IVF0000993 (gene) Melon (IVF77) v1

Overview


Name	IVF0000993
Type	gene
Organism	Cucumis melo L. ssp. agrestis cv. IVF77 (Melon (IVF77) v1)
Description	Polcalcin Jun o
Location	chr10: 22953827 .. 22954870 (-)
RNA-Seq Expression	IVF0000993
Synteny	IVF0000993

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGCCATTCCACCATTTTTTAGTCTTTCTCTCTCTTCCATTTCTCTATAATTGGTAATCAACAAACTTGTAACCAATCTGAAATCCCCATTTTCCTTTACCCGTTTCAATTTTCCCCTAAATCGACAATGCTCAATTTCTCCTTCCTCTTCATCCTCAATCCTCCGATTTTCATTTCTTTCTGATATCGGTTTTGGTGTTGTAATTAATTCATCCACTGTTCTTCGCAGCAGAGAGAGAAACAATTCAACCATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCGCTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGGTTCAGAATCTCAGATCGCAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCGTCTGCTGAATTGGGCTCGATTATGACAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGAGGAATTCGATGCCGATGGTGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTATTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCGATATCTGCTGAGGAATTGCATAAGGTTCTGAGAAGCTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTTGCTGGCGGCTGAGTTTGAACACCCGCCGGTGGATTTCCAGAGGAATTTGCCAGAATTTGGATCTTTTTTTTCTTTTCCGTTCTGTGTCTGACAAAAGTACCGCAAGTTCTTCCACTATTCTTTTTTTCAATATTTTCTTCTTTGTGATTTTATCTTTTTTCTTTTATCTTTTTTCTTTATAAATGAAGTCCCTAATTTTTATTTTATTTATAAAAATGAGATCTTGTTGCATTTATTTAATAGCCAA

mRNA sequence

GTGCCATTCCACCATTTTTTAGTCTTTCTCTCTCTTCCATTTCTCTATAATTGGTAATCAACAAACTTGTAACCAATCTGAAATCCCCATTTTCCTTTACCCGTTTCAATTTTCCCCTAAATCGACAATGCTCAATTTCTCCTTCCTCTTCATCCTCAATCCTCCGATTTTCATTTCTTTCTGATATCGGTTTTGGTGTTGTAATTAATTCATCCACTGTTCTTCGCAGCAGAGAGAGAAACAATTCAACCATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCGCTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGGTTCAGAATCTCAGATCGCAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCGTCTGCTGAATTGGGCTCGATTATGACAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGAGGAATTCGATGCCGATGGTGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTATTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCGATATCTGCTGAGGAATTGCATAAGGTTCTGAGAAGCTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTTGCTGGCGGCTGAGTTTGAACACCCGCCGGTGGATTTCCAGAGGAATTTGCCAGAATTTGGATCTTTTTTTTCTTTTCCGTTCTGTGTCTGACAAAAGTACCGCAAGTTCTTCCACTATTCTTTTTTTCAATATTTTCTTCTTTGTGATTTTATCTTTTTTCTTTTATCTTTTTTCTTTATAAATGAAGTCCCTAATTTTTATTTTATTTATAAAAATGAGATCTTGTTGCATTTATTTAATAGCCAA

Coding sequence (CDS)

ATGGGATTTAGATCTCTGTTCCGAAAAAAGAAACCCTTCTCCTCCGTTAATTCTCCGCTCGTTTCCTCATCCTCCACACTCGGATCTCGATCCTCATTAGATGGTTCAGAATCTCAGATCGCAGAACTCGAGGAAGTATTCAAGAAATTCGATGTGAACGGTGACGGAAAGATCTCGTCTGCTGAATTGGGCTCGATTATGACAAGCCTAGGGCACAACGCTACAGAGGAAGAGCTCGGGAAGATGATAGAGGAATTCGATGCCGATGGTGACGGATTCATCAATTTGCACGAATTTGTGGAATTAAATACTAAAGAAATCGATCCGGAGGAATTATTGGAGAATCTGAGAGAGGCGTTTTCGGTATATGATATCGATGGAAATGGATCGATATCTGCTGAGGAATTGCATAAGGTTCTGAGAAGCTTGGGGGACGATTGTTCGGTAGCGGATTGCCGGCAGATGATCAGCGGCGTCGATAGCAACGGCGATGGAATGATTAGCTTTGAGGAGTTCAAGGTGATGATGTCGACGGGACTCTCCGTTGCTGGCGGCTGA

Protein sequence

MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVAGG

Homology

[Close]

BLAST of IVF0000993 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 8.3e-54
Identity = 103/143 (72.03%), Postives = 121/143 (84.62%), Query Frame = 0


Query: 37  ESQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IMTSLGH   EEEL K I E D  GDG+IN 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VLRSLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of IVF0000993 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.1e-40
Identity = 95/180 (52.78%), Postives = 121/180 (67.22%), Query Frame = 0


Query: 5   SLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISSAELG 64
           +LFR++    S + PL  +    G  S     E    E+E VF+KFD NGDG+IS +ELG
Sbjct: 8   ALFRRRS--GSKSPPLPQADPASGGGSPAPTPEE---EMERVFRKFDANGDGRISRSELG 67

Query: 65  SIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELN-TKEIDPEELLENLREAFSVY 124
           ++  SLGH AT++EL +M+ E DADGDGFI+L EF  LN T   D   + E+LR AF V+
Sbjct: 68  ALFESLGHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVF 127

Query: 125 DIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLSVA 184
           D DGNG+ISA EL +VL  LG+  +V  CR+MI GVD NGDG+ISFEEFKVMM+ G S A
Sbjct: 128 DADGNGTISAAELARVLHGLGEKATVQQCRRMIEGVDQNGDGLISFEEFKVMMAGGGSFA 182

[Close]

BLAST of IVF0000993 vs. ExPASy Swiss-Prot
Match: Q6L4D4 (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 GN=CML15 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.9e-35
Identity = 73/139 (52.52%), Postives = 101/139 (72.66%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVE 101
           E E VF+KFD NGDG+IS AEL ++  S+GH  T++E+ +M++E D+DGDG+I+L EF  
Sbjct: 55  ETERVFRKFDANGDGRISRAELAALFRSVGHAVTDDEVARMMQEADSDGDGYISLGEFAA 114

Query: 102 LNTKEI-DPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVD 161
           ++     D     E+LR AF V+D DGNG I+  EL +VLR +G+  +VA CR+MI GVD
Sbjct: 115 ISAPPPGDAAAAEEDLRHAFGVFDADGNGVITPAELARVLRGIGEAATVAQCRRMIDGVD 174

Query: 162 SNGDGMISFEEFKVMMSTG 180
            NGDG+I+FEEFK+MM+ G
Sbjct: 175 RNGDGLINFEEFKLMMAAG 193

[Close]

BLAST of IVF0000993 vs. ExPASy Swiss-Prot
Match: O64943 (Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-34
Identity = 79/160 (49.38%), Postives = 110/160 (68.75%), Query Frame = 0


Query: 25  STLGSRSSLDGS---ESQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGK 84
           S+ GS+S+  G    E  + ELEEVFKKFD NGDGKIS +EL  I+ SLG +  E E+  
Sbjct: 6   SSDGSKSACSGEVVMEQSVHELEEVFKKFDANGDGKISGSELADILRSLGSDVGEAEVKA 65

Query: 85  MIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLR 144
           M+EE DADGDG+++L EFV+LN K       +++L+ AF V+D D NGSISA EL   L 
Sbjct: 66  MMEEADADGDGYVSLQEFVDLNNKGAS----VKDLKNAFKVFDRDCNGSISAAELCHTLE 125

Query: 145 SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGLS 182
           S+G+ C++ + + +I  VD NGDG+IS EEF+ MM++ ++
Sbjct: 126 SVGEPCTIEESKNIIHNVDKNGDGLISVEEFQTMMTSEMT 161

[Close]

BLAST of IVF0000993 vs. ExPASy Swiss-Prot
Match: Q0DJV6 (Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 GN=CML18 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.2e-33
Identity = 75/152 (49.34%), Postives = 100/152 (65.79%), Query Frame = 0


Query: 40  IAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEF 99
           +AELE+VF+++D NGDGKIS+ EL S++ +LG      E+ +M++E D+D DGF++L EF
Sbjct: 3   MAELEQVFRRYDANGDGKISAEELASVLRALGAPLGPGEVRRMMDEMDSDRDGFVDLSEF 62

Query: 100 VELNTKEI-----------DPEELLE-NLREAFSVYDIDGNGSISAEELHKVLRSLGDDC 159
              +               D E   E  LREAF +YD D NG ISA ELH+VLR LGD C
Sbjct: 63  AAFHCGPTPAHGGKGGDAKDQEAASEAELREAFRMYDADSNGKISARELHRVLRQLGDKC 122

Query: 160 SVADCRQMISGVDSNGDGMISFEEFKVMMSTG 180
           SVADC +MI  VD++GDG ++F+EFK MM  G
Sbjct: 123 SVADCSRMIRSVDADGDGCVNFDEFKKMMGGG 154

[Close]

BLAST of IVF0000993 vs. ExPASy TrEMBL
Match: A0A5A7TBZ6 (Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold228G00230 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.3e-94
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGG 186
           SVAGG
Sbjct: 181 SVAGG 185

[Close]

BLAST of IVF0000993 vs. ExPASy TrEMBL
Match: A0A1S3CNQ0 (probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 PE=4 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.3e-94
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGG 186
           SVAGG
Sbjct: 181 SVAGG 185

[Close]

BLAST of IVF0000993 vs. ExPASy TrEMBL
Match: A0A0A0KX11 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 3.0e-91
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLD SESQI ELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEELGKMI+EFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 185
           SVAG
Sbjct: 181 SVAG 184

[Close]

BLAST of IVF0000993 vs. ExPASy TrEMBL
Match: A0A6J1JSH4 (probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC111487142 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.6e-79
Identity = 161/187 (86.10%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLGS SSL+ S +QIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+ SLGHNA EEEL KMIEEFDADGDGFINL EFVELNTKEIDPEELLE+LREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLESLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMI+GVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMINGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVAGG 186
           GLSVA G
Sbjct: 181 GLSVASG 187

[Close]

BLAST of IVF0000993 vs. ExPASy TrEMBL
Match: A0A6J1FS86 (probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC111447972 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 7.8e-79
Identity = 161/187 (86.10%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLGS SSL+ S +QIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+ SLGHNA EEEL KMIEEFDADGDGFI+L EFVELNTKEIDPEELLENLREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKV-MMST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCR+MISGVD NGDGMISFEEFKV MMST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRRMISGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVAGG 186
           GLSVA G
Sbjct: 181 GLSVADG 187

[Close]

BLAST of IVF0000993 vs. NCBI nr
Match: XP_008464718.1 (PREDICTED: probable calcium-binding protein CML25 [Cucumis melo] >KAA0040984.1 putative calcium-binding protein CML25 [Cucumis melo var. makuwa] >TYK20334.1 putative calcium-binding protein CML25 [Cucumis melo var. makuwa])

HSP 1 Score: 352 bits (904), Expect = 3.33e-122
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAGG 185
           SVAGG
Sbjct: 181 SVAGG 185

[Close]

BLAST of IVF0000993 vs. NCBI nr
Match: XP_004142374.1 (probable calcium-binding protein CML25 [Cucumis sativus] >KGN52351.1 hypothetical protein Csa_009159 [Cucumis sativus])

HSP 1 Score: 341 bits (875), Expect = 8.79e-118
Identity = 179/184 (97.28%), Postives = 181/184 (98.37%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLD SESQI ELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDDSESQITELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEELGKMI+EFDADGDGFINLHEFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMRSLGHNATEEELGKMIDEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVL+SLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLQSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 184
           SVAG
Sbjct: 181 SVAG 184

[Close]

BLAST of IVF0000993 vs. NCBI nr
Match: XP_038893457.1 (probable calcium-binding protein CML25 [Benincasa hispida])

HSP 1 Score: 332 bits (850), Expect = 5.69e-114
Identity = 175/184 (95.11%), Postives = 178/184 (96.74%), Query Frame = 0


Query: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKISS 60
           MGFRSLFRKKKPFSSVNSPLVSSSSTLG+RSS D  ++QIAELEEVFKKFDVNGDGKISS
Sbjct: 1   MGFRSLFRKKKPFSSVNSPLVSSSSTLGTRSSSDDLQAQIAELEEVFKKFDVNGDGKISS 60

Query: 61  AELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREAF 120
           AELGSIM SLGHNATEEEL KMIEEFDADGDGFINL EFVELNTKEIDPEELLENLREAF
Sbjct: 61  AELGSIMGSLGHNATEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLENLREAF 120

Query: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180
           SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL
Sbjct: 121 SVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMMSTGL 180

Query: 181 SVAG 184
           SVAG
Sbjct: 181 SVAG 184

[Close]

BLAST of IVF0000993 vs. NCBI nr
Match: KAG6600842.1 (putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 301 bits (772), Expect = 4.71e-102
Identity = 162/187 (86.63%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLGS SSL+ S +QIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+ SLGHNA EEEL KMIEEFDADGDGFI+L EFVELNTKEIDPEELLENLREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFIDLQEFVELNTKEIDPEELLENLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMM-ST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMISGVD NGDGMISFEEFKVMM ST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMISGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVAGG 185
           GLSVA G
Sbjct: 181 GLSVADG 187

[Close]

BLAST of IVF0000993 vs. NCBI nr
Match: XP_022990173.1 (probable calcium-binding protein CML25 [Cucurbita maxima])

HSP 1 Score: 301 bits (770), Expect = 9.49e-102
Identity = 161/187 (86.10%), Postives = 175/187 (93.58%), Query Frame = 0


Query: 1   MGFRSLF-RKKKPFSSVNSPLVSSSSTLGSRSSLDGSESQIAELEEVFKKFDVNGDGKIS 60
           MGF+SLF RKKKPF+SV+SPLVS+SSTLGS SSL+ S +QIAELEEVFKKFDVNGDGKIS
Sbjct: 1   MGFKSLFCRKKKPFASVDSPLVSASSTLGSGSSLEDSRAQIAELEEVFKKFDVNGDGKIS 60

Query: 61  SAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVELNTKEIDPEELLENLREA 120
           S+ELG+I+ SLGHNA EEEL KMIEEFDADGDGFINL EFVELNTKEIDPEELLE+LREA
Sbjct: 61  SSELGTILGSLGHNAKEEELAKMIEEFDADGDGFINLQEFVELNTKEIDPEELLESLREA 120

Query: 121 FSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDSNGDGMISFEEFKVMM-ST 180
           FSVYDIDGNGSI+AEELH+VL+SLGDDCSV DCRQMI+GVD NGDGMISFEEFKVMM ST
Sbjct: 121 FSVYDIDGNGSITAEELHEVLKSLGDDCSVEDCRQMINGVDRNGDGMISFEEFKVMMMST 180

Query: 181 GLSVAGG 185
           GLSVA G
Sbjct: 181 GLSVASG 187

[Close]

BLAST of IVF0000993 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 211.5 bits (537), Expect = 5.9e-55
Identity = 103/143 (72.03%), Postives = 121/143 (84.62%), Query Frame = 0


Query: 37  ESQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINL 96
           +++I ELE VFKKFDVNGDGKISS ELG+IMTSLGH   EEEL K I E D  GDG+IN 
Sbjct: 32  KTEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINF 91

Query: 97  HEFVELNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMI 156
            EFVELNTK +D  ++LENL++AFSVYDIDGNGSISAEELH+VLRSLGD+CS+A+CR+MI
Sbjct: 92  EEFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMI 151

Query: 157 SGVDSNGDGMISFEEFKVMMSTG 180
            GVD +GDG I FEEFK+MM+ G
Sbjct: 152 GGVDKDGDGTIDFEEFKIMMTMG 174

[Close]

BLAST of IVF0000993 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 140.2 bits (352), Expect = 1.7e-33
Identity = 71/142 (50.00%), Postives = 103/142 (72.54%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVE 101
           ++++VF++FD NGDGKIS  EL  ++ +L   A+ EE   M+++FD DG+GFI+L EFV 
Sbjct: 17  DIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVA 76

Query: 102 LNTKEI----DPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMIS 161
           L    I    +    + +L+EAF +YD+DGNG ISA+ELH V+++LG+ CSV DC++MIS
Sbjct: 77  LFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQDCKKMIS 136

Query: 162 GVDSNGDGMISFEEFKVMMSTG 180
            VD +GDG ++F+EFK MMS G
Sbjct: 137 KVDIDGDGCVNFDEFKKMMSNG 158

[Close]

BLAST of IVF0000993 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 138.3 bits (347), Expect = 6.4e-33
Identity = 73/143 (51.05%), Postives = 97/143 (67.83%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVE 101
           EL++VF KFD NGDGKIS +ELG++  S+G + TEEEL ++++E D D DGFIN  EF  
Sbjct: 20  ELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDIDCDGFINQEEFAT 79

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDS 161
           +        E    +REAF +YD + NG IS+ E+HKVL  LG  CSV DC +MI  VD+
Sbjct: 80  ICRSSSSAVE----IREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDCVRMIGHVDT 139

Query: 162 NGDGMISFEEFKVMMSTGLSVAG 185
           +GDG ++FEEF+ MMS+   V G
Sbjct: 140 DGDGNVNFEEFQKMMSSPELVKG 158

[Close]

BLAST of IVF0000993 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 138.3 bits (347), Expect = 6.4e-33
Identity = 72/150 (48.00%), Postives = 105/150 (70.00%), Query Frame = 0


Query: 29  SRSSLDGSESQIAELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDA 88
           SR+ L   E    ++++VF++FD N DGKIS  EL  ++ +L  NA++EE   M++EFD 
Sbjct: 6   SRNCLGSME----DIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDL 65

Query: 89  DGDGFINLHEFVEL--NTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDD 148
           DG+GFI+L EFV L   + +      + +L+EAF +YD+D NG ISA ELH V+++LG+ 
Sbjct: 66  DGNGFIDLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEK 125

Query: 149 CSVADCRQMISGVDSNGDGMISFEEFKVMM 177
           CS+ DC++MI+ VDS+GDG + FEEFK MM
Sbjct: 126 CSIQDCQRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of IVF0000993 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 134.8 bits (338), Expect = 7.0e-32
Identity = 70/137 (51.09%), Postives = 93/137 (67.88%), Query Frame = 0


Query: 42  ELEEVFKKFDVNGDGKISSAELGSIMTSLGHNATEEELGKMIEEFDADGDGFINLHEFVE 101
           EL++VF +FD NGDGKIS  ELG +  ++G + TE EL +++EE D D DG+INL EF  
Sbjct: 23  ELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGYINLDEFST 82

Query: 102 LNTKEIDPEELLENLREAFSVYDIDGNGSISAEELHKVLRSLGDDCSVADCRQMISGVDS 161
           L        E    +R+AF +YD D NG ISA ELH+VL  LG  CSV DC +MI  VD+
Sbjct: 83  LCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCTRMIGPVDA 142

Query: 162 NGDGMISFEEFKVMMST 179
           +GDG ++FEEF+ MM++
Sbjct: 143 DGDGNVNFEEFQKMMTS 155

The following BLAST results are available for this feature:

BLAST of IVF0000993 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FYK2	8.3e-54	72.03	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q8RZB5	3.1e-40	52.78	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6L4D4	3.9e-35	52.52	Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
O64943	1.5e-34	49.38	Polcalcin Jun o 2 OS=Juniperus oxycedrus OX=69008 PE=1 SV=2	[more]
Q0DJV6	6.2e-33	49.34	Probable calcium-binding protein CML18 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of IVF0000993 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TBZ6	1.3e-94	100.00	Putative calcium-binding protein CML25 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CNQ0	1.3e-94	100.00	probable calcium-binding protein CML25 OS=Cucumis melo OX=3656 GN=LOC103502536 P...	[more]
A0A0A0KX11	3.0e-91	97.28	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G627080 PE=4 SV=1	[more]
A0A6J1JSH4	4.6e-79	86.10	probable calcium-binding protein CML25 OS=Cucurbita maxima OX=3661 GN=LOC1114871...	[more]
A0A6J1FS86	7.8e-79	86.10	probable calcium-binding protein CML25 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

BLAST of IVF0000993 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008464718.1	3.33e-122	100.00	PREDICTED: probable calcium-binding protein CML25 [Cucumis melo] >KAA0040984.1 p...	[more]
XP_004142374.1	8.79e-118	97.28	probable calcium-binding protein CML25 [Cucumis sativus] >KGN52351.1 hypothetica...	[more]
XP_038893457.1	5.69e-114	95.11	probable calcium-binding protein CML25 [Benincasa hispida]	[more]
KAG6600842.1	4.71e-102	86.63	putative calcium-binding protein CML25, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022990173.1	9.49e-102	86.10	probable calcium-binding protein CML25 [Cucurbita maxima]	[more]

BLAST of IVF0000993 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	5.9e-55	72.03	EF hand calcium-binding protein family	[more]
AT5G37770.1	1.7e-33	50.00	EF hand calcium-binding protein family	[more]
AT1G73630.1	6.4e-33	51.05	EF hand calcium-binding protein family	[more]
AT1G66400.1	6.4e-33	48.00	calmodulin like 23	[more]
AT1G18210.1	7.0e-32	51.09	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Melon (IVF77) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 115..143 e-value: 6.4E-6 score: 35.7 coord: 151..179 e-value: 4.0E-5 score: 33.0 coord: 78..106 e-value: 2.5E-4 score: 30.4 coord: 42..70 e-value: 2.0E-7 score: 40.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 42..102 e-value: 7.9E-13 score: 48.7 coord: 114..176 e-value: 4.6E-13 score: 49.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 147..182 score: 12.086506
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 38..73 score: 16.717142
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 111..146 score: 15.517639
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 74..109 score: 12.839682
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 116..177 e-value: 9.85436E-20 score: 76.4325
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 42..102 e-value: 6.18307E-19 score: 74.5065
None	No IPR available	GENE3D	1.10.238.10		coord: 106..181 e-value: 3.1E-25 score: 90.3 coord: 16..105 e-value: 2.1E-25 score: 90.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..34
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..34
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 29..179
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 29..179
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 51..63
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 124..136
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 160..172
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 87..99
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 36..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
IVF0000993.2	IVF0000993.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0048767	root hair elongation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity