RVW D NVED
Sbjct: 3  EESAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCNVED 62

Query: 64 VIVKMPKVG 73
           +VK+P VG
Sbjct: 63 KVVKVPSVG 71

[Close]

BLAST of IVF0001258 vs. ExPASy TrEMBL
Match: A0A0A0L785 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.3e-17
Identity = 42/69 (60.87%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 4  DQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVED 63
          ++SA RKT+WPELVF+ +  AA IIE+ENPDV+A+KIL  +PRI NFD  RVW D N+E+
Sbjct: 3  EESAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCNIEE 62

Query: 64 VIVKMPKVG 73
           +VK+P VG
Sbjct: 63 RVVKVPSVG 71

[Close]

BLAST of IVF0001258 vs. NCBI nr
Match: KAA0049288.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 146 bits (368), Expect = 2.89e-43
Identity = 71/71 (100.00%), Postives = 71/71 (100.00%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN
Sbjct: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60

Query: 61 VEDVIVKMPKV 71
          VEDVIVKMPKV
Sbjct: 61 VEDVIVKMPKV 71

[Close]

BLAST of IVF0001258 vs. NCBI nr
Match: KAA0049287.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 132 bits (332), Expect = 1.39e-38
Identity = 63/72 (87.50%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAEDQSAPRKTQWPELVF+KYS AAAIIE+ENPDV+AVKILSG  RI NFDTKRVWCDVN
Sbjct: 1  MAEDQSAPRKTQWPELVFVKYSTAAAIIEKENPDVKAVKILSGRFRIQNFDTKRVWCDVN 60

Query: 61 VEDVIVKMPKVG 72
          VEDV+VK PKVG
Sbjct: 61 VEDVVVKTPKVG 72

[Close]

BLAST of IVF0001258 vs. NCBI nr
Match: KGN56897.1 (hypothetical protein Csa_010510 [Cucumis sativus])

HSP 1 Score: 125 bits (314), Expect = 7.55e-36
Identity = 60/72 (83.33%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAE+ SAPRKT+WPELVF+KYSIAAAIIE+ENPDVEAVKILSGTPRILNFD  RVW D+N
Sbjct: 1  MAEE-SAPRKTEWPELVFVKYSIAAAIIEKENPDVEAVKILSGTPRILNFDINRVWVDIN 60

Query: 61 VEDVIVKMPKVG 72
          VE+V+VK PKVG
Sbjct: 61 VEEVVVKKPKVG 71

[Close]

BLAST of IVF0001258 vs. NCBI nr
Match: KAA0049286.1 (Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhibitor protein [Cucumis melo var. makuwa])

HSP 1 Score: 101 bits (252), Expect = 2.18e-26
Identity = 48/72 (66.67%), Postives = 60/72 (83.33%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAE+ SA RKT+WPELVF+++S AA IIE+ENPDV+A+KIL+ +PRI NFD  RVW D N
Sbjct: 1  MAEE-SAQRKTRWPELVFVEFSTAARIIEKENPDVKAIKILADSPRIENFDPSRVWVDCN 60

Query: 61 VEDVIVKMPKVG 72
          VED +VK+P VG
Sbjct: 61 VEDKVVKVPSVG 71

[Close]

BLAST of IVF0001258 vs. NCBI nr
Match: KAE8650337.1 (hypothetical protein Csa_010581 [Cucumis sativus])

HSP 1 Score: 92.0 bits (227), Expect = 3.16e-21
Identity = 43/69 (62.32%), Postives = 55/69 (79.71%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MAE+ SA RKT+WPELVF+ +  AA IIE+ENPDV+A+KIL  +PRI NFD  RVW D N
Sbjct: 1  MAEE-SAQRKTRWPELVFVNFCTAARIIEKENPDVKAIKILVDSPRIQNFDISRVWVDCN 60

Query: 61 VEDVIVKMP 69
          +E+ +VK+P
Sbjct: 61 IEERVVKVP 68

[Close]

BLAST of IVF0001258 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 58.5 bits (140), Expect = 2.5e-09
Identity = 30/65 (46.15%), Postives = 36/65 (55.38%), Query Frame = 0


Query: 8  PRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVK 67
          PRK  WPEL       AA +IERENP V A  IL G+P   +F   RV   V+   ++VK
Sbjct: 6  PRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIVVK 65

Query: 68 MPKVG 73
           PK G
Sbjct: 66 TPKSG 70

[Close]

BLAST of IVF0001258 vs. TAIR 10
Match: AT5G43580.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 52.0 bits (123), Expect = 2.3e-07
Identity = 30/72 (41.67%), Postives = 41/72 (56.94%), Query Frame = 0


Query: 1  MAEDQSAPRKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVN 60
          MA     P K+ WPEL+  K   A  +IERENP ++AV IL GT     F   RV+  VN
Sbjct: 28 MASICEDPGKSSWPELLGAKGEDAKEVIERENPKMKAVIILDGTVVPEIFICSRVYVWVN 87

Query: 61 VEDVIVKMPKVG 73
             ++V++P +G
Sbjct: 88 DCGIVVQIPIIG 99

[Close]

BLAST of IVF0001258 vs. TAIR 10
Match: AT3G46860.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.2 bits (108), Expect = 1.3e-05
Identity = 24/64 (37.50%), Postives = 34/64 (53.12%), Query Frame = 0


Query: 9  RKTQWPELVFIKYSIAAAIIERENPDVEAVKILSGTPRILNFDTKRVWCDVNVEDVIVKM 68
          +K +WPELV     +A   IERENP+V A+ +  G  RI NF   RV+  +     +   
Sbjct: 22 KKYRWPELVGKNGQLAKMTIERENPNVLAIVLRYGEKRIENFCCNRVFVYLGSNGQVADA 81

Query: 69 PKVG 73
          P +G
Sbjct: 82 PMIG 85

The following BLAST results are available for this feature:

BLAST of IVF0001258 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P19873	9.2e-09	44.78	Inhibitor of trypsin and hageman factor OS=Cucurbita maxima OX=3661 PE=1 SV=1	[more]
P82381	3.3e-06	39.06	Proteinase inhibitor OS=Linum usitatissimum OX=4006 PE=1 SV=1	[more]
Q6XNP7	9.6e-06	41.79	Protease inhibitor HPI OS=Hevea brasiliensis OX=3981 GN=PI1 PE=1 SV=2	[more]
Q02214	2.1e-05	38.46	Trypsin inhibitor 1 OS=Nicotiana sylvestris OX=4096 PE=2 SV=1	[more]
P80211	2.8e-05	40.30	Trypsin/subtilisin inhibitor OS=Amaranthus caudatus OX=3567 PE=1 SV=1	[more]

BLAST of IVF0001258 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U228	2.8e-32	100.00	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A5A7U4L9	4.1e-28	87.50	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L8E6	6.6e-26	82.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G142910 PE=3 SV=1	[more]
A0A5A7U4U0	1.0e-18	65.22	Protease inhibitor protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A0A0L785	4.3e-17	60.87	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G141900 PE=3 SV=1	[more]

BLAST of IVF0001258 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0049288.1	2.89e-43	100.00	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17270.1 Protease inhib...	[more]
KAA0049287.1	1.39e-38	87.50	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17271.1 Protease inhib...	[more]
KGN56897.1	7.55e-36	83.33	hypothetical protein Csa_010510 [Cucumis sativus]	[more]
KAA0049286.1	2.18e-26	66.67	Protease inhibitor protein [Cucumis melo var. makuwa] >TYK17272.1 Protease inhib...	[more]
KAE8650337.1	3.16e-21	62.32	hypothetical protein Csa_010581 [Cucumis sativus]	[more]

BLAST of IVF0001258 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	2.5e-09	46.15	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT5G43580.1	2.3e-07	41.67	Serine protease inhibitor, potato inhibitor I-type family protein	[more]
AT3G46860.1	1.3e-05	37.50	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 5..72 e-value: 1.1E-18 score: 69.4
None	No IPR available	PANTHER	PTHR33091:SF62	PROTEASE INHIBITOR PROTEIN	coord: 4..72
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 10..72 e-value: 2.2E-15 score: 57.0
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 4..72
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 7..72

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0001258.2	IVF0001258.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009611	response to wounding
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity